Molecules and Cells最新文献_第7页

Cellular and metabolic function of GIRK1 potassium channels expressed by arcuate POMC and NPY/AgRP neurons 弓状 POMC 和 NPY/AgRP 神经元表达的 GIRK1 钾通道的细胞和代谢功能。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100122

Yeeun Choi, Eun-Seon Yoo, Youjin Oh, Jong-Woo Sohn

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A brief guide to analyzing expression quantitative trait loci 表达量性状位点分析简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100139

Byung Su Ko , Sung Bae Lee , Tae-Kyung Kim

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The emerging role of gut hormones 肠道激素的新作用

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100126

Hyeryeong Cho , Jaechul Lim

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Transcriptome-wide analysis for glucocorticoid receptor-mediated mRNA decay reveals various classes of target transcripts 对糖皮质激素受体介导的 mRNA 衰减进行的全转录组分析揭示了各类目标转录本。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100130

Sung Ho Boo , Min-Kyung Shin , Hongseok Ha , Jae-Sung Woo , Yoon Ki Kim

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The role of p21 in cellular senescence and aging-related diseases p21 在细胞衰老和衰老相关疾病中的作用。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100113

Jiayu Yan, Siyi Chen, Zimei Yi, Ruowen Zhao, Jiayu Zhu, Shuwen Ding, Junhua Wu

{"title":"The role of p21 in cellular senescence and aging-related diseases","authors":"Jiayu Yan, Siyi Chen, Zimei Yi, Ruowen Zhao, Jiayu Zhu, Shuwen Ding, Junhua Wu","doi":"10.1016/j.mocell.2024.100113","DOIUrl":"10.1016/j.mocell.2024.100113","url":null,"abstract":"<div><div>During the aging process or disease progression, normal cells and tissues in the body undergo various stresses, leading to cell damage and the need for repair, adaptation, apoptosis, or defense responses. Cellular senescence is a key player in this process, influencing the rate of aging and disease progression. It can be triggered by different stress factors, resulting in irreversible cell cycle arrest and functional decline. Senescent cells often show high expression of cell cycle factors such as p21 and p16, which are involved in cell cycle arrest. p16 has long been recognized as a significant marker of aging. Recent evidence suggests that p21<sup>high</sup> cells and p16<sup>high</sup> cells represent distinct cell populations in terms of cell type, tissue location, accumulation kinetics, and physiological functions. This article focuses on recent advancements in understanding p21-dependent cellular senescence. It starts by providing an overview of the role of p21 in 3 primary cellular senescence phenotypes where it plays a crucial role. It then delves into the pathogenesis of diseases closely linked to p21-dependent cellular senescence, particularly metabolic disorders and cardiovascular diseases. The article also discusses progress in p21-related animal models and outlines strategies for utilizing p21 to intervene in cellular senescence by delaying aging, eliminating senescent cells, and rejuvenating senescent cells. This review systematically examines the pathogenesis of p21-dependent cellular senescence, emphasizing its importance in studying aging heterogeneity and developing new senolytic therapies. It aims to stimulate future research on leveraging p21 to enhance the characteristics of senescent cells, allowing more precise methods for eliminating harmful senescent cells at the right time, thereby delaying aging and potentially achieving rejuvenation.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 11","pages":"Article 100113"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of phospholipase D1-inhibitory peptide on the growth and metastasis of gastric cancer cells 磷脂酶 D1 抑制肽对胃癌细胞生长和转移的影响

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100128

Dongju Kim , Mee-Sup Yoon , Junwon Lee , Shin-Young Park , Joong-Soo Han

{"title":"Effects of phospholipase D1-inhibitory peptide on the growth and metastasis of gastric cancer cells","authors":"Dongju Kim , Mee-Sup Yoon , Junwon Lee , Shin-Young Park , Joong-Soo Han","doi":"10.1016/j.mocell.2024.100128","DOIUrl":"10.1016/j.mocell.2024.100128","url":null,"abstract":"<div><div>Phospholipase D1 (PLD1) contributes to cancer development and progression through its effects on cell proliferation, survival, invasion, metastasis, angiogenesis, drug resistance, and modulation of the tumor microenvironment. Its central role in these processes makes it a promising target for novel cancer treatments aimed at inhibiting its activity and disrupting the signaling pathways it regulates. In this study, we aimed to investigate the effect of PLD1 inhibition on gastric cancer cell growth using a novel peptide inhibitor, TAT-TVTSP. PLD1, which plays a role in cancer progression, catalyzes the conversion of phosphatidylcholine into choline and phosphatidic acid through hydrolysis. To effectively target PLD1 in cells, we engineered TAT-TVTSP by fusing a PLD1-inhibitory peptide (TVTSP) with a cell-penetrating peptide (TAT). We observed that TAT-TVTSP effectively inhibited PLD1 activity in AGS gastric cancer cells. Moreover, TAT-TVTSP significantly inhibited the mammalian target of the rapamycin signaling pathway, including the phosphorylation of key downstream targets such as S6K1, AKT, S473, glycogen synthase kinase-3b, and forkhead box O1. TAT-TVTSP did not induce cell death, but it triggered cell cycle arrest by activating p21 and p27 via AKT phosphorylation. Functional assays revealed that TAT-TVTSP significantly impaired the colony-forming ability of AGS cells, thus inhibiting cell proliferation. Transwell and wound-healing assays revealed that this peptide disrupted the cellular behaviors critical to cancer progression, such as migration and invasion. In vivo, TAT-TVTSP significantly reduced tumor growth in the xenograft model of gastric cancer without any toxicity. Overall, our results suggest that TAT-TVTSP is a novel therapeutic agent for PLD1-mediated cancers.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 11","pages":"Article 100128"},"PeriodicalIF":3.7,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of organ developmental toxicity of environmental toxicants using zebrafish embryos 利用斑马鱼胚胎评估环境毒物对器官发育的毒性。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100144

