Microscopy Research and Technique最新文献

{"title":"An accurate paradigm for denoising degraded ultrasound images based on artificial intelligence systems.","authors":"F E Al-Tahhan, M E Fares","doi":"10.1002/jemt.24675","DOIUrl":"10.1002/jemt.24675","url":null,"abstract":"<p><p>Ultrasound images are susceptible to various forms of quality degradation that negatively impact diagnosis. Common degradations include speckle noise, Gaussian noise, salt and pepper noise, and blurring. This research proposes an accurate ultrasound image denoising strategy based on firstly detecting the noise type, then, suitable denoising methods can be applied for each corruption. The technique depends on convolutional neural networks to categorize the type of noise affecting an input ultrasound image. Pre-trained convolutional neural network models including GoogleNet, VGG-19, AlexNet and AlexNet-support vector machine (SVM) are developed and trained to perform this classification. A dataset of 782 numerically generated ultrasound images across different diseases and noise types is utilized for model training and evaluation. Results show AlexNet-SVM achieves the highest accuracy of 99.2% in classifying noise types. The results indicate that, the present technique is considered one of the top-performing models is then applied to real ultrasound images with different noise corruptions to demonstrate efficacy of the proposed detect-then-denoise system. RESEARCH HIGHLIGHTS: Proposes an accurate ultrasound image denoising strategy based on detecting noise type first. Uses pre-trained convolutional neural networks to categorize noise type in input images. Evaluates GoogleNet, VGG-19, AlexNet, and AlexNet-support vector machine (SVM) models on a dataset of 782 synthetic ultrasound images. AlexNet-SVM achieves highest accuracy of 99.2% in classifying noise types. Demonstrates efficacy of the proposed detect-then-denoise system on real ultrasound images.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141982757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palynological investigation of some genera of Cichorieae (Asteraceae) in Iran.","authors":"Maneezheh Pakravan, Seyed Reza Safavi, Roghaieh Zarei, Zahra Tavakoli, Farrokh Ghahremaninejad, Maryam Noroozi, Zahra Nazem Bokaee, Mina Alimadadi, Najmeh Ghodusian, Fatemeh Hosseini, Fereshteh Moradi","doi":"10.1002/jemt.24663","DOIUrl":"10.1002/jemt.24663","url":null,"abstract":"<p><p>The pollen morphology of 33 species belonging to the genera Lactuca, Launaea, Cicerbita, Sonchus, Condrilla (tribe Cichorieae: Asteraceae) from Iran were studied with light and electron microscopies. The pollen grains of the investigated taxa are almost oblate-spherical to prolate-spherical, the shapes are circular in equatorial view, hexagonal in polar view, only Sonchus species are triangular or quadrangular in polar view, isopolar, symmetrical and trizonocolporate, except Sonchus and Chondrilla species, which have tri- to tetrazonocolporate pollen grains, and echinolophate. The size and number of spines in the polar area and the number of spines in the paraporal ridges and equatorial ridges proved to be the most taxonomically authentic characteristics in this study. Three pollen types were distinguished, as in alignment with previous studies. Cluster analysis by Ward method based on 10 quantitative morphological characteristics of pollen grains revealed that the species could be grouped based on the pollen characteristics. Moreover, three species of Cicerbita including Cicerbita scoparia (Rech. f. & Köie) Kitam, C. rechingeriana (Tuisl) Coșkunç., M. Güzel & N. Kilian and C. microcephala (DC.) M. Güzel, Coșkunç. & N. Kilian were recorded for the first time in Iran. Although the characteristics of the pollen grains were useful for distinguishing the species in sub-tribes, in some cases, this did not confirm recent phylogenetic results. The pollen characteristics described in this study provide data that may be useful for future systematic studies aiming to complete the knowledge on Cichorieae species. RESEARCH HIGHLIGHTS: In the present study, the pollen morphology of 33 species belonging to five genera of the Cichorieae are investigated with scanning electron microscopy and 12 species are analyzed for the first time. The cluster analysis based on the quantitative morphological characteristics of pollen grains revealed that the species could be grouped based on the pollen characteristics. In addition, three species of Cicerbita are reported for the first time in Iran.