Molecular biology and evolution最新文献_第9页

Selection Maintains Photosynthesis in a Symbiotic Cyanobacterium Despite Redundancy With its Fern Host. 选择维持光合作用在共生蓝藻尽管冗余与它的蕨类宿主。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf181

Liam Friar, Kyle Keepers, Arkadiy I Garber, John P McCutcheon, Boswell Wing, Nolan C Kane

{"title":"Selection Maintains Photosynthesis in a Symbiotic Cyanobacterium Despite Redundancy With its Fern Host.","authors":"Liam Friar, Kyle Keepers, Arkadiy I Garber, John P McCutcheon, Boswell Wing, Nolan C Kane","doi":"10.1093/molbev/msaf181","DOIUrl":"10.1093/molbev/msaf181","url":null,"abstract":"Vertically inherited symbionts experience different physical, chemical, and population genetic environments than free-living organisms. As a result, they can experience long-term reductions in effective population size (Ne) and weaker purifying selection on genes that are less important in the host-associated environment. Over time, these forces result in gene loss. A comparative genomic approach using independently evolved symbiotic bacteria and free-living relatives can reveal which genes are important in the symbiotic state. We apply this approach to understand why some diazotrophic cyanobacteria evolving as vertically inherited symbionts of photosynthetic eukaryotic hosts have lost their ancestral capacity for photosynthesis while others have retained that capacity. We look specifically at Trichormus azollae, a diazotrophic cyanobacterium that remains photosynthetic after 50 to 90 Ma as a vertically inherited symbiont of Azolla ferns. We show that gene loss is ongoing, with different genes lost across the eight T. azollae strains examined. We apply molecular evolutionary models to genomes of T. azollae and free-living relatives, finding genome-wide signatures of drift in T. azollae consistent with long-term reductions in Ne. Ribosomal proteins and proteins from the energy-capturing photosynthetic light reactions are under stronger purifying selection than genes from other pathways, including nitrogen fixation and photosynthetic carbon fixation. Strong purifying selection is expected for the ribosome given its extraordinary levels of conservation, even in ancient vertically inherited symbionts. That genes in the light reactions are under strong purifying selection and never lost in any strain suggests that energy capture, likely required for energy-intensive nitrogen fixation, remains important to this symbiont.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12362246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repressive Cytosine Methylation is a Marker of Viral Gene Transfer Across Divergent Eukaryotes. 抑制胞嘧啶甲基化是病毒基因在不同真核生物间转移的标志。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf176

Luke A Sarre, Giselle Azucena Gastellou Peralta, Pedro Romero Charria, Vladimir Ovchinnikov, Alex de Mendoza

{"title":"Repressive Cytosine Methylation is a Marker of Viral Gene Transfer Across Divergent Eukaryotes.","authors":"Luke A Sarre, Giselle Azucena Gastellou Peralta, Pedro Romero Charria, Vladimir Ovchinnikov, Alex de Mendoza","doi":"10.1093/molbev/msaf176","DOIUrl":"10.1093/molbev/msaf176","url":null,"abstract":"Cytosine DNA methylation patterns vary widely across eukaryotes, with its ancestral roles being understood to have included both transposable element (TE) silencing and host gene regulation. To further explore these claims, in this study, we reevaluate the evolutionary origins of DNA methyltransferases and characterize the roles of cytosine methylation on underexplored lineages, including the amoebozoan Acanthamoeba castellanii, the glaucophyte Cyanophora paradoxa, and the heterolobosean Naegleria gruberi. Our analysis of DNA methyltransferase evolution reveals a rich ancestral eukaryotic repertoire, with several eukaryotic lineages likely subsequently acquiring enzymes through lateral gene transfer (LGT). In the three species examined, DNA methylation is enriched on young TEs and silenced genes, suggesting an ancestral repressive function, without the transcription-linked gene body methylation of plants and animals. Consistent with this link with silencing, methylated genomic regions co-localize with heterochromatin marks, including H3K9me3 and H3K27me3. Notably, the closest homologs of many of the silenced, methylated genes in diverse eukaryotes belong to viruses, including giant viruses. Given the widespread occurrence of this pattern across diverse eukaryotic groups, we propose that cytosine methylation was a silencing mechanism originally acquired from bacterial donors, which was used to mitigate the expression of both transposable and viral elements, and that this function may persist in creating a permissive atmosphere for LGT in diverse eukaryotic lineages. These findings further highlight the importance of epigenetic information to annotate eukaryotic genomes, as it helps delimit potentially adaptive LGTs from silenced parasitic elements.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12344493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copy Number Variation Shapes Structural Genomic Diversity Associated With Ecological Adaptation in the Wild Tomato Solanum chilense. 拷贝数变异决定了野生番茄茄（Solanum chilense）与生态适应相关的结构基因组多样性。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf191

