赤峰市腹泻患者致泻性大肠杆菌耐药性及分子分型特征

IF 1.9 4区 医学 Q3 INFECTIOUS DISEASES
Microbial drug resistance Pub Date : 2025-06-01 Epub Date: 2025-04-30 DOI:10.1089/mdr.2025.0022
Chunru Wei, Yanbo Bai, Lingxian Li, Huying Li, Peng Peng, Yunyao Zhao, Xiujun Sun, Huixia Yu
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引用次数: 0

摘要

致泻性大肠杆菌(DEC)可引起腹泻和其他胃肠道疾病,导致严重脱水、营养不良,甚至死亡。耐药性的增加和多重耐药细菌的出现对公共卫生构成了重大挑战。本研究采用qPCR检测、微量肉汤稀释法和脉冲场凝胶电泳(PFGE)技术,对2021 - 2024年赤峰市1000例腹泻患者粪便中分离的DEC的毒力基因、耐药性及系统发育关系进行分析。共检出DEC 96株,检出率为9.6%。其中,肠聚集性大肠杆菌(EAEC) 70株,占72.9%,肠致病性大肠杆菌25株,占26.0%,肠出血性大肠杆菌1株,占1.1%。DEC对四环素(TET)、氨苄西林、萘啶酸、磺胺甲新唑和链霉素的耐药率分别为60.4%、57.3%、51.0%、49.0%和42.7%,其中51.1%的DEC菌株出现多药耐药。96株DEC的PFGE带型具有高度多态性,相似系数在33.6% ~ 100.0%之间。值得注意的是,相似系数越高,表明菌株之间的耐药表型越相似。结果提示赤峰市腹泻患者感染DEC的主要类型为EAEC, TET耐药率高达60.4%。此外,抗性谱广泛,DNA水平表现出显著的多态性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Drug Resistance and Molecular Typing Characteristics of Diarrheagenic Escherichia coli in Patients with Diarrhea in Chifeng, China.

Diarrheagenic Escherichia coli (DEC) can cause diarrhea and other gastrointestinal diseases, leading to severe dehydration, malnutrition, and even death. The increasing drug resistance and the emergence of multidrug-resistant bacteria present significant challenges to the public health. This study employed qPCR detection, the broth microdilution method, and pulsed-field gel electrophoresis (PFGE) technology to analyze virulence gene, drug resistance, and phylogenetic relationships in DEC isolated from 1,000 stool samples of patients with diarrhea in Chifeng City from 2021 to 2024. A total of 96 strains of DEC were detected, yielding a detection rate of 9.6%. Among these, enteroaggregative E. coli (EAEC) comprised 72.9% (70 strains), enteropathogenic E. coli accounted for 26.0% (25 strains), and enterohemorrhagic E. coli constituted 1.1% (1 strain). The resistance rates of DEC to tetracycline (TET), ampicillin, nalidixic acid, sulfamethoxazole, and streptomycin were recorded at 60.4%, 57.3%, 51.0%, 49.0%, and 42.7%, respectively, with 51.1% of DEC strains exhibiting multidrug resistance. The PFGE banding patterns of the 96 DEC strains were highly polymorphic, with similarity coefficients ranging from 33.6% to 100.0%. Notably, a higher similarity coefficient indicated greater similarity in drug resistance phenotypes among the strains. These results indicate that the predominant type of DEC infection in patients with diarrhea in Chifeng City is EAEC, with a TET resistance rate as high as 60.4%. Furthermore, the resistance spectrum is broad, and the DNA level exhibits significant polymorphism.

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来源期刊
Microbial drug resistance
Microbial drug resistance 医学-传染病学
CiteScore
6.00
自引率
3.80%
发文量
118
审稿时长
6-12 weeks
期刊介绍: Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.
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