Microscopy Research and Technique最新文献_第5页

Green Synthesis of Iron Oxide Nanoparticles for Treating Coffee Cherry Pulping Wastewater: Environmental Implications. 绿色合成氧化铁纳米颗粒处理咖啡樱桃制浆废水：环境影响。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-21 DOI: 10.1002/jemt.70047

Thamaraiselvi Chandran, Athira Sudarsanan Thulasi, Manikandan Elayaperumal, Fatimah S Al-Khattaf, Ashraf Atef Hatamleh, Kathikeyan Ravi, Soon Woong Chang, Balasubramani Ravindran

{"title":"Green Synthesis of Iron Oxide Nanoparticles for Treating Coffee Cherry Pulping Wastewater: Environmental Implications.","authors":"Thamaraiselvi Chandran, Athira Sudarsanan Thulasi, Manikandan Elayaperumal, Fatimah S Al-Khattaf, Ashraf Atef Hatamleh, Kathikeyan Ravi, Soon Woong Chang, Balasubramani Ravindran","doi":"10.1002/jemt.70047","DOIUrl":"https://doi.org/10.1002/jemt.70047","url":null,"abstract":"The disposal of coffee cherry pulping wastewater (CPWW) poses severe environmental risks, including water body eutrophication, soil acidification, and toxicity to aquatic life due to its high organic load (COD: 29,450 mg/L; BOD5: 16,500 mg/L), acidic pH (4.8), and elevated solids (TDS: 3240 mg/L; TSS: 4540 mg/L). To address this, we synthesized iron oxide nanoparticles from Ricinus communis L. seed extract via a green method and evaluated their efficacy in CPWW treatment. Characterization by XRD, SEM, and FTIR confirmed the formation of the nanoparticles stabilized by phytochemicals, with functional groups such as hydroxyl (OH) and amine (NH) critical for pollutant binding. At an optimal dosage of 0.08 g/100 mL, the nanoparticles achieved 74% COD removal, 69% TDS reduction, and 92% decolorization, significantly mitigating the pollutant load of the effluent, thereby minimizing risks to aquatic ecosystems and soil health. Furthermore, FTIR analysis of the treated sludge revealed the potential functional groups facilitating pollutant removal. This study highlights the dual environmental benefit of green-synthesized iron oxide nanoparticles: (1) a sustainable synthesis route avoiding toxic chemicals, and (2) high-efficiency pollutant removal, offering a scalable solution for coffee-processing industries to minimize their ecological footprint. By addressing the dual challenges of waste toxicity and treatment sustainability, this approach aligns with circular economy principles, promoting cleaner production in agro-industrial sectors.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PyCCAPT: A Python Package for Open-Source Atom Probe Instrument Control and Data Calibration. PyCCAPT：一个Python包用于开源原子探针仪器控制和数据校准。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-19 DOI: 10.1002/jemt.70011

Mehrpad Monajem, Benedict Ott, Jonas Heimerl, Stefan Meier, Peter Hommelhoff, Peter Felfer

{"title":"PyCCAPT: A Python Package for Open-Source Atom Probe Instrument Control and Data Calibration.","authors":"Mehrpad Monajem, Benedict Ott, Jonas Heimerl, Stefan Meier, Peter Hommelhoff, Peter Felfer","doi":"10.1002/jemt.70011","DOIUrl":"https://doi.org/10.1002/jemt.70011","url":null,"abstract":"Currently, the vast majority of atom probe instruments in use are commercial systems with closed, proprietary software. This is limiting for many experiments where low-level access to machine control, experiment data, or custom instrument setups is necessary. Over the past decade, advancements in off-the-shelf detector systems, fast data bus systems, and the availability of high-level programming languages such as Python have made it feasible to design and construct atom probe systems without extensive engineering expertise. Despite this progress, developing control system software, associated instruments, and data calibration algorithms remains a significant challenge for many projects. In this article, we introduce an atom probe control system that can be flexibly adapted to various hardware configurations. This system also includes essential instrument and experiment calibration algorithms, offering complete transparency to the user. This framework provides flexibility for innovative experiments and enhances calibration accuracy not possible with commercial systems. The methods and algorithms discussed are implemented in Python Control and Calibration for Atom Probe Tomography (PyCCAPT), which is an open-source solution for APT, addressing a gap in experimental control and data processing. While not compatible with commercial atom probes for data acquisition, its calibration module can be used for direct-flight-path systems and adapted for reflection-based instruments.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artemisia pallens-Mediated Manganese Nanoparticles: Apoptotic Effects on Human Epidermoid Carcinoma Cells and Their Antibiofilm Properties. 紫蒿介导的纳米锰对人表皮样癌细胞的凋亡作用及其抗生物膜特性。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-19 DOI: 10.1002/jemt.70049

