NanoImpact最新文献

{"title":"Comparative life cycle assessment of the fabrication of visible-light-driven photocatalytic TiO2 – Carbon dots nanocomposites for wastewater treatment","authors":"Ricardo M.S. Sendão ,&nbsp;Joaquim C.G. Esteves da Silva ,&nbsp;Luís Pinto da Silva","doi":"10.1016/j.impact.2025.100556","DOIUrl":"10.1016/j.impact.2025.100556","url":null,"abstract":"<div><div>TiO₂ nanoparticles are promising photocatalysts due to their oxidizing strength and inertness. However, their inability to efficiently absorb visible light limits industrial applications that could use sunlight. The addition of carbon dots to TiO₂ has been recently shown to have the potential to address this issue by enhancing the visible-light-driven photocatalytic efficiency of the resulting nanocomposites. However, concrete data on their sustainable development and fabrication is lacking. Herein, we performed a life cycle assessment (LCA) study to understand the environmental impacts of different TiO₂‑carbon dots nanocomposites with enhanced visible-light-driven photocatalytic activity. It was found that the nanocomposites with the highest photocatalytic activity were the ones whose synthesis was associated with lower environmental impacts. Furthermore, the carbon dots generally have little to moderate contributions to most impact categories, except for marine eutrophication. Finally, the most critical parameter was found to be the source of TiO₂ for the nanocomposites.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100556"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143776664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Navigating nanotoxicity: Unraveling nanomaterial-induced effects via multi-omics integration","authors":"Zhihui Wang,&nbsp;Ting Zhang,&nbsp;Meng Tang","doi":"10.1016/j.impact.2025.100565","DOIUrl":"10.1016/j.impact.2025.100565","url":null,"abstract":"<div><div>The growing use of nanomaterials in industry and medicine raises significant concerns about their safety, particularly regarding their interactions with biological systems. Traditional toxicological methods, with limited throughput and mechanistic understanding, are increasingly being complemented by omics technologies. Genomics, transcriptomics, proteomics, and metabolomics provide comprehensive insights into the molecular mechanisms of nanomaterial toxicity and enable the identification of potential biomarkers. In addition, single-cell and spatial omics approaches are emerging as powerful tools to assess toxicity at the cellular and tissue levels, revealing heterogeneous responses and spatial distribution of nanomaterials. Despite their advantages, omics technologies face challenges in nanotoxicology, including large, complex data sets, integration difficulties, and a lack of standardized protocols. To address these challenges, we propose the development of new bioinformatics tools, multi-omics integration platforms, and standardized analysis processes to enhance research efficiency and accuracy. These efforts can provide a practical roadmap for integrating the application of omics technologies, including single-cell and spatial approaches, in the study of nanomaterial toxicity studies.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100565"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144089625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico exploration of graphene nanoflakes: From DFT simulations to machine learning-driven toxicity predictions","authors":"Nuria Aguilar ,&nbsp;Patricia de la Fuente ,&nbsp;Natalia Fernández-Pampín ,&nbsp;Sonia Martel ,&nbsp;Laura Gómez-Cuadrado ,&nbsp;Pedro Angel Marcos ,&nbsp;Alfredo Bol ,&nbsp;Carlos Rumbo ,&nbsp;Santiago Aparicio","doi":"10.1016/j.impact.2025.100563","DOIUrl":"10.1016/j.impact.2025.100563","url":null,"abstract":"<div><div>The present theoretical work provides a ground-breaking and comprehensive study of graphene nanoflakes integrating Density Functional Theory (DFT) simulations, toxicity predictions and a machine learning approach. The properties of graphene nanoflakes as a function of size, shape, and symmetry are systematically analysed using DFT calculations. The interaction of these nanoflakes with human proteins and cell membranes, considered as Molecular Initiating Events for diverse Adverse Outcome Pathways, is explored to infer potential toxicity effects. Leveraging the generated data, machine learning models were developed to predict flake properties and biological interactions. A single score representing the biological interaction or impact of graphene nanoflakes on both proteins and plasma membranes is assigned to each evaluated nanoflake to infer its potential toxicity. Our multiscale approach bring valuable insights into the structure-property-toxicity relationships of graphene nanoflakes, paving the way for their safe and efficient design and application.