Microbial drug resistance最新文献

{"title":"Comparison of Biological and Genomic Characteristics Between Two Non-Intestinal <i>Salmonella Enterica</i> Serovar Enteritidis Isolates from the Same Patient.","authors":"Xin Cheng, Xinxin Chen, Liyan Ma, Jingjuan Wei, Ying Xu, Yana Li, Siyu Ma, Jiaqi Li, Wei Sun","doi":"10.1089/mdr.2024.0211","DOIUrl":"https://doi.org/10.1089/mdr.2024.0211","url":null,"abstract":"<p><p>This study investigates two isolates of <i>Salmonella enterica subspecies enterica</i> serovar Enteritidis (<i>S.</i> Enteritidis), designated Sal B and Sal D, isolated from the blood and pleural fluid, respectively, of the same patient. Drug susceptibility testing revealed significant differences: Sal D exhibited greater resistance to ticarcillin/clavulanate, piperacillin/sulbactam, and ciprofloxacin compared with Sal B. Morphologically, Sal B formed rougher and drier colonies than Sal D at 37°C. Sal B demonstrated significantly stronger biofilm-forming ability and higher adhesion capacity to HaCaT cells than Sal D, whereas Sal D showed superior adaptation to acidic conditions (pH 3.0). Virulence assays indicated no significant differences between the isolates, suggesting comparable pathogenic potential. Comparative genomic analysis showed high gene content conservation but identified two nonsynonymous single-nucleotide polymorphisms (nsSNPs) and an insertion in the <i>envZ</i> and <i>siiE</i> genes. These genetic variations may account for the observed differences in drug susceptibility and biological characteristics. Collectively, these findings suggest that <i>S.</i> Enteritidis can undergo adaptive changes in response to distinct host environments, influencing drug resistance, adhesion, and acid resistance. This knowledge may inform future strategies for the treatment and prevention of <i>Salmonella</i> infections.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Artificial Intelligence in Current Fight Against Antimicrobial Resistance.","authors":"Cyrielle Codde, Jean-François Faucher, Jean-Baptiste Woillard","doi":"10.1089/mdr.2024.0241","DOIUrl":"https://doi.org/10.1089/mdr.2024.0241","url":null,"abstract":"<p><p>Antimicrobial resistance (AMR) poses a significant global health threat, with projections indicating it could surpass cancer in mortality rates by 2050 if left unaddressed. Optimizing antimicrobial dosing is critical to mitigate resistance and improve clinical outcomes. Traditional approaches, including population pharmacokinetics (PK) models and Bayesian estimation, are limited by mechanistic hypothesis requirements and complexity. Artificial intelligence (AI) and machine learning (ML) offer transformative solutions by leveraging large datasets to predict drug exposure accurately, refine sampling strategies, and enable real-time dose adjustments through therapeutic drug monitoring. This review highlights the role of ML models, in managing PK and pharmacodynamic variability across diverse patient populations. AI models often equal or outperform traditional methods in achieving therapeutic targets while minimizing toxicity, as demonstrated in some case studies involving ganciclovir, vancomycin, and daptomycin. Despite challenges such as data quality, interpretability, and integration with clinical workflows, AI's dynamic adaptability and precision underscore its potential. Future directions emphasize integrating multi-omics data, developing bedside decision-support tools, and expanding AI applications to broader drug categories and populations. Continued research and clinical validation are essential to harness AI's full potential in advancing precision medicine and combating AMR effectively.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Antibiofilm Effects of Antibiotics on <i>Staphylococcus</i> Species from Pediatric Hematology-Oncology Patients.","authors":"Cansu Vatansever, Nilay Aksoy, Başak Adaklı Aksoy, Tunç Fışgın","doi":"10.1089/mdr.2025.0006","DOIUrl":"https://doi.org/10.1089/mdr.2025.0006","url":null,"abstract":"<p><p>Biofilms are microbial communities and occur on different medical devices such as catheters. The formation of bacterial biofilms on medical devices leads to indwelling medical device-related infections. Since biofilm bacteria are more resistant to antibiotics than planktonic bacteria, using these antibiotics in indwelling medical device-related infections causes recurrence