Molecules and Cells最新文献_第4页

Genome-wide statistical evidence elucidates candidate factors of life expectancy in dogs 全基因组统计证据阐明了狗的预期寿命候选因素。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100162

Won Hee Ko , Sangil Kim , Alix Catry , Je-Yoel Cho , Seunggwan Shin

{"title":"Genome-wide statistical evidence elucidates candidate factors of life expectancy in dogs","authors":"Won Hee Ko , Sangil Kim , Alix Catry , Je-Yoel Cho , Seunggwan Shin","doi":"10.1016/j.mocell.2024.100162","DOIUrl":"10.1016/j.mocell.2024.100162","url":null,"abstract":"<div><div>It is well-established that large and heavy dogs tend to live shorter lives. In this study, we aimed to determine whether traits other than body size are associated with the life expectancy of dogs. We compiled a dataset of 20 phenotypes, including body size, lifespan, snout ratio, and shedding, into a single matrix for 149 dog breeds using data from the American Kennel Club and other peer-reviewed sources. The analysis revealed that drooling might be associated with both the lifespan and body mass index of dogs. Furthermore, a genome-wide association study with adjusted phenotypes and statistical verification methods, such as Mendelian randomization. Additionally, conducting differential gene expression analysis with the salivary gland for the 2 cases, hypersalivation/less drooling vs various body sizes, we could observe the hypersalivation-related proteins. This genetic analysis suggests that body size and drooling might be candidate factors influencing lifespan. Consequently, we identified several candidate genes, including <em>IGSF1</em>, <em>PACSIN2</em>, <em>PIK3R1</em>, and <em>MCCC2</em>, as potential genetic factors influencing longevity-related phenotypes.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100162"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/S1016-8478(25)00005-6

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Exploring the multifaceted functions of APPL in metabolism and memory using Drosophila melanogaster 利用黑腹果蝇探索类淀粉样前体蛋白（APPL）在新陈代谢和记忆中的多方面功能。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100163

Dharmendra Kumar Nath, Youngseok Lee

{"title":"Exploring the multifaceted functions of APPL in metabolism and memory using Drosophila melanogaster","authors":"Dharmendra Kumar Nath, Youngseok Lee","doi":"10.1016/j.mocell.2024.100163","DOIUrl":"10.1016/j.mocell.2024.100163","url":null,"abstract":"<div><div>Amyloid precursor protein (APP) is a single-pass transmembrane protein abundantly expressed in the central nervous system and implicated in familial Alzheimer’s disease, a progressive neurodegenerative disorder that impairs memory. Here, we investigated the role of amyloid precursor protein-like (APPL) using the model organism <em>Drosophila melanogaster</em>. In this study, <em>Appl</em> null mutants exhibited a reduced lifespan under normal conditions and increased triglyceride levels, which were mitigated by metformin treatment. Additionally, taste-associative memory impairment in <em>Appl</em><sup><em>d</em></sup> mutants suggested APPL’s role in memory formation, which was restored by curcumin supplementation. The <em>Appl</em><sup><em>d</em></sup> mutants also displayed reduced climbing ability, which was improved by supplementation with vitamins C (ascorbic acid) and B<sub>2</sub> (riboflavin). These findings suggest that APPL is involved in metabolic regulation, cognition, climbing activity, and aging in <em>Drosophila melanogaster</em>.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100163"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity 幼鼠微生物群提取物能强烈抑制α-突触核蛋白的纤维化，保护神经母细胞瘤细胞和斑马鱼免受α-突触核蛋白的毒性。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100161

Mohaddeseh Ghorbani Shiraz , Janni Nielsen , Jeremias Widmann , Ka Hang Karen Chung , Thomas Paul Davis , Casper Rasmussen , Carsten Scavenius , Jan J. Enghild , Camille Martin-Gallausiaux , Yogesh Singh , Ibrahim Javed , Daniel E. Otzen

{"title":"Young rat microbiota extracts strongly inhibit fibrillation of α-synuclein and protect neuroblastoma cells and zebrafish against α-synuclein toxicity","authors":"Mohaddeseh Ghorbani Shiraz , Janni Nielsen , Jeremias Widmann , Ka Hang Karen Chung , Thomas Paul Davis , Casper Rasmussen , Carsten Scavenius , Jan J. Enghild , Camille Martin-Gallausiaux , Yogesh Singh , Ibrahim Javed , Daniel E. Otzen","doi":"10.1016/j.mocell.2024.100161","DOIUrl":"10.1016/j.mocell.2024.100161","url":null,"abstract":"<div><div>The clinical manifestations of Parkinson's disease (PD) are driven by aggregation of α-Synuclein (α-Syn) in the brain. However, there is increasing evidence that PD may be initiated in the gut and thence spread to the brain, eg, via the vagus nerve. Many studies link PD to changes in the gut microbiome, and bacterial amyloid has been shown to stimulate α-Syn aggregation. Yet, we are not aware of any studies reporting on a direct connection between microbiome components and α-Syn aggregation. Here, we report that soluble extract from the gut microbiome of the rats, particularly young rats transgenic for PD, shows a remarkably strong ability to inhibit <em>in vitro</em> α-Syn aggregation and keep it natively unfolded and monomeric. The active component(s) are heat-labile molecule(s) of around 30- to 100-kDa size, which are neither nucleic acid nor lipid. Proteomic analysis identified several proteins whose concentrations in different rat samples correlated with the samples’ anti-inhibitory activity, while a subsequent pull-down assay linked the protein chaperone DnaK with the inhibitory activity of young rat’s microbiome, confirmed in subsequent <em>in vitro</em> assays. Remarkably, the microbiome extracts also protected neuroblastoma SH-SY5Y cells and zebrafish embryos against α-Syn toxicity. Our study sheds new light on the gut microbiome as a potential source of protection against PD and opens up for new microbiome-based therapeutic strategies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100161"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Female reproductive disease, endometriosis: From inflammation to infertility 女性生殖疾病，子宫内膜异位症：从炎症到不孕。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100164

