Microscopy Research and Technique最新文献

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Immobilization of Nickel Oxide Nanoparticles on Cotton Fiber Under Gamma Radiation and Its Catalytic Activity.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-11 DOI: 10.1002/jemt.24818
Wael H Eisa, A M Abou Zeid, O A Ghazy, S A Waly, H H Saleh, Z I Ali
{"title":"Immobilization of Nickel Oxide Nanoparticles on Cotton Fiber Under Gamma Radiation and Its Catalytic Activity.","authors":"Wael H Eisa, A M Abou Zeid, O A Ghazy, S A Waly, H H Saleh, Z I Ali","doi":"10.1002/jemt.24818","DOIUrl":"https://doi.org/10.1002/jemt.24818","url":null,"abstract":"<p><p>Nickel ions were reduced on the surface of cotton fibers under the effect of gamma radiation. The NiO nanoparticles grew as semispherical aggregates along the cotton fibers with average crystallite size of 32 nm as calculated from the x-ray diffraction results. The FTIR data showed the presence of a new IR peak at 613 cm<sup>-1</sup> (assigned to the NiO stretching vibration) confirming the successful loading of NiO nanoparticles into cotton matrix. The NiO nanoparticles were adhered to the surface of cotton fibers through the complexation with hydroxyl and carbonyl groups. The NiO@cotton nanocomposite showed high performance in the catalytic degradation of both 4-nitrophenol and bromophenol blue. The rate constant of the catalytic degradation of 4-nitrophenol was 8.24 × 10<sup>-3</sup> s<sup>-1</sup> at pH = 10. The resultant heterogeneous catalyst is green, cheap, separable/recyclable, and has considerable catalytic activity.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pichia kudriavzevii (JD2) Immobilized on Acid Activated Perlite as a Biosorbent for Solid Phase Extraction of Cr(III) Determination by AAS (Atomic Adsorption Spectroscopy).
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-11 DOI: 10.1002/jemt.24815
Aliya Amanzhol, Özcan Yalçınkaya, Berat Çinar Acar, Zehranur Yuksekdag
{"title":"Pichia kudriavzevii (JD2) Immobilized on Acid Activated Perlite as a Biosorbent for Solid Phase Extraction of Cr(III) Determination by AAS (Atomic Adsorption Spectroscopy).","authors":"Aliya Amanzhol, Özcan Yalçınkaya, Berat Çinar Acar, Zehranur Yuksekdag","doi":"10.1002/jemt.24815","DOIUrl":"https://doi.org/10.1002/jemt.24815","url":null,"abstract":"<p><p>In this study, the preconcentration of Cr(III) ion in Pichia kudriavzevii JD2 immobilized perlite adsorbent by solid phase extraction was investigated. The determination of Cr(III) was performed using flame atomic absorption spectrometry (AAS). The effects of pH, adsorbent amount, recovery solution volume and type, and sample solution flow rate and volume on the recovery efficiency of Cr(III) ions were investigated. Optimized preconcentration conditions for Cr(III) were established using a column technique. The optimal parameters were determined as pH 4, a recovery solution of 2 mol/L HNO<sub>3</sub> with a volume of 10 mL, and a sample flow rate of 1-3 mL/min, preconcentration factor 25. Under these conditions, the recovery efficiency of Cr(III) ion on perlite immobilized with Pichia kudriavzevii JD2 was found to be 100.1% ± 0.3% with a 95% confidence level. Analytical variables with a limit of detection (LOD) of 4.8 μg/L and a limit of quantification (LOQ) of 15.8 μg/L were determined for the Cr(III) ion. The accuracy of the method was determined using standard reference materials (SPS-WW1). The relative error of the recovery efficiency was determined to be less than 10%. The method was applied to the determination of Cr(III) in various water samples, such as tap water and mineral waters.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
UHPLC-QTOF-MS-Based Metabolic Profiling, Estimation of Antioxidants and Anticancer Potential Assessment of Dicliptera bupleuroides Nees by Caspase Activation, and ROS and MMP Evaluation by Using Fluorescence Microscopy.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-05 DOI: 10.1002/jemt.24811
Mehak Sarfraz, Fiaz Alam, Zia Uddin
