NanoImpact最新文献

{"title":"Formation of nanoparticles during accelerated UV degradation of fleece polyester textiles","authors":"Tong Yang ,&nbsp;Bernd Nowack","doi":"10.1016/j.impact.2024.100520","DOIUrl":"10.1016/j.impact.2024.100520","url":null,"abstract":"<div><p>Micro- and nanoplastics have emerged as critical pollutants in various ecosystems, posing potential environmental and human health risks. Washing of polyester textiles has been identified as one of the sources of nanoplastics. However, other stages of the textile life cycle may also release nanoparticles. This study aimed to examine nanoparticle release during UV degradation of polyester textiles under controlled and real-world conditions. Fleece polyester textiles were weathered under simulated sunlight for up to two months, either in air or submerged in water. We conducted bi-weekly SEM image analyses and quantified released nanoparticles using nanoparticle tracking analysis (NTA). At week 0, the fiber surface appeared smooth after prewashing. In the air group, nanoparticles appeared on the fiber surface after UV-exposure. In the group of textiles submerged in water, the surfaces developed more pits over time. The cumulative nanoparticle emission from the weathered textiles ranged from 1.4 × 10¹¹ to 4.0 × 10¹¹ particles per gram of fabric in the air group and from 1.6 × 10¹¹ to 4.4 × 10¹¹ particles per gram of fabric in the water group over two months. The predominant particle size fell into the 100 to 200 nm range. The estimated mass of the released nanoparticles was 0.06–0.26 g per gram of fabric, which is lower than the amount released during the washing of new textiles. Additionally, Scanning Transmission X-ray Microscopy (STXM) images indicated that the weathered nanoparticles underwent oxidation. Overall, the research offers valuable insights into nanoparticle formation and release from polyester textiles during UV degradation.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.7,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000302/pdfft?md5=69e210c2dfe29ad7919d1dfaa6a20232&pid=1-s2.0-S2452074824000302-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141437247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normalised similarity assessment to inform grouping of advanced multi-component nanomaterials by means of an Asymmetric Sigmoid function","authors":"Alex Zabeo ,&nbsp;Georgia Tsiliki ,&nbsp;Andrea Brunelli ,&nbsp;Elena Badetti ,&nbsp;José Balbuena ,&nbsp;Danail Hristozov","doi":"10.1016/j.impact.2024.100519","DOIUrl":"https://doi.org/10.1016/j.impact.2024.100519","url":null,"abstract":"<div><p>This manuscript presents a procedure for similarity assessment as a basis for grouping of multi component nanomaterials (MCNMs). This methodology is an adaptation of the approach by Zabeo et al. (2022), which includes an impactful change: the calculated similarities are normalised in the [0,1] domain by means of asymmetric Logistic scaling to simplify comparisons among properties' distances. This novel approach allows for grouping of nanomaterials that is not affected by the dataset, so that group membership will not change when new candidates are included in the set of assessed materials. It can be applied to assess groups of MCNMs as well as mixed groups of multi and single component nanomaterials as well as chemicals. To facilitate the application of the proposed methodology, a software script was developed by using the Python programming language, which is currently undergoing migration to a user-friendly web-based tool. The presented approach was tested against a real industrial case study provided by the Andalusian Innovation Centre for Sustainable Solution (CIAC): SiO₂-ZnO hybrid nanocomposite used in building coatings, which is designed to facilitate photocatalytic removal of NOx gases from the atmosphere. The results of applying the methodology in the case study demonstrated that ZnO is dissimilar from the other candidates mainly due to its different dissolution profiles.