NanoImpact最新文献

{"title":"Do nanoplastics impact Pb up-taking by Hordeum vulgare L.?","authors":"Nataliia Ryzhenko ,&nbsp;Lionel Dutruch ,&nbsp;Briscine Tabo ,&nbsp;Guillaume Pecheul ,&nbsp;Maxime Pattier ,&nbsp;Imane Khatib ,&nbsp;Mathieu Pédrot ,&nbsp;Julien Gigault ,&nbsp;Francisco Cabello-Hurtado ,&nbsp;Abdelhak El Amrani ,&nbsp;Mélanie Davranche","doi":"10.1016/j.impact.2024.100526","DOIUrl":"10.1016/j.impact.2024.100526","url":null,"abstract":"<div><p>Most studies on nanoplastics (NPs) focus on aquatic environments, overlooking their combined bioaccumulation with pollutants in terrestrial ecosystems. This study addresses a part of this gap by investigating how polystyrene nanoplastics (PS-NPs) affect the bioaccumulation and translocation of lead (Pb) in <em>Hordeum vulgare</em> L. plants. Using the RHIZOtest device for precise soil contamination control, we quantified PS-NPs (50 nm) in plant shoots via pyrolysis-gas chromatography/mass spectrometry (Py-GCMS) after plant KOH digestion. Our findings revealed that PS-NPs reduce Pb bioaccumulation and make adsorbed Pb onto PS-NPs less bioavailable to plants. For the highest Pb concentration, the Pb uptake index (PUI) followed the trend: Free Pb &gt; NPs + Pb &gt; Pb primary adsorbed by NPs, showing reduced Pb translocation to shoots in the presence of PS-NPs. Moreover, the presence of Pb decreased the bioavailability of PS-NPs probably in response to PS-NPs aggregation or modified charge. The PS-NPs concentrations in shoots range from 275.2 to 400 μg g⁻¹, representing 3.9 to 5.75% of the total PS-NPs. This study highlights the intricate interactions between nanoplastics and metals in soil-plant systems and emphasizes the need for further research on their combined effects and potential risks to food safety.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100526"},"PeriodicalIF":4.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of in vitro-in vivo extrapolation approaches for the risk assessment of nanomaterial toxicity","authors":"Rahmasari Nur Azizah ,&nbsp;Geert R. Verheyen ,&nbsp;Ziv Shkedy ,&nbsp;Sabine Van Miert","doi":"10.1016/j.impact.2024.100524","DOIUrl":"10.1016/j.impact.2024.100524","url":null,"abstract":"<div><p>Nanomaterials are increasingly used in many applications due to their enhanced properties. To ensure their safety for humans and the environment, nanomaterials need to be evaluated for their potential risk. The risk assessment analysis on the nanomaterials based on animal or <em>in vivo</em> studies is accompanied by several concerns, including animal welfare, time and cost needed for the studies. Therefore, incorporating <em>in vitro</em> studies in the risk assessment process is increasingly considered. To be able to analyze the potential risk of nanomaterial to human health, there are factors to take into account. Utilizing <em>in vitro</em> data in the risk assessment analysis requires methods that can be used to translate <em>in vitro</em> data to predict <em>in vivo</em> phenomena (<em>in vitro</em>-<em>in vivo</em> extrapolation (IVIVE) methods) to be incorporated, to obtain a more accurate result. Apart from the experiments and species conversion (for example, translation between the cell culture, animal and human), the challenge also includes the unique properties of nanomaterials that might cause them to behave differently compared to the same materials in a bulk form. This overview presents the IVIVE techniques that are developed to extrapolate pharmacokinetics data or doses. A brief example of the IVIVE methods for chemicals is provided, followed by a more detailed summary of available IVIVE methods applied to nanomaterials. The IVIVE techniques discussed include the comparison between <em>in vitro</em> and <em>in vivo</em> studies, methods to rene the dose metric or the <em>in vitro</em> models, allometric approach, mechanistic modeling, Multiple-Path Particle Dosimetry (MPPD), methods using organ burden data and also approaches that are currently being developed.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100524"},"PeriodicalIF":4.