Microscopy Research and Technique最新文献

{"title":"Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.","authors":"Carolina Sabedotti, João Vitor da Costa Batista, Bettina Leonhard, Konrad Urech, Mahsa Ahmadinezhad, Jakob Maier, Stephan Baumgartner, Fabio Boylan, Carla Holandino, Jane Manfron","doi":"10.1002/jemt.70153","DOIUrl":"https://doi.org/10.1002/jemt.70153","url":null,"abstract":"<p><p>Primulaceae are distributed all over the world, encompassing around 2590 species. The genus Lysimachia is also part of this family. Lysimachia nummularia L., popularly known as moneywort or creeping Jenny, has been used in Europe to treat many diseases. However, there are no reports of a complete morpho-anatomical description for this species. The aim of this study was to characterize the morpho-anatomical features of the plant, as well as to establish High-Performance Thin-Layer Chromatography (HPTLC)-fingerprints of extracts prepared from the plant by decoction and infusion as well as the organic fractions obtained from these extracts. Standard techniques of light and scanning electron microscopy, as well as histochemical tests, were used; a standard method of HPTLC was applied. The species shows simple, opposite, membranaceous leaves, with rounded roots containing a central medulla and a ribbed stem with endoderm. Amphistomatic leaves bear glandular trichomes on the abaxial surface, also found on the petiole, midrib, petal, and filament. Histochemical analyses confirmed the presence of lipophilic compounds, proteins, starch, phenolics, tannins, and lignin. HPTLC analysis showed some differences in the fingerprints of the organic fractions examined, yet it highlighted the need for further studies regarding the chemical composition of the plant.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).","authors":"K Logeswaran, Suresh R Jambagi, Vinithashri Gautam, T Venkatesan, D Sagar, S N Sushil, M Mohan","doi":"10.1002/jemt.70154","DOIUrl":"https://doi.org/10.1002/jemt.70154","url":null,"abstract":"<p><p>The pink bollworm, Pectinophora gossypiella Saunders is a major pest of cotton, notorious for its high reproductive potential and rapid evolution of resistance to Bacillus thuringiensis (Bt) toxins. Despite its economic significance, detailed knowledge of its reproductive anatomy and egg ultrastructure has remained limited, constraining the development of advanced molecular control strategies such as CRISPR/Cas9-based genome editing. The present study provides the first comprehensive characterization of the reproductive system and egg surface morphology of P. gossypiella using stereomicroscopy and scanning electron microscopy (SEM) techniques. The male reproductive system consists of fused, bean-shaped testes, seminal vesicles, duplex and simplex ejaculatory ducts, and paired accessory glands. The female reproductive system comprises paired ovaries with four polytrophic ovarioles per ovary, lateral and common oviducts, accessory glands, corpus bursae, and spermathecal glands. Eggs are oval, dorsoventrally flattened, exhibit a reticulated chorion with distinct micropylar and aeropylar regions. SEM images revealed 6-9 rosette cells encircling a circular micropylar plate, 14-19 first order and 17-23 s order ribs, and 250-291 polygonal surface cells. The structural features of P. gossypiella eggs reveal key sites for sperm entry, aeropylar respiration, and candidate zones for microinjection in gene editing applications. These findings establish a morphological baseline critical for optimizing embryo manipulation and ribonucleoprotein (RNP) delivery in lepidopteran genome editing. This study represents a pioneering effort to integrate classical egg morphology with molecular entomology, thereby advancing precision genetic interventions aimed at resistance management and population suppression in P. gossypiella.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated FRET Analysis for Enhanced Characterization of Protein-Protein Interactions.","authors":"Ahmet Zübeyir Nursoy, Orkun Cevheroğlu, Çağdaş Devrim Son","doi":"10.1002/jemt.70147","DOIUrl":"https://doi.org/10.1002/jemt.70147","url":null,"abstract":"<p><p>Förster Resonance Energy Transfer (FRET) analysis is a powerful technique for studying protein-protein interactions; however, manual methods often introduce variability and user dependency. We present the SONLab FRET Analysis Tool, an open-source and automated software that integrates Cellpose for cell segmentation with standardized pipelines for bleed-through correction and FRET efficiency calculation. By minimizing human intervention, the tool improves reproducibility and comparability between experiments. The results demonstrate that the tool achieves FRET efficiencies comparable to those of manual methods but with reduced bias, enabling robust and high-throughput analysis of protein interactions.