Microbial drug resistance最新文献

Influential Factors in the Treatment of Pseudomonas aeruginosa Infections at a Tertiary Hospital in Vietnam.

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-06 DOI: 10.1089/mdr.2024.0191

Tiep Khac Nguyen, Ngoc Khanh Le, Pham Hong Nhung, Thao Thi Huong Bui, Gang Wang, Françoise Van Bambeke, Phung Thanh Huong

{"title":"Influential Factors in the Treatment of Pseudomonas aeruginosa Infections at a Tertiary Hospital in Vietnam.","authors":"Tiep Khac Nguyen, Ngoc Khanh Le, Pham Hong Nhung, Thao Thi Huong Bui, Gang Wang, Françoise Van Bambeke, Phung Thanh Huong","doi":"10.1089/mdr.2024.0191","DOIUrl":"https://doi.org/10.1089/mdr.2024.0191","url":null,"abstract":"As an opportunistic pathogen, Pseudomonas aeruginosa is often associated with severe respiratory infections. A study conducted in an ICU of a tertiary hospital in Vietnam, where infection management is relatively good, yielded only 18 clinical isolates of P. aeruginosa over 6 months. Though the number is small, treating P. aeruginosa infections is highly complicated. Out of 18 patients, 15 showed no improvement after treatment, leading to worsening conditions or death, possibly due to various factors. High rates of mechanical ventilation (83.3%) may be a contributing factor, suggesting a certain correlation between ventilation and treatment failure. The antibiotic resistance rate in these isolates is relatively high, with a multidrug-resistant rate of 44.4%, resulting in treatment failures when empirical antibiotics are used without susceptibility testing. All isolates have the ability to form biofilms. Moreover, bacteria in stationary phase or within biofilms exhibited poor responses to meropenem and amikacin (about 10% of bacteria survive after antibiotic exposure). Conversely, ciprofloxacin shows much better efficacy, indicating that fluoroquinolones should be used in combination therapy for P. aeruginosa infection to eliminate persistent cells and biofilm-embedded microorganisms, thus enhancing treatment effectiveness.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TB Anywhere, TB Everywhere. The First Case of pre-Extensively Drug Resistant Tuberculosis Treated with BPaL in Italy: Challenges and Opportunities for Transborder Collaboration.

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-12-12 DOI: 10.1089/mdr.2024.0045

Giovanni Fumagalli, Niccolò Riccardi, Gaia Catalano, Maurizio Ferrarese, Alice Claudia Repossi, Luigi R Codecasa

引用次数: 0

In-Vitro Activity of Dimercaptosuccinic Acid in Combination with Carbapenems Against Carbapenem-Resistant Pseudomonas aeruginosa. 二巯丁二酸与碳青霉烯类药物联用对耐碳青霉烯类铜绿假单胞菌的体外活性

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-11-25 DOI: 10.1089/mdr.2024.0104

Maxime Bouvier, Samanta Freire, Jacqueline Findlay, Patrice Nordmann

{"title":"In-Vitro Activity of Dimercaptosuccinic Acid in Combination with Carbapenems Against Carbapenem-Resistant Pseudomonas aeruginosa.","authors":"Maxime Bouvier, Samanta Freire, Jacqueline Findlay, Patrice Nordmann","doi":"10.1089/mdr.2024.0104","DOIUrl":"10.1089/mdr.2024.0104","url":null,"abstract":"Carbapenenemase producers, particularly the metallo-β-lactamase (MBL) types in Pseudomonas aeruginosa, have emerged as an urgent threat in health care settings. MBLs require zinc at their catalytic site and can be inhibited by dimercaptosuccinic acid (DMSA), a metal chelator known for the treatment of lead and mercury intoxication. Isogenic strains of wild-type and OprD-deleted P. aeruginosa PA14, were constructed, producing the MBLs VIM-2, NDM-1, SPM-1, IMP-1, and AIM-1, or the non-MBL carbapenemases, GES-5 and KPC-2. In addition, 59 previously characterized clinical isolates of P. aeruginosa producing different ß-lactamases (including carbapenemases), and with known outer-membrane porin OprD status, were utilized. Minimal inhibitory concentrations values of imipenem and meropenem, and DMSA combinations were determined, and time-kill assays were performed with PA14 expressing VIM-2. Results indicated a significant additive effect of DMSA (most effective at 3 mM) and carbapenems in recombinant and clinical strains of P. aeruginosa expressing MBLs, in particular against VIM producers, which are the most prevalent carbapenemases in P. aeruginosa. This effect was best evidenced with meropenem and in strains without OprD modification. DMSA shows promising efficacy, particularly in combination therapy with meropenem, for treating infections caused by MBL-producing P. aeruginosa.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"16-20"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Implications of the Skip Phenomenon in Patients with Persistent Staphylococcus aureus Bacteremia.

