Microscopy Research and Technique最新文献

{"title":"Viscoelasticity and Its Influence on the Manipulation of DNA Molecules","authors":"Xia Wang,&nbsp;Jianjun Dong,&nbsp;Ying Wang,&nbsp;Mingyan Gao,&nbsp;Fengyan Hou,&nbsp;Jingyu Wang,&nbsp;Ye Li,&nbsp;Zuobin Wang","doi":"10.1002/jemt.70004","DOIUrl":"10.1002/jemt.70004","url":null,"abstract":"<div>\u0000 \u0000 <p>The viscoelasticity of DNA molecules in the air were investigated using atomic force microscopy (AFM). For the individual DNA, compared to the randomly-coiled DNA, the stretched DNA showed smaller viscoelasticity, measured in the AFM quantitative imaging (QI) mode. The manipulation of DNA was performed, and it was influenced by its viscoelasticity. Compared to the stretched DNA, significant highlighted accumulations of DNA and lateral dragging tails were more obvious for the randomly-coiled DNA, which was caused by the elastic recoil and adhesion effects. In addition, the viscoelasticity of DNA aggregates composed of multiple DNA strands were measured. Compared to the individual DNA, they showed larger viscoelasticity. This study serves as a reference for the studies of DNA viscoelasticity-related behaviors in the air and has potential in gene knockout and targeted gene editing.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2733-2743"},"PeriodicalIF":2.1,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacognostic Identification and Quality Evaluation of Ferula ferulaeoides (Steud.) Korov.—An Ethnomedicinal Plant","authors":"Rong Guo,&nbsp;XueRui Zhang,&nbsp;FangWei Diao,&nbsp;Kai Liao,&nbsp;Yun Zhu","doi":"10.1002/jemt.70008","DOIUrl":"10.1002/jemt.70008","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 <i>Ferula ferulaeoides</i> (Steud.) Korov. (FFK) is used in ethnomedicine to treat gastrointestinal diseases. However, the lack of systematic quality control standards has hindered its market distribution and clinical application. The purpose of this study was to carry out pharmacognostic identification and quality evaluation of FFK for the establishment of quality control standards. The methods included organoleptic evaluation, microscopic observation, proximate analyses, qualitative chemical analysis, thin-layer chromatography (TLC) fingerprinting, and high-performance liquid chromatography (HPLC). The leaves of FFK were light green, and the roots were fusiform and thick. The resin was milky white in its fresh state, and it became a yellowish-brown irregular mass after air drying. Under the microscope, the secondary structure of the root was developed, and the resin ducts were mainly distributed in the cortex. The powder contained vessels, oil cells, fibers, starch granules, parenchyma cells, resin ducts, and stone cells, but the resin powder contained only resin mass. The ash, moisture, extract, and heavy metal contents in FFK complied with general standards of similar medicinal materials in the Chinese Pharmacopeia (ChP). Qualitative chemical analysis showed that FFK contained terpenoids, flavonoids, coumarins, and volatile oils. A TLC fingerprint was established for FFK. HPLC showed that the ferulic acid content in FFK was 0.5518 mg g⁻¹. This study preliminarily established the pharmacognostic identification standard for FFK and, combined with quality evaluation, could lay a foundation for its comprehensive development and utilization.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2721-2732"},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to “Unveiling Thrips Morphology: A Comparative Analysis of Microscopy Based Ultrastructural, Morphological, and Molecular Characterization of Thrips tabaci and Thrips parvispinus in Onion”","authors":"","doi":"10.1002/jemt.24880","DOIUrl":"10.1002/jemt.24880","url":null,"abstract":"&lt;p&gt;Arunkumar Pandi, Kannan Malaichamy, Kennedy John Samuel, et al., “Unveiling Thrips Morphology: A Comparative Analysis of Microscopy-Based Ultrastructural, Morphological, and Molecular Characterization of &lt;i&gt;Thrips tabaci&lt;/i&gt; and &lt;i&gt;Thrips parvispinus&lt;/i&gt; in Onion,” Microscopy Research and Technique 87, (2024): 3003–3015, https://doi.org/10.1002/jemt.24653.&lt;/p&gt;&lt;p&gt;In the originally published article, there are several changes to the text and figures. They are detailed below.