of infections, treatment failure, and death. Minimum inhibitory concentration (MIC) is an important reference in treating acute infections caused by planktonic bacteria. However, MIC is ineffective in indwelling medical device-related infections caused by biofilm bacteria. The study aims to demonstrate the necessity and development of effective and standard methods such as minimum biofilm prevention concentration, minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration in the case of indwelling medical device-related infection. The study was conducted with 10 isolates of <i>Staphylococcus</i> species from patients who developed infections in the Pediatric Hematology-Oncology Department at Medical Park Bahcelievler Hospital. According to the study results, even if planktonic bacteria are sensitive to antibiotics, they can become resistant to this antibiotic when they are in a biofilm (<i>p</i> < 0.05, Crosstab). Also, inhibiting the growth of planktonic bacteria does not prevent biofilm formation. The study additionally revealed that inhibiting and eradicating biofilm is more difficult than preventing biofilm formation (<i>p</i> < 0.05).</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verapamil Modulates Activity of Antimicrobials Against Rapidly Growing <i>Mycobacteria</i>.","authors":"Andressa Araújo Machado do Nascimento, Carolina Trevisolli Palomo, Regiane Bertin de Lima Scodro, Katiany Rizzieri Caleffi-Ferracioli, Vera Lucia Dias Siqueira, Jean Eduardo Meneguello, Rosilene Fressatti Cardoso","doi":"10.1089/mdr.2024.0116","DOIUrl":"https://doi.org/10.1089/mdr.2024.0116","url":null,"abstract":"<p><p>Rapidly growing mycobacteria (RGM) have been causing diseases with an increasing incidence that require long and difficult treatment. In this regard, it is a priority to seek rapid and low-cost optimization of therapeutic alternatives. Thus, our objective is to explore the combined activity between verapamil (VP) and the antimicrobials clarithromycin, amikacin, and clofazimine (CFZ) against <i>Mycobacterium smegmatis</i>, <i>Mycobacterium abscessus subsp. abscessus</i>, <i>Mycobacterium abscessus subsp. massiliense</i>, <i>Mycobacterium abscessus subsp. bolletii</i>, <i>Mycobacterium chelonae</i>, and <i>Mycobacterium fortuitum</i>. According to the checkerboard assay, it was observed that the best combination was between VP and CFZ, with synergistic activity on all tested bacteria. The time-killing assay demonstrated that VP improved the killing of CFZ and extended its inhibitory activity 16 times. In this sense, VP has modulating activity with most of the tested antimicrobials, especially with CFZ, and thus may have potential activity in preventing bacterial resistance that could be pointed out as a model for synergism in attempts at screening molecules for RGM infection treatments.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":"31 5","pages":"162-167"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clonal and Plasmid-Mediated Dissemination of β-Lactamases Producing <i>Klebsiella</i> spp. Among Environment and Humans in an Intensive Vegetable Cultivation Area in Eastern China.","authors":"Kaixin Jiang, Yunting Jiang, Qian Zhao, Zhenhua Shang, Huiyun Zou, Jiliang Si, Tianle Wu, Xuewen Li","doi":"10.1089/mdr.2024.0140","DOIUrl":"https://doi.org/10.1089/mdr.2024.0140","url":null,"abstract":"<p><p><b><i>Background:</i></b> Emergence and the rising prevalence of extended-spectrum β-lactamases (ESBLs) producing multidrug-resistant <i>Klebsiella</i> spp. is a global concern. <b><i>Methods:</i></b> 391 samples were collected from environmental and people in an intensive vegetable cultivation area in eastern China in June 2019. ESBLs-producing <i>Klebsiella</i> spp. were obtained by PCR and strain identification. The resistance genotype and phenotype of the strain were determined by PCR and drug susceptibility test. The number and size of plasmids were determined by pulsed-field gel electrophoresis assays of plasmids. The plasmid of <i>bla</i>_CTX-M was determined by DNA imprinting hybridization, and the transferability of plasmid was understood by plasmid conjugation experiment. Whole-genome sequencing analysis (WGS) was used to obtain other antimicrobial resistance genes, virulence factors, mobile elements, and genetic environment. <b><i>Results:</i></b> Seventeen ESBL-producing <i>Klebsiella</i> spp. were