Methods and Protocols最新文献

eDNA-Based Survey of Fish Species in Water Bodies Using Loop-Mediated Isothermal Amplification (LAMP) for Application of Developing Automatic Sampler. 利用环路介导等温扩增法（LAMP）进行基于 eDNA 的水体鱼类物种调查，以应用正在开发的自动采样器。

IF 2.3

Methods and Protocols Pub Date : 2024-10-23 DOI: 10.3390/mps7060085

Nivedhitha Jothinarayanan, Chau Ha Pham, Frank Karlsen, Lars Eric Roseng

{"title":"eDNA-Based Survey of Fish Species in Water Bodies Using Loop-Mediated Isothermal Amplification (LAMP) for Application of Developing Automatic Sampler.","authors":"Nivedhitha Jothinarayanan, Chau Ha Pham, Frank Karlsen, Lars Eric Roseng","doi":"10.3390/mps7060085","DOIUrl":"https://doi.org/10.3390/mps7060085","url":null,"abstract":"The monitoring of species in a habitat is important to ensure biological diversity. Environmental DNA (eDNA) can infer the presence-absence of species and enable rapid action to avoid threatening factors in ecosystems in the case of non-indigenous species. Loop-mediated isothermal amplification (LAMP) assays for molecular amplification are rapidly gaining popularity in species detection, but LAMP remains an underutilized method for eDNA-based monitoring practices. The most effective combination for successful species monitoring may be the collection of eDNA or biological traces collected by nanofiltration followed by LAMP-based species detection initiatives. Here, we used LAMP analysis to detect the eDNA of Esox lucius (Northern Pike), Anguilla anguilla (European Eel), and Salmo salar (Atlantic Salmon) in Borre Lake and in the Drammen River. The selection of species is based on the categories of regionally invasive species, endangered species, and species of least concern. Two target genes were considered for each species and LAMP primers were designed. Our study showed that LAMP is an effective tool for discovering specific fish eDNA (analysis) to maintain aquatic ecosystems.","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity Identification, Classification, and Representation of Wheelchair Sport Court Tasks: A Method Proposal. 轮椅运动场任务的活动识别、分类和表示：方法建议。

IF 2.3

Methods and Protocols Pub Date : 2024-10-18 DOI: 10.3390/mps7050084

Mathieu Deves, Christophe Sauret, Ilona Alberca, Lorian Honnorat, Yoann Poulet, Arnaud Hays, Arnaud Faupin

{"title":"Activity Identification, Classification, and Representation of Wheelchair Sport Court Tasks: A Method Proposal.","authors":"Mathieu Deves, Christophe Sauret, Ilona Alberca, Lorian Honnorat, Yoann Poulet, Arnaud Hays, Arnaud Faupin","doi":"10.3390/mps7050084","DOIUrl":"https://doi.org/10.3390/mps7050084","url":null,"abstract":"Background: Monitoring player mobility in wheelchair sports is crucial for helping coaches understand activity dynamics and optimize training programs. However, the lack of data from monitoring tools, combined with a lack of standardized processing approaches and ineffective data presentation, limits their usability outside of research teams. To address these issues, this study aimed to propose a simple and efficient algorithm for identifying locomotor tasks (static, forward/backward propulsion, pivot/tight/wide rotation) during wheelchair movements, utilizing kinematic data from standard wheelchair mobility tests.Methods: Each participant's wheelchair was equipped with inertial measurement units-two on the wheel axes and one on the frame. A total of 36 wheelchair tennis and badminton players completed at least one of three proposed tests: the star test, the figure-of-eight test, and the forward/backward test. Locomotor tasks were identified using a five-step procedure involving data reduction, symbolic approximation, and logical pattern searching.Results: This method successfully identified 99% of locomotor tasks for the star test, 95% for the figure-of-eight test, and 100% for the forward/backward test.Conclusion: The proposed method offers a valuable tool for the simple and clear identification and representation of locomotor tasks over extended periods. Future research should focus on applying this method to wheelchair court sports matches and daily life scenarios.","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validity and Reliability According to the Type of Examiners in the Process of Calibrating Dental Caries Experience Using the DMFT Index. 在使用 DMFT 指数校准龋齿经验的过程中，根据考官类型确定有效性和可靠性。

IF 2.3

Methods and Protocols Pub Date : 2024-10-16 DOI: 10.3390/mps7050083

Anna Paola Fernández-Coll, María Claudia Garcés-Elías, Jorge A Beltrán, Roberto A León-Manco, Janett Mas-López

