Microscopy Research and Technique最新文献

{"title":"Accessibility of Acid Sites and Catalytic Performance of FCC Catalysts on Different Matrices Studied by Super-Resolution Fluorescence Microscopy","authors":"Qin Tang,&nbsp;Huan Wang,&nbsp;Li Zhang,&nbsp;Meihua Zhang,&nbsp;Lei Yu,&nbsp;Huimin Guan,&nbsp;Yucai Qin,&nbsp;Lijuan Song","doi":"10.1002/jemt.70108","DOIUrl":"10.1002/jemt.70108","url":null,"abstract":"<div>\u0000 \u0000 <p>The accessibility of acid sites in fluid catalytic cracking (FCC) catalysts is a decisive factor influencing their catalytic performance. This study selected three FCC catalysts with different matrices (CAT-1: pure kaolinite; CAT-2: 90 wt% kaolinite +10 wt% APM-7; CAT-3: 90 wt% kaolinite +10 wt% APM-9). By combining super-resolution single-molecule fluorescence microscopy (N-STORM), confocal microscopy, and multiple characterization techniques (SEM, N₂ physisorption, NH₃-TPD, Py-FTIR), we systematically investigated the influence mechanism of matrix structure on acid site accessibility and catalytic performance. For the first time, nanoscale visualization of acid sites on FCC catalyst surfaces was achieved through fluorescence oligomerization of thiophene probe molecules at Brønsted acid sites. The results demonstrate that CAT-2, owing to the incorporation of APM-7, exhibits optimal pore connectivity (mesopore volume: 0.179 cm³/g) and Brønsted acid density (0.199 mmol/g). This significantly enhances acid site accessibility, leading to a more than 2-fold improvement in thiophene conversion efficiency compared to CAT-1. This study not only provides a novel strategy for the rational design of FCC catalysts, but also demonstrates the unique advantages of super-resolution fluorescence microscopy in investigating active sites of heterogeneous catalysts, offering critical theoretical support for industrial catalyst optimization.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 7","pages":"1049-1059"},"PeriodicalIF":2.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Transscleral Approach to Obtain High Quality Non-Destructive Images of the Retinal Pigment Epithelium","authors":"Lucia Dominguez,&nbsp;Juan Etchart,&nbsp;Rodrigo M. Torres,&nbsp;Javier Adur","doi":"10.1002/jemt.70133","DOIUrl":"10.1002/jemt.70133","url":null,"abstract":"<div>\u0000 \u0000 <p>We present an innovative transscleral imaging technique to obtain high-quality, non-destructive images of the retinal pigment epithelium (RPE) of enucleated eyes. This approach combines confocal microscopy with fluorescence lifetime imaging to assess both morphological and fluorescence lifetime characteristics of the RPE in murine models. Unlike traditional transpupillary methods, our transscleral approach significantly reduces autofluorescence interference from the cornea and lens, providing clearer images and accurate fluorescence lifetime measurements. Using a continuous-wave 405 nm laser, we achieved high-resolution imaging of the RPE without the need for complex pulsed laser setups. Morphological characterization demonstrated that the transscleral approach effectively visualizes hexagonal, mono- and binucleated RPE cells with clear delineation. Fluorescence lifetime analysis using pulsed 405 nm laser revealed distinct autofluorescent signatures corresponding to putative endogenous fluorophores. Notably, fixed tissue samples showed altered fluorescence lifetimes compared to fresh samples, highlighting the influence of preservation on biochemical assessment. Quantitative fluorescence lifetime data revealed differences between autofluorescent granules and the surrounding cytoplasm, indicating the potential to obtain information about changes in the cellular chemical environment. Our results suggest that transscleral FLIM imaging is a viable tool for non-destructive RPE analysis, offering potential applications in studying retinal diseases.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 7","pages":"1016-1027"},"PeriodicalIF":2.1,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146227327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study on the Identification of Xanthium sibiricum Patrin ex Widder and Xanthium italicum Moretti Based on Three Microscopy Technology","authors":"Liqi Cao,&nbsp;Chang Lu,&nbsp;Anqi Wang,&nbsp;Jinyan Wang,&nbsp;Haitao Cheng,&nbsp;Xiaoqiu Liu,&nbsp;Xiajing Xu,&nbsp;Yingni Pan","doi":"10.1002/jemt.70120","DOIUrl":"10.1002/jemt.70120","url":null,"abstract":"<div>\u0000 \u0000 <p>\u0000 \u0000 <i>Xanthium sibiricum</i>\u0000 Patrin ex Widder and \u0000 <i>Xanthium italicum</i>\u0000 Moretti are morphologically similar fructus that are frequently misidentified. \u0000 <i>Xanthium italicum</i>\u0000 Moretti may possess inherent toxicity, and its adulteration of genuine medicinal materials poses a threat to clinical drug safety. Macroscopic observation and three microscopic techniques including stereo microscope, optical microscope, and 3D X-ray microscope were used for morphological identification of \u0000 <i>Xanthium sibiricum</i>\u0000 Patr ex Widder and \u0000 <i>Xanthium italicum</i>\u0000 Moretti in this study. 