NanoImpact最新文献

{"title":"Evaluation of inflammatory and mesothelioma-related responses in mice following the intraperitoneal administration of cellulose nanofibers","authors":"Masanori Horie ,&nbsp;Sakiko Sugino ,&nbsp;Katsuhide Fujita ,&nbsp;Shigehisa Endoh ,&nbsp;Junko Maru ,&nbsp;Tomohiko Matsuzawa ,&nbsp;Isamu Ogura","doi":"10.1016/j.impact.2025.100561","DOIUrl":"10.1016/j.impact.2025.100561","url":null,"abstract":"<div><div>Mesothelioma can develop from long-term exposure to fine fibrous materials including asbestos and carbon nanotubes, and chronic inflammation is critical for its development. Inflammatory responses and key related markers associated with the development of mesothelioma were evaluated over time in relation to cellulose nanofibers (CNFs), which are being developed as new plant-derived materials, for the purpose of risk management Three types of CNFs, mechanically fibrillated CNFs, TEMPO-oxidized CNFs, and phosphorylated CNFs, were administered intraperitoneally to mice at doses of 0.1 and 1.0 mg per animal, and progress was observed for up to 2 years. In the group receiving mechanically fibrillated CNFs, white substances were observed adhering to the liver surface throughout the observation period. This white substance was presumed to be cellulose. Some CNFs have been shown to persist in the body. During this period, inflammation markers and mesothelioma-related markers were evaluated at 1, 2, and 6 months, and 1, and 2 years after administration. No significant symptoms were observed in animals administered one of the three types of CNFs intraperitoneally during the observation period. Inflammatory markers in the peritoneal lavage fluid remained below the detection limit throughout the entire observation period. Additionally, no significant increase in blood levels of mesothelioma or the major related markers mesothelin, osteopontin, or HMGB1 was observed. In this study, although some CNFs remained in the body, no inflammatory response was observed <em>in vivo</em> under the concentration conditions and observation periods used. Furthermore, no evidence of long-term effects, such as cancer, was found.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100561"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From literature to predictive modeling: Insights and machine learning applications from in vitro comet assays related to the genotoxicity of titanium dioxide nanomaterials","authors":"Irini Furxhi ,&nbsp;Mahsa Mirzaei ,&nbsp;Anna Costa ,&nbsp;Rossella Bengalli","doi":"10.1016/j.impact.2025.100562","DOIUrl":"10.1016/j.impact.2025.100562","url":null,"abstract":"<div><div>The genotoxicity of titanium dioxide nanomaterials (TiO₂ NMs) remains a debated topic in the scientific community. In this study, we applied the read-across concept based on machine learning (ML) algorithms to predict the genotoxic potential of TiO₂ NMs. Key objectives included: (i) compiling a systematic dataset capturing DNA damage percentage from in vitro comet assays, (ii) creating a homogenized dataset integrating physicochemical properties, exposure conditions, and experimental details, (iii) training ML models for prediction, (iv) evaluating model performance, and (v) identifying the features that contribute the most to predictive accuracy. The dataset was divided into three parts: the Entire dataset (all features), the Physicochemical dataset, and the Experimental design dataset. Extra Trees Regressor and XGB Regressor demonstrated high predictive accuracy, achieving R² values of 0.906 and 0.788 for the P-chem and Experimental dataset, respectively. Exposure concentration, cold lysis conditions, and electrophoresis parameters emerged as key predictors of DNA damage, alongside contributions from NM properties. These findings highlight the intricate interplay between NM properties and experimental conditions in genotoxicity assessments. By providing a FAIR dataset, this study facilitates future research, allowing for the integration of additional variables and quality criteria to enhance the modeling approach. This work reinforces the value of nano-informatics in nanosafety and serves as a footing for advancing data-driven hazard assessment methodologies, positioning ML-enabled read-across strategies as a valuable tool for regulatory nanosafety framework.