Microscopy Research and Technique最新文献

{"title":"Juglone as a Therapeutic Agent in Murine Coccidiosis: Enhancing Intestinal Recovery and Regulating Host Immune Responses.","authors":"Shaimaa M Kasem, Mohamed A Dkhil, Amal M Marey, Rewaida Abdel-Gaber, Felwa A Thagfan, Sherif Elshanat","doi":"10.1002/jemt.24871","DOIUrl":"https://doi.org/10.1002/jemt.24871","url":null,"abstract":"<p><p>Coccidiosis, caused by Eimeria spp., significantly impacts gastrointestinal health and leads to economic losses. This study investigated juglone, a phenolic compound, as a potential treatment for mouse coccidiosis caused by Eimeria papillata. Twenty-five male Swiss albino mice were categorized into five groups: Group 1: non-infected, Group 2: juglone control (JG), Group 3: infected with 1,000 sporulated oocysts, Group 4: infected-treated with juglone (Infected + JG), and Group 5: infected-treated with anticoccidial drug, amprolium (Infected + ACD). Following 5 days of treatment, the mice were euthanized, and numerous analyses were performed such as growth performance, oocyst shedding, oxidative stress, histology, goblet cell response, and inflammation. Results showed that E. papillata infection significantly decreased body weight and feed intake, increased oocyst shedding, and elevated oxidative stress markers while reducing antioxidant enzyme levels. Histopathological analysis indicated severe intestinal damage, with intracellular parasitic stages and reduced goblet cell density and MUC-2 expression. Infection also heightened gene expression of inflammatory cytokines including tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin 1β (IL1β), and interleukin 10 (IL10). Juglone treatment effectively mitigated these negative effects, improving body weight and feed consumption, lowering oocyst shedding, and restoring antioxidant enzyme activity. It also repaired intestinal structure, enhanced goblet cell density and MUC-2 expression, as well as diminished inflammatory cytokines. The findings indicated that juglone significantly mitigates the pathological effects induced by E. papillata by decreasing parasite shedding, oxidative stress, inflammation, and modulating immune responses while facilitating intestinal recovery and enhancing overall health in infected mice.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards Understanding the Effect of Loading Direction on Fatigue Crack Behavior in Mg-3Al-Zn Magnesium Alloy.","authors":"Zhanghu Shi, Pan Tang, Hua Rao, Pei Lei, Deng Pan, Junshan Hu, Wei Tian","doi":"10.1002/jemt.24869","DOIUrl":"https://doi.org/10.1002/jemt.24869","url":null,"abstract":"<p><p>As the lightest structural metal material, magnesium alloy is increasingly widely used in aerospace, rail transportation and other fields. However, during its service, the action of alternating loads often induces fatigue fracture damage, which seriously threatens its service safety and stability. This study investigates the fatigue behaviors of Mg-3Al-Zn (AZ31) magnesium alloy along its rolling direction (RD) and normal direction (ND) under strain-controlled tension-tension cyclic loading. The strain-life curve reveals that ND specimens in which the deformation was dominated by twinning-detwinning exhibits higher fatigue life than RD specimens dominated by slip. Morphological analysis of crack patterns indicates many grain boundary fractures and long surface cracks in RD specimens, contrasting with the ND specimens' uniform distribution of short, linear cracks throughout the texture. Scanning Electron Microscopy (SEM) and Electron Backscatter Diffraction (EBSD) analyses suggest that crack initiation in RD specimens is likely at grain boundaries due to deformation incompatibility between the grains with soft and hard directions. Conversely, the cracks of ND specimen predominantly formed at the {10 <math> <semantics> <mrow><mover><mn>1</mn> <mo>¯</mo></mover> </mrow> <annotation>$$ overline{1} $$</annotation></semantics> </math> 2} twinning boundaries, owing to the strain incompatibility between the matrix and twin due to the discrepancy of basal dislocation activations.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Architecture and Morphological Examination of Hirudo verbana (Annelida, Hirudinea).","authors":"Sedef Selviler Sizer, Hakan Eren, Cansu Aktaş, Mehmet Pazar, Esra Demirbas, Burcu Onuk, Ertan Emek Onuk, Gokmen Zafer Pekmezci, Murat Kabak","doi":"10.1002/jemt.24859","DOIUrl":"https://doi.org/10.1002/jemt.24859","url":null,"abstract":"<p><p>In this study, the morphological characteristics of the medicinal leech Hirudo verbana (Annelida, Hirudinea) were examined. Mitochondrial CO1 gene sequences of representative specimens were recorded in GenBank, and macroscopic, histological, and SEM analyses of remaining representatives were made. The species had trignathous jaws, each half-moon in shape. There were 75-80 denticles on the left