Morphology and Viability Study of IHOEC Ovarian Epithelial Cells on PAN/PPy Electrospun Nanofibrous Scaffold.

Abstract

Electrospun nanofibers offer sufficiently large pore size, good porosity, and interconnectivity for the integration of cells into the scaffold. Consequently, there has been a lot of interest in tissue engineering with electrospun nanomaterials. Numerous cellular functions can be supported by biocompatible conducting polymers. In this study, polyacrylonitrile (PAN)/polypyrrole (PPy) nanofibrous films were prepared by the electrospinning technique and examined as a scaffold for IHOEC ovarian cell attachment and proliferation. The rationale for embedding ovarian cells in a scaffold is particularly relevant to fields such as ovarian tissue engineering, infertility treatments, and postcancer tissue repair. This approach aims to enable cells to grow and form functional tissue in an environment similar to their natural microenvironment. Straight, smooth, and beadless PAN/PPy nanofibers with an average diameter of 216 ± 35 nm were obtained and analyzed by AFM, SEM, TEM, TGA, XRD, and WCA characterization methods. WCA measurements revealed that the nanofibers exhibited hydrophilic behavior, with WCA values of 14.93° ± 0.37 and 12.51° ± 0.50 being measured for the PAN and PAN/PPy nanofibers, respectively. PAN/PPy nanofibrous scaffolds were examined as a tissue engineering scaffold material for IHOEC ovarian cells, and the morphological properties and viability of cells grown on PAN/PPy nanofibers were observed. Results from the MTT test and SEM pictures demonstrated that IHOEC cells could adhere and proliferate on nanofibers. Consequently, PAN/PPy nanofibrous mats would be a potential candidate for an ovarian tissue scaffold.