Chinese Ophthalmic Research最新文献_第4页

Construction of human angiopoietin-1 fukaryotic expression vecter and its expression in human umbilical mesenchymal stem cells 人血管生成素-1核表达载体的构建及其在人脐带间充质干细胞中的表达

Chinese Ophthalmic Research Pub Date : 2010-09-10 DOI: 10.3969/J.ISSN.1003-0808.2010.09.014

萧鸿, 刘帅, 李群秀, 张克剑, 侯阳, 宋慧娟, 刘学政

{"title":"Construction of human angiopoietin-1 fukaryotic expression vecter and its expression in human umbilical mesenchymal stem cells","authors":"萧鸿, 刘帅, 李群秀, 张克剑, 侯阳, 宋慧娟, 刘学政","doi":"10.3969/J.ISSN.1003-0808.2010.09.014","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.09.014","url":null,"abstract":"目的构建人血管生成素-1(Ang-1)基因的真核表达载体,并检测其在人脐带间充质干细胞(UC-MSCs)中的表达,为进一步研究Ang-1防治糖尿病视网膜病变(DR)的血管渗漏提供实验依据.方法提取人胎盘组织中的总RNA,用逆转录-聚合酶链反应(RT-PCR)特异性扩增Ang-1编码区序列,亚克隆到真核表达载体pEGFP-N1.将pEGFP-N1-Ang-1导入UC-MSCs,然后应用含质量分数10%胎牛血清的DMEM培养基对人UC-MSCs进行培养,荧光显微镜下观察Ang-1在人UC-MSCs中的表达,Western blot法检测重组体的表达.结果 RT-PCR法成功克隆人Ang-1基因编码区序列,纯度为1.82;PCR扩增的片段长度为1.5 kb,与预期片段大小相近.双酶切鉴定法可识别重组pEGFP-N1-Ang-1转染质粒,目的基因与预期基因序列的同源性为100%.人UC-MSCs转染的pEGFP-N1/Ang-1质粒能够表达Ang-1蛋白,呈现绿色荧光.转染pEGFP-N1的人UC-MSCs和对照组人UC-MSCs均未见相应的Ang-1蛋白表达.结论成功地克隆出人的Ang-1基因并构建了真核表达载体pEGFP-N1-Ang-1,为将Ang-1基因转染至人UC-MSCs治疗DR奠定了基础。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125552722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical findings of central retinal vein occlusion in population aged 40 years or less 40岁以下人群视网膜中央静脉阻塞的临床表现

Chinese Ophthalmic Research Pub Date : 2010-09-10 DOI: 10.3969/J.ISSN.1003-0808.2010.09.024

华文, 李学东, 刘颖

{"title":"Clinical findings of central retinal vein occlusion in population aged 40 years or less","authors":"华文, 李学东, 刘颖","doi":"10.3969/J.ISSN.1003-0808.2010.09.024","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.09.024","url":null,"abstract":"目的研究≤40岁视网膜中央静脉阻塞(CRVO)患者的临床表现及预后.方法对≤40岁的CRVO患者15例15眼的临床特征进行观察分析,对患眼治疗前后的最佳矫正视力和荧光素眼底血管造影(FFA)不同血管充盈期的臂-视网膜循环时间进行比较,此外对患者的临床表现,包括全身病史和相关的实验室检查进行分析.结果 ≤40岁的患者发生CRVO具有起病急、视力急剧下降、视网膜出血明显、治疗后患者视力提高显著的特点.患者治疗2个月后对数视力较治疗前明显改善(4.97±0.16 vs 3.56±0.14),差异有统计学意义(t=24.912,P＜0.01).FFA检查显示,动静脉期、静脉期以及静脉晚期的臂-视网膜循环时间治疗前分别为(21.68±2.97)s、(25.12±2.81)s和(28.13±4.37)s,治疗后分别为(13.51±1.87)s、(14.91±2.55)s和(19.10±3.02)s,治疗后较治疗前明显缩短,差异均有统计学意义(t=13.612、t=18.939、t=9.699,P＜0.01).≤40岁的CRVO患者的原发病包括高血压、糖尿病,精神紧张是主要的发病诱因.无严重并发症.结论 ≤40岁的CRVO患者挽救视力的关键在于早期发现和及时治疗。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114960071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of curcumin on sodium selenite-induced cataract in vitro 姜黄素对亚硒酸钠致白内障的体外治疗作用

