Molecular Biology of the Cell最新文献_第4页

Functional fluorescence labeling of actins in live-cell system using engineered split-GFP technology. 利用工程分裂- gfp技术对活细胞系统中肌动蛋白进行功能性荧光标记。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-13 DOI: 10.1091/mbc.E25-04-0171

Kaiming Xu, Dekuan Meng, Wei Li, Guangshuo Ou

{"title":"Functional fluorescence labeling of actins in live-cell system using engineered split-GFP technology.","authors":"Kaiming Xu, Dekuan Meng, Wei Li, Guangshuo Ou","doi":"10.1091/mbc.E25-04-0171","DOIUrl":"10.1091/mbc.E25-04-0171","url":null,"abstract":"Actin filaments play essential roles in various cellular processes, and understanding their dynamics is crucial for studying cellular behaviors and actin-related diseases. However, conventional methods for visualizing actins often perturb its functionality or lack sufficient resolution for real-time imaging. In this study, we developed a method for functional fluorescence labeling of actin isoforms using split-GFP (Green fluorescent protein) technology, specifically through insertion of a GFP11 tag into a flexible residue pair (T229/A230) of human β-actin (ACTB) or γ-actin (ACTG). This strategy (GFP11-i) was successfully applied to visualize actin dynamics in mammalian cell lines, including the effects of disease-related mutations (e.g., ACTB R196H and ACTG S155F). In addition, we demonstrated the labeling of actin filaments in Caenorhabditis elegans, further validating the cross-species applicability of this strategy. The GFP11-i methodology provides a versatile and powerful tool for investigating actin dynamics and cellular behaviors in both physiological and pathological contexts, facilitating the illustration of molecular mechanisms underlying actin-related diseases.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"mr9"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12444906/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Automated segmentation of soft X-ray tomography: Native cellular structure with submicron resolution at high-throughput for whole-cell quantitative imaging in yeast. 软x射线断层扫描的自动分割：酵母全细胞定量成像的亚微米分辨率高通量天然细胞结构。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-28 DOI: 10.1091/mbc.E24-10-0486

Jianhua Chen, Mary Mirvis, Axel Ekman, Bieke Vanslembrouck, Mark Le Gros, Carolyn Larabell, Wallace F Marshall

{"title":"Automated segmentation of soft X-ray tomography: Native cellular structure with submicron resolution at high-throughput for whole-cell quantitative imaging in yeast.","authors":"Jianhua Chen, Mary Mirvis, Axel Ekman, Bieke Vanslembrouck, Mark Le Gros, Carolyn Larabell, Wallace F Marshall","doi":"10.1091/mbc.E24-10-0486","DOIUrl":"10.1091/mbc.E24-10-0486","url":null,"abstract":"Soft X-ray tomography (SXT) is an invaluable tool for quantitatively analyzing cellular structures at suboptical isotropic resolution. However, it has traditionally depended on manual segmentation, limiting its scalability for large datasets. Here, we leverage a deep learning-based autosegmentation pipeline to segment and label cellular structures in hundreds of cells across three Saccharomyces cerevisiae strains. This task-based pipeline uses manual iterative refinement to improve segmentation accuracy for key structures, including the cell body, nucleus, vacuole, and lipid droplets, enabling high-throughput and precise phenotypic analysis. Using this approach, we quantitatively compared the three-dimensional (3D) whole-cell morphometric characteristics of wild-type, VPH1-GFP, and vac14 strains, uncovering detailed strain-specific cell and organelle size and shape variations. We show the utility of SXT data for precise 3D curvature analysis of entire organelles and cells and detection of fine morphological features using surface meshes. Our approach facilitates comparative analyses with high spatial precision and statistical throughput, uncovering subtle morphological features at the single-cell and population level. This workflow significantly enhances our ability to characterize cell anatomy and supports scalable studies on the mesoscale, with applications in investigating cellular architecture, organelle biology, and genetic research across diverse biological contexts.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar132"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polo-like kinase 1 maintains transcription and chromosomal accessibility during mitosis. polo样激酶1在有丝分裂期间维持转录和染色体可及性。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-13 DOI: 10.1091/mbc.E25-02-0053

Zhouyuan Shen, Kristin Adams, Ryan Moreno, Robert F Lera, Emily Kaufman, Jessica D Lang, Mark E Burkard