{"title":"Validity and Reliability According to the Type of Examiners in the Process of Calibrating Dental Caries Experience Using the DMFT Index.","authors":"Anna Paola Fernández-Coll, María Claudia Garcés-Elías, Jorge A Beltrán, Roberto A León-Manco, Janett Mas-López","doi":"10.3390/mps7050083","DOIUrl":"https://doi.org/10.3390/mps7050083","url":null,"abstract":"The process of examiner calibration is an essential step in all epidemiological research, as it aims to ensure uniform interpretation, understanding, and application of the instrument to be used. This ensures that the data collected will be valid and reliable. This study aimed to determine the differences in concordance in dental caries calibration across three dental specialties. The population consisted of 45 dentists, divided into three groups: 15 general dentists working in the public sector, 15 dentists specializing in Dental Public Health, and 15 dentists specializing in Restorative and Aesthetic Dentistry. The calibration process was carried out in three stages: theory, calibration using photographs, and calibration on natural teeth, performed by the gold standard. In the first validity process, a statistical difference was only found between the Kappa values of the inter-examiner calibration process using photographs. For the evaluation of teeth, in the second validity process, 33.33% (n = 15) of the participants achieved \"almost perfect agreement.\" Finally, only 75.56% (n = 34) of the examiners were considered for the reliability report; of this group, 52.94% (n = 18) were in \"almost perfect agreement,\" and 35.29% (n = 12) were in \"substantial agreement.\" The validity and reliability of the dental caries experience calibration process did not present significant statistical differences between general dentists in the public sector, dentists specializing in Dental Public Health, and dentists specializing in Restorative and Aesthetic Dentistry.","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Versatile Ultra-High-Performance Liquid Chromatography-Full-Scan High-Resolution Mass Spectrometry Method to Quantify Wine Polyphenols. 多功能超高效液相色谱-全扫描高分辨率质谱法定量葡萄酒多酚。

IF 2.3

Methods and Protocols Pub Date : 2024-10-10 DOI: 10.3390/mps7050082

Damien Flores, Emmanuelle Meudec, Aécio Luís de Sousa Dias, Nicolas Sommerer

引用次数: 0

Evaluating Age-Friendly Health Care Approaches in Rural Primary Care Settings: A Multi-Case, Mixed-Methods Hybrid Type 2 Effectiveness-Implementation Study. 评估农村初级医疗机构中的老年友好型医疗保健方法：多案例、混合方法混合型 2 类有效性-实施研究》（A Multi-Case, Mixed-Methods Hybrid Type 2 Effectiveness-Implementation Study）。

IF 2.3

Methods and Protocols Pub Date : 2024-10-08 DOI: 10.3390/mps7050081

Kathleen Brasher, Rachel Winterton, Clare Wilding, Kelly Tamang

引用次数: 0

Effects of Sucralose Supplementation on Glycemic Response, Appetite, and Gut Microbiota in Subjects with Overweight or Obesity: A Randomized Crossover Study Protocol. 补充三氯蔗糖对超重或肥胖受试者血糖反应、食欲和肠道微生物群的影响：随机交叉研究方案。

IF 2.3

Methods and Protocols Pub Date : 2024-10-07 DOI: 10.3390/mps7050080

Zeniff Reyes-López, Viridiana Olvera-Hernández, Meztli Ramos-García, José D Méndez, Crystell G Guzmán-Priego, Miriam C Martínez-López, Carlos García-Vázquez, Carina S Alvarez-Villagomez, Isela E Juárez-Rojop, Juan C Díaz-Zagoya, Jorge L Ble-Castillo

{"title":"Effects of Sucralose Supplementation on Glycemic Response, Appetite, and Gut Microbiota in Subjects with Overweight or Obesity: A Randomized Crossover Study Protocol.","authors":"Zeniff Reyes-López, Viridiana Olvera-Hernández, Meztli Ramos-García, José D Méndez, Crystell G Guzmán-Priego, Miriam C Martínez-López, Carlos García-Vázquez, Carina S Alvarez-Villagomez, Isela E Juárez-Rojop, Juan C Díaz-Zagoya, Jorge L Ble-Castillo","doi":"10.3390/mps7050080","DOIUrl":"https://doi.org/10.3390/mps7050080","url":null,"abstract":"Sucralose stands as the most common non-nutritive sweetener; however, its metabolic effects have sparked significant controversy over the years. We aim to examine the effects of sucralose daily intake on glycemia, subjective appetite, and gut microbiota (GM) changes in subjects with overweight or obesity. In this randomized, crossover, and controlled trial, 23 participants with a body mass index between 25 kg/m2 and 39.9 kg/m2 will be assigned to one of two interventions to receive either sucralose (2 mg/kg/day equivalent to 40% of the acceptable daily intake) or glucose (control) for 4 weeks, each phase separated by a 4-week washout period. The glycemic response will be determined during a meal tolerance test, subjective appetite will be evaluated using a visual analog scale, and GM changes will be analyzed by next-generation sequencing of the bacterial rRNA 16S gene from fecal samples. All measures will be performed before and after intervention periods. We hypothesize that sucralose supplementation induces changes in glycemic response, subjective appetite, and gut microbiota in overweight and obese participants. This protocol was approved by the Ethics Committee of the UJAT (No. 0721) and was registered in the Australian New Zealand Clinical Trials Registry (ACTRN12621001531808).","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prophylactic Administration of Perampanel for Post-Stroke Epilepsy (PROPELLER Study): A Trial Protocol. 中风后癫痫预防性用药 Perampanel（PROPELLER 研究）：试验方案。

