Synthesis of New Copolymer and Genotoxic Effects on Triticum aestivum L. (Wheat) Root Tip Cells.

Abstract

In the present study, a copolymer of poly(N,N-dimethylacrylamide) and sodium acrylate was synthesized with the Solution Polymerization Technique. The purpose was to uncover the cytotoxic and genotoxic effects on root tip cells of the Triticum aestivum L. plant germinated with this polymer at different concentrations (25, 50, 100, and 200 ppm). The deposition of poly(DMAA-co-NaAc) copolymer in the study sample T. aestivum was demonstrated with the FTIR, UV-VIS spectroscopy analysis, X-Ray Diffraction (XRD) Spectroscopy, Scanning Electron Microscopy (SEM), SEM Element Map, and Energy Dispersive Spectroscopy (EDS) Spectrum. All concentrations of the poly(DMAA-co-NaAc) copolymer caused a decrease in the Mitotic Index (MI). The poly(DMAA-co-NaAc) copolymer caused various mitotic abnormalities such as loss of genetic material, deconstructed prophase, adhesion, chromosome groupings in metaphase, deconstructed metaphase, C-metaphase, chromosomal loss, chromosomal fracture, deconstructed anaphase, lagging chromosome, fragment, polar aberration, bridge, advancing, star anaphase, multipolarity, and deconstructed telophase. Study results show that poly(DMAA-co-NaAc) copolymer will cause abnormalities in mitosis as a result of its uptake by plants. These results also show that the poly(DMAA-co-NaAc) polymer, which affects organisms, must be produced in a controlled setting to reduce its accumulation in nature and ultimately disposed of in a way that will not harm the environment.