Microscopy Research and Technique最新文献

{"title":"Interaction of NK-92 Immune Cells and HepG2 Cancer Cells Studied by Atomic Force Microscopy.","authors":"Xinxin Shao, Yue Wang, Liguo Tian, Ying Wang, Yanling Tian, Jiajia Wang, Zuobin Wang","doi":"10.1002/jemt.70186","DOIUrl":"https://doi.org/10.1002/jemt.70186","url":null,"abstract":"<p><p>This paper studied the interaction between HepG2 cancer cells and NK-92 immune cells, employing an atomic force microscope (AFM) as the primary tool. The research mainly focused on the use of AFM to explore the effect of immune cells on cancer cells and revealed the relationship between the killing effect of immune cells on cancer cells and the mechanical properties of cancer cells. A novel method for quantifying in situ adhesion between the NK-92 cells and HepG2 cells was proposed, which provided insight into the relationship between the mechanical properties and the time of co-culture. Furthermore, the morphological and mechanical changes of HepG2 cells were detected using AFM. As co-culture time increased, the findings revealed a trend toward incomplete cell morphology. Reductions were observed in the heights, adhesion, and elastic moduli of HepG2 cells, along with an increase in cell surface roughness. The surface of membrane pores was visualized, and based on the obtained cell height data, the measurements of the size and depth of cell membrane surface pores were conducted. In conclusion, this research unraveled the complex interplay between the killing effect of immune cells on cancer cells and the mechanical properties of cancer cells. It offered a valuable perspective for understanding the interaction between immune cells and cancer cells, potentially paving the way for future therapeutic interventions.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlative STEM-EELS and First-Principles Probing of Nanoscale Elastic Heterogeneity Around T₁ and Al₃Zr Phases in AA2195 Al-Li-Cu Alloy.","authors":"Mohamed E Daoud, Naga Venkateswara Rao Nulakani, Muna S Khushaim, Mohamed Ben Hassine, Sharmarke Mohamed, Mohamad Akbar Ali, Dalaver H Anjum","doi":"10.1002/jemt.70185","DOIUrl":"https://doi.org/10.1002/jemt.70185","url":null,"abstract":"<p><p>The local elastic response of the Al matrix surrounding T₁ precipitates and Al₃Zr dispersoids in a T8-treated AA2195 Al-Li-Cu alloy was investigated using a correlative approach combining advanced scanning transmission electron microscopy, low-loss electron energy-loss spectroscopy, image simulation, and first-principles calculations. Aberration-corrected HAADF-STEM and STEM-EDS confirmed the presence of plate-like T₁ precipitates and spheroidal Al₃Zr dispersoids, with Cu enrichment in the T₁ phase and Zr enrichment in the dispersoids. Low-loss STEM-EELS spectrum imaging was used to probe the local valence-electron response by analyzing the first bulk plasmon peak of the Al-rich matrix. Quantitative fitting of the plasmon peak revealed spatial variations in peak amplitude, full width at half maximum, and energy position near the precipitate-matrix interfaces, indicating local changes in electron density, bonding environment, and interfacial strain. Using an empirical plasmon energy-elastic modulus relationship, local effective Young's modulus values of approximately 70.4 GPa for the Al matrix, 69.3 GPa for the T₁-adjacent Al-rich matrix/interface region, and 72.1 GPa for the Al₃Zr-adjacent Al-rich matrix/interface region were obtained. These values reveal nanoscale elastic heterogeneity associated with the distinct precipitate-matrix interfacial environments. QSTEM-based multislice HAADF-STEM simulations reproduced the experimental T₁ contrast and validated the Cu-rich layered atomic arrangement of the precipitate. Density functional theory calculations were further performed on idealized Al/T₁ and Al/Al₃Zr interface supercells. Strain-energy analysis yielded effective directional Young's moduli of approximately 78 GPa and 73 GPa, respectively, for the selected interface configurations. The combined experimental and computational results show that the local elastic response of AA2195 is governed by precipitate chemistry, interfacial coherency, lattice mismatch, and electronic-structure modification. This work establishes low-loss STEM-EELS plasmon mapping, supported by atomistic simulations and DFT calculations, as a powerful framework for linking nanoscale structure, chemistry, and elasticity in precipitation-strengthened Al-Li-Cu alloys.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the Structural Characteristics and the Fracture Resistance Mechanism of the Primary Flight Feather Shaft in Vulture.","authors":"Liyan Wu, Huitong Ma, Yuliang Huang, Fangming Cui, Cuihong Liu, Yuqiu Song, Huan Wang, Mingjin Xin","doi":"10.1002/jemt.70184","DOIUrl":"https://doi.org/10.1002/jemt.70184","url":null,"abstract":"<p><p>The primary flight feather shaft (PFF) plays a crucial role in adjusting avian's flight posture and flight speed while bearing intense aerodynamic force, and it demonstrates exceptional mechanical properties and fracture resistance. The influence of the structure of a PFF on its fracture resistance mechanism (including crack initiation resistance and crack propagation resistance) remains unclear. This study focused on the research subject of the PFF of Aegypius monachus (cinereous vulture) and the impact of