尿路致病性大肠杆菌gyrA和parC基因突变与氟喹诺酮类药物耐药性关系的研究

IF 1.9 4区 医学 Q3 INFECTIOUS DISEASES
Erfan Ghaffari Lashkenari, Maryam Sadat Mir, Mohsen Mohammadi, Kasra Javadi, Mehrdad Halaji
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引用次数: 0

摘要

简介:大肠杆菌,特别是尿路致病性大肠杆菌(UPEC)对氟喹诺酮类药物的耐药性在世界范围内日益受到关注。本研究调查了伊朗尿液中UPEC分离株gyrA和parC基因突变与氟喹诺酮类药物耐药性之间的关系。材料与方法:共收集150株UPEC菌株,筛选出12株环丙沙星耐药菌株进行分子分析。采用纸片扩散法进行药敏试验,微肉汤稀释法测定环丙沙星的最低抑菌浓度(mic)。采用聚合酶链反应和测序技术检测gyrA和parC的喹诺酮类耐药决定区(QRDRs)突变。结果:所有分离株MIC值均为bbbb4,对环丙沙星、诺氟沙星、氧氟沙星、萘啶酸等4种氟喹诺酮类药物及喹诺酮类药物均耐药。所有的分离株在两个基因中都有突变。gyrA最常见的突变是Ser-83→Leu和Asp-87→Asn,在100%的分离株中发现。同样,parC的突变包括Ser-80→Ile(83.3%)和Glu-84→Val(58.3%),普遍存在。在两个基因中观察到额外的核苷酸替换。这些突变可能导致在分离株中观察到的高水平氟喹诺酮类药物耐药性。结论:本研究结果证实,gyrA和parC基因突变主要驱动UPEC分离株的氟喹诺酮类药物耐药性。qrdr中存在的特定变化显著降低了细菌对氟喹诺酮类药物的敏感性,从而导致耐药菌株的持续存在和传播。识别这些突变提供了对耐药性机制的关键见解,这有助于制定更有效的抗菌治疗策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating the Relationship Between Mutations in gyrA and parC Genes and Resistance to Fluoroquinolones in Uropathogenic Escherichia coli Isolates.

Introduction: Fluoroquinolone resistance in Escherichia coli, particularly uropathogenic E. coli (UPEC), is a growing concern worldwide. This study investigates the association between mutations in the gyrA and parC genes and fluoroquinolone resistance in UPEC isolates from Urine samples in Iran. Materials and Methods: In total, 150 UPEC isolates were collected, and then, 12 ciprofloxacin-resistant isolates were selected for molecular analysis. Antimicrobial susceptibility testing was performed using the disk diffusion method, and minimum inhibitory concentrations (MICs) of ciprofloxacin were determined by microbroth dilution. Polymerase chain reaction and sequencing were used to detect mutations in the quinolone resistance-determining regions (QRDRs) of gyrA and parC. Results: All isolates had MIC >4 and were resistant to all four fluoroquinolones and quinolones tested, including ciprofloxacin, norfloxacin, ofloxacin, and nalidixic acid. All isolates harbored mutations in both genes. The most frequent mutations in gyrA were Ser-83→Leu and Asp-87→Asn, found in 100% of isolates. Similarly, mutations in parC, including Ser-80→Ile (83.3%) and Glu-84→Val (58.3%), were prevalent. Additional nucleotide substitutions in both genes were observed. These mutations likely contribute to the high-level fluoroquinolone resistance observed in the isolates. Conclusions: The results of this study confirm that mutations in the gyrA and parC genes primarily drive fluoroquinolone resistance in UPEC isolates. The presence of specific alterations within the QRDRs significantly reduces bacterial susceptibility to fluoroquinolones, contributing to the persistence and spread of resistant strains. Identifying these mutations provides critical insights into resistance mechanisms, which can aid in developing more effective antimicrobial therapy strategies.

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来源期刊
Microbial drug resistance
Microbial drug resistance 医学-传染病学
CiteScore
6.00
自引率
3.80%
发文量
118
审稿时长
6-12 weeks
期刊介绍: Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.
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