Microscopy Research and Technique最新文献

{"title":"Correction to “Microscopic Analysis of Plant-Mediated Silver Nanoparticle Toxicity in Rainbow Trout Fish (Oncorhynchus mykiss)”","authors":"","doi":"10.1002/jemt.24713","DOIUrl":"10.1002/jemt.24713","url":null,"abstract":"<p>Ali, I., Khan, S., Shah, K., et al., (2021). “Microscopic Analysis of Plant-Mediated Silver Nanoparticle Toxicity in Rainbow Trout Fish (<i>Oncorhynchus mykiss</i>).” <i>Microscopy Research and Technique</i>, 84(10), 2302–2310. https://doi.org/10.1002/jemt.23785.</p><p>In the originally-published article, Liujiao Bian was inadvertently included in the author list. Additionally, funding was erroneously attributed to National Natural Science Foundation of China. The online version of this article has been updated to remove the author and the funding information. The corresponding author is now designated as Ihsan Ali.</p><p>We apologize for this error.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"618"},"PeriodicalIF":2.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.24713","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Assay on the Antibactericidal Functionality of Synthesized Mg-Doped ZnO Nanoparticles on the Dental Microbes","authors":"Eman A. Alhomaidi,&nbsp;Ibrahim A. Saleh,&nbsp;Naser Zomot,&nbsp;Jehad S. Al-Hawadi,&nbsp;Mansour Kha","doi":"10.1002/jemt.24720","DOIUrl":"10.1002/jemt.24720","url":null,"abstract":"<div>\u0000 \u0000 <p>We succeeded in producing pure and magnesium-doped zinc oxide nanoparticles (Mg-Zn NPs) by making use of a <i>Prosopis farcta</i> leaf extract and subsequently distinguished the quality of our NPs with the use of field energy scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX), powder X-ray diffraction (PXRD), and UV–vis. In correlation to our observations, the particulates were spherically produced at a size of 20 nm with the ability to cause antimicrobial impacts on <i>Streptococcus mutans</i> bacteria and <i>Candida albicans</i> fungi. Inhibition zones of 18 ± 0.3 and 24 ± 0.3 mm were obtained for 5% Mg-Zn NPs against bacteria and fungi, respectively. Based on these results, our work suggests a practicable proposition for our synthesized product to be considered as a worthy alternative for dental and oral utilizations.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"516-522"},"PeriodicalIF":2.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CellSAM: Advancing Pathologic Image Cell Segmentation via Asymmetric Large-Scale Vision Model Feature Distillation Aggregation Network","authors":"Xiao Ma,&nbsp;Jin Huang,&nbsp;Mengping Long,&nbsp;Xiaoxiao Li,&nbsp;Zhaoyi Ye,&nbsp;Wanting Hu,&nbsp;Yaxiaer Yalikun,&nbsp;Du Wang,&nbsp;Taobo Hu,&nbsp;Liye Mei,&nbsp;Cheng Lei","doi":"10.1002/jemt.24716","DOIUrl":"10.1002/jemt.24716","url":null,"abstract":"<div>\u0000 \u0000 <p>Segment anything model (SAM) has attracted extensive interest as a potent large-scale image segmentation model, with prior efforts adapting it for use in medical imaging. However, the precise segmentation of cell nucleus instances remains a formidable challenge in computational pathology, given substantial morphological variations and the dense clustering of nuclei with unclear boundaries. This study presents an innovative cell segmentation algorithm named CellSAM. CellSAM has the potential to improve the effectiveness and precision of disease identification and therapy planning. As a variant of SAM, CellSAM integrates dual-image encoders and employs techniques such as knowledge distillation and mask fusion. This innovative model exhibits promising capabilities in capturing intricate cell structures and ensuring adaptability in resource-constrained scenarios. The experimental results indicate that this structure effectively enhances the quality and precision of cell segmentation. Remarkably, CellSAM demonstrates outstanding results even with minimal training data. In the evaluation of particular cell segmentation tasks, extensive comparative analyzes show that CellSAM outperforms both general fundamental models and state-of-the-art (SOTA) task-specific models. Comprehensive evaluation metrics yield scores of 0.884, 0.876, and 0.768 for mean accuracy, recall, and precision respectively. Extensive experiments show that CellSAM excels in capturing subtle details and complex structures and is capable of segmenting cells in images accurately. Additionally, CellSAM demonstrates excellent performance on clinical data, indicating its potential for robust applications in treatment planning and disease diagnosis, thereby further improving the efficiency of computer-aided medicine.