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100562"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between polystyrene nanoparticles and human intestinal epithelial Caco-2 cells","authors":"Yuan-Yuan Liu,&nbsp;Jie Liu,&nbsp;Yuan Guo,&nbsp;Qiangqiang Zhang,&nbsp;Aoneng Cao,&nbsp;Haifang Wang","doi":"10.1016/j.impact.2025.100559","DOIUrl":"10.1016/j.impact.2025.100559","url":null,"abstract":"<div><div>Nanoplastics enter the human body mainly by ingestion through the gastrointestinal tract and thus the uptake and release of nanoplastics in intestinal cells have been studied. However, the fate of nanoplastics in intestinal cells remains poorly understood, particularly how they are exocytosed. Herein, we investigated the uptake, distribution, and exocytosis of nanoplastics in Caco-2 cells using 70 nm red fluorescent polystyrene (R70PS) as a nanoplastic model. The results show that R70PS readily enters Caco-2 cells and the content per cell peaks at around 24 h, but the total intracellular content in all cells increases continuously over 72 h. In addition, the uptake mechanisms change over incubation time, i.e. R70PS entered Caco-2 cells via both the energy-independent pathway and the energy-dependent caveolae-mediated endocytosis and macropinocytosis at 4 h incubation, but almost all R70PS entered cells in an energy-dependent manner via caveolae-mediated endocytosis, macropinocytosis, and clathrin-mediated endocytosis at 12 h incubation. Most of the intracellular R70PS accumulated in lysosomes, but R70PS also entered the mitochondria and its level increased over time. Approximately 45 % of the intracellular R70PS could be cleared from the cells within 12 h, mainly via the lysosomal pathway. Exocytosis was also associated with autophagy and was facilitated by the increase in the number of mitochondria and lysosomes, but inhibited by serum in the medium. Our findings deepen the understanding of the interaction between nanoplastics and intestinal cells, which is helpful for the risk assessment of nanoplastics.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100559"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dispersion protocols have minimal impact on the biomolecular corona of advanced nanomaterials in cell culture assays","authors":"Alberto Martinez-Serra ,&nbsp;Asia Saorin ,&nbsp;Ana Serrano-Lotina ,&nbsp;Ahmed Subrati ,&nbsp;Mahmoud G. Soliman ,&nbsp;Danail Hristozov ,&nbsp;Miguel A. Bañares ,&nbsp;Philip Demokritou ,&nbsp;Marco P. Monopoli","doi":"10.1016/j.impact.2025.100560","DOIUrl":"10.1016/j.impact.2025.100560","url":null,"abstract":"<div><div>Industrial sectors have largely invested in the use of advanced nanomaterials (NMs), which are currently being implemented in a wide range of applications. However, the potential exposure to living beings and the environment still remains a concern. While some of these materials were not designed to be dispersible in aqueous media, the development of dispersion protocols to ensure compatibility with the <em>in vitro</em> and <em>in vivo</em> assays has become crucial for the correct assessment of the studies. NMs' identity in biological media is significantly influenced by the formation of a biomolecular corona on its surface. However, this corona might be affected by the dispersion method, altering their physicochemical characteristics and complicating the understanding of their interactions with biological systems. Therefore, understanding the efficiency of dispersion protocols and their influence on the biological identity of NMs is fundamental. However, systematic studies on the effects of dispersion protocols are still lacking, making this a crucial yet overlooked aspect in the field. This study aims to compare two standard dispersion protocols, commonly known as Harvard and Nanogenotox, and evaluate their impact on the biomolecular corona formation across a selection of advanced industrial NMs. To this aim, different techniques were used to assess particle size, colloidal stability and ion release, as well as protein and sialic acid content and abundance in the corona. Results show that the dispersion protocol modestly alters nanoparticle size and agglomeration state, and proteomics analysis revealed that each nanoparticle type forms a distinct corona, influenced by the distinct surface modifications. The presence of bovine serum albumin (BSA) in the Nanogenotox protocol minimally affected the overall trends in protein composition between the two protocols. These findings emphasize the significance of the dispersion protocol in nanotoxicology assays and demonstrate that variations between these methods do not play a decisive role in shaping the bio-identity and potential biological effects of advanced and multicomponent NMs.