the macro/microstructural characteristics and components on the fracture resistance mechanism. The results showed that the variation in cross-sectional shape (from circular to square) enhanced the bending stiffness of the PFF. The PFF exhibited a maximum compressive stress of 28.85 ± 0.91 MPa and a maximum tensile strength of 51.59 ± 4.43 MPa. Layered fibers and the bridging structure between fibers inhibited crack initiation and propagation. The medullary foam was composed of closed-cell porous units, which reduced the overall weight of the PFF and absorbed external mechanical energy. The cuticle layer contained 5.97 wt% sulfur (S) and abundant cysteine disulfide bonds, which endowed the PFF surface with high rigidity and inhibited crack generation. This study reveals the influence of structural characteristics of vulture PFF on its fracture resistance mechanism and provides inspiration for the design of bioinspired lightweight composite structures.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149069957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep Learning Assisted-Axial Resolution Improvement for Probe-Based Confocal Laser Endomicroscopy.","authors":"Lin Wang, Xinyu Wang, Yun Zheng, Zhaoyang Cheng, Huan Kang, Xueli Chen","doi":"10.1002/jemt.70182","DOIUrl":"https://doi.org/10.1002/jemt.70182","url":null,"abstract":"<p><p>Probe-based confocal laser endomicroscopy (pCLE), as a powerful medical device for detecting digestive tract diseases, can directly conduct real-time and high-resolution histological diagnosis of gastric mucosal epithelium and subcutaneous tissues. It can achieve optical biopsy and has great potential for early diagnosis of gastric cancer. Deep tissue detection and high axial resolution in the depth direction are of great significance for the diagnosis of clinical gastrointestinal diseases. However, improvements in hardware systems will greatly increase the complexity of the system structure, thereby limiting its wide application. Based on the residual channel attention network (RCAN), we developed a deep learning method for axial resolution enhancement. This method, abbreviated as ARE-RCAN, can directly reconstruct images captured by pCLE into ones with higher axial resolution without any additional hardware design. Firstly, high-quality training and testing datasets are generated through numerical simulation, and based on this, the ARE-RCAN is trained and performance tested. The reconstructed results of the testing datasets show that the axial resolution of the reconstructed images of simulated fluorescent beads has doubled, and the lateral resolution has not changed. Finally, a home-built pCLE system was used to collect fluorescent bead and paper fibers data, and the effectiveness of the ARE-RCAN was verified through experiments. The results indicate that the ARE-RCAN method significantly improves the axial resolution of the actual system.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148990734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A High-Resolution Transmission Electron Microscopy Image Processing Workflow for Extracting Aromatic Lattice Fringes in High-Rank Coal.","authors":"Lin Kejin, Li Huantong, Li Yuyang","doi":"10.1002/jemt.70181","DOIUrl":"https://doi.org/10.1002/jemt.70181","url":null,"abstract":"<p><p>Quantitative extraction of aromatic lattice fringes from high-resolution transmission electron microscopy (HRTEM) images is essential for the nanoscale characterization of high-rank coal. However, variations in image processing strategies may introduce structural biases into the extracted lattice fringe networks, while the effects of individual processing steps remain insufficiently evaluated. In this study, an image processing workflow was developed for lattice fringe extraction from high-resolution transmission electron microscopy images of high-rank coal. The workflow integrates frequency-domain enhancement, contrast optimization, threshold segmentation, skeletonization, and skeleton refinement. Comparative experiments were performed to evaluate the effects of enhancement strategies, segmentation methods, and skeleton cleaning procedures on the extracted lattice fringe networks. Three topological parameters, including endpoint density, junction density, and average branch length, were introduced to assess skeleton integrity, abnormal connections, and structural continuity. The results demonstrate that selective frequency-domain enhancement is critical for maintaining stable skeleton topology, whereas spatial-domain smoothing increases non-physical connections and fragmentation. Although global Otsu thresholding produces comparable geometric measurements, differences remain in topological characteristics, indicating that geometric parameters alone cannot fully describe lattice fringe networks. Length-based filtering effectively removes artifacts with limited influence on network connectivity, while junction removal disrupts the structural framework of the skeleton. The proposed workflow provides a robust approach for quantitative analysis of lattice fringe networks and offers a methodological basis for reliable microscopic characterization of carbon materials.