Kai Wei, Remco Stam, Aurélien Tellier, Gustavo A Silva-Arias

{"title":"Copy Number Variation Shapes Structural Genomic Diversity Associated With Ecological Adaptation in the Wild Tomato Solanum chilense.","authors":"Kai Wei, Remco Stam, Aurélien Tellier, Gustavo A Silva-Arias","doi":"10.1093/molbev/msaf191","DOIUrl":"10.1093/molbev/msaf191","url":null,"abstract":"Copy Number Variation (CNV) is a prevalent type of variation affecting large genomic regions which contributes to both genetic diversity and ecological adaptation in plants. The target genes involved in adaptation through CNV in tomato and its wild relatives remain unexplored at the population level. Therefore, we characterized the CNV landscape of Solanum chilense, a wild tomato species adapted to dry habitats, using whole-genome short-read data of 35 individuals from 7 populations. We identified 212,207 CNVs, including 160,926 deletions and 51,281 duplications. We found a higher number of CNVs in diverging populations occupying stressful habitats. CNVs and single-nucleotide polymorphism analyses concordantly revealed the known species' population structure, underscoring the impact of historical demographic and recent colonization events shaping genome-wide CNVs. Furthermore, we identified 3,539 candidate genes with highly divergent CNV profiles across populations. Interestingly, these genes are functionally associated with response to abiotic stress and linked to multiple pathways of flowering time regulation. Gene CNVs in S. chilense exhibit 2 evolutionary trends: gene loss in ancestral lineages distributed in central and southern coast populations and gene gain in the most recent diverged lineage from the southern highland region. Environmental association of the CNVs ultimately linked the dynamics of gene copy number to 6 climatic variables. It suggests that natural selection has likely shaped CNV patterns in stress-response genes, promoting the colonization of contrasting habitats. Our findings provide insights into the role of CNV underlying adaptation during recent range expansion.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144784833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Patterns of Loss of Distyly and Polyploidization in Primroses. 报春花颖花丧失和多倍体化的基因组模式。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf162

Emiliano Mora-Carrera, Narjes Yousefi, Giacomo Potente, Rebecca Lynn Stubbs, Barbara Keller, Étienne Léveillé-Bourret, Stefan Grob, Ferhat Celep, Giorgi Tedoradze, Elena Conti

{"title":"Genomic Patterns of Loss of Distyly and Polyploidization in Primroses.","authors":"Emiliano Mora-Carrera, Narjes Yousefi, Giacomo Potente, Rebecca Lynn Stubbs, Barbara Keller, Étienne Léveillé-Bourret, Stefan Grob, Ferhat Celep, Giorgi Tedoradze, Elena Conti","doi":"10.1093/molbev/msaf162","DOIUrl":"10.1093/molbev/msaf162","url":null,"abstract":"Transitions from outcrossing to selfing and from diploidy to polyploidy often co-occur in plants, likely because the ability to produce selfed seed increases the likelihood of newly formed polyploids to become established. An ideal system to study these transitions is Primula, where the shift from diploid, outcrossing progenitors to polyploid, selfing descendants co-occurred repeatedly and the genetic basis of the mating-system shift is known. In Primula, outcrossing is enforced in distylous, typically diploid species characterized by florally heteromorphic, self-incompatible individuals, whereas selfing is enabled in homostylous, typically polyploid species, characterized by florally homomorphic populations of self-compatible plants. Distyly is controlled by the S-locus supergene. Small loss-of-function mutations in the S-locus CYPT gene, which controls style length and female self-incompatibility, are associated with loss of heterostyly in diploid, ancestrally heterostylous Primula species. However, CYPT and the S-locus have never been investigated in interspecific shifts from distylous, diploid species to homostylous, polyploid species. By analyzing the first assembled genome of a homostylous, polyploid species (Primula grandis) in a comparative framework, we discovered two, nearly identical S-locus alleles in the same subgenome, consistent with the hypothesis that the species originated from a cross between a homostylous, diploid pollen donor and a long-styled, diploid pollen recipient. Conformant to theoretical predictions, the macroevolutionary loss of distyly coincided with considerable degeneration of CYPT, including multiple mutations and exon loss, while other S-locus genes remained largely unaffected. This study advances knowledge on the macroevolutionary dynamics of supergenes and genomes in shifts between breeding systems and ploidy levels.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12312358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sex-Biased Gene Expression Under Sexually Antagonistic and Sex-Limited Selection. 性别对抗和性别限制选择下的性别偏倚基因表达。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf178

