Molecules and Cells最新文献_第5页

Decoding genomic rearrangements for cancer driver discovery 解码癌症中的基因组重排：新驱动发现之路。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-08-30 DOI: 10.1016/j.mocell.2025.100272

Enyoung Seo , Sooyeon Park , Inho Park , Jinhyuk Bhin

引用次数: 0

Charge-dependent localization of Toll-like receptor 5 at the plasma membrane toll样受体5在质膜上的电荷依赖定位。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-09-05 DOI: 10.1016/j.mocell.2025.100275

Ji-Won Huh , Kwangcheon Park , Hyun-Sup Song , Seongryong Kim , You-Me Kim

{"title":"Charge-dependent localization of Toll-like receptor 5 at the plasma membrane","authors":"Ji-Won Huh , Kwangcheon Park , Hyun-Sup Song , Seongryong Kim , You-Me Kim","doi":"10.1016/j.mocell.2025.100275","DOIUrl":"10.1016/j.mocell.2025.100275","url":null,"abstract":"<div><div>Proper subcellular localization of Toll-like receptors (TLRs) is essential for initiating appropriate innate immune responses against pathogens while avoiding self-reactivity. Unc-93 homolog B1 (UNC93B1) is known to mediate the intracellular trafficking of nucleotide-sensing TLRs such as TLR9 which undergoes rapid internalization into endolysosomes upon reaching the cell surface. We previously demonstrated that UNC93B1 also facilitates the plasma membrane localization of TLR5, a sensor for bacterial flagellin. Unlike TLR9, TLR5 remained stably at the cell surface under steady-state conditions, suggesting the involvement of distinct sorting mechanisms. Using mutagenesis-based approaches, we found that the cytoplasmic domain of TLR5 is required for its surface retention, whereas the cytoplasmic domain of TLR9 is dispensable for internalization. Notably, TLR5 contains polybasic residues in its C-terminal region, absent in other TLRs. Deletion or alanine substitution of these residues led to constitutive endocytosis of TLR5. Conversely, appending the TLR5 C-terminal region to the C-terminus of TLR9 promoted its surface accumulation. Moreover, when the TLR5 C-terminal sequence was fused to a cytosolic protein along with a myristoylation motif, it mediated membrane association of the cytosolic protein in a charge-dependent manner. We further found that this region can directly interact with phosphatidic acid, an anionic phospholipid enriched in the plasma membrane. These findings reveal an electrostatic mechanism by which TLR5 is selectively retained at the plasma membrane, providing new insight into receptor-specific localization of TLRs.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 11","pages":"Article 100275"},"PeriodicalIF":6.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune cell isolation from lymphoid and nonlymphoid organs 淋巴和非淋巴器官的免疫细胞分离。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-09-19 DOI: 10.1016/j.mocell.2025.100277

Jong Seok Park, Yoontae Lee

引用次数: 0

Cover and caption 封面及标题

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-10-29 DOI: 10.1016/S1016-8478(25)00116-5

引用次数: 0

Brief guide to detecting ferroptosis 检测铁下垂的简要指南。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-09-10 DOI: 10.1016/j.mocell.2025.100276

Thu-Hang Thi Nghiem , Fedho Kusuma , Jeongmin Park , Yeonsoo Joe , Hun Taeg Chung , Jaeseok Han

引用次数: 0

Dual SMAD inhibition as a versatile platform in human pluripotent stem cell–based regenerative medicine and disease modeling 双smad抑制作为人类多能干细胞神经科学和再生医学的通用平台。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-10-03 DOI: 10.1016/j.mocell.2025.100284

Lesly Puspita , Magdalena Deline , Jae-won Shim

{"title":"Dual SMAD inhibition as a versatile platform in human pluripotent stem cell–based regenerative medicine and disease modeling","authors":"Lesly Puspita , Magdalena Deline , Jae-won Shim","doi":"10.1016/j.mocell.2025.100284","DOIUrl":"10.1016/j.mocell.2025.100284","url":null,"abstract":"<div><div>Dual SMAD inhibition is a robust and widely adopted protocol for directing human pluripotent stem cells (hPSCs) toward neuronal lineages by blocking transforming growth factor–beta and bone morphogenetic protein pathways. Suppressing transforming growth factor–beta and bone morphogenetic protein signaling enables efficient and reproducible induction of neuroectoderm, serving as the foundation for generating diverse brain region–specific neuronal subtypes. This review outlines the mechanistic basis and major achievements of the dual SMAD inhibition strategy, including its application in 2 recent clinical trials for Parkinson’s disease, and its role in preclinical studies targeting conditions, such as spinal cord injury (SCI), retinal degeneration, and amyotrophic lateral sclerosis (ALS). In addition to its significant contribution to the generation of transplantation-ready grafts from hPSCs, the protocol serves as a valuable platform for disease modeling across various neurological and metabolic disorders. The key strengths include high efficiency, technical simplicity that enables precise control of cell fate using small molecules, versatility in both 2- and 3-dimensional culture systems, and reproducibility across various hPSC lines. This review also addresses key limitations, such as restricted gliogenic capacity and limited neural progenitor cell expansion. Future research should focus on incorporating emerging technologies to advance stem cell–based applications. Overall, dual SMAD inhibition represents a powerful and versatile platform for stem cell–based neuroscience and regenerative medicine.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 11","pages":"Article 100284"},"PeriodicalIF":6.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145233081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-10-29 DOI: 10.1016/S1016-8478(25)00117-7