Wonhyoung Park , Whasun Lim , Miji Kim , Hyewon Jang , Soo Jin Park , Gwonhwa Song , Sunwoo Park

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Editorial Board Members/Copyright

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/S1016-8478(25)00007-X

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Brief guide to immunostaining 免疫染色简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100157

Gyutae Park , Sieun S. Kim , Jiwon Shim , Seung-Jae V. Lee

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Biological functions and molecular mechanisms of MORC2 in human diseases MORC2在人类疾病中的生物学功能和分子机制。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100166

Xin Zhao , Jinfeng Miao

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Crystal structure of γ-carbonic anhydrase from the polyextremophilic bacterium Aeribacillus pallidus 多嗜极细菌苍白气杆菌γ-碳酸酐酶的晶体结构。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100165

Seung Hun Choi, Mi Sun Jin

{"title":"Crystal structure of γ-carbonic anhydrase from the polyextremophilic bacterium Aeribacillus pallidus","authors":"Seung Hun Choi, Mi Sun Jin","doi":"10.1016/j.mocell.2024.100165","DOIUrl":"10.1016/j.mocell.2024.100165","url":null,"abstract":"<div><div>The polyextremophilic bacterium <em>Aeribacillus pallidus</em> produces a thermo- and alkali-stable γ-carbonic anhydrase (γ-apCA), a homotrimeric metalloenzyme containing a zinc ion in its active site that catalyzes the reversible hydration of carbon dioxide (CO<sub>2</sub>). Here, we present the first crystal structure of γ-apCA at 1.7-Å resolution, revealing 2 trimers in the asymmetric unit. The overall structure is consistent with other γ-CAs, where each monomer adopts a prism-like structure consisting of an N-terminal left-handed β-helix and a C-terminal α-helix. The active site, located at the interface between 2 monomers, coordinates the zinc ion with 3 histidine residues (H65, H82, and H87) and a water molecule in a tetrahedral configuration. The structural comparison indicates that the amino acid composition at the active site of γ-apCA differs significantly from the prototypic γ-CA from <em>Methanosarcina thermophila</em>. This variation likely accounts for the lack of measurable CO<sub>2</sub> hydration activity in γ-apCA. Additionally, the structure reveals noncatalytic zinc and sulfate ions trapped at the trimer core and trimer-trimer noncrystallographic interfaces. These may contribute to stabilizing enzyme assembly and promoting crystal packing.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100165"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Pentraxin 3 deficiency ameliorates streptozotocin-induced pancreatic toxicity via regulating ER stress and β-cell apoptosis 戊曲霉素3缺乏通过调节内质网应激和β细胞凋亡改善链脲佐菌素诱导的胰腺毒性。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-01-01 DOI: 10.1016/j.mocell.2024.100168

Suji Kim , Ae-Rang Hwang , Sun-Hee Kim , Jae Hyang Lim , Chang-Hoon Woo

{"title":"Pentraxin 3 deficiency ameliorates streptozotocin-induced pancreatic toxicity via regulating ER stress and β-cell apoptosis","authors":"Suji Kim , Ae-Rang Hwang , Sun-Hee Kim , Jae Hyang Lim , Chang-Hoon Woo","doi":"10.1016/j.mocell.2024.100168","DOIUrl":"10.1016/j.mocell.2024.100168","url":null,"abstract":"<div><div>The long pentraxin 3 (PTX3), a marker of inflammation, has been associated with cardiovascular disease, obesity, and metabolic syndrome. Recently, elevated serum PTX3 levels have been linked to type 2 diabetes in obese patients with nonalcoholic fatty liver disease. Diabetes mellitus is a metabolic syndrome characterized by hyperglycemia resulting from insufficient insulin secretion or action. However, the precise role of PTX3 in hyperglycemia remains unclear. This study aimed to investigate the physiological roles of PTX3 in vivo. The deformation of pancreatic islets was mitigated in PTX3-deficient mice treated with streptozotocin (STZ) compared to control C57BL/6J mice. In addition, PTX3 deficiency prevented STZ-induced unfolded protein responses and pancreatic β-cell death. Immunoblotting data revealed significant inhibition of inositol-requiring protein1α and C/EBP homologous protein (CHOP) protein expression in PTX3 KO mice administered tunicamycin which is a chemical endoplasmic reticulum stress inducer. Similarly, tunicamycin-induced Grp78, Grp94, ATF6, and CHOP mRNA levels were reduced in PTX3 KO mice. Moreover, recombinant PTX3–induced CHOP expression and β-cell apoptosis in primary mouse islets. These findings suggest that PTX3 plays a critical role in STZ-induced deformation of pancreatic islets via regulating endoplasmic reticulum stress and β-cell apoptosis.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 1","pages":"Article 100168"},"PeriodicalIF":3.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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