Molecules and Cells最新文献_第8页

Cover and caption 封面及标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-12-01 DOI: 10.1016/S1016-8478(24)00194-8

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E2F3a activates Gadd45b gene expression through PPARα-mediated transcriptional regulation in hepatic cells E2F3a 通过 PPARα 介导的肝细胞转录调控激活 Gadd45b 基因表达

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-12-01 DOI: 10.1016/j.mocell.2024.100148

Min Seok Woo , Atif Ali Khan Khalil , Frank J. Gonzalez , Jung-Hwan Kim

{"title":"E2F3a activates Gadd45b gene expression through PPARα-mediated transcriptional regulation in hepatic cells","authors":"Min Seok Woo , Atif Ali Khan Khalil , Frank J. Gonzalez , Jung-Hwan Kim","doi":"10.1016/j.mocell.2024.100148","DOIUrl":"10.1016/j.mocell.2024.100148","url":null,"abstract":"<div><div>Growth arrest and DNA damage-inducible beta (GADD45b) plays a critical role in intracellular events such as cell growth and apoptosis. Although the functional study of GADD45b has been conducted, the mechanism for the transcriptional regulation of GADD45b is largely unknown. Due to the drastic induction of hepatic GADD45b mRNA by peroxisome proliferator-activated receptor alpha activation in wild-type mice, we investigated a key factor that affects the upregulation of GADD45b mRNA. Interestingly, we found that GADD45b-promoter luciferase activity was dramatically increased as the 5′-flanking regions were closer to the transcription start site in Hepa1c1c7 cells. Additionally, we found 2 E2F (E2F-myc activator/cell cycle regulator) binding motifs in the region (−150/−1) of the GADD45b promoter. Notably, E2F3 mRNA was significantly increased only in wild-type mice treated with WY-14643, a peroxisome proliferator-activated receptor alpha agonist, followed by the induction of GADD45b mRNA. Furthermore, gene functional studies and Chromatin Immunoprecipitation assays revealed that E2F3 could regulate the GADD45b gene via the E2F binding motif. Thus, we suggest that E2F3 may play a key role in regulating the GADD45b gene as a positive transcription factor.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100148"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Editorial Board Members/Copyright 编辑委员会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-12-01 DOI: 10.1016/S1016-8478(24)00196-1

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Roles of extracellular vesicles from mesenchymal stem cells in regeneration 间充质干细胞细胞外囊泡在再生中的作用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-12-01 DOI: 10.1016/j.mocell.2024.100151

Hyeseong Jung , Yuyeon Jung , Junsik Seo , Yeongju Bae , Han-Soo Kim , Wooyoung Jeong

{"title":"Roles of extracellular vesicles from mesenchymal stem cells in regeneration","authors":"Hyeseong Jung , Yuyeon Jung , Junsik Seo , Yeongju Bae , Han-Soo Kim , Wooyoung Jeong","doi":"10.1016/j.mocell.2024.100151","DOIUrl":"10.1016/j.mocell.2024.100151","url":null,"abstract":"<div><div>Mesenchymal stem cells (MSCs) are highly valued in regenerative medicine due to their ability to self-renew and differentiate into various cell types. Their therapeutic benefits are primarily due to their paracrine effects, in particular through extracellular vesicles (EVs), which are related to intercellular communication. Recent advances in EV production and extraction technologies highlight the potential of MSC-derived EVs (MSC-EVs) in tissue engineering and regenerative medicine. MSC-EVs offer several advantages over traditional cell therapies, including reduced toxicity and immunogenicity compared with whole MSCs. EVs carrying functional molecules such as growth factors, cytokines, and miRNAs play beneficial roles in tissue repair, fibrosis treatment, and scar prevention by promoting angiogenesis, skin cell migration, proliferation, extracellular matrix remodeling, and reducing inflammation. Despite the potential of MSC-EVs, there are several limitations to their use, including variability in quality, the need for standardized methods, low yield, and concerns about the composition of EVs and the potential risks. Overall, MSC-EVs are a promising alternative to cell-based therapies, and ongoing studies aim to understand their actions and optimize their use for better clinical outcomes in wound healing and skin regeneration.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100151"},"PeriodicalIF":3.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Multiplexed multimodal single-cell technologies: From observation to perturbation analysis 复用多模式单细胞技术：从观察到扰动分析

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-08 DOI: 10.1016/j.mocell.2024.100147

Su-Hyeon Lee , Junha Park , Byungjin Hwang

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Periodontitis and atherosclerotic cardiovascular disease 牙周炎与动脉粥样硬化性心血管疾病。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-06 DOI: 10.1016/j.mocell.2024.100146

