Microscopy Research and Technique最新文献

{"title":"Structural characteristics and regenerative potential: Insights from the molly fish spinal cord","authors":"Mahmoud Awad,&nbsp;Ramy K. A. Sayed,&nbsp;Dalia Mohammadin,&nbsp;Marwa M. Hussein,&nbsp;Doaa M. Mokhtar","doi":"10.1002/jemt.24633","DOIUrl":"10.1002/jemt.24633","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Unlike mammals, species such as fish and amphibians can regenerate damaged spinal cords, offering insights into potential therapeutic targets. This study investigates the structural features of the molly fish spinal cord through light and electron microscopy. The most notable characteristic was the presence of Mauthner cells (M-cells), which exhibited large cell bodies and processes, as well as synaptic connections with astrocytes. These astrocytic connections contained synaptic vesicles, suggesting electrical transmission at the M-cell endings. Astrocytes, which were labeled with glial fibrillary acidic protein (GFAP), contained cytoplasmic glycogen granules, potentially serving as an emergency fuel source. Two types of oligodendrocytes were identified: a small, dark cell and a larger, lighter cell, both of which reacted strongly with oligodendrocyte transcription factor 2 (Olig2). The dark oligodendrocyte resembled human oligodendrocyte precursors, while the light oligodendrocyte was similar to mature human oligodendrocytes. Additionally, proliferative neurons in the substantia grisea centralis expressed myostatin, Nrf2, and Sox9. Collectively, these findings suggest that the molly fish spinal cord has advanced structural features conducive to spinal cord regeneration and could serve as an excellent model for studying central nervous system regeneration. Further studies on the functional aspects of the molly fish spinal cord are recommended.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Mauthner cells (M-cell), with their typical large cell body and processes, were the most characteristic feature in Molly fish spinal cord, where it presented synaptic connections with astrocytes and their ends contained synaptic vesicles indicating an electrical transmission in the M-cells endings.</li>\u0000 \u0000 <li>Two types of oligodendrocytes could be recognized; both reacted intensely with Oligodendrocyte transcription factor 2 (Olig2).</li>\u0000 \u0000 <li>The proliferative neurons of the substantia grisea centralis expressed myostatin, Nrf2, and Sox9.</li>\u0000 \u0000 <li>The findings of this study suggest that molly fish possess highly developed structural features conducive to spinal cord regeneration. Consequently, they could be deemed an exemplary model for investigating central nervous system regeneration.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micromorphological study of some Salsola species (Amaranthaceae) in Iran and its systematic significance using scanning electron microscopy","authors":"Elham Amini,&nbsp;Ali Sattarian,&nbsp;Fatemeh Nasrollahi,&nbsp;Abolfazl Daneshvar,&nbsp;Majid Mohammad Esmaeili,&nbsp;Leila Hamidzadeh Sani,&nbsp;Sona Haghighi","doi":"10.1002/jemt.24623","DOIUrl":"10.1002/jemt.24623","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Using scanning electron microscopy (SEM), six <i>Salsola</i> species from Iran were examined for their epidermis, seed, and fruit micromorphology. Among them were <i>S. brachiata</i> from section <i>Heterotricha</i>, <i>S dendroides</i>, <i>S. incanescens</i>, and <i>S. orientalis</i> from section <i>Caroxylon</i>, <i>S. kali</i> from section <i>Kali</i>, and <i>S. turcomanica</i> from section <i>Physurus</i>. Epidermal cells are divided into three types. There were diamond, irregular, and polygonal cells, as well as straight and undulated walls. Studied species of <i>Salsola</i> have smooth or sculptured fruit surfaces, and there are three main types of fruit surface ornamentation. There is a significant difference between these species based on the type of hair and density of the fruit. Seed shape and color have little systematic significance. The seed epidermis is composed of polygonal, elongated polygonal, irregular, and diamond cells. Although polygonal and irregular testa cells are most common, their size and shape can provide additional information and useful diagnostic characteristics at both specific and infraspecific levels. For taxonomic separation, the current study provides novel insights at micromorphological levels.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>This article reports halophyte are shown as models for adaptation to extreme habitats.</li>\u0000 \u0000 <li>These plants are placed among the ecological communities of xerophytes. Here, for the first time, the microstructural analysis of <i>Salsola</i> has been investigated.