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100560"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Death-driven exposure: Necrotrophic transfer of polylactic acid and polystyrene microplastics alters biochemical architecture in the saprophagous insect Chrysomya megacephala","authors":"Ariane Guimarães ,&nbsp;Raíssa Ferreira de Oliveira ,&nbsp;Abraão Tiago Batista Guimarães ,&nbsp;Rafaela Ribeiro de Brito ,&nbsp;Aline Sueli de Lima Rodrigues ,&nbsp;Letícia Paiva de Matos ,&nbsp;Thiarlen Marinho da Luz ,&nbsp;Guilherme Malafaia","doi":"10.1016/j.impact.2025.100570","DOIUrl":"10.1016/j.impact.2025.100570","url":null,"abstract":"<div><div>Microplastic exposure (MPs) represents an emerging threat to terrestrial ecotoxicology, particularly within detritivorous ecosystems. In this study, we investigated transstadial bioaccumulation and the biochemical effects of necrotrophic exposure to polystyrene (PS-MPs) and polylactic acid microplastics (PLA-MPs) in <em>Chrysomya megacephala</em>. Mice contaminated with varying concentrations of the polymers were used as larval decomposition substrates. To confirm the prior internalization of MPs, third-instar larvae (L3) were analyzed, revealing significant particle accumulation. We assessed MP retention and biochemical alterations in key physiological systems following adult emergence. The presence of MPs in newly emerged adults, at significantly lower concentrations than those observed in larvae, confirmed transstadial bioaccumulation and indicated partial retention during metamorphosis. Bioaccumulation was more pronounced at higher exposure concentrations. PS-MPs induced functional disorganization, characterized by increased reactive oxygen species (ROS), lipid peroxidation, proteolytic activity, and neuroendocrine dysfunctions. In contrast, PLA-MPs elicited more coordinated biochemical responses, with emphasis on antioxidant activation. Correlation network and functional trade-off analyses revealed distinct patterns of physiological reorganization between treatments. At the same time, trend tests and causal inference confirmed concentration-dependent effects and a direct association between exposure and biochemical dysfunction. The data suggest that necrotrophic exposure constitutes a functional pathway for MP redistribution in terrestrial environments, exerting systemic effects even in the absence of direct adult contact.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100570"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics: Hidden drivers of antimicrobial resistance in aquatic systems","authors":"Prasun Goswami ,&nbsp;Kazuki Kanda ,&nbsp;Yukino Tamamura-Andoh ,&nbsp;Mafumi Watanabe ,&nbsp;Keerthi S. Guruge","doi":"10.1016/j.impact.2025.100566","DOIUrl":"10.1016/j.impact.2025.100566","url":null,"abstract":"<div><div>Microplastics (MPs) in aquatic ecosystems readily promote biofilm formation, creating the plastisphere, a dynamic interface that interacts with environmental pollutants and acts as a reservoir for microorganisms. Recent studies emphasize the plastisphere's contribution to the spread of pathogens, antibiotic-resistant genes (ARGs), and antimicrobial resistance (AMR) within aquatic organisms and across diverse environments, a phenomenon collectively called the ‘Plastiome’. Although the prevalence and effects of the plastisphere have been studied extensively, a systematic synthesis of updated insights into the behavior of the plastiome is urgently needed. This review explores the development and behavior of plastics, focusing on its interactions with ARGs and pathogens within aquatic ecosystems. Microplastics selectively enrich ARGs and pathogenic microorganisms, fostering unique microbial communities distinct from those in surrounding waters. The plastiome facilitates horizontal ARG propagation, increasing the quantity of antibiotic-resistant pathogens and presenting substantial risks to the hydrosphere and public health. Additionally, key research opportunities are identified and strategies are recommended to advance our understanding of plastiome-driven antibiotic resistance in aquatic environments.