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141429159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cellular response and molecular mechanism of superoxide dismutase interacting with superparamagnetic iron oxide nanoparticles","authors":"Hao Ju ,&nbsp;Yue Liu ,&nbsp;Yameng Wang ,&nbsp;Rui Lu ,&nbsp;Bin Yang ,&nbsp;Deyi Wang ,&nbsp;Jing Wang","doi":"10.1016/j.impact.2024.100515","DOIUrl":"10.1016/j.impact.2024.100515","url":null,"abstract":"<div><p>This study explored the response of superoxide dismutase (SOD) under superparamagnetic iron oxide nanoparticles (SPIONs)-induced oxidative stress using combined cellular and molecular methods. Results found that SPIONs induced the inhibition of catalase activity, the U-inverted change of SOD activity and the accumulation of reactive oxygen species (ROS), leading to oxidative damage and cytotoxicity. The change of intracellular SOD activity was resulted from the increase of molecular activity induced by directly interacting with SPIONs and ROS-inhibition of activity. The increase of molecular activity could be attributed to the structural and conformational changes of SOD, which were caused by the direct interaction of SOD with SPIONs. The SOD-SPIONs interaction and its interacting mechanism were explored by multi-spectroscopy, isothermal titration calorimetry and zeta potential assays. SOD binds to SPIONs majorly via hydrophobic forces with the involvement of electrostatic forces. SPIONs approximately adsorb 11 units of SOD molecule with the binding affinity of 2.99 × 10⁶ M⁻¹. The binding sites on SOD were located around Tyr residues, whose hydrophilicity increased upon interacting with SPIONs. The binding to SPIONs loosened the peptide chains, changed the secondary structure and reduced the aggregation state of SOD.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The toxicity of superparamagnetic iron oxide nanoparticles induced on the testicular cells: In vitro study","authors":"Graziela de P.F. Dantas ,&nbsp;Fausto S. Ferraz ,&nbsp;John L.P. Coimbra ,&nbsp;Roberto M. Paniago ,&nbsp;Maria S.S. Dantas ,&nbsp;Samyra M.S.N. Lacerda ,&nbsp;Marcela S. Procópio ,&nbsp;Matheus F. Gonçalves ,&nbsp;Marcelo H. Furtado ,&nbsp;Bárbara P. Mendes ,&nbsp;Jorge L. López ,&nbsp;Alisson C. Krohling ,&nbsp;Estefânia M.N. Martins ,&nbsp;Lídia M. Andrade ,&nbsp;Luiz O. Ladeira ,&nbsp;Ângela L. Andrade ,&nbsp;Guilherme M.J. Costa","doi":"10.1016/j.impact.2024.100517","DOIUrl":"10.1016/j.impact.2024.100517","url":null,"abstract":"<div><p>Superparamagnetic iron oxide nanoparticles (SPIONs) have gained significant attention in biomedical research due to their potential applications. However, little is known about their impact and toxicity on testicular cells. To address this issue, we conducted an <em>in vitro</em> study using primary mouse testicular cells, testis fragments, and sperm to investigate the cytotoxic effects of sodium citrate-coated SPIONs (Cit_SPIONs). Herein, we synthesized and physiochemically characterized the Cit_SPIONs and observed that the sodium citrate diminished the size and improved the stability of nanoparticles in solution during the experimental time. The sodium citrate (measured by thermogravimetry) was biocompatible with testicular cells at the used concentration (3%). Despite these favorable physicochemical properties, the <em>in vitro</em> experiments demonstrated the cytotoxicity of Cit_SPIONs, particularly towards testicular somatic cells and sperm cells. Transmission electron microscopy analysis confirmed that Leydig cells preferentially internalized Cit_SPIONs in the organotypic culture system, which resulted in alterations in their cytoplasmic size. Additionally, we found that Cit_SPIONs exposure had detrimental effects on various parameters of sperm cells, including motility, viability, DNA integrity, mitochondrial activity, lipid peroxidation (LPO), and ROS production. Our findings suggest that testicular somatic cells and sperm cells are highly sensitive and vulnerable to Cit_SPIONs and induced oxidative stress. This study emphasizes the potential toxicity of SPIONs, indicating significant threats to the male reproductive system. Our findings highlight the need for detailed development of iron oxide nanoparticles to enhance reproductive nanosafety.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probabilistic material flow analysis of released nano titanium dioxide in Mexico","authors":"Luis Mauricio Ortiz-Galvez ,&nbsp;Alejandro Caballero-Guzman ,&nbsp;Carla Lopes ,&nbsp;Ernesto Alfaro-Moreno","doi":"10.1016/j.impact.2024.100516","DOIUrl":"10.1016/j.impact.2024.100516","url":null,"abstract":"<div><p>Engineered Nanomaterials (ENMs) or products containing ENMs, known as nano-enabled products are commercialized globally by a large number of companies. Concern about the potential risks and negative impacts of releasing ENMs into the environment is under investigation. For this reason, methodologies to estimate the probable mass concentrations of ENMs released in different regions of the world have been developed. As a first attempt to estimate the probable mass flows of nanosized titanium dioxide (nano-TiO₂) released in Mexico, we developed a Probabilistic Material Flow Analysis (PMFA) for 2015.