Microscopy Research and Technique最新文献

{"title":"Catalytic Degradation by Metal-Organic Hybrid Nanocomposites Based on Metal/Metal Oxide Nanostructured Materials Prepared by Pulsed Laser Ablation.","authors":"Nada S Al-Kadhi, Ayman M Mostafa, Ghadah M Al-Senani, Ameenah N Al-Ahmadi, Fowzia S Alamro, Hoda A Ahmed, Rawan Al-Faze, Wafaa B Elsharkawy, Eman A Mwafy","doi":"10.1002/jemt.24858","DOIUrl":"https://doi.org/10.1002/jemt.24858","url":null,"abstract":"<p><p>The heterojunction based on plasmonic metals of Ag and Cu in the oxide form was synthesized and decorated carbon nanotubes to form Ag-Cu₂O/MWCNTs nanocomposites via a pulsed laser ablation process for water treatment. This study presents the synthesis and characterization of Ag/MWCNTs nanocomposites with different amounts of Cu₂O NPs using a novel two-step pulsed laser ablation technique for enhanced photocatalytic degradation of Congo Red dye under UV-visible-light irradiation. Structural and optical characterization techniques confirmed the successful formation of highly pure and crystalline nanocomposites, and the coupling of Ag NPs with Cu₂O NPs significantly enhanced visible-light absorption, making the nanocomposites highly effective for photocatalytic applications, which were systematically evaluated under varying pH, catalyst dosage, dye concentration, and irradiation time conditions. The results demonstrated that Ag-Cu₂O/MWCNTs with 60 min ablation (30min for Cu and 30 min for Ag), Ag-Cu₂O/MWCNTs(2), achieved the highest degradation efficiency of 99.43% for Congo Red (CR) dye at pH 8.0 and an optimal catalyst dosage. Kinetic studies and the degradation mechanism revealed that the photodegradation process followed the Langmuir-Hinshelwood pseudo-first-order model, with Ag-Cu₂O/MWCNTs(2) exhibiting the fastest degradation rate, outperforming other samples by degrading CR dye. This work underscores the potential of Ag-Cu₂O/MWCNTs nanocomposites as highly efficient photocatalysts for environmental remediation, particularly in the degradation of organic pollutants in wastewater. The findings provide valuable insights into optimizing photocatalytic processes for practical applications, offering a promising solution for sustainable water treatment and pollution control.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Effectiveness of Different Caries Removal Methods in Primary Teeth With Micro-Computed Tomography and Scanning Electron Microscopy-Energy Dispersive Spectroscopy of X-Ray.","authors":"Suna Nur Gokce, Arda Buyuksungur, Levent Ozer","doi":"10.1002/jemt.24868","DOIUrl":"https://doi.org/10.1002/jemt.24868","url":null,"abstract":"<p><p>This study evaluated the effectiveness of conventional rotary instruments (CRI), atraumatic restorative treatment (ART), and the BRIX-3000 chemomechanical caries removal gel by measuring bone mineral density (BMD) and mineral composition in vitro. The carious dentin of 50 extracted human primary molar teeth was removed by using three different methods and restored with high-viscosity glass ionomer cement FUJİ-IX. The BMD values measured from all sections of the cavity floor in the BRIX-3000, ART, and CRI groups are 1.72, 1.79, and 1.66 g/cm³, respectively. SEM-EDX measurements were performed on five randomly selected teeth from the groups. In the BRIX-3000 group, the mean wt Ca% was 65.6, wt P% was 25.34, and the Ca/P ratio was 2.57. In the ART group, 65.75, 23.79, and 2.58 were found, respectively. In the CRI group, 65.73, 26.03, and 2.53 were found, respectively. While there was no difference between the wt Ca% values in all groups in the comparison of the cavity floor and adjacent sound dentin of the samples, the wt P% values were statistically significantly higher on the sound dentin surface.