NanoImpact最新文献

{"title":"Environmental and health impacts of functional graphenic materials and their ultrasonically altered products","authors":"Walker M. Vickery ,&nbsp;Hunter B. Wood ,&nbsp;Jason D. Orlando ,&nbsp;Juhi Singh ,&nbsp;Chenyun Deng ,&nbsp;Li Li ,&nbsp;Jing-Yi Zhou ,&nbsp;Frederick Lanni ,&nbsp;Aidan W. Porter ,&nbsp;Stefanie A. Sydlik","doi":"10.1016/j.impact.2023.100471","DOIUrl":"10.1016/j.impact.2023.100471","url":null,"abstract":"<div><p>Graphenic materials have excited the scientific community due to their exciting mechanical, thermal, and optoelectronic properties for a potential range of applications. Graphene and graphene derivatives have demonstrated application in areas stretching from composites to medicine; however, the environmental and health impacts of these materials have not been sufficiently characterized. Graphene oxide (GO) is one of the most widely used graphenic derivatives due to a relatively easy and scalable synthesis, and the ability to tailor the oxygen containing functional groups through further chemical modification. In this paper, ecological and health impacts of fresh and ultrasonically altered functional graphenic materials (FGMs) were investigated. Model organisms, specifically <em>Escherichia coli</em>, <em>Bacillus subtilis</em>, and <em>Caenorhabditis elegans</em>, were used to assess the consequences of environmental exposure to fresh and ultrasonically altered FGMs. FGMs were selected to evaluate the environmental effects of aggregation state, degree of oxidation, charge, and ultrasonication. The major findings indicate that bacterial cell viability, nematode fertility, and nematode movement were largely unaffected, suggesting that a wide variety of FGMs may not pose significant health and environmental risks.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100471"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10140101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opportunities for the use of selenium nanoparticles in agriculture","authors":"Jiangyun Song ,&nbsp;Sujuan Yu ,&nbsp;Rui Yang ,&nbsp;Junping Xiao ,&nbsp;Jingfu Liu","doi":"10.1016/j.impact.2023.100478","DOIUrl":"10.1016/j.impact.2023.100478","url":null,"abstract":"<div><p>Due to the growing number of the world's population, there is an urgent need for high-quality food to meet global food security. Traditional fertilizers and pesticides face the problems of low utilization efficiency and possible hazards to non-target organisms. Selenium (Se) is an essential trace element for animals and humans. As a result, Se nanoparticles (SeNPs) have aroused intense interest and found opportunities in agricultural use. Herein, we summarized representative studies on the potential application of SeNPs in agriculture, including mitigating biotic and abiotic stresses in plants, promoting seed germination and plant growth, and improving Se contents and nutritional values in crops, and the underlying mechanisms were also discussed. Finally, future directions are highlighted to get a deep insight into this field.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100478"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10191605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation, behavior, properties and impact of micro- and nanoplastics on agricultural soil ecosystems (A Review)","authors":"Anton F. Astner ,&nbsp;Alexis B. Gillmore ,&nbsp;Yingxue Yu ,&nbsp;Markus Flury ,&nbsp;Jennifer M. DeBruyn ,&nbsp;Sean M. Schaeffer ,&nbsp;Douglas G. Hayes","doi":"10.1016/j.impact.2023.100474","DOIUrl":"10.1016/j.impact.2023.100474","url":null,"abstract":"<div><p>Micro and nanoplastics (MPs and NPs, respectively) in agricultural soil ecosystems represent a pervasive global environmental concern, posing risks to soil biota, hence soil health and food security. This review provides a comprehensive and current summary of the literature on sources and properties of MNPs in agricultural ecosystems, methodology for the isolation and characterization of MNPs recovered from soil, MNP surrogate materials that mimic the size and properties of soil-borne MNPs, and transport of MNPs through the soil matrix. Furthermore, this review