Microscopy Research and Technique最新文献_第2页

Polypropylene Mesh Enhanced Healing of Large Metacarpal Wounds in Donkeys: Gross and Histopathological Insights. 聚丙烯网增强愈合大掌骨伤口驴：肉眼和组织病理学的见解。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-06-30 DOI: 10.1002/jemt.70020

Osama Eissa, Ahmed F Ahmed, Ramy K A Sayed, Mohamed A Semieka, Nashwa Hamad

{"title":"Polypropylene Mesh Enhanced Healing of Large Metacarpal Wounds in Donkeys: Gross and Histopathological Insights.","authors":"Osama Eissa, Ahmed F Ahmed, Ramy K A Sayed, Mohamed A Semieka, Nashwa Hamad","doi":"10.1002/jemt.70020","DOIUrl":"10.1002/jemt.70020","url":null,"abstract":"Wounds of the distal limb in equines are restored after a long period with the development of excessive granulation tissues and poor wound contraction and re-epithelialization. Although, the usage of mesh implantation has been emerged in hernioplasty in animals and humans, its effects on treatment of distal limb wounds in equines are still undefined. Hence, this study was intended to inspect the impact of polypropylene mesh application on healing of large-sized metacarpal skin wounds in donkeys through gross and histopathological investigations on days 28, 56, and 84 post-wounds induction. The results revealed that granulation tissues formed over the outer surface of the polypropylene mesh and emerged from the pores of the polypropylene mesh. Re-epithelialization from the four sides of the wound in addition to wound contraction induced a significant reduction in the wound size compared to the control group. Histopathological results revealed that the healing process was superior in mesh-implanted wounds through promoting re-epithelialization, neovascularization, fibroblast proliferation, collagen maturation, and reduced scar formation. In conclusion, the polypropylene mesh was beneficial in accelerating the healing process through enhancing epithelialization, properly limiting and regulating exaggerated hyper granulation tissues, and promoting the formation of healthy skin suggesting its promising application for metacarpal skin wounds of donkeys.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2886-2897"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Nonlinear Optical Microscopy: Shedding Light on Oral and Dental Tissues. 无标签非线性光学显微镜：在口腔和牙齿组织上发光。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-07-09 DOI: 10.1002/jemt.70012

Tarcília Aparecida Silva, José Alcides Almeida de Arruda, Nayara Conceição Marcos Santana, Renan Souza Cunha, Victor Zanetti Drumond, Leandro Malard Moreira, Bruno Augusto Benevenuto de Andrade, Juliane Isaac, Nicolas Dupré, Benjamin P J Fournier

{"title":"Label-Free Nonlinear Optical Microscopy: Shedding Light on Oral and Dental Tissues.","authors":"Tarcília Aparecida Silva, José Alcides Almeida de Arruda, Nayara Conceição Marcos Santana, Renan Souza Cunha, Victor Zanetti Drumond, Leandro Malard Moreira, Bruno Augusto Benevenuto de Andrade, Juliane Isaac, Nicolas Dupré, Benjamin P J Fournier","doi":"10.1002/jemt.70012","DOIUrl":"10.1002/jemt.70012","url":null,"abstract":"Nonlinear optical microscopy (NLOM) enables 3D imaging with submicron resolution, and autofluorescence spectro-microscopy of oral and dental tissues shows promise for enhancing the analysis of both healthy and diseased tissues. We critically reviewed studies involving humans and animals, examining oral and dental tissues in vivo and ex vivo using label-free NLOM. A scientometric analysis was also conducted to assess the 25-year trajectory of NLOM in dentistry. A total of 78 studies from 17 countries were evaluated. Second harmonic generation (SHG) was the most commonly employed technique (83.3%), often combined with other NLOM methods (68%). The primary specimens imaged were the oral mucosa and teeth, with at least 70% of the studies contributing new morphological or biochemical insights and 34.6% generating data with potential clinical applications. SHG and cancer diagnosis were prominent descriptors, reflecting key findings from the most-cited articles. Data indicate that NLOM provides robust and reliable clinical information, particularly for cancer and dental caries diagnosis; however, challenges remain in translating this knowledge into practice. Integrating this technology into clinical devices could advance dentistry by creating noninvasive tools for long-term monitoring with minimal intervention and sample preparation.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"3050-3062"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lithium Disilicate Ceramics Pretreated With Ti: Sapphire fs, Er; Cr: YSGG, and Er: YAG Lasers: A Study on Mechanical Properties, Energy Dispersive Spectroscopy, Color Alterations, and Surface Topography. Ti：蓝宝石fs， Er预处理二硅酸锂陶瓷Cr: YSGG和Er: YAG激光器：力学性能、能量色散光谱、颜色变化和表面形貌的研究。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-07-07 DOI: 10.1002/jemt.70032