&lt;/p&gt;&lt;p&gt;\u0000 &lt;b&gt;Abstract original text:&lt;/b&gt;\u0000 &lt;/p&gt;&lt;p&gt;\u0000 \u0000 &lt;b&gt;ABSTRACT&lt;/b&gt;\u0000 The present study unveils the intricate details on the morphology of thrips through optical, Field Emission Scanning Electron Microscopy (FE-SEM) and mitochondrial COI gene based molecular identification tools. The variation in the morphological characters &lt;i&gt;viz&lt;/i&gt;., antennae (7-segmented with forked sensorium on 3rd, 4th segments), ctenidia (paired ctenidia was present in 5-8 abdominal segments laterally), pronotum (2 pairs of posteroangular setae) were observed in both &lt;i&gt;Thrips tabaci and Thrips parvispinus&lt;/i&gt;, respectively. Similarly, ocelli colour (brown and red coloured), ocellar setae 2 and 3 pairs of ocellar setae on the head of &lt;i&gt;T. tabaci&lt;/i&gt; and &lt;i&gt;T. parvispinus&lt;/i&gt;, respectively; irregular reticulate striations on metascutum and medial striations are present in the metanotum of &lt;i&gt;T. parvispinus&lt;/i&gt;; forewings with 6 distal setae in the first vein and 15 distal setae in the second vein in &lt;i&gt;T. tabaci&lt;/i&gt; and forewings of &lt;i&gt;T. parvispinus&lt;/i&gt; with complete rows of setae in the 1st and 2nd vein in &lt;i&gt;T. parvispinus&lt;/i&gt;; abdomen with median dorsal setae present in the tergite of &lt;i&gt;T. tabaci&lt;/i&gt; and presence of 6–12 discal setae in sternites III-VI segments, absence of discal setae on sternites II &amp; VII in &lt;i&gt;T. parvispinus&lt;/i&gt; were observed respectively. Further, FE-SEM studies revealed that similar type of sensilla viz., sensilla basiconica (SBI, SBII, SBIII), sensilla chaetica (SchI, SChII), sensilla trichodea (ST), sensilla campaniformia (SCa), and sensilla cavity (SCav) were recorded in both the species and variations were observed in length of above sensilla of &lt;i&gt;T. tabaci&lt;/i&gt; and &lt;i&gt;T. parvispinus&lt;/i&gt;. Additionally, Bohm bristles (Bb) and microtrichia (Mt) on the antennal surface contributed to a comprehensive understanding of their ultrastructural features. The molecular characterization revealed a single ~450bp nucleotide fragment with over 98% similarity for the confirmation of &lt;i&gt;T. tabaci&lt;/i&gt; and &lt;i&gt;T. parvispinus&lt;/i&gt; in concurrence with NCBI data”. was incorrect.&lt;/p&gt;&lt;p&gt;\u0000 &lt;b&gt;This should have read:&lt;/b&gt; The present study unveils the intricate details on the morphology of thrips through optical, field emission scanning electron microscopy (FE-SEM) and mitochondrial COI gene-based molecular identification tools. The variation in the morphological characters namely, antennae (7-segmented with forked sensorium on 3r","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2843-2846"},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.24880","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiopacity of Universal Flowable Composite Resins: A Comparative Evaluation","authors":"Busra Ozdemir,&nbsp;Seyit Bilal Ozdemir","doi":"10.1002/jemt.70003","DOIUrl":"10.1002/jemt.70003","url":null,"abstract":"<div>\u0000 \u0000 <p>This study evaluates the radiopacity of eight universal flowable composite resins and one condensable universal composite resin using digital radiography, comparing their values with enamel and dentin in terms of equivalent aluminum thickness. Twenty-seven disc-shaped specimens (8 mm diameter and 2 mm thickness) were prepared, including one condensable universal composite resin (Filtek Z250) and eight universal flowable resins (Vittra Unique Flow, Neo Spectra ST-Flow, Omnichroma Flow, G-aenial Universal Flo, G-aenial Universal Injectable, Estelite Universal Flow Superlow, Estelite Universal Flow Medium, Estelite Universal Flow High). ImageJ software was used to calculate the mean gray values (MGV) of the specimens and enamel and dentin. EDS and SEM images (×10,000) were obtained. Statistical analysis included the Shapiro–Wilk test and Welch's ANOVA (<i>p</i> = 0.05). Results showed that all universal resins exhibited significantly higher MGV and mm Al values than dentin. Filtek Z250 and Neo Spectra ST-Flow had significantly higher values than enamel, whereas Vittra Unique Flow and G-aenial Universal Injectable displayed values similar to enamel. Other tested composites had MGV and mm Al values between those of enamel and dentin. EDS confirmed that the composites contained at least two radiopaque elements. Despite differences in radiopacity among the universal resins, all tested materials met the radiopacity criteria established by the International Organization for Standardization (ISO).