elucidates the impacts and risks of agricultural MNPs on crops and soil microorganisms and fauna. A significant source of MPs in soil is plasticulture, involving the use of mulch films and other plastic-based implements to provide several agronomic benefits for specialty crop production, while other sources of MPs include irrigation water and fertilizer. Long-term studies are needed to address current knowledge gaps of formation, soil surface and subsurface transport, and environmental impacts of MNPs, including for MNPs derived from biodegradable mulch films, which, although ultimately undergoing complete mineralization, will reside in soil for several months. Because of the complexity and variability of agricultural soil ecosystems and the difficulty in recovering MNPs from soil, a deeper understanding is needed for the fundamental relationships between MPs, NPs, soil biota and microbiota, including ecotoxicological effects of MNPs on earthworms, soil-dwelling invertebrates, and beneficial soil microorganisms, and soil geochemical attributes. In addition, the geometry, size distribution, fundamental and chemical properties, and concentration of MNPs contained in soils are required to develop surrogate MNP reference materials that can be used across laboratories for conducting fundamental laboratory studies.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100474"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10132532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A data reusability assessment in the nanosafety domain based on the NSDRA framework followed by an exploratory quantitative structure activity relationships (QSAR) modeling targeting cellular viability","authors":"Irini Furxhi ,&nbsp;Egon Willighagen ,&nbsp;Chris Evelo ,&nbsp;Anna Costa ,&nbsp;Davide Gardini ,&nbsp;Ammar Ammar","doi":"10.1016/j.impact.2023.100475","DOIUrl":"10.1016/j.impact.2023.100475","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Introduction&lt;/h3&gt;&lt;p&gt;The current effort towards the digital transformation across multiple scientific domains requires data that is Findable, Accessible, Interoperable and Reusable (FAIR). In addition to the FAIR data, what is required for the application of computational tools, such as Quantitative Structure Activity Relationships (QSARs), is a sufficient data volume and the ability to merge sources into homogeneous digital assets. In the nanosafety domain there is a lack of FAIR available metadata.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methodology&lt;/h3&gt;&lt;p&gt;To address this challenge, we utilized 34 datasets from the nanosafety domain by exploiting the NanoSafety Data Reusability Assessment (NSDRA) framework, which allowed the annotation and assessment of dataset's reusability. From the framework's application results, eight datasets targeting the same endpoint (i.e. numerical cellular viability) were selected, processed and merged to test several hypothesis including universal versus nanogroup-specific QSAR models (metal oxide and nanotubes), and regression versus classification Machine Learning (ML) algorithms.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Universal regression and classification QSARs reached an 0.86 R&lt;sup&gt;2&lt;/sup&gt; and 0.92 accuracy, respectively, for the test set. Nanogroup-specific regression models reached 0.88 R&lt;sup&gt;2&lt;/sup&gt; for nanotubes test set followed by metal oxide (0.78). Nanogroup-specific classification models reached 0.99 accuracy for nanotubes test set, followed by metal oxide (0.91). Feature importance revealed different patterns depending on the dataset with common influential features including core size, exposure conditions and toxicological assay.&lt;/p&gt;&lt;p&gt;Even in the case where the available experimental knowledge was merged, the models still failed to correctly predict the outputs of an unseen dataset, revealing the cumbersome conundrum of scientific reproducibility in realistic applications of QSAR for nanosafety. To harness the full potential of computational tools and ensure their long-term applications, embracing FAIR data practices is imperative in driving the development of responsible QSAR models.