Mohamed A El-Tayeb, Mais E Ahmed, Fatimah S Alkhattaf, Eman Alhomaidi, Penislusshiyan Sakayanathan

{"title":"Artemisia pallens-Mediated Manganese Nanoparticles: Apoptotic Effects on Human Epidermoid Carcinoma Cells and Their Antibiofilm Properties.","authors":"Mohamed A El-Tayeb, Mais E Ahmed, Fatimah S Alkhattaf, Eman Alhomaidi, Penislusshiyan Sakayanathan","doi":"10.1002/jemt.70049","DOIUrl":"https://doi.org/10.1002/jemt.70049","url":null,"abstract":"In recent times, green-synthesized nanoparticles have been widely used in the biomedical field due to their low toxicity, eco-friendliness, cost-effectiveness, and enhanced therapeutic potential. This study investigated the anticancer, antibacterial, and antibiofilm properties of green-synthesized manganese dioxide nanoparticles derived from Artemisia pallens (AP-MnNPs). The AP-MnNPs were characterized through various techniques, including UV-Vis spectroscopy, SEM, EDX, XRD, and FTIR. These nanoparticles effectively inhibited the growth of cancer cell lines A431, A549, and MCF7, with the highest inhibition observed in A431 cells (IC50 = 25 μg/mL). In A431 cells, AP-MnNPs induced ROS generation, mitochondrial disruption, arrested cell cycle progression at the G2/M phase, DNA breakage, and programmed cell death. AP-MnNPs also modulated apoptotic markers by increasing the expression of pro-apoptotic proteins (Bax, caspase 3) and decreasing the anti-apoptotic protein Bcl-2, thereby disrupting cell proliferation via suppression of the PI3K/Akt signaling pathway. Furthermore, AP-MnNPs demonstrated antibacterial and antibiofilm efficacy against Pseudomonas aeruginosa, Streptococcus mutans, Staphylococcus aureus, and their mixed cultures. Collectively, AP-MnNPs exhibit promising potential as anticancer agents that impede cancer cell growth and modulate proliferation-related signaling proteins, as well as antibacterial and antibiofilm agents.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From Chromosomes to Seeds: Meiotic Behavior and Its Effect on the Reproductive Outcomes in Phytolacca acinosa Roxb., a Multipurpose Medicinal Herb From the Himalaya. 从染色体到种子：植物减数分裂行为及其对生殖结果的影响。一种产自喜马拉雅山的多用途草药。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-17 DOI: 10.1002/jemt.70045

Junaid A Magray, Bilal A Wani, Aijaz H Ganie, Hanan Javid, Roof Ul Qadir, Irshad A Nawchoo

{"title":"From Chromosomes to Seeds: Meiotic Behavior and Its Effect on the Reproductive Outcomes in Phytolacca acinosa Roxb., a Multipurpose Medicinal Herb From the Himalaya.","authors":"Junaid A Magray, Bilal A Wani, Aijaz H Ganie, Hanan Javid, Roof Ul Qadir, Irshad A Nawchoo","doi":"10.1002/jemt.70045","DOIUrl":"https://doi.org/10.1002/jemt.70045","url":null,"abstract":"Understanding meiotic behavior and pollen biology is crucial for effective conservation of plant species. This study presents the first detailed analysis of meiotic behavior and its correlation with pollen fertility and seed set in Phytolacca acinosa Roxb., a multipurpose medicinal herb native to the Himalaya. The flower buds were collected from two selected sites in the Kashmir Himalaya, India. In this study, we used the squash technique, in which anthers were squashed in 2% acetocarmine, and the slides were observed under a microscope to assess chromosome number and meiotic behavior. The species exhibited a chromosome count of 2n = 8x = 72. Most pollen mother cells (PMCs) (74%-79%) underwent normal meiosis; however, 21%-26% PMCs exhibited abnormalities, including chromosome stickiness, chromosomal bridges, laggards, un-oriented bivalents, and micronuclei. Chromosome stickiness was the most prominent meiotic abnormality, followed by laggard and bridge formation. These meiotic irregularities significantly reduced pollen fertility (75.45%-77.57%), fruit set (73.22%-75.67%), and seed set (71.1%-72.14%) across both selected sites. The findings highlight meiotic irregularities as a potential reproductive constraint, emphasizing the need for conservation measures to ensure the sustainable utilization of this medicinally important plant species. Future studies should investigate the genetic and environmental factors underlying these meiotic disturbances.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green Synthesis and Characterization of Silver Nanoparticles Using Lespedeza juncea Extract: An Insight Into Its Antibacterial, Antifungal, and Enzyme Inhibitory Potential. 胡枝子提取物绿色合成及纳米银的表征：对其抗菌、抗真菌和酶抑制潜能的研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-16 DOI: 10.1002/jemt.70040