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100563"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143895547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vertical distribution of microplastics in soil affects plant response to microplastics.","authors":"Merve Tunali ,&nbsp;Matthias C. Rillig","doi":"10.1016/j.impact.2025.100557","DOIUrl":"10.1016/j.impact.2025.100557","url":null,"abstract":"<div><div>The impacts of microplastics on plants have been extensively researched, yielding a variety of responses: promoting growth, limiting growth, or causing no change in plants. Experimental studies, following basic principles of ecotoxicology, typically use a homogeneous distribution of microplastics in soils, where soil and microplastic are well-mixed. However, in the environment, plastic is not homogeneously distributed. Therefore, we tested whether the distribution of microplastics in soils affects the impact observed on plants. For this purpose, we tested the effect of homogeneously distributed microplastics and heterogeneously distributed microplastics (at different levels) on the growth of spring onions. In addition, the presence of drought was also included in our greenhouse experiment. The results show that the distribution of microplastics (whether it is homogeneous or heterogeneous) affects the growth of spring onions differently, especially the shoot and root mass. First, differences of 21–22 % in shoot mass and 29–38 % in root mass were observed between heterogeneously distributed treatments and the homogeneous treatment. Second, under drought conditions, the effects -particularly on shoot mass and the C:N (carbon:nitrogen) ratio- may differ compared to non-drought. Differences of 30–37 % in shoot mass, and up to 16 % in the carbon/nitrogen ratio were observed between different heterogeneously distributed treatments and the homogeneous treatment in the drought case. In addition, shoot mass and the C:N ratio varied depending on drought conditions. Our results strongly suggest that future experiments on microplastic effects in soil should consider at least vertically heterogeneity of the pollutant to arrive at more realistic effect estimates.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100557"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A model for screening the fate and behaviour of the engineered nanoparticles in aquatic systems using semi-quantitative analysis and rule-based system","authors":"Ntsikelelo Yalezo,&nbsp;Michael O. Daramola","doi":"10.1016/j.impact.2025.100564","DOIUrl":"10.1016/j.impact.2025.100564","url":null,"abstract":"<div><div>Concerns over the possible adverse effects of engineered nanoparticles (ENPs) on aquatic organisms have grown due to their continuous emission into aquatic systems. Consequently, to safeguard these aquatic life forms and support the sustainable use of ENPs, the characterisation of their exposure is necessary. Currently, despite the great amount of work reported to elucidate the exposure and risks of ENPs, cost-effective and easy-to-use exposure characterisation models are lacking and scarce. This study describes the use of semi-quantitative analysis (SQA) integrated with a rule-based system to evaluate ENP exposure in aquatic systems. The performance of the model was illustrated using case studies of nZnO, nTiO₂, and nAg and theoretical examples that simulate natural systems. The results demonstrate that our proposed model can be highly valuable as an alternative approach for the preliminary screening of the exposure and possible environmental impact of ENPs in aquatic systems. The SQA application is relatively cost-effective and easy to use, since no software or mathematical computations are required. In addition, non-experts can easily understand the hierarchical nature, Boolean logic, and visual representations of simple rules using decision trees; which is highly valuable given that testing each variation of ENPs is tedious and associated with high cost.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100564"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of inflammatory and mesothelioma-related responses in mice following the intraperitoneal administration of cellulose nanofibers","authors":"Masanori Horie ,&nbsp;Sakiko Sugino ,&nbsp;Katsuhide Fujita ,&nbsp;Shigehisa Endoh ,&nbsp;Junko Maru ,&nbsp;Tomohiko Matsuzawa ,&nbsp;Isamu Ogura","doi":"10.1016/j.impact.2025.100561","DOIUrl":"10.1016/j.impact.2025.100561","url":null,"abstract":"<div><div>Mesothelioma can develop from long-term exposure to fine fibrous materials including asbestos and carbon nanotubes, and chronic inflammation is critical for its development. Inflammatory responses and key related markers associated with the development of mesothelioma were evaluated over time in relation to cellulose nanofibers (CNFs), which are being developed as new plant-derived materials, for the purpose of risk management Three types of CNFs, mechanically fibrillated CNFs, TEMPO-oxidized CNFs, and phosphorylated CNFs, were administered intraperitoneally to mice at doses of 0.1 and 1.0 mg per animal, and progress was observed for up to 