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of in vivo reflectance confocal microscopy in the diagnosis of Bowen's disease.","authors":"Shu Ding, Lingxue Hu, Yu Rao, Ruijian Ren, Xiaoliang Tong, Aiyuan Guo, Jian Huang, Zhen Tang","doi":"10.1002/jemt.24645","DOIUrl":"10.1002/jemt.24645","url":null,"abstract":"<p><p>Bowen's disease (BD) is a relatively rare early-stage squamous cell carcinoma in situ, most commonly affecting the middle-aged and elderly, and occurring on the skin or mucous membranes of various parts of the body. Its onset is concealed, the course of the disease is chronic, and some patients have malignant tumors outside the skin; therefore, it is necessary to diagnose and evaluate the disease at an early stage. This study aimed to investigate the application of reflectance confocal microscopy (RCM) in the diagnosis of BD. We performed RCM imaging on the lesion site and underwent skin biopsy for histological diagnosis of 92 patients initially diagnosed with BD in clinic. A retrospective analysis of the RCM result as well as the histological examination revealed that after analyzing RCM images, out of 92 biopsy lesions, 61 were diagnosed with BD, of which 54 were consistent with RCM diagnosis. Among the 59 cases diagnosed with BD by RCM, 54 cases were consistent with the histological diagnosis. Afterwards, we analyzed the RCM characteristics in patients with BD verified by biopsy, and compared the RCM images of two different lesions, classic Bowen's disease and pigmented Bowen's disease, and further summarized the key points of BD under RCM. Finally, we focused on the differential characteristics between BD and other skin diseases in RCM. RCM is of great value in the diagnosis of BD. RESEARCH HIGHLIGHTS: A retrospective study of RCM and histological diagnosis in patients with clinical diagnosis of BD. Analyze the RCM characteristics of skin lesions verified by biopsy. RCM is of great value in the diagnosis and differentiation of BD.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Damage regularity and multifractal analysis of sol-gel reflection coating of KDP crystal under low UV irradiation flux.","authors":"Tenghui You, Yanmeng Dai, Jian Yu, Hai-Xian Ye, Wan-Jun Dai, Lei-Feng Cao","doi":"10.1002/jemt.24652","DOIUrl":"10.1002/jemt.24652","url":null,"abstract":"<p><p>This study employed multifractal analysis to investigate the changes in surface morphology of SiO₂ anti-reflective coatings prepared on KDP substrates using the sol-gel method, under various conditions of ultraviolet (UV) irradiance. The coatings were successfully fabricated, and the chemical structure of the SiO₂ sol was comprehensively characterized using Solid-State Nuclear Magnetic Resonance (SSNMR) technology. Under low UV irradiance (4 J/cm²), repeated experiments revealed a crack-induced mechanism of surface fatigue damage. Utilizing Scanning Electron Microscopy (SEM), the study discovered the induction effect of initial crack defects in UV-damaged coatings and established a damage model. Furthermore, Atomic Force Microscopy (AFM) was used to acquire images of the coatings' surface morphology at different damage levels, which were analyzed using the multifractal spectrum f(α). This analysis confirmed the multifractal nature of the coatings both before and after damage. This study identified significant effects of UV irradiation on the width of the multifractal spectrum and Δf, indicating that the SiO₂ anti-reflective coatings exhibit multifractal characteristics under various damage states. The coatings displayed a pattern of decreasing and then increasing singularity spectrum width, height distribution unevenness, and surface roughness with increasing damage. This study demonstrates that multifractal analysis is an effective tool for describing the complexity of the surface morphology of sol-gel-derived anti-reflective coatings for the first time and for validating their multifractal properties across different stages of UV damage. HIGHLIGHTS: Damage dynamic process of KDP crystal sol-gel coating was described by SEM&AFM; The crack propagation mechanism of sol-gel coating under UV radiation is proposed; The damage evolution of sol-gel coating was described by multifractal analysis.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141731315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analyzing antimicrobial activity of ZnO/FTO, Sn-Cu-doped ZnO/FTO thin films: Production and characterizations.","authors":"Ilker Kara, Abjar Ibrahim Rashid Hafedh, Nooralhuda Kareem Hanoon Alhusseinawi, Ahmet Furkan Kayış, Özcan Yalçınkaya, Berat Cinar Acar, Zehranur Yuksekdag, Yunus Ozen, Olcay Gençyılmaz, Engin Can Ozkan, Hayrettin Oner","doi":"10.1002/jemt.24638","DOIUrl":"10.1002/jemt.24638","url":null,"abstract":"<p><p>In the developing field of nanotechnology, ZnO (zinc oxide) based semiconductor samples have emerged as the foremost choice due to their immense potential for advancing the development of cutting-edge nanodevices. Due to its excellent chemical stability, low cost, and non-toxicity to biological systems, it is also utilized in various investigations. In this