</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2712-2720"},"PeriodicalIF":2.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Alterations of Ni-Ti Files After Retreatment of Root Canals Filled With Different Sealers: AFM and SEM Study","authors":"Duygu Aksoy,&nbsp;Sibel Koçak,&nbsp;Mustafa Murat Koçak,&nbsp;Baran Can Sağlam","doi":"10.1002/jemt.24884","DOIUrl":"10.1002/jemt.24884","url":null,"abstract":"<div>\u0000 \u0000 <p>The purpose of this in vitro study is to examine the processing of the surfaces of the files after removing the gutta percha in the root canals with different file systems. Eighteen mandibular molar teeth were used. The access cavities were prepared, the mesiobuccal canals were shaped and filled with the lateral condensation. During filling, AH Plus and Bioceramic sealers were used. The root canal fillings were removed using ProTaper Universal Retreatment (D1, D2, D3), Reciproc Blue file (R40), and TF Adaptive rotary file (SM2). Surface changes of the files after the removal of the root canal filling material in the canals were examined under Atomic Force Microscope (AFM) and Scanning Electron Microscope (SEM). The analysis revealed that the type of sealer used, whether resin or bioceramic, did not have a significant impact on the surface of the files (<i>p</i> &gt; 0.05). ProTaper Universal Retreatment (PTUR) instruments showed significantly less Ra and RMS values compared to the other files. Additionally, a significant difference was found between RCP Blue and TFA files in Ra and RMS values (<i>p</i> &lt; 0.05). PTUR instruments demonstrated better surface values regardless of the root canal sealer.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2704-2711"},"PeriodicalIF":2.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlative Microscopy Study of Phytoaccumulation of Widely Distributed Iron Oxide Colloidal Particles in Sprouts Exposed to Effluents","authors":"Alexandre Loukanov,&nbsp;Velichka Arahangelova","doi":"10.1002/jemt.24885","DOIUrl":"10.1002/jemt.24885","url":null,"abstract":"<div>\u0000 \u0000 <p>This study employs a correlative microscopy approach to investigate the uptake, translocation, and phytoaccumulation of widely distributed citric acid-stabilized iron oxide colloidal particles in red radish (\u0000 <i>Raphanus sativus</i>\u0000 ) sprouts. By integrating light and electron microscopy, this method provides a comprehensive, time-resolved analysis of nanoparticles accumulation within plant roots and their biosorption within a dynamically formed biofilm surrounding the root surface. Red radish sprouts were selected as an ideal model due to their rapid germination, well-developed fibrous root system, and high sensitivity to environmental changes, making them suitable for studying nanoparticle-plant interactions. To assess the role of microbial activity in nanoparticle transformation and bioavailability, a carbon nanodot fluorescence quenching technique was employed as a sensitive sensor-based approach to detect and monitor dissolved iron ions in colloidal suspensions. Results confirmed that no free iron ions were released into the effluent, indicating that uptake occurred exclusively in nanoparticulate form. The light microscopic images demonstrated that the biofilm matrix around the roots acted as a retention and filtration system, selectively adsorbing and concentrating colloidal particles near the root surface. Additionally, cross-sectional root tissue analysis using scanning electron microscopy (SEM) revealed that the smallest nanoparticles were able to penetrate plant tissues and undergo intracellular phytoaccumulation. Further energy-dispersive X-ray spectroscopy (EDS) mapping showed an exponential-like time-dependent increase in iron accumulation within root tissues, suggesting a pattern primarily driven by passive diffusion along concentration gradients. These findings provide novel insights into the mechanisms governing nanoparticle uptake in plants, emphasizing the role of plant-microbe interactions in modulating colloidal bioavailability. By elucidating the dynamics of nanoparticle accumulation and retention in plant-microbe systems, this study advances our understanding of phytoremediation processes and offers valuable implications for sustainable environmental remediation and precision agriculture applications.