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-12-05 DOI: 10.1089/mdr.2024.0107

Sukbin Jang, Minji Jeon, Si-Ho Kim, Seok Jun Mun

{"title":"Clinical Implications of the Skip Phenomenon in Patients with Persistent Staphylococcus aureus Bacteremia.","authors":"Sukbin Jang, Minji Jeon, Si-Ho Kim, Seok Jun Mun","doi":"10.1089/mdr.2024.0107","DOIUrl":"10.1089/mdr.2024.0107","url":null,"abstract":"Intermittent negative blood cultures, known as the skip phenomenon (SP), frequently occur in patients with Staphylococcus aureus bacteremia (SAB), yet the clinical implications of SP in persistent SAB are not well understood. In this retrospective cohort study conducted at four university hospitals, SP was observed in 25 (11.3%) of 221 patients with persistent SAB. Infections involving methicillin-resistant S. aureus (MRSA) were more prevalent in patients with SP, who also experienced longer durations of bacteremia and delayed active antibiotic therapy compared with those without SP. The 30-day in-hospital mortality was lower in patients with SP than in those without SP (12.0% vs. 30.6%, respectively, p = 0.052). The median time from the initiation of active antibiotic therapy to the occurrence of SP was 6 days, and from SP to the last positive blood culture was 7 days. The duration of bacteremia and MRSA were independent predictors of SP. These findings suggest that SP can cause the duration of bacteremia to be underestimated by more than 1 week, indicating that confirmation of serial negative blood cultures might be necessary to reliably rule out SP in patients with prolonged MRSA bacteremia.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"21-25"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in CRKP Prevalence and Risk Factors for CRKP Hospital-Acquired Infections in Pediatric Patients Pre-, During-, and Post-COVID-19 Pandemic.

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-12-10 DOI: 10.1089/mdr.2024.0136

Chengjiao Luo, Qian Chen

{"title":"Trends in CRKP Prevalence and Risk Factors for CRKP Hospital-Acquired Infections in Pediatric Patients Pre-, During-, and Post-COVID-19 Pandemic.","authors":"Chengjiao Luo, Qian Chen","doi":"10.1089/mdr.2024.0136","DOIUrl":"10.1089/mdr.2024.0136","url":null,"abstract":"This study aims to delineate the epidemiological trends of carbapenem-resistant Klebsiella pneumoniae (CRKP) in pediatric patients before, during, and after coronavirus disease 2019 (COVID-19) pandemic and to assess the risk factors of CRKP hospital-acquired infections (CRKP-HAIs) across these three periods. We retrospectively collected the clinical data of pediatric patients diagnosed with K. pneumoniae infection at the Children's Hospital of Nanjing Medical University from January 2018 to March 2024. Carbapenemase-related genes were detected by PCR, and statistical analysis was conducted using SPSS 25.0. The current study found that modifications in the COVID-19 pandemic prevention and control measures and antibiotic therapies impact the epidemiological trends and antimicrobial resistance of CRKP. Binary logistic regression analyses revealed various independent risk factors for CRKP-HAIs before, during, and after the COVID-19 pandemic. Healthcare institutions must intensify surveillance for HAIs, continuously monitor and avoid risk factors for CRKP-HAIs, and formulate targeted preventive and control measures to effectively reduce the incidence and spread of these infections. Further, consistent surveillance of CRKP strains coproducing carbapenemase genes is crucial for mitigating the potential health risks in pediatric patients.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"1-11"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2024.