3D X-ray microscopy was applied as a novel tool for non-destructive, high-resolution discrimination of the two taxa. Intact fructus (<i>n</i> = 30 per species) were first screened macroscopically, then examined by stereo microscopy, optical microscopy, and 3D X-ray microscopy (0.3, 0.7, 1.5, 3.5, 18.06, 20.01 μm voxel size, Zeiss Xradia 520 Versa). The results showed that stereo microscopy, optical microscopy, and 3D X-ray microscopy collectively confirm the same conclusion from three distinct physical perspectives: surface topography, internal two-dimensional structure, and internal three-dimensional density distribution. The two Xanthium species differ significantly in burr spine morphology, fructus size and shape, the architecture and distribution of non-glandular and glandular trichomes, cotyledon conformation, and seed-coat cell patterning. In particular, 3D X-ray microscopy clearly resolves internal cotyledon spatial configurations and involucral cavity architectures, which furnishes critical endomorphic characters for taxonomic diagnosis. 3D X-ray microscopy provides unprecedented volumetric contrast of surface spines and internal seed architecture, permitting confident, non-destructive species identification. This study provides a basis for the safe clinical use of \u0000 <i>Xanthium sibiricum</i>\u0000 Patrin ex Widder. The frontier of 3D X-ray microscopy in plant systematics offers a novel, rapid, accurate and non-destructive protocol for the discrimination of morphologically elusive species.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"912-920"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"845-846"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148061871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Residual-Shuffle2DConv-Squeeze Network Approach for Enhanced Blood Cell Segmentation and Hematological Disorder Detection","authors":"Umarani P,&nbsp;Viswanathan P","doi":"10.1002/jemt.70119","DOIUrl":"10.1002/jemt.70119","url":null,"abstract":"<div>\u0000 \u0000 <p>Nowadays, blood cell segmentation has emerged as a popular solution for diagnosing hematological disorders. For hematological disorder detection, existing techniques face various limitations, including noise, weak edges, and intensity inhomogeneity. To rectify these problems, a novel Residual-Shuffle2DConv-Squeeze Network approach is proposed in this research to enhance the blood cell segmentation for hematological disorder diagnosis. Distinguishing blood cells, overlapped cells segmentation, edge detection, and morphological operations are the different stages of this approach for performing blood cell segmentation. The Residual-Shuffle Global Attention Network is used for morphological feature extraction, and this network integrates the Residual Network and Shuffle Global Attention Network. To retain fine-grained morphological features and capture variations of blood cell structure, the Residual-Shuffle Global Attention Network model is applied. The Shuffle Global Attention Network module has ShuffleNet and the Global Attention Mechanism. The ShuffleNet reduces the computational cost, and the Global Attention Mechanism helps to preserve crucial features through various layers. Finally, the 2DConv-SNN is applied to detect and classify hematological disorders. The comprehensive experiments are conducted on different datasets, including the Sickle Cell Disease Dataset and the Acute Lymphoblastic Leukemia dataset. The experimental results showcased that the Residual-Shuffle2DConv-Squeeze Network approach enhanced the detection of the hematological disorder with an accuracy of 98.69%, a dice coefficient of 97.05% and a Jaccard index of 96.43% respectively.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"862-883"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time Modulation of DNA Conductivity via Magnetic Fields Studied by C-AFM","authors":"Mingyan Gao,&nbsp;Jing Hu,&nbsp;Mengnan Liu,&nbsp;Bowei Wang,&nbsp;Junxi Wang,&nbsp;Xia Wang,&nbsp;Jie Jiao,&nbsp;Jingyu Wang,&nbsp;Junru Wang,&nbsp;Zuobin Wang","doi":"10.1002/jemt.70123","DOIUrl":"10.1002/jemt.70123","url":null,"abstract":"<div>\u0000 \u0000 <p>The charge transport properties of DNA make it a promising candidate for molecular electronics, yet the in situ dynamic regulation of its conductivity remains challenging. In this study, a conductive atomic force microscopy (C-AFM) system integrated with magnetic field modulation was developed to investigate the real-time conductivity of λ-DNA on Au-coated mica substrates. The results demonstrate that DNA molecular height positively correlates with tunneling current, with single double-stranded DNA (dsDNA) exhibiting a median current of −1.27 pA in the height range of 300 ~ 600 pm, while multi-stranded intertwined DNA fibers showed significantly enhanced conductivity (median current: −3.71 pA). Under a direct current (DC) magnetic field of 15 mT, both DNA forms exhibited suppressed conductivity, attributed to the Lorentz force effect. In contrast, an alternating current (AC) magnetic field (25 MHz) at 3 mT enhanced conductivity, whereas a 6 mT field led to a reduction. These findings provide direct experimental evidence for the real-time magnetic modulation of the transverse tunneling conductance through DNA molecules and offer a noncontact strategy for tuning DNA-based nanoelectronic devices.