Muhammad Irshad, Luqman Shah, Nodia Shujaat, Majid Khan, Zeeshan Niaz, Ikram Ullah

{"title":"Green Synthesis and Characterization of Silver Nanoparticles Using Lespedeza juncea Extract: An Insight Into Its Antibacterial, Antifungal, and Enzyme Inhibitory Potential.","authors":"Muhammad Irshad, Luqman Shah, Nodia Shujaat, Majid Khan, Zeeshan Niaz, Ikram Ullah","doi":"10.1002/jemt.70040","DOIUrl":"https://doi.org/10.1002/jemt.70040","url":null,"abstract":"Herein, we adapted a quick, cost-effective, and environmentally friendly biological method for the synthesis of Ag-NPs using silver nitrate solution as a precursor, and the aqueous leaf extract of Lespedeza juncea (Chinese lespedeza) was exploited to synthesize Ag-NPs. Various physiochemical characterization techniques were used to characterize the nanoparticles. The UV spectrum revealed a resonance absorption peak at 428 nm, indicating successful synthesis of the nanoparticles. The EDX results indicated the presence of Ag, C, O, and Cl elements in biosynthesized Ag-NPs with elemental compositions of 53.93%, 32.34%, 12.49%, and 1.23%, respectively. The XRD analysis displayed the crystalline nature of the nanoparticles with a face-centered cubic lattice. The FTIR spectrum confirmed the involvement of plant-based biological compounds as reducing and capping agents. TEM revealed quasi-spherical Ag-NPs of 50 nm or smaller in size. An insight into its biological activities reveals significant antimicrobial activities against Staphylococcus aureus (14.1 ± 0.76 mm), Pseudomonas aeruginosa (11.50 ± 0.40 mm), Escherichia coli (16 ± 0.95 mm), and the fungal strains, viz. Candida albicans (14 ± 0.80 mm), Aspergillus flavus (16.50 ± 0.50 mm), Aspergillus niger (17 ± 0.86 mm) and afflicted 75% mortality to Caenorhabditis elegans. Furthermore, the Ag-NPs were found to be potent inhibitors of the enzymes tyrosinase, urease, acetylcholinesterase, and butyrylcholinesterase with IC50 values of 14.3 ± 0.2, 19.5 ± 1.1, 9.3 ± 1.3, and 32.65 ± 1.9 μg/mL, respectively. The overall outcome of the study suggests that L. juncea mediated synthesized Ag-NPs hold the potential to be employed as a promising tool for their antibacterial, antifungal, nematocidal, and for a variety of enzymes inhibitory activities.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Gallium Focused Ion Beam on Polyethylene: Study of Molecular Damage. 镓聚焦离子束对聚乙烯分子损伤的影响。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-16 DOI: 10.1002/jemt.70044

Maciej Paśniewski, Claude Poleunis, Arnaud Delcorte, Rachel Terry, David W Abmayr, Anton-Jan Bons, Dominique Schryvers

{"title":"Influence of Gallium Focused Ion Beam on Polyethylene: Study of Molecular Damage.","authors":"Maciej Paśniewski, Claude Poleunis, Arnaud Delcorte, Rachel Terry, David W Abmayr, Anton-Jan Bons, Dominique Schryvers","doi":"10.1002/jemt.70044","DOIUrl":"https://doi.org/10.1002/jemt.70044","url":null,"abstract":"Focused ion beam-scanning electron microscopy (FIB-SEM) is a microscopy technique that can be used to investigate the quality and structural properties of industrial materials such as polyolefins. An understudied aspect and possible drawback of the technique could be the implantation of the impinging ions under the sample surface and damage to the molecular structure, hindering its use as a sample preparation tool for surface-sensitive techniques. We systematically investigated the damaging effects of gallium liquid metal focused ion beam under grazing incident beam angle and various accelerating voltages on polyethylene. Changes in molecular structure and ion implantation depth were analyzed with Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) depth profiling and optical profilometry. Our results show that the gallium ion beam causes significant damage to the polyolefin structure, which is especially observed as dehydrogenation of the molecular structure of the (sub)surface. These molecular products are concentrated in distinct sub-surface zones, where damage is coupled to the presence of implanted gallium from the FIB etching.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Morphological Studies on the Oropharyngeal Roof of the Adult Little Owl and Domestic Pigeon. 成年小猫头鹰与家鸽口咽顶的比较形态学研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-14 DOI: 10.1002/jemt.70043

