Molecular and Cellular Biochemistry最新文献

{"title":"Vitamin D alleviates oxidative stress in varicose veins: a pilot study in obese and non-obese patients.","authors":"Sonia Rațiu, Mihaela I Mariș, Adina V Furdui-Lința, Theia Stanciu-Lelcu, Claudia Borza, Sorin Olariu, Tiberiu Bratu, Adrian Sturza, Danina M Muntean","doi":"10.1007/s11010-025-05292-1","DOIUrl":"10.1007/s11010-025-05292-1","url":null,"abstract":"<p><p>Chronic venous disease and varicose veins of the lower extremities represent a widespread pathology, particularly in individuals with obesity. A high prevalence of varicose vein disease has been observed in obese patients in association with lower plasma levels of vitamin D. The present pilot study aimed to investigate the acute effects of 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃], the biologically active form of vitamin D, on oxidative stress in varicose veins obtained from both obese and non-obese patients undergoing cryostripping surgery for varicose vein ablation. Varicose venous samples treated or not with 1,25(OH)₂D₃ (100 nM, 12-h incubation) were analysed for reactive oxygen species (ROS) generation using the ferrous xylenol orange oxidation (FOX) assay and immunofluorescence technique. Additionally, the gene expression of endothelial nitric oxide synthase (eNOS), neuronal nitric oxide synthase (nNOS), and inducible nitric oxide synthase (iNOS) was assessed via qPCR. We report a significant reduction in circulating 25-hydroxyvitamin D₃ [25(OH)D₃] levels in obese as compared to non-obese patients. Ex vivo incubation of the venous samples with 1,25(OH)₂D₃ resulted in: (i) significant reduction in ROS level, (ii) upregulation of eNOS and nNOS expression, and (iii) downregulation of iNOS expression in both groups of patients. Vitamin D did not exhibit a ROS scavenger effect, and the antioxidant effect is presumably mediated via its receptor whose presence was confirmed in the varicose venous samples. In conclusion, vitamin D exerts protective effects in venous pathology, which may be beneficial in acute administration prior to the surgical intervention. Large clinical trials are required to assess the optimal dosage and time/duration of administration in patients with chronic venous disease with surgical indication.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":"4797-4807"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12316817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of resveratrol on inflammatory cytokines in COVID-19 patients: a randomized, double-blinded, placebo-controlled clinical trial.","authors":"Alireza Bastin, Thomas Netticadan, Fatemeh Abbasi, Naseeb Rahimi, Marzieh Mahmoodi, Mehdi Alizadeh, Ali Movahed, Mohammad Amin Momeni-Moghaddam","doi":"10.1007/s11010-025-05290-3","DOIUrl":"10.1007/s11010-025-05290-3","url":null,"abstract":"<p><p>Studies show that the clinical symptoms of Coronavirus disease 2019 (COVID-19) are significantly reduced by treatment with medicinal plants, including plants with strong anti-inflammatory and antioxidant properties. The use of herbal medicines and supplements is considered to be suitable due to relatively mild side effects. Resveratrol is one of the most potent plant compounds found in both medicinal and non-medicinal plants, and it has strong anti-inflammatory and antioxidant properties. Therefore, the aim of the double-blind clinical study was to investigate the effects of resveratrol consumption on biochemical markers, hematological parameters, and some inflammatory cytokines in patients with COVID-19.</p><p><strong>Methods: </strong>A total of 44 patients with COVID-19 were randomly assigned to receive 750 mg/day of resveratrol (n = 24) orally or placebo (n = 20) for 10 days. A permuted block randomized design (block size two) was used for randomization. The biochemical markers, hematological parameters, and plasma levels of Interleukin-1 beta (IL-1β), Interleukin-6 (IL-6), Interleukin-10 (IL-10), and Tumor necrosis factor-alpha (TNF-α) cytokines were measured at baseline and after 10 days.</p><p><strong>Results: </strong>We observed a statistically significant reduction in the C-reactive protein (CRP) (P = 0.041), Fasting blood sugar (FBS) (P = 0.002), Alkaline phosphatase (ALP) (P = 0.034), IL-1β (P = 0.011), TNF-α (P = 0.001), and White blood cell (WBC) (P = 0.043), Platelet count (PLT) (P = 0.042), Neutrophils (NUT) (P = 0.015) and an increase in Lymphocyte (LYM) (P = 0.010) in the resveratrol treated group when compared with the placebo group.</p><p><strong>Conclusion: </strong>The present study demonstrated that resveratrol as an herbal supplement may be useful in reducing markers of inflammation, neutrophils, and low platelet count, as well as lowering blood glucose levels in patients with COVID-19. The trial was prospectively registered at IRCT.ir as IRCT20111119008129N13. Registration date: 2022-10-11.