Microscopy Research and Technique最新文献

{"title":"pH-induced changes in IgE molecules measured by atomic force microscopy.","authors":"Jing Hu, Zuobin Wang, Dayong Jiang, Mingyan Gao, Litong Dong, Mengnan Liu, Zhengxun Song","doi":"10.1002/jemt.24660","DOIUrl":"10.1002/jemt.24660","url":null,"abstract":"<p><p>The environment surrounding proteins is tightly linked to its dynamics, which can significantly influence the conformation of proteins. This study focused on the effect of pH conditions on the ultrastructure of Immunoglobulin E (IgE) molecules. Herein, the morphology, height, and area of IgE molecules incubated at different pH were imaged by atomic force microscopy (AFM), and the law of IgE changes induced by pH value was explored. The experiment results indicated that the morphology, height and area of IgE molecules are pH dependent and highly sensitive. In particular, IgE molecules were more likely to present small-sized ellipsoids under acidic conditions, while IgE molecules tend to aggregate into large-sized flower-like structures under alkaline conditions. In addition, it was found that the height of IgE first decreased and then increased with the increase of pH, while the area of IgE increased with the increase of pH. This work provides valuable information for further study of IgE, and the methodological approach used in this study is expected to developed into AFM to investigate the changes of IgE molecules mediated by other physical and chemical factors. RESEARCH HIGHLIGHTS: The ultrastructure of IgE molecules is pH dependent and highly sensitive. IgE molecules were tend to present small-sized ellipsoids under acidic pH. Alkaline pH drives IgE self-assembly into flower-like aggregates.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141752079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic efficacy of Strobilanthes urticifolia-infused pectin/polyacrylic acid hydrogel for targeted hepatorenal fibrosis mitigation: A multifaceted biomaterial approach.","authors":"Nosheen Masood, Hajra Hameed, Muhammad Tariq, Rashid Ahmed, Mehwish Khalid, Noreen Latief, Anwarul Hasan","doi":"10.1002/jemt.24667","DOIUrl":"10.1002/jemt.24667","url":null,"abstract":"<p><p>Pectin/polyacrylic acid (PPAA) hydrogel is a unique and versatile biomaterial with applications in drug delivery, wound healing, tissue engineering, and agriculture, owing to its tailored properties and multifunctional attributes. This study aims to harness the therapeutic potential of Strobilanthes urticifolia extract within a PPAA hydrogel matrix to attenuate liver and kidney fibrosis through targeted and sustained delivery of biologically active substances. PPAA hydrogel was prepared by free radical polymerization, followed by its porosity and swelling determination. The results depicted the porous nature of PPAA hydrogel and improved swelling properties at pH 7.4, confirming its drug delivery promise. The polyphenolic-enriched S. urticifolia extracts of leaf and flower were loaded onto PPAA hydrogel, and the loading efficiency was 87% (leaf) and 62.5% (flower). Moreover, slow-release studies showed controlled and prolonged release of polyphenols for 7 days. The polyphenolic-enriched hydrogel's microstructure was characterized using SEM, FTIR, and thermogravimetric analysis (TGA). SEM results revealed a highly porous structure of polyphenol enriched PPAA hydrogel, while FTIR analysis confirmed the presence of functional groups such as OH group of carboxylic acid, aliphatic CH₂ stretching due to acrylic acid grafting with pectin, CO stretching due to acid linkage with pectin, CH of aromatic ring, and CH of carboxylate salt in PPAA hydrogel. TGA of PPAA hydrogel showed its stability up to 488°C. Additionally, the S. urticifolia extract loaded PPAA hydrogel displayed significant antibacterial properties and minimum inhibitory concentrations against both Gram-positive and Gram-negative bacteria. In vivo studies carried out on rats demonstrated that polyphenolic enriched PPAA hydrogel significantly attenuates liver and kidney fibrosis. Therefore, it is concluded from the present study that loading of polyphenolic enriched extract from leaves and flower of S. urticifolia enhanced the biomedical applications of PPAA hydrogel. RESEARCH HIGHLIGHTS: The PPAA hydrogel developed in this study exhibits a highly porous structure and improved swelling properties at physiological pH (7.4), making it an excellent candidate for drug delivery systems. S. urticifolia extracts, rich in polyphenols, were successfully incorporated into the PPAA hydrogel with high loading efficiencies of 87% for leaf and 62.5% for flower extracts. Loading of polyphenolic enriched extracts of S. urticifolia onto PPAA enhanced its biological activities such as antibacterial, hepatoprotective, and reno-protective activities as depicted by in vitro and in vivo studies.