Molecules and Cells最新文献_第7页

Editorial Board Members/Copyright 编辑委员会成员/版权

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-08-20 DOI: 10.1016/S1016-8478(25)00093-7

引用次数: 0

N-Terminal deleted isoforms of E3 ligase RNF220 are ubiquitously expressed and required for mouse muscle differentiation E3连接酶RNF220 n端缺失异构体普遍表达，是小鼠肌肉分化所必需的。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-07-01 DOI: 10.1016/j.mocell.2025.100250

SeokGyeong Choi , Sojung Ha , Donald J. Wolfgeher , Jee Won Kim , Young-Hyun Go , Hyuk-Jin Cha , Gyu-Un Bae , Stephen J. Kron , Woo-Young Kim

{"title":"N-Terminal deleted isoforms of E3 ligase RNF220 are ubiquitously expressed and required for mouse muscle differentiation","authors":"SeokGyeong Choi , Sojung Ha , Donald J. Wolfgeher , Jee Won Kim , Young-Hyun Go , Hyuk-Jin Cha , Gyu-Un Bae , Stephen J. Kron , Woo-Young Kim","doi":"10.1016/j.mocell.2025.100250","DOIUrl":"10.1016/j.mocell.2025.100250","url":null,"abstract":"<div><div>Four isoform peptides of the novel E3 ligase ring finger protein 220 (RNF220) have been identified in humans. However, all of the previous studies have predominantly focused on isoform 1 (the full-length form), which consists of 566 amino acids. Here, we show that a shorter isoform, which is 308 amino acids lacking most of the N-terminus (human isoform 4; mouse isoform 3; ΔN-RNF220), is the predominant and ubiquitously expressed variant that warrants functional investigation. Both isoform 1 and ΔN-RNF220 are expressed in the brain; however, ΔN-RNF220 is the major isoform expressed in all other tissues in mice. Consistently, H3K4me3 ChIP-seq data from ENCODE reveal that the transcription start site for ΔN-RNF220 demonstrates broader and stronger activity across human tissues than that of isoform 1. ΔN-RNF220 produces 2 peptides (4a and 4b) through alternative translation initiation, with isoform 4b displaying distinct subcellular localization, subnuclear structures and interaction with a nuclear protein WDR5. Notably, during embryonic stem cell differentiation into neural stem cells, isoform 1 expression increases, whereas ΔN-RNF220 expression decreases. In murine myoblasts, ΔN-RNF220 is the sole expressed isoform and is required for MyoD and myogenin expression, as well as for muscle differentiation. Our findings highlight ΔN-RNF220 as the ubiquitously and highly expressed variant, likely playing a fundamental role across tissues while exhibiting functional differences from isoform 1. These results emphasize the critical importance of ΔN-RNF220 in future studies investigating the biological functions of RNF220.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100250"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifaceted role of serine hydroxymethyltransferase in health and disease 丝氨酸羟甲基转移酶在健康和疾病中的多方面作用。

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-07-28 DOI: 10.1016/j.mocell.2025.100262

Jing Zhang , Seong Eun Lee , Jiyeon Yoon , Bon Jeong Ku , Junyoung O. Park , Da Hyun Kang , Jun Young Heo , Yea Eun Kang

{"title":"Multifaceted role of serine hydroxymethyltransferase in health and disease","authors":"Jing Zhang , Seong Eun Lee , Jiyeon Yoon , Bon Jeong Ku , Junyoung O. Park , Da Hyun Kang , Jun Young Heo , Yea Eun Kang","doi":"10.1016/j.mocell.2025.100262","DOIUrl":"10.1016/j.mocell.2025.100262","url":null,"abstract":"<div><div>Serine hydroxymethyltransferase (SHMT) is a key enzyme in 1-carbon metabolism, a biochemical pathway critical for cellular growth, proliferation, and survival. One-carbon metabolism integrates the folate and methionine cycles to produce essential intermediates necessary for nucleotide synthesis, methylation reactions, and redox homeostasis. SHMT exists in 2 isoforms, SHMT1, which is localized in the cytoplasm, and SHMT2, which is localized in the mitochondria. SHMT1 and SHMT2 have distinct yet complementary functions. Both are involved in serine and glycine metabolism, ensuring a continuous supply of the 1-carbon units required for biosynthetic and epigenetic processes. SHMT dysregulation has been implicated in cancer progression and metabolic disorders, including cardiovascular diseases, diabetes, and neurological abnormalities. In cancer, the abnormal expression of SHMT has been associated with tumor growth, metabolic reprogramming, and treatment resistance, and has also been shown to correlate with poor patient outcomes. Considering its critical role in both cancer and metabolic diseases, SHMT has emerged as a potential therapeutic target in cancer. Recent studies have shown that SHMT inhibitors can reduce tumor proliferation and restore metabolic homeostasis. This review provides a comprehensive overview of the role of SHMT in the regulation of metabolic pathways and its role in tumor progression and metabolic diseases. In this review, we aimed to highlight the therapeutic potential of targeting SHMT and offer insights into the development of innovative treatment strategies in oncology and metabolic medicine. These insights support the hypothesis that targeting SHMT, particularly isoform-specific inhibition, may provide novel therapeutic avenues in both oncology and metabolic medicine.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100262"},"PeriodicalIF":6.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brief guide to Caenorhabditis elegans imaging and quantification 秀丽隐杆线虫成像与定量简介。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-06-29 DOI: 10.1016/j.mocell.2025.100249