multi-drug resistant. Sixteen ESBLs-producing <i>Klebsiella</i> spp. carried the <i>bla</i>_CTX-M, and the size of the plasmid containing the <i>bla</i>_CTX-M anged from ∼33.3 kb to ∼244.4 kb. Thirteen ESBLs-producing <i>Klebsiella</i> spp. carried the <i>bla</i>_CTX-M were successfully transferred to the recipient bacterium through plasmid mediation. Single nucleotide polymorphism analysis showed clonal transmission between river water (J4-J8) and river sediment (J9), in river water (J3) and human feces (J12). WGS showed that all <i>bla</i>_CTX-M were associated with the mobile element <i>Tn3</i> and/or <i>IS1380</i> family. All strains carried virulence factors related to adhesion, colonization, and pathogenicity. <b><i>Conclusion:</i></b> This study reminds us that antibiotic-resistant bacteria (ARB) from vegetable cultivation environments can spread to human. It is vital to enhance surveillance of the vegetable cultivation area and high vigilance for the risk of ARB movement from the vegetable plantation environment to humans.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":"31 5","pages":"133-143"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population Structure, Antimicrobial Susceptibility, and Virulence-Associated Gene Profiling of <i>Salmonella</i> from Clinical Patients in the Jiangsu Province, China, from 2015 to 2019.","authors":"Hui Cao, Kai Ma, Dongyu Zheng, Xin Qiao","doi":"10.1089/mdr.2024.0240","DOIUrl":"https://doi.org/10.1089/mdr.2024.0240","url":null,"abstract":"<p><p><i>Salmonella</i> is a foodborne zoonotic pathogen and a hazard to public health. Surveillance of the prevalence of <i>Salmonella</i> is important. This study sought to understand the population structure, antimicrobial susceptibility, and virulence-associated gene profile of 100 <i>Salmonella,</i> which were randomly selected from clinical foodborne diarrhea fecal samples during 2015 and 2019 in the Jiangsu Province, China. After whole-genome sequencing and <i>in silico</i> analysis, we found that the prevalence of clinical foodborne <i>Salmonella</i> in Jiangsu Province was periodic and that the serotypes were diverse, covering 9 serogroups and 19 serotypes. <i>S.</i> Enteritidis was the most prevalent serotype, followed by <i>S.</i> Typhimurium. A high prevalence of antimicrobial resistance was also observed in this study, nearly half (47/100) of <i>Salmonella</i> isolates were determined to be multidrug-resistant (resistant to ≥3 antimicrobial agents), the antimicrobial resistance genotype and phenotype were associated but not closely related, and antimicrobial resistance differed between the major <i>Salmonella</i> sequence types. Additionally, we found that the virulence-associated gene profile is highly concordant with the serotype. Our work shows the association among serotype, antimicrobial resistance, and virulence gene profile, demonstrating the connection between genotype and phenotype and providing epidemiological data for <i>Salmonella</i>.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":"31 5","pages":"144-153"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species Distribution and Antimicrobial Susceptibility of Diverse Strains Within <i>Burkholderia cepacia</i> Complex.","authors":"Ke Li, Huiqiong Jia, Yaxi Gu, Yanyan Xiao, Shengchao Li, Yahong Qu, Qing Yang","doi":"10.1089/mdr.2024.0220","DOIUrl":"https://doi.org/10.1089/mdr.2024.0220","url":null,"abstract":"<p><p><b><i>Purpose:</i></b> The aim of the present study was to examine the clinical distribution and antibiotic susceptibility of the <i>Burkholderia cepacia</i> complex (BCC). <b><i>Methods:</i></b> The BCC clinical strains were obtained from the First Affiliated Hospital of Zhejiang University School of Medicine in China from January 2019 to January 2024, and <i>hisA</i> gene sequencing was employed for strain identification. The <i>in vitro</i> susceptibility of various antibacterials was measured through the disk diffusion method and the broth microdilution method. <b><i>Results:</i></b> A total of 386 strains of BCC were collected. Among them, the most common strains were <i>B. cenocepacia</i> (45.9%), <i>B. multivorans</i> (45.1%), and <i>B. contaminans</i> (7.0%). <i>B. multivorans</i> was the main one in respiratory specimens, whereas <i>B. cenocepacia</i> dominated in blood specimens. <i>B. cenocepacia</i>, <i>B. multivorans</i>, and <i>B. contaminans</i> exhibited a susceptibility over 95% to meropenem and sulfamethoxazole-trimethoprim, whereas varying sensitivities were displayed to levofloxacin, ceftazidime, and minocycline (<i>p</i> < 0.05). Among tetracyclines, eravacycline exhibited the lowest minimum inhibitory concentration 90 (MIC90) values 1 µg/mL for <i>B. cenocepacia</i>, 1 µg/mL for <i>B. multivorans</i>, and 2 µg/mL for <i>B. contaminans</i>. This was followed by tigecycline (MIC90: 2 µg/mL, 2 µg/mL, and 4 µg/mL, respectively), minocycline (MIC90: 8 µg/mL, 2 µg/mL, and 8 µg/mL, respectively), and omadacycline (MIC90: 8 µg/mL, 4 µg/mL, and 16 µg/mL, respectively). Compared with the broth microdilution method, the category agreement (CA) of sulfamethoxazole-trimethoprim and ceftazidime was >95%, and the very major error was <1%, whereas the CA of minocycline and meropenem was <90%. <b><i>Conclusions:</i></b> Thus, there are differences in the <i>in vitro</i> antimicrobial susceptibility of different BCC strains, with eravacycline demonstrating lower MIC values compared with tigecycline, minocycline, and omadacycline.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":"31 5","pages":"154-161"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug Resistance and Molecular Typing Characteristics of Diarrheagenic <i>Escherichia coli</i> in Patients with Diarrhea in Chifeng, China.","authors":"Chunru Wei, Yanbo Bai, Lingxian Li, Huying Li, Peng Peng, Yunyao Zhao, Xiujun Sun, Huixia Yu","doi":"10.1089/mdr.2025.0022","DOIUrl":"https://doi.org/10.1089/mdr.2025.0022","url":null,"abstract":"<p><p>Diarrheagenic <i>Escherichia coli</i> (DEC) can cause diarrhea and other gastrointestinal diseases, leading to severe dehydration, malnutrition, and even death. The increasing drug resistance and the emergence of multidrug-resistant bacteria present significant challenges to the public health. This study employed qPCR detection, the broth microdilution method, and pulsed-field gel electrophoresis (PFGE) technology to analyze virulence gene, drug resistance, and phylogenetic relationships in DEC isolated from 1,000 stool samples of patients with diarrhea in Chifeng City from 2021 to 2024. A total of 96 strains of DEC were detected, yielding a detection rate of 9.6%. Among these, enteroaggregative <i>E. coli</i> (EAEC) comprised 72.9% (70 strains), enteropathogenic <i>E. coli</i> accounted for 26.0% (25 strains), and enterohemorrhagic <i>E. coli</i> constituted 1.1% (1 strain). The resistance rates of DEC to tetracycline (TET), ampicillin, nalidixic acid, sulfamethoxazole, and streptomycin were recorded at 60.4%, 57.3%, 51.0%, 49.0%, and 42.7%, respectively, with 51.1% of DEC strains exhibiting multidrug resistance. The PFGE banding patterns of the 96 DEC strains were highly polymorphic, with similarity coefficients ranging from 33.6% to 100.0%. Notably, a higher similarity coefficient indicated greater similarity in drug resistance phenotypes among the strains. These results indicate that the predominant type of DEC infection in patients with diarrhea in Chifeng City is EAEC, with a TET resistance rate as high as 60.4%. Furthermore, the resistance spectrum is broad, and the DNA level exhibits significant polymorphism.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Minocycline and Omadacycline Resistance Among Carbapenem-Resistant Gram-Negative Bacteria: Antimicrobial Susceptibility Testing and Molecular Characterization.","authors":"Richa Sinha, Jyoti Jyoti, Ashutosh Pathak, Chinmoy Sahu, Prabhaker Mishra, Rungmei S K Marak, Ujjala Ghoshal","doi":"10.1089/mdr.2024.0215","DOIUrl":"10.1089/mdr.2024.0215","url":null,"abstract":"<p><p>Increasing prevalence of multidrug-resistant infections has rendered the healthcare systems ineffective in managing infectious diseases. Drugs of \"last resort\" like carbapenems and polymyxins are becoming less effective in the management of antibiotic-resistant Gram-negative bacterial infections, leaving the clinicians with limited choices. Evaluation of the efficacy of other available broad-spectrum antibiotics (belonging to a different class) is warranted as a treatment alternative. The current study was undertaken to evaluate the <i>in vitro</i> antibacterial activity of minocycline and a new drug, omadacycline among carbapenem-resistant Gram-negative bacteria (GNB), isolated from clinical samples (pus and sputum) and