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141792877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Presence of abnormal otoliths in hallucinogenic fish and their comparison with normal otoliths using light and scanning electron digital imaging.","authors":"Serdar Yedier, İsmail Burak Daban, Yusuf Şen, Derya Bostancı","doi":"10.1002/jemt.24670","DOIUrl":"10.1002/jemt.24670","url":null,"abstract":"<p><p>The otolith organs located in the inner ear of the fish are responsible for vital activities such as balance and hearing. Abnormalities in these organs can adversely affect the vital activities of the fish species. The main purpose of the study is to analyze the abnormalities in the otoliths of Sarpa salpa, known as the hallucinogenic fish. For that, 372 individuals of S. salpa are collected from the North Aegean Sea. As a result of the abnormality analyses in S. salpa otoliths, anomalies were detected such as various prominence structures on the surface of the otolith caused by accumulation and a more transparent appearance due to the different crystal structures in some parts of the otolith. These abnormalities were found in the left and/or right otoliths of male and female individuals in different total lengths. The percentage of individuals with abnormal otoliths of S. salpa is calculated as 52.42%. It was determined that there are statistical differences between the left and right otolith measurements of male and female individuals with abnormal and normal otoliths(p < 0.05). There is no relationship between the percentage of individuals showing abnormality and total length and sex. The current study presents for the first time abnormal otolith information on left and right otoliths in male and female S. salpa. It is thought that abnormalities in hallucinogenic fish otoliths could be related to genetic predisposition as well as stress due to nutritional preference, pollutants, and environmental factors. RESEARCH HIGHLIGHTS: The presence of abnormalities in the otoliths of Sarpa salpa, a hallucinogenic fish, was revealed for the first time. Abnormalities in the otoliths of S. salpa were identified, such as the presence of various prominence structures on the otolith's surface, loss of parts as well as a more transparent appearance in the outlines or surface of the otolith. Normal and abnormal otoliths of female and male hallucinogenic fish from different size groups were examined using light and scanning electron microscopy. Abnormality detected in the otoliths of hallucinogenic fish is not related to the gender and size of the fish.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141907104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Insights Into the Ultrastructural and Immunofluorescence Characteristics of Limb Skin in the Red-Eared Slider Turtle (Trachemys scripta elegans).","authors":"Mohamed A M Alsafy, Samir A A El-Gendy, Samar M Ez Elarab, Ahmed A El-Mansi, Mamdouh B Eldesoqui, Ahmed M Rashwan","doi":"10.1002/jemt.24729","DOIUrl":"https://doi.org/10.1002/jemt.24729","url":null,"abstract":"<p><p>The red-eared slider turtle, a species facing environmental challenges and habitat loss, exhibits a complex skin architecture that is crucial for its adaptation and survival. Our study aims to provide a comprehensive characterization of the turtle's skin structure and to elucidate the distribution and localization of its various cellular components, with a focus on understanding the skin's role in adaptation and ecological interactions. To achieve these goals, we employed light microscopy, transmission electron microscopy (TEM), and comprehensive immunofluorescence using 10 specific antibodies. The forelimb skin displays large- and moderate-sized scales with variations in color, including dark, yellow, and gray hues, likely contributing to camouflage and protection. The skin consists of corneous material, the epidermis, the dermis, and the hypodermis. The stratum basalis, stratum spinosum, and peri-corneous layer constitute the three distinct layers of the epidermis. There are four distinct types of chromatophores, including melanocytes located in the epidermis, while melanophores, xanthophores, and iridophores are found within the dermal layer. The skin also exhibits well-developed