IF 2.3

Methods and Protocols Pub Date : 2024-10-05 DOI: 10.3390/mps7050079

Shuichi Yamada, Ichiro Nakagawa, Masashi Kotsugi, Kiyoshi Asada, Masato Kasahara

{"title":"Prophylactic Administration of Perampanel for Post-Stroke Epilepsy (PROPELLER Study): A Trial Protocol.","authors":"Shuichi Yamada, Ichiro Nakagawa, Masashi Kotsugi, Kiyoshi Asada, Masato Kasahara","doi":"10.3390/mps7050079","DOIUrl":"https://doi.org/10.3390/mps7050079","url":null,"abstract":"Background: Post-stroke epilepsy can reduce patients' abilities to carry out various activities of daily living. Despite their importance in preventing the onset of post-stroke epilepsy, the prophylactic administration of antiepileptic drugs is controversial due to a lack of high-level clinical research. In this study, we initiated a prospective interventional study of prophylactic antiepileptic drug administration in patients with a subcortical hemorrhage, who are at the highest risk of developing epilepsy after experiencing a stroke.Methods: The study was conducted in a single-center setting and was a single-arm study with no control group; the case entry period started in November 2023 and is due to end in March 2025. Only cases with a subcortical hemorrhage will be included. The treatment regimen used in this study is 2 mg of perampanel per day. Perampanel will be administered for one year, followed by two years of follow-up, for a total study period of three years. The primary endpoint will be the development of epilepsy.Results: Perampanel administration is expected to reduce the incidence of post-stroke epilepsy in comparison to the results of previous reports on the use of alternative treatments.Conclusions: The results of this study will provide new insights into the prevention of post-stroke epilepsy. The relatively small size of this study makes it difficult to provide strong evidence of the efficacy of perampanel, but it may serve as a basis for larger clinical trials.","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Telenursing on Supportive Care Needs in Patients with Melanoma and Lung Cancer on Targeted Therapies: A Randomised Controlled Trial Study Protocol. 远程护理对接受靶向治疗的黑色素瘤和肺癌患者辅助护理需求的影响：随机对照试验研究方案》。

IF 2.3

Methods and Protocols Pub Date : 2024-10-03 DOI: 10.3390/mps7050078

Aurora De Leo, Gloria Liquori, Alessandro Spano, Nicolò Panattoni, Sara Dionisi, Laura Iacorossi, Noemi Giannetta, Irene Terrenato, Emanuele Di Simone, Marco Di Muzio, Fabrizio Petrone

{"title":"Effect of Telenursing on Supportive Care Needs in Patients with Melanoma and Lung Cancer on Targeted Therapies: A Randomised Controlled Trial Study Protocol.","authors":"Aurora De Leo, Gloria Liquori, Alessandro Spano, Nicolò Panattoni, Sara Dionisi, Laura Iacorossi, Noemi Giannetta, Irene Terrenato, Emanuele Di Simone, Marco Di Muzio, Fabrizio Petrone","doi":"10.3390/mps7050078","DOIUrl":"https://doi.org/10.3390/mps7050078","url":null,"abstract":"Background: Telenursing comprises a set of tools and interventions enabling nurses to provide remote care. This study aims to assess the impact of telenursing interventions on the supportive care needs of patients with melanoma and lung cancer who are receiving targeted therapies. Methods: This six-month monocentric, double-arm, randomised, controlled trial study protocol will assess the effect of telenursing on the supportive care needs (primary outcome) in 40 patients (20 in each group) after one month. The secondary outcomes will be monitored at baseline, one, three and six months: supportive care needs (at three and six months), therapeutic adherence, quality of life, usability and satisfaction, performance status, patient-reported outcomes and main adverse events. The SPIRIT guidelines will be used for the reporting. Results: The results from this trial will assess the impact of a telenursing intervention on cancer care. Conclusions: This trial could be a starting point for more extensive studies on telenursing interventions to promote nurses' skills, as well as the quality and safety of care in patients with cancer, highlighting the impact of more outstanding nursing contributions on cancer care. Trial and Protocol Registration: The study protocol was approved by the relevant Italian Ethics Committee Lazio Area 5 (RS1851/23, 2773; 6 September 2023) and was registered on ClinicalTrials.gov (trial registry number NCT06254196).","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral Pre-Exposure Prophylaxis Innovative Interventions among Adolescent Girls and Young Women in South Africa: A Protocol Paper. 南非少女和年轻妇女口服暴露前预防创新干预措施：协议文件。