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"948-958"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HistoNeRF: An Accessible and Intelligent Approach for Comprehensive 2D-to-3D Histological Assessment","authors":"Kubilay Doğan Kiliç,&nbsp;Kaan Özyazici,&nbsp;Hüseyin Kisaoğlu,&nbsp;Zeynep Simge Yilmaz,&nbsp;Büşra Horuz,&nbsp;Ayşegül Taşkıran Özyazici","doi":"10.1002/jemt.70124","DOIUrl":"10.1002/jemt.70124","url":null,"abstract":"<div>\u0000 \u0000 <p>Histological analysis is central to biomedical research and diagnostic pathology, yet conventional two-dimensional (2D) sectioning captures only limited aspects of tissue architecture. Critical spatial relationships—such as tumor boundaries, stromal organization, and vascular networks—remain obscured, restricting diagnostic accuracy and biological interpretation. HistoNeRF addresses these limitations by adapting Neural Radiance Fields (NeRF) to reconstruct three-dimensional (3D) tissue volumes from routine histological sections. In this study, 84 toluidine blue (TB)–stained murine ovarian sections were digitized, alignment-corrected, and integrated into volumetric models. Tissue segmentation was performed using a convolutional neural network, while visualization was achieved through an interactive, GPU-accelerated interface. To ensure accessibility and reproducibility, a Python-based graphical application (HistoNeRF GUI) was developed following Human–Computer Interaction (HCI) principles and containerized with Docker, allowing installation-free deployment via Docker Hub. HistoNeRF produced high-fidelity 3D reconstructions (SSIM = 0.92; Dice similarity coefficient = 0.88), enabling expert histologists to better visualize follicular structures, stromal compartments, and vascular elements. The containerized GUI was deployed successfully from Docker Hub, providing immediate access to 3D reconstruction without a complex local setup. By overcoming the inherent constraints of 2D microscopy, HistoNeRF enhances the visualization, interpretability, and reproducibility of histological architecture. The HCI-guided, cross-platform interface supports scalability and rapid adoption in digital pathology workflows. Although validation was limited to murine ovarian tissue and one staining protocol, this framework can be extended across tissue types and clinical datasets. HistoNeRF bridges routine histology and 3D volumetric analysis through accurate, interactive reconstructions that advance diagnostic precision and biomedical research. While demonstrated on 84 serial TB-stained ovarian sections, broader validation across tissues, stains, and pathological conditions remains future work; to support this, we provide a Dockerized, modular pipeline for straightforward extension.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"921-931"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoscale Imaging of Neurons Under Near-Physiological Conditions Using Field-Emission Scanning Electron Microscopy","authors":"Yuri Yamada,&nbsp;Takaaki Hatanaka,&nbsp;Minoru Hirano","doi":"10.1002/jemt.70103","DOIUrl":"10.1002/jemt.70103","url":null,"abstract":"<div>\u0000 \u0000 <p>Visualization of neuronal ultrastructure facilitates molecular and biochemical analyses that may help to better elucidate neural function and information processing. While the neuron exists at the micron scale, critical events such as synaptic vesicle release and dendritic spine remodeling occur at the nanometer scale, necessitating submicron resolution. Scanning electron microscopy (SEM) provides high-resolution imaging at these scales. However, the commonly used dehydration-based sample preparation method induces morphological distortions, while environmental SEM requires specialized equipment that is costly and difficult to operate. The NanoSuit method has recently emerged as a promising alternative, enabling SEM observations under high-vacuum conditions without standard (dehydration-based) pretreatment. Although known to be successful when applied to specimens with protective surface layers such as insects, flowers, and wet tissues, its effectiveness when examining “bare” cultured cells has not been thoroughly explored. Here, we present a modified NanoSuit protocol for SEM examination of cultured neurons and compare it with standard pretreatment. We demonstrate that traditional methods frequently cause neuronal transection and loss of fine dendritic processes, particularly during early development of neurons. However, the modified NanoSuit approach preserves neuronal morphology, enabling clear visualization of thin neurites and their interactions. Further, we successfully implemented correlative light and electron microscopy (CLEM) using this method, enabling the colocalization of cytoskeletal proteins such as actin and tubulin with the surface features observed by SEM. This combination of morphological preservation and molecular localization provides a more accurate and holistic understanding of neuronal structures, benefiting studies on neural development, synaptic connectivity, and related biomedical applications.