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transmission Electron Microscopy-Based Analysis of Silver Nanoparticle-Induced Ultrastructural Damage and Bioaccumulation in Heterakis dispar (Nematoda: Heterakidae) Under In vitro Conditions.","authors":"Fuad Rzayev, Eldar Gasimov, Ali Nasirov, Mehri Seyidbeyli, Aladdin Eyvazov, Gunay Rzayeva, Aysun Keskin, Adem Keskin, Yegana Mahmudova","doi":"10.1002/jemt.70183","DOIUrl":"https://doi.org/10.1002/jemt.70183","url":null,"abstract":"<p><p>Helminths cause a wide range of diseases in different components of ecosystems and may induce serious pathological conditions in the host organisms. In recent years, nanoparticles have been increasingly used in ecology, veterinary medicine, and biology. In this study, the effect of silver (Ag) nanoparticles on the waterfowl parasite Heterakis dispar (Schrank, 1790), isolated from domestic geese (Anser anser domesticus L., 1758), was investigated under in vitro conditions, with particular emphasis on nanoparticle accumulation patterns and associated ultrastructural changes, studied at the ultrastructural level. In the experiments, H. dispar nematodes collected from the hosts were exposed in vitro to Ag nanoparticles at concentrations of 10, 50, and 100 μg/mL. Control and experimental groups of nematodes were examined using light and transmission electron microscopes. The sizes of Ag nanoparticles in a free state were 9.03-23.82 nm, and particles detected within the helminth organism were approximately 11-17 nm. It was visually observed that the particles were present in multiple layers of the body wall, in the digestive and reproductive organs of the parasitic worm. Silver nanoparticles enter the helminth through the body wall (from cuticle to the hypodermis, muscular layer, and organs located in the pseudocoelomic or body cavity) and the digestive organs (through the intestinal microvilli). At the ultrastructural level, notable alterations were observed in treated specimens. The results prove that Ag nanoparticles have anthelmintic activity.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Subcellular Localization of Iron in Rhizophora mangle Leaves Revealed by Integrated Perls Reaction, TEM, STEM-HAADF, and EDS Analyses.","authors":"Silvia Rodrigues Machado, Hiulana Pereira Arrivabene, Camilla Rozindo Dias Milanez, Margarida Juri Saeki, Yve Canaveze","doi":"10.1002/jemt.70180","DOIUrl":"https://doi.org/10.1002/jemt.70180","url":null,"abstract":"<p><p>Mangrove ecosystems are frequently exposed to high concentrations of iron (Fe) in sediments, resulting in Fe accumulation in plant tissues. Although Fe is an essential micronutrient involved in several metabolic processes, its excess requires efficient mechanisms of compartmentalization and storage to maintain cellular homeostasis. Histochemical detection using the Perls reaction has usually been applied to identify ferric iron (Fe³⁺) in biological tissues; however, the combination of this technique with ultrastructural and elemental analyses remains relatively unexplored in plant cells. In this study, we investigated Fe localization in leaf tissues of Rhizophora mangle L. (Rhizophoraceae), a dominant mangrove species, by combining complementary approaches, including Perls cytochemical reaction, transmission electron microscopy (TEM), scanning transmission electron microscopy coupled with high-angle annular dark-field imaging (STEM-HAADF), and energy-dispersive X-ray spectroscopy (EDS). Perls-positive electron-dense deposits were visualized at the ultrastructural level, and their elemental composition was further characterized by EDS analyses. Fe-containing deposits were detected in the epidermis, mesophyll parenchyma, mucilage cells, and vascular tissues, as well as in multiple cellular compartments, including plastids, mitochondria, vacuoles, cell walls, intercellular spaces, and plasmodesmata, whereas sclerenchyma cells showed no detectable Fe-containing deposits. The combination of Perls reaction with TEM, STEM-HAADF, and EDS provides a complementary approach for high-resolution visualization and elemental characterization of Fe-containing deposits at the subcellular level. This integrated methodology may facilitate the investigation of Fe distribution and compartmentalization in plant tissues under contrasting conditions of Fe availability.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148900880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Method for Evaluating the Optical Properties of Liquid-Liquid Phase Separations Using Laser Scanning Microscopic Spectroscopy.","authors":"Johbu Itoh, Natsuko Fujii, Saori Kohara, Masatoshi Ito, Naoto Suzuki, Eiichiro Nagata","doi":"10.1002/jemt.70172","DOIUrl":"https://doi.org/10.1002/jemt.70172","url":null,"abstract":"<p><p>Liquid-liquid phase separation (LLPS) plays a central role in intracellular compartmentalization and gene regulation. Although optogenetic optoDroplet systems are widely used to study LLPS in living cells, the mechanisms underlying the transition from dynamic liquid-like condensates to operationally defined gel-like assemblies remain poorly understood because intermediate assembly states are difficult to monitor in situ. Here, we developed a confocal laser microspectroscopy-based approach to characterize the assembly dynamics of the fused sarcomas (FUS)n-mCherry-CRY2 optoDroplet system in living NIH3T3 cells. Quantitative fluorescence analysis enabled the identification and discrimination of three distinct assembly states: monomeric, dimer/oligomer