study, the successive ionic layer adsorption and reaction (SILAR) method was used to generate FTO (fluorine-doped tin oxide)/ZnO, and tin (Sn)-copper (Cu)-doped ZnO thin films at varying concentrations on FTO substrates. After being stacked 40 times in varying concentrations on the FTO substrate, FTO/ZnO thin films and Sn-Cu-doped thin films were annealed at 300°C. Using Scanning Electron Microscopy (SEM) Energy Dispersive Spectroscopy-(EDS), the agar diffusion test, and the viability cell counting method, the minimum inhibitory concentration, structural properties, surface morphology, antibacterial properties, bacterial adhesion, and survival organism count of FTO/ZnO thin films and Sn-Cu-doped thin films were investigated. Both doped and FTO/ZnO films with varying Sn-Cu concentrations expanded harmonically on the FTO substrate, according to the SEM-EDS investigation. The doping concentration affected their morphological properties, causing changes depending on the doping level. Antibacterial activity was observed in the powder metals, but no antibacterial activity was found in the thin film form. The highest adhesion rate of bacterial organisms on the produced samples was observed when the FTO/ZnO/Sn-Cu doping rate was 1%. In addition, the lowest adhesion rate was observed when the FTO/ZnO/Sn-Cu additive ratio was 3%. RESEARCH HIGHLIGHTS: ZnO based semiconductors highlight significant potential in advancing nanodevice technology due to their chemical stability, cost-effectiveness, and biocompatibility. Employing the SILAR method, the study innovatively fabricates FTO/ZnO and Sn-Cu-doped ZnO thin films on FTO substrates, exploring a novel approach in semiconductor manufacturing. Post annealing at 300°C, the research examines the structural and surface morphological changes in the films, contributing to the understanding of semiconductor behavior under varying conditions. The study delves into the antibacterial properties of ZnO thin films, offering insights into the potential biomedical applications of these materials. SEM-EDS analysis reveals that doping concentrations crucially influence the morphological properties of ZnO thin films, shedding light on the optimization of semiconductor performance. Findings indicate a specific doping rate (1% Sn-Cu) enhances bacterial adhesion, while a 3% additive ratio minimizes it, suggesting implications for biomedical device engineering and antibacterial surface design.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secretory structures and histochemistry of roots, stem, and leaves of medicinal plant Fibraurea tinctoria Lour.","authors":"Septina Asih Widuri, Yohana Caecilia Sulistyaningsih, Abdul Halim Umar, Diah Ratnadewi","doi":"10.1002/jemt.24676","DOIUrl":"10.1002/jemt.24676","url":null,"abstract":"<p><p>Fibraurea tinctoria Lour. (Menispermaceae) is a medicinal plant used by many local tribes in Indonesia and other countries. This species has pharmacological potential, such as antimalaria, antibacterial, antitumor, antioxidant, anticancer, antidiabetic, anti-inflammatory, and hepato-protector. However, the secretory structures and sites of secondary metabolite accumulation still need to be studied. This study aimed to explore the secretory structures and reveal the accumulation sites of the main classes of secondary metabolites in the root, stem, and leaves of F. tinctoria. Histochemical analysis using various specific reagents was performed on freehand sections of the fresh organs to detect alkaloids, phenolics, terpenoids, and lipophilic compounds. Observations of secretory structures were carried out using light microscopy. The result showed that idioblast was the predominant secretory structure in the plant organs and contained all the chemical groups investigated, while laticifer accumulated alkaloids were found only in the stem. Some secondary metabolites were localized in the xylem, intercellular space, and cuticle layer. Crystals were observed in the root, stem, and leaf of this species. These results might be helpful as guide information for extracting the particular plant parts to obtain the desired secondary metabolites. It suggested that leaves are a potential alternative source of medicinal raw material of this species, in addition to the stem. RESEARCH HIGHLIGHTS: The first study on secretory structures of Fibraurea tinctoria. Secondary metabolites in F. tinctoria organs accumulate in specialized and unspecialized structures. The idioblast is the primary secretory structure in the organs of F. tinctoria spreading over the root, stem, and leaves.