Radwa A Ali, Mohammed Abdelsabour-Khalaf, Soheir A Rabie, Mohamed A A Mahdy

{"title":"Comparative Morphological Studies on the Oropharyngeal Roof of the Adult Little Owl and Domestic Pigeon.","authors":"Radwa A Ali, Mohammed Abdelsabour-Khalaf, Soheir A Rabie, Mohamed A A Mahdy","doi":"10.1002/jemt.70043","DOIUrl":"https://doi.org/10.1002/jemt.70043","url":null,"abstract":"The current study aims to compare the morphological features of the oropharyngeal roof of the little owl (Athene noctua) and the domestic pigeon (Columba livia domestica) by gross anatomy, morphometric measurements, scanning electron microscopy, and light microscopy. Ten heads of normal, healthy, and adult little owls and domestic pigeons were used in the current study as follows: seven heads for gross anatomical examination and light microscopy, and three for scanning electron microscopy. The oropharyngeal roof had a triangular appearance in both birds, was enlarged and broad in the little owl, and narrowly elongated in the domestic pigeon. The little owl had a median longitudinal ridge, a transverse ridge, and two lateral longitudinal ridges, while the domestic pigeon had one median and two lateral ridges. The choanal slit in both birds was composed of rostrally narrow and caudally wide parts. In the little owl, the edges of both parts were encircled by papillae, while in the domestic pigeon, the papillae encircled the edges of the rostral part only. The surface of the pharyngeal roof in the little owl had numerous elongated conical pharyngeal papillae, while that of the domestic pigeon was devoid of papillae. The edges of the infundibular cleft in the little owl had prominent large conical papillae, while those in the pigeon were devoid of papillae. Histologically, the epithelium of the plate and pharynx of both birds was composed of stratified squamous epithelium. In conclusion, the present study clarified several differences between the oropharyngeal roof of the little owl and the domestic pigeon, which reflect their adaptation to their feeding habits and the surrounding environments.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gel Containing Catechin and Mesoporous Silica Nanoparticles for Protecting Root Dentin Against Erosion: An In Situ Study. 凝胶含有儿茶素和介孔二氧化硅纳米颗粒保护根牙本质免受侵蚀：一项原位研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-14 DOI: 10.1002/jemt.70042

Helaine Cajado Alves, Edison Augusto Balreira Gomes, Antonia Flavia Justino Uchoa, Nágila Maria Pontes Silva Ricardo, Vanara Florêncio Passos, Sérgio Lima Santiago

{"title":"Gel Containing Catechin and Mesoporous Silica Nanoparticles for Protecting Root Dentin Against Erosion: An In Situ Study.","authors":"Helaine Cajado Alves, Edison Augusto Balreira Gomes, Antonia Flavia Justino Uchoa, Nágila Maria Pontes Silva Ricardo, Vanara Florêncio Passos, Sérgio Lima Santiago","doi":"10.1002/jemt.70042","DOIUrl":"https://doi.org/10.1002/jemt.70042","url":null,"abstract":"This study evaluated the in situ anti-erosive effect of gels containing epigallocatechin-3-gallate (EGCG) isolated and adsorbed on mesoporous silica nanoparticles (EGCG/MSN) on eroded dentin. Eleven volunteers participated in this randomized, controlled, cross-over study, which consisted of 4 phases of 5 days. Acrylic palatal devices were utilized containing two dentin blocks treated with one of the gels: placebo (negative control), SnF2 (0.05%-positive control), EGCG (0.1%), and EGCG/MSN (0.093%). The specimens were immersed in citric acid (0.05 M; pH 3.75) for 60 s, 4×/day, followed by treatment with the assigned gel for 60 s. The alterations were evaluated by the percentage of surface hardness loss (%SHL) and through profilometry analysis (wear). Morphological changes were assessed using scanning electron microscopy (SEM). The data were analyzed using ANOVA, followed by Tukey's post-test. The %SHL did not show a significant difference among the groups. Regarding surface wear, the mean results in micrometers were: placebo, 0.66 (±0.38); EGCG, 0.57 (±0.11); EGCG/MSN, 0.48 (±0.05); and SnF2, 0.32 (±0.08). A significant difference was observed between the SnF2 group and the placebo and EGCG groups. However, there were no difference between the EGCG/MSN group and the control ones. Within the limitations of the study, EGCG/MSN may act as a protective measure in reducing dentin wear under erosive conditions since it did not differ from the positive control.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction. 发光卫星细胞：光片荧光显微镜用于小鼠离体强迫偏心收缩损伤的骨骼肌三维成像。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-14 DOI: 10.1002/jemt.70046