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTION: Effect of Encapsulation or Grafting on Release Kinetics of Recombinant Human Bone Morphogenetic Protein-2 from Self-Assembled Poly(Lactide-co-Glycolide Ethylene Oxide Fumarate) Nanoparticles.","authors":"","doi":"10.1002/jemt.70150","DOIUrl":"https://doi.org/10.1002/jemt.70150","url":null,"abstract":"<p><strong>Retraction: </strong>A. E. Mercado and E. Jabbari, \"Effect of Encapsulation or Grafting on Release Kinetics of Recombinant Human Bone Morphogenetic Protein-2 from Self-Assembled Poly(Lactide-co-Glycolide Ethylene Oxide Fumarate) Nanoparticles,\" Microscopy Research and Technique 73, no. 9 (2010): 824-833, https://doi.org/10.1002/jemt.20846. The above article, published online on 23 August 2010 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editors-in-Chief, Julia Fernandez-Rodriguez and Paula Sampaio; and Wiley Periodicals LLC. The retraction has been agreed upon following concerns raised by a third party. An investigation identified duplication of the inset image included in Figure 4 with another previously published article, as well as duplication of Figures 6A and 6B in three other earlier articles by overlapping authors, without disclosure of reuse. Furthermore, the images in Figures 6A and 6B are described differently in some of the earlier articles, raising concerns affecting the interpretation of the results. The editors consider the results and conclusions to be unreliable. The authors disagree with the retraction.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Morphological Study of Restoration of Damaged Hair Fiber With Chemical Treatments Using Microscopy Techniques.","authors":"L P A Mauricio, R A D Cunha, S M Alves","doi":"10.1002/jemt.70149","DOIUrl":"https://doi.org/10.1002/jemt.70149","url":null,"abstract":"<p><p>This paper analyses hair restoration products currently on the market that treat chemically processed hair. This study measured the restoration of structural integrity in hair samples from both chemically damaged hair (chemically relaxed, bleached, and chemically color-treated) and virgin hair. Hair samples were exposed to two hair restoration products (low- and high-cost), formulated with ingredients from two different price points. This analysis is essential because of the challenges in finding scientific information on treatment protocols for processes that can damage hair. The effectiveness of these treatments was evaluated by assessing the structural damage caused by the chemical processes using scanning electron microscopy (SEM), atomic force microscopy (AFM), and Raman spectroscopy. The results indicated that chemical processes alter Keratin's conformation, resulting in detrimental effects on the hair fiber. The cumulative impact of these processes chemically and physically modifies the hair's structure. The findings revealed that the most effective treatment was the High Cost (HC) product, which contains coconut oil and glycerol. This treatment was particularly effective for hair that had undergone significant degradation, particularly from bleaching followed by color treatment. Remarkably, this high-cost (HC) product produced a higher level of morphological restoration than expected, even aiding in the recovery of cuticular cells.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147729424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollen Morphology of Crucianella L. (Rubiaceae) Based on Light and Scanning Electron Microscope Data and Implications for Taxonomy.","authors":"Sevcan Celenk, Zelal Kücük, Gülşah Bağcıvan, Ruziye Daşkın","doi":"10.1002/jemt.70142","DOIUrl":"https://doi.org/10.1002/jemt.70142","url":null,"abstract":"<p><p>Crucianella L. (Rubiaceae) is represented by 31 species worldwide and 14 taxa in Türkiye. Although taxonomic delimitation has traditionally been based on morphological characteristics, the importance of pollen morphology in species differentiation is not yet fully understood. This study was conducted to examine the pollen morphology of Crucianella species in detail using LM and SEM techniques and to assess the taxonomic significance of these details. The pollen grains are stephanocolpate, isopolar, radially symmetrical, and have a circular amb shape. The pollen surface is covered with pollenkitt, and the pollen grains exhibit microreticulate ornamentation. Differences among investigated species were observed in terms of pollen size, exine sculpturing, and aperture characteristics. The number of colpi varies between 6 and 13. Differences in the number of apertures were found among subspecies. Ornamentation was identified as microreticulate. Microechini, perforations and microverrucae