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 DOI: 10.1089/mdr.2024.85214.revack

引用次数: 0

Deciphering the Resistome and Mobiolme of an Avian-Associated Enterococus faecalis ST249 Clone that Acquired Vancomycin Resistance Isolated from Neutropenic Patient in Tunisia. 解密从突尼斯中性粒细胞减少患者身上分离出的禽相关粪肠球菌 ST249 克隆的耐药性和万古霉素耐药性。

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.1089/mdr.2024.0144

Anis Raddaoui, Yosra Chebbi, Siwar Frigui, Rim Werheni Ammeri, Nour Ben Abdejlil, Mohamed Salah Abbassi, Wafa Achour

{"title":"Deciphering the Resistome and Mobiolme of an Avian-Associated Enterococus faecalis ST249 Clone that Acquired Vancomycin Resistance Isolated from Neutropenic Patient in Tunisia.","authors":"Anis Raddaoui, Yosra Chebbi, Siwar Frigui, Rim Werheni Ammeri, Nour Ben Abdejlil, Mohamed Salah Abbassi, Wafa Achour","doi":"10.1089/mdr.2024.0144","DOIUrl":"10.1089/mdr.2024.0144","url":null,"abstract":"This study aimed to characterize the first vancomycin-resistant Enterococcus faecalis (VREfs) isolate from patient with neutropenic in Tunisia by whole-genome sequencing (WGS). This strain was detected from routine rectal swab from an 8-year-old child with bone marrow aplasia, residing in a rural area, on September 20, 2021. The strain was isolated after 12 days of hospitalization at the National Bone Marrow Transplant Center. Minimum Inhibitory Concentrations of vancomycin and teicoplanin were >256 and 16 mg/L, respectively. WGS revealed that the strain belonged to the ST249 clone, exclusively reported in avian (poultry and ducks) vancomycin-susceptible E. faecalis isolates in six studies from four countries, primarily Denmark. The vanA gene was carried by the Tn1546 transposon mobilized by a pTW9-like plasmid. The ardA gene, a CRISPR-Cas system neutralization factor, was detected in this strain. In summary, this is the first report of avian-associated E. faecalis ST249 in clinical samples. Initially vancomycin susceptible, the strain acquired a pTW9-like plasmid carrying the classical vanA-Tn1546 transposon. This acquisition was facilitated by the sex pheromone-response mechanisms and the ardA gene and CRISPR-Cas system neutralization.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"481-488"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of Salmonella enterica Serovar Heidelberg Producing OXA 48 Carbapenemase in Eastern Algeria. 阿尔及利亚东部出现产生 OXA 48 碳青霉烯酶的海德堡肠炎沙门氏菌。

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2024-12-01 Epub Date: 2024-11-06 DOI: 10.1089/mdr.2023.0287

Selma Bouheraoua, Abdesselam Lezzar, Farida Assaous, Chafia Bentchouala, Sadjia Mahrane, Kaddour Benlabed, Hassiba Tali Maamar