Hyemin Min, Gyutae Park, Seung-Jae V. Lee

引用次数: 0

Cyclotides as novel plant-derived scaffolds for orally active cyclic peptide therapeutics 环肽作为口服活性环肽治疗的新型植物源支架。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-07-04 DOI: 10.1016/j.mocell.2025.100252

Youbong Hyun

{"title":"Cyclotides as novel plant-derived scaffolds for orally active cyclic peptide therapeutics","authors":"Youbong Hyun","doi":"10.1016/j.mocell.2025.100252","DOIUrl":"10.1016/j.mocell.2025.100252","url":null,"abstract":"<div><div>Cyclotides are a group of plant-derived small peptides, characterized by a head-to-tail cyclic backbone and 3 conserved cysteine knots. The unique structure endows cyclotides with exceptional chemical stability and intrinsic cell-penetrating capacities, allowing them to serve as orally active agents for host defense. These properties also position cyclotides as ideal scaffolds for the design of novel edible drugs. This review provides a comprehensive overview of the discovery, distribution, and evolutionary divergence of cyclotides in flowering plants, while also discussing their classification and applications in structure-based engineering for various purposes. Additionally, recent advancements in the biological and pharmaceutical activities of both naturally occurring and synthetic “designer” cyclotides are summarized. The complexities of the cyclotide biosynthetic pathway have posed challenges for their synthesis in non-native organisms. Thus, the article concludes by exploring the latest strategies for large-scale cyclotide production in plants, highlighting <em>Viola</em> species as rich sources for future cyclotide engineering.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100252"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover and caption 封面及标题

IF 6.5 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-08-20 DOI: 10.1016/S1016-8478(25)00091-3

引用次数: 0

Tumor-derived CD84 promotes growth of acute myeloid leukemia cells via regulating nonhomologous DNA end-joining pathway 肿瘤源性CD84通过调节非同源DNA末端连接途径促进急性髓系白血病细胞生长。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-09-01 Epub Date: 2025-07-07 DOI: 10.1016/j.mocell.2025.100253

Xiang Li , Bo Chen , Jiajing Zhou , Chunling Li , Yue Yu , Erdi Zhao , Xingli Wu , Changzhu Jin , Minjing Li , Jiankai Feng , Leilei Lin , Yancun Yin

{"title":"Tumor-derived CD84 promotes growth of acute myeloid leukemia cells via regulating nonhomologous DNA end-joining pathway","authors":"Xiang Li , Bo Chen , Jiajing Zhou , Chunling Li , Yue Yu , Erdi Zhao , Xingli Wu , Changzhu Jin , Minjing Li , Jiankai Feng , Leilei Lin , Yancun Yin","doi":"10.1016/j.mocell.2025.100253","DOIUrl":"10.1016/j.mocell.2025.100253","url":null,"abstract":"<div><div>CD84, a member of the signaling lymphocyte activation molecule immunoglobulin superfamily, has been identified as playing a significant role in regulating various immune cell activities. However, its intrinsic role in cancer cells remains largely unknown. We aim to explore the direct role of CD84 in acute myeloid leukemia (AML) progression and to clarify the underlying molecular mechanisms involved in nonhomologous end-joining (NHEJ) repair. Herein, we found that CD84 is frequently upregulated in various types of AML and leukemia initiation cells (LICs)-enriched cells. Knockdown or blocking of CD84 significantly inhibits the growth and induces the apoptosis of AML cells. Moreover, knockdown of <em>CD84</em> significantly delays AML progression and prolongs the survival of the xenografted mice in vivo. Mechanistically, CD84 promotes the expression of NHEJ core factors by recruiting SAP and activating the AKT signaling pathway. Knockdown of <em>CD84</em> inhibits NHEJ repair in AML cells via regulating the expression of NHEJ core factors, including <em>PRKDC</em>, <em>LIG4</em>, <em>XRCC5</em>, and <em>DCLRE1C.</em> Subsequently, this leads to double-strand breaks accumulation and cell apoptosis. Importantly, CD84 is required for the proliferation and self-renewal of human LICs. In conclusion, CD84 plays important roles in AML growth and progression through promoting NHEJ repair. Targeting CD84 may be a potential approach for inhibiting AML development and eliminating LICs.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 9","pages":"Article 100253"},"PeriodicalIF":3.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144601023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Building blocks for lung regeneration: Stem cells and niches 肺再生的构建模块：干细胞和壁龛。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-13 DOI: 10.1016/j.mocell.2025.100241