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;p&gt;This study reveals that the digitalization of nanosafety knowledge in a reproducible manner has a long way towards its successful pragmatic implementation. The workflow carried out in the study shows a promising approach to increase the FAIRness across all the elements of computational studies, from dataset's annotation, selection, merging to FAIR modeling reporting. This has significant implications for future research as it provides an example of how to utilize and report different tools available in the nanosafety knowledge system, while increasing the transparency of the results. One of the main benefits of this workflow is that it promotes data sharing and reuse, which is essential for advancing scientific knowledge by making data and metadata FAIR compliant. In addition, the increased transparen","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100475"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10133551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon nanomaterials are a superior soil amendment for sandy soils than biochar based on impacts on lettuce growth, physiology and soil biochemical quality","authors":"Jaya Nepal ,&nbsp;Xiaoping Xin ,&nbsp;Gabriel Maltais-Landry ,&nbsp;Wiqar Ahmad ,&nbsp;Jorge Pereira ,&nbsp;Swadeshmukul Santra ,&nbsp;Alan L. Wright ,&nbsp;Andy Ogram ,&nbsp;Peter J. Stofella ,&nbsp;Zhenli He","doi":"10.1016/j.impact.2023.100480","DOIUrl":"10.1016/j.impact.2023.100480","url":null,"abstract":"<div><p>A significant bottleneck of current agricultural systems remains the very low agronomic efficiency of conventional agrochemicals, particularly in sandy soils. Carbon nanomaterials (CNMs) have been proposed to address this inefficiency in sandy soils, which could potentially improve soil fertility and enhance crop growth and physiological processes. However, the effects of different rates of CNMs on crop physiological and soil biochemical quality in sandy soils must be compared to other carbon sources (e.g., biochar) before CNMs can be broadly used. To address this, a 70-day pot experiment was set up, growing lettuce under ten treatments: a negative control with no CNMs, biochar or fertilizer; a fertilizer-only control; three CNMs-only unfertilized treatments (CNMs at 200, 400 and 800 mg kg⁻¹ soil); two biochar treatments with fertilizer (biochar at 0.5% and 1% by soil mass + fertilizer); and three CNMs treatments with fertilizer (CNMs at 200, 400 and 800 mg kg⁻¹ soil + fertilizer). A novel amorphous, water-dispersible, and carboxyl-functionalized CNMs with pH of 5.5, zeta potential of −40.6 mV and primary particle diameter of 30–60 nm was used for this experiment. Compared to the fertilizer-only control, CNMs applied at low to medium levels (200–400 mg kg⁻¹) significantly increased lettuce shoot biomass (20–21%), total chlorophyll (23–27%), and fluorescence and photosynthetic activities (4–10%), which was associated with greater soil nutrient availability (N: 24–58%, K: 68–111%) and higher leaf tissue accumulation (N: 25–27%; K: 66%). Low to medium levels of CNMs also significantly increased soil biochemical properties, such as higher soil microbial biomass carbon (27–29%) and urease enzyme activity (34–44%) relative to fertilizer-only applications. In contrast, biochar (0.5%) increased lettuce biomass relative to fertilizer-only but had no significant effect on soil fertility and biological properties. These results suggest that CNMs at low to medium application rates are a superior carbon-based amendment relative to biochar in sandy soils.