Microscopy Research and Technique最新文献_第9页

Self-Assembly Behavior of Zein on Two Different Substrates Visualized by Atomic Force Microscopy. 用原子力显微镜观察 Zein 在两种不同基底上的自组装行为。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-01-02 DOI: 10.1002/jemt.24794

Jing Hu, Mengnan Liu, Litong Dong, Jie Luo, Liang Cao, Mingyan Gao, Zuobin Wang

{"title":"Self-Assembly Behavior of Zein on Two Different Substrates Visualized by Atomic Force Microscopy.","authors":"Jing Hu, Mengnan Liu, Litong Dong, Jie Luo, Liang Cao, Mingyan Gao, Zuobin Wang","doi":"10.1002/jemt.24794","DOIUrl":"https://doi.org/10.1002/jemt.24794","url":null,"abstract":"When protein molecules come into contact with different types of substrate materials, the surface properties of the substrate will have a significant effect on their self-assembly behavior. The purpose of this study was to investigate the self-assembly behavior of zein molecules on the two different substrates. Herein, the microstructure of zein molecules on the surface of two typical substrates, mica and glass, were characterized in detail by atomic force microscopy. It was found that zein molecules self-assemble to form spherical structures with uniform size and close arrangement on mica substrates. Compared with mica, the rough glass surface possesses a larger water contact angle, which leads to weaker interaction between zein molecules and its surface, thus enhancing the interaction between zein molecules. Therefore, the zein molecules on the glass substrate exhibit a distinct hierarchical arrangement of one large globule surrounded by many smaller ones. This work provides valuable information for further study of the self-assembly behavior of zein molecules on the substrate surface.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waveguide Evanescent Field Fluorescence Microscopy Images of Osteoblast Cells: The Effect of Trypsin and Image Processing Using TrackMate. 成骨细胞的波导倏逝场荧光显微镜图像：胰蛋白酶的影响和使用TrackMate进行图像处理。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-01-02 DOI: 10.1002/jemt.24766

Abdollah Hassanzadeh, Seyed Navid Elyasi, Siyamand Salih, Sarkew Salah Abdulkareem, Salah Raza Saeed

{"title":"Waveguide Evanescent Field Fluorescence Microscopy Images of Osteoblast Cells: The Effect of Trypsin and Image Processing Using TrackMate.","authors":"Abdollah Hassanzadeh, Seyed Navid Elyasi, Siyamand Salih, Sarkew Salah Abdulkareem, Salah Raza Saeed","doi":"10.1002/jemt.24766","DOIUrl":"https://doi.org/10.1002/jemt.24766","url":null,"abstract":"Waveguide evanescent field fluorescence microscopy (WEFF) is an evanescent-based microscopy that utilizes a confined thin film of light, around 100 nm, to image the plasma membrane of cells attached to a waveguide. Low photobleaching and low background besides its high axial resolution allows time-lapse imaging to investigate changes in cell morphology in the presence or absence of chemical agents. Both large field of view (FOV) and uniform illumination are very important while imaging cell-substrate contacts with an evanescent field. In the current study, we demonstrate that the WEFF microscope is capable of large FOVs with a uniform illumination source and imaging over a very long time period with a simple and inexpensive experimental setup. The interaction of the trypsin with plasma membranes of live osteoblast cells is investigated. To analyze cell images (250 images), instead of relying on manual tracking, which is time-consuming and can introduce numerous errors, we performed image processing using TrackMate to investigate the dynamic response of cells upon exposure to trypsin. This helps to save time and increase the accuracy of the analysis. The powerful tracking and analysis capabilities of the TrackMate plugin in ImageJ are used to automatically detect the cells border and trace each cluster of cells. The reduction in cell area is accompanied by a notable increase in mean intensity, reflecting changes in the intracellular environment. However, the background did not change during the experiment, which proves that the fluorescence material remains attached to the cell membrane and does not leak into the cell medium.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological, Anatomical, and Histochemical Study of Cordia diffusa K.C. Jacob-A Steno Endemic Plant. 台湾特有植物白花草的形态、解剖及组织化学研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-31 DOI: 10.1002/jemt.24791