Hyeyoung Lee , Chae Won Lim , Woo Ram Lee , Ji Eun Park , Da Yeon Yu , Jinwook Choi

{"title":"Building blocks for lung regeneration: Stem cells and niches","authors":"Hyeyoung Lee , Chae Won Lim , Woo Ram Lee , Ji Eun Park , Da Yeon Yu , Jinwook Choi","doi":"10.1016/j.mocell.2025.100241","DOIUrl":"10.1016/j.mocell.2025.100241","url":null,"abstract":"<div><div>The respiratory system is an essential organ that performs gas exchange through the blood circulation in mammals. Unlike other organs, the lungs are directly exposed to the external environment, including particulate matter, cigarette smoke, and various pollutants, and are therefore, highly susceptible to damage. The lungs retain regional-specific stem/progenitor cells that quickly mobilize to replace the damaged epithelium. Accumulating evidences suggest that fate decision of stem cells relies on regulatory programs integrated by niches constituting the microenvironment providing diverse signals that regulate stem cell behavior. Therefore, understanding cellular diversity and precise interaction between stem cells and their respective niches is crucial to understand how tissue recovers homeostasis after injury. Here, in this review, we summarize recent progress in cellular and functional identity of stem cells and distinctive niches in the lungs. We also describe the molecular mechanism of genetic and epigenetic program in the regulation of stem cell behavior during tissue regeneration. Lastly, we introduce the three-dimensional lung organoid platforms that provide valuable insights into the mechanisms of lung pathophysiology in human system.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100241"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

READRetro web: A user-friendly platform for predicting plant natural product biosynthesis READRetro Web：一个预测植物天然产物生物合成的用户友好平台。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-06-02 DOI: 10.1016/j.mocell.2025.100235

Yejin Kwak , Taein Kim , Sang-Gyu Kim , Jeongbin Park

{"title":"READRetro web: A user-friendly platform for predicting plant natural product biosynthesis","authors":"Yejin Kwak , Taein Kim , Sang-Gyu Kim , Jeongbin Park","doi":"10.1016/j.mocell.2025.100235","DOIUrl":"10.1016/j.mocell.2025.100235","url":null,"abstract":"<div><div>Natural products (NPs), a fundamental class of bioactive molecules with broad applicability, are valuable sources in pharmaceutical research and drug discovery. Despite their significance, the large-scale production of NPs is often limited by their availability and scalability, requiring alternative approaches such as metabolic engineering or biosynthesis. To identify ideal pathways for the mass production of NPs, deep learning-based retrosynthesis models have been recently developed. Such models accelerate NP discovery; however, these tools are often not easy to use for researchers with a limited computational background, because they require complex environment configurations, command-line interfaces, and substantial computational resources. Here, we introduce READRetro web, a user-friendly web platform that integrates the READRetro machine learning (ML) model for retrosynthesis prediction. Based on modern web technologies, our web platform provides a fast and responsive user experience. READRetro Web bridges the gap between advanced ML-driven retrosynthesis and practical research workflows, making retrosynthesis prediction accessible to a broader range of researchers. Our platform demonstrates high predictive accuracy and computational efficiency, offering well-organized results to facilitate NP retrosynthetic pathway design. READRetro Web is freely accessible via <span><span>https://readretro.net</span><svg><path></path></svg></span>.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"48 8","pages":"Article 100235"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover and caption 封面及标题

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2025-08-01 Epub Date: 2025-07-18 DOI: 10.1016/S1016-8478(25)00082-2

引用次数: 0