Microbial drug resistance最新文献

{"title":"<i>In-Vitro</i> Activity of Dimercaptosuccinic Acid in Combination with Carbapenems Against Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>.","authors":"Maxime Bouvier, Samanta Freire, Jacqueline Findlay, Patrice Nordmann","doi":"10.1089/mdr.2024.0104","DOIUrl":"https://doi.org/10.1089/mdr.2024.0104","url":null,"abstract":"<p><p>Carbapenenemase producers, particularly the metallo-β-lactamase (MBL) types in <i>Pseudomonas aeruginosa</i>, have emerged as an urgent threat in health care settings. MBLs require zinc at their catalytic site and can be inhibited by dimercaptosuccinic acid (DMSA), a metal chelator known for the treatment of lead and mercury intoxication. Isogenic strains of wild-type and OprD-deleted <i>P. aeruginosa</i> PA14, were constructed, producing the MBLs VIM-2, NDM-1, SPM-1, IMP-1, and AIM-1, or the non-MBL carbapenemases, GES-5 and KPC-2. In addition, 59 previously characterized clinical isolates of <i>P. aeruginosa</i> producing different ß-lactamases (including carbapenemases), and with known outer-membrane porin OprD status, were utilized. Minimal inhibitory concentrations values of imipenem and meropenem, and DMSA combinations were determined, and time-kill assays were performed with PA14 expressing VIM-2. Results indicated a significant additive effect of DMSA (most effective at 3 mM) and carbapenems in recombinant and clinical strains of <i>P. aeruginosa</i> expressing MBLs, in particular against VIM producers, which are the most prevalent carbapenemases in <i>P. aeruginosa</i>. This effect was best evidenced with meropenem and in strains without OprD modification. DMSA shows promising efficacy, particularly in combination therapy with meropenem, for treating infections caused by MBL-producing <i>P. aeruginosa</i>.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Selective Culture Medium for Screening Aztreonam-Avibactam Resistance in Enterobacterales and <i>Pseudomonas aeruginosa</i>.","authors":"Soraya Herrera-Espejo, Maxime Bouvier, Elisa Cordero, Laurent Poirel, María Eugenia Pachón-Ibáñez, Patrice Nordmann","doi":"10.1089/mdr.2024.0150","DOIUrl":"https://doi.org/10.1089/mdr.2024.0150","url":null,"abstract":"<p><p>Aztreonam/avibactam (ATM/AVI) has been recently approved drug for clinical use in the European Union. The aim of this study was to develop and evaluate a novel selective medium for the isolation of ATM/AVI-resistant strains (Super ATM/AVI selective medium) to help to control their spread. Minimum inhibitory concentrations of ATM/AVI were determined using the broth microdilution method for 77 Gram-negative isolates, including 62 Enterobacterales and 15 <i>Pseudomonas aeruginosa</i>. The Super ATM/AVI selective medium was elaborated using optimal final concentrations of ATM at 5 mg/L and AVI at 4 mg/L, being supplemented with amphotericin B and vancomycin to prevent growth of yeasts and Gram-positive bacteria and with ZnSO₄ to optimize the expression of metallo-β-lactamase producers. Super ATM/AVI showed high sensitivity (94.6%) and specificity (100%) at a detection limit of 10³ CFU/mL.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the Resistome and Mobiolme of an Avian-Associated <i>Enterococus faecalis</i> ST249 Clone that Acquired Vancomycin Resistance Isolated from Neutropenic Patient in Tunisia.","authors":"Anis Raddaoui, Yosra Chebbi, Siwar Frigui, Rim Werheni Ammeri, Nour Ben Abdejlil, Mohamed Salah Abbassi, Wafa Achour","doi":"10.1089/mdr.2024.0144","DOIUrl":"https://doi.org/10.1089/mdr.2024.0144","url":null,"abstract":"<p><p>This study aimed to characterize the first vancomycin-resistant <i>Enterococcus faecalis</i> (VREfs) isolate from patient with neutropenic in Tunisia by whole-genome sequencing (WGS). This strain was detected from routine rectal swab from an 8-year-old child with bone marrow aplasia, residing in a rural area, on September 20, 2021. The strain was isolated after 12 days of