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin Healing Potential of Bioactive Components From Lycoperdon lividum Mushroom Versus β-Sitosterol in Rat Model of Burn Wounds.","authors":"Mohammed Radhi Thammer, Hayder B Sahib, Hayder Ridha-Salman","doi":"10.1002/jemt.24864","DOIUrl":"https://doi.org/10.1002/jemt.24864","url":null,"abstract":"<p><p>Burn injury elicits an inflammatory response, metabolic abnormalities, and distributional shock leading to diverse organ dysfunctions with remarkable morbidity and mortality. Sluggish wound healing, infections, and hypertrophic scars still struggle in burn treatment. Lycoperdon lividum, a puffball mushroom enriched in polyphenols, has outstanding antioxidative and anti-inflammatory properties. This study aimed to assess the benefits of L. lividum on second-degree burn lesions in a rat model at 100°C. Seventy-five male rats were haphazardly separated into five groups of 15 rats each: Control (without any skin burn induction/treatment), skin burn (skin burn induction/non-treated), β-sitosterol (treated with topical β-sitosterol 0.25%), Vehicle (treated with Vaseline), and L. lividum (treated with topical L. lividum watery extract 10%). Rats were euthanized on Days 5, 10, and 20 of the experiment. Lycoperdon lividum extract was analyzed using HPLC techniques. Skin samples were collected for microscopical and histopathological examinations. Biomarkers involving IL-6, IL-10, TGF-β1, TNF-α, and VEGF were analyzed in skin homogenates. The chromatogram revealed distinct peaks for bioactive compounds of L. lividum, including kaempferol, rutin, ferulic acid, caffeic acid, chlorogenic acid, quercetin, and gallic acid. Lycoperdon lividum greatly alleviated the burn lesions via improving wound contraction, correcting inflammation, and granulation aberrations while boosting fibrosis. The outcomes were validated by histopathological analyses. Further, L. lividum markedly improved the healing of burn wounds by regulating inflammatory cytokines (IL-6 and TNF-α), anti-inflammatory cytokines (IL-10 and TGF-β1), and angiogenic cytokines (VEGF) in skin tissues. Lycoperdon lividum has potential in healing burn wounds thanks to its outstanding antioxidant and anti-inflammatory actions.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of New Copolymer and Genotoxic Effects on Triticum aestivum L. (Wheat) Root Tip Cells.","authors":"Neslihan Taşar, Gülben Torgut","doi":"10.1002/jemt.24873","DOIUrl":"https://doi.org/10.1002/jemt.24873","url":null,"abstract":"<p><p>In the present study, a copolymer of poly(N,N-dimethylacrylamide) and sodium acrylate was synthesized with the Solution Polymerization Technique. The purpose was to uncover the cytotoxic and genotoxic effects on root tip cells of the Triticum aestivum L. plant germinated with this polymer at different concentrations (25, 50, 100, and 200 ppm). The deposition of poly(DMAA-co-NaAc) copolymer in the study sample T. aestivum was demonstrated with the FTIR, UV-VIS spectroscopy analysis, X-Ray Diffraction (XRD) Spectroscopy, Scanning Electron Microscopy (SEM), SEM Element Map, and Energy Dispersive Spectroscopy (EDS) Spectrum. All concentrations of the poly(DMAA-co-NaAc) copolymer caused a decrease in the Mitotic Index (MI). The poly(DMAA-co-NaAc) copolymer caused various mitotic abnormalities such as loss of genetic material, deconstructed prophase, adhesion, chromosome groupings in metaphase, deconstructed metaphase, C-metaphase, chromosomal loss, chromosomal fracture, deconstructed anaphase, lagging chromosome, fragment, polar aberration, bridge, advancing, star anaphase, multipolarity, and deconstructed telophase. Study results show that poly(DMAA-co-NaAc) copolymer will cause abnormalities in mitosis as a result of its uptake by plants. These results also show that the poly(DMAA-co-NaAc) polymer, which affects organisms, must be produced in a controlled setting to reduce its accumulation in nature and ultimately disposed of in a way that will not harm the environment.