jaw with their tips medially, while the right jaw had 30-34 equilateral triangular denticles, all arranged in a single row. The anterior jaw had 35-40 blunt-tipped denticles similarly arranged in a single row. Salivary papillae of varying sizes were scattered along the edges of the jaws, with salivary pores appearing as irregularly distributed round openings. Five pairs of dorsal eyes appeared round under a stereomicroscope but oval under SEM. Seventeen pairs of round nephridial pores were symmetrically located on every fifth annulus of the ventral side. Grape-cluster-like cells containing granules were observed between the crop and the muscle layer. Bean-shaped testisacs and coiled vas deferens canals were bilaterally arranged near the crop. Thread-like extensions and sensilla were identified across the ventral annuli. Beneath the epidermis, circular, oblique, and longitudinal muscle layers and three types of secretory cells between them were identified. As a result, this study has identified differences in the denticle morphology of H. verbana and has contributed these findings to the literature. Additionally, it was found that there are differences in the shape of the examined structures between stereomicroscope and SEM images in small and complexly structured organisms.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternatives for Uranyl Acetate Negative Staining With Respect to the Resolution Obtained After Single Particle Analysis of Erythrocruorin.","authors":"Foteini Karapanagioti, Nicolas Cissé, Anastasiia Atamas, Artem Stetsenko, Christiaan Michiel Punter, Erica Zuidersma, Marc C A Stuart","doi":"10.1002/jemt.24865","DOIUrl":"https://doi.org/10.1002/jemt.24865","url":null,"abstract":"<p><p>Although cryo-electron microscopy is widely used for determining high-resolution structures, negative staining of proteins and supramolecular aggregates remain a valuable screening method, especially for low-abundant proteins. The most commonly used contrasting agent is uranyl acetate (UAc). However, due to its toxicity and the strong regulations connected to the use of uranium compounds, there is an ongoing search for alternative stains, with the focus being shifted to lanthanide and tungsten compounds. In this study, the performance of neodymium acetate (NdAc), europium acetate (EuAc), ytterbium acetate (YbAc), phosphotungstic acid, and the commercially available UA-Zero as negative staining agents for single molecule electron microscopy is evaluated. We focus not only on the observed contrast but also on the resolution that can be obtained after negative staining and single-particle analysis. The findings demonstrate that NdAc, EuAc, and UA-Zero can be good alternatives for the overall superior UAc. Additionally, we explore the working pH range of the stains and discuss the usability of the compounds in terms of availability and regulations.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocatalytic Degradation of Synozol Navy Blue Dye by Ni-Cu-Ag Dopped ZnO: An Insight Into Photoluminescence, Scavenging, Mechanism and Kinetic Aspects.","authors":"Zahid Mahmood, Muhammad Alamzeb, Tahseen Ghous, Sidra Rashid, Zulfiqar Ali","doi":"10.1002/jemt.24867","DOIUrl":"https://doi.org/10.1002/jemt.24867","url":null,"abstract":"<p><p>Cost-effective, sustainable, and eco-friendly strategies are crucial for treating organic pollutants in industrial wastewater. ZnO is a promising photocatalyst due to its affordability, environmentally benign nature, and ability to fully mineralize pollutants. However, its limited effectiveness under visible light is a major drawback. Ni doping helps reduce charge carrier recombination, enhancing the photocatalytic activity of ZnO. Ni-Cu co-doping increases saturation magnetization by creating intrinsic defects, though it can lead to nanocluster formation on the catalyst surface. Ag further improves the performance by preventing nanocluster aggregation and prolonging charge carrier lifetimes. In this study, Ni-Cu-Ag@ZnO nanoparticles were synthesized via the sol-gel method and used as a photocatalyst for the degradation of Synozol navy blue dye. Characterization techniques like FTIR, SEM, XRD, EDX, BET, UV, and PL confirmed the nanocomposite structure and morphology. The Ni-Cu-Ag@ZnO nanocomposite achieved 88% photodegradation efficiency under optimal conditions (a contact time of 50 min, an initial dye concentration of 75 ppm, a catalyst dosage of 0.03 g, and an acidic medium with a pH of 2). The rate constants, k₁ (2 × 10^-2 min^-1) suggest that the degradation of Synozol navy blue by Ni-Cu-Ag@ZnO under ordinary light follows pseudo-first-order kinetics. Scavenging tests further confirmed that the enhanced photocatalytic degradation was driven by the generation of OH^• radicals, with the addition of hydrogen peroxide playing a key role in preventing electron-hole recombination. This indicates that the quantity of hydroxyl radicals and their adsorption on the photocatalyst are crucial in determining the extent of degradation. This study highlights the potential of Ni-Cu-Ag@ZnO nanocomposites for industrial wastewater treatment and opens new avenues for the further enhancement of the photocatalytic efficiency of other catalysts under consideration.