</p><p>The model describes probabilistic mass flows of released nano-TiO₂ during the life cycle of sunscreens, coatings, ceramic, and other nano-enabled products, including the flows through the solid waste and wastewater management systems, as well as the transfer of nano-TiO₂ to three environmental compartments (atmosphere, topsoil, and surface water). The PMFA incorporates the uncertainty related to the input data. We observed that the most significant nano-TiO₂ flows occur to the surface water, landfill, and soil compartments, targeted as the main “hot-spots”, where living organisms could be more exposed to this material. Further improvements in the model are needed due to some data gaps at some life cycle stages, for instance, solid waste management and reused wastewater manipulation for irrigation purposes. Finally, the model developed in this study can be adjusted to assess other ENM releases and can be beneficial for further investigation in fate modeling and environmental risk assessment.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141262208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Governance of advanced materials: Shaping a safe and sustainable future","authors":"Monique Groenewold ,&nbsp;Eric A.J. Bleeker ,&nbsp;Cornelle W. Noorlander ,&nbsp;Adriënne J.A.M. Sips ,&nbsp;Margriet van der Zee ,&nbsp;Robert J. Aitken ,&nbsp;James H. Baker ,&nbsp;Martine I. Bakker ,&nbsp;Evert A. Bouman ,&nbsp;Shareen H. Doak ,&nbsp;Damjana Drobne ,&nbsp;Verónica I. Dumit ,&nbsp;Marie-Valentine Florin ,&nbsp;Wouter Fransman ,&nbsp;Mar M. Gonzalez ,&nbsp;Elisabeth Heunisch ,&nbsp;Panagiotis Isigonis ,&nbsp;Nina Jeliazkova ,&nbsp;Keld Alstrup Jensen ,&nbsp;Thomas Kuhlbusch ,&nbsp;Janeck J. Scott-Fordsmand","doi":"10.1016/j.impact.2024.100513","DOIUrl":"10.1016/j.impact.2024.100513","url":null,"abstract":"<div><p>The past few decades of managing the uncertain risks associated with nanomaterials have provided valuable insights (knowledge gaps, tools, methods, etc.) that are equally important to promote safe and sustainable development and use of advanced materials. Based on these insights, the current paper proposes several actions to optimize the risk and sustainability governance of advanced materials.</p><p>We emphasise the importance of establishing a European approach for risk and sustainability governance of advanced materials as soon as possible to keep up with the pace of innovation and to manage uncertainty among regulators, industry, SMEs and the public, regarding potential risks and impacts of advanced materials. Coordination of safe and sustainable advanced material research efforts, and data management according to the Findable, Accessible, Interoperable and Reusable (FAIR) principles will enhance the generation of regulatory-relevant knowledge. This knowledge is crucial to identify whether current regulatory standardised and harmonised test methods are adequate to assess advanced materials. At the same time, there is urgent need for responsible innovation beyond regulatory compliance which can be promoted through the Safe and Sustainable Innovation Approach. that combines the Safe and Sustainable by Design concept with Regulatory Preparedness, supported by a trusted environment. We further recommend consolidating all efforts and networks related to the risk and sustainability governance of advanced materials in a single, easy-to-use digital portal.</p><p>Given the anticipated complexity and tremendous efforts required, we identified the need of establishing an organisational structure dedicated to aligning the fast technological developments in advanced materials with proper risk and sustainability governance. Involvement of multiple stakeholders in a trusted environment ensures a coordinated effort towards the safe and sustainable development, production, and use of advanced materials. The existing infrastructures and network of experts involved in the governance of nanomaterials would form a solid foundation for such an organisational structure.