NanoImpact最新文献

{"title":"Quantitative evaluation of released nanomaterials from carbon nanotube epoxy nanocomposites during environmental exposure and mechanical treatment","authors":"Yue Zhao ,&nbsp;David G. Goodwin Jr. ,&nbsp;Lipiin Sung ,&nbsp;Girish Ramakrishnan ,&nbsp;Qiyuan Wu ,&nbsp;Jiajie Cen ,&nbsp;Elijah J. Petersen ,&nbsp;Alexander Orlov","doi":"10.1016/j.impact.2023.100486","DOIUrl":"10.1016/j.impact.2023.100486","url":null,"abstract":"<div><p>Carbon nanotubes (CNTs) are promising nanomaterials exhibiting high thermal and electrical conductivities, significant stiffness, and high tensile strength. As a result, CNTs have been utilized as additives to enhance properties of various polymeric materials in a broad range of fields. In this study, we investigated the release of CNTs from CNT epoxy nanocomposites exposed to environmental weathering and mechanical stresses. The presence and amount of CNTs released from degraded polymer nanocomposites is important because CNTs can impact physiological systems in humans and environmental organisms. The weathering experiments in this study included nanocomposite exposure to both UV and a water spray, to simulate sunlight and rain exposure, whereas mechanical stresses were induced by shaking and ultrasonication. CNT release from epoxy nanocomposites was quantified by a ¹⁴C-labeling method that enabled measurement of the CNT release rates after different weathering and mechanical treatments. In this study, a sample oxidizer was used prior to liquid scintillation counting, because it was shown to reduce interferences from the presence of polymeric materials and achieve a high recovery (95%). Polymer nanocomposite degradation was confirmed by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and light microscopy. A continuous release of ¹⁴C-labeled nanomaterials was observed after each UV and simulated rain exposure period, with 0.23% (mass/mass) of the total embedded mass of CNTs being released from the CNT nanocomposite during the full weathering process, suggesting that the water spray induced sufficient mechanical stress to eliminate the protective effect of the surface agglomerated CNT network. Importantly, additional mechanical stresses imposed on the weathered nanocomposites by shaking and ultrasonication resulted in further release of approximately 0.27% (mass /mass).</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"32 ","pages":"Article 100486"},"PeriodicalIF":4.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41117804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A roadmap to strengthen standardisation efforts in risk governance of nanotechnology","authors":"Kirsten Rasmussen ,&nbsp;Eric A.J. Bleeker ,&nbsp;James Baker ,&nbsp;Jacques Bouillard ,&nbsp;Wouter Fransman ,&nbsp;Thomas A.J. Kuhlbusch ,&nbsp;Susanne Resch ,&nbsp;Jacques-Aurélien Sergent ,&nbsp;Lya G. Soeteman-Hernandez ,&nbsp;Blanca Suarez-Merino ,&nbsp;Andrea Porcari","doi":"10.1016/j.impact.2023.100483","DOIUrl":"10.1016/j.impact.2023.100483","url":null,"abstract":"<div><p>A roadmap was developed to strengthen standardisation activities for risk governance of nanotechnology. Its baseline is the available standardised and harmonised methods for nanotechnology developed by the International Organization for Standardization (ISO), the European Committee for Standardization (CEN), and the Organisation for Economic Co-operation and Development (OECD). In order to identify improvements and needs for new themes in standardisation work, an analysis of the state-of-the-art concepts and interpretations of risk governance of nanotechnology was performed. Eleven overall areas of action were identified, each including a subset of specific topics. Themes addressed include physical chemical characterisation, assessment of hazard, exposure, risk and socio-economic factors, as well as education &amp; training and social dialogue. This has been visualised in a standardisation roadmap spanning a timeframe of ten years and including key outcomes and highlights of the analysis. Furthermore, the roadmap indicates potential areas of action for harmonisation and standardisation (H&amp;S) for nanomaterials and nanotechnology. It also includes an evaluation of the current level (limited, moderate, intense) of ongoing H&amp;S activities and indicates the time horizon for the different areas of action. As the identified areas differ in their state of development, the number and type of actions varied widely amongst the different actions towards achieving standardisation. Thus, priority areas were also identified. The overall objective of these actions is to strengthen risk governance towards a safe use of nanomaterials and nano-related products. Though not explicitly addressed, risk-based legislation and policies are supported via the proposed H&amp;S actions.