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"501-515"},"PeriodicalIF":2.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142504266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Accuracy and Lightweight Image Classification Network for Optimizing Lymphoblastic Leukemia Diagnosisy","authors":"Liye Mei,&nbsp;Chentao Lian,&nbsp;Suyang Han,&nbsp;Shuangtong Jin,&nbsp;Jing He,&nbsp;Lan Dong,&nbsp;Hongzhu Wang,&nbsp;Hui Shen,&nbsp;Cheng Lei,&nbsp;Bei Xiong","doi":"10.1002/jemt.24704","DOIUrl":"10.1002/jemt.24704","url":null,"abstract":"<div>\u0000 \u0000 <p>Leukemia is a hematological malignancy that significantly impacts the human immune system. Early detection helps to effectively manage and treat cancer. Although deep learning techniques hold promise for early detection of blood disorders, their effectiveness is often limited by the physical constraints of available datasets and deployed devices. For this investigation, we collect an excellent-quality dataset of 17,826 morphological bone marrow cell images from 85 patients with lymphoproliferative neoplasms. We employ a progressive shrinking approach, which integrates a comprehensive pruning technique across multiple dimensions, including width, depth, resolution, and kernel size, to train our lightweight model. The proposed model achieves rapid identification of acute lymphoblastic leukemia, chronic lymphocytic leukemia, and other bone marrow cell types with an accuracy of 92.51% and a throughput of 111 slides per second, while comprising only 6.4 million parameters. This model significantly contributes to leukemia diagnosis, particularly in the rapid and accurate identification of lymphatic system diseases, and provides potential opportunities to enhance the efficiency and accuracy of medical experts in the diagnosis and treatment of lymphocytic leukemia</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"489-500"},"PeriodicalIF":2.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological Characterization of Culex Quinquefasciatus (Diptera: Culicidae) Pupae in the Eastern Province of Saudi Arabia","authors":"Abeer S. Yamany,&nbsp;Rewaida Abdel-Gaber","doi":"10.1002/jemt.24714","DOIUrl":"10.1002/jemt.24714","url":null,"abstract":"<div>\u0000 \u0000 <p>Over the last 30 years, several mosquito-borne diseases have spread worldwide, threatening human and animal health, including lymphatic filariasis. Lymphatic filariasis is a major public health issue in tropical regions like Saudi Arabia, caused by parasitic worms. The parasitic worms <i>Wuchereria bancrofti</i> and <i>Brugia malayi</i> are transmitted by <i>Culex quinquefasciatus</i>. <i>Cx</i>. <i>quinquefasciatus</i> is a potential vector for various arboviruses like encephalitis virus and West Nile virus, along with protozoa like <i>Plasmodium relictum</i> that cause bird malaria. With the increasing number of mosquito species, there is an increased need for additional species identification. More attention is being directed to the immature stage, specifically the pupae, due to their valuable characteristics for species identification. <i>Cx</i>. <i>quinquefaciatus</i>, one of the nine Culicine species found in Saudi Arabia, lacks a complete description of its pupal stage and only has partial descriptions of other developmental stages, making identification challenging. This study aimed to investigate the morphological characteristics of <i>Cx</i>. <i>quinquefaciatus</i> pupae, focusing on the cephalothorax, trumpet, abdomen, paddle, and genital lobes, as well as the distribution and arrangement of setal branching and chaetotaxy. The pupae were collected from Qaryat Al Ulya Governorate, Eastern region, KSA, and maintained in a Biology Laboratory at the University of Hafr Al Batin from September to October 2022. The findings of this study have provided the essential characteristics regarding the distinguishing features of <i>Cx</i>. <i>quinquefaciatus</i> pupae to distinguish it from other closely related species. This identification could assist field entomologists in enhancing their efficiency by classifying mosquitoes during virus outbreaks.