Saud Ali Alqahtani, Ali Y Alsaeed, Saeed M Alqahtani, Shaheryar Shafqat, Muhammad Omar Niaz, Yusra Ishtiaque, Eisha Abrar

{"title":"Lithium Disilicate Ceramics Pretreated With Ti: Sapphire fs, Er; Cr: YSGG, and Er: YAG Lasers: A Study on Mechanical Properties, Energy Dispersive Spectroscopy, Color Alterations, and Surface Topography.","authors":"Saud Ali Alqahtani, Ali Y Alsaeed, Saeed M Alqahtani, Shaheryar Shafqat, Muhammad Omar Niaz, Yusra Ishtiaque, Eisha Abrar","doi":"10.1002/jemt.70032","DOIUrl":"10.1002/jemt.70032","url":null,"abstract":"To assess the efficiency of Er; Cr: YSGG laser (ECL), Er: YAG laser (EYL), and the Ti: sapphire femtosecond laser (TsFL) in terms of color change (ΔE) measured by a spectrophotometer, surface topography observed through SEM, surface roughness (Ra), and bond strength of Lithium disilicate glass-ceramic (LDC) to resin cement. A total of 84 LDC disks were manufactured and assigned randomly into four groups. Group 1-HFA treated, Group 2 treated with TsFL, Group 3 with EYL, and Group 4 with ECL. Each disk was coated with a silane coupling agent. Surface profiling of the LDC disc was evaluated via SEM and Ra was measured using a profilometer. The ΔE values were determined using the CIE L*a*b* system. Adhesive luting cement was applied to 10 samples from each group, followed by thermocycling to simulate aging. Shear bond strength (SBS) was assessed, and failure modes were inspected following SBS. ANOVA and Tukey's post hoc test were used for the comparison of means and SD among groups. The highest ΔE was observed in the control HFA (4.23 ± 1.02) treated group. Whereas, Group 2 TsFL (2.22 ± 1.11) pretreated samples displayed the lowest ΔE. Ra and SBS analysis unveiled that the maximum score of Ra (1155.01 ± 1.43 μm) and SBS (10.28 ± 0.17 MPa) was observed in Group 2 TsFL. Group 4 (ECL) conditioned discs showed the lowest Ra (1015.41 ± 1.31 μm) and bond integrity (8.15 ± 0.16 MPa). The TsFL serves as an effective alternative to traditional surface preparation methods as it enhances the mechanical properties of LDC (SBS and Ra) with no susceptible change in ΔE.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"3029-3036"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine Learning Based Multi-Class Classification and Grading of Squamous Cell Carcinoma in Optical Microscopy. 光学显微镜下基于机器学习的鳞状细胞癌多类分类与分级。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-06-27 DOI: 10.1002/jemt.70016

Sindhoora Kaniyala Melanthota, Spandana K U, Raghavendra U, Sharada Rai, Rakshatha Nayak, Yuri V Kistenev, Suranjan Shil, K K Mahato, Nirmal Mazumder