peripheral nerves, blood vessels, and subcutaneous muscles. Immunofluorescence staining further elucidates the distribution and localization of various skin cells. E-cadherin and CK14 are strongly expressed in the epidermal layers, excluding the corneous material. E-cadherin surrounds keratinocyte cells in the epidermis, facilitating cell-cell adhesion, while CK14 is present inside the keratinocyte cells, contributing to their internal structural integrity. Sox10 and CD117 identify the four chromatophore types, with Melan-A specifically detecting only melanocytes and melanophores and not labeling xanthophores and iridophores. Tom20 is used to detect mitochondrial distribution and intensity in the skin, revealing a high density of mitochondria in all epidermal layers, especially in melanocytes and melanophores, compared to xanthophores and iridophores. Numerous telocytes, spindle-shaped with extensions called telopods, are detected in the dermis using CD34, PDGFRα, and vimentin. The skin of the red-eared slider also shows abundant myofibroblasts and well-developed vascularization, with numerous blood vessels detected using α-SMA. This novel study offers an in-depth examination of the limb skin of the red-eared slider through the use of 10 distinct antibodies, uncovering the intricate interactions among its cellular components and providing valuable insights into its anatomical structure and physiological adaptations. Our findings contribute to a better understanding of the turtle's skin, which may aid in its conservation and management.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Electrochemical Performance of Highly Porous CeO₂-Doped Zr Nanoparticles for Supercapacitor Applications.","authors":"M V Arularasu, T V Rajendran, Bassim Arkook, Moussab Harb, K Kaviyarasu","doi":"10.1002/jemt.24728","DOIUrl":"https://doi.org/10.1002/jemt.24728","url":null,"abstract":"<p><p>The aim of this work was to develop an ultrasonic-assisted synthesis method for the fabrication of CeO₂-doped Zr nanoparticles that would improve the performance of supercapacitor electrodes. This method, which eliminates the need for high-temperature calcination, involves embedding CeO₂ into Zr nanoparticles through 1 hr (CeO₂-Zr-1) and 2 hrs (CeO₂-Zr-2) of ultrasonic irradiation, resulting in the formation of nanostructures with significant improvements in their electrochemical properties. Through physicochemical analysis, we observed that the CeO₂-doped Zr nanoparticles, particularly those treated for 2 hrs (CeO₂-Zr-2), exhibit superior crystalline phase purity, optimal chemical surface composition, minimal agglomeration with particle sizes below 50 nm, and an impressive average surface area of 178 m²/g. Compared to the 1 hr irradiation samples (CeO₂-Zr-1) and undoped CeO₂ nanoparticles, the (CeO₂-Zr-2) electrodes demonstrated a remarkable capacitance of 198 Fg^-1 at a current density of 1 A/g while maintaining ~94.9% of their capacity after 3750 cycles. This indicates not only good reversibility but also exceptional stability. In (CeO₂-Zr-2) samples, the nanospherical structure achieved through ultrasonic synthesis is responsible for the enhanced capacitive behavior and stability, along with the synergistic effects caused by Zr doping, which improves the CeO₂ nanoparticle conductivity to a significant extent. Surface areas of the electrodes are larger due to the combination of these two materials, which contribute to their superior performance.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taxonomic Identification of Ligustrum foliosum Nakai: A Comprehensive Study of Macro- and Micromorphology.","authors":"Wajid Zaman, Eun Mi Lee, SeonJoo Park","doi":"10.1002/jemt.24723","DOIUrl":"https://doi.org/10.1002/jemt.24723","url":null,"abstract":"<p><p>Ligustrum foliosum Nakai, a species endemic to South Korea, is a member of the Oleaceae family. This study provides a comprehensive taxonomic identification of L. foliosum based on its macro- and micromorphological characteristics, particularly focusing on the leaf foliar epidermal taxonomy. Scanning electron microscopy (SEM) was used to analyze the leaf structures of L. foliosum collected from various populations on Ulleungdo Island, its primary habitat. Macromorphological assessment revealed that L. foliosum exhibits ovate to elliptical leaves with pointed apices, rounded bases, and entire, smooth margins. The leaves measured 3-10 cm in length and 1.5-4 cm in width, with a pinnate venation pattern and prominent secondary veins. The adaxial surface was dark green and glabrous, while