R Axel W Wiberg, Martyna K Zwoinska, Philipp Kaufmann, James M Howie, Elina Immonen

{"title":"Sex-Biased Gene Expression Under Sexually Antagonistic and Sex-Limited Selection.","authors":"R Axel W Wiberg, Martyna K Zwoinska, Philipp Kaufmann, James M Howie, Elina Immonen","doi":"10.1093/molbev/msaf178","DOIUrl":"10.1093/molbev/msaf178","url":null,"abstract":"Sex differences in gene expression are ubiquitous, evolve quickly, and are expected to underlie phenotypic sexual dimorphism (SD). Despite long-standing interest, the impact of sex-specific selection on the transcriptome remains poorly understood. Here, we test fundamental questions on the role of constraints on gene expression evolution arising from the mode of selection and genetic architecture. We also test the relationship between sex-biased expression and evolved SD. We assess these using body size selection lines in the seed beetle, Callosobruchus maculatus, that have evolved variation in SD in response to either sex-limited (SL) or sexually antagonistic (SA). We find that sex differences in the phenotypic responses and expression changes are generally well aligned. SL selection, despite a phenotypic response similar to SA selection in males, but not in females, resulted in a more extensive expression differentiation and increase of sex-biased expression than SA selection. These patterns show that SA selection imposes a transcriptomic constraint and is not required for sex-bias to evolve. Sex-biased transcripts show lower cross-sex correlations in expression changes than unbiased transcripts, suggesting greater sex differences in their underlying genetic architecture. Although male-biased transcripts are disproportionately affected when selection targeted males, we find no support for a transcriptome-wide association between sex-bias and SD. In the light of these unique experimental insights into how sex-specific selection on size changes adult transcription, our findings have important implications for inferring selection history and mode from patterns of sex-biased gene expression in natural populations.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12371189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144743142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyploid Formation Through Disrupting Mitotic Sister Chromatid Separation of Spermatogonia based on espl1 Heterozygous Knockout in Fish. 基于espl1杂合基因敲除破坏精原细胞有丝分裂姐妹染色单体分离形成多倍体。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf195

Yunbang Zhang, Yihui Mei, Takafumi Fujimoto, Jian Gao, Yuwei Huang, Yuxuan Zheng, Rongyun Li, Yankun Guo, Nan Zhang, Yuxin Jiang, Lanhui Ouyang, Hanjun Jiang, Katsutoshi Arai, Xiaojuan Cao

{"title":"Polyploid Formation Through Disrupting Mitotic Sister Chromatid Separation of Spermatogonia based on espl1 Heterozygous Knockout in Fish.","authors":"Yunbang Zhang, Yihui Mei, Takafumi Fujimoto, Jian Gao, Yuwei Huang, Yuxuan Zheng, Rongyun Li, Yankun Guo, Nan Zhang, Yuxin Jiang, Lanhui Ouyang, Hanjun Jiang, Katsutoshi Arai, Xiaojuan Cao","doi":"10.1093/molbev/msaf195","DOIUrl":"10.1093/molbev/msaf195","url":null,"abstract":"Polyploidy is a major driver of speciation and evolutionary changes in plants and animals. Production of unreduced gametes is considered as a main pathway for polyploid formation. However, the precise molecular mechanisms underlying unreduced gamete production, particularly those arising from mitotic defects of spermatogonia (SG)/oogonia, remain poorly understood. Here, a heterozygous espl1 (extra spindle pole bodies like 1) knockout line was generated in diploid loach (Misgurnus anguillicaudatus) by CRISPR/Cas9 technology. Interestingly, we found about 90% diploids and 10% triploids in the progeny of espl1+/- males and wild-type (WT) diploid females. espl1+/- male could produce 1n sperms, along with a certain volume of unreduced sperms (2n). All offsprings of espl1+/-♀ and espl1+/+♂ were diploid, indicating that espl1+/- female produced normal ploidy eggs. Heterozygous espl1 deficiency impaired mitotic sister chromatid separation of some SG, resulting in their chromosome number doubling, thus causing the unreduced sperm production. The triploid loach (espl1+/+/-) from espl1+/+♀ mating with espl1+/-♂ could produce triploid sperms, which gave tetraploid heterozygotes by fertilization with haploid eggs. Resultant tetraploids yielded all-triploid progeny, when mated with WT diploid males. This study was extended to model fish zebrafish, where heterozygous espl1 knockout zebrafish produced about 5% unreduced diploid sperms. Here, we showed that the heterozygous loss of espl1 was enough to induce spermatogonial mitotic sister chromatid separation defects, causing the production of unreduced sperms. Notably, our results provide new strategies for the aquaculture-oriented polyploid breeding.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12391874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144835722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Importance of Regulatory Network Structure for Complex Trait Heritability and Evolution. 调控网络结构对复杂性状遗传和进化的重要性。