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100566"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144084449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of reference environmentally relevant micro-nano-plastics for risk assessment studies","authors":"Milton Das ,&nbsp;Leonardo Calderon ,&nbsp;Dilpreet Singh ,&nbsp;Satwik Majumder ,&nbsp;Lila Bazina ,&nbsp;Nachiket Vaze ,&nbsp;Una Trivanovic ,&nbsp;Glen DeLoid ,&nbsp;Nubia Zuverza-Mena ,&nbsp;Mandeep Kaur ,&nbsp;Jakub Konkol ,&nbsp;Nassifatou Koko Tittikpina ,&nbsp;George Tsilomelekis ,&nbsp;Omowunmi Sadik ,&nbsp;Jason C. White ,&nbsp;Philip Demokritou","doi":"10.1016/j.impact.2025.100567","DOIUrl":"10.1016/j.impact.2025.100567","url":null,"abstract":"<div><div>Micro- and nano-plastics (MNPs) have become ubiquitous environmental pollutants. Extensive toxicological studies of MNPs have been conducted in recent years. However, because of the difficulties involved in extraction and collection of MNPs from environmental media, most of these studies have employed simplistic, pristine, spherical, micro- or nano-sized commercial MNPs, whose properties, including morphology, surface chemistry, and size, do not adequately approximate those of environmentally relevant MNPs. Here, we describe the development and use of methods for the synthesis of well characterized, environmentally relevant MNPs across the life cycle of a plastic material, in a property-controlled manner. Multiple degradation scenarios, including mechanical fragmentation (cryogenic milling), UV weathering, and thermal disintegration (incineration) were applied to virgin plastic materials (polyvinyl chloride, PVC; polyethylene terephthalate, PET; High density polyethylene, HDPE; Acrylonitrile butadiene styrene, ABS; polycarbonate, PC; and polypropylene, PP) to simulate the life cycle pathways that likely occur in the environment over time. The MNPs generated from these degradation processes were size fractionated using both “dry” and “wet” separation methods. Detailed physicochemical characterization of the size fractionated reference MNPs was performed to determine size, morphology, chemical and elemental compositions, and hydrophobicity. Microbiological sterility and endotoxin content of reference MNPs were also assessed. Protocols for storage of reference MNPs in controlled oxygen and moisture conditions for future use in toxicological studies are also described. The methodology developed in this study can be used to synthesize environmentally relevant reference MNPs across the life cycle of plastic materials for use in risk assessment studies.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100567"},"PeriodicalIF":4.7,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144240428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scaling up the graphene production from R&D to the pilot plant stage: Implications for workers' exposure to airborne nano-objects","authors":"Claudio Natale ,&nbsp;Francesca Tombolini ,&nbsp;Riccardo Ferrante ,&nbsp;Francesca Sebastiani ,&nbsp;Andrea Gordiani ,&nbsp;Maurizio Manigrasso ,&nbsp;Antonio Esau Del Rio Castillo ,&nbsp;Francesco Bonaccorso ,&nbsp;Stefania Sabella ,&nbsp;Fabio Boccuni","doi":"10.1016/j.impact.2025.100555","DOIUrl":"10.1016/j.impact.2025.100555","url":null,"abstract":"<div><div>Given the exceptional thermal, electrical, and mechanical properties of graphene, the interest is now shifting from scientific and technological application to industrial deployment, testified by the significant increase in demand for graphene-based products. Consequently, it is paramount that occupational safety and health (OSH) research now places utmost importance on ensuring the well-being of workers at every stage of graphene production. The present study evaluates workers' exposure potential during the production cycle of few-layer graphene (FLG) by liquid-phase exfoliation, incorporating the Prevention-through-Design approach in the transition from the laboratory scale to the pilot plant production. A measurement campaign was conducted according to the multi-metric approach proposed by the Organization for Economic Cooperation and Development and European Committee for Standardization guidelines. Multi-metric real-time instruments were used to determine particle number concentration (PNC), particle size