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Juglone as a Therapeutic Agent in Murine Coccidiosis: Enhancing Intestinal Recovery and Regulating Host Immune Responses.","authors":"Shaimaa M Kasem, Mohamed A Dkhil, Amal M Marey, Rewaida Abdel-Gaber, Felwa A Thagfan, Sherif Elshanat","doi":"10.1002/jemt.24871","DOIUrl":"https://doi.org/10.1002/jemt.24871","url":null,"abstract":"<p><p>Coccidiosis, caused by Eimeria spp., significantly impacts gastrointestinal health and leads to economic losses. This study investigated juglone, a phenolic compound, as a potential treatment for mouse coccidiosis caused by Eimeria papillata. Twenty-five male Swiss albino mice were categorized into five groups: Group 1: non-infected, Group 2: juglone control (JG), Group 3: infected with 1,000 sporulated oocysts, Group 4: infected-treated with juglone (Infected + JG), and Group 5: infected-treated with anticoccidial drug, amprolium (Infected + ACD). Following 5 days of treatment, the mice were euthanized, and numerous analyses were performed such as growth performance, oocyst shedding, oxidative stress, histology, goblet cell response, and inflammation. Results showed that E. papillata infection significantly decreased body weight and feed intake, increased oocyst shedding, and elevated oxidative stress markers while reducing antioxidant enzyme levels. Histopathological analysis indicated severe intestinal damage, with intracellular parasitic stages and reduced goblet cell density and MUC-2 expression. Infection also heightened gene expression of inflammatory cytokines including tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ), interleukin 1β (IL1β), and interleukin 10 (IL10). Juglone treatment effectively mitigated these negative effects, improving body weight and feed consumption, lowering oocyst shedding, and restoring antioxidant enzyme activity. It also repaired intestinal structure, enhanced goblet cell density and MUC-2 expression, as well as diminished inflammatory cytokines. The findings indicated that juglone significantly mitigates the pathological effects induced by E. papillata by decreasing parasite shedding, oxidative stress, inflammation, and modulating immune responses while facilitating intestinal recovery and enhancing overall health in infected mice.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards Understanding the Effect of Loading Direction on Fatigue Crack Behavior in Mg-3Al-Zn Magnesium Alloy.","authors":"Zhanghu Shi, Pan Tang, Hua Rao, Pei Lei, Deng Pan, Junshan Hu, Wei Tian","doi":"10.1002/jemt.24869","DOIUrl":"https://doi.org/10.1002/jemt.24869","url":null,"abstract":"<p><p>As the lightest structural metal material, magnesium alloy is increasingly widely used in aerospace, rail transportation and other fields. However, during its service, the action of alternating loads often induces fatigue fracture damage, which seriously threatens its service safety and stability. This study investigates the fatigue behaviors of Mg-3Al-Zn (AZ31) magnesium alloy along its rolling direction (RD) and normal direction (ND) under strain-controlled tension-tension cyclic loading. The strain-life curve reveals that ND specimens in which the deformation was dominated by twinning-detwinning exhibits higher fatigue life than RD specimens dominated by slip. Morphological analysis of crack patterns indicates many grain boundary fractures and long surface cracks in RD specimens, contrasting with the ND specimens' uniform distribution of short, linear cracks throughout the texture. Scanning Electron Microscopy (SEM) and Electron Backscatter Diffraction (EBSD) analyses suggest that crack initiation in RD specimens is likely at grain boundaries due to deformation incompatibility between the grains with soft and hard directions. Conversely, the cracks of ND specimen predominantly formed at the {10 <math> <semantics> <mrow><mover><mn>1</mn> <mo>¯</mo></mover> </mrow> <annotation>$$ overline{1} $$</annotation></semantics> </math> 2} twinning boundaries, owing to the strain incompatibility between the matrix and twin due to the discrepancy of basal dislocation activations.