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A modified methodology for extraction and quantification of microplastics in soil","authors":"Ekta Gupta ,&nbsp;Virendra Kumar Mishra ,&nbsp;Anju Patel ,&nbsp;Pankaj Kumar Srivastava","doi":"10.1016/j.impact.2024.100525","DOIUrl":"10.1016/j.impact.2024.100525","url":null,"abstract":"<div><p>The ubiquitousness of microplastics (&lt;5 mm) has become a pressing environmental concern globally due to the extensive use of plastics. Microplastics have been well-studied in aquatic environments but not well-characterized in soils. Present analytical processes to quantify microplastics accurately in soil samples are quite challenging and require improved and validated analytical steps to eliminate the obscurities and biases. We aimed to develop an effective method for the extraction and quantification of microplastics from soil samples. Different ratios of low-(NaCl) and high-density solutions (ZnCl₂/ NaBr) were tested to determine the most efficient combination for density-dependent separation of microplastics from soil. The combination of low- (1:6) and high-density (1:3) solutions {as weight of soil(g)/volume of density solution(ml)} accounted for 95% recovery of the spiked microplastic particles from soil samples. Likewise, different soil-to-solution ratios of H₂O₂ were tested for the removal of soil organic matter with heating and non-heating steps. Prior removal of organic matter from soil samples achieved a clear supernatant that facilitated 99% recovery of microplastic particles. The validation of individually spiked microplastic particles of small (10-100 μm) and large scale (100-5000 μm) resulted in recovery ranging from 88 to 99%. A validated modified method with prior digestion followed by density-dependent separation was further tested using the field samples with microplastic contamination. The microplastics of different shapes, sizes, colours and polymeric compositions were reported efficiently and well characterized in the field-collected soil samples using this method.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100525"},"PeriodicalIF":4.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141971442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the dichloro-dihydro-fluorescein (DCFH) assay for the assessment of intracellular reactive oxygen species formation by nanomaterials","authors":"Nienke Ruijter ,&nbsp;Margriet van der Zee ,&nbsp;Alberto Katsumiti ,&nbsp;Matthew Boyles ,&nbsp;Flemming R. Cassee ,&nbsp;Hedwig Braakhuis","doi":"10.1016/j.impact.2024.100521","DOIUrl":"10.1016/j.impact.2024.100521","url":null,"abstract":"<div><p>To facilitate Safe and Sustainable by Design (SSbD) strategies during the development of nanomaterials (NMs), quick and easy <em>in vitro</em> assays to test for hazard potential at an early stage of NM development are essential. The formation of reactive oxygen species (ROS) and the induction of oxidative stress are considered important mechanisms that can lead to NM toxicity. <em>In vitro</em> assays measuring oxidative stress are therefore commonly included in NM hazard assessment strategies. The fluorescence-based dichloro-dihydro-fluorescein (DCFH) assay for cellular oxidative stress is a simple and cost-effective assay, making it a good candidate assay for SSbD hazard testing strategies. It is however subject to several pitfalls and caveats. Here, we provide further optimizations to the assay using 5-(6)-Chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H₂DCFDA-AE, referred to as DCFH probe), known for its improved cell retention.</p><p>We measured the release of metabolic products of the DCFH probe from cells to supernatant, direct reactions of CM-H₂DCFDA-AE with positive controls, and compared the commonly used plate reader-based DCFH assay protocol with fluorescence microscopy and flow cytometry-based protocols. After loading cells with DCFH probe, translocation of several metabolic products of the DCFH probe to the supernatant was observed in multiple cell types. Translocated DCFH products are then able to react with test substances including positive controls. Our results also indicate that intracellularly oxidized fluorescent DCF is able to translocate from cells to the supernatant. In either way, this will lead to a fluorescent supernatant, making it difficult to discriminate between intra- and extra-cellular ROS production, risking misinterpretation of possible oxidative stress when measuring fluorescence on a plate reader.</p><p>The use of flow cytometry instead of plate reader-based measurements resolved these issues, and also improved assay sensitivity. Several optimizations of the flow cytometry-based DCFH ISO standard (ISO/TS 19006:2016) were suggested, including loading cells with DCFH probe before incubation with the test materials, and applying an appropriate gating strategy including live-death staining, which was not included in the ISO standard.