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141184166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical methods for quantifying PS and PVC Nanoplastic attachment to activated sludge Bacteria and their impact on community structure","authors":"Fazel Abdolahpur Monikh ,&nbsp;Nhung H.A. Nguyen ,&nbsp;Mandar Bandekar ,&nbsp;Jakub Riha ,&nbsp;Sara Bogialli ,&nbsp;Paolo Pastore ,&nbsp;Hans-Peter Grossart ,&nbsp;Alena Sevcu","doi":"10.1016/j.impact.2024.100514","DOIUrl":"10.1016/j.impact.2024.100514","url":null,"abstract":"<div><p>Nanoplastics are anticipated to be ubiquitous in various environmental compartments. However, challenges in analytical methods hinder our understanding of risks related to specific nanplastics characteristics such as size and chemical compositions, and interactions between nanoplastics and microorganisms. In this study, we applied fit-for-purpose analytical methods and techniques to understand how nanoplastic chemical composition influences their interaction with bacteria collected from activated sludge. When exposed to polystyrene (PS) and polyvinyl chloride (PVC) nanoplastics for 5 days, the nanoplastics attached to the bacteria. Specifically, on day 1, there was a significant predominance of PS nanoplastics over PVC ones of similar size and shape, possibly due to differences in their chemical composition. After 5 days, there is a substantial decrease in nanoplastics attached to bacteria, suggesting bacterial defence mechanisms may reduce particles attachment over time. The overall bacterial community structure demonstrated a high degree of resilience. This resilience highlights the ability of microbial communities to maintain their structure despite nanoplastic stressors, as evidenced by consistent alpha diversity, PCoA, and PERMANOVA results. Understanding these mechanisms is crucial for assessing nanoplastic fate and thus environmental impacts.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000247/pdfft?md5=e2327df1c115cfa349a1366b2d6a9b3b&pid=1-s2.0-S2452074824000247-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141184162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"(Re)imagining purpose: A framework for sustainable nanotechnology innovation","authors":"Paris Jeffcoat ,&nbsp;Cary Di Lernia ,&nbsp;Catherine Hardy ,&nbsp;Elizabeth J. New ,&nbsp;Wojciech Chrzanowski","doi":"10.1016/j.impact.2024.100511","DOIUrl":"10.1016/j.impact.2024.100511","url":null,"abstract":"<div><p>To fully understand and predict the impact of nanotechnologies, a truly multidisciplinary approach is required. However, the practicalities relating to <em>how</em> innovation, commercialisation, risk assessment, informatics, and governance in nanotechnology should intersect remain somewhat of a black box. To begin to shed light on this intersection, we identify a need to place ‘purpose’ at the heart of the nanotechnology innovation ecosystem. There is a growing appetite for responsible, sustainable, and purposeful innovation from business, financiers, regulators, consumers, and other stakeholders – an appetite that we foresee will permeate all spheres of commercialisation, including that of nanotechnology. Ultimately, nanotechnologies will only have the ability to sustainably address the global challenges of the 21st century if they are developed and implemented with purpose, and in full consideration of their social and environmental impacts. We (re)define purpose as it relates to sustainable nanotechnology innovation, in an effort to create a more-broadly shared language that can bridge the diverse stakeholder needs and perspectives that are required to address these challenges. To enable innovation, standardisation, promote interdisciplinarity, increase transparency, and enhance regulatory and corporate accountability, we propose a four stage, principles-based framework for purposeful nanotechnology development. This framework offers a practical way forward for nanotechnology innovation, shedding light on how nano-impact can be approached by multidisciplinary teams and describing how interrelated systems and stakeholders can interact successfully to achieve shared goals.