Molecular and Cellular Biochemistry最新文献_第10页

The interplay between oxidative stress, zinc, and metabolic dysfunction in polycystic ovarian syndrome 多囊卵巢综合征中氧化应激、锌和代谢功能障碍之间的相互作用

IF 4.3 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-12 DOI: 10.1007/s11010-024-05113-x

Olivia G. Camp, Daniel N. Moussa, Richard Hsu, Awoniyi O. Awonuga, Husam M. Abu-Soud

引用次数: 0

Transcription factor Yin Yang 1 enhances epithelial-mesenchymal transition, migration, and stemness of non-small cell lung cancer cells by targeting sonic hedgehog 转录因子阴阳1通过靶向音速刺猬增强非小细胞肺癌细胞的上皮-间质转化、迁移和干性

IF 4.3 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-11 DOI: 10.1007/s11010-024-05104-y

Tonghai Huang, Kangqi Ren, Xiean Ling, Zeyao Li, Lin Chen

{"title":"Transcription factor Yin Yang 1 enhances epithelial-mesenchymal transition, migration, and stemness of non-small cell lung cancer cells by targeting sonic hedgehog","authors":"Tonghai Huang, Kangqi Ren, Xiean Ling, Zeyao Li, Lin Chen","doi":"10.1007/s11010-024-05104-y","DOIUrl":"https://doi.org/10.1007/s11010-024-05104-y","url":null,"abstract":"Non-small cell lung cancer (NSCLC) is a frequent type of lung cancer. Transcription factor Yin Yang 1 (YY1), an endogenous transcription factor containing zinc finger structure, can accelerate NSCLC progression. However, the impact of YY1 on the stemness of NSCLC cells and the mechanism of promoting NSCLC cell progression is unclear. YY1 and Sonic hedgehog (Shh) expressions were monitored by RT-qPCR, western blot, and immunohistochemistry. Overall survival was tested through Kaplan–Meier analysis. The interaction between YY1 and Shh was confirmed. Then, cell migration, stemness, and epithelial-mesenchymal transition (EMT) were assessed with functional experiments in vitro and in vivo. YY1 and Shh were highly expressed in NSCLC tissues and positively correlated with the poor OS of NSCLC patients. Functional experiments denoted that YY1 or Shh overexpression could accelerate EMT, migration, and stemness of NSCLC cells, and YY1 or Shh knockdown played the opposite role to its overexpression. Mechanism analysis disclosed that Shh, as a target gene of YY1, was positively related to YY1. The rescued experiment manifested that Shh silencing could reverse the induction effect of YY1 overexpression on EMT, migration, and stemness of NSCLC cells. In vivo experiments also confirmed that YY1 could accelerate tumor growth and EMT and weaken apoptosis. YY1 promotes NSCLC EMT, migration, and stemness by Shh, which might be novel diagnostic markers and therapeutic targets for NSCLC therapy.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pericardium decellularization in a one-day, two-step protocol 一天两步心包脱细胞方案

IF 4.3 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-10 DOI: 10.1007/s11010-024-05086-x

P. López-Chicón, J. I. Rodríguez Martínez, C. Castells-Sala, L. Lopez-Puerto, L. Ruiz-Ponsell, O. Fariñas, A. Vilarrodona

