Microscopy Research and Technique最新文献

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Interfacial Microstructure and Mechanical Performance of Stainless Steel-Low Alloy Steel Bimetallic Structures Fabricated by Wire Arc Additive Manufacturing. 电弧增材制造不锈钢-低合金钢双金属结构的界面组织与力学性能
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-09-02 DOI: 10.1002/jemt.70177
Prasanna Nagasai Bellamkonda, Maheshwar Dwivedy, Vinayak Kalluri
{"title":"Interfacial Microstructure and Mechanical Performance of Stainless Steel-Low Alloy Steel Bimetallic Structures Fabricated by Wire Arc Additive Manufacturing.","authors":"Prasanna Nagasai Bellamkonda, Maheshwar Dwivedy, Vinayak Kalluri","doi":"10.1002/jemt.70177","DOIUrl":"https://doi.org/10.1002/jemt.70177","url":null,"abstract":"<p><p>Bimetallic structures (BMSs) are being used more frequently in industries like automotive, aerospace, and shipbuilding because they combine different material properties in a single component. Traditional methods like welding and brazing have their limits, but additive manufacturing (AM) offers a more flexible and cost-effective way to create complex multimaterial parts. This study focuses on the microstructure and mechanical properties of BMSs made by depositing stainless steel (SS) on high-strength low-alloy (LA) steel using the Wire Arc Additive Manufacturing (WAAM) process with Surface Tension Transfer (STT). The results show that defect-free BMSs can be successfully fabricated, improving design possibilities. Microstructural analysis revealed a clear Ferrite-Austenite (FA) boundary at the SS-LA interface. Key findings include an 81.81% increase in hardness at the interface relative to the LA steel side, along with an interface tensile strength of 621 ± 7 MPa and elongation of 19.28% ± 1.76%. Electron backscatter diffraction (EBSD) showed fine, randomly oriented grains in the SS region and elongated, directionally aligned grains in the LA region, with no dominant texture, indicating near-isotropic behavior. The presence of both low-angle and high-angle grain boundaries confirmed dynamic recrystallization during deposition. Fracture analysis showed strong mechanical bonding, with failure mainly occurring on the LA side. This demonstrates that WAAM with STT is a viable method for creating reliable, high-performance multimaterial components.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148874184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus) 安纳托利亚野猪舌的宏观、组织学和超微结构特征。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-09-02 Epub Date: 2026-05-07 DOI: 10.1002/jemt.70155
Fatma Işbilir, Banu Kandil, Muhammed Zahid Atli, İhsan Işbilir, Barış Can Güzel
{"title":"Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus)","authors":"Fatma Işbilir,&nbsp;Banu Kandil,&nbsp;Muhammed Zahid Atli,&nbsp;İhsan Işbilir,&nbsp;Barış Can Güzel","doi":"10.1002/jemt.70155","DOIUrl":"10.1002/jemt.70155","url":null,"abstract":"<p>This study provides a detailed characterization of the macroscopic anatomy and microscopic features (light and scanning electron microscopy) of the lingual papillae in the adult Anatolian wild boar (<i>Sus scrofa libycus</i>). Tissue samples were taken from two adult wild boars found dead in their natural habitat within an average of 1.5 h. Following macroscopic observation, samples underwent routine histological processing, histochemical staining, and high-resolution surface morphology analysis via scanning electron microscopy (SEM). Morphological analysis revealed five distinct types of papillae: filiform, fungiform, conical, foliate, and vallate. Filiform papillae, exhibiting either sharp or blunt apices, showed structural adaptations specialized for mechanical function. Fungiform papillae were identified in circular and oval forms; notably, taste buds were restricted to the circular type. Foliate papillae, comprising 3–5 distinct folds, lacked taste buds. A pair of vallate papillae, each demarcated by a prominent trench and housing lateral taste buds, was observed on the caudal tongue. Histochemical evaluation using Periodic Acid-Schiff (PAS) and Alcian Blue (AB) confirmed the presence of both neutral and acidic mucins within the lingual glands. These findings suggest that the lingual morphology of the Anatolian wild boar is closely adapted to its specific dietary habits and ecological niche. This data serves as a critical baseline for comparative anatomy, forensic veterinary medicine, and future morphological research.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 10","pages":"1244-1255"},"PeriodicalIF":2.1,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.70155","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae) 棉粉棉铃虫生殖解剖及卵的超微结构研究(鳞翅目:棉铃虫科)。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-09-02 Epub Date: 2026-05-05 DOI: 10.1002/jemt.70154