{"title":"Machine Learning Based Multi-Class Classification and Grading of Squamous Cell Carcinoma in Optical Microscopy.","authors":"Sindhoora Kaniyala Melanthota, Spandana K U, Raghavendra U, Sharada Rai, Rakshatha Nayak, Yuri V Kistenev, Suranjan Shil, K K Mahato, Nirmal Mazumder","doi":"10.1002/jemt.70016","DOIUrl":"10.1002/jemt.70016","url":null,"abstract":"Histopathological tissue grading is critical for disease diagnosis and treatment, but manual grading is labor-intensive and time-consuming, requiring expert pathologists. This study presents an efficient analysis of squamous cell carcinoma (SCC) histopathological images using machine learning (ML) and deep learning (DL) models. Five ML models-support vector machine, Naïve Bayes, decision tree, k-nearest neighbor (KNN), and neural network-were trained with 5-, 7-, and 10-fold cross-validation. Discrete wavelet transform along with gray level co-occurrence matrix and histogram features extracted 360 features per image, and Student's t-test selected 114 key features. Among ML models, KNN with sevenfold cross-validation achieved 98% accuracy. Additionally, a convolutional neural network (CNN) trained achieved 98.23% accuracy in automated classification. These results suggest that combining ML for feature analysis with interpretable DL models can lead to more accurate and efficient SCC grading, reducing reliance on manual pathology.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2865-2877"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Different Concentrations of Bismuth Molybdate as an Electrocatalyst for Water Splitting and Antibacterial Activity. 不同浓度钼酸铋电催化剂对水裂解及抗菌活性的影响。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-07-07 DOI: 10.1002/jemt.70022

Toaqeer Salman, Mohsin Javed, Sumera Zaib, Ahmad Alhujaily, Muhammad Adil Mansoor, Hamid Saeed Shah, Madiha Khan, Imtiaz Khan, Shahid Iqbal, Shaimaa A M Abdelmohsen, Randa A Althobiti

{"title":"Effects of Different Concentrations of Bismuth Molybdate as an Electrocatalyst for Water Splitting and Antibacterial Activity.","authors":"Toaqeer Salman, Mohsin Javed, Sumera Zaib, Ahmad Alhujaily, Muhammad Adil Mansoor, Hamid Saeed Shah, Madiha Khan, Imtiaz Khan, Shahid Iqbal, Shaimaa A M Abdelmohsen, Randa A Althobiti","doi":"10.1002/jemt.70022","DOIUrl":"10.1002/jemt.70022","url":null,"abstract":"In this work, five different molar concentrations of Bi2MoO6 are prepared using the hydrothermal method. SEM, XRD, and FTIR are used to find out the morphology of the synthesized materials as ratio 1 (1:1), 2 (0.8:1.2), 3 (1.2:0.8), 4 (0.6:1.4), and 5 (1.4:0.6). The newly formed nanomaterial is spherical to cubic in shape as per the SEM results, with some agglomerates. The water splitting and antibacterial activity of the electrocatalyst are carried out without any irradiation of light, which is the novelty of this research work. According to EIS (parameter of water splitting), the change transfer (Rct) value for ratio 3 (1.2:0.8) is 5 Ω and the Rct value for ratio 5 (1.4:0.6) is 8.48 Ω. The Rs value for ratio 1 (1:1) was very low, Rs = 5.77 Ω, compared to the Rs value for ratio 2 (0.8:1.2), Rs = 6.75 Ω. For instance, activity for ratio 1 (1:1) showed less overpotential for HER and OER, which led to more water splitting and a lower Rct number. For a better understanding, all the electrochemical factors and the time sequence of the different Bi2MoO6 ratios should be looked at: 1:1 ratio > 0.8:1.2 ratio > 1.2:0.8 ratio > 0.6:1.4 ratio > 1.4:0.6 ratio. The experimental results suggest that a 1:1 ratio between Bi and Mo exhibits higher electrochemical as well as antibacterial activity results.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"3017-3028"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Edible Bird's Nest Soup (EBNS) Attenuates Glomerular Basement Membrane Thickening and Tubular NF-κβ and NLRP3 Expression in the Acute Renal Injuries Linked to Obesity in Rats. 食用燕窝汤（EBNS）减轻肥胖急性肾损伤大鼠肾小球基底膜增厚及肾小管NF-κβ和NLRP3的表达。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-07-02 DOI: 10.1002/jemt.70018