{"title":"Pericardium decellularization in a one-day, two-step protocol","authors":"P. López-Chicón, J. I. Rodríguez Martínez, C. Castells-Sala, L. Lopez-Puerto, L. Ruiz-Ponsell, O. Fariñas, A. Vilarrodona","doi":"10.1007/s11010-024-05086-x","DOIUrl":"https://doi.org/10.1007/s11010-024-05086-x","url":null,"abstract":"Scaffolds used in tissue engineering can be obtained from synthetic or natural materials, always focusing the effort on mimicking the extracellular matrix of human native tissue. In this study, a decellularization process is used to obtain an acellular, biocompatible non-cytotoxic human pericardium graft as a bio-substitute. An enzymatic and hypertonic method was used to decellularize the pericardium. Histological analyses were performed to determine the absence of cells and ensure the integrity of the extracellular matrix (ECM). In order to measure the effect of the decellularization process on the tissue’s biological and mechanical properties, residual genetic content and ECM biomolecules (collagen, elastin, and glycosaminoglycan) were quantified and the tissue’s tensile strength was tested. Preservation of the biomolecules, a residual genetic content below 50 ng/mg dry tissue, and maintenance of the histological structure provided evidence for the efficacy of the decellularization process, while preserving the ECM. Moreover, the acellular tissue retains its mechanical properties, as shown by the biomechanical tests. Our group has shown that the acellular pericardial matrix obtained through the super-fast decellularization protocol developed recently retains the desired biomechanical and structural properties, suggesting that it is suitable for a broad range of clinical indications.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNAs as promising drug delivery target to ameliorate chronic obstructive pulmonary disease using nano-carriers: a comprehensive review 利用纳米载体改善慢性阻塞性肺病，微RNA是前景广阔的给药靶标：综述

IF 4.3 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-10 DOI: 10.1007/s11010-024-05110-0

Vamika Khanna, Kavita Singh

{"title":"MicroRNAs as promising drug delivery target to ameliorate chronic obstructive pulmonary disease using nano-carriers: a comprehensive review","authors":"Vamika Khanna, Kavita Singh","doi":"10.1007/s11010-024-05110-0","DOIUrl":"https://doi.org/10.1007/s11010-024-05110-0","url":null,"abstract":"Chronic obstructive pulmonary disease (COPD) is a deteriorating condition triggered by various factors, such as smoking, free radicals, and air pollution. This worsening disease is characterized by narrowing and thickening of airways, painful cough, and dyspnea. In COPD, numerous genes as well as microRNA (miRNA) play a significant role in the pathogenesis of the disease. Many in vivo and in vitro studies suggest that upregulation or suppression of certain miRNAs are effective treatment options for COPD. They have been proven to be more beneficial than the current symptomatic treatments, such as bronchodilators and corticosteroids. MiRNAs play a crucial role in immune cell development and regulate inflammatory responses in various tissues. MiRNA treatment thus allows for precision therapy with improved outcomes. Nanoparticle drug delivery systems such as polymeric nanoparticles, inorganic nanoparticles, dendrimers, polymeric micelles, and liposomes are an efficient method to ensure the biodistribution of the miRNAs to the target site. Identification of the right nanoparticle depending on the requirements and compatibility is essential for achieving maximum therapeutic effect. In this review, we offer a thorough comprehension of the pathology and genetics of COPD and the significance of miRNAs concerning various pathologies of the lung, as potential targets for treating the disease. The present review offers the latest insights into the nanoparticle drug delivery systems that can efficiently carry and deliver miRNA or antagomirs to the specific target site and hence help in effective management of COPD.<h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":4.3,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142203238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging insights into pulmonary hypertension: the potential role of mitochondrial dysfunction and redox homeostasis 肺动脉高压的新发现：线粒体功能障碍和氧化还原平衡的潜在作用

IF 4.3 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-10 DOI: 10.1007/s11010-024-05096-9

Junming Zhang, Huimin Yan, Yan Wang, Xian Yue, Meng Wang, Limin Liu, Pengfei Qiao, Yixuan Zhu, Zhichao Li

引用次数: 0

Endothelium Piezo1 deletion alleviates experimental varicose veins by attenuating perivenous inflammation. 内皮细胞 Piezo1 基因缺失可减轻静脉周围炎症，从而缓解实验性静脉曲张。

IF 3.5 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-09 DOI: 10.1007/s11010-024-05115-9