M Haritha, D Leena Lavanya, T Muthukumar

{"title":"Morphological, Anatomical, and Histochemical Study of Cordia diffusa K.C. Jacob-A Steno Endemic Plant.","authors":"M Haritha, D Leena Lavanya, T Muthukumar","doi":"10.1002/jemt.24791","DOIUrl":"https://doi.org/10.1002/jemt.24791","url":null,"abstract":"Cordia diffusa K.C. Jacob, known as Sirunaruvili, belonging to the family Boraginaceae, is a rare endemic species. The study aimed to document the morpho-anatomical and histochemical characteristics of C. diffusa to facilitate its identification, as it is a highly threatened steno-endemic plant. The plant is an evergreen, woody shrub with brownish-gray, lenticellate stems and oblong-oblanceolate with pubescent leaves. Flowers are yellowish-white, tubular, fragrant, and arranged in umbellate cyme. Fruits and drupe, turning from green to orange when ripe, with viscid edible pulp. Flowering and fruiting occur year-round. The anatomical results showed that the adaxial surface of leaves had more stomata than the abaxial leaf surface. The pubescent plant surface was due to unicellular trichomes in the leaf's epidermal layer, and some of the epidermal cells had cystoliths. Petioles and stems had ridges, unicellular trichomes, and collateral vascular bundles with prominent water storage cells. Root exhibited radial vascular arrangements, cortical region, endodermis, and exarch xylem, with secondary growth forming periderm layers. Sclerenchymatous fibers and lignin accumulation were observed in the stem and roots. The histochemical study showed the presence of carbohydrates, proteins, lipids, acidic and neutral lipids, phenolic compounds, lignified cell walls, and alkaloids in various regions of the plant cell. The micro-morphological properties of the plant exhibited several unique aspects for accurate taxonomic identification and species delimitation, and this work offers many valuable details to plant taxonomists for future research.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extrachromosomal DNA in Breast Cancer Cell Lines: Detection and Characterization 乳腺癌细胞系的染色体外DNA：检测和表征。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-29 DOI: 10.1002/jemt.24780

Shadira Anindieta Irdianto, Fadhillah Fadhillah, Retno Lestari, Fadilah Fadilah, Anom Bowolaksono, Astari Dwiranti

{"title":"Extrachromosomal DNA in Breast Cancer Cell Lines: Detection and Characterization","authors":"Shadira Anindieta Irdianto, Fadhillah Fadhillah, Retno Lestari, Fadilah Fadilah, Anom Bowolaksono, Astari Dwiranti","doi":"10.1002/jemt.24780","DOIUrl":"10.1002/jemt.24780","url":null,"abstract":"<div>\u0000 \u0000 This study delves into the intriguing world of extrachromosomal DNA (ecDNA) in breast cancer, uncovering its pivotal role in cancer's aggressiveness and genetic variability. ecDNA, a form of circular DNA found outside chromosomes, is known to play a significant role in cancer progression by increasing oncogene expression. Focusing on two contrasting cell lines, MDA-MB-231 (triple-negative) and MCF-7 (Luminal-A), we utilized advanced microscopy and fluorescence techniques to detect and characterize ecDNA. Our findings reveal a stark difference: MDA-MB-231 cells, known for their high metastatic potential, exhibit a striking abundance of ecDNA, manifested as double minutes and single form with intense fluorescence signals. In contrast, the less aggressive MCF-7 cells harbor significantly fewer ecDNA. This disparity highlights the potential of ecDNA as a key player in cancer progression and a promising target for novel therapies. This research sheds light on the unseen genetic forces driving breast cancer and opens the door to new strategies in cancer treatment. Further research is necessary to understand the mechanisms of ecDNA formation and its role in different breast cancer subtypes.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1239-1247"},"PeriodicalIF":2.0,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of the Eyelids of the Black-Winged Kite, Elanus caeruleus in the Immune Protection of the Eye 黑翼鸢（Elanus caeruleus）眼睑在眼睛免疫保护中的作用。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-29 DOI: 10.1002/jemt.24754

Nahed Ahmed Shawki, Abdelraheim H. Attaai, Amany Mohamed Abdel-Mageed, Fatma Abdel-Regal Mahmoud