</li>\u0000 \u0000 <li>Additionally, it provides new insights into plant species' response to extreme conditions, as well as possible adaptation strategies at the micromorphological level.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of lactobacillus casei and lactobacillus plantarum to develop dried functional apple and banana","authors":"Sahar Sabahi,&nbsp;Seyyed Mohammad Ali Noori,&nbsp;Alireza Ekrami,&nbsp;Seyed Ahmad Hosseini,&nbsp;Maryam Seyedtabib,&nbsp;Sousan Akrami","doi":"10.1002/jemt.24639","DOIUrl":"10.1002/jemt.24639","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>The goal of this research was to create dried fruits loaded with probiotic microorganisms (<i>Lactobacillus casei</i> and <i>Lactobacillus plantarum</i>). In separate bottles for each probiotic microbe, apple and banana pieces have been submerged into the impermeability solution with gentle shaking. The vacuum pressure was applied. By the conclusion of the incubation time, <i>L. casei</i> and <i>L. plantarum</i> colonies were enumerated (CFU/g). The scanning electron microscope method was applied to confirm the penetration of impregnation solutions into the intercellular spaces of fruit tissue. On day 28, the population of <i>L. plantarum</i> was 5 log CFU/g for apples and 5.5 log CFU/g for bananas. After storage, the number of <i>L. casei</i> in apples was 5 log CFU/g and 5.5 log CFU/g, respectively. <i>L. casei</i> was found on the surface of apple and banana tissue. After one-week, whole phenolic content of probiotic-enriched bananas and apples augmented. After storage, the antioxidant activity of all samples decreased greatly. The sensory qualities of the samples were excellent throughout storage in terms of color, quality, scent, sensitivity, chewiness, and general adequacy. As a result, dried apples and bananas infused with <i>L. plantarum</i> and <i>L. casei</i> might be a novel probiotic meal.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Dried apples and bananas infused with <i>L. plantarum</i> and <i>L. case</i>i are novel probiotic meal.</li>\u0000 \u0000 <li>After one-week, whole phenolic content of probiotic-enriched bananas and apples augmented.</li>\u0000 \u0000 <li>The sensory qualities of the samples were excellent throughout storage in terms of color, quality, scent, sensitivity, chewiness, and general adequacy.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomy and micromorphology of Tilia species from Turkey and its taxonomic implication","authors":"Beste Gökhan Strelnikov,&nbsp;Bahar Gürdal","doi":"10.1002/jemt.24634","DOIUrl":"10.1002/jemt.24634","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>The genus <i>Tilia</i> L. belongs to Malvaceae and it is represented by four species in Turkey. These are <i>T. cordata</i>, <i>T. platyphyllos</i>, <i>T. rubra</i> subsp. <i>caucasica</i>, and <i>T. tomentosa</i>. <i>Tilia</i> species have medicinal importance, especially to treat colds, flu, and coughs, and are also used as sedatives. Inflorescence with bract is recorded in the pharmacopeia as used parts. This study aimed to investigate the differences in leaf and bract characteristics in distinguishing <i>Tilia</i> species. Leaves and bracts were examined anatomically and micromorphologically under light and scanning electron microscopes (LM and SEM). There is no comparative anatomical and micromorphological study of <i>Tilia</i> species from Turkey. Microscopic examination allows for the observation of stomata types, trichomes, and other anatomical structures. These features can vary among species and can aid in differentiation. Features of bract that is medicinally used part were evaluated to find efficient diagnostic characters. The leaves also possess distinct characteristics, and leaf morphology is already used in the identification key in Flora. The emphasis is on identifying the species of medically used drugs. A diagnostic key was created using LM and SEM based on bract features to differentiate taxa for the first time.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Leaf and bract anatomy of <i>Tilia</i> taxa was studied via LM and SEM.</li>\u0000 \u0000 <li>The raw plant material is inflorescence with bract so identification by bract is important for species adulteration.</li>\u0000 \u0000 <li>An diagnostic key based on bract features was created for the first time.