were observed on the muri. Pollen size falls within the small class, with the polar axis ranging from 11.41 to 24.85 μm and the equatorial axis ranging from 9.96 to 24.87 μm. The smallest average pollen size was observed in C. kurdistanica (P = 14.90 ± 2.30 μm; E = 13.46 ± 2.11 μm), while the largest was recorded in C. bithynica (p = 21.92 ± 1.86 μm; E = 20.99 ± 2.54 μm). Exine thickness ranged from 0.54 to 1.15 μm, and intine thickness ranged from 0.33 to 0.59 μm. This study demonstrated that palynological characters within the genus Crucianella especially pollen size, aperture details, and the thickness of exine and intine, can provide valuable contributions to the systematic differentiation of closely related species and subspecies.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147717350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Modifications in the Adhesive Organ of a Hillstream Catfish Hara hara : Scanning Electron Microscopy and Histochemistry.","authors":"Jitendra Kumar Sharma, Usha Kumari, Swati Mittal, Ajay Kumar Mittal","doi":"10.1002/jemt.70148","DOIUrl":"https://doi.org/10.1002/jemt.70148","url":null,"abstract":"<p><p>In the present study, the adhesive organ (AO) of Hara hara, a hillstream catfish, has been investigated using scanning electron microscopy, histology, and glycoprotein, protein, and lectin histochemistry. The AO is a disc-like structure present on the ventral side of the body. It is distinguished into a major flat central region and a narrow flap-like region at the periphery of the central region. The free surface of the AO is characteristically differentiated into plaques separated by furrows. Surface epithelial cells in the plaques are modified into unicellular spine-like unculi. The furrow epithelium contains three types of unicellular gland cells (the mucous goblet cells, the club cells, and the sacciform cells), along with sensory structures such as the taste buds (TBs). Gland cells are involved in the elaboration of various glycoproteins (oxidizable vicinal diols, carboxyl groups, and O-sulphate esters); protein moieties (basic proteins, cysteine-bound sulphydryl groups, keratin, tyrosine, protein bound-NH₂ groups, and elastin); and glycans (galactose, N-Acetylgalactosamine, and fucose). Presence of these glycoprotein and protein moieties elaborated by the cellular component of the AO has been associated to play an important role in maintaining structural integrity of the epithelium, keratinization, protection against pathogens, and adhesion of the fish to the substratum. The TBs located at the summit of the tubercles in the furrow epithelium may serve as an adaptation to enhance sensitivity to food selection and environmental perception. Overall, this study provides a comprehensive understanding of the structural and functional adaptations that enable H. hara to maintain effective attachment and thrive in turbulent hillstream environments.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Camera-Like Dual-Defocus Curvature Wavefront Sensor With GPU Acceleration for Real-Time Quantitative Phase Imaging","authors":"Wei Wang,&nbsp;Zihao Zhang,&nbsp;Yaxi Li,&nbsp;Huachuan Huang,&nbsp;Aihui Sun,&nbsp;Shouyu Wang","doi":"10.1002/jemt.70106","DOIUrl":"10.1002/jemt.70106","url":null,"abstract":"<div>\u0000 \u0000 <p>Due to the high-contrast imaging capability for label-free cells, diverse quantitative phase imaging (QPI) systems have been developed. However, most existing solutions exhibit limitations, including incompatibility with commercial microscopes, bulky/complex architectures, and restricted frame rates caused by computationally intensive phase reconstruction processes, thereby hindering their applicability in dynamic QPI scenarios. To overcome these challenges, we developed a camera-like curvature wavefront sensor (CWS) that integrates simultaneous dual-view transport-of-intensity phase imaging with parallel computing. The cost-effective system comprises a prism and dual-CMOS sensor within a compact size of 55.7 × 58.0 × 49.4 mm³ compatible with commercial microscopes. Operating at 20 frames per second (fps) with 1024 × 1024-pixel resolution, it enables real-time image acquisition, phase retrieval, data storage, and result visualization. Experimental validation confirmed its robust performance in field-of-view (FoV) correction, phase recovery accuracy, and computational efficiency. Practical utility was demonstrated through QPI-based flow cytometry and live-cell dynamic imaging applications. This plug-and-play, imaging system compatible, and cost-effective CWS platform offers a versatile solution for practical QPI requirements.