Molecules and Cells最新文献_第8页

RNA-associated nuclear condensates: Where the nucleus keeps its RNAs in check rna相关核凝聚物：细胞核控制rna的地方。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-15 DOI: 10.1016/j.mocell.2025.100240

Doyeong Ku , Yewon Yang , Yoosik Kim

{"title":"RNA-associated nuclear condensates: Where the nucleus keeps its RNAs in check","authors":"Doyeong Ku , Yewon Yang , Yoosik Kim","doi":"10.1016/j.mocell.2025.100240","DOIUrl":"10.1016/j.mocell.2025.100240","url":null,"abstract":"<div><div>Noncoding RNAs (ncRNAs) are emerging regulators of cellular processes and stress response by controlling chromatin architecture and gene expression. Recent studies have revealed that these ncRNAs functions are closely associated with nuclear condensates, membrane-less compartments formed by liquid-liquid phase separation (LLPS) that provide a specialized subnuclear environment for genome organization as well as transcriptional and post-transcriptional regulation. Disruption of RNA-mediated nuclear condensates is increasingly linked to human diseases, including neurodegenerative disease and cancer, emphasizing their essential role in maintaining cellular homeostasis. Advances in high-resolution microscopy and high-throughput sequencing have elucidated subcompartmental structures and genetic components of nuclear condensates by identifying associated RNA molecules and providing the details of RNA-protein interactions at a single-nucleotide resolution. We now understand that ncRNAs act as scaffolds that recruit RNA-binding proteins with intrinsically disordered domains to trigger the nucleation event for LLPS, resulting in the formation of nuclear condensates. In this review, we summarize how RNAs and RNA-binding proteins contribute to RNA-mediated nuclear condensate formation via LLPS and support their regulatory functions.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100240"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A brief guide to analyzing TurboID-based proximity labeling-mass spectrometry in plants 基于turboid的植物接近标记-质谱分析简介。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-03 DOI: 10.1016/j.mocell.2025.100236

Tae-Ki Park , Tae-Wuk Kim

引用次数: 0

Optogenetics to biomolecular phase separation in neurodegenerative diseases 神经退行性疾病的光遗传学与生物分子相分离。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-22 DOI: 10.1016/j.mocell.2025.100247

Kyung Hwan Park, Kyung Won Kim

{"title":"Optogenetics to biomolecular phase separation in neurodegenerative diseases","authors":"Kyung Hwan Park, Kyung Won Kim","doi":"10.1016/j.mocell.2025.100247","DOIUrl":"10.1016/j.mocell.2025.100247","url":null,"abstract":"<div><div>Neurodegenerative diseases involve toxic protein aggregation. Recent evidence suggests that biomolecular phase separation, a process in which proteins and nucleic acids form dynamic, liquid-like condensates, plays a key role in this aggregation. Optogenetics, originally developed to control neuronal activity with light, has emerged as a powerful tool to investigate phase separation in living systems. This is achieved by fusing disease-associated proteins to light-sensitive oligomerization domains, enabling researchers to induce or reverse condensate formation with precise spatial and temporal control. This review highlights how optogenetic systems such as OptoDroplet are being used to dissect the mechanisms of neurodegenerative disease. We examine how these tools have been applied in models of neurodegenerative diseases, such as amyotrophic lateral sclerosis, Alzheimer’s, Parkinson’s, and Huntington’s disease. These studies implicate small oligomeric aggregates as key drivers of toxicity and highlight new opportunities for therapeutic screening. Finally, we discuss advances in light-controlled dissolution of condensates and future directions for applying optogenetics to combat neurodegeneration. By enabling precise, dynamic control of protein phase behavior in living systems, optogenetic approaches provide a powerful framework for elucidating disease mechanisms and informing the development of targeted therapies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100247"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of mitochondrial membrane potential, ROS, and calcium 线粒体膜电位、活性氧和钙的分析。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-16 DOI: 10.1016/j.mocell.2025.100238

Hye-Kyung Park, Byoung Heon Kang

引用次数: 0

Brief guide to assays for measuring health parameters using Caenorhabditis elegans 用秀丽隐杆线虫测定健康参数的试验指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-05-26 DOI: 10.1016/j.mocell.2025.100233

Seungjae Hwang, Jongsun Lee, Seung-Jae V. Lee

引用次数: 0

Advancing precision diagnosis in autism: Insights from large-scale genomic studies 推进自闭症的精确诊断：来自大规模基因组研究的见解。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-26 DOI: 10.1016/j.mocell.2025.100248

