Molecules and Cells最新文献_第8页

Recent advances and applications of human lung alveolar organoids 人类肺泡组织细胞的最新进展和应用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-28 DOI: 10.1016/j.mocell.2024.100140

Sun Kyung Kim , Eunho Sung , Kyungtae Lim

引用次数: 0

Brief guide to RT-qPCR RT-qPCR 简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-28 DOI: 10.1016/j.mocell.2024.100141

Dajeong Bong, Jooyeon Sohn, Seung-Jae V. Lee

引用次数: 0

Epidermal stem cells: Interplay with the skin microenvironment during wound healing 表皮干细胞：伤口愈合过程中与皮肤微环境的相互作用

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-21 DOI: 10.1016/j.mocell.2024.100138

Yun Ha Hur

引用次数: 0

Genetic and epigenetic alterations in aging and rejuvenation of human 人类衰老和返老还童过程中的基因和表观遗传学变化。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-19 DOI: 10.1016/j.mocell.2024.100137

Kyunghyuk Park , Min Chul Jeon , Dakyung Lee , Jong-Il Kim , Sun-Wha Im

{"title":"Genetic and epigenetic alterations in aging and rejuvenation of human","authors":"Kyunghyuk Park , Min Chul Jeon , Dakyung Lee , Jong-Il Kim , Sun-Wha Im","doi":"10.1016/j.mocell.2024.100137","DOIUrl":"10.1016/j.mocell.2024.100137","url":null,"abstract":"<div><div>All the information essential for life is encoded within our genome and epigenome, which orchestrates diverse cellular states spatially and temporally. In particular, the epigenome interacts with internal and external stimuli, encoding and preserving cellular experiences, and it serves as the regulatory base of the transcriptome across diverse cell types. The emergence of single-cell transcriptomic and epigenomic data collection has revealed unique omics signatures in diverse tissues, highlighting cellular heterogeneity. Recent research has documented age-related epigenetic changes at the single-cell level, alongside the validation of cellular rejuvenation through partial reprogramming, which involves simultaneous epigenetic modifications. These dynamic shifts, primarily fueled by stem cell plasticity, have catalyzed significant interest and cross-disciplinary research endeavors. This review explores the genomic and epigenomic alterations with aging, elucidating their reciprocal interactions. Additionally, it seeks to discuss the evolving landscape of rejuvenation research, with a particular emphasis on dissecting stem cell behavior through the lens of single-cell analysis. Moreover, it proposes potential research methodologies for future studies.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100137"},"PeriodicalIF":3.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequencing CURLY LEAF-associated RNAs in Arabidopsis revealed prevalent intergenic RNAs from the nuclear mitochondrial sequence 对拟南芥 CURLY LEAF 相关 RNA 进行测序，发现了来自核线粒体序列的普遍基因间 RNA。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-18 DOI: 10.1016/j.mocell.2024.100131

Lei Gao , Shengben Li , Hyun Suh Chang , Yun Ju Kim

{"title":"Sequencing CURLY LEAF-associated RNAs in Arabidopsis revealed prevalent intergenic RNAs from the nuclear mitochondrial sequence","authors":"Lei Gao , Shengben Li , Hyun Suh Chang , Yun Ju Kim","doi":"10.1016/j.mocell.2024.100131","DOIUrl":"10.1016/j.mocell.2024.100131","url":null,"abstract":"<div><div>Polycomb group (PcG) proteins play key roles in development by repressing thousands of targets through histone modifications. However, how PcG is recruited to specific targets is poorly understood. In <em>Arabidopsis</em>, certain noncoding RNAs are necessary for recruiting the PcG protein CURLY LEAF (CLF) to its target sites. However, RNAs associated with CLF have not been analyzed on a genomic scale; thus, it is unknown whether long noncoding RNA (lncRNA)-mediated PcG recruitment is a widespread mechanism. Here, we systematically searched for CLF-associated RNAs by RNA immunoprecipitation followed by deep sequencing. We identified 1,299 genic and 138 intergenic regions that produced CLF-associated mRNAs and putative lncRNAs, respectively. The genes producing CLF-associated RNAs are depleted in PcG targets, carry active chromatin marks, and are highly expressed, suggesting that CLF may have a nonspecific or promiscuous RNA-binding affinity, similar to animal PcG proteins. Notably, a significant portion of the CLF-associated lncRNAs is derived from the nuclear mitochondrial sequence, which is extensively marked by H3K27me3. These findings indicate that, while CLF-RNA interactions are widespread, they may not always correlate with PcG target sites, highlighting the complexity of PcG recruitment mechanisms in <em>Arabidopsis</em>.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100131"},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is FAM19A5 an adipokine? Peripheral FAM19A5 in wild-type, FAM19A5 knockout, and LacZ knockin mice FAM19A5 是一种脂肪因子吗？野生型、FAM19A5 基因敲除和 LacZ 基因敲除小鼠的外周 FAM19A5。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-18 DOI: 10.1016/j.mocell.2024.100125

Hoyun Kwak , Eun-Ho Cho , Eun Bee Cho , Yoo-Na Lee , Anu Shahapal , Hyo Jeong Yong , Arfaxad Reyes-Alcaraz , Yongwoo Jeong , Yerim Lee , Minhyeok Lee , Nui Ha , Sitaek Oh , Jae Keun Lee , Won Suk Lee , Won Kyum Kim , Sangjin Yoo , Soon-Gu Kwon , Jong-Ik Hwang , Jae Young Seong