{"title":"Polo-like kinase 1 maintains transcription and chromosomal accessibility during mitosis.","authors":"Zhouyuan Shen, Kristin Adams, Ryan Moreno, Robert F Lera, Emily Kaufman, Jessica D Lang, Mark E Burkard","doi":"10.1091/mbc.E25-02-0053","DOIUrl":"10.1091/mbc.E25-02-0053","url":null,"abstract":"Transcription persists at low levels in mitotic cells and plays essential roles in mitotic fidelity and chromosomal dynamics. However, the detailed regulatory network of mitotic transcription remains largely unresolved. Here, we report the novel role of Polo-like kinase 1 (Plk1) in maintaining mitotic transcription. Using 5-ethynyl uridine (5-EU) labeling of nascent RNAs, we found that Plk1 inhibition leads to significant downregulation of nascent transcription in prometaphase cells. Chromatin-localized Plk1 activity is required for transcription regulation and mitotic fidelity. Plk1 sustains global chromosomal accessibility in mitosis, especially at promoter and transcription start site (promoter-TSS) regions, facilitating transcription factor binding and ensuring proper transcriptional activity. We identified SMC4, a common subunit of condensin I and II, as a potential Plk1 substrate. Plk1 activity is fundamental to these processes across nontransformed and transformed cell lines, underscoring its critical role in cell-cycle regulation. This study elucidates a novel regulatory mechanism of global mitotic transcription, advancing our understanding of cell-cycle control.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar128"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanics of Escherichia coli cell width homeostasis and bulge dynamics from MreB and septum inhibition. MreB和隔膜抑制对大肠杆菌细胞宽度稳态和膨胀动力学的影响。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-28 DOI: 10.1091/mbc.E24-12-0543

Tanvi Kale, Ryth Dasgupta, Mandar M Inamdar, Chaitanya A Athale

{"title":"Mechanics of Escherichia coli cell width homeostasis and bulge dynamics from MreB and septum inhibition.","authors":"Tanvi Kale, Ryth Dasgupta, Mandar M Inamdar, Chaitanya A Athale","doi":"10.1091/mbc.E24-12-0543","DOIUrl":"10.1091/mbc.E24-12-0543","url":null,"abstract":"Escherichia coli cell shape and size are governed by the mechanochemistry of the cellular components. Inhibiting either cell-wall synthesis proteins such as FtsI leads to cell elongation and bulging, while inhibiting MreB cytoskeletal polymerization results in a loss of rod-shape. Here, we quantify cell shape dynamics of E. coli combinatorially treated with the FtsI inhibitor cephalexin and MreB inhibitor A22 and fit a shell mechanics model to the length-width dynamics to infer the range of effective mechanical properties governing cell shape. The model based on the interplay of intracellular pressure and envelope mechanics, predicts E. coli cell width grows and saturates. Bulging observed in cells treated with both MreB and FtsI inhibitors, is predicted by the model to result from a lower effective bending rigidity and higher effective surface tension compared with untreated. We validate the specificity of the predicted internal pressure of ∼0.6 MPa driving bulging, when placing treated cells in a hyperosmotic environment of comparable pressure results in reversal of cell bulging. Simulations of cell width dynamics predicting threshold values of envelope bending rigidity and effective surface tension required to maintain cell shape compared with experiment validate the effective mechanical limits of cell shape maintenance.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar126"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Septin organization is regulated by the Gpa1 ubiquitination domain and endocytic machinery during the yeast pheromone response. 酵母信息素反应过程中，Septin组织受Gpa1泛素化结构域和内吞机制的调控。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-28 DOI: 10.1091/mbc.E25-05-0234

Cory P Johnson, Sudati Shrestha, Andrew Hart, Katherine F Jarvis, Loren Genrich, Sarah G Latario, Nicholas Leclerc, Tetiana Systuk, Matthew Scandura, Remi Geohegan, André Khalil, Joshua B Kelley