hospitalization at the National Bone Marrow Transplant Center. Minimum Inhibitory Concentrations of vancomycin and teicoplanin were >256 and 16 mg/L, respectively. WGS revealed that the strain belonged to the ST249 clone, exclusively reported in avian (poultry and ducks) vancomycin-susceptible <i>E. faecalis</i> isolates in six studies from four countries, primarily Denmark. The <i>vanA</i> gene was carried by the Tn<i>1546</i> transposon mobilized by a pTW9-like plasmid. The <i>ardA</i> gene, a CRISPR-Cas system neutralization factor, was detected in this strain. In summary, this is the first report of avian-associated <i>E. faecalis</i> ST249 in clinical samples. Initially vancomycin susceptible, the strain acquired a pTW9-like plasmid carrying the classical <i>vanA</i>-Tn<i>1546</i> transposon. This acquisition was facilitated by the sex pheromone-response mechanisms and the <i>ard</i>A gene and CRISPR-Cas system neutralization.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spreading Ability of <i>Tet(X)</i>-Harboring Plasmid and Effect of Tetracyclines as a Selective Pressure.","authors":"Akira Fukuda, Yuta Kozaki, Cemil Kürekci, Yasuhiko Suzuki, Chie Nakajima, Masaru Usui","doi":"10.1089/mdr.2024.0115","DOIUrl":"https://doi.org/10.1089/mdr.2024.0115","url":null,"abstract":"<p><p>Tigecycline is a last-resort antimicrobial in humans. Tetracyclines are the most widely used antimicrobials in livestock. Mobile tigecycline resistance genes [<i>tet(X)</i>] are disseminated worldwide, and tetracycline use may have promoted the selection of <i>tet(X)</i> genes. Thus, the selective pressure on <i>tet(X)</i> genes and their plasmids in livestock must be elucidated. We performed a retrospective study to clarify the prevalence of tigecycline-resistant <i>Escherichia coli</i> from pigs in Thailand. Screening for tigecycline resistance was performed on 107 <i>E. coli</i> strains from 25 samples, and <i>tet(X)</i>-carrying plasmids were characterized. <i>tet(X)</i> genes were cloned and expressed in <i>E. coli</i>. Bacterial growth rate in the presence of tetracycline as a result of the presence of <i>tet(X)</i> genes was also evaluated. Thirty-two <i>tet(X4)</i>-harboring tigecycline-resistant <i>E. coli</i> strains were detected in 10/25 samples (40%). The <i>tet(X4)</i> genes were carried on various Inc-type plasmids and flanked by IS<i>CR2</i>. The <i>tet(X)</i>-carrying plasmids were transferred to <i>E. coli</i> and <i>Klebsiella pneumoniae</i>. Acquisition of <i>tet(X)</i> genes and their plasmids improved bacterial growth in the presence of tetracycline. In summary, tetracycline use exerts selective pressure on <i>tet(X)</i> genes and their various backbone plasmids; therefore, a reduced amount of tetracycline use is important to limit the spreading of <i>tet(X)</i> genes.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of <i>Salmonella enterica</i> Serovar Heidelberg Producing OXA 48 Carbapenemase in Eastern Algeria.","authors":"Selma Bouheraoua, Abdesselam Lezzar, Farida Assaous, Chafia Bentchouala, Sadjia Mahrane, Kaddour Benlabed, Hassiba Tali Maamar","doi":"10.1089/mdr.2023.0287","DOIUrl":"https://doi.org/10.1089/mdr.2023.0287","url":null,"abstract":"<p><p><i>Salmonella</i> infections have become increasingly resistant to antibiotics, including fluoroquinolones, third-generation cephalosporins (C3G), and even carbapenems. This report describes the emergence of a strain of <i>Salmonella enterica</i> serovar Heidelberg that produces the carbapenemase OXA 48. The strain was isolated from a stool sample taken from a newborn. Antimicrobial susceptibility testing was carried out following the recommendations of the Clinical and Laboratory Standard Institute. Whole genome sequencing was performed on MiSeq Illumina™. The strain was resistant to ertapenem (minimal inhibitory concentration [MIC] = 12 µg/mL), intermediate to imipenem (MIC = 1.5 µg/mL), resistant to nalidixic acid, and intermediate to fluoroquinolones but was susceptible to C3G, cotrimoxazole, chloramphenicol, and colistin (MIC = 0.064 µg/mL). The strain was identified as ST-15. The strain of <i>Salmonella</i> Heidelberg ST-15 was found to have antimicrobial resistance genes, specifically <i>blaOXA-48</i>, aac(6')-Iaa and <i>fosA7</i>, which mediate resistance to carbapenems, aminoglycosides and fosfomycin, respectively. Additionally, mutations were detected in the <i>gyrA</i>, <i>parC.