Microscopy Research and Technique最新文献_第9页

Combining Correlative Cryogenic Fluorescence and Electron Microscopy and Correlative Cryogenic Super-Resolution Fluorescence and X-Ray Tomography—Novel Complementary 3D Cryo-Microscopy Across Scales to Reveal Nanoparticle Internalization Into Cancer Cells 结合相关低温荧光和电子显微镜，相关低温超分辨率荧光和x射线断层扫描-新型互补三维低温显微镜跨尺度揭示纳米颗粒内化到癌细胞。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-09-01 DOI: 10.1002/jemt.70071

Pavitra Sokke Rudraiah, Louisa Herbsleb, Michaela Salakova, Henriette Gröger, Anna Maria Steyer, Frauke Alves, Claus Feldmann, Andreas Walter

{"title":"Combining Correlative Cryogenic Fluorescence and Electron Microscopy and Correlative Cryogenic Super-Resolution Fluorescence and X-Ray Tomography—Novel Complementary 3D Cryo-Microscopy Across Scales to Reveal Nanoparticle Internalization Into Cancer Cells","authors":"Pavitra Sokke Rudraiah, Louisa Herbsleb, Michaela Salakova, Henriette Gröger, Anna Maria Steyer, Frauke Alves, Claus Feldmann, Andreas Walter","doi":"10.1002/jemt.70071","DOIUrl":"10.1002/jemt.70071","url":null,"abstract":"<p>Understanding the intracellular fate of nanoparticles (NPs) is essential for advancing nanomedicine, particularly in targeted drug delivery for cancer therapy. Here, we present a complementary cryogenic microscopy workflow across scales to investigate the uptake and subcellular localization of zirconyl-containing inorganic–organic hybrid nanoparticles (IOH-NPs) in murine breast cancer cells. Our approach integrates cryogenic fluorescence microscopy (cryo-FM), cryo-focused ion beam scanning electron microscopy (cryo-FIBSEM), and cryo-soft X-ray tomography (cryo-SXT), enabling molecular specificity, high-resolution imaging, and volumetric ultrastructural analysis in near-native cellular states. We demonstrate that the cryogenic workflow provides enough contrast and resolution across all modalities for quantifying the IOH-NP uptake: NPs are internalized within 2 h of incubation and progressively accumulate in endolysosomes over time, as confirmed by fluorescence labeling and SXT. Quantitative analysis reveals a marked increase in endolysosomal accumulation of IOH-NPs from 2 to 24 h. Our findings help to establish multimodal cryogenic microscopy as a powerful tool for nanoscale imaging and quantitative analysis of NP uptake within close-to-native cells, offering new insights into NP trafficking and cellular responses relevant to nanomedicine development.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 2","pages":"260-272"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12803693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microscopic Analysis of Anatomical and Taxonomic Characteristics in Some Centaurea Species 一些半人马属植物解剖和分类特征的显微分析。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-31 DOI: 10.1002/jemt.70068

Şükran Öztürk, Öznur Ergen Akçin

{"title":"Microscopic Analysis of Anatomical and Taxonomic Characteristics in Some Centaurea Species","authors":"Şükran Öztürk, Öznur Ergen Akçin","doi":"10.1002/jemt.70068","DOIUrl":"10.1002/jemt.70068","url":null,"abstract":"<div>\u0000 \u0000 <p>This study was carried out using light and scanning electron microscopy to examine in detail the anatomical and micromorphological characteristics of roots, stems, and leaves of six <i>Centaurea</i> taxa (two of which are endemic) to determine the characters that are important for the taxa studied. For anatomical investigations, transverse and superficial sections were taken from root, stem, and leaf organs and examined by light microscopy. For micromorphological assessments, dried leaf surfaces were analyzed via scanning electron microscopy (SEM). Several statistical methods were used for data analysis. A total of 38 characters were considered to determine the anatomical differences among the taxa. Anatomical analyses indicated that variations in the structure of sclerenchyma, phloem, and cortex parenchyma in root; epidermis, chlorenchyma, sclerenchyma, and phloem in stem; and upper epidermis, upper, and lower stomatal characteristics in leaf, including stomatal and epidermal cell density, were statistically significant among the taxa. SEM investigations revealed the presence of four types of trichomes and extrafloral nectaries (only <i>C. carduiformis</i> DC. subsp. <i>carduiformis</i> var. <i>carduiformis</i>) on the leaf surfaces.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 2","pages":"243-259"},"PeriodicalIF":2.1,"publicationDate":"2025-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chromosome Image Classification Using Edge Fusion Attention Network 基于边缘融合注意网络的染色体图像分类。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-30 DOI: 10.1002/jemt.70070