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"32 ","pages":"Article 100483"},"PeriodicalIF":4.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41125033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of a real food matrix and in vitro digestion on properties and acute toxicity of polystyrene microparticles","authors":"Nazım Sergen Mısırlı ,&nbsp;Wittaya Pimtong ,&nbsp;Siwapech Sillapaprayoon ,&nbsp;Varissara Chantho ,&nbsp;Rattaporn Saenmuangchin ,&nbsp;Sasitorn Aueviriyavit ,&nbsp;Fahriye Ceyda Dudak","doi":"10.1016/j.impact.2023.100482","DOIUrl":"10.1016/j.impact.2023.100482","url":null,"abstract":"<div><p>Although it is proved that humans ingest microplastics <em>via</em> food, and microplastics were found in human tissues, blood and feces, there needs to be more data on the properties and health-related effects of plastic particles that interact with food and undergo digestion. This study aimed to examine the impact of a real food matrix, milk, on the behavior and gastrointestinal fate of polystyrene microparticles (PSMP). In the presence of the food matrix, the net negative ζ-potential values of PSMP (diameter size of 1.823 μm) decreased significantly due to the formation of the corona, mostly consisting of α and β-casein fragments. Protein corona profiles and morphologies of particles incubated with whole and skim milk were found to be similar, and the protein profiles were completely altered after <em>in vitro</em> digestion simulation. <em>In vitro</em> and <em>in vivo</em> toxicity studies showed that neither bare PSMP nor food-interacted PSMP pose acute toxicity on the Caco-2 cell line and zebrafish embryos under the chosen experimental conditions. In summary, these results may contribute to a better understanding of changes that microplastics undergo in foods. Further studies on repeated exposure or chronic toxicity are needed to fully reveal the effect of food matrix on microplastic toxicity.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"32 ","pages":"Article 100482"},"PeriodicalIF":4.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10338561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"European nanomaterial legislation in the past 20 years – Closing the final gaps","authors":"Maria Bille Nielsen,&nbsp;Lars Skjolding,&nbsp;Anders Baun,&nbsp;Steffen Foss Hansen","doi":"10.1016/j.impact.2023.100487","DOIUrl":"10.1016/j.impact.2023.100487","url":null,"abstract":"<div><p>In 2004, the potential societal implications related to nanotechnology were highlighted in an influential report by the Royal Society and the Royal Academy of Engineering (RS &amp; RAE). It was made clear that legislation is an important tool to tackle the challenges related to nanomaterials and a list of recommendations were put forward. Shortly after, the European Commission also proposed a list of recommendations on how to handle nanomaterial challenges and adopted the so-called “incremental approach”, describing that current legislations should be adapted, where relevant, to handle nanomaterials. Now almost 20 years have passed and it seems relevant to take stock and investigate how legislations have been adapted to tackle nano-specific challenges. In this review, we analyze key pieces of European legislations relevant to nanomaterials and assess to what extent these legislations compare with the original recommendations from 2004 by the RS &amp; RAE and the European Commission. We uncover the cross-cutting challenges that remain and provide recommendations on next steps that should be taken to address the risks of nanomaterials. For each recommendation, we assessed whether it was met to a high, medium or low degree by conducting targeted literature searches at Web of Science, screening legislations, guidance documents, databases etc., and applying expert judgement. We found that &gt;90% of the recommendations put forward in 2004 by the RS &amp; RAE and the European Commission have been either met to