Taeyeon Hong , Junho Park , Garam An , Jisoo Song , Gwonhwa Song , Whasun Lim

引用次数: 0

Brief guide to flow cytometry 流式细胞仪简要指南。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-11-01 DOI: 10.1016/j.mocell.2024.100129

Youngkwon Song, Yoontae Lee

引用次数: 0

Unveiling the antimetastatic activity of monoterpene indole alkaloids targeting MMP9 in cancer cells, with a focus on pharmacokinetic and cellular insights 揭示以 MMP9 为靶点的单萜吲哚生物碱在癌细胞中的抗转移活性，重点是药代动力学和细胞学方面的见解。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-29 DOI: 10.1016/j.mocell.2024.100143

Mücahit Varlı , Kyungha Lee , Kyo Bin Kang , Hangun Kim

{"title":"Unveiling the antimetastatic activity of monoterpene indole alkaloids targeting MMP9 in cancer cells, with a focus on pharmacokinetic and cellular insights","authors":"Mücahit Varlı , Kyungha Lee , Kyo Bin Kang , Hangun Kim","doi":"10.1016/j.mocell.2024.100143","DOIUrl":"10.1016/j.mocell.2024.100143","url":null,"abstract":"<div><div>Distant metastasis, together with acquired resistance, limits the therapeutic impact of chemotherapy and molecularly targeted therapies. The properties of the tumor microenvironment determine how sensitive or resistant various cancers are to specific pharmacological treatments. Matrix metalloproteinase 9 (MMP9) is widely known for its ability to break down the extracellular matrix and it also modulates the motility of cancer cells. Here, our goal was to identify compounds that target MMP9 and evaluate their capacity to inhibit the motility of cancer cells. The antimetastatic effect of monoterpene indole alkaloids (MIAs) on cell viability and motility was evaluated by methyl thiazolyl tetrazolium assay, migration assay, invasion assay, quantitative real-time polymerase chain reaction, pathway-focused expression analysis, Western blotting, reporter assay, molecular docking simulation, and target prediction. MIA compounds target MMP9. MIAs inhibited the expression of phospho-epidermal growth factor receptor, phospho-Akt, phospho-JNK, and cyclin D1. Additionally, MIAs had predicted favorable pharmacokinetic profile and drug-like properties. Furthermore, among the MIA compounds, lyaloside and 5(S)-5-carbomethoxystrictosidine had low cytotoxicity and regulated cancer-related signaling, including cell migration, cell invasion, epithelial-mesenchymal transition, and immune evasion. Our findings demonstrated that the MIAs used in this study have potential antimetastasis properties that occur via MMP9-mediated regulation of cancer signaling and have the potential to be used therapeutically at safe doses.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100143"},"PeriodicalIF":3.7,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142558275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transport of Golgi-localized β-catenin p-S47 by KIF11 or KIFC3 induces primary ciliogenesis KIF11或KIFC3运输高尔基定位的β-catenin p-S47可诱导原发性纤毛虫的发生。

IF 3.7 3区生物学

Molecules and Cells Pub Date : 2024-10-28 DOI: 10.1016/j.mocell.2024.100142

Na Yeong Lee , Mi-Lang Kyun , Ji Eun Yu , Sun-Ok Kim , Key-Hwan Lim , Kyung Ho Lee

{"title":"Transport of Golgi-localized β-catenin p-S47 by KIF11 or KIFC3 induces primary ciliogenesis","authors":"Na Yeong Lee , Mi-Lang Kyun , Ji Eun Yu , Sun-Ok Kim , Key-Hwan Lim , Kyung Ho Lee","doi":"10.1016/j.mocell.2024.100142","DOIUrl":"10.1016/j.mocell.2024.100142","url":null,"abstract":"<div><div>Primary cilium is an important hub for cell signaling and dysregulation of primary cilia assembly and disassembly is associated with the development of cancer and chemotherapeutic drug resistance, as well as the genetic disorders collectively known as ciliopathy. β-catenin plays a major role in canonical Wnt signaling; however, its association with primary cilia has only recently been highlighted in reports of β-catenin-mediated primary ciliogenesis. In this study, we found that β-catenin p-S47 was localized to the Golgi apparatus and the nucleus, and the amount of β-catenin p-S47 at these locations was significantly higher during primary ciliogenesis compared with asynchronous cell growth conditions. In addition, the novel β-catenin-binding motor proteins KIF11 and KIFC3 were shown to have a lower binding affinity in β-catenin S47A than in β-catenin wild-type. Knockdown of KIF11 or KIFC3 resulted in primary cilia deficiency and increased β-catenin p-S47 levels in the Golgi apparatus and were accompanied by a decrease in β-catenin p-S47 at the centrosome. The accumulation of β-catenin p-S47 in the nucleus was increased during primary ciliogenesis along with β-catenin-dependent transcriptional activity. The collective findings indicate the existence of a novel mechanism of primary ciliogenesis involving KIF11-/KIFC3-associated β-catenin p-S47 in the Golgi apparatus and β-catenin p-S47 transcriptional activity in the nucleus. This study revealed a new mechanism for the study of ciliopathies, cancer, and chemotherapeutic drug resistance caused by primary ciliogenesis dysregulation and provides new targets for drug development to treat these diseases.</div></div>","PeriodicalId":18795,"journal":{"name":"Molecules and Cells","volume":"47 12","pages":"Article 100142"},"PeriodicalIF":3.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0