distribution and lung deposited surface area (LDSA) along with time-integrated instrumentation to collect airborne ultrafine dust for off-line gravimetric analysis and chemical and morphological characterization. The obtained data indicate that the FLG powders storage, including the cleaning of equipment and surfaces, is the most critical step for exposed workers, with higher levels of PNC and LDSA compared to the other production phases. Recommendations for OSH risk mitigation strategies in the scaling up of the FLG production process have been proposed according to OSH principles for nano and advanced materials development. In particular, production and storage of FLG in liquid suspension or bound to a solid matrix should be preferred rather than in powder form. When not possible, a closed system with local exhaust ventilation is recommended. Finally, if the particles transport towards other areas of the plant is not properly mitigated, the sole use of personal protective equipment during the powder handling phases will be not sufficient for protecting workers from the potential exposure.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"38 ","pages":"Article 100555"},"PeriodicalIF":4.7,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143673335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations","authors":"Michael J. Burgum ,&nbsp;Víctor Alcolea-Rodríguez ,&nbsp;Hanna Saarelainen ,&nbsp;Raquel Portela ,&nbsp;Julián J. Reinosa ,&nbsp;José F. Fernández ,&nbsp;Verónica I. Dumit ,&nbsp;Julia Catalán ,&nbsp;Felice C. Simeone ,&nbsp;Lara Faccani ,&nbsp;Martin J.D. Clift ,&nbsp;Stephen J. Evans ,&nbsp;Miguel A. Bañares ,&nbsp;Shareen H. Doak","doi":"10.1016/j.impact.2024.100539","DOIUrl":"10.1016/j.impact.2024.100539","url":null,"abstract":"<div><div>Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the <em>in vitro</em> mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o⁻ human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24 h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o⁻ with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox <em>vs.</em> high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized <em>in chemico</em> as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"37 ","pages":"Article 100539"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of polyglycolic acid and analogues on glycolipid metabolism and circadian behavior in zebrafish","authors":"Liang Wen ,&nbsp;Shuhui Zhang ,&nbsp;Jialu Luan ,&nbsp;Tian Yin ,&nbsp;Xizeng Feng","doi":"10.1016/j.impact.2025.100546","DOIUrl":"10.1016/j.impact.2025.100546","url":null,"abstract":"<div><div>For the past few years, new biodegradable polymers, such as polyglycolic acid (PGA) and polylactic acid (PLA), have been promising materials to solve the remarkable environmental issue, of microplastics (MPs) pollution. In this research, the impacts of five MPs, including PGA, PLA, polybutylene succinate (PBS), polyhydroxyalkanoate (PHA), and polybutylene adipate terephthalate (PBAT), were analyzed on zebrafish with different concentrations. We found that PGA and PLA at 1 mg/L did not have obvious effects on liver function, glucose level, and circadian rhythm in larvae. However, Exposure to PBS, PHA, and PBAT at 1 mg/L could cause mild pathological injury of the liver and decreased glucose levels. Furthermore, exposure to PBS, PHA, and PBAT at 100 mg/L caused abnormal early development and pathological injury of the liver, increased ALT and TG levels, as well as decreased glucose levels. The molecular explanation of this was the variational expression levels of genes related to many aspects of biochemical pathways, such as oxidative stress, apoptosis, endoplasmic reticulum stress, fatty acid oxidation, and glucose metabolism. Meanwhile, larvae exposed to PBS, PHA, and PBAT at 100 mg/L showed chaos in circadian behaviors, accompanied by the disturbed expression of clock genes. Overall, we observed a greater adverse effect of PBS, PHA, and PBAT relative to PLA and PGA when we compared the effects induced by five MPs at the same exposure concentration. Our study provided important data to evaluate the ecological risk of new biodegradable polymers.</div></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"37 ","pages":"Article 100546"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143123386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}