IF 2.3

Methods and Protocols Pub Date : 2024-09-29 DOI: 10.3390/mps7050077

Lerato Lucia Olifant, Edith Phalane, Refilwe Nancy Phaswana-Mafuya

{"title":"Oral Pre-Exposure Prophylaxis Innovative Interventions among Adolescent Girls and Young Women in South Africa: A Protocol Paper.","authors":"Lerato Lucia Olifant, Edith Phalane, Refilwe Nancy Phaswana-Mafuya","doi":"10.3390/mps7050077","DOIUrl":"https://doi.org/10.3390/mps7050077","url":null,"abstract":"Although South Africa was the first country to register and roll out oral pre-exposure prophylaxis (PrEP) biomedical human immunodeficiency virus (HIV) prevention intervention in sub-Saharan Africa (SSA), its uptake remains low, particularly among adolescent girls and young women (AGYW). The uptake of PrEP may have worsened during the Coronavirus disease 2019 (COVID-19) pandemic. Some innovative interventions to improve PrEP uptake among AGYW have been implemented. This study aims to evaluate the effectiveness of PrEP innovative interventions implemented during COVID-19 towards reducing the risk of HIV infection among AGYW in South Africa. An exploratory, descriptive design will be conducted to carry out four study objectives. Firstly, to carry out a systematic review of innovative PrEP interventions implemented during COVID-19 in SSA countries. Secondly, to conduct a stakeholder analysis to identify PrEP stakeholders and interview them on their views on the implemented interventions. Thirdly, to assess the implementation outcomes of the innovative interventions using document reviews and Consolidated Framework for Implementation Research. Fourthly, to develop a framework for an improved PrEP service delivery among AGYW. Qualitative data will be captured in ATLAS.ti software (Technical University, Berlin, Germany) version 23 and analysed via thematic analysis. A statistical software package (STATA) version 18 (College Station, TX, USA) will be used to capture quantitative data and analyse them via descriptive analysis. The generated evidence will be used towards the development of framework, guidelines, and policies to strengthen the uptake of, scale-up, and adherence to PrEP among AGYW.","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510152/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Decellularized Bio-Scaffolds for the Generation of a Porcine Artificial Intestine. 利用脱细胞生物支架生成猪人工肠道

IF 2.3

Methods and Protocols Pub Date : 2024-09-27 DOI: 10.3390/mps7050076

Sharon Arcuri, Georgia Pennarossa, Madhusha Prasadani, Fulvio Gandolfi, Tiziana A L Brevini

{"title":"Use of Decellularized Bio-Scaffolds for the Generation of a Porcine Artificial Intestine.","authors":"Sharon Arcuri, Georgia Pennarossa, Madhusha Prasadani, Fulvio Gandolfi, Tiziana A L Brevini","doi":"10.3390/mps7050076","DOIUrl":"https://doi.org/10.3390/mps7050076","url":null,"abstract":"In recent years, great interest has been focused on the development of highly reproducible 3D in vitro models that are able to mimic the physiological architecture and functionality of native tissues. To date, a wide range of techniques have been proposed to recreate an intestinal barrier in vitro, including synthetic scaffolds and hydrogels, as well as complex on-a-chip systems and organoids. Here, we describe a novel protocol for the generation of an artificial intestine based on the creation of decellularized bio-scaffolds and their repopulation with intestinal stromal and epithelial cells. Organs collected at the local slaughterhouse are subjected to a decellularization protocol that includes a freezing/thawing step, followed by sequential incubation in 1% SDS for 12 h, 1% Triton X-100 for 12 h, and 2% deoxycholate for 12 h. At the end of the procedure, the generated bio-scaffolds are repopulated with intestinal fibroblasts and then with epithelial cells. The protocol described here represents a promising and novel strategy to generate an in vitro bioengineered intestine platform able to mimic some of the complex functions of the intestinal barrier, thus constituting a promising 3D strategy for nutritional, pharmaceutical, and toxicological studies.","PeriodicalId":18715,"journal":{"name":"Methods and Protocols","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11510128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0