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 5","pages":"705-717"},"PeriodicalIF":2.1,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145756888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerating Prostate Cancer Detection Through Histopathological Image Analysis Using Artificial Intelligence","authors":"Anandh Sam Chandra Bose,&nbsp;Chandran Srinivasan,&nbsp;Chandrasekaran Saravanakumar","doi":"10.1002/jemt.70104","DOIUrl":"10.1002/jemt.70104","url":null,"abstract":"<div>\u0000 \u0000 <p>Prostate cancer is a prevalent and serious health concern, ranking among the most frequently diagnosed cancers and a leading cause of cancer-related deaths in men worldwide. Early detection and accurate diagnosis are crucial for improving patient outcomes by limiting disease progression. Histopathological image analysis remains the gold standard for prostate cancer detection; however, manual interpretation is time-consuming and requires specialized expertise. To address these challenges, this study proposes a hybrid deep learning framework that combines an ensemble of transfer-learned CNNs (VGG-16, DenseNet-121, and AlexNet) with a fine-tuned Vision Transformer (ViT). The CNN ensemble extracts rich local features, while the ViT captures global contextual dependencies through a self-attention mechanism and a multilayer perceptron (MLP). Additionally, a cross-attention fusion (CAF) module integrates local and global features, and knowledge distillation (KD) enables a lightweight student network suitable for efficient clinical deployment. The study utilizes the publicly available PANDA dataset for training and testing. Preprocessing steps, including patch generation, gamma correction, and stain deconvolution, enhance image quality and feature representation. A comprehensive evaluation was conducted using standard performance metrics such as accuracy, true positive rate (TPR), true negative rate (TNR), precision, F1-score, false negative rate (FNR), and false positive rate (FPR). An ablation study confirmed the contribution of each module, highlighting the critical role of ensemble CNNs, CAF, and ViT in improving performance. Experimental results demonstrate that the proposed model outperforms conventional transfer learning models and existing state-of-the-art techniques, achieving 97.91% accuracy, along with significant improvements in TPR, TNR, and reduced FNR/FPR. The computational complexity, evaluated in terms of parameters, FLOPs, GPU memory, and inference time, indicates that the proposed model is more demanding than traditional CNNs. Nevertheless, the architecture strikes a practical balance between predictive accuracy and efficiency, making it suitable for real-world clinical applications. These findings underscore the potential of AI-powered hybrid models in expediting prostate cancer diagnosis and enabling timely intervention for improved patient outcomes.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 5","pages":"687-704"},"PeriodicalIF":2.1,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145724718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic Synthesis of Cerium Oxide Nanoparticles: Characterization, Biological Activities and Their Corrosion Inhibition Properties","authors":"N. Muthulakshmi,&nbsp;M. Senthil,&nbsp;B. Archana,&nbsp;A. Mani,&nbsp;R. Subramanian","doi":"10.1002/jemt.70109","DOIUrl":"10.1002/jemt.70109","url":null,"abstract":"<div>\u0000 \u0000 <p>Cerium oxide nanoparticles (CeO₂ NPs) were synthesized via a green and eco-friendly method using <i>Parkia biglandulosa</i> leaf extract, which acted as both a reducing and stabilizing agent. The synthesized CeO₂ NPs exhibited a cubic fluorite crystal structure and predominantly spherical morphology, with particle sizes ranging from 10 to 12 nm. Antibacterial studies demonstrated notable activity against <i>Lactobacillus acidophilus</i>, <i>Staphylococcus albus</i>, and <i>Streptococcus mutans</i>, with the highest inhibition zone (32 mm) observed against <i>S. mutans</i> (Ce-3). The anticancer activity assessed against MCF-7 breast cancer cells demonstrated an IC₅₀ value of 43.13 μg/mL (Ce-3). Antioxidant assays, including DPPH, ABTS, and hydroxyl radical scavenging, exhibited IC₅₀ values of 202.47, 219.77, and 193.05 μg/mL, respectively, indicating strong free radical scavenging potential. Additionally, corrosion resistance studies of CeO₂ NP-coated mild steel in 3.5% NaCl solution revealed a maximum inhibition efficiency of 95.44% (Ce-3), as confirmed by electrochemical impedance spectroscopy. Overall, these findings demonstrated the multifunctional efficacy of <i>P. biglandulosa</i> derived CeO₂ NPs for biomedical and industrial applications.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 5","pages":"727-740"},"PeriodicalIF":2.1,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145810684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}