Jiani Zhao, Yacheng Xiong, Yu Liu, Jin Ling, Shuai Liu, Wei Wang

{"title":"Endothelium Piezo1 deletion alleviates experimental varicose veins by attenuating perivenous inflammation.","authors":"Jiani Zhao, Yacheng Xiong, Yu Liu, Jin Ling, Shuai Liu, Wei Wang","doi":"10.1007/s11010-024-05115-9","DOIUrl":"https://doi.org/10.1007/s11010-024-05115-9","url":null,"abstract":"Previous large-scale genetic studies have prioritized the causal genes piezo type mechanosensitive ion channel component 1 (PIEZO1) and castor zinc finger 1 (CASZ1) associated with varicose veins (VVs). This study aims to evaluate their roles in both clinical and experimental VVs. In this study, we investigated abundance of PIEZO1 and CASZ1 in both varicose and normal veins from the same patients. Yoda1 (a selective PIEZO1 agonist, 2.6 mg/kg/day) or vehicle was administered intraperitoneally for 3 weeks to evaluate the effect of PIEZO1 activation on experimental VVs. Subsequently, endothelial Piezo1 deletion mice (Piezo1iΔEC mice) were generated to explored the role of endothelial PIEZO1 on VVs. Laser speckle imaging, flow cytometry, cell tracing with Evans blue or rhodamine-6G, and histopathological staining were utilized to evaluate the pathophysiology of VVs. Our results showed that mRNA expression of PIEZO1, but not CASZ1, was abundant and increased in clinical VVs. The Piezo1tP1-td mice revealed endothelium-specific expression of PIEZO1 in mice veins. By establishing iliac vein ligation-induced VVs in mice, Yoda1 exacerbated experimental VVs with increased inflammatory cell infiltration. Subsequently, endothelial Piezo1 deletion (Piezo1iΔEC mice) alleviated experimental VVs and vascular remodeling by directly reducing vascular permeability and leukocyte-endothelium interactions compared to the control (Piezo1fl/fl mice). PIEZO1 is highly expressed in clinical VVs, meanwhile, activation or inhibition of PIEZO1 exerts a remarkable effect on experimental VVs. Furthermore, Piezo1 may constitute a potential therapeutic approach for the medical treatment of VVs.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholesterol suppresses AMFR-mediated PDL1 ubiquitination and degradation in HCC. 胆固醇可抑制 HCC 中 AMFR 介导的 PDL1 泛素化和降解。

IF 3.5 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-04 DOI: 10.1007/s11010-024-05106-w

Wei-Qing Shao, Yi-Tong Li, Xu Zhou, Sheng-Guo Zhang, Ming-Hao Fan, Dong Zhang, Zhen-Mei Chen, Chen-He Yi, Sheng-Hao Wang, Wen-Wei Zhu, Ming Lu, Ji-Song Chen, Jing Lin, Yu Zhou

{"title":"Cholesterol suppresses AMFR-mediated PDL1 ubiquitination and degradation in HCC.","authors":"Wei-Qing Shao, Yi-Tong Li, Xu Zhou, Sheng-Guo Zhang, Ming-Hao Fan, Dong Zhang, Zhen-Mei Chen, Chen-He Yi, Sheng-Hao Wang, Wen-Wei Zhu, Ming Lu, Ji-Song Chen, Jing Lin, Yu Zhou","doi":"10.1007/s11010-024-05106-w","DOIUrl":"https://doi.org/10.1007/s11010-024-05106-w","url":null,"abstract":"The degradation of proteasomes or lysosomes is emerging as a principal determinant of programmed death ligand 1 (PDL1) expression, which affects the efficacy of immunotherapy in various malignancies. Intracellular cholesterol plays a central role in maintaining the expression of membrane receptors; however, the specific effect of cholesterol on PDL1 expression in cancer cells remains poorly understood. Cholesterol starvation and stimulation were used to modulate the cellular cholesterol levels. Immunohistochemistry and western blotting were used to analyze the protein levels in the samples and cells. Quantitative real-time PCR, co-immunoprecipitation, and confocal co-localization assays were used for mechanistic investigation. A xenograft tumor model was constructed to verify these results in vivo. Our results showed that cholesterol suppressed the ubiquitination and degradation of PDL1 in hepatocellular carcinoma (HCC) cells. Further mechanistic studies revealed that the autocrine motility factor receptor (AMFR) is an E3 ligase that mediated the ubiquitination and degradation of PDL1, which was regulated by the cholesterol/p38 mitogenic activated protein kinase axis. Moreover, lowering cholesterol levels using statins improved the efficacy of programmed death 1 (PD1) inhibition in vivo. Our findings indicate that cholesterol serves as a signal to inhibit AMFR-mediated ubiquitination and degradation of PDL1 and suggest that lowering cholesterol by statins may be a promising combination strategy to improve the efficiency of PD1 inhibition in HCC.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142133202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated multi-omic high-throughput strategies across-species identified potential key diagnostic, prognostic, and therapeutic targets for atherosclerosis under high glucose conditions. 跨物种的综合多组学高通量策略确定了高血糖条件下动脉粥样硬化的潜在关键诊断、预后和治疗靶点。