a high degree (13 out of 29) or met to a medium degree (14 out of 29). This suggests important advancements in the field of nanosafety. At the same time, it is important to address the concerns still left partly or fully unsolved. Such efforts entail e.g. further development of measuring instruments and standardised characterization and risk assessment methods for nanomaterials, application of a uniform nanomaterial definition, maximization of containment of free nanomaterials until hazards assessed/handled and elimination/minimisation of unintentional nanomaterial emission. Furthermore, we recommend prioritising future efforts to ensure enforcement and implementation of existing nano-specific provisions, as well as revision, where needed, of legislations that currently do not account for nanomaterials, such as the Waste Framework Directive.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"32 ","pages":"Article 100487"},"PeriodicalIF":4.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41205342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palladium-doped and undoped polystyrene nanoplastics in a chronic toxicity test for higher plants: Impact on soil, plants and ammonium oxidizing bacteria","authors":"Martin Hoppe ,&nbsp;Jan Köser ,&nbsp;Georg Scheeder ,&nbsp;Axel Lamparter ,&nbsp;Kristof Dorau ,&nbsp;Lena Grüger ,&nbsp;Georg Dierkes ,&nbsp;Karsten Schlich","doi":"10.1016/j.impact.2023.100484","DOIUrl":"10.1016/j.impact.2023.100484","url":null,"abstract":"<div><p>There is a lack of knowledge about the fate and impact of microplastics (MPs) and nanoplastics (NPs), as well as their potential uptake and impact on plants and microorganisms. The predicted environmental concentrations (PEC) of frequent polymers in soils are low, and therefore, difficult to detect with the available techniques, which explains the knowledge gaps. Therefore, model particles (polystyrene particles (PS-P), 343 nm) and palladium (Pd) nanoparticle-doped polystyrene particles (PS-Pd-PS-P, 442 nm) were synthesized, characterized, and subsequently applied to agricultural soils (Cambisol, Podzol, PS target contents: 25 mg kg⁻¹, 75 mg kg⁻¹, 225 mg kg⁻¹). A combination of different techniques, such as inductively coupled plasma-mass spectrometry (ICP-MS), pyrolysis-gas chromatography–mass spectrometry (Pyr-GC–MS), dynamic light scattering (DLS), and scanning electron microscopy (SEM), were used to characterize the particles in the dispersions, soils and plants. The spiked soils were applied to a chronical plant toxicity test with oat (<em>Avena sativa</em>). The applied particle contents could be recovered from both soils by ICP-MS (Pd, 89% - 99%), and Pyr-GC–MS (PS, 73% - 120%). Moreover, non-aggregated particles in soils and on oat roots were visualized through SEM. The ratio obtained for the Pd contents in oat roots to that in the Cambisol (2.2–2.7) and the Podzol (2.3–2.6) implied that particles accumulated on the root surface or in the roots. No Pd was detected in the oat shoots, which indicated that no translocation occurred from the roots to the shoots. Despite particle accumulation at or in the roots, no clear effects on plant growth were observed. Furthermore, the soil microorganisms (Podzol) and the soil water repellency (Cambisol, Podzol) showed no clear monotone concentration-response relationship after exposure to PS-P and PS-Pd-PS-P. The findings are complex and illustrate the urgent need for further sophisticated experimental studies to elucidate the impacts of NPs on physicochemical soil function, plants, and soil organisms. The model PS-P doped with Pd nanoparticles significantly enhanced the development and validation of methods for investigating MPs and NPs in environmental matrices, highlighting their considerable potential for further studies.