V. Praveena, S. Anbumani, M. Nirmala, S. Karthik

{"title":"Chromosome Image Classification Using Edge Fusion Attention Network","authors":"V. Praveena, S. Anbumani, M. Nirmala, S. Karthik","doi":"10.1002/jemt.70070","DOIUrl":"10.1002/jemt.70070","url":null,"abstract":"<div>\u0000 \u0000 <p>Identification of chromosome pairs is important for karyotype generation and genetic disease prediction. Conventional methods tend to fail with structural variations of the chromosomes and imprecise boundaries. To solve these issues, we propose a deep learning architecture, Edge Fusion Attention Network (EFANet), for chromosome classification. It is a new architecture that combines the Adaptive Edge Preserve Fusion (AEPF) algorithm with the Feature Focused Attention Network (F<sup>2</sup>ANet). The AEPF algorithm clearly identifies chromosome boundaries and highlights the morphological differences. It improves feature representation by combining edge features with intensity, thereby ensuring accurate classification. The F<sup>2</sup>ANet block in EFANet improves classification using three main components: a feature extraction block, an attention block with both channel and spatial attention, and a classification block. Our proposed method ensures accurate chromosome classification, which is essential for diagnosing genetic disorders such as aneuploidies and translocations. Edge detection, a key feature of EFANet, enhances the identification of chromosome abnormalities by focusing more on unusual shapes than normal ones. Our proposed EFANet showed strong performance with 99.5% accuracy, 99.48% F1 score, 99.63% precision, and 99.45% recall. These results highlight its effectiveness in edge detection, which is important for improving automated chromosome analysis. This approach tremendously improves karyotyping by overcoming the limitations of the traditional approaches, resulting in more accurate and timely genetic disease diagnosis and eventually better patient outcomes.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 2","pages":"230-242"},"PeriodicalIF":2.1,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacognostic Characterization of Primula denticulata by Light Microscopy, Scanning Electron Microscopy, and Analytical Techniques 用光镜、扫描电镜及分析技术研究报春花的生药学特征。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-28 DOI: 10.1002/jemt.70067

Kinza Muhammad Din, Fiaz Alam, Mehak Sarfraz, Rukhba Rasheed

{"title":"Pharmacognostic Characterization of Primula denticulata by Light Microscopy, Scanning Electron Microscopy, and Analytical Techniques","authors":"Kinza Muhammad Din, Fiaz Alam, Mehak Sarfraz, Rukhba Rasheed","doi":"10.1002/jemt.70067","DOIUrl":"10.1002/jemt.70067","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 <i>Primula denticulata</i> (Primulaceae) has long been used in traditional medicine to treat a variety of diseases. Around the globe, it is used to cure different diseases such as bronchitis, enhanced bronchial output, making phlegm less thick, asthma, joint discomfort, fever, diarrhea, sleeplessness, urinary infections, wound healing, eye illnesses, and dysuria. The study's findings provide several pharmacognostic benchmarks that support the maintenance of \u0000 <i>P. denticulata</i>\u0000 's purity, safety, and effectiveness. Light and scanning electron microscopes (SEM and LM) were used to investigate several plant sections, and cross-sectional images yielded several useful botanical features. During the macroscopic evaluation procedure, the form, color, size, odor, and surface characteristics of the plant parts were evaluated. The pharmacognostic standardization parameters (ash values, loss on drying, swelling index, hemolytic index, and foaming index) were determined in accordance with the guidelines provided by the World Health Organization. When the heavy metal content was examined using the atomic absorption spectrophotometer (AAS), it was discovered that both heavy metals and trace elements were found within allowable bounds. Qualitative phytochemical tests were performed on plant extracts to determine their contents, including alkaloids, flavonoids, saponins, glycosides, tannins, carbohydrates, lipids, and protein. Preliminary phytochemical testing indicates the major classes of phytoconstituents that are found in the plant. The quantitative and qualitative microscopic characteristics help to set criteria for planting pharmacopeia. Several pharmacognostic parameters, alike size, shape, and structure of various plant constituents described in connection with physicochemical and phytochemical examination, could be assessed with significant advantage for future research.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 2","pages":"218-229"},"PeriodicalIF":2.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Uniform Illumination Correction for Quantitative Fluorescence Imaging 定量荧光成像的非均匀照明校正。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-27 DOI: 10.1002/jemt.70056