Soo-Whee Kim , Joon-Yong An

{"title":"Advancing precision diagnosis in autism: Insights from large-scale genomic studies","authors":"Soo-Whee Kim , Joon-Yong An","doi":"10.1016/j.mocell.2025.100248","DOIUrl":"10.1016/j.mocell.2025.100248","url":null,"abstract":"<div><div>Autism spectrum disorder (ASD) is a neurodevelopmental condition with a complex genetic basis. Large-scale whole-exome sequencing and whole-genome sequencing studies, with increasing sample sizes and improved ancestral diversity, have significantly advanced the discovery of ASD-associated genes. In addition to identifying coding variants, whole-genome sequencing has facilitated the detection of risk noncoding variants in regulatory elements such as enhancers, promoters, and untranslated regions, prompting experimental validation of their functional impact on neurodevelopment. A deeper understanding of ASD genetic liability has revealed the interplay between rare and common variants. Moreover, genetic liability varies by sex and phenotype profile, underscoring the complexity of ASD’s genetic architecture. While the clinical application of these genomic insights remains in early stages, progress has been made in gene-based therapeutic development, the interpretation of noncoding risk variants, and the use of polygenic score for risk stratification. In this review, we summarize key findings from large-scale genomic studies, explore the role of coding and noncoding variants in ASD, and discuss emerging opportunities for translating these discoveries into clinical practice.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100248"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pattern recognition receptors and inflammasome: Now and beyond 模式识别受体和炎性体：现在和未来。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-15 DOI: 10.1016/j.mocell.2025.100239

SuHyeon Oh , Young Ki Choi , SangJoon Lee

{"title":"Pattern recognition receptors and inflammasome: Now and beyond","authors":"SuHyeon Oh , Young Ki Choi , SangJoon Lee","doi":"10.1016/j.mocell.2025.100239","DOIUrl":"10.1016/j.mocell.2025.100239","url":null,"abstract":"<div><div>Pattern recognition receptors (PRRs) are fundamental to the innate immune system, functioning to detect and eliminate invading pathogens by inhibiting their replication and limiting host tissue damage. Through direct recognition of pathogen-associated molecular patterns and damage-associated molecular patterns, PRRs initiate inflammatory responses, including cytokine production, and modulate the adaptive immune response. Ligand binding activates downstream signaling pathways that promote pathogen clearance and drive inflammasome assembly. Accumulating evidence underscores the critical role of PRRs in sensing cellular damage and preserving homeostasis. Importantly, interactions within PRR networks facilitate the formation of multiple PRR-containing inflammasomes (PANoptosome), enabling coordinated inflammatory cell death under combined pathogen-associated molecular pattern and damage-associated molecular pattern stimulation. A comprehensive understanding of these interconnected signaling networks is essential for elucidating the regulation of innate immunity and its implications for disease pathogenesis, particularly in the context of infection and inflammation. This review provides a detailed overview of PRR-ligand recognition, downstream signaling mechanisms, and inflammasome activation, and discusses emerging insights into PRR regulation that hold promise for novel immunotherapeutic interventions.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100239"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-07-18 DOI: 10.1016/S1016-8478(25)00083-4

引用次数: 0

Dietary salt induces taste desensitization via receptor internalization in Drosophila in a sexually dimorphic manner 饮食盐诱导味觉脱敏通过受体内化在果蝇在两性二态的方式。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-18 DOI: 10.1016/j.mocell.2025.100242

Linni Jin , Chul Hoon Kim , Jeong Taeg Seo , Seok Jun Moon

{"title":"Dietary salt induces taste desensitization via receptor internalization in Drosophila in a sexually dimorphic manner","authors":"Linni Jin , Chul Hoon Kim , Jeong Taeg Seo , Seok Jun Moon","doi":"10.1016/j.mocell.2025.100242","DOIUrl":"10.1016/j.mocell.2025.100242","url":null,"abstract":"<div><div>Sodium homeostasis, which is critical for survival, includes mechanisms for regulating salt intake that integrate central neural pathways with the peripheral taste system. Although the central homeostatic mechanisms of salt appetite are well-studied, the mechanisms by which dietary salt modulates peripheral taste responses remain unclear. We found increased dietary salt reduces salt preference in <em>Drosophila</em> by desensitizing sweet gustatory receptor neurons independent of internal sodium levels. We observed a reversible suppression of salt-evoked neural responses following salt exposure accomplished via clathrin-mediated endocytosis in males and both clathrin- and C-terminal binding protein-dependent endocytosis in females. We also found reversing gustatory receptor neuron sexual identity switched the desensitization pattern, indicating cell-autonomous control of this sexual dimorphism. Moreover, C-terminal binding protein-mediated macropinocytosis in females also dampened sweet taste responses, revealing a sex- and modality-specific mechanism underlying sensory adaptation. These findings reveal dietary experience can affect feeding behavior by reprograming peripheral taste responses, clarifying the plasticity of nutrient sensing.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100242"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dysregulated RNA-binding proteins and alternative splicing: Emerging roles in autism spectrum disorder 失调的RNA结合蛋白和选择性剪接：在自闭症谱系障碍中的新作用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-03 DOI: 10.1016/j.mocell.2025.100237

Jiwon Jeong , Hee-Jeong Yoo , Joon-Yong An , Sunjoo Jeong

引用次数: 0