</p><p>In conclusion, flow cytometry- and fluorescence microscopy-based read-outs are preferred over the classical plate reader-based read-out to assess the level of intracellular oxidative stress using the cellular DCFH assay.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100521"},"PeriodicalIF":4.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000314/pdfft?md5=c9b29be2e74b40b7fe022bdcac03e48c&pid=1-s2.0-S2452074824000314-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141432330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Past progress in environmental nanoanalysis and a future trajectory for atomic mass-spectrometry methods","authors":"M.D. Montaño ,&nbsp;A.J. Goodman ,&nbsp;J.F. Ranville","doi":"10.1016/j.impact.2024.100518","DOIUrl":"10.1016/j.impact.2024.100518","url":null,"abstract":"<div><p>The development of engineered nanotechnology has necessitated a commensurate maturation of nanoanalysis capabilities. Building off a legacy established by electron microscopy and light-scattering, environmental nanoanalysis has now benefited from ongoing advancements in instrumentation and data analysis, which enable a deeper understanding of nanomaterial properties, behavior, and impacts. Where once environmental nanoparticles and colloids were grouped into broad ‘dissolved or particulate’ classes that are dependent on a filter size cut-off, now size distributions of submicron particles can be separated and characterized providing a more comprehensive examination of the nanoscale. Inductively coupled plasma-quadrupole mass spectrometry (ICP-QMS), directly coupled to field flow fractionation (FFF-ICP-QMS) or operated in single particle mode (spICP-MS) have spearheaded a revolution in nanoanalysis, enabling research into nanomaterial behavior in environmental and biological systems at expected release concentrations. However, the complexity of the nanoparticle population drives a need to characterize and quantify the multi-element composition of nanoparticles, which has begun to be realized through the application of time-of-flight MS (spICP-TOFMS). Despite its relative infancy, this technique has begun to make significant strides in more fully characterizing particulate systems and expanding our understanding of nanoparticle behavior. Though there is still more work to be done with regards to improving instrumentation and data processing, it is possible we are on the cusp of a new nanoanalysis revolution, capable of broadening our understanding of the size regime between dissolved and bulk particulate compartments of the environment.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100518"},"PeriodicalIF":4.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000284/pdfft?md5=57cd8ba86644dcc716c418e7ba0b27f4&pid=1-s2.0-S2452074824000284-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next Generation Risk Assessment approaches for advanced nanomaterials: Current status and future perspectives","authors":"Danail Hristozov ,&nbsp;Elena Badetti ,&nbsp;Paolo Bigini ,&nbsp;Andrea Brunelli ,&nbsp;Susan Dekkers ,&nbsp;Luisa Diomede ,&nbsp;Shareen H. Doak ,&nbsp;Wouter Fransman ,&nbsp;Agnieszka Gajewicz-Skretna ,&nbsp;Elisa Giubilato ,&nbsp;Laura Gómez-Cuadrado ,&nbsp;Roland Grafström ,&nbsp;Arno C. Gutleb ,&nbsp;Sabina Halappanavar ,&nbsp;Roland Hischier ,&nbsp;Neil Hunt ,&nbsp;Alberto Katsumiti ,&nbsp;Ali Kermanizadeh ,&nbsp;Antonio Marcomini ,&nbsp;Elisa Moschini ,&nbsp;Alex Zabeo","doi":"10.1016/j.impact.2024.100523","DOIUrl":"10.1016/j.impact.2024.100523","url":null,"abstract":"<div><p>This manuscript discusses the challenges of applying New Approach Methodologies (NAMs) for safe by design and regulatory risk assessment of advanced nanomaterials (AdNMs). The authors propose a framework for Next Generation Risk Assessment of AdNMs involving NAMs that is aligned to the conventional risk assessment paradigm. This framework is exposure-driven, endpoint-specific, makes best use of pre-existing information, and can be implemented in tiers of increasing specificity and complexity of the adopted NAMs. The tiered structure of the approach, which effectively combines the use of existing data with targeted testing will allow safety to be assessed cost-effectively and as far as possible with even more limited use of vertebrates. The regulatory readiness of state-of-the-art emerging NAMs is assessed in terms of Transparency, Reliability, Accessibility, Applicability, Relevance and Completeness, and their appropriateness for AdNMs is discussed in relation to each step of the risk assessment paradigm along with providing perspectives for future developments in the respective scientific and regulatory areas.