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"32 ","pages":"Article 100484"},"PeriodicalIF":4.9,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Release and toxicity assessment of carbon nanomaterial reinforced polymers during the use and end-of-life phases: A comparative review","authors":"Daina Romeo,&nbsp;Pietro Clement,&nbsp;Peter Wick","doi":"10.1016/j.impact.2023.100477","DOIUrl":"10.1016/j.impact.2023.100477","url":null,"abstract":"<div><p>The research on carbon-based nanomaterial (C-NM) composites has increased in the last two decades. This family of functional materials shows outstanding mechanical, thermal and electrical properties, and are being used in a variety of applications. An important challenge remains before C-NM can be fully integrated in our production industries and our lives: to assess the release of debris during production, use, and misuse of composites and the effect they may have on the environment and on human health. During their lifecycle, composites materials can be subjected to a variety of stresses which may release particles from the macroscopic range to the nanoscale. In this review, the release of debris due to abrasion, weathering and combustion as well as their toxicity is evaluated for the three most used C-NM: Carbon Black, Carbon Nanotubes and Graphene-related materials. The goal is to stimulate a Safe-By-Design approach by guiding the selection of carbon nano-fillers for specific applications based of safety and performance.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100477"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10135902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The in vitro immunomodulatory effect of multi-walled carbon nanotubes by multilayer analysis","authors":"Veera Hautanen ,&nbsp;Jack Morikka ,&nbsp;Laura Aliisa Saarimäki ,&nbsp;Jan Bisenberger ,&nbsp;Tarja Toimela ,&nbsp;Angela Serra ,&nbsp;Dario Greco","doi":"10.1016/j.impact.2023.100476","DOIUrl":"10.1016/j.impact.2023.100476","url":null,"abstract":"<div><p>The study of multi-walled carbon nanotube (MWCNT) induced immunotoxicity is crucial for determining hazards posed to human health. MWCNT exposure most commonly occurs via the airways, where macrophages are first line responders. Here we exploit an in vitro assay, measuring dose-dependent secretion of a wide panel of cytokines, as a measure of immunotoxicity following the non-lethal, multi-dose exposure (IC5, IC10 and IC20) to 7 MWCNTs with different intrinsic properties. We find that a tangled structure, and small aspect ratio are key properties predicting MWCNT induced immunotoxicity, mediated predominantly by IL1B cytokine secretion. To assess the mechanism of action giving rise to MWCNT immunotoxicity, transcriptomics analysis was linked to cytokine secretion in a multilayer model established through correlation analysis across exposure concentrations. This reinforced the finding that tangled MWCNTs have greater immunomodulatory potency, displaying enrichment of immune system, signal transduction and pattern recognition associated pathways. Together our results further elucidate how structure, length and aspect ratio, critical intrinsic properties of MWCNTs, are tied to immunotoxicity.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100476"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10140664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are nanoplastics potentially toxic for plants and rhizobiota? Current knowledge and recommendations","authors":"Delphine Masson ,&nbsp;Mathieu Pédrot ,&nbsp;Mélanie Davranche ,&nbsp;Francisco Cabello-Hurtado ,&nbsp;Nataliia Ryzhenko ,&nbsp;Abdelhak El Amrani ,&nbsp;Aurélie Wahl ,&nbsp;Julien Gigault","doi":"10.1016/j.impact.2023.100473","DOIUrl":"10.1016/j.impact.2023.100473","url":null,"abstract":"<div><p>Soil is now becoming a reservoir of plastics in response to global production, use/disposal patterns and low recovery rates. Their degradation is caused by numerous processes, and this degradation leads to the formation and release of plastic nanoparticles, i.e., nanoplastics. The occurrence of nanoplastics in the soil is expected to both directly and indirectly impact its properties and functioning. Nanoplastics may directly impact the physiology and development of living organisms, especially plants, e.g., by modifying their production yield. Nanoplastics can also indirectly modify the physicochemical properties of the soil and, as a result, favour the release of related contaminants (organic or inorganic) and have an impact on soil biota, and therefore have a negative effect on the functioning of rhizospheres. However all these results have to be taken carefully since performed with polymer nano-bead not representative of the nanoplastics observed in the environment. This review highlight thus the current knowledge on the interactions between plants, rhizosphere and nanoplastics, their consequences on plant physiology and development in order to identify gaps and propose scientific recommendations.