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100480"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological evaluation of therapeutically active zinc oxide nanoflowers in pre-clinical mouse model","authors":"Ayan Kumar Barui ,&nbsp;Vishnu Sravan Bollu ,&nbsp;Swapnali Londhe ,&nbsp;Shruti S. Deshpande ,&nbsp;Sourav Das ,&nbsp;Susheel Kumar Nethi ,&nbsp;Muntadher Mazin Abdulkareem Alabbasi ,&nbsp;Madhusudana Kuncha ,&nbsp;Jerald Mahesh Kumar ,&nbsp;Ramakrishna Sistla ,&nbsp;Sunil Misra ,&nbsp;Chitta Ranjan Patra","doi":"10.1016/j.impact.2023.100479","DOIUrl":"10.1016/j.impact.2023.100479","url":null,"abstract":"<div><p>Our earlier reports established that zinc oxide nanoflowers (ZONF) show significant pro-angiogenic properties, where reactive oxygen species, nitric oxide and MAPK-AKT-eNOS cell signaling axis play an essential task. Considering the significance of angiogenesis in healthcare, our research group has recently demonstrated the <em>in vivo</em> therapeutic application of ZONF (10 mg/kg b.w.) for treating peripheral artery disease. Moreover, based on the angio-neural crosstalk between vascular and neuronal systems, we have further demonstrated the neuritogenic and neuroprotective characteristics of pro-angiogenic nanoflowers (10 mg/kg b.w.) for the treatment of cerebral ischemia. However, it is crucial for a therapeutic material to be non-toxic for its practical clinical applications and therefore assessment of its <em>in vivo</em> toxicity and adverse effect is highly important. Herein, for the first time, we investigate a detailed nanotoxicology of therapeutically active ZONF in Swiss albino mice to evaluate their safety profile and comprehend their aspects for future clinical applications. The maximum tolerated dose (MTD) of ZONF was found to be 512.5 mg/kg b.w. which was employed for acute exposure (2 weeks), showing slight toxicity. However, sub-chronic (4 weeks) and long term chronic (8–12 weeks) studies of nanoflowers exhibited their non-toxic nature particularly at lower therapeutic doses (1–10 mg/kg b.w.). Additionally, in depth genotoxicity study revealed that lower therapeutic dose of ZONF (10 mg/kg b.w.) did not exhibit significant toxicity even in genetic level. Overall, the present nanotoxicology of ZONF suggests their high biocompatible nature at therapeutic dose, offering the basis of their future clinical applications in ischemic and other vascular diseases.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100479"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10144682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional response of Cu-deficient barley (Hordeum vulgare L.) to foliar-applied nano-Cu: Molecular crosstalk between Cu loading into plants and changes in Cu homeostasis genes","authors":"Magdalena Kusiak ,&nbsp;Magdalena Sozoniuk ,&nbsp;Camille Larue ,&nbsp;Renato Grillo ,&nbsp;Krzysztof Kowalczyk ,&nbsp;Patryk Oleszczuk ,&nbsp;Izabela Jośko","doi":"10.1016/j.impact.2023.100472","DOIUrl":"10.1016/j.impact.2023.100472","url":null,"abstract":"<div><p>For safe and effective nutrient management, the cutting-edge approaches to plant fertilization are continuously developed. The aim of the study was to analyze the transcriptional response of barley suffering from Cu deficiency to foliar application of nanoparticulate Cu (nano-Cu) and its ionic form (CuSO₄) at 100 and 1000 mg L⁻¹ for the examination of their supplementing effect. The initial interactions of Cu-compounds with barley leaves were analyzed with spectroscopic (ICP-OES) and microscopic (SEM-EDS) methods. To determine Cu cellular status, the impact of Cu-compounds on the expression of genes involved in regulating Cu homeostasis (<em>PAA1</em>, <em>PAA2</em>, <em>RAN1</em>, <em>COPT5</em>), aquaporins (<em>NIP2.1</em>, <em>PIP1.1</em>, <em>TIP1.1</em>, <em>TIP1.2</em>) and antioxidant defense response (<em>SOD Cu<img>Zn</em>, <em>SOD F</em>e, <em>SOD Mn</em>, <em>CAT</em>) after 1 and 7 days of exposure was analyzed. Although Cu accumulation in plant leaves was detected overtime, the Cu content in leaves exposed to nano-Cu for 7 days was 44.5% lower than in CuSO₄ at 100 mg L⁻¹. However, nano-Cu aggregates remaining on the leaf surface indicated a potential difference between measured Cu content and the real Cu pool present in the plant. Our study revealed significant changes in the pattern of gene expression overtime depending on Cu-compound type and dose. Despite the initial puzzling patterns of gene expression, after 7 days all Cu transporters showed significant down-regulation under Cu-compounds exposure to prevent Cu excess in plant cells. Conversely, aquaporin gene expression was induced after 7 days, especially by nano-Cu and CuSO₄ at 100 mg L⁻¹ due to the stimulatory effect of low Cu doses. Our study revealed that the gradual release of Cu ions from nano-Cu at a lower rate provided a milder molecular response than CuSO₄. It might indicate that nano-Cu maintained better metal balance in plants than the conventional compounds, thus may be considered as a long-term supplier of Cu.