小鼠胰腺腺癌两种磁性三维球体模型的建立与表征。

IF 2 Q3 BIOCHEMICAL RESEARCH METHODS
Magali Perier, Litan Wang, Marine Simonneau, Jacqueline Ngo-Reymond, Julie Guillermet-Guibert, Maxime Lafond, Cyril Lafon
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引用次数: 0

摘要

胰腺腺癌仍然是最致命的癌症之一,治疗选择有限,耐药程度高。传统的二维细胞培养不能准确地复制肿瘤结构。我们的研究引入了三维(3D)胰腺腺癌球体模型,利用胰腺癌细胞和永生化成纤维细胞的磁聚集在液体培养基中或包埋在水凝胶中。通过光学成像表征球体的生长,同时使用ATP定量和流式细胞术评估活力。结果显示球体形成和生长成功。进一步分析表明,一方面,液体培养和基于atp的活力评估对于初始实验是可行的。另一方面,水凝胶培养和流式细胞术虽然资源和劳动密集,但提供了更可重复性和更详细的活力分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Formation and Characterization of Two Magnetic Three-Dimensional Spheroid Models of Murine Pancreatic Adenocarcinoma.

Formation and Characterization of Two Magnetic Three-Dimensional Spheroid Models of Murine Pancreatic Adenocarcinoma.

Formation and Characterization of Two Magnetic Three-Dimensional Spheroid Models of Murine Pancreatic Adenocarcinoma.

Formation and Characterization of Two Magnetic Three-Dimensional Spheroid Models of Murine Pancreatic Adenocarcinoma.

Pancreatic adenocarcinoma remains one of the deadliest cancers, with limited treatment options and high chemoresistance. Traditional 2D cell cultures fail to accurately replicate the tumor architecture. Our study introduces three-dimensional (3D) pancreatic adenocarcinoma spheroid models using magnetic aggregation of pancreatic cancer cells and immortalized fibroblasts in either liquid culture medium or embedded in hydrogels. The spheroids' growth was characterized using optical imaging, while viability was assessed using ATP quantification and flow cytometry. Results demonstrated successful spheroid formation and growth. Further analysis suggested that on one hand, culture in liquid medium and ATP-based viability assessment are practical for initial experiments. On the other hand, hydrogel culture and flow cytometry, although being more resource- and labor-intensive, provided both a more reproducible and detailed viability analysis.

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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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