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2690-2703"},"PeriodicalIF":2.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and Histopathological Impact of DMBA-Induced Breast Cancer and Therapeutic Potential of Silk Sericin and Sericin Silver Nanoparticles","authors":"Samaira Mumtaz,&nbsp;Shaukat Ali,&nbsp;Asim Pervaiz,&nbsp;Farooq Ahmad,&nbsp;Asma Abdul Latif,&nbsp;Mah Noor Khan,&nbsp;Muhammad Khalil Ahmad Khan,&nbsp;Muhammad Adeeb Khan,&nbsp;Ali Hassan,&nbsp;Rizwan Ullah,&nbsp;Tafail Akbar Mughal,&nbsp;Saiqa Andleeb,&nbsp;Hafiz Muhammad Tahir","doi":"10.1002/jemt.24882","DOIUrl":"10.1002/jemt.24882","url":null,"abstract":"<div>\u0000 \u0000 <p>Nanotechnology is an emerging field in the therapeutic domain of cancer research. The development of novel anticancer agents in combination with nanotechnology is a highly promising area as it could offer safer and more effective delivery methods for better overall outcomes. Considering this, a naturally occurring silkworm protein (sericin) was combined with nanoparticle formulations to explore the subsequent anticancer effects in breast cancer. In this study, a breast cancer animal mice model was developed by using 7, 12-dimethylbenzneanthracene (DMBA) (a carcinogenic compound) followed by evaluation of the antitumor effects of a natural protein (sericin) and its nanoparticle conjugates. For this purpose, the mice were treated with two different concentrations of individual sericin protein together with conjugated silver nanoparticles (100–200 mg/kg, b.w.). Mice were dissected after 60 days of the treatment, and biochemical results showed that sericin and its conjugated nanoparticles had significant effects against the DMBA-induced carcinogenic group in comparison with the untreated control group. Particularly, sericin-conjugated NPs induced more prominent effects in the animal model. The histopathological analysis of the spleen and mammary tissues showed cytotoxic effects in the DMBA group, and sericin-conjugated NPs were highly effective and minimized the cytotoxic effects of DMBA. Consequently, sericin-conjugated nanoparticles induced more efficient anti–breast tumor effects in animal mice models and have promising future clinical applications in overcoming drug resistance.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2675-2689"},"PeriodicalIF":2.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphoanatomic, Molecular, and Scanning Electron Microscopy Characterization of Two New Itajahya Species (Basidiomycota, Phallales) From Kundian Forest, Punjab, Pakistan","authors":"Ayesha Bibi,&nbsp;Najam-ul-Sehar Afshan,&nbsp;Abdul Nasir Khalid","doi":"10.1002/jemt.24881","DOIUrl":"10.1002/jemt.24881","url":null,"abstract":"<div>\u0000 \u0000 <p>The genus <i>Itajahya</i>, a rare member of the Phallaceae family, is characterized by its unique calyptra at the apex of the gleba. During 2020–2024, we collected fresh specimens of two new <i>Itajahya</i> species from Pakistan, enabling DNA sequencing, thorough morphoanatomic, and scanning electron microscopic characterization. This study aims to document these species with detailed descriptions, DNA analyses, and comparisons to existing species, clarifying their taxonomic status. <i>Itajahya pakistanica</i> features a white to off-white immature fruiting body, dark greenish to blackish gleba, and a white to off-white calyptra, while <i>I. punjabensis</i> is distinguished by its pink to peach immature fruiting body, dark greenish to brown gleba, and a prominent pink calyptra exceeding the width of the cap. Stinkhorn fungi, including <i>Itajahya</i>, produce a gelatinous gleba with an unpleasant odor that attracts insects for spore dispersal. <i>Itajahya</i> produces volatile compounds, fatty acids, and pheromones that attract <i>Drosophila</i> species, aiding in their breeding and feeding on the gleba. Future studies will focus on identifying these compounds at different fruiting stages and their role in insect interactions and spore dispersal. Our findings have increased the number of known species in this genus to six. This study aims to stimulate further interest in the genus <i>Itajahya</i> and encourage the collection of additional specimens for DNA studies and phylogenetic analysis.