IF 3.5 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-02 DOI: 10.1007/s11010-024-05097-8

Zhida Shen, Meng Zhao, Jiangting Lu, Huanhuan Chen, Yicheng Zhang, Songzan Chen, Zhaojing Wang, Meihui Wang, Xianglan Liu, Guosheng Fu, He Huang

{"title":"Integrated multi-omic high-throughput strategies across-species identified potential key diagnostic, prognostic, and therapeutic targets for atherosclerosis under high glucose conditions.","authors":"Zhida Shen, Meng Zhao, Jiangting Lu, Huanhuan Chen, Yicheng Zhang, Songzan Chen, Zhaojing Wang, Meihui Wang, Xianglan Liu, Guosheng Fu, He Huang","doi":"10.1007/s11010-024-05097-8","DOIUrl":"https://doi.org/10.1007/s11010-024-05097-8","url":null,"abstract":"Diabetes is a well-known risk factor for atherosclerosis (AS), but the underlying molecular mechanism remains unknown. The dysregulated immune response is an important reason. High glucose is proven to induce foam cell formation under lipidemia situations in clinical patients. Exploring the potential regulatory programs of accelerated foam cell formation stimulated by high glucose is meaningful. Macrophage-derived foam cells were induced in vitro, and high-throughput sequencing was performed. Coexpression gene modules were constructed using weighted gene co-expression network analysis (WGCNA). Highly related modules were identified. Hub genes were identified by multiple integrative strategies. The potential roles of selected genes were further validated in bulk-RNA and scRNA datasets of human plaques. By transfection of the siRNA, the role of the screened gene during foam cell formation was further explored. Two modules were found to be both positively related to high glucose and ox-LDL. Further enrichment analyses confirmed the association between the brown module and AS. The high correlation between the brown module and macrophages was identified and 4 hub genes (Aldoa, Creg1, Lgmn, and Pkm) were screened. Further validation in external bulk-RNA and scRNA revealed the potential diagnostic and therapeutic value of selected genes. In addition, the survival analysis confirmed the prognostic value of Aldoa while knocking down Aldoa expression alleviated the foam cell formation in vitro. We systematically investigated the synergetic effects of high glucose and ox-LDL during macrophage-derived foam cell formation and identified that ALDOA might be an important diagnostic, prognostic, and therapeutic target in these patients.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyses of oxidative DNA damage among coal vendors via single cell gel electrophoresis and quantification of 8-hydroxy-2'-deoxyguanosine. 通过单细胞凝胶电泳和 8-hydroxy-2'-deoxyguanosine 定量分析煤炭销售商的 DNA 氧化损伤。

IF 3.5 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-01 Epub Date: 2023-08-18 DOI: 10.1007/s11010-023-04826-9