{"title":"The Role of the Eyelids of the Black-Winged Kite, Elanus caeruleus in the Immune Protection of the Eye","authors":"Nahed Ahmed Shawki, Abdelraheim H. Attaai, Amany Mohamed Abdel-Mageed, Fatma Abdel-Regal Mahmoud","doi":"10.1002/jemt.24754","DOIUrl":"10.1002/jemt.24754","url":null,"abstract":"<div>\u0000 \u0000 This study aimed to describe the morphological features and microstructure of the upper, lower, and third eyelids of the black-winged kite, Elanus caeruleus, and to characterize the organized lymphoid follicles and lymphocytes in the eyelid mucosa. Additionally, it aimed to illustrate the importance of the eye adnexa in the eye's immune protection. The upper, lower, and third eyelids display varying morphological differences that seem to be closely linked to the birds' way of life, indicating adjustments to their environment and eating behaviors. The black-winged kite has large, forward-facing eyes positioned under a bony shelf (lacrimal process) that shades them. Both eyelids have thick, pigmented edges and bear two rows of long, finely modified filoplume feathers, which increase at the anterior canthus. Melanocytes appear in the stratum basale of upper and lower eyelids, and Langerhans cells were observed. Aggregations of lymphatic cells were present under the conjunctival epithelium within the stroma of the lower eyelid (in the orbital zone near the tarsal plate), while they were absent in the upper eyelid and nictitating membrane. The density of goblet cells on the conjunctival surface of the upper eyelid and the nictitating membrane is higher than that of the lower eyelid. The structure of the skin and conjunctiva in both the upper and lower eyelids contains numerous defensive immune cells that maintain ocular safety and hydration. Additionally, the lower eyelid's CALT constitutes most of the CALT tissue in E. caeruleus and is recognized as part of the mucosal immune system.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1207-1222"},"PeriodicalIF":2.0,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A New Marine Epizoic Diatom, Ceratanaulus alaris sp. nov. (Bacillariophyceae), From Guangxi Zhuang Autonomous Region, the Southwest Coast of China 中国西南沿海广西壮族自治区一种新的海洋动物硅藻，ceratanulus alaris sp. 11（硅藻科）。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-29 DOI: 10.1002/jemt.24785

Longjia Huang, Lin Sun, Junrong Liang, Yahui Gao, Changping Chen

{"title":"A New Marine Epizoic Diatom, Ceratanaulus alaris sp. nov. (Bacillariophyceae), From Guangxi Zhuang Autonomous Region, the Southwest Coast of China","authors":"Longjia Huang, Lin Sun, Junrong Liang, Yahui Gao, Changping Chen","doi":"10.1002/jemt.24785","DOIUrl":"10.1002/jemt.24785","url":null,"abstract":"<div>\u0000 \u0000 Diatoms (Bacillariophyceae) are diverse and abundant microalgae, and many of them are found in marine intertidal environments. Here, we present a new marine epizoic diatom isolated from Euspira gilva Philippi in intertidal area of Beihai city, Guangxi Zhuang Autonomous Region, named Ceratanaulus alaris L.J. Huang, Y.H. Gao & C.P. Chen sp. nov. Using light microscope (LM) and scanning electron microscope (SEM), we observed and described the morphology of C. alaris in detail. The valve center of C. alaris features a rimoportula with an external tube. The most notable characters in C. alaris are wing-like structures on both sides of the central tube. These structures extend from the central tube to the horn-like protrusions at the valve apices, with fissures evenly distributed on the wing-like structures. In this study, we compared the morphological structures of the new species with other species in the genus Ceratanaulus Górecka, Witkowski, Dabek & Ashworth, enhancing the understanding of the ultrastructure of C. alaris and facilitating the differentiation of this species from closely related taxa. The genus, Ceratanaulus, is newly recorded in China, thereby expanding the known geographical distribution of the genus.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1255-1260"},"PeriodicalIF":2.0,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaf Micromorphological Characteristics of Korean Rush and Their Taxonomic Implications Based on Microscopic Analysis