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100473"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10142269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insight into the biocompatibility/toxicity of graphene oxides and their reduced forms on Chlamydomonas reinhardtii","authors":"Zuzana Bytešníková ,&nbsp;Martina Koláčková ,&nbsp;Markéta Dobešová ,&nbsp;Pavel Švec ,&nbsp;Andrea Ridošková ,&nbsp;Jana Pekárková ,&nbsp;Jan Přibyl ,&nbsp;Petr Cápal ,&nbsp;Dalibor Húska ,&nbsp;Vojtěch Adam ,&nbsp;Lukáš Richtera","doi":"10.1016/j.impact.2023.100468","DOIUrl":"10.1016/j.impact.2023.100468","url":null,"abstract":"<div><p>Graphene oxides (GOs) and their reduced forms are often discussed both positively and negatively due to the lack of information about their chemistry and structure. This study utilized GOs with two sheet sizes that were further reduced by two reducing agents (sodium borohydride and hydrazine) to obtain two different degrees of reduction. The synthesized nanomaterials were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), elemental analysis (EA), Fourier transform infrared (FTIR) spectroscopy, and Raman spectroscopy (RA) to understand their chemistry and structure. The second focus of our investigation included in vitro testing of the biocompatibility/toxicity of these materials on a model organism, the freshwater microalga <em>Chlamydomonas reinhardtii</em>. The effects were studied on the basis of biological endpoints complemented by biomass investigation (FTIR spectroscopy, EA, and atomic absorption spectrometry (AAS)). The results showed that the biocompatibility/toxicity of GOs is dependent on their chemistry and structure and that it is impossible to generalize the toxicity of graphene-based nanomaterials.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100468"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEG-GNPs aggravate MCD-induced steatohepatitic injury and liver fibrosis in mice through excessive lipid accumulation-mediated hepatic inflammatory damage","authors":"Hanqing Chen ,&nbsp;Shuang Zhou ,&nbsp;Wei Chen ,&nbsp;Meilin Zhu ,&nbsp;Hongyang Yu ,&nbsp;Lingna Zheng ,&nbsp;Bing Wang ,&nbsp;Meng Wang ,&nbsp;Weiyue Feng","doi":"10.1016/j.impact.2023.100469","DOIUrl":"10.1016/j.impact.2023.100469","url":null,"abstract":"<div><p>Rapid development of gold nanoparticles (GNPs) in delivering pharmaceutics and therapeutics approaches still linger the concerns of their toxic effects. Nonalcoholic steatohepatitis (NASH) is characterized by excessive lipid accumulation and overt hepatic inflammatory damage, and is the leading cause of chronic liver disease worldwide. This study aimed to assess the potential hepatic effects of GNPs on NASH phenotype and progression in mice. Mice were fed a MCD diet for 8 weeks to elicit NASH and then intravenously injected with PEG-GNPs at a single dose of 1, 5, and 25 mg/kg-bw. After 24 h and 1 week of administration, the levels of plasma ALT and AST, and the number of lipid droplets, the degree of lobular inflammation and the contents of triglycerides and cholesterols in the livers of the NASH mice significantly increased compared with the untreated NASH mice, indicating that the severity of MCD diet-induced NASH-like symptoms in mice increased after PEG-GNP administration. Moreover, the aggravated hepatic steatosis in a manner involving altered expression of the genes related to hepatic de novo lipogenesis, lipolysis, and fatty acid oxidation was observed after PEG-GNP administration. Additionally, the RNA levels of biomarkers of hepatic pro-inflammatory responses, endoplasmic reticulum stress, apoptosis, and autophagy in MCD-fed mice increased compared with the untreated NASH group. Moreover, PEG-GNP-treated NASH mice displayed an increase in MCD diet-induced hepatic fibrosis, revealed by massive deposition of collagen fiber in the liver and increased expression of fibrogenic genes. Collectively, these results suggest that hepatic GNP deposition after PEG-GNP administration increase the severity of MCD-induced NASH phenotype in mice, which is attributable to, in large part, increased steatohepatitic injury and liver fibrosis in mice.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"31 ","pages":"Article 100469"},"PeriodicalIF":4.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10139599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}