the adaxial surface was lighter green and slightly pubescent. Micromorphological analysis using SEM provided detailed insights into the leaf epidermal characteristics. The epidermal cells were polygonal in shape with straight to slightly curved anticlinal walls. Anomocytic stomata that were not surrounded by specialized cells are the predominant type, with a density ranging from 100 to 150/mm². Epidermal cells measured approximately 10-20 μm in length and 5-10 μm in width, while the stomata typically measured approximately 20-30 μm in length and 10-15 μm in width. Thick-walled bundle sheath cells were evident around the vascular bundles, with a density of 1-3 bundles/mm. The cuticle was moderately thick, providing protection against desiccation, and trichomes were elongated single cell on the leaf both surface. This study contributes to the understanding of L. foliosum's taxonomic position within the Oleaceae family by providing a detailed description of its macro- and micromorphological characteristics. These findings can aid in the identification and conservation of this endemic species, which is crucial for preserving the unique biodiversity of Ulleungdo Island and South Korea.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Anatomical, Histochemical, and Phytochemical Characterization of Species of the Genus Cucumis L.","authors":"Elizandra Maria da Silva, Cledson Dos Santos Magalhães, Karina Perrelli Randau","doi":"10.1002/jemt.24731","DOIUrl":"https://doi.org/10.1002/jemt.24731","url":null,"abstract":"<p><p>Cucumis anguria L. and Cucumis dipsaceus Ehrenb. ex Spach belong to the Cucurbitaceae family and are popularly known as \"maxixe.\" In folk medicine, they are used to treat pneumonia, hyperglycemia, wounds, and malaria. This study aims to characterize the anatomy, histochemistry, and phytochemistry of C. anguria and C. dipsaceus. Conventional methods in plant anatomy and microscopy were used to prepare and analyze semi-permanent slides containing cross-sections of the stem, petiole, leaves, and paradermal sections of the leaf blade. For the histochemical analysis, different reagents were used according to the target metabolite. Phytochemical tests of methanolic extracts of leaves were performed by thin-layer chromatography. The microscopic analysis allowed obtaining the differentiation of plant cells, the presence of tector and glandular trichomes, in addition to showing anatomical traits such as stem shape, petiole shape, and organization of the mesophyll. Histochemistry showed the presence of alkaloids, starch, phenolic compounds, lipophilic compounds, lignin, and tannins. Phytochemical prospection identified monoterpenes, sesquiterpenes, triterpenes, steroids, alkaloids, and reducing sugars. The obtained results provide important information for quality control and species differentiation since there are few studies in the literature on these species.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physio-Mechanic and Microscopic Analyses of Bioactive Glass-Based Resin Infiltrants.","authors":"Syed Zubairuddin Ahmed, Abdul Samad Khan, Wejdan Waleed Nasser, Methayel Abdulrahman Alrushaid, Zahrah Mohammed Alfaraj, Moayad Mohammed Aljeshi, Asma Tufail Shah, Budi Aslinie Md Sabri, Sultan Akhtar, Mohamed Ibrahim Abu Hassan","doi":"10.1002/jemt.24725","DOIUrl":"https://doi.org/10.1002/jemt.24725","url":null,"abstract":"<p><p>This study aimed to investigate the efficacy and durability of bioactive glass-based dental resin infiltrants. Resin infiltrants were formulated by combining photoinitiated dimethacrylate monomers with three variations of bioactive glass: 45S5 Bioglass (RIS), boron-substituted (RIB), fluoride-substituted (RIF), and pure resins (PR), whereby TOOTH group (TH) and ICON (CN) served as commercial control groups. Teeth samples were prepared, and experimental and control infiltrants were applied on demineralized human-extracted teeth. All the samples were subjected to immersion in artificial saliva and pH cycling for 30 days. The samples from another group underwent tooth brushing simulation for 9600 cycles. Following artificial saliva immersion, the samples' hardness values showed that RIB had the highest values (318.44 ± 3.83) while PR (212.52 ± 9.02) had the lowest values. After immersing into the pH cycling solution, the RIF showed the highest hardness (286.86 ± 5.11), while the lowest values for the CN (143.76 ± 3.50). After the tooth brushing simulation, the teeth samples with RIB showed maximum microhardness values (312.06 ± 16.30) and the weakest for the TH (189.60 ± 6.43). The commercial and experimental enamel resin infiltrants showed almost similar results overall, with RIB demonstrating better microhardness and comparable surface roughness. In contrast, RIF proved more resistant to pH cycling, exhibited higher microhardness, and performed better in surface roughness analysis. These findings suggest that resin infiltrant materials, especially RIF, have promising potential for effectively and esthetically managing white spot lesions.