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-29 DOI: 10.1002/jemt.24772

Young-Min Choi, Bokyung Choi, Changyoung Lee, Jin-Hyub Paik, Tae-Soo Jang

{"title":"Leaf Micromorphological Characteristics of Korean Rush and Their Taxonomic Implications Based on Microscopic Analysis","authors":"Young-Min Choi, Bokyung Choi, Changyoung Lee, Jin-Hyub Paik, Tae-Soo Jang","doi":"10.1002/jemt.24772","DOIUrl":"https://doi.org/10.1002/jemt.24772","url":null,"abstract":"<div>\u0000 \u0000 The genus Juncus L., comprising approximately 310 species, is a perennial herbaceous plant with the highest species diversity among Juncaceae. Although external leaf morphological and anatomical characteristics were used for taxonomic delimitation of Korean Juncus taxa, the micromorphological structure of Juncus is not comprehensively described. Our main objective was to determine whether leaf micromorphological comparisons among the studied Korean Juncus taxa could provide taxonomically informative characteristics. Korean rushes possessed both unifacial and bifacial leaves, although the patterns of cell outlines on the abaxial surfaces of cataphyll and cauline leaves and stems were similar. Leaf and stem epidermal cells were irregularly arranged and exhibited rectangular, rhomboidal, and polygonal shapes. Striations on epidermal cells are common in almost all species of the genus Juncus; however, scaly epicuticular wax is found only in J. setchuensis. The stomatal complexes of the studied Korean Juncus taxa were uniformly paracytic (stomata surrounded by two subsidiary cells), regardless of their occurrence in cataphyll and cauline leaves or on the stem surface. Guard cell length could be important taxonomic characters in Korean Juncus taxa, in accordance with traditional taxonomy, which distinguishes the two subgenera based on morphological characters. Differences in guard cell length and stomatal density were also influenced by external environmental factors but were not clearly correlated with variations in ploidy levels.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1223-1238"},"PeriodicalIF":2.0,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of an Innovative Embedding and Cooling Component in the Critical Steps of Fragmented Tissue Pathological Diagnosis 一种创新的嵌入和冷却组件在碎片组织病理诊断关键步骤中的应用。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-29 DOI: 10.1002/jemt.24781

Xin Huang, Yuhuan Wang, Meihua Ye, Qingquan Fang

{"title":"Application of an Innovative Embedding and Cooling Component in the Critical Steps of Fragmented Tissue Pathological Diagnosis","authors":"Xin Huang, Yuhuan Wang, Meihua Ye, Qingquan Fang","doi":"10.1002/jemt.24781","DOIUrl":"10.1002/jemt.24781","url":null,"abstract":"<div>\u0000 \u0000 This study aimed to explore the application value of an innovative embedding and cooling component in the critical steps of fragmented tissue pathological diagnosis. Eighty small and fragmented tissue samples were collected and randomly divided into two groups. The embedding qualification rate and embedding efficiency were compared between the two groups. Seventy cases of fragmented tissue were collected, with three pieces of fragmented tissue taken from each case. The samples were randomly divided into three groups for sectioning. The wrinkle-free scores and sectioning efficiency were compared between different cooling methods. The embedding qualification rate was 90.0% in the embedding tweezer group, which was lower than 98.8% in the innovative component group (χ2 = 4.24, p < 0.05). The average embedding time per sample was 25.08 ± 4.61 s in the embedding tweezer group, which was significantly longer than the 16.69 ± 3.54 s in the innovative component group (t = 12.91, p < 0.001). The wrinkle-free section scores were 8.24 ± 0.69 in the small ice block cooling group, 8.11 ± 0.74 in the freezing platform cooling group, and 8.85 ± 0.65 in the innovative component cooling group, with the innovative component cooling group scoring significantly higher than the other two groups (q = 7.40, 8.98, p < 0.01). The innovative component cooling group had a shorter average sectioning time than the other two groups (q = 3.12, 26.75, p < 0.05, 0.01). The use of the innovative embedding and cooling component optimizes the embedding and sectioning steps in the pathological diagnosis of fragmented tissue. This method is worthy of promotion and application.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1248-1254"},"PeriodicalIF":2.0,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopic Observation and Physicochemical Analysis Reveal the Microstructure and Chemical Composition of Abelmoschus manihot Flower and Its Potential Value in Anti-Caries Applications 显微观察和理化分析揭示了马尼芒花的显微结构、化学成分及其潜在的防龋应用价值。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-26 DOI: 10.1002/jemt.24782