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100523"},"PeriodicalIF":4.7,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000338/pdfft?md5=b6feab203d19e28d8b0aafbc6ce5d6d7&pid=1-s2.0-S2452074824000338-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation of nanoparticles during accelerated UV degradation of fleece polyester textiles","authors":"Tong Yang ,&nbsp;Bernd Nowack","doi":"10.1016/j.impact.2024.100520","DOIUrl":"10.1016/j.impact.2024.100520","url":null,"abstract":"<div><p>Micro- and nanoplastics have emerged as critical pollutants in various ecosystems, posing potential environmental and human health risks. Washing of polyester textiles has been identified as one of the sources of nanoplastics. However, other stages of the textile life cycle may also release nanoparticles. This study aimed to examine nanoparticle release during UV degradation of polyester textiles under controlled and real-world conditions. Fleece polyester textiles were weathered under simulated sunlight for up to two months, either in air or submerged in water. We conducted bi-weekly SEM image analyses and quantified released nanoparticles using nanoparticle tracking analysis (NTA). At week 0, the fiber surface appeared smooth after prewashing. In the air group, nanoparticles appeared on the fiber surface after UV-exposure. In the group of textiles submerged in water, the surfaces developed more pits over time. The cumulative nanoparticle emission from the weathered textiles ranged from 1.4 × 10¹¹ to 4.0 × 10¹¹ particles per gram of fabric in the air group and from 1.6 × 10¹¹ to 4.4 × 10¹¹ particles per gram of fabric in the water group over two months. The predominant particle size fell into the 100 to 200 nm range. The estimated mass of the released nanoparticles was 0.06–0.26 g per gram of fabric, which is lower than the amount released during the washing of new textiles. Additionally, Scanning Transmission X-ray Microscopy (STXM) images indicated that the weathered nanoparticles underwent oxidation. Overall, the research offers valuable insights into nanoparticle formation and release from polyester textiles during UV degradation.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100520"},"PeriodicalIF":4.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000302/pdfft?md5=69e210c2dfe29ad7919d1dfaa6a20232&pid=1-s2.0-S2452074824000302-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normalised similarity assessment to inform grouping of advanced multi-component nanomaterials by means of an Asymmetric Sigmoid function","authors":"Alex Zabeo ,&nbsp;Georgia Tsiliki ,&nbsp;Andrea Brunelli ,&nbsp;Elena Badetti ,&nbsp;José Balbuena ,&nbsp;Danail Hristozov","doi":"10.1016/j.impact.2024.100519","DOIUrl":"https://doi.org/10.1016/j.impact.2024.100519","url":null,"abstract":"<div><p>This manuscript presents a procedure for similarity assessment as a basis for grouping of multi component nanomaterials (MCNMs). This methodology is an adaptation of the approach by Zabeo et al. (2022), which includes an impactful change: the calculated similarities are normalised in the [0,1] domain by means of asymmetric Logistic scaling to simplify comparisons among properties' distances. This novel approach allows for grouping of nanomaterials that is not affected by the dataset, so that group membership will not change when new candidates are included in the set of assessed materials. It can be applied to assess groups of MCNMs as well as mixed groups of multi and single component nanomaterials as well as chemicals. To facilitate the application of the proposed methodology, a software script was developed by using the Python programming language, which is currently undergoing migration to a user-friendly web-based tool. The presented approach was tested against a real industrial case study provided by the Andalusian Innovation Centre for Sustainable Solution (CIAC): SiO₂-ZnO hybrid nanocomposite used in building coatings, which is designed to facilitate photocatalytic removal of NOx gases from the atmosphere. The results of applying the methodology in the case study demonstrated that ZnO is dissimilar from the other candidates mainly due to its different dissolution profiles.