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":"4865-4872"},"PeriodicalIF":3.7,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sirtuins in mitophagy: key gatekeepers of mitochondrial quality.","authors":"Francisco Alejandro Lagunas-Rangel","doi":"10.1007/s11010-025-05358-0","DOIUrl":"https://doi.org/10.1007/s11010-025-05358-0","url":null,"abstract":"<p><p>Mitochondria are highly dynamic organelles essential for cellular energy production. However, they are also a primary source of reactive oxygen species, making them particularly vulnerable to oxidative damage. To preserve mitochondrial integrity, cells employ quality control mechanisms such as mitophagy, a selective form of autophagy that targets damaged or dysfunctional mitochondria for degradation. Among the key regulators of mitophagy are the sirtuins, a family of NAD⁺-dependent deacetylases. SIRT1, SIRT3, and SIRT6 generally promote mitophagy, whereas SIRT2, SIRT4, SIRT5, and SIRT7 often act as negative regulators. Sirtuin-mediated regulation of mitophagy is critical for maintaining cellular homeostasis and is implicated in a variety of physiological and pathological conditions. The aim of this review is to provide an overview focused on describing how sirtuins influence the mitophagy process. It highlights the different molecular mechanisms by which individual members of the sirtuin family modulate mitophagy, either by promoting or suppressing it, depending on the context. In addition, the review explores the relevance of sirtuin-regulated mitophagy in health and disease, emphasizing some conditions under which altered sirtuin activity could be harnessed for therapeutic benefit.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of extracellular vesicles derived from cortical bone stem cells compared with mesenchymal stem cells.","authors":"Norihiko Sasaki, Kyojiro Kawakami, Yoko Itakura, Takayuki Fujita, Shigeru Miyagawa, Tomoaki Ishigami, Hajime Kubo, Yuri Miura, Yumi Chiba","doi":"10.1007/s11010-025-05348-2","DOIUrl":"https://doi.org/10.1007/s11010-025-05348-2","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are considered useful therapeutic tools in regenerative medicine. They are an alternative to stem cells and drug delivery vehicles, but their properties differ depending on their cell type of origin. Previously, we reported differences in the cellular properties between mouse cortical bone-derived stem cells (mCBSCs) and mouse mesenchymal stem cells (mMSCs). In this study, we aimed to clarify the characteristics of mCBSC-derived EVs (mCBSC-EVs), whose properties remain unclear, and compared them with those of mMSC-EVs. Proteomic analysis identified 3470 proteins in both EVs, of which 224 and 217 were unique to mCBSC-EVs and mMSC-EVs, respectively. Glycomic analysis using lectin microarrays showed significant differences in the relative intensities of various types of lectins between mCBSC-EVs and mMSC-EVs. Specifically, fucosylated glycans, sialic acid structures, and glycans with bisecting N-acetylglucosamine were differentially expressed between mCBSC-EVs and mMSC-EVs. Finally, we examined the uptake and functional effects of EVs on mouse endothelial cells (mECs). mECs preferentially took up mCBSC-EVs than mMSC-EVs. Moreover, mCBSC-EVs resulted in greater upregulation of adhesion and inflammatory molecules in response to TNF-α stimulation than did mMSC-EVs. Furthermore, both mCBSC-EVs and mMSC-EVs elicited comparable levels of tube-like structure formation by mECs. Taken together, our results revealed that mCBSC-EVs and mMSC-EVs exhibit differences in cargo contents and biological action, suggesting the predominance of mCBSC-EVs. Therefore, CBSC-EVs may be useful for treating various physiological and pathological conditions, including wound healing and angiogenesis in ischemic diseases.