{"title":"UHPLC-QTOF-MS-Based Metabolic Profiling, Estimation of Antioxidants and Anticancer Potential Assessment of Dicliptera bupleuroides Nees by Caspase Activation, and ROS and MMP Evaluation by Using Fluorescence Microscopy.","authors":"Mehak Sarfraz, Fiaz Alam, Zia Uddin","doi":"10.1002/jemt.24811","DOIUrl":"https://doi.org/10.1002/jemt.24811","url":null,"abstract":"<p><p>Dicliptera bupleuroides has been used traditionally for treating various ailments in many countries. Its detailed phytochemical profiling and pharmacological evaluation in treating cancer based on reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) analysis along with caspase activation studies has not been done previously. This study was conducted for detailed phytochemical profiling of D. bupleuroides by using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) and to evaluate its antioxidant and anticancer potential against hepatocellular carcinoma (HepG2) cells. The extract was subjected to UHPLC-QTOF-MS analysis for metabolite profiling, and GNPS Molnet enhancer determined its major phytochemical classes. Phytochemical tests and multimode antioxidant assays were also executed. The extract was pharmacologically evaluated for its anticancer potential against HepG2 cells using CCK-8 assay. Intracellular ROS generation and loss in MMP were studied by fluorescence microscopy. Network pharmacology and molecular docking of selected compounds were executed. The extract contains total phenolic content as 58.37 ± 0.32 μg GAE/mg extract, and remarkable antioxidant potential was revealed in total antioxidant contents (TAC) and total reducing power (TRP) assays, i.e., 70.4 ± 0.4, 83.2 ± 1.9 μg ascorbic acid equilant (AAE) Borges/mg extract. The UHPLC-QTOF-MS analysis showed that the extract contains flavonoid glycosides, phenolic acids, flavans, O-methylated flavonoids, linoleic acids, terpene glycosides, triterpenoid saponins, and certain other phytoconstituents. Maximum inhibition of 77.13% ± 1.60% against HepG2 cells was observed at 24 h. Loss in MMP by JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimi- dazolylcarbocyanine iodide) staining and increased ROS generation, and caspase-3, caspase-8 activation by extract significantly illustrated the cancer cell death by apoptosis. D. bupleuroides could be a potential source of safe and therapeutically active compounds that can be purified to develop safer, selective, and efficacious anticancer agents with fewer side effects.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biosilica 3D Micromorphology of Geodiidae Sponge Spicules Is Patterned by F-Actin.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-02 DOI: 10.1002/jemt.24798
Alona Voronkina, Paco Cárdenas, Jörg Adam, Heike Meissner, Krzysztof Nowacki, Yvonne Joseph, Konstantin R Tabachnick, Hermann Ehrlich
{"title":"Biosilica 3D Micromorphology of Geodiidae Sponge Spicules Is Patterned by F-Actin.","authors":"Alona Voronkina, Paco Cárdenas, Jörg Adam, Heike Meissner, Krzysztof Nowacki, Yvonne Joseph, Konstantin R Tabachnick, Hermann Ehrlich","doi":"10.1002/jemt.24798","DOIUrl":"https://doi.org/10.1002/jemt.24798","url":null,"abstract":"<p><p>Demosponges (phylum Porifera) are among the first multicellular organisms on the planet and represent a unique archive of biosilica-based skeletal structures with species-specific microstructures called spicules. With more than 80 morphotypes, this class of sponges is recognized as a unique source of amorphous silica with superficial ornamentation patterned by organic phases. In this study, we investigated spicules of selected representatives of the family Geodiidae (order Tetractinellida), to identify F-actin-containing axial filaments within these 3D skeletal microconstructs defined as oxyspherasters and sterrasters. Their desilicification using 10% HF leads to isolation of multifilamentous, radially oriented organic matrices, which resemble the shape and size of the original spicules. Our data show that highly specific indicators of F-actin such as iFluorTM 594-Phalloidin, iFluorTM 488-Phalloidin, as well as iFluorTM 350-Phalloidin unambiguously confirm its localization within demineralized oxyspherasters and sterrasters of 11 diverse demosponges species belonging to the subfamily Geodiinae (genera Geodia, Rhabdastrella) and the subfamily Erylinae (genera Caminella, Caminus, Erylus, Pachymatisma). Well-defined periodicity in Geodia cydonium sterrasters actin filaments has been observed using atomic force microscopy (AFM) for the first time. The findings of F-actin as a possible pattern driver in spicules of geodiids brings additional light to our knowledge of spiculogenesis in this group. However, no specific actin structures were found between the geodiid subfamilies or genera thereby suggesting a common actin process, present already at the emergence of the family (~170 million years ago).</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PathoCoder: Rethinking the Flaws of Patch-Based Learning for Multi-Class Classification in Computational Pathology.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-02 DOI: 10.1002/jemt.24800