</i> Three plasmid replicon type IncL, IncX1, and Col156 have been identified. The strain has the potential to cause an epidemic. The genomic analysis of the strain allowed us to understand the mechanisms of resistance. Preventing the spread of <i>Salmonella</i> carbapenemase-producing strains is crucial, particularly in hospital settings. Epidemiological measures are necessary to achieve this goal.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Carbapenemase-Producing <i>Pseudomonas aeruginosa</i> at Secondary Care Hospital in Bolu, Turkey.","authors":"Zuhal Kalaycı Çekin, Elif Seren Tanrıverdi, Barış Otlu","doi":"10.1089/mdr.2024.0067","DOIUrl":"https://doi.org/10.1089/mdr.2024.0067","url":null,"abstract":"<p><p>The global increase in carbapenem resistance poses a significant public health threat due to the potential emergence of multidrug-resistant pathogens and limited treatment options. To learn more about this issue and offer potential solutions, we conducted a study of carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) infections in a secondary care hospital setting. The study utilized the carbapenem inactivation method (CIM), a leading phenotypic analysis, to determine carbapenemase activity in 63 CRPA isolates. Additionally, polymerase chain reaction (PCR) analysis was conducted to test for the presence of carbapenemase genes associated with the production or expression of various carbapenemase enzymes, including <i>bla</i>_KPC, <i>bla</i>_NDM, <i>bla</i>_VIM, <i>bla</i>_OXA-48, <i>bla</i>_IMP, and <i>bla</i>_GES. Arbitrary primed PCR (AP-PCR) was performed to assess the clonal relationship between different isolates. The isolates were also classified as either health care-associated infections or community-acquired infections, and their clonal relationship and gene positivity were evaluated. A total of 63 CRPA samples underwent evaluation, with 14 isolates determined to be carbapenemase producers via CIM tests. PCR assays revealed that 14 isolates carried carbapenemase genes, with 9 carrying <i>bla</i>_NDM, 2 carrying <i>bla</i>_GES, 2 carrying <i>bla</i>_VIM, and 1 carrying <i>bla</i>_IMP. CRPA exhibited a 22% prevalence of carbapenemase genes, of which 64% were attributed to the NDM gene responsible for multidrug resistance. AP-PCR revealed high clonal diversity among the isolates. Molecular epidemiological evaluation also showed no dominant outbreak strain among PA isolates. This study presents significant data on the prevalence and distribution of carbapenemase-producing CRPA strains isolated from secondary health care facilities. Typically, the literature focuses on resistance rates in tertiary care public hospitals. These findings may aid in understanding resistance and its mechanisms, as well as in developing effective treatment strategies and infection control measures.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":"30 11","pages":"450-457"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterobacterales Producing ESBLs and AmpC in Fresh Vegetables from Tebessa City, Algeria.","authors":"Amel Amra, Manel Debabza, Raoudha Dziri, Abdelbasset Mechai, Hadda Imene Ouzari, Naouel Klibi","doi":"10.1089/mdr.2024.0042","DOIUrl":"10.1089/mdr.2024.0042","url":null,"abstract":"<p><p>This study aimed to evaluate the contamination levels of fresh products by ESBLs-producing Enterobacterales (ESBLs-E) or AmpC-producing Enterobacterales and characterize ESBLs genes. A total of 132 samples (67 vegetables and 65 