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144699019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerebrospinal fluid sphingolipid and sterol metabolite profiles of neurologically normal subjects.","authors":"Yoshiki Niimi, Akihiro Ueda, Hisako Akiyama, Ryoichi Shiroki, Peter Greimel, Yoshio Hirabayashi, Kiyotaka Hoshinaga, Tatsuro Mutoh","doi":"10.1007/s11010-025-05334-8","DOIUrl":"https://doi.org/10.1007/s11010-025-05334-8","url":null,"abstract":"<p><p>Although glycosphingolipids (GSLs) and sterol metabolites are known to be involved in many diseases, including neurodegenerative disorders, the quantification of these molecules in humans has been scarcely investigated. Similarly, the effects of age, sex, and statin use have not been sufficiently investigated or reported in the literature. In this study, we measured the levels of human cerebrospinal fluid (CSF) GSLs of various fatty acid acyl chain lengths and sterol metabolites using hydrophilic interaction liquid chromatography and electrospray tandem mass spectrometry in a neurologically normal population. We successfully quantified the presence of glucosylated cholesterol, galactosylated cholesterol, glucosylated sitosterol, glucosylceramide (GlcCer), galactosylceramide, lactosylceramide (LacCer), and galabiosylceramide (Gb2). There were no statistically significant differences in CSF concentrations of these lipids between males and females. We also found no correlation between CSF concentration and age or statin dose, except for GlcCer d18:1-C23:1. Significant positive correlations with age were shown only in males. Our results indicate that, in future studies, age and sex should be taken into consideration when comparing CSF GSL levels in patients with neurological disorders with those in neurologically normal control subjects.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of TMAO on aging-associated cardiovascular and metabolic pathways and emerging therapies.","authors":"Manal Alsulami, Hassan Alamri, Tlili Barhoumi, Nayla Munawar, Bandar Alghanem","doi":"10.1007/s11010-025-05356-2","DOIUrl":"https://doi.org/10.1007/s11010-025-05356-2","url":null,"abstract":"<p><p>Metabolites produced by gut microbes have been linked to the pathophysiology of several disorders, including cardiovascular disease (CVD), such as atherosclerosis, hypertension, heart failure, and myocardial infarction. Trimethylamine-N-oxide (TMAO) is a primary diet-induced metabolite generated by the gut microbiota. TMAO is involved in dysregulation of bile acid and cholesterol metabolism, which promotes oxidative stress, inflammation, and endothelial dysfunction leading to vascular damages. Aging is usually associated with cardio-metabolic diseases, and the increasing evidence reveals that TMAO is linked with an increased risk of CVD in elderly. However, the exact relationship between plasma TMAO levels, the risk of CVD, and aging remains unclear. In this review, we explore the three-dimensional relationship between TMAO, aging, and CVD. Moreover, we discussed the new therapies used to reduce TMAO levels in aging and CVD.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Balancing senescence and apoptosis: therapeutic insights into aging and cancer.","authors":"Nusrat Jan, Shazia Sofi, Aijaz Ahmad Mir, Gowhar Masoodi, Manzoor Ahmad Mir","doi":"10.1007/s11010-025-05355-3","DOIUrl":"10.1007/s11010-025-05355-3","url":null,"abstract":"<p><p>Aging and cancer are interconnected processes resulting from irreversible dysfunctions, primarily caused by stress-induced DNA and cellular damage. While aging is characterized by the accumulation of senescent cells (SnCs), cancer development is linked to a progressive decline in the ability of cells to undergo senescence and apoptosis. Both processes serve as crucial tumor suppressor mechanisms in early life but may contribute to aging-related pathologies over time. Cellular senescence and apoptosis are stress responses that play vital roles in maintaining tissue homeostasis. In aging, SnCs accumulate in tissues due to dysregulated apoptotic pathways, while in cancer, reduced senescence and apoptosis enable uncontrolled proliferation. Recent findings suggest that several anticancer drugs, including classical apoptosis inducers, can also promote senescence, highlighting the potential for pro-senescence strategies in cancer therapy. Understanding the mechanistic pathways and biomarkers of these processes can provide insight into their interplay and their impact on disease progression. Exploring the balance between cellular senescence and apoptosis may lead to novel therapeutic approaches for both aging and cancer. Targeting these mechanisms could help develop anti-aging and anticancer treatments with minimized adverse effects, offering promising avenues for future research and clinical applications.