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141909986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling thrips morphology: A comparative analysis of microscopy-based ultrastructural, morphological, and molecular characterization of Thrips tabaci and Thrips parvispinus in onion.","authors":"Arunkumar Pandi, Kannan Malaichamy, Kennedy John Samuel, Dineshkumar Selvaraj, Govindaraju Kasivelu, Elango Kolanthasamy","doi":"10.1002/jemt.24653","DOIUrl":"10.1002/jemt.24653","url":null,"abstract":"<p><p>The present study unveils the intricate details on the morphology of thrips through optical, field emission scanning electron microscopy (FE-SEM) and mitochondrial cytochrome oxidase I gene-based molecular identification tools. The variation in the morphological characters namely, antennae (seven-segmented with forked sensorium on third, fourth segments), ctenidia (paired ctenidia were present in 5^th-8^th abdominal segments laterally), pronotum (two pairs of posteroangular setae) were observed in both Thrips tabaci and Thrips parvispinus, respectively. Similarly, ocelli color (brown and red colored), ocellar setae (two and three pairs of ocellar setae on the head of T. tabaci and T. parvispinus, respectively. Irregular reticulate striations on metascutum and medial striations are present in the metanotum of T. parvispinus; forewings with 6 distal setae in the first vein and 15 distal setae in the second vein in T. tabaci and forewings of T. parvispinus with complete rows of setae in the first and second vein in T. parvispinus; abdomen with median dorsal setae present in the tergite of T. tabaci and presence of 6-12 discal setae in sternites III-VI segments, absence of discal setae on sternites II and VII in T. parvispinus were observed, respectively. Further, FE-SEM studies revealed that similar type of sensilla namely, sensilla basiconica (SBI, SBII, SBIII), sensilla chaetica (SChI, SChII), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav) were recorded in both the species and variations were observed in length of above sensilla of T. tabaci and T. parvispinus. Additionally, Bohm bristles (Bb) and microtrichia (Mt) on the antennal surface contributed to a comprehensive understanding of their ultrastructural features. The molecular characterization revealed a single ~450 bp nucleotide fragment with over 98% similarity for the confirmation of T. tabaci and T. parvispinus in concurrence with NCBI data. RESEARCH HIGHLIGHTS: Microscopy-based morphological and ultrastructural characterization of Thrips tabaci Lindeman and Thrips parvispinus Karny.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the potential impact of sodium-glucose cotransporter-2 inhibitor \"canagliflozin\" on the hepatic damage triggered by hypertension in rats.","authors":"Fatma E Hassan, Aliaa E M K El-Mosallamy, Mohamed Mansour Khalifa, Samira H Aljuaydi, Merhan E Ali, Sara Adel Hosny, Nermeen Bastawy","doi":"10.1002/jemt.24665","DOIUrl":"10.1002/jemt.24665","url":null,"abstract":"<p><p>Hypertension (HTN) is a prevalent chronic disease. HTN and liver disease association is extensively noted. Thus, finding a medication that can alleviate HTN and its accompanying liver insult would be promising. This study investigated the potential impacts of canagliflozin \"sodium-glucose cotransporter-2 inhibitor\" on the liver of the N^ω-nitro-L-arginine methyl ester (L-NAME)-induced HTN rat model. Twenty-four adult male rats were divided into four groups; negative control group, canagliflozin group, L-NAME group: 50 mg/kg of L-NAME was injected daily for 5 weeks and L-NAME + canagliflozin group: 1 week after L-NAME injection both L-NAME + canagliflozin (40 mg/kg) were given concomitantly daily for further 4 weeks. Liver functions, serum lipid profile, hepatic oxidative/nitrative stress biomarkers, gene expression of lipogenic enzymes, B-cell lymphoma 2 (Bcl2), and DNA fragmentation, were measured. Besides, hepatic histology and immunohistochemistry of nuclear factor kappa B (NF-κB) and endothelial nitric oxide synthase (eNOS) were assessed. Canagliflozin improved hepatic lipogenesis via the downregulation of fatty acid synthase (FAS) and transcriptional regulatory element binding protein 1c (SREBP1c) genes leading to an improved serum lipid profile. Further, canagliflozin modified the eNOS/inducible nitric oxide synthase (iNOS) pathway and decreased the NF-κB immunoreactivity besides restoring the oxidants-antioxidants balance; increased reduced glutathione concomitant with declined malondialdehyde. This improvement of the liver was mirrored by the significant restoration of liver architecture and confirmed by the preserved liver DNA content and upregulation of the antiapoptotic Bcl2 mRNA level and attenuation of the alanine transaminase, aspartate aminotransferase. In conclusion, canagliflozin is a promising anti-hypertensive and hepatic-supportive medication. RESEARCH HIGHLIGHTS: Canagliflozin's antioxidant, anti-inflammatory, anti-lipogenic, and antiapoptotic characteristics mitigate remote liver compromise caused by hypertension. Canagliflozin can be exploited as a hepatoprotective and antihypertensive medication.