引用次数: 0

Post ingestive systemic nutrient sensing for whole-body homeostasis 对全身平衡的营养感知：超越细胞水平。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-08-29 DOI: 10.1016/j.mocell.2025.100271

Gahbien Lee , Jiyeon Lee , Greg S.B. Suh , Yangkyun Oh

引用次数: 0

Current therapeutic strategies in Parkinson’s disease: Future perspectives 帕金森病的当前治疗策略：未来展望。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-11-01 Epub Date: 2025-09-04 DOI: 10.1016/j.mocell.2025.100274

Tae Young Kim , Byoung Dae Lee

{"title":"Current therapeutic strategies in Parkinson’s disease: Future perspectives","authors":"Tae Young Kim , Byoung Dae Lee","doi":"10.1016/j.mocell.2025.100274","DOIUrl":"10.1016/j.mocell.2025.100274","url":null,"abstract":"<div><div>Parkinson’s disease (PD) is a progressive neurodegenerative disorder characterized by the loss of dopaminergic neurons and the accumulation of misfolded α-synuclein. Current treatments, including dopaminergic medications and deep brain stimulation, provide symptomatic relief but do not halt disease progression. Recent advances in molecular research have enabled the development of disease-modifying strategies targeting key pathogenic mechanisms, such as α-synuclein aggregation, mitochondrial dysfunction, and genetic mutations, including <em>LRRK2</em> and <em>GBA1</em>. In parallel, pluripotent stem cell-derived dopaminergic neurons have emerged as a scalable and ethically viable source for cell replacement therapy. Early-phase clinical trials have demonstrated the safety and functional integration of these grafts. Ongoing research is now focused on enhancing graft purity, immune compatibility, and anatomical precision, including homotopic transplantation and circuit-level reconstruction. Together, these emerging strategies offer the potential to shift PD treatment paradigms by combining symptomatic control with long-term neural restoration. This review summarizes current therapeutic approaches and highlights recent advances in disease-modifying and regenerative interventions for PD.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 11","pages":"Article 100274"},"PeriodicalIF":6.5,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145008334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and application of bioparts for plant synthetic biology 植物合成生物学启动子和终止子的鉴定与应用。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-10-01 Epub Date: 2025-09-01 DOI: 10.1016/j.mocell.2025.100273

Hyunjin Koo , Minah Jung , Sangwoo Lee , Sangjin Go , Yong-Min Kim

{"title":"Identification and application of bioparts for plant synthetic biology","authors":"Hyunjin Koo , Minah Jung , Sangwoo Lee , Sangjin Go , Yong-Min Kim","doi":"10.1016/j.mocell.2025.100273","DOIUrl":"10.1016/j.mocell.2025.100273","url":null,"abstract":"<div><div>Plant synthetic biology is an emerging field that combines bioinformatics, computational gene circuit design, and plant science. It has the potential to be applied in various areas, including the production of pharmaceuticals, vaccines, biofuels, and various biomaterials, including plant natural products. This review highlights recent advancements in plant synthetic biology, particularly in the development and application of biological parts such as promoters and terminators, which play a crucial role in precise gene expression regulation. Furthermore, this review clarified the identification and utilization of bidirectional promoters, which are essential for gene pyramiding, and the significance of maintaining a balance between promoter and terminator combinations for the stability of transgene expression. Furthermore, large-scale identification of promoters using Assay for Transposase-Accessible Chromatin using sequencing and Self-Transcribing Active Regulatory Region sequencing, as well as deep-learning-based models for predicting promoter regions and their transcriptional activity, are discussed. This review provides insights into the identification and application of bioparts in plant synthetic biology to achieve efficient and precise gene regulation.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 10","pages":"Article 100273"},"PeriodicalIF":6.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144993005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0