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructural Architecture and Morphological Examination of Hirudo verbana (Annelida, Hirudinea).","authors":"Sedef Selviler Sizer, Hakan Eren, Cansu Aktaş, Mehmet Pazar, Esra Demirbas, Burcu Onuk, Ertan Emek Onuk, Gokmen Zafer Pekmezci, Murat Kabak","doi":"10.1002/jemt.24859","DOIUrl":"https://doi.org/10.1002/jemt.24859","url":null,"abstract":"<p><p>In this study, the morphological characteristics of the medicinal leech Hirudo verbana (Annelida, Hirudinea) were examined. Mitochondrial CO1 gene sequences of representative specimens were recorded in GenBank, and macroscopic, histological, and SEM analyses of remaining representatives were made. The species had trignathous jaws, each half-moon in shape. There were 75-80 denticles on the left jaw with their tips medially, while the right jaw had 30-34 equilateral triangular denticles, all arranged in a single row. The anterior jaw had 35-40 blunt-tipped denticles similarly arranged in a single row. Salivary papillae of varying sizes were scattered along the edges of the jaws, with salivary pores appearing as irregularly distributed round openings. Five pairs of dorsal eyes appeared round under a stereomicroscope but oval under SEM. Seventeen pairs of round nephridial pores were symmetrically located on every fifth annulus of the ventral side. Grape-cluster-like cells containing granules were observed between the crop and the muscle layer. Bean-shaped testisacs and coiled vas deferens canals were bilaterally arranged near the crop. Thread-like extensions and sensilla were identified across the ventral annuli. Beneath the epidermis, circular, oblique, and longitudinal muscle layers and three types of secretory cells between them were identified. As a result, this study has identified differences in the denticle morphology of H. verbana and has contributed these findings to the literature. Additionally, it was found that there are differences in the shape of the examined structures between stereomicroscope and SEM images in small and complexly structured organisms.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alternatives for Uranyl Acetate Negative Staining With Respect to the Resolution Obtained After Single Particle Analysis of Erythrocruorin.","authors":"Foteini Karapanagioti, Nicolas Cissé, Anastasiia Atamas, Artem Stetsenko, Christiaan Michiel Punter, Erica Zuidersma, Marc C A Stuart","doi":"10.1002/jemt.24865","DOIUrl":"https://doi.org/10.1002/jemt.24865","url":null,"abstract":"<p><p>Although cryo-electron microscopy is widely used for determining high-resolution structures, negative staining of proteins and supramolecular aggregates remain a valuable screening method, especially for low-abundant proteins. The most commonly used contrasting agent is uranyl acetate (UAc). However, due to its toxicity and the strong regulations connected to the use of uranium compounds, there is an ongoing search for alternative stains, with the focus being shifted to lanthanide and tungsten compounds. In this study, the performance of neodymium acetate (NdAc), europium acetate (EuAc), ytterbium acetate (YbAc), phosphotungstic acid, and the commercially available UA-Zero as negative staining agents for single molecule electron microscopy is evaluated. We focus not only on the observed contrast but also on the resolution that can be obtained after negative staining and single-particle analysis. The findings demonstrate that NdAc, EuAc, and UA-Zero can be good alternatives for the overall superior UAc. Additionally, we explore the working pH range of the stains and discuss the usability of the compounds in terms of availability and regulations.