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100472"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10142781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transferability and reproducibility of exposed air-liquid interface co-culture lung models","authors":"Hedwig M. Braakhuis ,&nbsp;Eric R. Gremmer ,&nbsp;Anne Bannuscher ,&nbsp;Barbara Drasler ,&nbsp;Sandeep Keshavan ,&nbsp;Barbara Rothen-Rutishauser ,&nbsp;Barbara Birk ,&nbsp;Andreas Verlohner ,&nbsp;Robert Landsiedel ,&nbsp;Kirsty Meldrum ,&nbsp;Shareen H. Doak ,&nbsp;Martin J.D. Clift ,&nbsp;Johanna Samulin Erdem ,&nbsp;Oda A.H. Foss ,&nbsp;Shanbeh Zienolddiny-Narui ,&nbsp;Tommaso Serchi ,&nbsp;Elisa Moschini ,&nbsp;Pamina Weber ,&nbsp;Sabina Burla ,&nbsp;Pramod Kumar ,&nbsp;Rob J. Vandebriel","doi":"10.1016/j.impact.2023.100466","DOIUrl":"10.1016/j.impact.2023.100466","url":null,"abstract":"<div><h3>Background</h3><p>The establishment of reliable and robust <em>in vitro</em> models for hazard assessment, a prerequisite for moving away from animal testing, requires the evaluation of model transferability and reproducibility. Lung models that can be exposed <em>via</em> the air, by means of an air-liquid interface (ALI) are promising <em>in vitro</em> models for evaluating the safety of nanomaterials (NMs) after inhalation exposure. We performed an inter-laboratory comparison study to evaluate the transferability and reproducibility of a lung model consisting of the human bronchial cell line Calu-3 as a monoculture and, to increase the physiologic relevance of the model, also as a co-culture with macrophages (either derived from the THP-1 monocyte cell line or from human blood monocytes). The lung model was exposed to NMs using the VITROCELL® Cloud12 system at physiologically relevant dose levels.</p></div><div><h3>Results</h3><p>Overall, the results of the 7 participating laboratories are quite similar. After exposing Calu-3 alone and Calu-3 co-cultures with macrophages, no effects of lipopolysaccharide (LPS), quartz (DQ12) or titanium dioxide (TiO₂) NM-105 particles on the cell viability and barrier integrity were detected. LPS exposure induced moderate cytokine release in the Calu-3 monoculture, albeit not statistically significant in most labs. In the co-culture models, most laboratories showed that LPS can significantly induce cytokine release (IL-6, IL-8 and TNF-α). The exposure to quartz and TiO₂ particles did not induce a statistically significant increase in cytokine release in both cell models probably due to our relatively low deposited doses, which were inspired by <em>in vivo</em> dose levels. The intra- and inter-laboratory comparison study indicated acceptable interlaboratory variation for cell viability/toxicity (WST-1, LDH) and transepithelial electrical resistance, and relatively high inter-laboratory variation for cytokine production.</p></div><div><h3>Conclusion</h3><p>The transferability and reproducibility of a lung co-culture model and its exposure to aerosolized particles at the ALI were evaluated and recommendations were provided for performing inter-laboratory comparison studies. Although the results are promising, optimizations of the lung model (including more sensitive read-outs) and/or selection of higher deposited doses are needed to enhance its predictive value before it may be taken further towards a possible OECD guideline.