K. Logeswaran, Suresh R. Jambagi, Vinithashri Gautam, T. Venkatesan, D. Sagar, S. N. Sushil, M. Mohan
{"title":"Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae)","authors":"K. Logeswaran,&nbsp;Suresh R. Jambagi,&nbsp;Vinithashri Gautam,&nbsp;T. Venkatesan,&nbsp;D. Sagar,&nbsp;S. N. Sushil,&nbsp;M. Mohan","doi":"10.1002/jemt.70154","DOIUrl":"10.1002/jemt.70154","url":null,"abstract":"<div>\u0000 \u0000 <p>The pink bollworm, <i>Pectinophora gossypiella</i> Saunders is a major pest of cotton, notorious for its high reproductive potential and rapid evolution of resistance to <i>Bacillus thuringiensis</i> (Bt) toxins. Despite its economic significance, detailed knowledge of its reproductive anatomy and egg ultrastructure has remained limited, constraining the development of advanced molecular control strategies such as CRISPR/Cas9-based genome editing. The present study provides the first comprehensive characterization of the reproductive system and egg surface morphology of <i>P. gossypiella</i> using stereomicroscopy and scanning electron microscopy (SEM) techniques. The male reproductive system consists of fused, bean-shaped testes, seminal vesicles, duplex and simplex ejaculatory ducts, and paired accessory glands. The female reproductive system comprises paired ovaries with four polytrophic ovarioles per ovary, lateral and common oviducts, accessory glands, corpus bursae, and spermathecal glands. Eggs are oval, dorsoventrally flattened, exhibit a reticulated chorion with distinct micropylar and aeropylar regions. SEM images revealed 6–9 rosette cells encircling a circular micropylar plate, 14–19 first order and 17–23 s order ribs, and 250–291 polygonal surface cells. The structural features of <i>P. gossypiella</i> eggs reveal key sites for sperm entry, aeropylar respiration, and candidate zones for microinjection in gene editing applications. These findings establish a morphological baseline critical for optimizing embryo manipulation and ribonucleoprotein (RNP) delivery in lepidopteran genome editing. This study represents a pioneering effort to integrate classical egg morphology with molecular entomology, thereby advancing precision genetic interventions aimed at resistance management and population suppression in <i>P. gossypiella</i>.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 10","pages":"1218-1229"},"PeriodicalIF":2.1,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite 人工文石近表面相变成核的原位透射电镜观察。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-09-02 Epub Date: 2026-06-10 DOI: 10.1002/jemt.70160
Lehua Wang, Xinshuang Guo, Yuanyuan Zhang, Chaochao Fu, Xingyuan San
{"title":"In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite","authors":"Lehua Wang,&nbsp;Xinshuang Guo,&nbsp;Yuanyuan Zhang,&nbsp;Chaochao Fu,&nbsp;Xingyuan San","doi":"10.1002/jemt.70160","DOIUrl":"10.1002/jemt.70160","url":null,"abstract":"<div>\u0000 \u0000 <p>Electron beam irradiation is well known to induce damage to materials during transmission electron microscope (TEM) characterization, especially for insulating materials. Phase transformation is an important damage phenomenon caused by beam irradiation. Here, we employed in situ TEM combined with electron-energy loss spectroscopy (EELS) to dynamically track the phase transformation of synthetic aragonite. The evolution process and underlying mechanism of the phase transformation in aragonite are systematically discussed. This work reveals that the near-surface region of aragonite undergoes a phase transformation induced by electron beam irradiation, prior to the bulk of aragonite. It provides more possibilities for searching the multiple near-surface properties of materials.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 10","pages":"1256-1265"},"PeriodicalIF":2.1,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148211883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland 瑞士报春花属植物的形态解剖和HPTLC研究。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-09-02 Epub Date: 2026-05-07 DOI: 10.1002/jemt.70153
Carolina Sabedotti, João Vitor da Costa Batista, Bettina Leonhard, Konrad Urech, Mahsa Ahmadinezhad, Jakob Maier, Stephan Baumgartner, Fabio Boylan, Carla Holandino, Jane Manfron