Rachele Garella, Elisa Imbimbo, Francesco Palmieri, Alessia Tani, Martina Parigi, Flaminia Chellini, Alessandra La Contana, Monica Mattioli Belmonte, Aurora Longhin, Ludovico Silvestri, Chiara Sassoli, Roberta Squecco

{"title":"Illuminating Satellite Cells: Light Sheet Fluorescence Microscopy for 3D Imaging of Murine Skeletal Muscles Damaged by Ex Vivo Forced Eccentric Contraction.","authors":"Rachele Garella, Elisa Imbimbo, Francesco Palmieri, Alessia Tani, Martina Parigi, Flaminia Chellini, Alessandra La Contana, Monica Mattioli Belmonte, Aurora Longhin, Ludovico Silvestri, Chiara Sassoli, Roberta Squecco","doi":"10.1002/jemt.70046","DOIUrl":"https://doi.org/10.1002/jemt.70046","url":null,"abstract":"In this letter, we put forward the light sheet fluorescence microscopy (LSFM) as a cutting-edge tool for 3D imaging of whole skeletal muscle, focusing on satellite cells (SCs). SCs represent the resident adult muscle stem cells, normally lying quiescent between the sarcolemma of the myofiber and the surrounding basal lamina. They typically express Pax-7 and, when activated following damage, they sequentially express specific myogenic regulatory factors including the myogenic determination factor, MyoD, thus starting differentiation towards multinucleated myofibers to repair injured tissue. The present analysis was performed on an ex vivo model of murine skeletal muscle injured by a forced eccentric contraction in isometric condition. The entire muscles were subjected to a tissue clearing and whole-mount staining process, enabling optical access and specific labeling across the entire intact sample. We performed labeling either with a fluorescent analog of standard hematoxylin and eosin, or with specific immunostaining against Pax-7 and MyoD. This proof of concept study demonstrates the feasibility of whole-muscle imaging with LSFM for the evaluation of the spatial arrangement of resting and activated SCs, overcoming the methodological limits of conventional 2D histology. This innovative experimental pipeline can be useful to test novel therapeutic approaches aimed at enhancing tissue regeneration and other biomedical/clinical applications.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multimodal OF-MTMFL: A Semi-Supervised Mean Teacher Model for Histopathological Image Segmentation. 多模态OF-MTMFL：用于组织病理图像分割的半监督平均教师模型。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-14 DOI: 10.1002/jemt.70039

R Christal Jebi

{"title":"Multimodal OF-MTMFL: A Semi-Supervised Mean Teacher Model for Histopathological Image Segmentation.","authors":"R Christal Jebi","doi":"10.1002/jemt.70039","DOIUrl":"https://doi.org/10.1002/jemt.70039","url":null,"abstract":"In the rapidly advancing field of histopathological image analysis, accurate segmentation of critical features is crucial for medical diagnostics, as it enables pathologists to make precise decisions. The proposed One Former-based Mean Teacher Model with Federated Learning (OF-MTMFL) system combines cutting-edge semi-supervised learning and federated learning techniques to tackle issues such as limited annotated data and class imbalance. The framework utilizes a mean teacher architecture, where the student model, guided by a focal loss function, prioritizes high-confidence regions in unlabeled data, while the teacher model ensures consistency through Exponential Moving Average (EMA) updates. To further enhance segmentation accuracy, multi-scale attention modules are employed for robust feature extraction. Additionally, the system incorporates a Federated Learning mechanism that allows multiple institutions to collaborate without sharing raw data, including datasets from the Cancer Genome Atlas (TCGA). The results from the analysis of the TCGA dataset indicate that the proposed OF-MTMFL model achieved mean concordance index (c-index) scores of 0.700 ± 0.030 for Bladder Urothelial Carcinoma (BLCA), 0.720 ± 0.040 for Breast Invasive Carcinoma (BRCA), 0.860 ± 0.025 for Glioblastoma & Lower Grade Glioma (GBMLGG), 0.690 ± 0.035 for Lung Adenocarcinoma (LUAD), and 0.740 ± 0.045 for Uterine Corpus Endometrial Carcinoma (UCEC). The overall performance score of the OF-MTMFL model across these cancer types is 0.740, demonstrating particularly strong results in GBMLGG while maintaining competitive scores in the other cancer types. The standard deviations reported reflect the variability of the model's performance across different samples within each category.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144626624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0