intermediate, and gel-like. Passive micro-rheology, fluorescence recovery after photobleaching (FRAP), and automated morphological analyses further demonstrated progressive reductions in molecular mobility and increasing structural rigidity during condensate maturation. Three-dimensional spatial analysis revealed that mature condensates exhibit a distinct core-shell organization consisting of an operationally defined gel-like core, a dimer/oligomer-rich intermediate layer, and an outer monomer-rich boundary layer with liquid-like properties. Upon cessation of blue-light stimulation, the outer layer rapidly dissolved, whereas the central gel-like core remained intact, indicating the acquisition of physical irreversibility. These findings establish fluorescence microspectroscopy as a quantitative approach for resolving molecular assembly states during condensate maturation and demonstrate that gelation proceeds from the condensate center toward the periphery. This framework provides new insights into pathological liquid-to-gel phase transitions associated with neurodegenerative diseases.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148897537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on Vessel Morphological Characteristics and Environmental Adaptability of 10 Gentianaceae Species From the Qinghai-Xizang Plateau.","authors":"Ling Zheng, Yaqi Han, Xiaoyu Zhou, Pengcheng Fu","doi":"10.1002/jemt.70179","DOIUrl":"https://doi.org/10.1002/jemt.70179","url":null,"abstract":"<p><p>This study employed scanning electron microscopy (SEM) to conduct a morphological observation of vessel elements from 10 Gentianaceae species collected on the Qinghai-Xizang Plateau. We systematically compared vessel type, end-wall and lateral-wall characteristics, and vessel diameter to explore their adaptive relationship to the extreme plateau environment and the evolutionary trends of vessels. The results indicated that the vessel end walls of all 10 species featured simple perforation plates, while annular, spiral, and scalariform thickenings were observed on the lateral walls of all species, with scalariform vessels constituting the highest proportion in most. The vessel types were primarily annular, spiral, and scalariform; pitted vessels were found only in Gentiana farreri and G. waltonii. The vessel diameters were generally small (mostly < 20 μm). The vessel morphology exhibited significant adaptive characteristics to plateau adversities such as low temperature, drought, and intense radiation. Based on the analysis of vessel type, end wall features, and lateral wall characteristics, the vessels of G. farreri and G. waltonii exhibited the highest proportions of morphologically specialized types (pitted and scalariform vessels) according to the classical Bailey-Frost sequence, whereas those of G. aristata and G. hexaphylla were dominated by the more generalized annular and spiral types. This research provides new anatomical evidence for understanding the structural adaptation mechanisms of Gentianaceae plants in high-altitude environments and offers a theoretical basis for the conservation and utilization of these plant resources.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148888157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Identification of Multiple Types of Adulterated Ophiocordyceps sinensis by SEM-EDS Based on Micromorphology and Elemental Analysis.","authors":"Yi-Wei Liu, Li-Xing Nie, Yu Wu, Zheng-Ming Qian, Kai Guo, Zhe Li, Yi Zhang, Jian-Dong Yu, Xian-Long Cheng, Yong-Qiang Lin","doi":"10.1002/jemt.70171","DOIUrl":"https://doi.org/10.1002/jemt.70171","url":null,"abstract":"<p><p>As a rare and precious traditional Chinese medicine (TCM), Ophiocordyceps sinensis (O. sinensis) is plagued by various adulterants in the market due to factors such as resource scarcity, which seriously affects medication safety and clinical efficacy. Existing identification methods generally have problems including strong subjectivity, high sample destructiveness, long detection cycle, and complex operation, and it is difficult to identify different types of adulterants using the same method. Innovatively, this study adopted scanning electron microscopy-energy dispersive x-ray spectroscopy (SEM-EDS) technology to establish a rapid, accurate, and widely applicable method for identifying adulterated O. sinensis. Authentic O. sinensis samples from major producing areas such as Xizang, Qinghai, Sichuan, and Gansu, as well as different types of adulterated samples, were collected. Using SEM-EDS technology, combined with micromorphology and elemental composition, the differences between authentic and adulterated samples were systematically revealed through indicators including morphological differences, mass fraction of characteristic elements, and surface area coverage (SAC) of characteristic elements in surface scanning. This study confirmed that SEM-EDS technology could simultaneously obtain the micromorphological and elemental distribution information of samples, realizing rapid and accurate identification of multiple types of adulterated O. sinensis. The proposed method provided new technical support for the quality control and market supervision of O. sinensis, which could be extended to the adulteration inspection of other precious and delicate TCM.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}