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"478-488"},"PeriodicalIF":2.0,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-Space Disinfectants With Sodium Hypochlorite and Methylene Blue Loaded in Silver and Quartz Nanoparticles Activated by Photodynamic Therapy. A SEM Analysis","authors":"Fahad Alkhudhairy,&nbsp;Yasser F. AlFawaz","doi":"10.1002/jemt.24712","DOIUrl":"10.1002/jemt.24712","url":null,"abstract":"<div>\u0000 \u0000 <p>Post-space disinfectants methylene blue loaded with silver (Ag) and Quartz nanoparticles (NPs) and MB alone activated by photodynamic therapy (PDT) on the survival of <i>Enterococcus faecalis</i>, smear layer (SL) removal efficacy, and extrusion bond strength (EBS) of fiber posts to canal dentin. Hundred mandibular premolars underwent root canal treatment using the rotary ProTaper system. The canals were obturated and post-space was prepared up to a length of 8 mm, maintaining a 5 mm seal. To assess antibacterial efficacy <i>E. faecalis</i> were inoculated in the canal (<i>n</i> = 20). The samples were then randomly allocated into four groups according to the canal disinfectant used to sterilize the canals. Group 1: 2.5% NaOCl+17% EDTA, Group 2: MB-PDT, Group 3: MB@QP-PDT, and Group 4: MB@AgNP-PDT (<i>n</i> = 25). SEM analysis was conducted on five samples from each disinfectant group to assess the removal of the SL. Survival rates were calculated (<i>n</i> = 5 from each group). Glass fiber post (GFP) was cemented to the root dentin of the remaining samples followed by artificial aging. Sectioning of the specimens was performed in all three-thirds of the canals. PBS was assessed followed by failure evaluation. ANOVA and Tukey post hoc test were used to compare the <i>E. faecalis</i> survival rate and PBS of fiber post among different investigated groups. Group 4 (MB@AgNP-PDT) treated canals exhibited the minimum survival rate (0.30 ± 0.04 CFU/mL) of <i>E. faecalis</i> and maximum PBS. However, the highest survival rate and minimum bond strength of GFP were observed in Group 1 (NaOCl+17% EDTA) and Group 2 (MB-PDT) treated teeth respectively. The highest SL removal was recorded in the coronal section of the samples of Group-4 disinfected with MB@AgNP-PDT. The lowest removal of SL was recorded in Group 2 samples sterilized with MB-PDT at apical one-third. Silver nanoparticles when utilized as nano-carriers to enhance the efficiency of MB activated by PDT, have been shown to exhibit the highest antimicrobial potency, improved capacity for SL removal and improved PBS.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"468-477"},"PeriodicalIF":2.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light Microscopic and Scanning Electron Microscopic Techniques to Characterize Nutlets of Some Indian Cyperoideae (Cyperaceae) and Their Taxonomic Significance","authors":"Subrata Majumder,&nbsp;Pooja Rajak,&nbsp;Vikram Kr. Das,&nbsp;Usha Das,&nbsp;Asima Mandal,&nbsp;Asok Ghosh","doi":"10.1002/jemt.24707","DOIUrl":"10.1002/jemt.24707","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 &lt;p&gt;In the present investigation, nutlet morphological and micro-morphological characters were analyzed using Light Microscope (LM) and Scanning Electron Microscope (SEM) in 38 taxa under 13 genera from the sub-family Cyperoideae of Cyperaceae to find out whether these characters are taxonomically important or not. Nutlet morphology and surface characters of the representative taxa from all the possible tribes under the sub-family Cyperoideae namely, Fuireneae, Cypereae, Cariceae, Abildgaardieae, Eleocharideae, Pseudoschoeneae, Schoenoplecteae, and Sclerieae were evaluated for their taxonomic significance. Cluster analysis was employed considering nutlet morphological characters to determine the overall similarity among the taxa based on 153 character states. Except in few specified cases, grouping of the taxa in the clusters is in accordance to the taxonomic treatments made by recent Cyperologists. Nutlets in the tribe Abildgaardieae showed maximum level of variability in size, shape, and surface ornamentation at the level of higher taxon, but showed specificity at the species level. Tuberculate, striate-reticulate, and transversely wavy ridged surface ornamentations were found in different species of &lt;i&gt;Fimbristylis&lt;/i&gt;. Previously described cryptic variation, and effect of different ploidy level were not reflected in nutlet surface morphology and micromorphology in &lt;i&gt;Fimbristylis dichotoma&lt;/i&gt; and &lt;i&gt;F. ovata&lt;/i&gt; complexes which was previously made the taxa taxonomically very difficult. Serrulate anticlinal wall in &lt;i&gt;F. bisumbellata&lt;/i&gt; was the most unique in &lt;i&gt;Fimbristylis&lt;/i&gt;. Species under the megadiverse genus &lt;i&gt;Carex&lt;/i&gt; representing the tribe Cariceae showed very unique type of surface ornamentation. Surface walls of all the studied species of &lt;i&gt;Carex&lt;/i&gt; were characterized by polygonal epidermal cells with single conical silica body (2–3 per cell in &lt;i&gt;C. speciosa&lt;/i&gt;) of variable length and sizes. Most interestingly, in &lt;i&gt;C. nubigena&lt;/i&gt;, presence of the central silica body and peripheral satellites was not consistent. Based on the presence and absence, two different variants under the species were identified. In &lt;i&gt;C. nubigena&lt;/i&gt;, when silica body was present, epidermal cells were characterized by central conical silica body surrounded by variable number of satellites. The present investigation first time reports this novel nutlet surface character in &lt;i&gt;C. nubigena&lt;/i&gt;. Among the studied characters, length and height of conical, height of apex, and width of apex are variable among &lt;i&gt;Carex&lt;/i&gt; species. On the other hand, exclusion of the genera &lt;i&gt;Schoenoplectiella&lt;/i&gt; and &lt;i&gt;Schoenoplectus&lt;/i&gt; from tribe Scirpeae &lt;i&gt;s.l.&lt;/i&gt; and placement under the tribes Pseudoschoeneae and Schoenoplecteae (respectively) was also supported by the present investigation. The present study also confirms that nutlet morphological and micro-morphological characters are useful in identification and arrangement of different ","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"428-467"},"PeriodicalIF":2.0,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of Programmed Cell Death and Autophagy on Schizogenous Secretory Canal Formation in Angelica dahurica var. formosana Root","authors":"Jiahui Yang,&nbsp;Boyuan Li,&nbsp;Yue Qin,&nbsp;Jing Gao,&nbsp;Xiaofei Zhang,&nbsp;Xinjie Yang,&nbsp;Gang Zhang,&nbsp;Yonggang Yan,&nbsp;Ying Chen","doi":"10.1002/jemt.24711","DOIUrl":"10.1002/jemt.24711","url":null,"abstract":"<div>\u0000 \u0000 <p>Schizogenous secretory canal is an important functional structure of <i>Angelica dahurica</i> var. <i>formosana</i>, which is the main part of the accumulation of essential oil, coumarin and other components. The developmental process of secretory tissue and the accumulation of components are closely related. Meanwhile, programmed cell death (PCD) plays an important role in the development of plant secretory tissues, which is usually associated with autophagy. However, there are fewer studies involving PCD and autophagy with the development of schizogenous secretory canal. This study aims to provide new data on the development of schizogenous secretory canal in <i>A. dahurica</i> var. <i>formosana</i>. Light and transmission electron microscopy were used to reveal the cytological characteristics of secretory canal in <i>A. dahurica</i> var. <i>formosana</i> roots at different developmental stages. PCD and autophagy signals during the developmental process were detected using techniques such as terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) detection, propidium iodide (PI) staining, and immunofluorescent labeling. The results showed that the walls of secretory cells were intact during the development of schizogenous secretory canal in <i>A. dahurica</i> var. <i>formosana</i> roots. Mature secretory cells showed high vacuolization and accumulated a large number of essential oils. Meanwhile, we also observed significant ultrastructural features of PCD and autophagy during the developmental process. The signal detection results indicated that PCD and autophagy were jointly involved in the development of schizogenous secretory canal in <i>A. dahurica</i> var. <i>formosana</i> roots, and caspase-3-like protease may act as an upstream signal and participate in PCD.