Mohd Noor Akmal, Abdul Razak Intan-Shameha, Zuki Abu Bakar, Mokrish Ajat

{"title":"Edible Bird's Nest Soup (EBNS) Attenuates Glomerular Basement Membrane Thickening and Tubular NF-κβ and NLRP3 Expression in the Acute Renal Injuries Linked to Obesity in Rats.","authors":"Mohd Noor Akmal, Abdul Razak Intan-Shameha, Zuki Abu Bakar, Mokrish Ajat","doi":"10.1002/jemt.70018","DOIUrl":"10.1002/jemt.70018","url":null,"abstract":"This study aimed to elucidate how edible bird's nest soup (EBNS) and edible bird's nest extract (EBNE) can protect the kidneys of obese rats from acute kidney injuries caused by a high-fat diet (HFD) via histological techniques. A total of 24 male Sprague-Dawley rats aged 12 weeks old were utilized in this study. The rats were randomly assigned (n = 6) to either the chow diet; HFD; HFD + EBNS (843.2 mg/kg); or HFD + EBNE (6.5 mg/kg) for 12 weeks. Body mass gain and visceral adipose tissue (VAT) were measured, and the kidneys were collected for histopathological analysis, including H&E, PAS staining, and immunohistochemistry (TLR4, NF-κβ and NLRP3). Results showed the EBNS group maintained normal body mass (530.19 ± 0.32) via the reduction of VAT (28.79 ± 0.24) influx, similar as in the BC group (527.78 ± 0.77; 30.61 ± 0.91). Meanwhile, the EBNE group demonstrated significant body mass gain (597.04 ± 0.52) and VAT (45.03 ± 0.38) deposition. The glomeruli diameters (104.76 ± 2.77) in the EBNS group were normal compared to those in the EBNE group (142.05 ± 1.51), which showed glomerulomegaly and membranous glomerulonephritis. In the EBNE group, the NF-κβ and NLRP3's immunoreactivity covered as much as 44.38% ± 0.21% and 60.77% ± 0.53%, respectively, in the tissue section. Meanwhile, the EBNS group observed only 2.85% ± 0.82% (NF-κβ) and 6.80% ± 0.62% (NLRP3) immunoreactivity in the tissue. These findings were consistent with mild (1.83 ± 0.70) and moderate (5.65 ± 0.30) tubular inflammatory cell infiltration in the EBNS and EBNE groups, respectively. In the obese rat model induced by HFD, the EBNS was able to prevent the obesity-related acute kidney injuries by maintaining a normal architecture of glomerular histomorphology and suppressing the tubular NF-κβ and NLRP3 expression.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2977-2988"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144553988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning Automated Measurements of Expanded Polystyrene Beads Size Using Low-Resolution Micrography. 深度学习自动测量膨胀聚苯乙烯珠尺寸使用低分辨率显微摄影。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-07-04 DOI: 10.1002/jemt.70019