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.24634","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141458102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing tick diversity: Chemical profiling and dynamics in scanning microscopy and molecular phylogenetics","authors":"Ayesha Malik,&nbsp;Kiran Afshan,&nbsp;Mohammad K. Okla,&nbsp;Ibrahim A. Saleh,&nbsp;Abdul Razzaq,&nbsp;Munib Hussain,&nbsp;Sabika Firasat,&nbsp;Erinda Lika,&nbsp;Màrius Vicent Fuentes","doi":"10.1002/jemt.24620","DOIUrl":"10.1002/jemt.24620","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>This study presents a comprehensive investigation into the evolutionary trajectories of <i>Rhipicephalus</i> ticks (Ixodidae) through the interpretation of molecular phylogenetics, elucidating their chromatographic spectrum. The use of advanced chromatographic tools in this study explored the dynamics chemical profiling, providing valuable insights into the evolutionary history and ecological adaptations. Prevalence of <i>Rhipicephalus</i> ticks was 4.5% in sheep and 3.9% in goats. The ITS2 sequence of the <i>Rhipicephalus sanguineus</i> (OK642408) and <i>Rhipicephalus microplus</i> (OK642409) form a distinct clade with sequences from other countries. The 16S rRNA sequences of <i>R. sanguineus</i> (OK560870) clustered with sequences form three lineages, tropical, temperate, and south-eastern. The Cox I gene-identified <i>Rhipicephalus turanicus</i> (OK623472) and <i>R. microplus</i> (OK623463) form separate clades with sequences. The HPLC chromatogram of tick samples reveals a diverse array of identified hydrocarbons, explained the complex chemical composition of their exoskeletons. This analytical approach provides valuable insights into the specific hydrocarbon profiles, allowing for potential applications in species differentiation, ecological studies, and a deeper understanding of the functional roles played by hydrocarbon compounds in tick physiology. The findings revealed the potential of applying molecular phylogenetics tools with chromatography not only to enhance our understanding of tick evolution but also to inform strategies for disease control and management in regions where <i>Rhipicephalus</i> ticks (Ixodidae) are endemic.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Chemical mapping utilizing advanced chromatographic techniques.</li>\u0000 \u0000 <li>Scanning microscopic insights high-resolution scanning tool to observe structural and morphological features of ticks at a molecular level.</li>\u0000 \u0000 <li>Molecular phylogeny data elucidate the evolutionary relationships among tick species.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive block imaging based on compressive sensing in AFM","authors":"Yuchuan Zhang,&nbsp;Yongjian Chen,&nbsp;Teng Wu,&nbsp;Guoqiang Han","doi":"10.1002/jemt.24618","DOIUrl":"10.1002/jemt.24618","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Atomic force microscopy (AFM) is a kind of high-precision instrument to measure the surface morphology of various conductive or nonconductive samples. However, obtaining a high-resolution image with standard AFM scanning requires more time. Using block compressive sensing (BCS) is an effective approach to achieve rapid AFM imaging. But, the routine BCS-AFM imaging is difficult to balance the image quality of each local area. It is easy to lead to excessive sampling in some flat areas, resulting in time-consuming. At the same time, there is a lack of sampling in some areas with significant details, resulting in poor imaging quality. Thus, an innovative adaptive BCS-AFM imaging method is proposed. The overlapped block is used to eliminate blocking artifacts. Characteristic parameters (GTV, <i>L</i>_u, and SD) are used to predict the local morphological characteristics of the samples. Back propagation neural network is employed to acquire the appropriate sampling rate of each sub-block. Sampling points are obtained by pre-scanning and adaptive supplementary scanning. Afterward, all sub-block images are reconstructed using the TVAL3 algorithm. Each sample is capable of achieving uniform, excellent image quality. Image visual effects and evaluation indicators (PSNR and SSIM) are employed for the purpose of evaluating and analyzing the imaging effects of samples. Compared with two nonadaptive and two other adaptive imaging schemes, our proposed scheme has the characteristics of a high degree of automation, uniformly high-quality imaging, and rapid imaging speed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>The proposed adaptive BCS method can address the issues of uneven image quality and slow imaging speed in AFM.</li>\u0000 \u0000 <li>The appropriate sampling rate of each sub-block of the sample can be obtained by BP neural network.</li>\u0000 \u0000 <li>The introduction of GTV, <i>L</i>_u, and SD can effectively reveal the morphological features of AFM images.</li>\u0000 \u0000 <li>Seven samples with different morphology are used to test the performance of the proposed adaptive algorithm.</li>\u0000 \u0000 <li>Practical experiments are carried out with two samples to verify the feasibility of the proposed adaptive algorithm.