Ferdaous Idlahcen, Pierjos Francis Colere Mboukou, Ali Idri, Hicham El Attar
{"title":"PathoCoder: Rethinking the Flaws of Patch-Based Learning for Multi-Class Classification in Computational Pathology.","authors":"Ferdaous Idlahcen, Pierjos Francis Colere Mboukou, Ali Idri, Hicham El Attar","doi":"10.1002/jemt.24800","DOIUrl":"https://doi.org/10.1002/jemt.24800","url":null,"abstract":"<p><p>Pathology-based decision support systems in clinical settings have faced impediments from data preparation beforehand, large-scale manual annotations, and poor domain generalization. We report a unified hybrid framework with only raw, slide-level label images. The method, which we termed PathoCoder, comprises core feature extractors, a feature combiner/reduction, and a supervised classifier. It is trained (through 5-fold cross-validation) on 2452 SurePath cervical liquid-based whole-slide captures, provided from Mendeley repository. Tests resulted in 98.37%, 98.37%, 98.41%, and 98.37% in accuracy, precision, recall, and F1, respectively. Extensive experiments validate the proposed scheme and versatility enough to accommodate epithelial ovarian tumor histotypes. Our method paves the way for more accelerated advancements in pathology AI by reducing patch/pixel-based annotation and good tissue quality dependency. Its applicability spans diverse classification tasks with varying tissue content and holds potential for real-world implementation.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Visualization of Unhatched Brine Shrimp Eggs in Zebrafish Intestines Using Synchrotron Radiation Phase-Contrast CT.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-02 DOI: 10.1002/jemt.24814
Rongbiao Tang, Feixiang Wang, Ke Li, Yi Chen, Yanan Fu, Ke-Min Chen
{"title":"Visualization of Unhatched Brine Shrimp Eggs in Zebrafish Intestines Using Synchrotron Radiation Phase-Contrast CT.","authors":"Rongbiao Tang, Feixiang Wang, Ke Li, Yi Chen, Yanan Fu, Ke-Min Chen","doi":"10.1002/jemt.24814","DOIUrl":"https://doi.org/10.1002/jemt.24814","url":null,"abstract":"<p><p>Brine shrimp are an ideal bait for zebrafish with high protein content. In laboratory settings, brine shrimp eggs (BSEs) are commonly used to hatch for acquiring brine shrimp. However, not all BSEs are able to successfully hatch into brine shrimp. Actually, some unhatched BSEs (UBSEs) retain and are collected along with the brine shrimp as bait. The digestibility of UBSEs and their potential impact on the digestive system of zebrafish have not been demonstrated. In this study, high-resolution synchrotron radiation phase-contrast CT (PCCT) was used to show the internal structures of UBSEs. Morphological changes of UBSEs were investigated after digestion in zebrafish intestines. The visualization of shell rupturing, collapsing, and shattering of UBSEs reveals that zebrafish intestines exerted much extra useless effort to digest the UBSEs. The results indicate that UBSEs should be completely discarded from the bait of brine shrimp before feeding zebrafish.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhanced Photocatalytic Degradation of Bisphenol A by a Novel MOF/CuFe2O4 Composite in Wastewater Treatment.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-02-02 DOI: 10.1002/jemt.24813
Khalid Javed, Muhammad Bilal, Naseem Abbas, Sajid Mahmood, Tanzeela Fazal, Shahid Iqbal, Khalid M Alotaibi
{"title":"Enhanced Photocatalytic Degradation of Bisphenol A by a Novel MOF/CuFe<sub>2</sub>O<sub>4</sub> Composite in Wastewater Treatment.","authors":"Khalid Javed, Muhammad Bilal, Naseem Abbas, Sajid Mahmood, Tanzeela Fazal, Shahid Iqbal, Khalid M Alotaibi","doi":"10.1002/jemt.24813","DOIUrl":"https://doi.org/10.1002/jemt.24813","url":null,"abstract":"<p><p>The synergistic effects of a CuFe₂O₄ and cobalt/nickel metal organic framework (Co/Ni-MOF) based composite (MOF/CuFe₂O₄) were explored for photodegradation of Bisphenol A (BPA), various MOF/CuFe₂O₄ composites were synthesized via a hydrothermal method, By adjusting CuFe₂O₄ to Co/Ni-MOF mass ratios of 2:1, 1:1, and 1:2 and were denoted as MOF/CuFe₂O₄ (2:1), MOF/CuFe₂O₄ (1:1), and MOF/CuFe₂O₄ (1:2), respectively. The composite MOF/CuFe₂O₄ (1:1) with a band gap energy (Eg) of 2.28 eV exhibited