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocatalytic Degradation of Synozol Navy Blue Dye by Ni-Cu-Ag Dopped ZnO: An Insight Into Photoluminescence, Scavenging, Mechanism and Kinetic Aspects.","authors":"Zahid Mahmood, Muhammad Alamzeb, Tahseen Ghous, Sidra Rashid, Zulfiqar Ali","doi":"10.1002/jemt.24867","DOIUrl":"https://doi.org/10.1002/jemt.24867","url":null,"abstract":"<p><p>Cost-effective, sustainable, and eco-friendly strategies are crucial for treating organic pollutants in industrial wastewater. ZnO is a promising photocatalyst due to its affordability, environmentally benign nature, and ability to fully mineralize pollutants. However, its limited effectiveness under visible light is a major drawback. Ni doping helps reduce charge carrier recombination, enhancing the photocatalytic activity of ZnO. Ni-Cu co-doping increases saturation magnetization by creating intrinsic defects, though it can lead to nanocluster formation on the catalyst surface. Ag further improves the performance by preventing nanocluster aggregation and prolonging charge carrier lifetimes. In this study, Ni-Cu-Ag@ZnO nanoparticles were synthesized via the sol-gel method and used as a photocatalyst for the degradation of Synozol navy blue dye. Characterization techniques like FTIR, SEM, XRD, EDX, BET, UV, and PL confirmed the nanocomposite structure and morphology. The Ni-Cu-Ag@ZnO nanocomposite achieved 88% photodegradation efficiency under optimal conditions (a contact time of 50 min, an initial dye concentration of 75 ppm, a catalyst dosage of 0.03 g, and an acidic medium with a pH of 2). The rate constants, k₁ (2 × 10^-2 min^-1) suggest that the degradation of Synozol navy blue by Ni-Cu-Ag@ZnO under ordinary light follows pseudo-first-order kinetics. Scavenging tests further confirmed that the enhanced photocatalytic degradation was driven by the generation of OH^• radicals, with the addition of hydrogen peroxide playing a key role in preventing electron-hole recombination. This indicates that the quantity of hydroxyl radicals and their adsorption on the photocatalyst are crucial in determining the extent of degradation. This study highlights the potential of Ni-Cu-Ag@ZnO nanocomposites for industrial wastewater treatment and opens new avenues for the further enhancement of the photocatalytic efficiency of other catalysts under consideration.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Caries Eradication Techniques via Papacarie, and Er: YAG Laser With Different Adhesive Types Bonded to Composite Restoration in Primary Dentin. An SEM, μTBS, and Microleakage Assessment.","authors":"Faisal Ali Bin Abbooud AlQhtani","doi":"10.1002/jemt.24863","DOIUrl":"https://doi.org/10.1002/jemt.24863","url":null,"abstract":"<p><p>Influence of different caries removal techniques using Papacarie and Erbium/yttrium-aluminum-garnet (Er: YAG) laser to different adhesives etch and rinse (ER) and self-etch (SE) restored via tooth-colored material on micro tensile bond strength (μTBS) and marginal leakage scores. Ninety-six extracted primary molars exhibiting moderate caries were allocated into three groups based on the caries removal technique. Group 1: Conventional bur, Group 2: Papacarie gel, and Group 3: Er: YAG laser. After caries removal, specimens from three groups were further allocated into two subgroups based on the type of adhesive used (n = 16). Two-step ER (A) and Two-step SE (B). Composite restoration was built and thermocycling was performed. Microleakage, μTBS, and failure mode were analyzed using a dye penetration test, a Universal testing machine, and a stereomicroscope respectively. Scanning electron microscopy (SEM) evaluation of the composite-CAD interface was performed. One-way ANOVA and the Tukey HSD test were utilized to compare microleakage and μTBS among investigated groups (p < 0.05). The lowest microlekage (13.56 ± 0.02) and highest μTBS (13.26 ± 0.11 MPa) was exhibited by Group 3B (Er: YAG laser-SE). However, the highest microleakage (45.62 ± 0.09) and lowest bond strength (9.34 ± 0.11 MPa) was exhibited by 1A (Conventional bur-ER) treated samples. For caries removal in primary dentition, the Er: YAG laser and Papacarié gel present themselves as appropriate alternatives. These non-invasive methods yield improved mechanical results when utilized with self-etch adhesive system.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}