{"title":"Is FAM19A5 an adipokine? Peripheral FAM19A5 in wild-type, FAM19A5 knockout, and LacZ knockin mice","authors":"Hoyun Kwak , Eun-Ho Cho , Eun Bee Cho , Yoo-Na Lee , Anu Shahapal , Hyo Jeong Yong , Arfaxad Reyes-Alcaraz , Yongwoo Jeong , Yerim Lee , Minhyeok Lee , Nui Ha , Sitaek Oh , Jae Keun Lee , Won Suk Lee , Won Kyum Kim , Sangjin Yoo , Soon-Gu Kwon , Jong-Ik Hwang , Jae Young Seong","doi":"10.1016/j.mocell.2024.100125","DOIUrl":"10.1016/j.mocell.2024.100125","url":null,"abstract":"<div><div><em>FAM19A5</em> is a novel secretory protein expressed primarily in the brain. However, a recent study reported that <em>FAM19A5</em> is an adipocyte-derived adipokine that regulates vascular smooth muscle function through sphingosine-1-phosphate receptor 2 (S1PR2). In our study, we investigated <em>FAM19A5</em> transcript and protein levels in peripheral tissues, including adipose tissues, from wild-type, <em>FAM19A5</em> knockout, and <em>FAM19A5</em>-<em>LacZ</em> knockin mice. We found that the <em>FAM19A5</em> transcript levels in the central nervous system were much greater than those in any of the peripheral tissues, including adipose tissues. Furthermore, the <em>FAM19A5</em> protein levels in adipose and reproductive tissues were below detectable limits for Western blot analysis and enzyme-linked immunosorbent assay (ELISA). Additionally, we found that the <em>FAM19A5</em> protein did not interact with S1PR2 in terms of G-protein-mediated signal transduction, β-arrestin recruitment, or ligand-mediated internalization. Taken together, our findings revealed basal levels of <em>FAM19A5</em> transcripts and proteins in peripheral tissues, confirming its primary expression in the central nervous system and lack of significant interaction with S1PR2.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100125"},"PeriodicalIF":3.7,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A FMRFamide-like neuropeptide FLP-12 signaling regulates head locomotive behaviors in Caenorhabditis elegans 一种类似于FMRFamide的神经肽FLP-12信号调节秀丽隐杆线虫的头部运动行为。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-17 DOI: 10.1016/j.mocell.2024.100124

Do-Young Kim , Kyeong Min Moon , Woojung Heo , Eun Jo Du , Cheon-Gyu Park , Jihye Cho , Jeong-Hoon Hahm , Byung-Chang Suh , KyeongJin Kang , Kyuhyung Kim

{"title":"A FMRFamide-like neuropeptide FLP-12 signaling regulates head locomotive behaviors in Caenorhabditis elegans","authors":"Do-Young Kim , Kyeong Min Moon , Woojung Heo , Eun Jo Du , Cheon-Gyu Park , Jihye Cho , Jeong-Hoon Hahm , Byung-Chang Suh , KyeongJin Kang , Kyuhyung Kim","doi":"10.1016/j.mocell.2024.100124","DOIUrl":"10.1016/j.mocell.2024.100124","url":null,"abstract":"<div><div>Neuropeptides play a critical role in regulating behaviors across organisms, but the precise mechanisms by which neuropeptides orchestrate complex behavioral programs are not fully understood. Here, we show that the FMRFamide-like neuropeptide FLP-12 signaling from the SMB head motor neurons modulates head locomotive behaviors, including stomatal oscillation in <em>Caenorhabditis elegans</em>. <em>lim-4</em> mutants, in which the SMB neurons are not properly specified, exhibited various head and body locomotive defects, including stomatal oscillation. Chronic activation or inhibition of neuropeptidergic signaling in the SMB motor neurons resulted in a decrease or increase in stomatal oscillation, respectively. The <em>flp-12</em> neuropeptide gene is expressed and acts in the SMB neurons to regulate head and body locomotion, including stomatal oscillation. Moreover, the <em>frpr-8</em> G protein-couple receptor (GPCR) and <em>gpa-7</em> Gα genes are expressed in the AVD command interneurons to relay the FLP-12 signal to mediate stomatal oscillation. Finally, heterologous expression of FRPR-8 either <em>Xenopus</em> oocytes or HEK293T cells conferred FLP-12 induced responses. Taken together, these results indicate that the <em>C. elegans</em> FMRFamide neuropeptide FLP-12 acts as a modulator of stomatal oscillation via the FRPR-8 GPCR and the GPA-7 G-protein.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100124"},"PeriodicalIF":3.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nobel-winning microRNA, the micromaestro of gene silencing 诺贝尔奖得主 microRNA--基因沉默的微观世界

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-15 DOI: 10.1016/j.mocell.2024.100123

Young-Kook Kim , Jinju Han

引用次数: 0

Visualization of endogenous enhancer-promoter interactions in a single nucleus through chromatin labeling 通过染色质标记可视化单个细胞核中内源性增强子-启动子相互作用的实用方法。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-08 DOI: 10.1016/j.mocell.2024.100121

Gunhee Park , Won-Ki Cho

引用次数: 0

A concise guide to essential R packages for analyses of DNA, RNA, and proteins 用于分析 DNA、RNA 和蛋白质的基本 R 软件包简明指南》（A Concise Guide to Essential R Packages for Analyses of DNA, RNA, and Proteins）。