2 years. In the group receiving mechanically fibrillated CNFs, white substances were observed adhering to the liver surface throughout the observation period. This white substance was presumed to be cellulose. Some CNFs have been shown to persist in the body. During this period, inflammation markers and mesothelioma-related markers were evaluated at 1, 2, and 6 months, and 1, and 2 years after administration. No significant symptoms were observed in animals administered one of the three types of CNFs intraperitoneally during the observation period. Inflammatory markers in the peritoneal lavage fluid remained below the detection limit throughout the entire observation period. Additionally, no significant increase in blood levels of mesothelioma or the major related markers mesothelin, osteopontin, or HMGB1 was observed. In this study, although some CNFs remained in the body, no inflammatory response was observed <em>in vivo</em> under the concentration conditions and observation periods used. Furthermore, no evidence of long-term effects, such as cancer, was found.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100561"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A meta-analysis of nanomaterial and nanoplastic fate in small column experiments and implications for fate in soils","authors":"Geert Cornelis","doi":"10.1016/j.impact.2025.100558","DOIUrl":"10.1016/j.impact.2025.100558","url":null,"abstract":"<div><div>A long list of possible processes may simultaneously control retention of engineered nanomaterials (NMs) and nanoplastics (NPs) in soils, but there is little insight in which of these processes dominates and under which circumstances. Though not easily transferable to field situations, repacked saturated column tests compose the richest available dataset to explore for overall trends in the behaviour of NMs and NPs in soils. Global attachment efficiencies (<em>α</em>_global) were calculated uniformly from metadata of 624 column experiments and correlated against metadata using partial least squares and linear regression analysis. <em>α</em>_global values appeared to some extent operationally defined as they correlate with the experimental column flow rate and in some cases with the particle concentration used in the feedstock. Particle aggregation occurred more as the feedstock concentration increased, but this only had a limited effect on subsequent column retention. In homogeneous sandy media, attachment of particles obeyed well-known trends indicative of non-favourable electrostatic interactions, whereas interactions in non-sandy media were dominated by favourable attractions to positively charged sites on clay edges and/or oxides as well as hydrophobic interactions with soil organic matter. The results may help to prioritize further research such as the currently unclear role of hydrophobic interactions in the fate of particles in porous media and identify the most important transport processes in more complex field situations.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100558"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From literature to predictive modeling: Insights and machine learning applications from in vitro comet assays related to the genotoxicity of titanium dioxide nanomaterials","authors":"Irini Furxhi ,&nbsp;Mahsa Mirzaei ,&nbsp;Anna Costa ,&nbsp;Rossella Bengalli","doi":"10.1016/j.impact.2025.100562","DOIUrl":"10.1016/j.impact.2025.100562","url":null,"abstract":"<div><div>The genotoxicity of titanium dioxide nanomaterials (TiO₂ NMs) remains a debated topic in the scientific community. In this study, we applied the read-across concept based on machine learning (ML) algorithms to predict the genotoxic potential of TiO₂ NMs. Key objectives included: (i) compiling a systematic dataset capturing DNA damage percentage from in vitro comet assays, (ii) creating a homogenized dataset integrating physicochemical properties, exposure conditions, and experimental details, (iii) training ML models for prediction, (iv) evaluating model performance, and (v) identifying the features that contribute the most to predictive accuracy. The dataset was divided into three parts: the Entire dataset (all features), the Physicochemical dataset, and the Experimental design dataset. Extra Trees Regressor and XGB Regressor demonstrated high predictive accuracy, achieving R² values of 0.906 and 0.788 for the P-chem and Experimental dataset, respectively. Exposure concentration, cold lysis conditions, and electrophoresis parameters emerged as key predictors of DNA damage, alongside contributions from NM properties. These findings highlight the intricate interplay between NM properties and experimental conditions in genotoxicity assessments. By providing a FAIR dataset, this study facilitates future research, allowing for the integration of additional variables and quality criteria to enhance the modeling approach. This work reinforces the value of nano-informatics in nanosafety and serves as a footing for advancing data-driven hazard assessment methodologies, positioning ML-enabled read-across strategies as a valuable tool for regulatory nanosafety framework.