Molecules and Cells最新文献_第4页

Single-molecule imaging for investigating the transcriptional control 用于研究转录控制的单分子成像。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2025.100179

Insung Choi , Inwha Baek

{"title":"Single-molecule imaging for investigating the transcriptional control","authors":"Insung Choi , Inwha Baek","doi":"10.1016/j.mocell.2025.100179","DOIUrl":"10.1016/j.mocell.2025.100179","url":null,"abstract":"<div><div>Transcription is an essential biological process involving numerous factors, including transcription factors (TFs), which play a central role in this process by binding to their cognate DNA motifs. Although cells must tightly regulate the kinetics of factor association and dissociation during transcription, factor dynamics during transcription remain poorly characterized, primarily because of the reliance on ensemble experiments that average out molecular heterogeneity. Recent advances in single-molecule fluorescence imaging techniques have enabled the exploration of TF dynamics at unprecedented resolution. Findings on the temporal dynamics of individual TFs have challenged classical models and provided new insights into transcriptional regulation. Single-molecule imaging has also elucidated the assembly kinetics of transcription complexes. In this review, we describe the single-molecule fluorescence imaging methods widely used to determine factor dynamics during transcription. We highlight new findings on TF binding to chromatin, TF target search, and the assembly order of transcription complexes. Additionally, we discuss the remaining challenges in achieving a comprehensive understanding of the temporal regulation of transcription.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 2","pages":"Article 100179"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human γδ T cells in the tumor microenvironment: Key insights for advancing cancer immunotherapy 肿瘤微环境中的人γδ T细胞：推进癌症免疫治疗的关键见解。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2025.100177

Won Hyung Park , Heung Kyu Lee

{"title":"Human γδ T cells in the tumor microenvironment: Key insights for advancing cancer immunotherapy","authors":"Won Hyung Park , Heung Kyu Lee","doi":"10.1016/j.mocell.2025.100177","DOIUrl":"10.1016/j.mocell.2025.100177","url":null,"abstract":"<div><div>The role of γδ T cells in antitumor responses has gained significant attention due to their major histocompatibility complex (MHC)-independent killing mechanisms, which are functionally distinct from conventional αβ T cells. Notably, γδ tumor-infiltrating lymphocytes (TILs) have been identified as favorable prognostic markers in various cancers. However, the γδ TIL subsets, including Vδ1, Vδ2, and Vδ3, exhibit distinct prognostic implications and phenotypes within the tumor microenvironment (TME). Although the underlying mechanisms remain unclear, recent studies suggest that these subset-specific differences may arise from divergent activation pathways. Vδ1 TILs appear to be mainly activated by γδ T-cell receptor (TCR) signaling, whereas Vδ2 TILs seem to rely on alternative pathways, such as natural killer (NK) receptor-mediated activation. In addition to phenotypic studies, cancer immunotherapies, such as engineered γδ T cells, γδ T-cell engagers, and γδ TCR–based therapies, are under active development. However, despite these advancements, functional heterogeneity and limited persistence within TME remain significant challenges. Overcoming these obstacles could position γδ T-cell therapies as a transformative platform for cancer treatment. Here, we review recent findings on the prognostic significance of human γδ T cells, their phenotypic characteristics, and advances in γδ T-cell therapies, offering valuable insights for the development of novel cancer immunotherapies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 2","pages":"Article 100177"},"PeriodicalIF":3.7,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guidelines for plasma membrane protein detection by surface biotinylation 表面生物素化法检测质膜蛋白的指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-02-01 DOI: 10.1016/j.mocell.2024.100174

Jae Won Roh , Hye Won Choi , Heon Yung Gee

引用次数: 0

Optimization of FRET imaging in Arabidopsis protoplasts 拟南芥原生质体FRET成像优化

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-10 DOI: 10.1016/j.mocell.2025.100180

Gyuho Choi , Yerim Cha , Tae-Jin Kim , Gah-Hyun Lim

{"title":"Optimization of FRET imaging in Arabidopsis protoplasts","authors":"Gyuho Choi , Yerim Cha , Tae-Jin Kim , Gah-Hyun Lim","doi":"10.1016/j.mocell.2025.100180","DOIUrl":"10.1016/j.mocell.2025.100180","url":null,"abstract":"<div><div>Recent advancements in fluorescence-based biosensor technologies have enabled more precise and accurate Förster resonance energy transfer (FRET) imaging within <em>Agrobacterium</em>-mediated plant transformation systems. However, the application of FRET imaging in plant tissues remains hindered by significant challenges, particularly the time-intensive process of generating transgenic lines and the complications arising from tissue autofluorescence. In contrast, protoplast-based FRET imaging offers a rapid and efficient platform for functional screening and analysis, making it an essential tool for plant research. Nevertheless, conventional protoplast-based FRET approaches are often limited by background interference, inconsistent imaging conditions, and difficulties in quantitative analysis. Here, we present a systematic optimization of imaging conditions using the calcium biosensor D3cpv, addressing these limitations to improve both precision and efficiency in protoplast-based FRET imaging. This work serves as a practical guide for streamlining FRET imaging workflows and maximizing the utility of biosensors in plant cell studies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 3","pages":"Article 100180"},"PeriodicalIF":3.7,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142971556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide statistical evidence elucidates candidate factors of life expectancy in dogs 全基因组统计证据阐明了狗的预期寿命候选因素。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100162

