Microbial drug resistance最新文献_第2页

Influential Factors in the Treatment of Pseudomonas aeruginosa Infections at a Tertiary Hospital in Vietnam. 越南某三级医院铜绿假单胞菌感染治疗的影响因素

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-02-01 Epub Date: 2025-01-06 DOI: 10.1089/mdr.2024.0191

Tiep Khac Nguyen, Ngoc Khanh Le, Pham Hong Nhung, Thao Thi Huong Bui, Gang Wang, Françoise Van Bambeke, Phung Thanh Huong

{"title":"Influential Factors in the Treatment of Pseudomonas aeruginosa Infections at a Tertiary Hospital in Vietnam.","authors":"Tiep Khac Nguyen, Ngoc Khanh Le, Pham Hong Nhung, Thao Thi Huong Bui, Gang Wang, Françoise Van Bambeke, Phung Thanh Huong","doi":"10.1089/mdr.2024.0191","DOIUrl":"10.1089/mdr.2024.0191","url":null,"abstract":"As an opportunistic pathogen, Pseudomonas aeruginosa is often associated with severe respiratory infections. A study conducted in an ICU of a tertiary hospital in Vietnam, where infection management is relatively good, yielded only 18 clinical isolates of P. aeruginosa over 6 months. Though the number is small, treating P. aeruginosa infections is highly complicated. Out of 18 patients, 15 showed no improvement after treatment, leading to worsening conditions or death, possibly due to various factors. High rates of mechanical ventilation (83.3%) may be a contributing factor, suggesting a certain correlation between ventilation and treatment failure. The antibiotic resistance rate in these isolates is relatively high, with a multidrug-resistant rate of 44.4%, resulting in treatment failures when empirical antibiotics are used without susceptibility testing. All isolates have the ability to form biofilms. Moreover, bacteria in stationary phase or within biofilms exhibited poor responses to meropenem and amikacin (about 10% of bacteria survive after antibiotic exposure). Conversely, ciprofloxacin shows much better efficacy, indicating that fluoroquinolones should be used in combination therapy for P. aeruginosa infection to eliminate persistent cells and biofilm-embedded microorganisms, thus enhancing treatment effectiveness.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"27-33"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emergence of Multidrug-Resistant NDM-5-Producing ST307 Klebsiella pneumoniae in Uruguay, 2023. 产ndm -5的ST307肺炎克雷伯菌2023年在乌拉圭的出现

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-02-01 Epub Date: 2025-02-05 DOI: 10.1089/mdr.2024.0065

Carmen Magallanes, Eliana Eugster, Felipe Clavijo, María Inés Siri, Jorge Cantero, Patricia Echeverría, Josefina Torello, Mercedes Castro, Carolina Márquez

{"title":"Emergence of Multidrug-Resistant NDM-5-Producing ST307 Klebsiella pneumoniae in Uruguay, 2023.","authors":"Carmen Magallanes, Eliana Eugster, Felipe Clavijo, María Inés Siri, Jorge Cantero, Patricia Echeverría, Josefina Torello, Mercedes Castro, Carolina Márquez","doi":"10.1089/mdr.2024.0065","DOIUrl":"10.1089/mdr.2024.0065","url":null,"abstract":"Carbapenem and colistin-resistant Klebsiella pneumoniae pose a significant threat to public health, particularly in intensive care units, due to high morbidity and mortality rates. This study aimed to analyze five NDM carbapenemase-producing multidrug-resistant K. pneumoniae isolates from different hospitals. Antimicrobial susceptibility testing, hypermucoviscosity analysis, biofilm production assessment, MLST, PCR, and whole-genome sequencing were conducted. All isolates harbored NDM-5 metallo-β-lactamase, belonging to MLST 307, were biofilm producers and exhibited a stop codon (Q30) along MgrB. Genomic analysis revealed multiple-replicon plasmids carrying resistance genes, notably blaNDM-5, blaCTX-M-15, rmtB, and qnrB1, with complex genetic structures encoding several mobile genetic elements, including the Tn3 family and IS26. All isolates harbored wzi173 (capsule-locus KL102), iutA (a siderophore-associated gene), and the type 3 fimbriae mrkABCDFHIJ operon. The core genome single nucleotide polymorphisms (SNPs) analysis suggests the circulation of two strains of ST307 clone (SNPs range differences 4-77). These findings highlight the potential plasticity of the high-risk ST307 clone and the urgent need for surveillance and intervention strategies to combat antimicrobial resistance. To our knowledge, this is the first report of K. pneumoniae ST307 carrying blaNDM-5 and the first description of ST307 in Uruguay. The presence of blaNDM-5 and pan-aminoglycoside resistance rmtB genes are identified for the first time in Uruguay.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"42-51"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Characterization of Colistin- and Carbapenem-Resistant Klebsiella pneumoniae: mgrB Mutations and Clonal Diversity in Pediatric Intensive Care Isolates. 耐粘菌素和碳青霉烯肺炎克雷伯菌的分子特征：儿科重症分离株mgrB突变和克隆多样性

