Microscopy Research and Technique最新文献_第6页

SEM-Based Foliar Micromorphology for Taxonomic Delimitation of Selected Herbaceous Species. 基于sem的植物叶面显微形态学研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-11 DOI: 10.1002/jemt.70033

Iqra Qayyum, Mushtaq Ahmad, Aroosa Habib, Muhammad Zafar, Mohamed Fawzy Ramadan, Salman Majeed, Trobjon Makhkamov, Khislat Khaydarov, Shabir Ahmad, Muhammad Rizwan Khan

{"title":"SEM-Based Foliar Micromorphology for Taxonomic Delimitation of Selected Herbaceous Species.","authors":"Iqra Qayyum, Mushtaq Ahmad, Aroosa Habib, Muhammad Zafar, Mohamed Fawzy Ramadan, Salman Majeed, Trobjon Makhkamov, Khislat Khaydarov, Shabir Ahmad, Muhammad Rizwan Khan","doi":"10.1002/jemt.70033","DOIUrl":"https://doi.org/10.1002/jemt.70033","url":null,"abstract":"This study represents the first comprehensive effort to document the pollen taxonomy of plant species in the Hindukush-Karakoram-Himalaya region of northern Pakistan. The clarification of the leaf micromorphology of the selected herbaceous plants reveals characteristics like the stomata types, trichome types, and epidermal cell size and shape. The leaf micromorphology of 50 herbaceous plants revealed foliar anatomical traits, such as changes in epidermal cells, trichome morphology, and stomatal type. Stachys emodi has the longest epidermal cell length, measuring 69.54um at the adaxial and 66.65um at the abaxial surface. The largest guard cell length was recorded as 36.45 μm in Erigeron bonariensis, while the stomatal width measured 36.15 μm on the abaxial surface and 34.55 μm on the adaxial surface among the studied Asteraceous taxa. In anatomical features, diacytic, actinocytic, anomocytic, anisocytic, and paracytic stomata were studied. Anticlinal wall patterns were found to be undulating, straight, and sinuate. The epidermal cell shape was found to be polygonal, pentagonal, tetragonal, and irregular. The micromorphological epidermal anatomical traits have been recognized to serve as a foundation for the correct identification of herbaceous flora in the context of systematic relevance.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromorphological and Histochemical Study of Senna martiana, a Medicinal Herb 中药番泻的显微形态学和组织化学研究。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-11 DOI: 10.1002/jemt.70025

Antonio Cardoso da Silva Neto, Flávio Sousa Souto, Maria de Fátima Agra

{"title":"Micromorphological and Histochemical Study of Senna martiana, a Medicinal Herb","authors":"Antonio Cardoso da Silva Neto, Flávio Sousa Souto, Maria de Fátima Agra","doi":"10.1002/jemt.70025","DOIUrl":"10.1002/jemt.70025","url":null,"abstract":"<div>\u0000 \u0000 Senna martiana (Benth.) H.S. Irwin & Barneby is endemic to Brazil's dryland Caatinga domain. The species is known as “canafistula” in folk medicine, and its leaves are used as a laxative. Anatomical and histochemical studies of the species' vegetative organs (roots, stems, and leaves) were conducted to identify features that would support its taxonomic classification, as well as provide quality control for its ethnomedicinal use. Dried fragments from both leaflet surfaces were examined using scanning electron microscopy, and paradermal and transverse sections were used in histochemical tests to confirm the presence of lipids, starch, alkaloids, and nonstructural phenolic compounds. Senna martiana exhibits a striate cuticle on both leaflet surfaces, with straight to curved anticlinal cell walls on the adaxial face and sinuous walls on the abaxial face. The leaflets are amphistomatic, with a variety of stomatal types (paracytic, anisocytic, and anomotetracytic). The midrib is plane-convex with a collateral vascular system; the petiole is elliptic with 6–8 vascular bundles; the leaf rachis is ovate with 4–5 vascular bundles. Stems have a siphonostelic ectophloic vascular system. Idioblasts containing crystal sand, prismatic crystals, druses, and starch grains were observed in all vegetative organs. The contour and number of vascular bundles in the petiole and rachis, along with the sclerenchyma bundles enclosing the vascular system or roots, were distinctive features for Senna martiana. These characters constitute an important support for species identification, differentiation, and quality control of its ethnomedicinal drugs.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3083-3093"},"PeriodicalIF":2.1,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Microscopic Characterization and Antimicrobial Applications of Green-Synthesized Zinc Oxide Nanoparticles in Clinical Wound Healing Bandages. 绿色合成氧化锌纳米颗粒的显微表征及其在临床创面愈合绷带中的抗菌应用。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-11 DOI: 10.1002/jemt.70028