June Yeon Kim, Kyeongho Lee, Moon Geon Lee, Sung-Jin Kim

{"title":"Periodontitis and atherosclerotic cardiovascular disease","authors":"June Yeon Kim, Kyeongho Lee, Moon Geon Lee, Sung-Jin Kim","doi":"10.1016/j.mocell.2024.100146","DOIUrl":"10.1016/j.mocell.2024.100146","url":null,"abstract":"<div><div>Atherosclerotic cardiovascular disease (ASCVD) is a major global health concern linked to significant morbidity and mortality. Recent research has explored the relationship between ASCVD and periodontitis, a prevalent inflammatory oral condition. Epidemiological studies have suggested a strong association between periodontitis and ASCVD, even proposing that periodontal disease could be a modifiable risk factor for cardiovascular conditions. This review critically analyzes the current evidence for a potential causal role for periodontitis in ASCVD. While randomized controlled trials have demonstrated reductions in surrogate markers of cardiovascular risk following periodontal interventions, these studies remain inconclusive regarding their direct effects on cardiovascular events. Preclinical studies in animal models have suggested a potential causal relationship between periodontitis and ASCVD, proposing several biological mechanisms to explain this connection. These studies, however, are limited in their ability to definitively prove causality. The positive associations observed in epidemiological studies between periodontitis and ASCVD may also be influenced by various biases, such as confounding and collider stratification. Moreover, our systematic review of Mendelian randomization studies on the causal relationship between periodontitis and ASCVD found no evidence of a genetic causality, further challenging the causal hypothesis. This review underscores the need for further high-quality research clarifying the relationship between periodontitis and ASCVD to better guide clinical practice and public health policy.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100146"},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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TMEM175 plays a crucial role in osteoblast differentiation by regulating lysosomal function and autophagy TMEM175 通过调控溶酶体功能和自噬在成骨细胞分化过程中发挥关键作用

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100127

Seung Hye Lee, Ji Sun Jang, Shenzheng Mo, Hong-Hee Kim

{"title":"TMEM175 plays a crucial role in osteoblast differentiation by regulating lysosomal function and autophagy","authors":"Seung Hye Lee, Ji Sun Jang, Shenzheng Mo, Hong-Hee Kim","doi":"10.1016/j.mocell.2024.100127","DOIUrl":"10.1016/j.mocell.2024.100127","url":null,"abstract":"<div><div>Bone provides structural support, enables movement, protects internal organs, regulates calcium and phosphorus levels, and contains bone marrow essential for hematopoiesis. Osteoblasts are specialized cells responsible for bone formation through the secretion of extracellular matrix components. Transmembrane protein 175 (TMEM175), which functions as an endosomal/lysosomal K<sup>+</sup> channel and a lysosomal H<sup>+</sup> channel, regulates lysosomal function and autophagy. Despite the recognized importance of lysosomes and autophagy in osteoblast differentiation, the specific role of TMEM175 in osteoblast differentiation has not been revealed. In this study, we investigated whether TMEM175 is associated with human bone mineral density and fracture and examined the role of TMEM175 in osteoblast differentiation. In analyses of single nucleotide polymorphisms of pore ion channel genes using the mouse2human database, a significant correlation between TMEM175 single nucleotide polymorphisms and human bone mineral density and fracture was identified. TMEM175 expression levels were found to increase during osteoblast differentiation from bone chip-derived mesenchymal stem cells (BMSCs). Knockdown of TMEM175 in BMSCs suppressed osteoblast differentiation, as evidenced by decreased matrix mineralization and lower expression levels of osteoblast marker genes. Further analysis indicated that TMEM175 deficiency leads to lysosomal dysfunction and partially impairs autophagic clearance during osteoblast differentiation. Moreover, the TMEM175 inhibitor 4-aminopyridine decreased osteoblast differentiation of BMSCs. Taken together, this study reveals that TMEM175 plays an important role in osteoblast differentiation by regulating lysosomal function and autophagic clearance.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 11","pages":"Article 100127"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cover and caption 封面和标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/S1016-8478(24)00178-X

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Editorial Board Members/Copyright 编委会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/S1016-8478(24)00179-1

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Cellular and metabolic function of GIRK1 potassium channels expressed by arcuate POMC and NPY/AgRP neurons 弓状 POMC 和 NPY/AgRP 神经元表达的 GIRK1 钾通道的细胞和代谢功能。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100122

Yeeun Choi, Eun-Seon Yoo, Youjin Oh, Jong-Woo Sohn

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