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollen and Leaf Micromorphological Characteristics of Spiny Almonds (Prunus subgenus Amygdalus) in Iran.","authors":"Nastaran Bayat, Farideh Attar, Arash Sotoodeh","doi":"10.1002/jemt.24717","DOIUrl":"https://doi.org/10.1002/jemt.24717","url":null,"abstract":"<p><p>This study investigates the micromorphological characteristics of pollen grains and leaf epidermal cells from 20 accessions across four species of spiny almonds using scanning electron microscopy. Thirteen quantitative traits of pollen grains, including exine sculpturing, were analyzed alongside qualitative features such as shape, exine sculpture type, and aperture type. Additionally, four quantitative and five qualitative features of the leaf epidermis were examined, focusing on cuticular ornamentation patterns, types of epicuticular wax, and stomatal measurements. The pollen grains were found to be isopolar monads, radially symmetric, medium-sized, varying from prolate spheroidal to prolate, and exhibiting 3-colporate to 3-colpate structures. The exine sculpturing was generally striate with short and long ridges, with or without perforations, and could be classified into three types. Notably, in the Isfahan population of Prunus lycioides and the North Khorasan population of Prunus spinosissima, the exine sculpture types were distinctly different, being rugulate and reticulate, respectively. Multivariate statistical analysis identified equatorial diameter, colpus length and width, and ridge width as key diagnostic markers for species identification within spiny almonds. Principal component analysis and hierarchical clustering further highlighted the significance of stomatal length, cuticular ornamentation patterns, and epicuticular wax types in differentiating among taxa. We conclude that groupings recognized in recent classifications of the subgenus Amygdalus (spiny almonds) remain challenging to delineate solely based on palynological data, as diverse pollen types are present across different clades and subclades. Furthermore, micromorphological leaf traits proved valuable in distinguishing certain spiny almond taxa, and the traits of epidermal cells may reflect the ecological adaptations of spiny almond species.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142576585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E. coli-Assisted Eco-Friendly Production of Biogenic Silver Cobalt Oxide (AgCoO₂) Nanoparticles: Methanolysis-Based Hydrogen Production, Wastewater Remediation, and Pathogen Control.","authors":"Aisha Irshad, Sajid Mahmood, Tanzeela Fazal, Shahid Iqbal, Mujaddad-Ur- Rehman, Ammar Zidan, Mazloom Shah, Ali Bahadur, Azam Hayat, Asma Khan, Attiya Abdul Malik, Nasser S Awwad, Hala A Ibrahium","doi":"10.1002/jemt.24718","DOIUrl":"https://doi.org/10.1002/jemt.24718","url":null,"abstract":"<p><p>Herein, bacterial-assisted synthesis of AgCoO₂ is carried out. In the first step, E. coli was separated from soil samples via the \"serial dilution method.\" Ten milliliters of bacterial supernatant was mixed with cobalt chloride and silver nitrate hatched at 38°C for 24 h to get AgCoO₂ nanoparticles (NPs). XRD results confirm the synthesis of AgCoO₂ NPs while EDX results confirm the absence of any other elements than Ag, Co, and O. An average NP size of 12-26 nm was determined by TEM examination, and the surface of the particles was seen rough, irregularly shaped borders. The antibacterial activity of the constructed NPs was checked against S. aureus, E. coli, Bacillus subtilus, and Pseudomanas areguinosa using agar well diffusion method. The maximum zone of inhibition was 27 mm at 40 mg/mL against Bacillus subtilus. The performance of the synthesized NPs as photocatalysts was also assessed, and several operational parameters that control the photodegradation of the harmful dyes were tried to tune as well, and 85% degrading efficiency was obtained at 60^oC for 240 min for 30 mg of catalyst dose These NPs were also used to produce hydrogen by methanolysis.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}