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141327679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization, anti-microbial, anti-oxidant and growth promoting effects of biogenically synthesized silver nanoparticles derived from Dicliptera bupleuroides Nees","authors":"Memoona Samad,&nbsp;Wasim Akhtar,&nbsp;Arooj Muneer,&nbsp;Iram Fatima,&nbsp;Muhammad Manzoor,&nbsp;Syed Waseem Gillani,&nbsp;Fazal Ur Rehman,&nbsp;Khawar Majeed,&nbsp;Baber Ali,&nbsp;Rizwan Sarwar","doi":"10.1002/jemt.24630","DOIUrl":"10.1002/jemt.24630","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>One of the most important areas of nanotechnology is the use of nanoparticles (NPs) for a variety of environmental and biological applications, with silver nanoparticles (Ag-NPs) gaining a lot attention due to their distinct properties. The current study deals with the synthesis of Ag-NPs from <i>Dicliptera bupleuroides</i> Nees leaf extract and to determine their antioxidant, antimicrobial potential and effects on wheat seed germination and growth. UV–Visible spectrum revealed a prominent absorption peak at 442 nm, elucidating the conformation of the Ag-NPs synthesis. Scanning electron microscopy (SEM) showed distinctive triangular, pyramidal, and irregular shape. X-ray diffraction (XRD) demonstrated their crystalline nature, with average crystallite size of the Ag-NPs measured at 20.52 nm. Fourier-transform infrared spectroscopy (FT-IR) further confirmed the presence of functional groups such as Phenols (O-H stretch), transition metal carbonyls N-H, ≡C-H, C ≡ N, C ≡ C, C-Cl, C-Br and O-H bonds on the surface Ag-NPs. The antibacterial activity of the Ag-NPs was most pronounced against <i>Bacillus subtilis</i>, with a zone of inhibition (ZOI) measuring 11 mm ± 0.57 at a concentration of 1000 μg/mL (45% inhibition). Likewise, Ag-NPs exhibited highest antioxidant potential (73.2%) at 100 μg/mL compared with standard (ascorbic acid) which showed (76%) at the same concentration. Furthermore, the effect of <i>D. bupleuroides</i> mediated Ag-NPs on wheat seeds growth and germination was recorded maximum at high concentrations (200-300 ppm). In conclusion, <i>D. bupleuroides</i> mediated Ag-NPs showed safe, cost effective and environmentally friendly synthesis which can be used as an antibacterial and antioxidant agent as well as for enhancing the growth and seed germination of crop seeds globally.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Nanotechnology is the study of nanoparticles for biological and environmental applications.</li>\u0000 \u0000 <li>Ag-NPs among other NPs have received broad attention because of their unique properties.</li>\u0000 \u0000 <li><i>D. bupleuroides</i> Ag-NPs: 45% antibacterial, 73.2% antioxidant, enhance wheat germination.</li>\u0000 \u0000 <li><i>D. bupleuroides-</i>mediated Ag-NPs are both cost-effective and environmentally beneficial.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141321216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How scanning probe microscopy can be supported by artificial intelligence and quantum computing?","authors":"Agnieszka Pregowska,&nbsp;Agata Roszkiewicz,&nbsp;Magdalena Osial,&nbsp;Michael Giersig","doi":"10.1002/jemt.24629","DOIUrl":"10.1002/jemt.24629","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>The impact of Artificial Intelligence (AI) is rapidly expanding, revolutionizing both science and society. It is applied to practically all areas of life, science, and technology, including materials science, which continuously requires novel tools for effective materials characterization. One of the widely used techniques is scanning probe microscopy (SPM). SPM has fundamentally changed materials engineering, biology, and chemistry by providing tools for atomic-precision surface mapping. Despite its many advantages, it also has some drawbacks, such as long scanning times or the possibility of damaging soft-surface materials. In this paper, we focus on the potential for supporting SPM-based measurements, with an emphasis on the application of AI-based algorithms, especially Machine Learning-based algorithms, as well as quantum computing (QC). It has been found that AI can be helpful in automating experimental processes in routine operations, algorithmically searching for optimal sample regions, and elucidating structure–property relationships. Thus, it contributes to increasing the efficiency and accuracy of optical nanoscopy scanning probes. Moreover, the combination of AI-based algorithms and QC may have enormous potential to enhance the practical application of SPM. The limitations of the AI-QC-based approach were also discussed. Finally, we outline a research path for improving AI-QC-powered SPM.