Alejandro E Rodríguez-Sánchez, Héctor Plascencia-Mora

{"title":"Deep Learning Automated Measurements of Expanded Polystyrene Beads Size Using Low-Resolution Micrography.","authors":"Alejandro E Rodríguez-Sánchez, Héctor Plascencia-Mora","doi":"10.1002/jemt.70019","DOIUrl":"10.1002/jemt.70019","url":null,"abstract":"The analysis of microscopic characteristics of closed-cell polymeric foams, particularly bead size, is relevant for understanding properties such as thermal insulation, energy absorption, and compressive structural strength of these materials. This study presents an automated method based on Deep Learning models to measure the bead size of Expanded Polystyrene foams in low-resolution micrographs. The results of this approach were compared with manual measurements at two expanded polystyrene foam densities: 8.5 and 24 kg/m3. Hypothesis tests, including Student's t-test, Levene's test, and Mann-Whitney U test, were conducted and showed no significant differences between manual and automatic measurements. Student's t-test and Levene's test indicated that both methods have comparable means and variances, while the Two One-Sided Test confirmed that they were equivalent for bead size measurement. Additionally, the Mann-Whitney U test revealed no differences in medians, and Bland-Altman plot analyses demonstrated no systematic bias between the methods. Taken together, these results suggest that the proposed Deep Learning-based method is a reliable and precise substitute for the manual method in measuring the bead size of expanded polystyrene, making it suitable for practical use in the bead microstructural analysis of expanded polystyrene material.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2999-3008"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of Surface Deformation in NiTi Endodontic Reciprocating Instruments Manufactured by Machining. 机械加工镍钛根管往复器械表面变形的测量。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-06-30 DOI: 10.1002/jemt.70023

Yenny Marcela Orozco-Ocampo, César Augusto Álvarez Vargas, Francy Nelly Jiménez-García, Daniel Escobar-Rincón, Paola Ximena Jaramillo-Gil

{"title":"Measurement of Surface Deformation in NiTi Endodontic Reciprocating Instruments Manufactured by Machining.","authors":"Yenny Marcela Orozco-Ocampo, César Augusto Álvarez Vargas, Francy Nelly Jiménez-García, Daniel Escobar-Rincón, Paola Ximena Jaramillo-Gil","doi":"10.1002/jemt.70023","DOIUrl":"10.1002/jemt.70023","url":null,"abstract":"This study aimed to develop a methodology to quantify the surface deformation of WaveOne-Gold-Primary instruments after in vitro use. Fifteen reciprocating instruments were used to prepare artificial canals composed of diallyl-phthalate, bisphenol-A, and duralumin (n = 5 per group). Optical microscopy was employed to determine Euler-Almansi strains, scanning electron microscopy (SEM) was used to identify shear bands and characterize fracture surfaces, and energy-dispersive X-ray spectroscopy (EDS) was used to analyze elemental composition. An ANOVA (95%) was conducted to assess significant differences in the strains. The instruments used in polymer canals exhibited untwisting and pronounced elongation, indicative of plastic deformation associated with ductile fractures. Although, atomic percentages of Ni and Ti remained relatively stable, a slight reduction in Ni was observed in the coating of the sterilized instruments. A mathematical model was proposed to correlate the strains with the number of pecks. This methodology provides a robust framework for measuring the deformation of machined NiTi instruments, contributing to a better understanding of their failure and potential prediction implications.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2898-2908"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Different Sealer Placement and Activation Techniques on Sealer Penetration Depth and Penetration Area. 不同封口剂放置和活化技术对封口剂渗透深度和渗透面积的影响。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-06-30 DOI: 10.1002/jemt.70007