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000211/pdfft?md5=3e744f248294742e3a2864173b8c04b3&pid=1-s2.0-S2452074824000211-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of micro(nano)plastics based on automated data interpretation and modeling: A review","authors":"Kwanyoung Ko ,&nbsp;Juhwan Lee ,&nbsp;Philipp Baumann ,&nbsp;Jaeho Kim ,&nbsp;Haegeun Chung","doi":"10.1016/j.impact.2024.100509","DOIUrl":"10.1016/j.impact.2024.100509","url":null,"abstract":"<div><p>The widespread presence of micro(nano)plastics (MNPs) in the environment threatens ecosystem integrity, and thus, it is necessary to determine and assess the occurrence, characteristics, and transport of MNPs between ecological components. However, most analytical approaches are cost- and time-inefficient in providing quantitative information with sufficient detail, and interpreting results can be difficult. Alternative analyses integrating novel measurements by imaging or proximal sensing with signal processing and machine learning may supplement these approaches. In this review, we examined published research on methods used for the automated data interpretation of MNPs found in the environment or those artificially prepared by fragmenting bulk plastics. We critically reviewed the primary areas of the integrated analytical process, which include sampling, data acquisition, processing, and modeling, applied in identifying, classifying, and quantifying MNPs in soil, sediment, water, and biological samples. We also provide a comprehensive discussion regarding model uncertainties related to estimating MNPs in the environment. In the future, the development of routinely applicable and efficient methods is expected to significantly contribute to the successful establishment of automated MNP monitoring systems.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140909587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Algal growth inhibition test with TEMPO-oxidized cellulose nanofibers","authors":"Rie Tai ,&nbsp;Isamu Ogura ,&nbsp;Toshiya Okazaki ,&nbsp;Yoko Iizumi ,&nbsp;Hiroyuki Mano","doi":"10.1016/j.impact.2024.100504","DOIUrl":"10.1016/j.impact.2024.100504","url":null,"abstract":"<div><p>Ecotoxicity data on cellulose nanofibers (CNFs) are limited despite their wide potential applications prospects, such as structural and packaging materials, filters, coatings, foods, and cosmetics. In this study, toxicity tests of 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-oxidized CNFs (TEMPO-CNFs), which are one of the major CNF products commercially available in Japan, on the green alga <em>Raphidocelis subcapitata</em> were conducted. As nanomaterials are considered difficult-to-test substances, the Organisation for Economic Co-operation and Development has released a guidance document that provides considerations regarding ecotoxicity tests of nanomaterials. In the algal growth inhibition tests of TEMPO-CNFs, there were specific issues to be examined, including the effects of medium components on the characteristics of TEMPO-CNFs, CNF interference with algal density measurements, algal interference with CNF measurements, and the effects of ion concentration changes in the test medium by the addition of CNFs on algal growth. To examine these issues, we conducted preliminary studies and established a suitable test method for algal growth inhibition tests of TEMPO-CNFs. We confirmed that the components in the medium for algal growth inhibition tests had negligible effects on the characteristics (zeta-potential, viscosity, and morphology) and concentration stability of TEMPO-CNFs and that <em>in vitro</em> and <em>in vivo</em> fluorescence measurements were applicable for estimating the algal densities, without interference by TEMPO-CNFs. In contrast, we observed that the grown algae interfered with the CNF concentration measurements. Therefore, we established a method to correct the measured CNF concentrations by estimating the algal contribution. Furthermore, we found that the nutrient salt concentrations in the medium changed due to interactions with CNFs; however, this change did not affect algal growth. Based on the results of the preliminary studies, algal growth inhibition tests of TEMPO-CNFs were conducted using <em>in vitro</em> and <em>in vivo</em> fluorescence measurements, along with measurements of CNFs and ion concentrations in the test dispersions. The test results showed that no growth inhibition was observed on growth rate or yield even at the maximum CNF concentration of 100 mg/L, suggesting that the ecological effect of TEMPO-CNFs on algae was relatively low. The results of this study will be valuable for conducting ecotoxicity assessments on additional CNFs and comparable nanomaterials in future studies.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452074824000144/pdfft?md5=b227c55da85d42da6c0fdd69ff9db24e&pid=1-s2.0-S2452074824000144-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140306158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}