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-02-01 Epub Date: 2025-01-24 DOI: 10.1089/mdr.2024.0110

Ayşe Hande Türk, Alper Tekeli, Duygu Öcal, Devran Gerçeker

{"title":"Molecular Characterization of Colistin- and Carbapenem-Resistant Klebsiella pneumoniae: mgrB Mutations and Clonal Diversity in Pediatric Intensive Care Isolates.","authors":"Ayşe Hande Türk, Alper Tekeli, Duygu Öcal, Devran Gerçeker","doi":"10.1089/mdr.2024.0110","DOIUrl":"10.1089/mdr.2024.0110","url":null,"abstract":"Colistin- and carbapenem-resistant Klebsiella pneumoniae (ColR CrKp) cause important health problems in pediatric intensive care units (PICUs) due to its ability to harbor multiple resistance genes and spread of high-risk clones. In this study, molecular epidemiological characteristics, transferable resistance genes, and mgrB alterations of ColR CrKp isolated from PICU were investigated. Isolates were identified by MALDI-TOF MS, and antimicrobial susceptibility tests were performed using disk diffusion method, gradient strip test, and broth microdilution method. Extended spectrum beta-lactamase, AmpC beta-lactamase, carbapenemase, 16S rRNA methyltransferase, plasmid-mediated quinolone resistance, and mcr-1 to -5 genes were investigated by polymerase chain reaction. Sanger sequencing was performed to obtain blaOXA-48-like and mgrB sequences. Pulsed-field gel electrophoresis and multilocus sequence typing were used to determine the clonal spread of the isolates. Ten ColR CrKp harboring blaOXA-48 (70%), blaOXA-232 (20%), blaCTX-M (90%), armA (20%), qnrB (20%), and qnrS (50%) were identified. No mcr genes were found, whereas mgrB mutations through modifications (A7T, C88T, and A121G) and insertion of an IS-1-like insertion sequence were determined. Isolates belonged to ST 14, ST 37, ST 101, ST 147, ST 661, ST 985, and ST 2096. It is crucial to determine the antimicrobial resistance properties and the clonal spread of the isolates to guide the treatment decisions, implement effective infection control measures, and develop novel antimicrobial strategies.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"34-41"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143033699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and Evolution of the Mobilome Associated with Antibiotic Resistance Genes in Streptococcus anginosus. 血管链球菌耐药基因移动组的多样性和进化

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-02-01 Epub Date: 2025-01-21 DOI: 10.1089/mdr.2024.0229

Yingting Wang, Taoran Liu, Yi Sida, Yuanting Zhu

{"title":"Diversity and Evolution of the Mobilome Associated with Antibiotic Resistance Genes in Streptococcus anginosus.","authors":"Yingting Wang, Taoran Liu, Yi Sida, Yuanting Zhu","doi":"10.1089/mdr.2024.0229","DOIUrl":"10.1089/mdr.2024.0229","url":null,"abstract":"Streptococcus anginosus is an important cause of pyogenic infections, bacteremia, and chronic maxillary sinusitis. Mobile genetic elements (MGEs) play a key role in lateral gene transfer, resulting in broad transfer of antibiotic resistance genes (ARGs). However, studies on ARG-associated MGEs in S. anginosus are still rare. To fill this gap, we used sequencing data from 11 clinical S. anginosus to characterize their mobilome diversity through comparative analysis. We found 47 well-characterized MGEs, including 23 putative integrative and conjugative elements (ICEs), 16 prophages/integrative mobilizable elements, and 8 composites. They were inserted into 16 positions, 4 of which were hot spots. A comprehensive analysis revealed that ARG-associated ICEs belong to four groups as follows: single serine integrases (ICESan49.2), tyrosine integrases (ICESan26.2), triple serine integrase ICEs (ICESan195.1), and a putative transposon integrase (ICESan49.1), all of which were similar to ICEs/transposons widely distributed among other streptococci. The eight composites were composed of multiple ICEs or transposons through successive accretion events (tandem or/and internal integration). In conclusion, we found that S. anginosus accumulates a variety of ARG-associated ICE/composites that may enable S. anginosus to serve as an ARG-associated MGE repository for other streptococci. The analysis of composites here provides a paradigm to further study mobilome evolution.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"52-63"},"PeriodicalIF":2.3,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TB Anywhere, TB Everywhere. The First Case of pre-Extensively Drug Resistant Tuberculosis Treated with BPaL in Italy: Challenges and Opportunities for Transborder Collaboration. 肺结核无处不在，肺结核无处不在。意大利首例BPaL治疗前广泛耐药结核病病例：跨境合作的挑战和机遇。