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100466"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cocktail effects of emerging contaminants on zebrafish: Nanoplastics and the pharmaceutical diphenhydramine","authors":"Angela Barreto ,&nbsp;Joana Santos ,&nbsp;Vânia Calisto ,&nbsp;Luciana S. Rocha ,&nbsp;Mónica J.B. Amorim ,&nbsp;Vera L. Maria","doi":"10.1016/j.impact.2023.100456","DOIUrl":"10.1016/j.impact.2023.100456","url":null,"abstract":"<div><p>Nanoplastics (NPLs) became ubiquitous in the environment, from the air we breathe to the food we eat. One of the main concerns about the NPLs risks is their role as carrier of other environmental contaminants, potentially increasing their uptake, bioaccumulation and toxicity to the organisms. Therefore, the main aim of this study was to understand how the presence of polystyrene NPLs (∅ 44 nm) will influence the toxicity (synergism, additivity or antagonism) of the antihistamine diphenhydramine (DPH), towards zebrafish (<em>Danio rerio</em>) embryos, when in dual mixtures. After 96 hours (h) exposure, at the organismal level, NPLs (0.015 or 1.5 mg/L) + DPH (10 mg/L) induced embryo mortality (90%) and malformations (100%) and decreased hatching (80%) and heartbeat rates (60%). After 120 h exposure, NPLs (0.015 or 1.5 mg/L) + DPH (0.01 mg/L) decreased larvae swimming distance (30–40%). At the biochemical level, increased glutathione S-transferases (55–122%) and cholinesterase (182–343%) activities were found after 96 h exposure to NPLs (0.015 or 1.5 mg/L) + DPH (0.01 mg/L). However, catalase (CAT) activity remained similar to the control group in the mixtures, inhibiting the effects detected after the exposure to 1.5 mg/L NPLs alone (increased 230% of CAT activity). In general, the effects of dual combination – NPLs + DPH (even at concentrations as low as 10 μg/L of DPH) – were more harmful than the correspondent individual exposures, showing the synergistic interactions of the dual mixture and answering to the main question of this work. The obtained results, namely the altered toxicity patterns of NPLs + DPH compared with the individual exposures, show the importance of an environmental risk assessment considering NPLs as a co-contaminant due to the potential NPLs role as vector for other contaminants.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"30 ","pages":"Article 100456"},"PeriodicalIF":4.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9588751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zn-Al layered double hydroxides induce embryo malformations and impair locomotion behavior in Danio rerio","authors":"Diana Carneiro ,&nbsp;Évila Pinheiro Damasceno ,&nbsp;Violeta Ferreira ,&nbsp;Ives Charlie-Silva ,&nbsp;João Tedim ,&nbsp;Frederico Maia ,&nbsp;Susana Loureiro ,&nbsp;Roberto Martins ,&nbsp;Maria D. Pavlaki","doi":"10.1016/j.impact.2023.100457","DOIUrl":"10.1016/j.impact.2023.100457","url":null,"abstract":"<div><p>Layered double hydroxides (LDHs) are stimuli-responsive anionic nanoclays. The vast possibilities of using LDHs can lead to their existence in the ecosystem, raising a question of potential ecological concern. However, little is known about the effect of these nanomaterials on freshwater organisms. The present study aimed to assess the ecotoxicological effects of Zinc-Aluminium LDH-nitrate (Zn<img>Al LDH-NO₃) in zebrafish (<em>Danio rerio</em>) early life stages. The endpoints measured were mortality, malformations and hatching rate after exposure of <em>D. rerio</em> embryos and larvae to Zn<img>Al LDH-NO₃ following the OECD 236 guideline. The behavioral, biochemical (markers of oxidative stress and neurotoxicity), and molecular (at DNA level) alterations were also assessed using sub-lethal concentrations. No observable acute effects were detected up to 415.2 mg LDH/L while the 96 h-LC₅₀ was estimated as 559.9 mg/L. Tested LDH caused malformations in <em>D. rerio</em> embryos, such as pericardial edema, incomplete yolk sac absorption and tail deformities (96 h-EC₅₀ = 172.4 mg/L). During the dark periods, the locomotor behavior in zebrafish larvae was affected upon Zn<img>Al LDH-NO₃ exposure. However, no significant biochemical and molecular changes were recorded. The present findings suggest that Zn<img>Al LDH-NO₃ can be regarded as a non-toxic nanomaterial towards <em>D. rerio</em> (E/LC₅₀ &gt; &gt; 100 mg/L) although impairment of the locomotion behavior on zebrafish embryos can be expected at concentrations below 100 mg/L.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"30 ","pages":"Article 100457"},"PeriodicalIF":4.9,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9580039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}