excellent photocatalytic activity achieving 98% degradation of a 10 ppm BPA solution under visible light (50 W) irradiation within 75 min, at pH 3, 25°C. This process achieved a quantum yield (QY) of 9.10 × 10<sup>-6</sup> molecules photon<sup>-1</sup> and a space-time yield (SY) of 9.10 × 10<sup>-7</sup>, highlighting the composite's efficiency and potential for practical applications. Visible-light absorption efficiency improved as photon energy increased (25 to 50 W) and facilitated the generation of <math> <semantics><mrow><mo>˙</mo> <msubsup><mi>O</mi> <mn>2</mn> <mo>-</mo></msubsup> </mrow> <annotation>$$ dot{mkern6mu}{mathrm{O}}_2^{-} $$</annotation></semantics> </math> radicals. Kinetic studies indicated a first-order reaction rate (R<sup>2</sup> = 0.964) for BPA photodegradation by MOF/CuFe₂O₄ (1:1) composite. Additionally, the MOF/CuFe₂O₄ composite demonstrated superior antimicrobial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) under light compared with dark environment. Remarkably, the composite maintained its photocatalytic efficiency over at least six cycles. The results of the current study highlight the effectiveness and reusability of the MOF/CuFe₂O₄ (1:1) composite as a nanomaterial for the photodegradation of BPA and its potential applications in water treatment.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimizing Skin Cancer Diagnosis: A Modified Ensemble Convolutional Neural Network for Classification.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-01-31 DOI: 10.1002/jemt.24792
A M Vidhyalakshmi, M Kanchana
{"title":"Optimizing Skin Cancer Diagnosis: A Modified Ensemble Convolutional Neural Network for Classification.","authors":"A M Vidhyalakshmi, M Kanchana","doi":"10.1002/jemt.24792","DOIUrl":"https://doi.org/10.1002/jemt.24792","url":null,"abstract":"<p><p>Skin cancer is recognized as one of the most harmful cancers worldwide. Early detection of this cancer is an effective measure for treating the disease efficiently. Traditional skin cancer detection methods face scalability challenges and overfitting issues. To address these complexities, this study proposes a random cat swarm optimization (CSO)with an ensemble convolutional neural network (RCS-ECNN) method to categorize the different stages of skin cancer. In this study, two deep learning classifiers, deep neural network (DNN) and Keras DNN (KDNN), are utilized to identify the stages of skin cancer. In this method, an effective preprocessing phase is presented to simplify the classification process. The optimal features are selected using the feature extraction phase. Then, the GrabCut algorithm is employed to carry out the segmentation process. Also, the CSO is employed to enhance the effectiveness of the method. The HAM10000 and ISIC datasets are utilized to evaluate the RCS-ECNN method. The RCS-ECNN method achieved an accuracy of 99.56%, a recall of 99.66%, a specificity value of 99.254%, a precision value of 99.18%, and an F1-score value of 98.545%, respectively. The experimental results demonstrated that the RCS-ECNN method outperforms the existing techniques.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microscopic Characterization Based Synthesis of Silver Nanoparticles Using Merremia quinquefolia (L.) Hallier f. Leaf Extract: Their Biological Approaches and Degradation of Methylene Blue Dye.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-01-29 DOI: 10.1002/jemt.24789
Arasangam Bharathi, Sivakumar Saipraba, Devaraj Ravichandran, Raji Meena, Palanisamy Prakash, Mansour K Gatasheh, Anis Ahamed, Subban Murugesan
{"title":"Microscopic Characterization Based Synthesis of Silver Nanoparticles Using Merremia quinquefolia (L.) Hallier f. Leaf Extract: Their Biological Approaches and Degradation of Methylene Blue Dye.","authors":"Arasangam Bharathi, Sivakumar Saipraba, Devaraj Ravichandran, Raji Meena, Palanisamy Prakash, Mansour K Gatasheh, Anis Ahamed, Subban Murugesan","doi":"10.1002/jemt.24789","DOIUrl":"https://doi.org/10.1002/jemt.24789","url":null,"abstract":"<p><p>The green methods for the synthesis of silver nanoparticles (AgNPs) has developed popularity recently due to the low preparation costs, environmental friendliness, and non-toxicity of the precursors. In this study, AgNPs were synthesized using leaf extract from Merremia quinquefolia. Spectroscopic techniques were used for analyzing the functional groups, morphology, crystalline phase, and elemental composition of