IF 5.3 1区生物学

Molecular biology and evolution Pub Date : 2025-07-30 DOI: 10.1093/molbev/msaf174

Katherine L Stone, John Platig, John Quackenbush, Maud Fagny

{"title":"The Importance of Regulatory Network Structure for Complex Trait Heritability and Evolution.","authors":"Katherine L Stone, John Platig, John Quackenbush, Maud Fagny","doi":"10.1093/molbev/msaf174","DOIUrl":"10.1093/molbev/msaf174","url":null,"abstract":"Complex traits are determined by many loci-mostly regulatory elements-that, through combinatorial interactions, can affect multiple traits. Such high levels of epistasis and pleiotropy have been proposed in the omnigenic model and may explain why such a large part of complex trait heritability is usually missed by genome-wide association studies, while raising questions about the possibility for such traits to evolve in response to environmental constraints. To explore the molecular bases of complex traits and understand how they can adapt, we systematically analyzed the distribution of SNP heritability for 11 traits across 29 tissue-specific expression quantitative trait locus networks. We find that heritability is clustered in a small number of tissue-specific, functionally relevant SNP-gene modules and that the greatest heritability occurs in local \"hubs\" that are both the cornerstone of the network's modules and tissue-specific regulatory elements. The network structure could thus both amplify the genotype-phenotype connection and buffer the deleterious effect of the genetic variations on other traits. We confirm that this structure has allowed complex traits to evolve in response to environmental constraints, with the local \"hubs\" being the preferential targets of past and ongoing directional selection. Together, these results provide a conceptual framework for understanding complex trait architecture and evolution.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":"42 8","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12362071/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Divergence of Leptin Receptor and Interleukin-6 Receptor Subunit b in Early Vertebrate Evolution and Physiological Insights from the Sea Lamprey. 瘦素受体和白细胞介素-6受体亚基b在早期脊椎动物进化中的分化及来自海七鳃鳗的生理学启示。

IF 11 1区生物学

Molecular biology and evolution Pub Date : 2025-07-01 DOI: 10.1093/molbev/msaf157

Ningping Gong, André Barany, Jessica L Norstog, Dan Larhammar, Björn Thrandur Björnsson, Amy M Regish, Stephen D McCormick, Mark A Sheridan

{"title":"Divergence of Leptin Receptor and Interleukin-6 Receptor Subunit b in Early Vertebrate Evolution and Physiological Insights from the Sea Lamprey.","authors":"Ningping Gong, André Barany, Jessica L Norstog, Dan Larhammar, Björn Thrandur Björnsson, Amy M Regish, Stephen D McCormick, Mark A Sheridan","doi":"10.1093/molbev/msaf157","DOIUrl":"10.1093/molbev/msaf157","url":null,"abstract":"Current knowledge of class-I cytokine receptors comes primarily from studies in jawed vertebrates (gnathostomes), and their origin and evolution remain unresolved. In this study, we identified a leptin receptor-like sequence (LepRL) and three interleukin-6 receptor subunit b-like sequences (IL6RBL) from a jawless vertebrate (cyclostome), the sea lamprey (Petromyzon marinus). Based on structural, phylogenetic, and syntenic analyses, we deduced that these lamprey receptors are likely distinct ohnologs to gnathostome LepR and IL6RB-related receptors, respectively, that arose in the two rounds of vertebrate whole-genome duplication (1R and 2R). Notably, lamprey LepRL likely originated from a different 1R progenitor than the one giving rise to gnathostome LepR during cyclostome hexaploidization. Differential patterns in mRNA expression of LepRL and IL6RBLs were observed among adult tissues, during larval metamorphosis, and in response to juvenile feeding. Feeding stimulated hepatic expression of LepRL and IL6RBL (namely, IL6RBL1) mRNAs in correlation with upregulation of insulin-like growth factor mRNA, whereas brain LepRL and IL6RBL1 mRNA expression was correlated positively with neuropeptide Y but inversely with intestinal content in fed juveniles. Notably, these observations along with immunolocalization of LepRL in the hypothalamus suggest a role of leptin signaling in regulating energy balance that is conserved among vertebrates. Additionally, seawater exposure stimulated branchial LepRL expression coincident with increased expression of ion transporters in ionocytes, indicating a role of leptin signaling in osmoregulation. These findings provide new insight into the early evolution of class-I cytokine receptors and reveal diverse functions of the leptin signaling system in jawless vertebrate.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":11.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Divergence in Response to Sex-biased Selection. 性别偏向选择反应中的表达差异。