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Caries Eradication Techniques via Papacarie, and Er: YAG Laser With Different Adhesive Types Bonded to Composite Restoration in Primary Dentin. An SEM, μTBS, and Microleakage Assessment.","authors":"Faisal Ali Bin Abbooud AlQhtani","doi":"10.1002/jemt.24863","DOIUrl":"https://doi.org/10.1002/jemt.24863","url":null,"abstract":"<p><p>Influence of different caries removal techniques using Papacarie and Erbium/yttrium-aluminum-garnet (Er: YAG) laser to different adhesives etch and rinse (ER) and self-etch (SE) restored via tooth-colored material on micro tensile bond strength (μTBS) and marginal leakage scores. Ninety-six extracted primary molars exhibiting moderate caries were allocated into three groups based on the caries removal technique. Group 1: Conventional bur, Group 2: Papacarie gel, and Group 3: Er: YAG laser. After caries removal, specimens from three groups were further allocated into two subgroups based on the type of adhesive used (n = 16). Two-step ER (A) and Two-step SE (B). Composite restoration was built and thermocycling was performed. Microleakage, μTBS, and failure mode were analyzed using a dye penetration test, a Universal testing machine, and a stereomicroscope respectively. Scanning electron microscopy (SEM) evaluation of the composite-CAD interface was performed. One-way ANOVA and the Tukey HSD test were utilized to compare microleakage and μTBS among investigated groups (p < 0.05). The lowest microlekage (13.56 ± 0.02) and highest μTBS (13.26 ± 0.11 MPa) was exhibited by Group 3B (Er: YAG laser-SE). However, the highest microleakage (45.62 ± 0.09) and lowest bond strength (9.34 ± 0.11 MPa) was exhibited by 1A (Conventional bur-ER) treated samples. For caries removal in primary dentition, the Er: YAG laser and Papacarié gel present themselves as appropriate alternatives. These non-invasive methods yield improved mechanical results when utilized with self-etch adhesive system.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Morphometry of Mitochondria: Comparison Between Conventional Operator-Dependent and Artificial Intelligence (AI)-Operated Machine Learning Methods.","authors":"Daniele Nosi, Daniele Guasti, Alessia Tani, Sara Germano, Daniele Bani","doi":"10.1002/jemt.24866","DOIUrl":"https://doi.org/10.1002/jemt.24866","url":null,"abstract":"<p><p>Morphometric analysis of digital images is fundamental to substantiate the visual observations with objective quantitative data suitable for statistical analysis. The recent advances in artificial intelligence (AI) have allowed the development of machine learning (ML) protocols for automated morphometry. Transmission electron microscopy (TEM) morphometry requires that the ultrastructural details be recognized and interpreted by a trained observer; this makes adapting AI-operated protocols to TEM particularly challenging. In this study, we have checked the accuracy of the results of mitochondrial morphometry yielded by a ML method by comparison with those obtained manually by a trained observer on the same TEM micrographs (magnification ×50,000) of cultured cells with different energy metabolism (overall n = 26). The measured parameter was the ratio between the total length of the mitochondrial cristae and the corresponding mitochondrial surface area (C/A ratio), directly related to mitochondrial function. No statistically significant correlation (Pearson's test) was found between the two methods in any of the experiments. Only in a few micrographs were the values similar (n = 3) or very close (n = 2) to be comprised within the s.e.m. of their experimental group. Moreover, as judged by the s.d. comparison, the scatter of values was more prominent with the ML-operated than with the manual method. Conceivably, this outcome is because many ultrastructural details of the cell organelles are similar, for example, the membrane section profiles, and can only be properly recognized and distinguished by an experienced observer, while the current ML protocols still cannot.