{"title":"Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland","authors":"Carolina Sabedotti,&nbsp;João Vitor da Costa Batista,&nbsp;Bettina Leonhard,&nbsp;Konrad Urech,&nbsp;Mahsa Ahmadinezhad,&nbsp;Jakob Maier,&nbsp;Stephan Baumgartner,&nbsp;Fabio Boylan,&nbsp;Carla Holandino,&nbsp;Jane Manfron","doi":"10.1002/jemt.70153","DOIUrl":"10.1002/jemt.70153","url":null,"abstract":"<p>Primulaceae are distributed all over the world, encompassing around 2590 species. The genus <i>Lysimachia</i> is also part of this family. <i>Lysimachia nummularia</i> L., popularly known as moneywort or creeping Jenny, has been used in Europe to treat many diseases. However, there are no reports of a complete morpho-anatomical description for this species. The aim of this study was to characterize the morpho-anatomical features of the plant, as well as to establish High-Performance Thin-Layer Chromatography (HPTLC)-fingerprints of extracts prepared from the plant by decoction and infusion as well as the organic fractions obtained from these extracts. Standard techniques of light and scanning electron microscopy, as well as histochemical tests, were used; a standard method of HPTLC was applied. The species shows simple, opposite, membranaceous leaves, with rounded roots containing a central medulla and a ribbed stem with endoderm. Amphistomatic leaves bear glandular trichomes on the abaxial surface, also found on the petiole, midrib, petal, and filament. Histochemical analyses confirmed the presence of lipophilic compounds, proteins, starch, phenolics, tannins, and lignin. HPTLC analysis showed some differences in the fingerprints of the organic fractions examined, yet it highlighted the need for further studies regarding the chemical composition of the plant.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 10","pages":"1230-1243"},"PeriodicalIF":2.1,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.70153","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Robust Method for Microscopic 3D Shape Restoration via Shape-From-Focus. 一种基于聚焦形状的微观三维形状恢复方法。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-08-21 DOI: 10.1002/jemt.70167
Yuezong Wang, Yu Niu, Jiqiang Chen
{"title":"A Robust Method for Microscopic 3D Shape Restoration via Shape-From-Focus.","authors":"Yuezong Wang, Yu Niu, Jiqiang Chen","doi":"10.1002/jemt.70167","DOIUrl":"https://doi.org/10.1002/jemt.70167","url":null,"abstract":"<p><p>Shape-from-focus (SFF) is essential for microscopic three-dimensional morphology analysis due to its non-contact nature and high axial resolution. However, in practical microscopy environments, ambient vibration and multi-source noise often cause unstable sampling and distorted focal volume, significantly limiting the technique's applicability in high-precision measurement. To address this, we propose a novel anti-vibration, high-fidelity microscopic SFF framework. First, a temporal redundancy strategy based on video streaming is employed to generate spatially consistent image sequences prior to reconstruction. Subsequently, an adaptive dual-mode peak localization algorithm is introduced, which intelligently selects depth estimation strategies according to the texture characteristics of the micro-surface, achieving sub-pixel accuracy while maintaining computational efficiency. Furthermore, by transforming one-dimensional focus signals into two-dimensional images and applying an intelligent classifier for filtering, the method successfully separates noise from valid texture features. This framework requires no prior knowledge of vibration frequency and adaptively suppresses random environmental disturbances. Experiments on both synthetic and real microscopic samples show that the proposed method outperforms existing approaches in shape recovery accuracy, preservation of fine texture details, and noise suppression. The technique offers a reliable high-precision 3D measurement solution for materials science, industrial inspection, and biomedical microscopy in vibration-prone environments.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148795509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cross-Species Generalization and Comparative Performance Analysis of Deep Neural Network Architectures in Histological Image Classification. 深度神经网络结构在组织学图像分类中的跨物种泛化及性能比较分析。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-08-16 DOI: 10.1002/jemt.70173
Kaan Celen, Fatih Oltulu, Buse Caglar, Aylin Buhur, Burak Kilicarslan, Burhan Berk Çolak, Altug Yavasoglu