{"title":"Septin organization is regulated by the Gpa1 ubiquitination domain and endocytic machinery during the yeast pheromone response.","authors":"Cory P Johnson, Sudati Shrestha, Andrew Hart, Katherine F Jarvis, Loren Genrich, Sarah G Latario, Nicholas Leclerc, Tetiana Systuk, Matthew Scandura, Remi Geohegan, André Khalil, Joshua B Kelley","doi":"10.1091/mbc.E25-05-0234","DOIUrl":"10.1091/mbc.E25-05-0234","url":null,"abstract":"Saccharomyces cerevisiae detect and respond to mating pheromone using a G-protein-coupled receptor signaling pathway (GPCR) to initiate polarized growth toward mating partners. Septins form structures at the base of the mating projection to control morphogenesis in a manner that is dependent upon desensitization of the large G-protein Gpa1. We sought to identify the pathway through which Gpa1 regulates septin organization using gene deletions in the presence of a hyperactive Gpa1 mutant, live cell imaging, and computational approaches. We found that the Cdc42 effector Gic1, the Cdc42 GAP Bem3, and the endocytic proteins Ent1, Ent2, and End3 are all involved in Gpa1 organization of septins. Mathematical modeling indicates that changes in the location of endocytic events could be responsible for the observed septin localization in the varied genetic backgrounds. Both the GPCR Ste2 and Gpa1 undergo endocytosis. We found that while the Ste2 c-terminus impacts septin organization in the presence of WT Gpa1, hyperactive Gpa1 drives septin organization through its ubiquitin domain that is required for its endocytosis. These data support a model where cargo recruitment of epsins (Ent1/2) and Cdc42 GAPs couples endocytosis with septin organization.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar125"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Gα-MAPK interaction in mating of Saccharomyces cerevisiae. Gα-MAPK互作在酿酒酵母交配中的作用

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-20 DOI: 10.1091/mbc.E23-09-0362

Corrina G Robertson, Erin R Curtis, Olivia Gorman, Lyana Matonti, Daniel J Lew

{"title":"Role of Gα-MAPK interaction in mating of Saccharomyces cerevisiae.","authors":"Corrina G Robertson, Erin R Curtis, Olivia Gorman, Lyana Matonti, Daniel J Lew","doi":"10.1091/mbc.E23-09-0362","DOIUrl":"10.1091/mbc.E23-09-0362","url":null,"abstract":"Guidance of cell growth or movement in response to chemical cues in the environment is critical for many cell behaviors. Budding yeast orientation of polarized growth in response to gradients of mating pheromones provides a tractable model to address how cells accurately assess small spatial differences in chemical concentrations. Pheromones bind to receptors that act through heterotrimeric G proteins to promote activation of the MAPK Fus3. Active Fus3 binds to Gα, which is thought to enhance local phosphorylation of relevant MAPK substrates to promote orientation of polarity toward high-pheromone regions. Polarity is oriented by a pathway in which Gβγ binds the scaffold protein Far1 to activate the conserved polarity regulator Cdc42, which activates the formin Bni1 to orient actin and hence growth. Gβγ, Far1, and Bni1 are all MAPK substrates whose phosphorylation could improve orientation toward high-pheromone regions. Here, we show that the Gα-MAPK interaction can enhance the efficiency of polarity-site alignment between mating partners, particularly under conditions with high Fus3 activity. Surprisingly, however, we find no evidence that phosphorylation of Gβγ, Far1, or Bni1 contribute to the benefit conferred by Gα-MAPK interaction. The precise role of this interaction remains mysterious.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"br24"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trimerization domain-interfering peptide inhibits EML4-ALK condensate formation, fusion-dependent signaling, and cell growth. 三聚体结构域干扰肽抑制EML4-ALK凝聚形成，融合依赖的信号传导和细胞生长。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-28 DOI: 10.1091/mbc.E25-03-0113

Kyle Scheller, Xin Zhou, Kun Li, Lan Jiang, Rodrigo Ochoa, Pilar Cossio, Lizi Wu, Juan Guan

{"title":"Trimerization domain-interfering peptide inhibits EML4-ALK condensate formation, fusion-dependent signaling, and cell growth.","authors":"Kyle Scheller, Xin Zhou, Kun Li, Lan Jiang, Rodrigo Ochoa, Pilar Cossio, Lizi Wu, Juan Guan","doi":"10.1091/mbc.E25-03-0113","DOIUrl":"10.1091/mbc.E25-03-0113","url":null,"abstract":"Biomolecular condensates are micrometer-scale subcellular structures assembled through protein phase separation in living cells. Recent research shows that they are critical to normal biological processes and their misregulation may contribute to disease. A prominent example is the cancer-causing EML4-ALK fusion protein, which spontaneously forms biomolecular condensates that significantly enhance receptor tyrosine kinase (RTK) signaling within the condensate microenvironment. In this work, we show that a trimerization domain (TD) in EML4-ALK is necessary for condensate formation. By designing a peptide targeting the TD, we disrupted EML4-ALK self-assembly, leading to the dissolution of pre-existing EML4-ALK condensates in patient lung tumor-derived cells. Notably, this disruption significantly reduced EML4-ALK-dependent signaling and cell proliferation. Our findings demonstrate that interfering with a specific protein-protein interaction can disrupt oncogenic biomolecular condensates and attenuate their associated signaling. These results highlight the potential of targeting condensate assembly as a strategy to modulate oncogenic signaling.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"br25"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRC1 resists microtubule sliding in two distinct resistive modes due to variations in the separation between overlapping microtubules. 由于重叠微管之间的分离变化，PRC1以两种不同的电阻模式抵抗微管滑动。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-07-02 DOI: 10.1091/mbc.E25-06-0288