IF 11 1区生物学

Molecular biology and evolution Pub Date : 2025-07-01 DOI: 10.1093/molbev/msaf099

Karl Grieshop, Michelle J Liu, Ryan S Frost, Matthew P Lindsay, Malak Bayoumi, Martin I Brengdahl, Ruxandra I Molnar, Aneil F Agrawal

{"title":"Expression Divergence in Response to Sex-biased Selection.","authors":"Karl Grieshop, Michelle J Liu, Ryan S Frost, Matthew P Lindsay, Malak Bayoumi, Martin I Brengdahl, Ruxandra I Molnar, Aneil F Agrawal","doi":"10.1093/molbev/msaf099","DOIUrl":"10.1093/molbev/msaf099","url":null,"abstract":"It remains debated whether greater degrees of sexual dimorphism would evolve if not for intersexual genetic constraints. Here, we used experimental evolution to partially break the intersexual genetic constraint in Drosophila melanogaster to investigate the effects of a shared gene pool on the evolution of sexual dimorphism in gene expression. In six replicate populations of 1,000 flies, a dominant marker (DsRed) was used to force a \"Red\" pool of genetically variable Chromosome 2 copies through exclusive father-to-son inheritance, while a complimentary pool of \"NonRed\" chromosomes was inherited primarily from mothers to daughters. After 100 generations, we demonstrated the effect of Red male-limited chromosomes in increasing male mating success. Differentially expressed genes between flies with and without Red chromosomes had on average higher intersexual genetic correlations (rMF), as expected if such correlations represent a constraint to sex-specific adaptation under normal inheritance. If conflict hinders the evolution of further dimorphism, the transcriptomes of male-selected Red chromosomes were predicted to evolve to be \"masculinized\" relative to female-selected NonRed chromosomes. Consistent with this, splicing patterns in Red males (but not Red females) were masculinized relative to NonRed males. Contrastingly, gene expression levels were largely feminized in Red flies of both sexes compared with NonRed. We discuss alternative forms of intralocus sexual conflict that may explain these patterns.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":"42 7","pages":""},"PeriodicalIF":11.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Heterogametic Transition in European Bufo Toads Switches the Sex Linkage of Key Vertebrate Sex Determination Genes and Associates with a Large Sex Chromosome Effect. 欧洲蟾蜍的异配子转变改变了关键脊椎动物性别决定基因的性别连锁，并与巨大的性染色体效应有关。

IF 11 1区生物学

Molecular biology and evolution Pub Date : 2025-07-01 DOI: 10.1093/molbev/msaf142

Christophe Dufresnes, Pierre-André Crochet, Beata Rozenblut-Kościsty, Spartak N Litvinchuk, Nicolas Rodrigues, Nicolas Perrin, Daniel L Jeffries

{"title":"The Heterogametic Transition in European Bufo Toads Switches the Sex Linkage of Key Vertebrate Sex Determination Genes and Associates with a Large Sex Chromosome Effect.","authors":"Christophe Dufresnes, Pierre-André Crochet, Beata Rozenblut-Kościsty, Spartak N Litvinchuk, Nicolas Rodrigues, Nicolas Perrin, Daniel L Jeffries","doi":"10.1093/molbev/msaf142","DOIUrl":"10.1093/molbev/msaf142","url":null,"abstract":"Characterizing the diversity and lability of the amphibian sex chromosomes holds key to understand what drives sex chromosome turnovers and assess the role of sex-linked genes in reproductive isolation and speciation. Here, we show that the heterogametic transition previously reported between the hybridizing toads Bufo bufo (ZW) and Bufo spinosus (XY) is nonhomologous, potentially implicates key genes of the vertebrate sex determination cascade (SOX9, DMRT1, and AMH), and is characterized by a much shorter ZW than XY segment. Integrating this information with published hybrid zone data suggests that both sex chromosomes resist interspecific introgression more than autosomes. These observations substantiate that sex chromosome turnovers preferentially involve chromosomes that host conserved sex-determining genes, imply heterochiasmy as a key factor of sex chromosome differentiation, and are consistent with a large sex chromosome effect, an empirical rule of speciation that is not expected with homomorphic sex chromosomes.","PeriodicalId":18730,"journal":{"name":"Molecular biology and evolution","volume":" ","pages":""},"PeriodicalIF":11.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12214457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0