Tao Xie, Junyong Chen, Shuangshuang Wang, Qinghuan Zhang, Weiling Xia, Shiya Su, Xuehua Lin, Fengqiu Yang, Jian Deng, Xiaobin Li, Wen Su, Wenjun Ni
{"title":"Whole-Genome Sequencing Analysis of the Multidrug-Resistant <i>Aeromonas caviae</i> Strain AC1520 Isolated from a Patient with Urinary Tract Infection.","authors":"Tao Xie, Junyong Chen, Shuangshuang Wang, Qinghuan Zhang, Weiling Xia, Shiya Su, Xuehua Lin, Fengqiu Yang, Jian Deng, Xiaobin Li, Wen Su, Wenjun Ni","doi":"10.1177/10766294251374533","DOIUrl":null,"url":null,"abstract":"<p><p><b><i>Purpose:</i></b> The Gram-negative bacterium <i>Aeromonas caviae</i> is an important opportunistic facultative anaerobic pathogen. In the present study, we aimed to elucidate the whole-genome sequence of the multidrug-resistant (MDR) <i>A. caviae</i> strain AC1520, detailing its acquired antibiotic resistance genes (ARGs) and their genetic elements. <b><i>Patients and Methods:</i></b> The <i>A. caviae</i> strain AC1520 was isolated from a urine sample taken from a patient with urinary tract infection. Whole-genome sequencing was performed following strain identification and antimicrobial susceptibility testing. Overall, we identified ARGs, integrons, insertion sequences (IS), and transposons acquired by strain AC1520, systematically analyzing the genetic elements associated with these ARGs. <b><i>Results:</i></b> The <i>A. caviae</i> strain AC1520 contained a circular chromosome and a plasmid. Multilocus sequence typing revealed that this strain belonged to ST-1056. All ARGs within this strain were distributed on the circular chromosome. We identified two MDR regions: (1) IS common region 1 (IS<i>CR1</i>) and class 1 integron (Int<i>I1</i>) elements associated with <i>aadA16</i>, <i>aac(6')-Ib-cr</i>, <i>catB3</i>, <i>qacE</i> (two copies), <i>sul1</i> (two copies), and <i>bla<sub>PER-3</sub></i>; one gene cluster structure (IS<i>6100</i>-<i>mphR(A)</i>-<i>mph(A)</i>-<i>mrx(A)</i>-IS<i>26</i>); (2) Two Int<i>I1</i> elements, linked to <i>ant(2'')-Ia</i>, <i>bla<sub>OXA-10</sub></i>, <i>aadA2b</i>, <i>aph(3'')-Ib</i>, <i>aph(6)-Id</i>, <i>ARR-2, aac(6')-Ib3, dfrA1, qacE,</i> and <i>sul1</i>. Notably, these two MDR regions were not only present in <i>A. caviae</i> but also in other bacteria, such as <i>Aeromonas hydrophila</i>, <i>Aeromonas media</i>, and <i>Edwardsiella tarda</i>. <b><i>Conclusion:</i></b> The <i>A. caviae</i> strain AC1520 with two separate MDR regions and 20 ARGs, conferring resistance to aminoglycoside, fluoroquinolone, phenicol, sulfonamide, beta-lactam, macrolide, rifampicin, and trimethoprim, was identified in a hospital in China. Mobile genetic elements including Tn<i>As1</i>, IS<i>CR1</i>, IS<i>As25</i>, IS<i>6100</i>, IS<i>26</i>, Tn<i>As3</i>, IS<i>As1</i>, and Tn<i>3</i>, were found within the MDR region, which could play important roles in the global dissemination of these resistance genes.</p>","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial drug resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/10766294251374533","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Purpose: The Gram-negative bacterium Aeromonas caviae is an important opportunistic facultative anaerobic pathogen. In the present study, we aimed to elucidate the whole-genome sequence of the multidrug-resistant (MDR) A. caviae strain AC1520, detailing its acquired antibiotic resistance genes (ARGs) and their genetic elements. Patients and Methods: The A. caviae strain AC1520 was isolated from a urine sample taken from a patient with urinary tract infection. Whole-genome sequencing was performed following strain identification and antimicrobial susceptibility testing. Overall, we identified ARGs, integrons, insertion sequences (IS), and transposons acquired by strain AC1520, systematically analyzing the genetic elements associated with these ARGs. Results: The A. caviae strain AC1520 contained a circular chromosome and a plasmid. Multilocus sequence typing revealed that this strain belonged to ST-1056. All ARGs within this strain were distributed on the circular chromosome. We identified two MDR regions: (1) IS common region 1 (ISCR1) and class 1 integron (IntI1) elements associated with aadA16, aac(6')-Ib-cr, catB3, qacE (two copies), sul1 (two copies), and blaPER-3; one gene cluster structure (IS6100-mphR(A)-mph(A)-mrx(A)-IS26); (2) Two IntI1 elements, linked to ant(2'')-Ia, blaOXA-10, aadA2b, aph(3'')-Ib, aph(6)-Id, ARR-2, aac(6')-Ib3, dfrA1, qacE, and sul1. Notably, these two MDR regions were not only present in A. caviae but also in other bacteria, such as Aeromonas hydrophila, Aeromonas media, and Edwardsiella tarda. Conclusion: The A. caviae strain AC1520 with two separate MDR regions and 20 ARGs, conferring resistance to aminoglycoside, fluoroquinolone, phenicol, sulfonamide, beta-lactam, macrolide, rifampicin, and trimethoprim, was identified in a hospital in China. Mobile genetic elements including TnAs1, ISCR1, ISAs25, IS6100, IS26, TnAs3, ISAs1, and Tn3, were found within the MDR region, which could play important roles in the global dissemination of these resistance genes.
期刊介绍：
Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports.
MDR coverage includes:
Molecular biology of resistance mechanisms
Virulence genes and disease
Molecular epidemiology
Drug design
Infection control.
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