{"title":"Cross-Species Generalization and Comparative Performance Analysis of Deep Neural Network Architectures in Histological Image Classification.","authors":"Kaan Celen, Fatih Oltulu, Buse Caglar, Aylin Buhur, Burak Kilicarslan, Burhan Berk Çolak, Altug Yavasoglu","doi":"10.1002/jemt.70173","DOIUrl":"https://doi.org/10.1002/jemt.70173","url":null,"abstract":"<p><p>Histological image classification plays a critical role in biomedical research and diagnostic processes. Advances in the field of deep learning present significant opportunities for enhancing diagnostic accuracy and developing automated decision support systems. This study aims to comparatively evaluate the out-of-distribution generalization and cross-domain classification performance of different deep neural network encoders. In this study, models were trained on an internal dataset of 4307 hematoxylin and eosin (H&E) stained images of male rat lung, cerebellum, and adipose tissues, obtained under ethical committee approval (Ege University, HADYEK 2026-06). To evaluate genuine generalization, testing was conducted on a separate, diverse external cohort of 600 mixed human-and-animal images. Nine different deep learning architectures-MobileNetV3-S, MobileNetV3-L, DenseNet121, DenseNet201, ConvNeXt-S, ConvNeXt-B, DINOv3 ViT-S, DINOv3 ViT-H+, and the pathology foundation model UNI2-h-were evaluated using frozen feature extraction combined with a linear probe. For performance evaluation, accuracy, sensitivity, specificity, F1 score, Cohen's κ, and ROC-AUC metrics were analyzed along with per-image inference times. While all models achieved near-perfect results during internal cross-validation, their performance diverged significantly on the external dataset, confirming that the observed differences reflect genuine cross-domain generalization capabilities rather than under-fitting. All competitive models demonstrated high performance in classifying adipose and cerebellum tissues, achieving an F1 score of 95% or higher for these tissues. In distinguishing the lung tissue, which has the most diverse structure and is the most difficult to classify, the UNI2-h model emerged as the most successful, achieving an F1 score of 97.4% and a recall of 95.0%. When evaluated in terms of computational efficiency, the MobileNetV3-Small model stood out as having the lowest processing time among all scenarios, demonstrating an inference time of 9.4 ms on the CPU and 12.5 ms on the GPU. In conclusion, while the pathology foundation model UNI2-h achieved the highest accuracy values across all metrics, the ConvNeXt-Small model was identified as the system providing the optimal balance between speed and accuracy. These findings indicate that although UNI2-h stands out for the most precise results in cross-domain classifications, the ConvNeXt-Small architecture emerges as an ideal alternative, particularly in practical applications where computational efficiency is critical and resources are constrained.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148766267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preparation of Oxide Nanofibers for Analysis by Transmission Electron Microscopy. 用于透射电镜分析的氧化纳米纤维的制备。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-08-16 DOI: 10.1002/jemt.70174
Søren Bredmose Simonsen, Waynah Lou Dacayan, Zhongtao Ma, Kristian Speranza Mølhave, Elisabeth Agnes Müller Gubler, Wenjing Zhang
{"title":"Preparation of Oxide Nanofibers for Analysis by Transmission Electron Microscopy.","authors":"Søren Bredmose Simonsen, Waynah Lou Dacayan, Zhongtao Ma, Kristian Speranza Mølhave, Elisabeth Agnes Müller Gubler, Wenjing Zhang","doi":"10.1002/jemt.70174","DOIUrl":"https://doi.org/10.1002/jemt.70174","url":null,"abstract":"<p><p>The low electronic conductivity and rigid structure make sample preparation for in situ transmission electron microscopy (TEM) of oxide nanofibers challenging. This work evaluates three sample preparation approaches for mounting single oxide nanofibers on MEMS chips: (i) drop casting combined with focused ion beam (FIB) cutting and metal deposition, (ii) single-probe micromanipulation, and (iii) double-probe micromanipulation inside a FIB-SEM. Advantages and limitations of each method are discussed, including issues of fiber agglomeration, positioning/orientation, and charging in the electron or ion beam. Drop casting offers simplicity but poor positional control. The probe-based methods enable precise positioning; charging however remains a major challenge which is best compensated for by using more than one microprobe. Strategies for improving electrical contact and minimizing contamination during metal deposition are also addressed. The findings provide practical guidelines for preparing oxide nanofibers for operando TEM experiments involving electrical and thermal stimuli.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148761906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation on the Stress Relaxation Property of 2A97 Al-Li Alloy Assisted by Electric Pulse 电脉冲辅助下2A97铝锂合金应力松弛性能的研究。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-08-08 Epub Date: 2026-03-28 DOI: 10.1002/jemt.70143
Peng Zhang, Zhiyang He, Jian Ning, Qingpeng Ding, Rui Zhang, Zuyan He