Yue Wang, Yongqiang Liu, Beini Sun, Yan Mao, Tian Pang, Zhengfei Zhuang, Tongsheng Chen, Min Hu

{"title":"Non-Uniform Illumination Correction for Quantitative Fluorescence Imaging","authors":"Yue Wang, Yongqiang Liu, Beini Sun, Yan Mao, Tian Pang, Zhengfei Zhuang, Tongsheng Chen, Min Hu","doi":"10.1002/jemt.70056","DOIUrl":"10.1002/jemt.70056","url":null,"abstract":"<div>\u0000 \u0000 <p>Illumination uniformity is essential for quantitative analysis in fluorescence microscopy. However, fewer images or sparse intensity information can compromise correction quality of retrospective methods, whereas traditional prospective methods necessitate recalibration whenever imaging conditions change. In this paper, we theoretically for the first time analyze the impact of non-uniform illumination on quantitative fluorescence resonance energy transfer (FRET), which is also demonstrated experimentally. We proposed an illumination correction method based on fluorescent microscope slides, termed Systematic Uniform Fluorescence Image Correction (SUFIC). And we evaluated SUFIC using the images of fluorescent microspheres, Argo-HM slide, and live cells expressing FRET construct. After SUFIC correction, the field uniformity of the “field of rings” image in Argo-HM slide increased from 27.45% to 65.30%. The signal-to-background ratios of the FRET three-channel images increased by 12.3%, 7.9%, and 20.9%. Compared to the CIDRE and BaSiC methods, which require 100 input images for prospective correction, SUFIC requires only three reference images at minimum. In summary, SUFIC is a highly efficient prospective illumination correction method for quantitative microscopic imaging.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 1","pages":"164-178"},"PeriodicalIF":2.1,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144961505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface Symphony: Orchestrating DPPC/DOPC Monolayer Behavior 表面交响乐：协调DPPC/DOPC单层行为。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-27 DOI: 10.1002/jemt.70066

Wisnu Arfian A. Sudjarwo, Jose L. Toca-Herrera

引用次数: 0

Improvements in Microstructure, Mechanical and Degradation Properties of Mg–Y–Zn–Zr Alloy via Extrusion for Implant Applications 挤压法改善Mg-Y-Zn-Zr合金的显微组织、力学性能和降解性能。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-21 DOI: 10.1002/jemt.70038

Luo Xiaoping, Liu Baosheng, Hou Hua

引用次数: 0

Artemisia lerchiana Based Green Synthesis of Silver Nanoparticles: TEM Microscopy, Physicochemical, and Spectroscopic Characterization 绿色合成银纳米粒子：TEM显微镜，理化和光谱表征。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-19 DOI: 10.1002/jemt.70062

Gunay Hasanova, Sabina Omarova, Rovshan Khalilov, Afat Mammadova, Muhammad Zafar, Salman Majeed, Mohamed Fawzy Ramadan, Muhammad Rizwan Khan, Aleena Gul