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.7,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LINC02266 promotes proliferation and metastasis and inhibits ferroptosis of gastric cancer cells by regulating AKT/ACSL4 pathway.","authors":"Xiaoru Liang, Xinyu Niu, Lijie Zhang, Chang Liu, Xiaomin Zhao, Feng Chen, Zhijuan Lin","doi":"10.1007/s11010-025-05354-4","DOIUrl":"https://doi.org/10.1007/s11010-025-05354-4","url":null,"abstract":"<p><p>Ferroptosis is a novel form of cell death characterized by iron-dependent oxidative damage, lipid peroxidation, and the accumulation of reactive oxygen species. There exists a close correlation between ferroptosis and gastric cancer (GC). Recent research has shown that many ferroptosis-related long non-coding RNAs (lncRNAs) affect the occurrence and progression of GC. However, the exact mechanism has not been elucidated. This study showed that a novel lncRNA LINC02266 was upregulated in GC cells and was a key lncRNA associated with GC prognosis and progression. Functional analysis indicated that LINC02266 suppressed ferroptosis and promoted GC cell proliferation, migration, invasion, and tumor growth in vitro and vivo. Mechanistically, we found that LINC02266 inhibited erastin-induced ferroptosis by suppressing ACSL4 levels, which may be related to the AKT pathway. This study indicates that LINC02266 can serve as a diagnostic marker and novel therapeutic target for GC.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise and its beneficial impacts on mood disorders and neurodegeneration: a novel mechanistic perspective of the exosomes involvement.","authors":"Jingwen Wang, Shiqian Fan, Jianshe Wang","doi":"10.1007/s11010-025-05357-1","DOIUrl":"https://doi.org/10.1007/s11010-025-05357-1","url":null,"abstract":"<p><p>Mood disorders (MDs), including depression and anxiety, are among the leading causes of global disability, yet current treatments often yield incomplete remission. Women are disproportionately affected, underscoring the need for more targeted and effective interventions. Emerging evidence suggests that small extracellular vesicles (sEVs), particularly exosomes released during aerobic exercise, may mediate its antidepressant effects by facilitating inter-organ communication and delivering neuroactive cargo, such as microRNAs, across the blood-brain barrier. These vesicles influence synaptic plasticity, neurogenesis, and inflammation, offering both mechanistic insight and biomarker potential. This review proposes that exercise-induced exosomes are key modulators of brain health and mood regulation. By synthesizing findings from human and animal studies, we explore how aerobic exercise promotes neural resilience through exosomal signaling, with attention to sex-specific hormonal influences. Understanding these pathways may inform precision-based, non-pharmacological strategies for managing mood disorders and mitigating neurodegenerative risk.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol improves lipid profile and recovers heart function in postoperative CABG patients.","authors":"Parisa Ahmadi, Samad Akbarzadeh, Mehdi Alizadeh, Neshat Mohammadi, Marzieh Mahmoodi, Azam Amini, Thomas Netticadan, Mikaeil Rezaei, Ali Movahed","doi":"10.1007/s11010-025-05351-7","DOIUrl":"https://doi.org/10.1007/s11010-025-05351-7","url":null,"abstract":"<p><p>Cardiovascular diseases (CVD), especially coronary artery disease (CAD), are among the most important causes of mortality in the world. Coronary artery bypass grafting (CABG) is a standard treatment for this disease, but it may be associated with complications in the postoperative period. This study investigated the effect of resveratrol (RES), a potent antioxidant and anti-inflammatory molecule, on cardiovascular indices and oxidative stress in patients after CABG surgery. This randomized, double-blind, placebo-controlled trial was carried out at the Bushehr Heart Center in Bushehr, Iran. A total of 60 patients undergoing CABG were randomly assigned in equal numbers to either the intervention group or the control group. The intervention group received RES capsules for 60 days, and the control group received a placebo for the same duration. Cardiovascular function and biochemical parameters, including lipid profile, cardiac enzymes, and markers of oxidative stress and inflammation, were measured at the beginning and end of the intervention. Compared to the control group, RES intake significantly reduced systolic blood pressure (SBP), triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL), and improved ejection fraction (EF). Although decreases in cardiac enzymes, including creatine phosphokinase (CPK) and lactate dehydrogenase (LDH), were observed in the RES group, these changes were not statistically significant. Also, the two groups showed no significant differences in diastolic blood pressure (DBP), HDL, or oxidative stress markers. RES supplementation protects patients after CABG by improving cardiovascular function and lipid profiles.Trial registration: IRCT20111119008129N12, first trial registration date: 2022-06-18.</p>","PeriodicalId":18724,"journal":{"name":"Molecular and Cellular Biochemistry","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}