Daniel Steckhahn, Shane A Fiorenza, Ellinor Tai, Scott Forth, Peter R Kramer, Meredith Betterton

{"title":"PRC1 resists microtubule sliding in two distinct resistive modes due to variations in the separation between overlapping microtubules.","authors":"Daniel Steckhahn, Shane A Fiorenza, Ellinor Tai, Scott Forth, Peter R Kramer, Meredith Betterton","doi":"10.1091/mbc.E25-06-0288","DOIUrl":"10.1091/mbc.E25-06-0288","url":null,"abstract":"Cross-linked cytoskeletal filament networks provide cells with a mechanism to regulate cellular mechanics and force transmission. An example in the microtubule cytoskeleton is mitotic spindle elongation. The three-dimensional geometry of these networks, including the overlap length and lateral microtubule spacing, likely controls how forces can be regulated, but how these parameters evolve during filament sliding is unknown. Recent evidence suggests that the cross-linker PRC1 can resist microtubule sliding by two distinct modes: a braking mode and a less resistive coasting mode. To explore how molecular-scale mechanisms influence network geometry in this system, we developed a computational model of sliding microtubule pairs cross-linked by PRC1 that reproduces the experimentally observed braking and coasting modes. Surprisingly, we found that the braking mode was associated with a substantially smaller lateral separation between the cross-linked microtubules than the coasting mode. This closer separation aligns the PRC1-mediated forces against sliding, increasing the resistive PRC1 force and dramatically reducing sliding speed. The model also finds an emergent similar average sliding speed due to PRC1 resistance, because higher initial sliding speed favors the transition to braking. Together, our results highlight the importance of the three-dimensional geometric relationships between cross-linkers and microtubules.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar115"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alterations in lipid saturation trigger remodeling of the outer mitochondrial membrane. 脂质饱和度的改变引发线粒体外膜的重塑。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-07-30 DOI: 10.1091/mbc.E25-01-0033

Sara Wong, Katherine R Bertram, Sai Sangeetha Balasubramaniam, Nidhi Raghuram, Thomas Knight, J Alan Maschek, James E Cox, Adam L Hughes

{"title":"Alterations in lipid saturation trigger remodeling of the outer mitochondrial membrane.","authors":"Sara Wong, Katherine R Bertram, Sai Sangeetha Balasubramaniam, Nidhi Raghuram, Thomas Knight, J Alan Maschek, James E Cox, Adam L Hughes","doi":"10.1091/mbc.E25-01-0033","DOIUrl":"10.1091/mbc.E25-01-0033","url":null,"abstract":"Lipid saturation is a key determinant of membrane function and organelle health, with changes in saturation triggering adaptive quality control mechanisms to maintain membrane integrity. Among cellular membranes, the outer mitochondrial membrane (OMM) is an important interface for many cellular functions, but how lipid saturation impacts OMM function remains unclear. Here, we show that increased intracellular unsaturated fatty acids (UFA) remodel the OMM by promoting the formation of multilamellar mitochondrial-derived compartments (MDC), which sequester proteins and lipids from the OMM. These effects depend on the incorporation of UFAs into membrane phospholipids, suggesting that changes in membrane bilayer composition mediate this process. Furthermore, elevated UFAs impair the assembly of the OMM protein translocase (TOM, translocase of the outer membrane) complex, with unassembled TOM components captured into MDCs. Collectively, these findings suggest that alterations in phospholipid saturation may destabilize OMM protein complexes and trigger an adaptive response to sequester excess membrane proteins through MDC formation.","PeriodicalId":18735,"journal":{"name":"Molecular Biology of the Cell","volume":" ","pages":"ar129"},"PeriodicalIF":2.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12483321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144753837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Please inhibit responsibly: Natural and synthetic actin toxins as useful tools in cell biology. 请负责任地抑制：作为细胞生物学有用工具的天然和合成肌动蛋白毒素。

IF 2.7 3区生物学

Molecular Biology of the Cell Pub Date : 2025-10-01 Epub Date: 2025-08-13 DOI: 10.1091/mbc.E25-05-0264

Katrina B Velle, Masayuki Onishi

引用次数: 0