Zenghong Sun, Linyang Wang, Pin Chen, Shuge Tian, Yan Chen

{"title":"Microscopic Observation and Physicochemical Analysis Reveal the Microstructure and Chemical Composition of Abelmoschus manihot Flower and Its Potential Value in Anti-Caries Applications","authors":"Zenghong Sun, Linyang Wang, Pin Chen, Shuge Tian, Yan Chen","doi":"10.1002/jemt.24782","DOIUrl":"10.1002/jemt.24782","url":null,"abstract":"<div>\u0000 \u0000 Abelmoschus manihot flower (AMF), commonly cultivated in Southern China, is homology of medicine and food. In this study, microscopic observation revealed the microstructure of AMF, including upper epidermal cells and nonglandular hairs that play roles in defense and water management. Physicochemical analyses indicated that AMF powder exhibits weak acidity and low moisture content, suggesting its stability. Further fluorescence and spectroscopic analyses identified a diverse array of chemical constituents in AMF, including various flavonoids. Using ultra high performance liquid chromatography_quadrupole exactive hybrid mass spectrometer (UHPLC-Q Exactive HFX) technology, a total of 407 compounds were identified in AMF, 79 of which were found to have a relative content exceeding 0.1%. A network pharmacological analysis identified 31 potential active components and delineated 10 core targets associated with dental caries. Molecular docking studies demonstrated that the selected active components exhibited strong binding affinities with the aforementioned core targets, particularly flavonoids such as quercetin and morin. In conclusion, this study provides a comprehensive analysis of AMF's microstructure and chemical composition, establishing a scientific foundation for its applications in the pharmaceutical and food industries, as well as in oral health for caries prevention, while also exploring its potential mechanisms of action.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1181-1206"},"PeriodicalIF":2.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anatomical and Physicochemical Attributes of Endemic Medicinal Plant Species Vincetoxicum capparidifolium (Wight & Arn.) Kuntze, Apocynaceae 地方性药用植物capparidifolium （Vincetoxicum capparidifolium, Wight & Arn.）的解剖和理化性质Kuntze,夹竹桃科。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-25 DOI: 10.1002/jemt.24783

Athira Prameela, Thenmozhi Krishnasamy, Malavika Jayasree, Muthukumar Thangavelu

{"title":"Anatomical and Physicochemical Attributes of Endemic Medicinal Plant Species Vincetoxicum capparidifolium (Wight & Arn.) Kuntze, Apocynaceae","authors":"Athira Prameela, Thenmozhi Krishnasamy, Malavika Jayasree, Muthukumar Thangavelu","doi":"10.1002/jemt.24783","DOIUrl":"10.1002/jemt.24783","url":null,"abstract":"<div>\u0000 \u0000 Vincetoxicum capparidifolium (Wight & Arn.) Kuntze [=Tylophora capparidifolia (Wight & Arn.) Kuntze], belonging to the family Apocynaceae, is a medicinal plant species endemic to the southern Western Ghats, Tamil Nadu, India. The current study sought to investigate the macroscopic, organoleptic, microscopic, physicochemical, and proximate compositional aspects of the fresh and powdered leaf and stem portions of V. capparidifolium. The anatomical peculiarities of the leaf parts reveal a hypostomatic nature with paracytic stomata, the epidermis being made up of thin-walled cells covered with a thick cuticle (5.9 μm), and the hypodermis comprising angular collenchyma cells. The petiole is oval/rounded-ellipse with abundant nonglandular multicellular trichomes ascending from the epidermis. The hypodermis is composed of collenchymatous cells containing many calcium oxalate crystals and silica bodies. Bicollateral vascular bundles with internal and external phloem characterize the leaf, stem, and petiole parts. The stem is covered by a thin cuticle, chlorenchymatous hypodermis, and large gelatinous fiber bundles (124.29 × 81.71 μm). Secondary growth in the stem is characterized by the development of periderm and lignified vascular tissues. Bicollateral vascular bundles are overlaid by irregular sclerenchyma patches (7.07 × 5.36 μm), a parenchymatous cortex, and pith composed of thin-walled parenchyma cells. Scanning electron microscopic study of powdered plant parts disclosed the presence of fiber in the stem and a trace outline of leaf epidermal cells. X-ray diffraction analysis specified indefinite crystallinity in the plant powder (57.038–69.500 nm). Thorough examination of pH, ash content, and percentage of crude lipid confirms that V. capparidifolium exhibits sufficient quality and purity.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 4","pages":"1167-1180"},"PeriodicalIF":2.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142886039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0