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100519"},"PeriodicalIF":4.9,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141429159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cellular response and molecular mechanism of superoxide dismutase interacting with superparamagnetic iron oxide nanoparticles","authors":"Hao Ju ,&nbsp;Yue Liu ,&nbsp;Yameng Wang ,&nbsp;Rui Lu ,&nbsp;Bin Yang ,&nbsp;Deyi Wang ,&nbsp;Jing Wang","doi":"10.1016/j.impact.2024.100515","DOIUrl":"10.1016/j.impact.2024.100515","url":null,"abstract":"<div><p>This study explored the response of superoxide dismutase (SOD) under superparamagnetic iron oxide nanoparticles (SPIONs)-induced oxidative stress using combined cellular and molecular methods. Results found that SPIONs induced the inhibition of catalase activity, the U-inverted change of SOD activity and the accumulation of reactive oxygen species (ROS), leading to oxidative damage and cytotoxicity. The change of intracellular SOD activity was resulted from the increase of molecular activity induced by directly interacting with SPIONs and ROS-inhibition of activity. The increase of molecular activity could be attributed to the structural and conformational changes of SOD, which were caused by the direct interaction of SOD with SPIONs. The SOD-SPIONs interaction and its interacting mechanism were explored by multi-spectroscopy, isothermal titration calorimetry and zeta potential assays. SOD binds to SPIONs majorly via hydrophobic forces with the involvement of electrostatic forces. SPIONs approximately adsorb 11 units of SOD molecule with the binding affinity of 2.99 × 10⁶ M⁻¹. The binding sites on SOD were located around Tyr residues, whose hydrophilicity increased upon interacting with SPIONs. The binding to SPIONs loosened the peptide chains, changed the secondary structure and reduced the aggregation state of SOD.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100515"},"PeriodicalIF":4.9,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study","authors":"Graziela de P.F. Dantas ,&nbsp;Fausto S. Ferraz ,&nbsp;John L.P. Coimbra ,&nbsp;Roberto M. Paniago ,&nbsp;Maria S.S. Dantas ,&nbsp;Samyra M.S.N. Lacerda ,&nbsp;Marcela S. Procópio ,&nbsp;Matheus F. Gonçalves ,&nbsp;Marcelo H. Furtado ,&nbsp;Bárbara P. Mendes ,&nbsp;Jorge L. López ,&nbsp;Alisson C. Krohling ,&nbsp;Estefânia M.N. Martins ,&nbsp;Lídia M. Andrade ,&nbsp;Luiz O. Ladeira ,&nbsp;Ângela L. Andrade ,&nbsp;Guilherme M.J. Costa","doi":"10.1016/j.impact.2024.100517","DOIUrl":"10.1016/j.impact.2024.100517","url":null,"abstract":"<div><p>Superparamagnetic iron oxide nanoparticles (SPIONs) have gained significant attention in biomedical research due to their potential applications. However, little is known about their impact and toxicity on testicular cells. To address this issue, we conducted an <em>in vitro</em> study using primary mouse testicular cells, testis fragments, and sperm to investigate the cytotoxic effects of sodium citrate-coated SPIONs (Cit_SPIONs). Herein, we synthesized and physiochemically characterized the Cit_SPIONs and observed that the sodium citrate diminished the size and improved the stability of nanoparticles in solution during the experimental time. The sodium citrate (measured by thermogravimetry) was biocompatible with testicular cells at the used concentration (3%). Despite these favorable physicochemical properties, the <em>in vitro</em> experiments demonstrated the cytotoxicity of Cit_SPIONs, particularly towards testicular somatic cells and sperm cells. Transmission electron microscopy analysis confirmed that Leydig cells preferentially internalized Cit_SPIONs in the organotypic culture system, which resulted in alterations in their cytoplasmic size. Additionally, we found that Cit_SPIONs exposure had detrimental effects on various parameters of sperm cells, including motility, viability, DNA integrity, mitochondrial activity, lipid peroxidation (LPO), and ROS production. Our findings suggest that testicular somatic cells and sperm cells are highly sensitive and vulnerable to Cit_SPIONs and induced oxidative stress. This study emphasizes the potential toxicity of SPIONs, indicating significant threats to the male reproductive system. Our findings highlight the need for detailed development of iron oxide nanoparticles to enhance reproductive nanosafety.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"35 ","pages":"Article 100517"},"PeriodicalIF":4.9,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}