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.019

杜玲玲, 高维奇, 冯万国, 马瑛娜

{"title":"Effects of curcumin on sodium selenite-induced cataract in vitro","authors":"杜玲玲, 高维奇, 冯万国, 马瑛娜","doi":"10.3969/J.ISSN.1003-0808.2010.08.019","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.08.019","url":null,"abstract":"目的观察姜黄素对亚硒酸钠诱导的体外培养兔晶状体混浊的影响,探讨姜黄素对抗晶状体氧化损伤的效果.方法将体外培养的40只正常家兔的晶状体分为5组:空白对照组、亚硒酸钠组(30μmol/L)、姜黄素A组(30mg/L)、姜黄素B组(50mg/L)及卡林-U组(质量分数1%).分别于培养后24、48、72、96h观察各组晶状体的透明度,同等条件下用数码相机拍照记录各组晶状体的形态,用分光光度计测定晶状体组织中的谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)的变化.结果在48h时,不同质量分数姜黄素组与空白对照组比较,不同分级的晶状体混浊的眼数差异无统计学意义(H=1.464,P＞0.05),姜黄素组与卡林-U组及亚硒酸钠组比较,不同分级的晶状体混浊眼数的差异有统计学意义(H=10.607,P＜0.01);72h时,不同质量分数姜黄素组与空白对照组比较,不同分级的晶状体混浊的眼数差异无统计学意义(H=3.288,P＞0.05);姜黄素组与卡林-U组及亚硒酸钠组比较,不同分级的晶状体混浊眼数的差异有统计学意义(H=8.258,P＜0.05).晶状体培养后96h,不同质量分数姜黄素组与空白对照组比较,不同分级的晶状体混浊眼数的差异有统计学意义(U=7.102,P＜0.05);不同质量分数姜黄素组与卡林-U组及亚硒酸钠组比较,不同分级的晶状体混浊眼数的差异有统计学意义(H=9.699,P＜0.05).与空白对照组比较,亚硒酸钠组GSH-Px、SOD活性明显降低,MDA浓度明显升高.不同质量分数姜黄素组和卡林-U组的GSH-Px、SOD活性高于亚硒酸钠组,低于空白对照组,MDA浓度介于空白对照组与亚硒酸钠组之间,卡林-U组的GSH-Px、SOD活性低于姜黄素组,MDA浓度高于姜黄素组.结论姜黄素及卡林-U均具有抗氧化损伤作用,均可推迟亚硒酸钠诱导的体外培养兔晶状体混浊的发生时间,姜黄素的作用强于卡林-U,但姜黄素与卡林-U最终均不能抑制白内障的发生。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124423270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of sodium hyaluronate on dry eye following laser in situ keratomileiusis 透明质酸钠治疗激光原位角膜磨圆术后干眼症的疗效

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.024

翟长斌, 周跃华, 张晶

{"title":"Effectiveness of sodium hyaluronate on dry eye following laser in situ keratomileiusis","authors":"翟长斌, 周跃华, 张晶","doi":"10.3969/J.ISSN.1003-0808.2010.08.024","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.08.024","url":null,"abstract":"目的评价质量分数0.1%透明质酸钠对准分子激光角膜原位磨镶术(LASIK)后干眼症患者的治疗作用.方法选取LASIK术后1个月诊断为干眼症的患者21例42眼,应用0.1%透明质酸钠滴眼液局部点眼法连续治疗30d.0.1%透明质酸钠滴眼液的治疗效果评价指标包括患者主觉症状的临床评分变化和客观指标的改善情况,如泪膜破裂时间(BUT)、角膜荧光素着色评分、SchirmerⅠ试验.对0.1%透明质酸钠滴眼液治疗前和治疗后1、2、4周上述指标的变化进行比较.结果所有患者均按要求完成治疗和随访.0.1%透明质酸钠治疗4周,患者主觉症状评分较用药前明显下降,差异有统计学意义(10.71±12.99vs 25.09±18.30,Z =-3.326,P=0.001),角膜荧光素着色评分较用药前明显降低,差异有统计学意义(2.24±2.36 vs 8.26±2.69,Z=-6.909,P＜0.01).0.1%透明质酸钠治疗4周患者的BUT(4.64±1.44)s 比治疗前(2.69±1.58)s明显延长,差异有统计学意义 (t=-5.899,P＜0.01),而Schirmer Ⅰ试验值(7.60±3.35)mm明显高于治疗前(5.81±3.57)mm,差异有统计学意义(t=-2.359,P=0.021). 随访过程中未发现任何不良反应.结论 0.1%透明质酸钠可通过促进LASIK术后角膜上皮的修复和改善泪液分泌量缓解LASIK术后干眼症患者的主觉症状。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129204463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

继发性Sj(o)gren综合征合并右眼泪道阻塞一例继发性Sj(o)gren综合征合并右眼泪道阻塞一例

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.002

庄宏, 徐建江, 朱文卿, 王文韬, 姜春晖

引用次数: 0

Expression of aquaporin-1 in human lens epithelial cells by hyperosmotic stimuli 高渗刺激下水孔蛋白-1在人晶状体上皮细胞中的表达