Emrah Karataşlioğlu, Samet Tosun

{"title":"Effect of Different Sealer Placement and Activation Techniques on Sealer Penetration Depth and Penetration Area.","authors":"Emrah Karataşlioğlu, Samet Tosun","doi":"10.1002/jemt.70007","DOIUrl":"10.1002/jemt.70007","url":null,"abstract":"This study evaluated the effect of different activation methods (no activation, sonic, ultrasonic, and XP-Endo Finisher) on the penetration depth and area of AH Plus and MTA Fillapex sealers. Two hundred single-rooted teeth were prepared and randomly divided into eight groups (n = 25). Sealers stained with rhodamine B were inserted and activated as follows: Group 1 (control, no activation, G1A, G1M), Group 2 (sonic: G2A, G2M), Group 3 (ultrasonic: G3A, G3M), and Group 4 (XP-Endo Finisher: G4A, G4M). The single cone technique was used for filling, and specimens were sectioned perpendicularly. Confocal laser scanning microscopy (CLSM) measured penetration depth, while penetration area was calculated as the sealer's penetration relative to the canal area. Two-way ANOVA was used for statistical analysis. Ultrasonic and XP-Endo Finisher activation significantly increased the penetration depth of MTA Fillapex compared to the control group (p < 0.05), while no significant difference was found for AH Plus (p > 0.05). MTA Fillapex also demonstrated a significantly greater penetration area than AH Plus when ultrasonic and XP-Endo Finisher activation were applied (p < 0.05). No significant differences were observed between sealers in the control and sonic groups (p > 0.05). Activation methods did not significantly affect the penetration area of AH Plus (p > 0.05). XP-Endo Finisher and ultrasonic activation significantly improved both penetration depth and area for MTA Fillapex but had limited effect on AH Plus. These findings highlight the clinical importance of selecting the appropriate activation technique based on the type of sealer used, as enhanced penetration may contribute to improved sealing ability and long-term endodontic success.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2878-2885"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of Sol-Gel Assisted TiO₂ Nanostructures for Photocatalytic Degradation of Methylene Blue. 光催化降解亚甲基蓝的溶胶-凝胶辅助TiO2纳米结构的合成

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-11-01 Epub Date: 2025-07-04 DOI: 10.1002/jemt.70002

Aqsa Bibi, Muhammad Hafeez, Saleem Raza, Muhammad Usman Hameed, Jahanzeb Khan, Muhammad Zaheer

{"title":"Synthesis of Sol-Gel Assisted TiO2 Nanostructures for Photocatalytic Degradation of Methylene Blue.","authors":"Aqsa Bibi, Muhammad Hafeez, Saleem Raza, Muhammad Usman Hameed, Jahanzeb Khan, Muhammad Zaheer","doi":"10.1002/jemt.70002","DOIUrl":"10.1002/jemt.70002","url":null,"abstract":"It is highly needed for future generations to develop a low-cost photocatalysts for the degradation of colored dyes to save our environment. Herein, we report an efficient and low-cost sol-gel method for the synthesis of titanium dioxide (TiO2) nanostructures. The structural, compositional, optical, and morphological properties of the as-synthesized samples have been analyzed by various analytical techniques, including X-ray Diffraction, Fourier Transform Infrared Spectroscopy (FTIR), Ultraviolet-Visible Spectroscopy (UV-Vis), Energy-Dispersive X-ray Spectroscopy (EDX), and Scanning Electron Microscope (SEM). X-ray Diffraction analysis identified distinct anatase peaks, with the rutile phase emerging only at 900°C, along with increased crystal size and lattice strain. SEM images showed morphological changes, transitioning to spherical nanobeads at higher calcination temperatures, forming either dispersed or clustered structures. FTIR spectra revealed temperature-dependent changes in surface chemistry, indicating the presence of various functional groups. UV-Vis spectroscopy indicated shifts in λmax values, reflecting changes in electronic structure and crystallinity, particularly a shift to 334 nm at 900°C, signaling a significant transformation in electronic structure. EDX analysis confirmed the presence of TiO2 with minimal impurities. The photocatalytic activity was evaluated by degrading methylene blue under visible light, revealing how TiO2 properties can be fine-tuned through parameters such as calcination temperature, time, pH, and concentration ratio. Remarkably, TiO2 calcined at 900°C with 6% concentration revealed nearly complete Methylene Blue degradation after 180 min, highlighting its superior performance among the tested catalysts. This work will deliver a possible route to the synthesis of efficient and low-cost photocatalysts to develop for environmental remediation.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":"2989-2998"},"PeriodicalIF":2.1,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144560455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0