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breast histopathological imaging using ultra-fast fluorescence confocal microscopy to identify cancer lesions at early stage.","authors":"Muhammad Mujahid, Amjad Rehman Khan, Mahyar Kolivand, Faten S Alamri, Tanzila Saba, Saeed Ali Omer Bahaj","doi":"10.1002/jemt.24677","DOIUrl":"10.1002/jemt.24677","url":null,"abstract":"<p><p>Ultrafast fluorescent confocal microscopy is a hypothetical approach for breast cancer detection because of its potential to achieve instantaneous, high-resolution images of cellular-level tissue features. Traditional approaches such as mammography and biopsy are laborious, invasive, and inefficient; confocal microscopy offers many benefits over these approaches. However, confocal microscopy enables the exact differentiation of malignant cells, the expeditious examination of extensive tissue sections, and the optical sectioning of tissue samples into tiny slices. The primary goal should be to prevent cancer altogether, although detecting it early can help achieve that objective. This research presents a novel Breast Histopathology Convolutional Neural Network (BHCNN) for feature extraction and recursive feature elimination method for selecting the most significant features. The proposed approach utilizes full slide images to identify tissue in regions affected by invasive ductal carcinoma. In addition, a transfer learning approach is employed to enhance the performance and accuracy of the models in detecting breast cancer, while also reducing computation time by modifying the final layer of the proposed model. The results showed that the BHCNN model outperformed other models in terms of accuracy, achieving a testing accuracy of 98.42% and a training accuracy of 99.94%. The confusion matrix results show that the IDC positive (+) class achieved 97.44% accuracy and 2.56% inaccurate results, while the IDC negative (-) class achieved 98.73% accuracy and 1.27% inaccurate results. Furthermore, the model achieved less than 0.05 validation loss. RESEARCH HIGHLIGHTS: The objective is to develop an innovative framework using ultra-fast fluorescence confocal microscopy, particularly for the challenging problem of breast cancer diagnosis. This framework will extract essential features from microscopy and employ a gradient recurrent unit for detection. The proposed research offers significant potential in enhancing medical imaging through the provision of a reliable and resilient system for precise diagnosis of breast cancer, thereby propelling the progression of state-of-the-art medical technology. The most suitable feature was determined using BHRFE optimization techniques after retrieving the features by proposed model. Finally, the features chosen are integrated into a proposed methodology, which is then classified using a GRU deep model. The aforementioned research has significant potential to improve medical imaging by providing a complex and reliable system for precise evaluation of breast cancer, hence advancing the development of cutting-edge medical technology.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-molecular analyses and scanning electron microscopy reveal two new species of genus Lactarius from the northwestern Himalaya, India.","authors":"Komal Verma, Shiny Singh, Priyanka Uniyal, Tahir Mehmood, Yash Pal Sharma","doi":"10.1002/jemt.24661","DOIUrl":"10.1002/jemt.24661","url":null,"abstract":"<p><p>One of the largest subgenera of the genus Lactarius, Lactarius subg. Russularia is abundant in the forests of the Himalayas. This article proposes Lactarius crassostipes sp. nov. and Lactarius flavocorpus sp. nov. under the L. subgenus Russularia. It includes comprehensive macro- and micro-morphological descriptions, scanning electron microscopy (SEM) micrographs, drawings, and comparisons with related species. The taxa's novelty is validated using phylogenetic analyses based on nrITS. RESEARCH HIGHLIGHTS: SEM and subsequent nrITS-based molecular phylogeny revealed two novel species (L. crassostipes and L. flavocorpus) of the genus Lactarius subg. Russularia. To further characterize the size, form, and ornamentation of these novel species' basidiospores, SEM analysis is an additional tool. In L. crassostipes \"crasso\" means thick and \"stipes\" indicates stipe. In contrast to L. flavocorpus, where \"flavocorpus\" refers to the golden yellow fruiting body, the name alludes to its unique thick stipe. L. crassostipes sp. nov. and L. flavocorpus sp. nov have been collected from the coniferous and broad-leaved forests of Union Territory of Jammu and Kashmir, India, respectively. The diagnostic characteristics, habit, habitat, and similarities to related species are given.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}