超顺磁性氧化铁纳米粒子对睾丸细胞的毒性:体外研究。

IF 4.7 3区 环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES
Graziela de P.F. Dantas , Fausto S. Ferraz , John L.P. Coimbra , Roberto M. Paniago , Maria S.S. Dantas , Samyra M.S.N. Lacerda , Marcela S. Procópio , Matheus F. Gonçalves , Marcelo H. Furtado , Bárbara P. Mendes , Jorge L. López , Alisson C. Krohling , Estefânia M.N. Martins , Lídia M. Andrade , Luiz O. Ladeira , Ângela L. Andrade , Guilherme M.J. Costa
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引用次数: 0

摘要

超顺磁性氧化铁纳米粒子(SPIONs)因其潜在的应用而在生物医学研究中备受关注。然而,人们对其对睾丸细胞的影响和毒性知之甚少。为了解决这个问题,我们利用小鼠原代睾丸细胞、睾丸碎片和精子进行了一项体外研究,以探讨柠檬酸钠包被的 SPIONs(Cit_SPIONs)的细胞毒性效应。在此,我们合成了Cit_SPIONs并对其进行了物理化学表征,观察到柠檬酸钠在实验过程中减小了纳米粒子的尺寸并提高了其在溶液中的稳定性。在使用浓度(3%)下,柠檬酸钠(通过热重计测量)与睾丸细胞具有生物相容性。尽管Cit_SPIONs具有这些良好的理化特性,但体外实验表明它具有细胞毒性,尤其是对睾丸体细胞和精子细胞。透射电子显微镜分析证实,在器官型培养系统中,Leydig 细胞优先内化了 Cit_SPIONs,这导致了它们细胞质大小的改变。此外,我们还发现,接触 Cit_SPIONs 对精子细胞的各种参数都有不利影响,包括活力、存活率、DNA 完整性、线粒体活性、脂质过氧化(LPO)和 ROS 生成。我们的研究结果表明,睾丸体细胞和精子细胞对 Cit_SPIONs 和诱导的氧化应激非常敏感和脆弱。这项研究强调了 SPIONs 的潜在毒性,表明其对男性生殖系统具有重大威胁。我们的研究结果凸显了详细开发氧化铁纳米粒子以提高生殖纳米安全性的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study

The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study

Superparamagnetic iron oxide nanoparticles (SPIONs) have gained significant attention in biomedical research due to their potential applications. However, little is known about their impact and toxicity on testicular cells. To address this issue, we conducted an in vitro study using primary mouse testicular cells, testis fragments, and sperm to investigate the cytotoxic effects of sodium citrate-coated SPIONs (Cit_SPIONs). Herein, we synthesized and physiochemically characterized the Cit_SPIONs and observed that the sodium citrate diminished the size and improved the stability of nanoparticles in solution during the experimental time. The sodium citrate (measured by thermogravimetry) was biocompatible with testicular cells at the used concentration (3%). Despite these favorable physicochemical properties, the in vitro experiments demonstrated the cytotoxicity of Cit_SPIONs, particularly towards testicular somatic cells and sperm cells. Transmission electron microscopy analysis confirmed that Leydig cells preferentially internalized Cit_SPIONs in the organotypic culture system, which resulted in alterations in their cytoplasmic size. Additionally, we found that Cit_SPIONs exposure had detrimental effects on various parameters of sperm cells, including motility, viability, DNA integrity, mitochondrial activity, lipid peroxidation (LPO), and ROS production. Our findings suggest that testicular somatic cells and sperm cells are highly sensitive and vulnerable to Cit_SPIONs and induced oxidative stress. This study emphasizes the potential toxicity of SPIONs, indicating significant threats to the male reproductive system. Our findings highlight the need for detailed development of iron oxide nanoparticles to enhance reproductive nanosafety.

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来源期刊
NanoImpact
NanoImpact Social Sciences-Safety Research
CiteScore
11.00
自引率
6.10%
发文量
69
审稿时长
23 days
期刊介绍: NanoImpact is a multidisciplinary journal that focuses on nanosafety research and areas related to the impacts of manufactured nanomaterials on human and environmental systems and the behavior of nanomaterials in these systems.
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