Won Hee Ko , Sangil Kim , Alix Catry , Je-Yoel Cho , Seunggwan Shin

{"title":"Genome-wide statistical evidence elucidates candidate factors of life expectancy in dogs","authors":"Won Hee Ko , Sangil Kim , Alix Catry , Je-Yoel Cho , Seunggwan Shin","doi":"10.1016/j.mocell.2024.100162","DOIUrl":"10.1016/j.mocell.2024.100162","url":null,"abstract":"<div><div>It is well-established that large and heavy dogs tend to live shorter lives. In this study, we aimed to determine whether traits other than body size are associated with the life expectancy of dogs. We compiled a dataset of 20 phenotypes, including body size, lifespan, snout ratio, and shedding, into a single matrix for 149 dog breeds using data from the American Kennel Club and other peer-reviewed sources. The analysis revealed that drooling might be associated with both the lifespan and body mass index of dogs. Furthermore, a genome-wide association study with adjusted phenotypes and statistical verification methods, such as Mendelian randomization. Additionally, conducting differential gene expression analysis with the salivary gland for the 2 cases, hypersalivation/less drooling vs various body sizes, we could observe the hypersalivation-related proteins. This genetic analysis suggests that body size and drooling might be candidate factors influencing lifespan. Consequently, we identified several candidate genes, including <em>IGSF1</em>, <em>PACSIN2</em>, <em>PIK3R1</em>, and <em>MCCC2</em>, as potential genetic factors influencing longevity-related phenotypes.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100162"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover and caption 封面及标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/S1016-8478(25)00005-6

引用次数: 0

Exploring the multifaceted functions of APPL in metabolism and memory using Drosophila melanogaster 利用黑腹果蝇探索类淀粉样前体蛋白（APPL）在新陈代谢和记忆中的多方面功能。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100163

Dharmendra Kumar Nath, Youngseok Lee

{"title":"Exploring the multifaceted functions of APPL in metabolism and memory using Drosophila melanogaster","authors":"Dharmendra Kumar Nath, Youngseok Lee","doi":"10.1016/j.mocell.2024.100163","DOIUrl":"10.1016/j.mocell.2024.100163","url":null,"abstract":"<div><div>Amyloid precursor protein (APP) is a single-pass transmembrane protein abundantly expressed in the central nervous system and implicated in familial Alzheimer’s disease, a progressive neurodegenerative disorder that impairs memory. Here, we investigated the role of amyloid precursor protein-like (APPL) using the model organism <em>Drosophila melanogaster</em>. In this study, <em>Appl</em> null mutants exhibited a reduced lifespan under normal conditions and increased triglyceride levels, which were mitigated by metformin treatment. Additionally, taste-associative memory impairment in <em>Appl</em><sup><em>d</em></sup> mutants suggested APPL’s role in memory formation, which was restored by curcumin supplementation. The <em>Appl</em><sup><em>d</em></sup> mutants also displayed reduced climbing ability, which was improved by supplementation with vitamins C (ascorbic acid) and B<sub>2</sub> (riboflavin). These findings suggest that APPL is involved in metabolic regulation, cognition, climbing activity, and aging in <em>Drosophila melanogaster</em>.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100163"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity 幼鼠微生物群提取物能强烈抑制α-突触核蛋白的纤维化，保护神经母细胞瘤细胞和斑马鱼免受α-突触核蛋白的毒性。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100161

Mohaddeseh Ghorbani Shiraz , Janni Nielsen , Jeremias Widmann , Ka Hang Karen Chung , Thomas Paul Davis , Casper Rasmussen , Carsten Scavenius , Jan J. Enghild , Camille Martin-Gallausiaux , Yogesh Singh , Ibrahim Javed , Daniel E. Otzen

{"title":"Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity","authors":"Mohaddeseh Ghorbani Shiraz , Janni Nielsen , Jeremias Widmann , Ka Hang Karen Chung , Thomas Paul Davis , Casper Rasmussen , Carsten Scavenius , Jan J. Enghild , Camille Martin-Gallausiaux , Yogesh Singh , Ibrahim Javed , Daniel E. Otzen","doi":"10.1016/j.mocell.2024.100161","DOIUrl":"10.1016/j.mocell.2024.100161","url":null,"abstract":"<div><div>The clinical manifestations of Parkinson's disease (PD) are driven by aggregation of α-Synuclein (α-Syn) in the brain. However, there is increasing evidence that PD may be initiated in the gut and thence spread to the brain, eg, via the vagus nerve. Many studies link PD to changes in the gut microbiome, and bacterial amyloid has been shown to stimulate α-Syn aggregation. Yet, we are not aware of any studies reporting on a direct connection between microbiome components and α-Syn aggregation. Here, we report that soluble extract from the gut microbiome of the rats, particularly young rats transgenic for PD, shows a remarkably strong ability to inhibit <em>in vitro</em> α-Syn aggregation and keep it natively unfolded and monomeric. The active component(s) are heat-labile molecule(s) of around 30- to 100-kDa size, which are neither nucleic acid nor lipid. Proteomic analysis identified several proteins whose concentrations in different rat samples correlated with the samples’ anti-inhibitory activity, while a subsequent pull-down assay linked the protein chaperone DnaK with the inhibitory activity of young rat’s microbiome, confirmed in subsequent <em>in vitro</em> assays. Remarkably, the microbiome extracts also protected neuroblastoma SH-SY5Y cells and zebrafish embryos against α-Syn toxicity. Our study sheds new light on the gut microbiome as a potential source of protection against PD and opens up for new microbiome-based therapeutic strategies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100161"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female reproductive disease, endometriosis: From inflammation to infertility 女性生殖疾病，子宫内膜异位症：从炎症到不孕。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100164

Wonhyoung Park , Whasun Lim , Miji Kim , Hyewon Jang , Soo Jin Park , Gwonhwa Song , Sunwoo Park

引用次数: 0

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/S1016-8478(25)00007-X

引用次数: 0