{"title":"Emergence of Salmonella enterica Serovar Heidelberg Producing OXA 48 Carbapenemase in Eastern Algeria.","authors":"Selma Bouheraoua, Abdesselam Lezzar, Farida Assaous, Chafia Bentchouala, Sadjia Mahrane, Kaddour Benlabed, Hassiba Tali Maamar","doi":"10.1089/mdr.2023.0287","DOIUrl":"10.1089/mdr.2023.0287","url":null,"abstract":"Salmonella infections have become increasingly resistant to antibiotics, including fluoroquinolones, third-generation cephalosporins (C3G), and even carbapenems. This report describes the emergence of a strain of Salmonella enterica serovar Heidelberg that produces the carbapenemase OXA 48. The strain was isolated from a stool sample taken from a newborn. Antimicrobial susceptibility testing was carried out following the recommendations of the Clinical and Laboratory Standard Institute. Whole genome sequencing was performed on MiSeq Illumina™. The strain was resistant to ertapenem (minimal inhibitory concentration [MIC] = 12 µg/mL), intermediate to imipenem (MIC = 1.5 µg/mL), resistant to nalidixic acid, and intermediate to fluoroquinolones but was susceptible to C3G, cotrimoxazole, chloramphenicol, and colistin (MIC = 0.064 µg/mL). The strain was identified as ST-15. The strain of Salmonella Heidelberg ST-15 was found to have antimicrobial resistance genes, specifically blaOXA-48, aac(6')-Iaa and fosA7, which mediate resistance to carbapenems, aminoglycosides and fosfomycin, respectively. Additionally, mutations were detected in the gyrA, parC. Three plasmid replicon type IncL, IncX1, and Col156 have been identified. The strain has the potential to cause an epidemic. The genomic analysis of the strain allowed us to understand the mechanisms of resistance. Preventing the spread of Salmonella carbapenemase-producing strains is crucial, particularly in hospital settings. Epidemiological measures are necessary to achieve this goal.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"509-512"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Selective Culture Medium for Screening Aztreonam-Avibactam Resistance in Enterobacterales and Pseudomonas aeruginosa. 用于筛查肠杆菌和铜绿假单胞菌对阿奇霉素-阿维菌素耐药性的选择性培养基

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2024-12-01 Epub Date: 2024-11-22 DOI: 10.1089/mdr.2024.0150

Soraya Herrera-Espejo, Maxime Bouvier, Elisa Cordero, Laurent Poirel, María Eugenia Pachón-Ibáñez, Patrice Nordmann

引用次数: 0

Spreading Ability of Tet(X)-Harboring Plasmid and Effect of Tetracyclines as a Selective Pressure. Tet(X)载体质粒的扩散能力和四环素的选择性压力效应

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.1089/mdr.2024.0115

Akira Fukuda, Yuta Kozaki, Cemil Kürekci, Yasuhiko Suzuki, Chie Nakajima, Masaru Usui

{"title":"Spreading Ability of Tet(X)-Harboring Plasmid and Effect of Tetracyclines as a Selective Pressure.","authors":"Akira Fukuda, Yuta Kozaki, Cemil Kürekci, Yasuhiko Suzuki, Chie Nakajima, Masaru Usui","doi":"10.1089/mdr.2024.0115","DOIUrl":"10.1089/mdr.2024.0115","url":null,"abstract":"Tigecycline is a last-resort antimicrobial in humans. Tetracyclines are the most widely used antimicrobials in livestock. Mobile tigecycline resistance genes [tet(X)] are disseminated worldwide, and tetracycline use may have promoted the selection of tet(X) genes. Thus, the selective pressure on tet(X) genes and their plasmids in livestock must be elucidated. We performed a retrospective study to clarify the prevalence of tigecycline-resistant Escherichia coli from pigs in Thailand. Screening for tigecycline resistance was performed on 107 E. coli strains from 25 samples, and tet(X)-carrying plasmids were characterized. tet(X) genes were cloned and expressed in E. coli. Bacterial growth rate in the presence of tetracycline as a result of the presence of tet(X) genes was also evaluated. Thirty-two tet(X4)-harboring tigecycline-resistant E. coli strains were detected in 10/25 samples (40%). The tet(X4) genes were carried on various Inc-type plasmids and flanked by ISCR2. The tet(X)-carrying plasmids were transferred to E. coli and Klebsiella pneumoniae. Acquisition of tet(X) genes and their plasmids improved bacterial growth in the presence of tetracycline. In summary, tetracycline use exerts selective pressure on tet(X) genes and their various backbone plasmids; therefore, a reduced amount of tetracycline use is important to limit the spreading of tet(X) genes.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"489-501"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0