{"title":"Artemisia lerchiana Based Green Synthesis of Silver Nanoparticles: TEM Microscopy, Physicochemical, and Spectroscopic Characterization","authors":"Gunay Hasanova, Sabina Omarova, Rovshan Khalilov, Afat Mammadova, Muhammad Zafar, Salman Majeed, Mohamed Fawzy Ramadan, Muhammad Rizwan Khan, Aleena Gul","doi":"10.1002/jemt.70062","DOIUrl":"10.1002/jemt.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>Silver nanoparticles (AgNPs) have unique physicochemical characteristics that render them very appropriate for various biomedical applications. AgNPs were synthesized in a study by an environmentally friendly and cost-effective method where an aqueous extract of <i>Artemisia lerchiana</i> was used as a bioreducing and stabilizing agent. To characterize the synthesized nanoparticles, a conjunction of analytical methods was employed, such as UV–Visible spectrophotometry, Fourier Transform Infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and atomic force microscopy (AFM). The UV–Vis spectrum indicated the formation of nanoparticles with a typical surface plasmon resonance peak situated at 480 nm. TEM examination showed mostly spherical-shaped nanoparticles with a size of 4–19 nm. FTIR spectra showed the presence of functional biomolecules that reduced silver ions and gave AgNPs stability. This green synthesis method highlights the possibility of using plant-based nanoparticle synthesis in biomedical, environmental, and industrial fields.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 1","pages":"148-155"},"PeriodicalIF":2.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144874167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Review of Recent Developments in Green Synthesis of Silver Nanoparticles: Antioxidant and Antibacterial Applications 纳米银绿色合成的最新进展：抗氧化和抗菌应用。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-15 DOI: 10.1002/jemt.70060

Kandasamy Selvam, Arunagiri Ragu Prasath, Abdullah K. Alanazi

{"title":"A Review of Recent Developments in Green Synthesis of Silver Nanoparticles: Antioxidant and Antibacterial Applications","authors":"Kandasamy Selvam, Arunagiri Ragu Prasath, Abdullah K. Alanazi","doi":"10.1002/jemt.70060","DOIUrl":"10.1002/jemt.70060","url":null,"abstract":"<p>Green synthesis of silver nanoparticles (AgNPs) using biological sources has emerged as a sustainable and eco-friendly alternative to conventional chemical and physical methods. This review highlights recent advancements in the green synthesis of AgNPs employing plant extracts, bacteria, fungi, and algae, with particular emphasis on their antioxidant and antibacterial applications. Phytochemicals such as flavonoids, polyphenols, and terpenoids act as natural reducing and stabilizing agents, contributing to the formation of stable nanoparticles with enhanced bioactivity. The unique properties of AgNPs, including their large surface area and unique morphology, enable effective free radical scavenging and potent antibacterial activity, offering promising solutions to oxidative stress-related diseases and multidrug-resistant bacterial infections. This review critically compares green synthesis approaches with traditional methods, outlines their advantages and limitations, and explores the mechanisms underlying the antioxidant and antibacterial actions of AgNPs. The findings highlight the potential of green-synthesized AgNPs in biomedical and environmental applications.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 1","pages":"134-147"},"PeriodicalIF":2.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of RF Power on Structural and Corrosion Properties of Sputter-Deposited CrN Films 射频功率对溅射沉积CrN薄膜结构和腐蚀性能的影响。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-08-14 DOI: 10.1002/jemt.70059

Alireza Grayeli, Sahar Rezaee, Ştefan Ţălu

{"title":"Influence of RF Power on Structural and Corrosion Properties of Sputter-Deposited CrN Films","authors":"Alireza Grayeli, Sahar Rezaee, Ştefan Ţălu","doi":"10.1002/jemt.70059","DOIUrl":"10.1002/jemt.70059","url":null,"abstract":"<p>This study examines the deposition of chromium nitride (CrN) films on 304 stainless steel (304SS) substrates using radio-frequency (RF) magnetron sputtering, with RF power varied from 20 to 160 W in an argon atmosphere. The structural, topographical, and corrosion resistance properties of the films were analyzed using X-ray diffraction (XRD), atomic force microscopy (AFM), scanning electron microscopy (SEM), and electrochemical polarization measurements. Employing the atomic force microscope, morphological characteristics of CrN films under constant conditions using Minkowski Functionals were analyzed. Subsequently, by examining parameters like root-mean-square roughness, skewness, and kurtosis, the study revealed the variations in the particle distribution and their probability density as a function of deposition power alterations. Results indicate that increasing RF power enhances film crystallinity, as evidenced by intensified XRD peaks. With an increase in RF power reaching up to 80 W, there is a significant enhancement in the uniformity of the surface, resulting in an evenly spread grain pattern. Corrosion tests in 0.5 M sulfuric acid, assessed via potentiodynamic polarization, identify 80 W as the optimal RF power for maximizing corrosion resistance. This study provides insights into the relationship between film structure, surface characteristics, and corrosion behavior, highlighting key factors influencing protective performance.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 1","pages":"120-133"},"PeriodicalIF":2.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.70059","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144847644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0