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-12-12 DOI: 10.1089/mdr.2024.0045

Giovanni Fumagalli, Niccolò Riccardi, Gaia Catalano, Maurizio Ferrarese, Alice Claudia Repossi, Luigi R Codecasa

引用次数: 0

In-Vitro Activity of Dimercaptosuccinic Acid in Combination with Carbapenems Against Carbapenem-Resistant Pseudomonas aeruginosa. 二巯丁二酸与碳青霉烯类药物联用对耐碳青霉烯类铜绿假单胞菌的体外活性

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-11-25 DOI: 10.1089/mdr.2024.0104

Maxime Bouvier, Samanta Freire, Jacqueline Findlay, Patrice Nordmann

{"title":"In-Vitro Activity of Dimercaptosuccinic Acid in Combination with Carbapenems Against Carbapenem-Resistant Pseudomonas aeruginosa.","authors":"Maxime Bouvier, Samanta Freire, Jacqueline Findlay, Patrice Nordmann","doi":"10.1089/mdr.2024.0104","DOIUrl":"10.1089/mdr.2024.0104","url":null,"abstract":"Carbapenenemase producers, particularly the metallo-β-lactamase (MBL) types in Pseudomonas aeruginosa, have emerged as an urgent threat in health care settings. MBLs require zinc at their catalytic site and can be inhibited by dimercaptosuccinic acid (DMSA), a metal chelator known for the treatment of lead and mercury intoxication. Isogenic strains of wild-type and OprD-deleted P. aeruginosa PA14, were constructed, producing the MBLs VIM-2, NDM-1, SPM-1, IMP-1, and AIM-1, or the non-MBL carbapenemases, GES-5 and KPC-2. In addition, 59 previously characterized clinical isolates of P. aeruginosa producing different ß-lactamases (including carbapenemases), and with known outer-membrane porin OprD status, were utilized. Minimal inhibitory concentrations values of imipenem and meropenem, and DMSA combinations were determined, and time-kill assays were performed with PA14 expressing VIM-2. Results indicated a significant additive effect of DMSA (most effective at 3 mM) and carbapenems in recombinant and clinical strains of P. aeruginosa expressing MBLs, in particular against VIM producers, which are the most prevalent carbapenemases in P. aeruginosa. This effect was best evidenced with meropenem and in strains without OprD modification. DMSA shows promising efficacy, particularly in combination therapy with meropenem, for treating infections caused by MBL-producing P. aeruginosa.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"16-20"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Implications of the Skip Phenomenon in Patients with Persistent Staphylococcus aureus Bacteremia. 持续性金黄色葡萄球菌菌血症患者跳跃现象的临床意义。

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-12-05 DOI: 10.1089/mdr.2024.0107

Sukbin Jang, Minji Jeon, Si-Ho Kim, Seok Jun Mun

{"title":"Clinical Implications of the Skip Phenomenon in Patients with Persistent Staphylococcus aureus Bacteremia.","authors":"Sukbin Jang, Minji Jeon, Si-Ho Kim, Seok Jun Mun","doi":"10.1089/mdr.2024.0107","DOIUrl":"10.1089/mdr.2024.0107","url":null,"abstract":"Intermittent negative blood cultures, known as the skip phenomenon (SP), frequently occur in patients with Staphylococcus aureus bacteremia (SAB), yet the clinical implications of SP in persistent SAB are not well understood. In this retrospective cohort study conducted at four university hospitals, SP was observed in 25 (11.3%) of 221 patients with persistent SAB. Infections involving methicillin-resistant S. aureus (MRSA) were more prevalent in patients with SP, who also experienced longer durations of bacteremia and delayed active antibiotic therapy compared with those without SP. The 30-day in-hospital mortality was lower in patients with SP than in those without SP (12.0% vs. 30.6%, respectively, p = 0.052). The median time from the initiation of active antibiotic therapy to the occurrence of SP was 6 days, and from SP to the last positive blood culture was 7 days. The duration of bacteremia and MRSA were independent predictors of SP. These findings suggest that SP can cause the duration of bacteremia to be underestimated by more than 1 week, indicating that confirmation of serial negative blood cultures might be necessary to reliably rule out SP in patients with prolonged MRSA bacteremia.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"21-25"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in CRKP Prevalence and Risk Factors for CRKP Hospital-Acquired Infections in Pediatric Patients Pre-, During-, and Post-COVID-19 Pandemic. 在covid -19大流行之前、期间和之后，儿科患者CRKP流行趋势和CRKP医院获得性感染的危险因素