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"416-427"},"PeriodicalIF":2.0,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142470110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic Characterization of the Infectious Process, ROS Production, and Fungi Cellular Death of Alternaria alternata on Tangerine Resistant to QoIs","authors":"Thiago Oliveira Condé,&nbsp;Adriano Francis Dorigan,&nbsp;Silvino Intra Moreira,&nbsp;Patricia Ricardino da Silveira,&nbsp;Eduardo Alves","doi":"10.1002/jemt.24710","DOIUrl":"10.1002/jemt.24710","url":null,"abstract":"<div>\u0000 \u0000 <p>Quinone outside inhibitor (QoI) fungicide resistance in <i>Alternaria alternata</i> populations was reported in Brazil for the first time in 2019, in São Paulo orchards, and the mutation G143A in cytochrome b (<i>cytb</i>) was found in resistant isolates. Our study investigated the infectious process, production of reactive oxygen species (ROS), and fungal cell death in resistant (QoI-R) and sensitive (QoI-S) <i>A</i>. <i>alternata</i> pathotype tangerine (Aapt) isolates. Morphological characterization of Aapt isolates was performed using confocal laser scanning microscopy (CLSM). Alternaria brown spot (ABS) symptoms were produced by Aapt isolates on tangelo cv. BRS Piemonte. Germination of QoI-R conidia and production of germ tubes on tangelo leaflets treated with 100 μg mL⁻¹ of pyraclostrobin 18 h after inoculation (hai) was observed using scanning electron microscopy (SEM). At the same time, QoI-S conidial germination was inhibited on tangelo leaflets treated with pyraclostrobin. ROS production and cell death in Aapt isolates at high fungicide concentrations were observed using CLSM. QoI-S conidia exhibited high ROS production, indicating high oxidative stress. When dyed with propidium iodate (PI), QoI-S conidia emitted red fluorescence, showing cell death and confirming their sensitive phenotype. In contrast, QoI-R conidia neither produced ROS nor exhibited red fluorescence, indicating no cell death and confirming their resistant phenotype. Therefore, our findings evidence that microscopic techniques may help characterize events during fungi–plant interactions, ROS production, cell death, and Aapt phenotypes resistant and sensitive to QoIs using fluorometric protocols.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"407-415"},"PeriodicalIF":2.0,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142400665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macroscopic and microscopic investigations of determining elasto-mechanical properties of limequat fruit","authors":"Sanaz Vatani,&nbsp;Mohammad Hossein Abbaspour-Fard,&nbsp;Rasool Khodabakhshian","doi":"10.1002/jemt.24699","DOIUrl":"10.1002/jemt.24699","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>Given the paramount importance of agricultural products in global health and food security, and the increasing consumer demand, understanding the mechanical behavior of these materials under various conditions is necessary yet challenging. Due to their heterogeneous and non-uniform nature, determining their mechanical behavior is complex. This study employs atomic force microscopy (AFM) to determine the modulus of elasticity of limequat fruit at the microscopic scale and compares it with macroscopic methods. The analyses revealed a statistically significant difference (at the 1% level) in the mechanical behavior determined at the macroscopic scale. The highest modulus of elasticity, 0.752 MPa, was observed using Hertz's theory under complete placement between two parallel planes. The lowest, 0.059 MPa, was noted when a spherical probe compressed a rectangular sample. The average modulus of elasticity of the limequat peel was 2.007 MPa. At the microscopic scale, the modulus of elasticity of the fruit tissue ranged from 0.370 to 0.365 MPa, and for the peel, it was 0.246 MPa.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Research Highlights</h3>\u0000 \u0000 <div>\u0000 \u0000 <ol>\u0000 \u0000 <li>Working principles of this innovative technique were elaborated.</li>\u0000 \u0000 <li>The AFM technique used provide elasto-mechanical properties determination of cell walls of single living cells extracted from biological materials on the nanoscale.</li>\u0000 \u0000 <li>By combining AFM topographical image and nano-indentation of living fruit cells it will be possible to investigate cells' elasto-mechanical properties.</li>\u0000 \u0000 <li>Atomic force microscopy holds great potential for monitoring fruit mechanical properties of biological materials.</li>\u0000 </ol>\u0000 </div>\u0000 </section>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 2","pages":"396-406"},"PeriodicalIF":2.0,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}