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Artificial intelligence and quantum computing as support for scanning probe microscopy.</li>\u0000 \u0000 <li>The analysis indicates a research gap in the field of scanning probe microscopy.</li>\u0000 \u0000 <li>The research aims to shed light into ai-qc-powered scanning probe microscopy.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.24629","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental morpho-analysis of the caecum in Japanese quail embryos (Coturnix coturnix japonica)","authors":"Fatma Abdelhakeem","doi":"10.1002/jemt.24632","DOIUrl":"10.1002/jemt.24632","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>In the current study, we are focusing on the microanatomical structure of quail caecum during the prehatching time to try to understand the function and the role of each cell-built quail caecum reaching how caecum plays an essential role in immunity and absorption. The morpho-developmental features of the quail caecum were described in detail daily from the third incubation day (ID) till hatching time, investigating the gross morphology, microscopic, and ultrastructure using light and scanning electron microscope. The embryonic caecum appeared grossly as two lateral outpocketings with blinded ends, emerging laterally at the junction between the small and large intestine (the ileocaecal junction). The primordia of two caeca, represented by two lateral swellings from the hindgut on the fourth ID, continued growing till the day of hatching, where the caecal wall consisted of three apparent layers: mucosa, musculosa, and serosa. At the time of hatching, the quail caecum was still not fully mature and will continue growing posthatching. The findings in this study can be applied in further studies intended to understand the physiological mechanisms of the caecum during prehatching and posthatching periods.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>Caecum is one of the hindgut derivatives that started as two lateral swellings.</li>\u0000 \u0000 <li>The caecal wall consisted of three layers; mucosa, musculosa, and serosa.</li>\u0000 \u0000 <li>The caecum plays an essential role in immunity maintenance.</li>\u0000 \u0000 <li>Caecum continues to grow posthatching as it is not fully mature at hatching time.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141306296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An examination of the effect of exercise and creatine monohydrate on oral tissues","authors":"Mehmet Sinan Dogan,&nbsp;Hakim Celik,&nbsp;Sibel Türedi,&nbsp;Abdullah Taskın,&nbsp;Mehmet Emin Dogan,&nbsp;Şemsettin Yıldız","doi":"10.1002/jemt.24626","DOIUrl":"10.1002/jemt.24626","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Although physical exercise is extremely important for health and a good lifestyle, it can trigger oxidative stress, inflammation, and muscle fatigue. The aim of this study was to determine changes in dental tissues and the mandible created by creatines monohydrate (CrM) supplementation together with low and high-intensity exercise (HIE). The study material comprised Balb/c male mices, which were separated into two groups for the application of low and HIE on a running band. CrM supplement was administered together with the exercise. At the end of the experiment period, dental tissue samples were surgically removed and examined histopathologically and immunohistochemically (TNF-<i>α</i> and lL-1<i>β</i>).As a result of the histopathological examinations, in the pulp, oedema, vascular congestion, and capillary dilatation were seen to be statistically significantly increased in the Group 3 mices that performed HIE compared to the control group (<i>p</i> = 0.001, <i>p</i> = 0.003, <i>p</i> = 0.001, respectively). A statistically significant increase was observed in periodontal ligament (PDL) degeneration, and disruption of the continuity and separation of collagen fibers in Group 3 compared to the control group (<i>p</i> = 0.001). In the immunohistochemical examination, TNF-<i>α</i> and IL-1<i>β</i> positivity was observed in Group 3, and this was significantly increased compared to the control group (<i>p</i> = 0.001, <i>p</i> = 0.000).Exposure of the mices to low and HIE caused histological and immunohistochemical changes in dental pulp and PDL, and it was determined that the use of CrM could have a protective effect against these changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 <ul>\u0000 \u0000 <li>The results of this study showed negative effects of HIE in the dental pulp and PDL, which play an important role in dental health.</li>\u0000 \u0000 <li>CrM was seen to be effective in preventing these negative effects.</li>\u0000 </ul>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.24626","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}