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 Epub Date: 2024-12-10 DOI: 10.1089/mdr.2024.0136

Chengjiao Luo, Qian Chen

{"title":"Trends in CRKP Prevalence and Risk Factors for CRKP Hospital-Acquired Infections in Pediatric Patients Pre-, During-, and Post-COVID-19 Pandemic.","authors":"Chengjiao Luo, Qian Chen","doi":"10.1089/mdr.2024.0136","DOIUrl":"10.1089/mdr.2024.0136","url":null,"abstract":"This study aims to delineate the epidemiological trends of carbapenem-resistant Klebsiella pneumoniae (CRKP) in pediatric patients before, during, and after coronavirus disease 2019 (COVID-19) pandemic and to assess the risk factors of CRKP hospital-acquired infections (CRKP-HAIs) across these three periods. We retrospectively collected the clinical data of pediatric patients diagnosed with K. pneumoniae infection at the Children's Hospital of Nanjing Medical University from January 2018 to March 2024. Carbapenemase-related genes were detected by PCR, and statistical analysis was conducted using SPSS 25.0. The current study found that modifications in the COVID-19 pandemic prevention and control measures and antibiotic therapies impact the epidemiological trends and antimicrobial resistance of CRKP. Binary logistic regression analyses revealed various independent risk factors for CRKP-HAIs before, during, and after the COVID-19 pandemic. Healthcare institutions must intensify surveillance for HAIs, continuously monitor and avoid risk factors for CRKP-HAIs, and formulate targeted preventive and control measures to effectively reduce the incidence and spread of these infections. Further, consistent surveillance of CRKP strains coproducing carbapenemase genes is crucial for mitigating the potential health risks in pediatric patients.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"1-11"},"PeriodicalIF":2.3,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acknowledgment of Reviewers 2024. 审稿人致谢

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2025-01-01 DOI: 10.1089/mdr.2024.85214.revack

引用次数: 0

Deciphering the Resistome and Mobiolme of an Avian-Associated Enterococus faecalis ST249 Clone that Acquired Vancomycin Resistance Isolated from Neutropenic Patient in Tunisia. 解密从突尼斯中性粒细胞减少患者身上分离出的禽相关粪肠球菌 ST249 克隆的耐药性和万古霉素耐药性。

IF 2.3 4区医学

Microbial drug resistance Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.1089/mdr.2024.0144

Anis Raddaoui, Yosra Chebbi, Siwar Frigui, Rim Werheni Ammeri, Nour Ben Abdejlil, Mohamed Salah Abbassi, Wafa Achour

{"title":"Deciphering the Resistome and Mobiolme of an Avian-Associated Enterococus faecalis ST249 Clone that Acquired Vancomycin Resistance Isolated from Neutropenic Patient in Tunisia.","authors":"Anis Raddaoui, Yosra Chebbi, Siwar Frigui, Rim Werheni Ammeri, Nour Ben Abdejlil, Mohamed Salah Abbassi, Wafa Achour","doi":"10.1089/mdr.2024.0144","DOIUrl":"10.1089/mdr.2024.0144","url":null,"abstract":"This study aimed to characterize the first vancomycin-resistant Enterococcus faecalis (VREfs) isolate from patient with neutropenic in Tunisia by whole-genome sequencing (WGS). This strain was detected from routine rectal swab from an 8-year-old child with bone marrow aplasia, residing in a rural area, on September 20, 2021. The strain was isolated after 12 days of hospitalization at the National Bone Marrow Transplant Center. Minimum Inhibitory Concentrations of vancomycin and teicoplanin were >256 and 16 mg/L, respectively. WGS revealed that the strain belonged to the ST249 clone, exclusively reported in avian (poultry and ducks) vancomycin-susceptible E. faecalis isolates in six studies from four countries, primarily Denmark. The vanA gene was carried by the Tn1546 transposon mobilized by a pTW9-like plasmid. The ardA gene, a CRISPR-Cas system neutralization factor, was detected in this strain. In summary, this is the first report of avian-associated E. faecalis ST249 in clinical samples. Initially vancomycin susceptible, the strain acquired a pTW9-like plasmid carrying the classical vanA-Tn1546 transposon. This acquisition was facilitated by the sex pheromone-response mechanisms and the ardA gene and CRISPR-Cas system neutralization.","PeriodicalId":18701,"journal":{"name":"Microbial drug resistance","volume":" ","pages":"481-488"},"PeriodicalIF":2.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142687698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0