to genotypically analyze them. A prospective cross-sectional study was conducted in a 3,200-bedded tertiary care medical center, located in Lucknow in the northern part of India. All the clinical isolates recovered from pus and sputum samples of patients admitted in intensive care units were processed according to the standard protocols. Identification and antibiotic susceptibility testing were performed, and carbapenem-resistant Gram-negative bacteria (CRGNB) showing resistance to minocycline were included in the study. Molecular screening of β-lactamase and tetracycline resistance genes was done by the conventional polymerase chain reaction method. Minimum inhibitory concentration analysis was performed using the broth microdilution technique. Among 700 CRGNB, 15.29% (<i>n</i> = 107/700) were minocycline resistant by disk diffusion method. Genetic analysis demonstrated the presence of tetracycline-resistant genes in about one-third isolates, among which the <i>tet</i>(B) gene was present in 41.12% (<i>n</i> = 44/107). Upon broth microdilution analysis, the overall minimum inhibitory concentration for minocycline was raised, wherein 4.76% (<i>n</i> = 5/107) of our clinical Gram-negative isolates were inhibited at ≤8 mg/L and 15.23% (<i>n</i> = 28/107) were inhibited at ≤16 mg/L. Omadacycline was able to inhibit 13.08% (<i>n</i> = 14/107) of the minocycline-resistant isolates at ≤4 mg/L (susceptible breakpoint for <i>Enterobacterales</i>). Based on the cut-off value proposed, 15.09% (<i>n</i> = 16/107) isolates resistant to minocycline were inhibited by omadacycline. High prevalence of multidrug-resistant bugs entails judicious use of minocycline and omadacycline. The presence of <i>tet</i> genes coexisting with <i>bla</i>_NDM and <i>bla</i>_OXA in our bacterial isolates shows that the resistance pattern in Gram-negative bacilli is regularly evolving, and a fully functional surveillance program across the health care system is needed to prevent the emergence and spread of antimicrobial resistance.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"107-112"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug-Resistant Uropathogens in Companion Animals: A Comprehensive Study from Clinical Cases and a Genomic Analysis of a CTX-M-14-Producing <i>Escherichia coli</i> ST354, a Leading Cause of Urinary Tract Infections.","authors":"Victoria T S Sakauchi, Bianca C T Silva, Amanda Haisi, João P Araújo Júnior, José S Ferreira Neto, Marcos B Heinemann, Natália C Gaeta","doi":"10.1089/mdr.2024.0208","DOIUrl":"10.1089/mdr.2024.0208","url":null,"abstract":"<p><p>Urinary tract infections (UTIs) are common in small animals, posing significant clinical challenges due to their recurrence and discomfort. This study investigated the bacterial causes and antimicrobial resistance patterns of UTIs in dogs and cats presented to an important Veterinary Teaching Hospital in São Paulo, Brazil, the largest city in Latin America. Samples were collected from 31 dogs and 9 cats via ultrasound-guided cystocentesis. Bacterial cultures were performed, species identification was accomplished with matrix-assisted laser desorption ionization-time of flight mass spectrometry, and antimicrobial susceptibility testing was done using the Kirby-Bauer method. <i>Escherichia coli</i> was the most frequently isolated pathogen, accounting for 27.9% of cases, followed by <i>Staphylococcus pseudintermedius</i>, <i>Proteus mirabilis</i>, and <i>Klebsiella pneumoniae</i>. Ampicillin resistance was observed in 70.4% of enterobacteria, with many <i>E. coli</i> strains exhibiting multidrug resistance. Whole-genome sequencing of an extended-spectrum beta-lactamase-producing uropathogenic <i>Escherichia coli</i> strain from a feline patient was performed; it was identified as ST354, a leading cause of UTIs worldwide in humans and animals, carrying the <i>bla</i>_CTX-M-14 gene and other resistance determinants. Phylogenetic analysis indicated genetic proximity between this strain and others from Brazilian poultry and environmental sources. These findings emphasize the need for antimicrobial resistance surveillance in veterinary UTIs and advocate for stricter antibiotic stewardship to inform diagnostic and therapeutic approaches within a One Health perspective.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"123-131"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}