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100562"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between polystyrene nanoparticles and human intestinal epithelial Caco-2 cells","authors":"Yuan-Yuan Liu,&nbsp;Jie Liu,&nbsp;Yuan Guo,&nbsp;Qiangqiang Zhang,&nbsp;Aoneng Cao,&nbsp;Haifang Wang","doi":"10.1016/j.impact.2025.100559","DOIUrl":"10.1016/j.impact.2025.100559","url":null,"abstract":"<div><div>Nanoplastics enter the human body mainly by ingestion through the gastrointestinal tract and thus the uptake and release of nanoplastics in intestinal cells have been studied. However, the fate of nanoplastics in intestinal cells remains poorly understood, particularly how they are exocytosed. Herein, we investigated the uptake, distribution, and exocytosis of nanoplastics in Caco-2 cells using 70 nm red fluorescent polystyrene (R70PS) as a nanoplastic model. The results show that R70PS readily enters Caco-2 cells and the content per cell peaks at around 24 h, but the total intracellular content in all cells increases continuously over 72 h. In addition, the uptake mechanisms change over incubation time, i.e. R70PS entered Caco-2 cells via both the energy-independent pathway and the energy-dependent caveolae-mediated endocytosis and macropinocytosis at 4 h incubation, but almost all R70PS entered cells in an energy-dependent manner via caveolae-mediated endocytosis, macropinocytosis, and clathrin-mediated endocytosis at 12 h incubation. Most of the intracellular R70PS accumulated in lysosomes, but R70PS also entered the mitochondria and its level increased over time. Approximately 45 % of the intracellular R70PS could be cleared from the cells within 12 h, mainly via the lysosomal pathway. Exocytosis was also associated with autophagy and was facilitated by the increase in the number of mitochondria and lysosomes, but inhibited by serum in the medium. Our findings deepen the understanding of the interaction between nanoplastics and intestinal cells, which is helpful for the risk assessment of nanoplastics.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100559"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dispersion protocols have minimal impact on the biomolecular corona of advanced nanomaterials in cell culture assays","authors":"Alberto Martinez-Serra ,&nbsp;Asia Saorin ,&nbsp;Ana Serrano-Lotina ,&nbsp;Ahmed Subrati ,&nbsp;Mahmoud G. Soliman ,&nbsp;Danail Hristozov ,&nbsp;Miguel A. Bañares ,&nbsp;Philip Demokritou ,&nbsp;Marco P. Monopoli","doi":"10.1016/j.impact.2025.100560","DOIUrl":"10.1016/j.impact.2025.100560","url":null,"abstract":"<div><div>Industrial sectors have largely invested in the use of advanced nanomaterials (NMs), which are currently being implemented in a wide range of applications. However, the potential exposure to living beings and the environment still remains a concern. While some of these materials were not designed to be dispersible in aqueous media, the development of dispersion protocols to ensure compatibility with the <em>in vitro</em> and <em>in vivo</em> assays has become crucial for the correct assessment of the studies. NMs' identity in biological media is significantly influenced by the formation of a biomolecular corona on its surface. However, this corona might be affected by the dispersion method, altering their physicochemical characteristics and complicating the understanding of their interactions with biological systems. Therefore, understanding the efficiency of dispersion protocols and their influence on the biological identity of NMs is fundamental. However, systematic studies on the effects of dispersion protocols are still lacking, making this a crucial yet overlooked aspect in the field. This study aims to compare two standard dispersion protocols, commonly known as Harvard and Nanogenotox, and evaluate their impact on the biomolecular corona formation across a selection of advanced industrial NMs. To this aim, different techniques were used to assess particle size, colloidal stability and ion release, as well as protein and sialic acid content and abundance in the corona. Results show that the dispersion protocol modestly alters nanoparticle size and agglomeration state, and proteomics analysis revealed that each nanoparticle type forms a distinct corona, influenced by the distinct surface modifications. The presence of bovine serum albumin (BSA) in the Nanogenotox protocol minimally affected the overall trends in protein composition between the two protocols. These findings emphasize the significance of the dispersion protocol in nanotoxicology assays and demonstrate that variations between these methods do not play a decisive role in shaping the bio-identity and potential biological effects of advanced and multicomponent NMs.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100560"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}