nanomaterials. The ultraviolet (UV) visible spectrometry absorption spectra of the AgNPs had a surface plasmon resonance band at 459 nm and Fourier transformed infrared spectrum (FTIR) analysis showed the presence of elements groups. X-ray diffraction (XRD) analysis indicates the crystalline structure and the energy-dispersive x-ray spectroscopy (EDAX) analysis shows strong signals for the silver element. Morphological analysis using transmission electron microscope (TEM) and scanning electron microscopy (SEM) analyses revealed that the AgNPs exhibited spherical shapes with an average size of 14 nm. Furthermore, it was indicated by the Raman spectra vibrational peak at 240 and 470 cm<sup>-1</sup>. The disk diffusion method showed that AgNPs were highly effective in inhibition of Gram-positive bacteria Staphylococcus aureus with a high inhibitory zone (14 ± 0.23 mm). Antioxidant activity, the IC<sub>50</sub> values for assays DPPH (145.7 μg/mL) and ABTS (112.09 μg/mL), and albumin denaturation in human red blood cells showed higher anti-inflammatory activity. AgNPs had an IC<sub>50</sub> value of 4.62 μg/mL against breast cancer (MCF-7) cells. Methylene blue (MB) degrading studies were used to assess the photocatalytic activity of AgNPs. They are at 240 min observed sunlight, the MB degradation efficiency was 94.89%. Overall, although M. quinquefolia synthesizes AgNPs for environmentally friendly applications, the study is indicated to fully understand the potential involved in treating breast cancer cells.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143066657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photochemically Activated Microbubble Emulsion, Nd: Yap Laser, and Cerium Oxide Nanoparticles as Canal Disinfectants on Push-Out Bond Strength of Sealer to Root Dentin, Smear Layer, and Antibacterial Efficacy.
IF 2 3区 工程技术
Microscopy Research and Technique Pub Date : 2025-01-28 DOI: 10.1002/jemt.24802
Badi Alotaibi, Sarah Salah Gaafar, Roqayah Aljuailan, Heba Lutf Ahmed Alawadhi, Hanin Alsalhi, Hanan Alharbi, Renad Sultan Alfaraidy, Ahlam Ahmed Ali Smran, Fayez Hussain Niazi
{"title":"Photochemically Activated Microbubble Emulsion, Nd: Yap Laser, and Cerium Oxide Nanoparticles as Canal Disinfectants on Push-Out Bond Strength of Sealer to Root Dentin, Smear Layer, and Antibacterial Efficacy.","authors":"Badi Alotaibi, Sarah Salah Gaafar, Roqayah Aljuailan, Heba Lutf Ahmed Alawadhi, Hanin Alsalhi, Hanan Alharbi, Renad Sultan Alfaraidy, Ahlam Ahmed Ali Smran, Fayez Hussain Niazi","doi":"10.1002/jemt.24802","DOIUrl":"https://doi.org/10.1002/jemt.24802","url":null,"abstract":"<p><p>Effect of different root canal irrigation regimes microbubble emulsion (MBE) via riboflavin photosensitizer (RFP), cerium oxide (CeO<sub>2</sub>) nanoparticles (NPs), and Nd: YAP laser on antibacterial efficiency, microhardness (MH), smear layer (SL) removal efficacy, and push-out bond strength (PBS) of AH plus sealer to canal dentin. Sixty single-rooted teeth were selected, disinfected, and categorized into four groups based on the type of disinfection. Following disinfection, a pair of samples were randomly selected and visualized under scanning electron microscope (SEM) for SL evaluation. Three samples from each disinfection group were mounted on a Vickers MH tester for hardness testing. A brain-heart infusion (BHI) agar plate was inoculated with a single colony of Enterococcus faecalis (E. faecalis), and their survival rate was measured following different disinfecting regimes. The remaining samples were filled with gutta-percha (GP) and sectioned to evaluate PBS via universal testing machine (UTM), followed by failure analysis. Data were analyzed using analysis of variance (ANOVA) and Tukey's post hoc test. The survival rate of E. faecalis was lowest for Group 3 (0.11 CFU/mL) with the highest MH values (49.72 ± 1.02 N/mm<sup>2</sup>). The PBS was highest for Group 3 cervical (11.54 ± 0.49 MPa) and middle third (11.12 ± 0.50 MPa). SL removal efficacy was comparable in groups 3 and 4 at the coronal and middle third. The application of CeO<sub>2</sub>NPs as a canal irrigant in conjunction with EDTA resulted in enhanced bond values, demonstrating superior SL removal capabilities. This type of disinfection notably enhances the MH of canal dentin while demonstrating significant sterilization efficacy against E. faecalis.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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