{"title":"Investigation on the Stress Relaxation Property of 2A97 Al-Li Alloy Assisted by Electric Pulse","authors":"Peng Zhang,&nbsp;Zhiyang He,&nbsp;Jian Ning,&nbsp;Qingpeng Ding,&nbsp;Rui Zhang,&nbsp;Zuyan He","doi":"10.1002/jemt.70143","DOIUrl":"10.1002/jemt.70143","url":null,"abstract":"<div>\u0000 \u0000 <p>The influence of the strain on the formability of 2A97-T3 Al–Li alloy during electric pulse-assisted forming was investigated in the research. Electrically assisted stress relaxation tests were conducted, and a corresponding stress relaxation model was developed and validated. The mechanism of the pulsed current influences stress relaxation in the alloy was revealed based on the analysis of stress relaxation curves and microstructure. Optimal processing performance for the 2A97-T3 Al-Li alloy was obtained at 450 A, 12 ms pulse width, 60% duty cycle, and 50 Hz frequency. A yield strength of 89 MPa and an elongation of 13.2% were obtained under the conditions. Furthermore, a residual stress of only 0.142 MPa was recorded after relaxation at <i>ε</i> = 0.0667. Under combined the strain and the Joule heating, the grains were refined by dynamic recovery and recrystallization. Grains first coarsened and then they were elongated, while the proportion of low-angle grain boundaries increased concurrently. Concurrently, the &lt;101&gt; texture strength increased markedly. Its maximum intensity rose from 2.225 to 4.755, which was attributed to the mechanism of continuous dynamic recrystallization. It was demonstrated that the stress was effectively reduced and the formability of the alloy was significantly improved by means of electric pulse-assisted forming.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 9","pages":"1185-1194"},"PeriodicalIF":2.1,"publicationDate":"2026-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147530994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural Modifications in the Adhesive Organ of a Hillstream Catfish Hara hara : Scanning Electron Microscopy and Histochemistry 山溪鲶鱼粘附器官的结构修饰:扫描电镜和组织化学。
IF 2.1 3区 工程技术
Microscopy Research and Technique Pub Date : 2026-08-08 Epub Date: 2026-04-16 DOI: 10.1002/jemt.70148
Jitendra Kumar Sharma, Usha Kumari, Swati Mittal, Ajay Kumar Mittal
{"title":"Structural Modifications in the Adhesive Organ of a Hillstream Catfish Hara hara : Scanning Electron Microscopy and Histochemistry","authors":"Jitendra Kumar Sharma,&nbsp;Usha Kumari,&nbsp;Swati Mittal,&nbsp;Ajay Kumar Mittal","doi":"10.1002/jemt.70148","DOIUrl":"10.1002/jemt.70148","url":null,"abstract":"<div>\u0000 \u0000 <p>In the present study, the adhesive organ (AO) of \u0000 <i>Hara hara</i>\u0000 , a hillstream catfish, has been investigated using scanning electron microscopy, histology, and glycoprotein, protein, and lectin histochemistry. The AO is a disc-like structure present on the ventral side of the body. It is distinguished into a major flat central region and a narrow flap-like region at the periphery of the central region. The free surface of the AO is characteristically differentiated into plaques separated by furrows. Surface epithelial cells in the plaques are modified into unicellular spine-like unculi. The furrow epithelium contains three types of unicellular gland cells (the mucous goblet cells, the club cells, and the sacciform cells), along with sensory structures such as the taste buds (TBs). Gland cells are involved in the elaboration of various glycoproteins (oxidizable vicinal diols, carboxyl groups, and O-sulphate esters); protein moieties (basic proteins, cysteine-bound sulphydryl groups, keratin, tyrosine, protein bound-NH<sub>2</sub> groups, and elastin); and glycans (galactose, N-Acetylgalactosamine, and fucose). Presence of these glycoprotein and protein moieties elaborated by the cellular component of the AO has been associated to play an important role in maintaining structural integrity of the epithelium, keratinization, protection against pathogens, and adhesion of the fish to the substratum. The TBs located at the summit of the tubercles in the furrow epithelium may serve as an adaptation to enhance sensitivity to food selection and environmental perception. Overall, this study provides a comprehensive understanding of the structural and functional adaptations that enable \u0000 <i>H. hara</i>\u0000 to maintain effective attachment and thrive in turbulent hillstream environments.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 9","pages":"1195-1212"},"PeriodicalIF":2.1,"publicationDate":"2026-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147699181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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