Ali Sani, Ibrahim Lawal Abdullahi, Muhammad Idrees Khan, ChengXi Cao

{"title":"Analyses of oxidative DNA damage among coal vendors via single cell gel electrophoresis and quantification of 8-hydroxy-2'-deoxyguanosine.","authors":"Ali Sani, Ibrahim Lawal Abdullahi, Muhammad Idrees Khan, ChengXi Cao","doi":"10.1007/s11010-023-04826-9","DOIUrl":"10.1007/s11010-023-04826-9","url":null,"abstract":"Looking at the development status of Nigeria and other developing nations, most low-income and rural households often use coal as a source of energy which necessitates its trade very close to the communities. Moreover, the effects of exposure to coal mining activities are rarely explored or yet to be studied, not to mention the numerous street coal vendors in Nigeria. This study investigated the oxidative stress levels in serum and urine through the biomarker 8-OHdG and DNA damage via single cell gel electrophoresis (alkaline comet assay). Blood and urine levels of 8-OHdG from 130 coal vendors and 130 population-based controls were determined by ELISA. Alkaline comet assay was also performed on white blood cells for DNA damage. The average values of 8-OHdG in serum and urine of coal vendors were 22.82 and 16.03 ng/ml respectively, which were significantly greater than those detected in controls (p < 0.001; 15.46 and 10.40 ng/ml of 8-OHdG in serum and urine respectively). The average tail length, % DNA in tail and olive tail moment were 25.06 μm, 18.71% and 4.42 respectively for coal vendors. However, for controls, the average values were 4.72 μm, 3.63% and 1.50 for tail length, % DNA in tail and olive tail moment respectively which were much lower than coal vendors (p < 0.001). Therefore, prolonged exposure to coal dusts could lead to higher serum and urinary 8-OHdG and significant DNA damage in coal vendors observed in tail length, % DNA in tail, and olive tail moment by single cell gel electrophoresis. It is therefore established that coal vendors exhibit a huge risk from oxidative stress and assessment of 8-OHdG with single cell gel electrophoresis has proven to be a feasible tool as biomarkers of DNA damage.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10375197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sox17 protects human brain microvascular endothelial cells from AngII-induced injury by regulating autophagy and apoptosis. Sox17通过调节自噬和细胞凋亡保护人脑微血管内皮细胞免受AngII诱导的损伤。

IF 3.5 2区生物学

Molecular and Cellular Biochemistry Pub Date : 2024-09-01 Epub Date: 2023-09-02 DOI: 10.1007/s11010-023-04838-5

Yanyan Wang, Marong Fang, Qiannan Ren, Wei Qi, Xinli Bai, Nashwa Amin, Xiangjian Zhang, Zhenzhong Li, Lihong Zhang

{"title":"Sox17 protects human brain microvascular endothelial cells from AngII-induced injury by regulating autophagy and apoptosis.","authors":"Yanyan Wang, Marong Fang, Qiannan Ren, Wei Qi, Xinli Bai, Nashwa Amin, Xiangjian Zhang, Zhenzhong Li, Lihong Zhang","doi":"10.1007/s11010-023-04838-5","DOIUrl":"10.1007/s11010-023-04838-5","url":null,"abstract":"Intracranial aneurysm (IA), is a localized dilation of the intracranial arteries, the rupture of which is catastrophic. Hypertension is major IA risk factor that mediates endothelial cell damage. Sox17 is highly expressed in intracranial vascular endothelial cells, and GWAS studies indicate that its genetic alteration is one of the major genetic risk factors for IA. Vascular endothelial cell injury plays a vital role in the pathogenesis of IA. The genetic ablation of Sox17 plus hypertension induced by AngII can lead to an increased incidence of intracranial aneurysms had tested in the previous animal experiments. In order to study the underlying molecular mechanisms, we established stable Sox17-overexpressing and knockdown cell lines in human brain microvascular endothelial cells (HBMECs) first. Then flow cytometry, western blotting, and immunofluorescence were employed. We found that the knockdown of Sox17 could worsen the apoptosis and autophagy of HBMECs caused by AngII, while overexpression of Sox17 had the opposite effect. Transmission electron microscopy displayed increased autophagosomes after the knockdown of Sox17 in HBMECs. The RNA-sequencing analysis shown that dysregulation of the Sox17 gene was closely associated with the autophagy-related pathways. Our study suggests that Sox17 could protect HBMECs from AngII-induced injury by regulating autophagy and apoptosis.","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10131370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0