Brief guide to gene cloning

IF 6.5 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2025-05-29 DOI:10.1016/j.mocell.2025.100234

Woojin Hong, Seokjun G. Ha, Hyunwoo C. Kwon, Seung-Jae V. Lee

引用次数: 0

Abstract

Analysis and manipulation of DNA is fundamental to understand gene function and expression. Gene cloning is a routine and versatile technique for molecular biology, allowing isolation, amplification, and production of recombinant DNA molecules. Here, we provide an overall process, various types, and applications of gene cloning. This concise guide will be useful for researchers who are unfamiliar with gene cloning, focusing on key principles and experimental considerations for efficient DNA analysis.

查看原文本刊更多论文

基因克隆的简要指南。

分析和操作DNA是理解基因功能和表达的基础。基因克隆是分子生物学的常规和通用技术，允许分离，扩增和生产重组DNA分子。本文介绍了基因克隆的总体过程、类型和应用。这个简明的指南将是有用的研究人员谁是不熟悉基因克隆，重点是关键原则和实验考虑高效的DNA分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is "Mol. Cells". Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.