fruits) were collected from markets in Tebessa, eastern Algeria. Among the samples, 16 third-generation cephalosporin-resistant Enterobacterales isolates were identified with a prevalence of 19.40% in vegetable samples, while there was no positive finding in fruit samples. Isolates showed resistance to most β-lactams, and all of them displayed multidrug resistance. Phenotypic tests for ESBLs detection, using double-disk synergy test and double-disk test were positive for 14 strains, including <i>Klebsiella pneumoniae</i> (<i>n</i> = 5), <i>Klebsiella oxytoca</i> (<i>n</i> = 4), <i>Klebsiella terrigena</i> (<i>n</i> = 2), <i>Kluyvera</i> spp. (<i>n</i> = 2), and <i>Enterobacter cloacae</i> (<i>n</i> = 1). Two AmpC-producing strains (<i>Citrobacter freundii</i> and <i>E. cloacae</i>) were identified through the AmpC disk test. Contamination rates of vegetables by ESBLs-E and AmpC-producing Enterobacterales were 19.40% and 2.98%, respectively. PCR results showed the presence of at least one ESBL gene in seven selected strains, with the dominance of <i>bla</i>_CTX-M gene. Notably, <i>K. pneumoniae</i> strains showed the co-occurrence of two or three genes. Sequencing identified uncommon variants of ESBLs genes for the first time in Algeria, including <i>bla</i>_CTX-M-79 (2/7), <i>bla</i>_CTX-M-107 (2/7), <i>bla</i>_CTX-M-117 (2/7), <i>bla</i>_TEM-112 (1/7), <i>bla</i>_TEM-125 (2/7), <i>bla</i>_TEM-194 (1/7), and <i>bla</i>_SHV-176 (3/7).</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"458-467"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avian Pathogenic <i>Escherichia coli</i> Isolated in Poultry Farms in Bangladesh that Use Antibiotics Extensively.","authors":"Badrul Hasan, Md Zulfekar Ali, Grant Rawlin","doi":"10.1089/mdr.2024.0005","DOIUrl":"10.1089/mdr.2024.0005","url":null,"abstract":"<p><p>Colibacillosis caused by avian pathogenic <i>Escherichia coli</i> (APEC) is causing economic losses to the global poultry industry. Increased prevalence of antibiotic resistance in APEC is the leading cause for increased indiscriminate use of various antimicrobial compounds in farms. The study aimed to investigate the presence of phenotypic and genotypic markers for antibiotic resistance, metals, and biocides in APEC from Bangladeshi poultry and details about the antimicrobials used in poultry farms. In total, 55 APEC were isolated from hearts or liver samples of 86 sick or dead chickens using culture on agar plate and biochemical testing. APEC isolates were tested for antibiotic susceptibility to 14 antimicrobial agents according to the European Committee on Antimicrobial Susceptibility Testing. A series of PCRs was performed to screen the presence of genes for quinolones, colistin, aminoglycosides, ESBL, metals, and biocides. Detailed information regarding antibiotic use was collected from farmers during clinical investigations. Resistance was found to 10 antibiotics and prevalence was as follows: ampicillin (86%), ciprofloxacin (86%), trimethoprim-sulphamethoxazole (73%), chloramphenicol (33%), mecillinam (13%), gentamicin (11%), cefoxitin (11%), cefotaxime (9%), tigecycline (2%), and nitrofurantoin (2%). The most common multiresistance phenotype was CIP-AMP-SXT, and 35% of isolates were multidrug resistant. Genotypic analysis confirmed the presence of quinolone resistance genes [<i>qnrS1</i> and <i>aac-(6')-lb-cr</i>], silver-resistant genes (<i>silE</i>), and mercury-resistant genes (<i>merA</i>) but not others. In total, 88% farmers were using different antimicrobial compounds, and, of them, 56% were using antimicrobials without prescriptions from veterinarians. Ciprofloxacin was most extensively used followed by oxytetracycline. Critically important antibiotics like ciprofloxacin, colistin, and gentamicin are extensively used in the farms. This study confirmed the presence of antibiotics, metals, and biocide-resistant APEC in poultry farms in Bangladesh. Increased resistance to quinolones is a serious ongoing problem. The indiscriminate use of antibiotics in poultry farms is alarming and should be stopped.