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis, Microstructural Characterization, and Synergizing Effect of Na-β-Type Hexagonal Ferrite Nanomaterials as a Probe for Cefixime Electrochemical Sensors.","authors":"Sajjad Hussain, Imran Sadiq, Jameel Ahmed Baig, Shakoor Ahmed Solangi, Zahid U Khan, Imam Bakhsh Solangi, Saira Riaz, Robert C Pullar, Khalid Hussain Thebo, Mohsin Kazi","doi":"10.1002/jemt.24810","DOIUrl":"https://doi.org/10.1002/jemt.24810","url":null,"abstract":"<p><p>The quantitative study of antibiotics is important in various biomedical applications. Cefixime is a third-generation cephalosporin medication used to treat a range of bacterial infections. In this study, the green synthesis of sodium β-type hexagonal ferrite nanomaterial (NaFe₁₁O₁₇-NMs) was carried out from ginger root extract via the sol-gel method. The as-synthesized NMs were characterized via atomic force microscopy (AFM), field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM) analysis, and UV-visible spectroscopy. Furthermore, the drop-casting process is used to synthesize an efficient electrochemical sensor by coating a gold electrode (AuE) with NaFe₁₁O₁₇ NMs (NaFe₁₁O₁₇-NMs/AuE), which helps in the sensitive and selective quantification of cefixime. The as-prepared electrochemical process had a remarkably low detection limit of 14 nM. The as-fabricated sensor was effectively used to quantify cefixime levels in clinical samples and pharmaceutical formulations, with recoveries ranging from 95.20% to 102.48%.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscope-Assisted Hypertensive Retinopathy Diagnosis Using Deep Learning Models.","authors":"Shahzad Akbar, Usama Shahzore, Tanzila Saba, Faten S Alamri, Sadaf S Khan, Amjad R Khan","doi":"10.1002/jemt.24847","DOIUrl":"https://doi.org/10.1002/jemt.24847","url":null,"abstract":"<p><p>The retina is the most crucial part of the human eye, and it can be affected due to hypertension. However, retinal abnormalities due to hypertension are termed hypertensive retinopathy (HR). A severe stage of HR can lead to complete blindness if not diagnosed and treated on time. Manually analyzing retinal images for HR diagnosis is time-consuming and prone to errors. This research article provides a novel technique based on U-Net and Dense-Net for automatic HR detection and grading through retinal images. The presented method consists of preprocessing, vessel segmentation, artery or vein (A/V) classification, and vessel width calculation to compute the arteriovenous ratio (AVR). In the preprocessing phase, the Gabor filter is applied to the retinal image to enhance the vascular network of the image. The preprocessed image is fed into the U-Net architecture to segment the vascular network image. The segmented vascular network image is fed into the Dense-Net architecture for A/V classification. The A/V classified vascular network is divided into several artery and vein segments at the bifurcation and crossover points. The A/V segments are labeled for width calculation to compute the AVR. The AVR is a standard parameter for HR detection and grading. The evaluation results show an average accuracy of 99.40% in HR classification and 99.77% in HR grading on the AVRDB dataset. The evaluated results are beneficial for the automatic HR detection and grading for clinical purposes.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143701018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Multi-Modal Approach for Exploring Sarcoma and Carcinoma Using FTIR and Polarimetric Analysis.","authors":"Muhammad Abubakar Siddique, Munir Akhtar, Muhammad Abdul Majid, Ejaz Ahmad Khera, Manzoor Ahmad, Ghulam Gilanie, Wesam Atef Hatamleh, Muhammad Bilawal Junaid, Muhammad Umar Dad, Hafeez Ullah","doi":"10.1002/jemt.24853","DOIUrl":"https://doi.org/10.1002/jemt.24853","url":null,"abstract":"<p><p>Ex vivo characterization of sarcoma and carcinoma tissue samples was evaluated using microscopy, optical polarimetry, Fourier transform infrared (FTIR) spectroscopy, and support vector machines (SVM). Recent evidence suggests that it is crucial to explore new diagnostic methods for detecting the smallest features of cancer. In this paper, we discuss the FTIR, which characterizes the chemical composition of sarcoma and carcinoma tissues at different wavenumbers. The FTIR spectra of sarcoma tissues exhibited significant differences in chemical composition (OH, CH, and NH) compared to carcinoma tissues (NH, CO and CH), particularly in the spectral range from 400 to 4000 cm^-1. Mueller matrix polarimetry (MMP) combined with polar decomposition was used to compare 13 polarimetric parameters in ex vivo sarcoma and carcinoma tissues across the visible spectrum (400-800 nm), revealing significantly higher values for all metrics in sarcoma samples. Microscopic analysis revealed distinctive morphological changes associated with sarcoma and carcinoma, contributing to these variations. All polarimetric features explored using SVM demonstrated promise for computer-assisted classification of the two tissue types. SVM successfully achieved an overall 90% accuracy, sensitivity, and specificity. These results suggest that the combination of optical polarimetry and FTIR, along with SVM, holds significant potential for automated pathology classification of sarcoma and carcinoma.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}