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2664-2674"},"PeriodicalIF":2.1,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Atomic Force Microscope Photoelectric Sensing Circuit Based on Kalman Filter","authors":"Ke Xu,&nbsp;Honghao Qian","doi":"10.1002/jemt.24883","DOIUrl":"10.1002/jemt.24883","url":null,"abstract":"<div>\u0000 \u0000 <p>The noise performance of photoelectric sensing circuits hinders the performance development and intelligent application of atomic force microscopy systems. This article successfully developed an atomic force microscope photoelectric sensing circuit based on Kalman filtering. This article embeds the Kalman filter algorithm into the photoelectric sensing circuit to filter and denoise the output signal. This article uses the STM32 microcontroller to achieve analog-to-digital conversion, thereby reducing the attenuation and distortion of signal transmission. Specifically, the photoelectric sensing circuit has a detection limit of ±5 V, a fast response speed of about 10 us, and a laser wavelength detection range of 100–1100 nm. This method reduces the transmission attenuation of the output signal of the photoelectric sensing circuit. It improves the signal-to-noise ratio of the output signal without interfering with the performance of the photoelectric sensor itself. In addition, for the dynamic atomic force microscope system, this paper combines the photoelectric sensing circuit with negative feedback control to improve the negative feedback detection method of the dynamic working mode.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2647-2663"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144192018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Florivory on the Anatomy, Histochemistry and Resource Production of Flowers of Senna aversiflora (Herb.) H.S. Irwin and Barneby","authors":"Edinalva Alves Vital dos Santos,&nbsp;Emília de Arruda,&nbsp;Ana Virgínia Leite,&nbsp;Natan Messias Almeida","doi":"10.1002/jemt.24779","DOIUrl":"10.1002/jemt.24779","url":null,"abstract":"<div>\u0000 \u0000 <p>Florivory directly affects floral structures, especially petals and anthers. The physical damage to these whorls can alter the characteristics of the flowers, compromise their functions, and consequently impact fertility and reduce the reproductive success of the species. We provide the floral anatomical description of <i>Senna aversiflor</i>a (Herb.) H.S. Irwin and Barneby. We measured various anatomical traits of petals and quantified the levels of chemical compounds and the pollen produced by intact and damaged flowers in order to identify characters associated with the plant-florivore interaction. We found that the epidermis (adaxial and abaxial surfaces) and mesophyll of the petals of healthy flowers were thicker when compared to damaged flowers. We infer that the smaller thickness of traits associated with the absence of characters with deterrent effect on herbivores and greater production of attractive/nutritive chemical compounds in relation to defense compounds contribute to making the species highly susceptible to florivory. Pollen production in damaged flowers did not differ between the different stages of floral development. However, florivory has a negative effect on the amount of pollen produced. Damaged flowers had less pollen than healthy flowers. We conclude that florivory in <i>S. aversiflora</i> exerts significant pressure on petal anatomy and resource production by flowers.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 10","pages":"2630-2646"},"PeriodicalIF":2.1,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}