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.013

林雯, 谢茂松, 徐国兴, 何青, 郑卫东

{"title":"Expression of aquaporin-1 in human lens epithelial cells by hyperosmotic stimuli","authors":"林雯, 谢茂松, 徐国兴, 何青, 郑卫东","doi":"10.3969/J.ISSN.1003-0808.2010.08.013","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.08.013","url":null,"abstract":"目的研究水通道蛋白-1(AQP-1)在糖尿病性白内障发病机制中的作用.方法从健康的供体眼中分离出人晶状体囊膜,用组织块培养法将人晶状体囊膜分别置于1g/L葡萄糖+体积分数15%胎牛血清的DMEM培养液中(对照组)或4.5g/L葡萄糖+15%胎牛血清的DMEM培养液中进行培养(高糖组).培养3d、28d后观察2组人晶状体上皮细胞(LECs)的生长状态.采用免疫荧光技术和流式细胞术检测AQP-1在人LECs中表达的平均荧光强度,应用流式细胞术检测2组人LECs的凋亡和坏死情况,并进行比较.结果培养第3天2组LECs的生长形态近似,但培养28d高糖组坏死细胞增加.培养3d后高糖组人LECs的AQP-1免疫荧光表达较对照组明显增强,2组间平均荧光强度的差异有统计学意义(t=3.934,P=0.004),但2组间LECs的凋亡率和坏死率差异均无统计学意义(t=1.681,P=0.131;t=1.064,P=0.318).培养28d后高糖组人LECs的AQP-1免疫荧光表达较对照组弱,差异有统计学意义(t=3.069,P=0.015),LECs凋亡率、坏死率较对照组升高,差异均有统计学意义(t=11.213,P＜0.01;t=15.778,P＜0.01).结论 AQP-1的异常表达在糖尿病性白内障的发生发展中起重要作用。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124315034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advance in immunological mechanism after corneal alkali burns 角膜碱烧伤后免疫机制的研究进展

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.028

幸正茂

引用次数: 0

Promoting effect of PACAP38 on proliferation of trigeminal ganglion cells and growth of corneal nerve after flap surgery PACAP38对皮瓣术后三叉神经节细胞增殖及角膜神经生长的促进作用

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.003

赵秀丽, 陈建苏, 余榕捷, 丁勇, 郑佩娥, 李晓霞, 田振彩, 戴静

{"title":"Promoting effect of PACAP38 on proliferation of trigeminal ganglion cells and growth of corneal nerve after flap surgery","authors":"赵秀丽, 陈建苏, 余榕捷, 丁勇, 郑佩娥, 李晓霞, 田振彩, 戴静","doi":"10.3969/J.ISSN.1003-0808.2010.08.003","DOIUrl":"https://doi.org/10.3969/J.ISSN.1003-0808.2010.08.003","url":null,"abstract":"目的观察垂体腺苷酸环化酶激活肽(PACAP38)对培养的兔三叉神经节细胞及角膜瓣术后角膜神经生长的影响.方法从2周龄新西兰白兔中分离三叉神经节细胞,并在神经元基础培养液中进行培养.培养24h后在培养液中分别加入1μmol/L PACAP38(A组)、 0.1μmol/L PACAP38(B组),C组培养液中加入PBS.1周后行抗神经纤维丝蛋白(NF200)免疫荧光染色鉴别培养的三叉神经节细胞.27只新西兰白兔右眼行角膜瓣手术,并分为3组,术后1d分别以10μmol/L PACAP38、5μmol/L PACAP38或PBS点眼,共8周,行角膜敏感度测定和NF200免疫荧光染色,评价术后不同时间角膜神经的修复情况.结果 PACAP38培养的三叉神经节细胞有较多神经细胞存活,伸出突触并交织呈网状,A组神经细胞存活最多.角膜瓣手术的3个组角膜敏感度随着术后时间的延长逐渐增加(Ftime=58.196,P=0.00),10μmol/L PACAP38点眼组角膜敏感度恢复最快.10μmol/L PACAP38点眼组和5μmol/L PACAP38点眼组角膜敏感度值明显高于PBS点眼组,差异均有统计学意义(P=0.000,P=0.005).角膜神经染色示10μmol/L PACAP38点眼组角膜神经密度较5μmol/L PACAP38点眼组和PBS点眼组高,神经干短端多膨大,以出芽方式发出细微的新生神经纤维芽.结论 PACAP38能促进兔三叉神经节细胞增生及诱导神经突形成,可促进角膜敏感性恢复及角膜神经修复再生。","PeriodicalId":186994,"journal":{"name":"Chinese Ophthalmic Research","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121162030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

角膜地形图像差引导的个体化LASIK治疗角膜不规则散光角膜地形图像差引导的个体化LASIK治疗角膜不规则散光

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.012

张丰菊, 于芳蕾, 鲁智丽, 王越秀, 殷路

引用次数: 0

Exclusive mapping for all known loci in a family with congenital Cataract 先天性白内障家族中所有已知基因座的独家图谱

Chinese Ophthalmic Research Pub Date : 2010-08-10 DOI: 10.3969/J.ISSN.1003-0808.2010.08.015

梁小芳, 华芮, 石磊, 肖伟

引用次数: 0