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"847-861"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Evaluation of MACSima and PhenoCycler-Fusion for Spatial Image Analysis in a Medulloblastoma Xenograft Model","authors":"Sokhoeun Heng,&nbsp;Taebok Lee,&nbsp;Seung Ah Choi,&nbsp;Haneul Lee,&nbsp;Seung-Ki Kim,&nbsp;Ji Hoon Phi","doi":"10.1002/jemt.70125","DOIUrl":"10.1002/jemt.70125","url":null,"abstract":"<div>\u0000 \u0000 <p>In spatial proteomics, multiplexed immunofluorescence (mIF) enables high-plex visualization of protein expression in preserved tissue, offering insights into tumor heterogeneity and the microenvironment. While MACSima and PhenoCycler-Fusion employ distinct strategies, direct comparisons under biologically controlled in vivo conditions remain limited. We applied both platforms to sagittal formalin-fixed, paraffin-embedded (FFPE) sections from an orthotopic xenograft mouse model of human medulloblastoma (MB), featuring leptomeningeal seeding (LMS). These longitudinal sections spanning brain and spinal cord allowed simultaneous assessment of areas with distinct cellular architecture. Fifteen-marker mIF was performed. MACSima utilized MICS technology with MACS iQ View for automated workflows; PhenoCycler-Fusion used a DNA-barcoded antibody system and QuPath for open-ended image processing. Segmentation was evaluated using identical MACSima data. Both platforms enabled high-plex imaging mIF while preserving tissue morphology. DAPI, Ki-67, and Actin were consistently detected across both systems. Ki-67 expression localized to densely packed tumor regions and was also observed in lower-density areas. Analyzing MACSima data, MACS iQ View detected fewer cells but a higher Ki-67 positive rate in dense regions; conversely, QuPath detected more cells but with a lower positivity rate. In low-density areas, both tools yielded similar results. These differences reflect distinct segmentation algorithms and thresholding strategies. This study confirms both platforms support mIF-based spatial proteomic analysis in complex, heterogeneous tissues. However, analysis tools influence quantification. Therefore, standardization of algorithmic settings and additional validation are crucial for precise data interpretation. This research provides practical insights for platform selection in basic, translational, and clinical applications by directly evaluating staining, image acquisition, and analysis pipelines.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"959-971"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microscopic Bioimaging Divulges 7-Ketocholesterol- Mediated Morphological Alterations, Inflammatory Responses and Dyshomeostasis in the Immune Reactions of Macrophages","authors":"Sangeetha Ravi,&nbsp;Livya Catherene Martin,&nbsp;Manikandan Kumaresan,&nbsp;Jaya Suriya Mani,&nbsp;Beulaja Manikandan,&nbsp;Manikandan Ramar","doi":"10.1002/jemt.70094","DOIUrl":"10.1002/jemt.70094","url":null,"abstract":"<div>\u0000 \u0000 <p>7-ketocholesterol (7KCh), a cytotoxic oxysterol derived from cholesterol oxidation, plays a pivotal role in the progression of lipid-driven inflammatory diseases such as atherosclerosis. Among its pathogenic mechanisms, 7KCh is known to trigger oxiapoptophagy, a unique convergence of oxidative stress, apoptosis and autophagy. While macrophages typically serve as key phagocytic cells for clearing lipid debris, exposure to 7KCh disrupts this homeostatic role, contributing instead to dysfunctional foam cell formation. Despite its clinical relevance, the precise morphological and mechanistic effects of 7KCh on macrophages remain insufficiently characterized. In this study, we investigated the impact of 7KCh on IC-21 macrophages using a comprehensive, multimodal imaging strategy that integrates phase contrast, differential interference contrast (DIC), fluorescence microscopy and quantitative confocal imaging. Exposure to increasing concentrations (2–10 μg/ml) of 7KCh for 24 h revealed distinct morphological alterations, including cytoplasmic vacuolization, disrupted cellular architecture and pronounced lipid accumulation. Oil red O staining confirmed the accumulation of neutral lipids, while functional assays demonstrated impaired pinocytosis and phagocytosis, hallmark features of foam cell transformation. These findings were further supported by molecular analysis indicating induction of pro-inflammatory markers and oxiapoptophagy signatures. Altogether, this work provides novel insight into the cytotoxic and inflammatory transformation of macrophages by 7KCh and highlights the power of advanced microscopy in delineating lipid-induced immune dysfunction. Our imaging-based approach offers a valuable platform for future investigations into oxysterol-mediated pathologies and therapeutic targeting of foam cell dynamics.</p>\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"89 6","pages":"884-900"},"PeriodicalIF":2.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}