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"468-475"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Pharmacokinetics and Bone Penetration of Novel Broad-Spectrum Anti-MRSA Agent Levonadifloxacin in Wistar Rats.","authors":"Rajesh Chavan, Vineet Zope, Avinash Karade, Manohar Nandanwar, Sachin Bhagwat","doi":"10.1089/mdr.2024.0118","DOIUrl":"10.1089/mdr.2024.0118","url":null,"abstract":"<p><p><b><i>Objectives:</i></b> Levonadifloxacin (IV) and alalevonadifloxacin (oral) are novel broad-spectrum anti-methicillin-resistant <i>Staphylococcus aureus</i> (<i>S. aureus</i>) agents based on novel benzoquinolizine core. Both are recently approved in India for the treatment of acute bacterial skin and skin structure infections, including diabetic foot infections and concurrent bacteremia. The present investigation reports the findings from preclinical pharmacokinetic (PK) studies that support the development of levonadifloxacin as a treatment option for bone and joint infections (BJIs). <b><i>Methods:</i></b> PK profiles of levonadifloxacin were obtained in serum, and/or various anatomical segments of femoral bone such as whole bone (WB), hard bone (HB), and bone marrow (BM) following subcutaneous administration of levonadifloxacin single doses at 50, 100, 200, and 400 mg/kg, as well as multiple doses at 200 mg/kg (BID (two times a day), 6 hours apart) for 5 days in Wistar rats. <b><i>Results:</i></b> The distribution of levonadifloxacin in bone was rapid, and the extent of distribution (B/S ratio; bone-to-serum area under the concentration-time curve ratio) was nearly comparable across bone segments. In single-dosage PK studies, the mean B/S ratio in WB, HB, and BM was 0.40, 0.33, and 0.34, respectively; however, in 5 days' repeated dose studies, it increased to 1.01, 1.14, and 0.61, respectively. <b><i>Conclusions:</i></b> On the basis of bone PK data in Wistar rat and ever-growing clinical experience in terms of safety and efficacy, levonadifloxacin has the potential to offer a well-differentiated therapy for the treatment of BJIs.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"443-449"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Colistin HiMIC Plate Kit for Colistin Susceptibility Testing of <i>Klebsiella pneumoniae</i>.","authors":"Lucija Kanižaj, Ivana Mareković, Tomislav Kuliš, Ana Budimir","doi":"10.1089/mdr.2024.0141","DOIUrl":"10.1089/mdr.2024.0141","url":null,"abstract":"<p><p>Colistin HiMIC Plate Kit (HiMedia Laboratories), a new commercial broth microdilution (BMD) test for colistin susceptibility testing was evaluated. BMD according to ISO standard 20776-1 (2019) with two-fold dilutions from 128 to 0.125 mg/L was used as a reference method. The colistin reference MICs (minimal inhibitory concentration) ranged from 0,25 to 128 mg/L with 15 (20.5%; 15/73) isolates having colistin reference MICs close to the current EUCAST breakpoint (MICs of 2, 4, and 8 mg/L). The study assessed the compliance of a commercial kit with the CLSI criteria, including categorical agreement (CA) and essential agreement (EA ≥90%), very major error (VME rate) <3%, and major error (ME) rate <3%. On 73 carbapenemase-producing <i>Klebsiella pneumoniae</i> isolates Colistin HiMIC^TM Plate Kit showed CA and EA of 100% (73/73; 95% CI: 0.97-1.00) and 82.2% (60/73; 95% CI: 0.72-0.90), respectively. No ME (false-resistant results) and VME (false-susceptible results) were detected. Kit showed acceptable CA, ME, and VME error parameters, whereas the EA did not meet the ≥90% threshold. Laboratories must check for possible limitations of commercial kits before they can be used for colistin susceptibility testing.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"476-479"},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142350225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}