D Malathy, G Kaladevi, B Subash, A Sahaya Raja, Sandhanasamy Devanesan, Munusamy Settu, Saurav Dixit, V Prathipa

{"title":"Advanced Microscopic Characterization and Antimicrobial Applications of Green-Synthesized Zinc Oxide Nanoparticles in Clinical Wound Healing Bandages.","authors":"D Malathy, G Kaladevi, B Subash, A Sahaya Raja, Sandhanasamy Devanesan, Munusamy Settu, Saurav Dixit, V Prathipa","doi":"10.1002/jemt.70028","DOIUrl":"https://doi.org/10.1002/jemt.70028","url":null,"abstract":"The green synthesis of nanoparticles using plant extracts has garnered significant attention as a reliable and sustainable method for producing functional nanomaterials. Among these, zinc oxide (ZnO) nanoparticles are extensively studied for their potential biological applications. In this study, ZnO nanoparticles were synthesized through a green synthesis approach using an aqueous extract of Musa acuminata leaves and zinc acetate as precursors. The resulting nanoparticles were characterized using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction analysis (XRD), energy-dispersive x-ray analysis (EDX), and scanning electron microscopy (SEM). The UV-Vis spectra revealed characteristic absorption peaks around 350 nm, attributed to the nanoparticles' large excitation binding energy at room temperature. FTIR analyses confirmed the formation of zinc oxide chemical bonds, while XRD results indicated a hexagonal wurtzite crystal structure. SEM analysis showed that the nanoparticles had a nearly cuboid shape, and EDX analysis confirmed their high purity. The minimum inhibitory concentrations (MIC) of the synthesized ZnO nanoparticles were evaluated against Staphylococcus aureus and Escherichia coli cultures. Cotton wound bandages impregnated with ZnO nanoparticles at concentrations near the calculated MIC demonstrated significant antibacterial activity in vitro. These antimicrobial bandages show potential for use in treating and protecting infection-prone wounds, such as diabetic or burn-related injuries.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transfer Learning With Adam Gold Rush Optimization for Endometrial Disease Classification Using Histopathological Image 基于组织病理学图像的子宫内膜疾病分类的迁移学习优化。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-09 DOI: 10.1002/jemt.70027

Sudhagar Dhandapani, Ravikumar Subburam, Pretty Diana Cyril Cyriloose, Santhosh Kumar Balan

{"title":"Transfer Learning With Adam Gold Rush Optimization for Endometrial Disease Classification Using Histopathological Image","authors":"Sudhagar Dhandapani, Ravikumar Subburam, Pretty Diana Cyril Cyriloose, Santhosh Kumar Balan","doi":"10.1002/jemt.70027","DOIUrl":"10.1002/jemt.70027","url":null,"abstract":"<div>\u0000 \u0000 Uterine cancer also referred to as endometrial cancer, which significantly impacts the female reproductive organs. The early diagnosis increases the survival rates and also prevents the progression of endometrial cancer thereby, the novel Transfer Learning based Convolution Neural Network with Adam Gold Rush Optimization (TL-CNN_AdGRO) is proposed to classify endometrial cancer using histopathological images. The histopathological image is fed to the preprocessing phase, which uses an Adaptive Weighted Mean Filter (AWMF). Next, the segmentation of endometrial cancer is utilized by the Directional Connectivity Network (DConn-Net). Following segmentation, feature mining is carried out, which includes Local Boundary Summation Pattern (LBSP) and Local Gaber Binary Pattern Histogram Sequence Features (LGBPHS). Finally, the endometrial cancer classification is achieved using TL-CNN by employing hyperparameters from the Xception model. Here TL-CNN is trained by AdGRO algorithm, which is the combination of Adam Optimizer and Gold Rush Optimization. Compared to existing models, the proposed model achieves superior performance with an accuracy of 91.876%, a True Positive Rate (TPR) of 93.987%, and a True Negative Rate (TNR) of 89.876% for K-sample 8. The results confirm the effectiveness of TL-CNN_AdGRO, also it demonstrates strong performance, ensures robustness, improves the early detection of endometrial cancer, and making it a promising approach for histopathological image analysis.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3063-3082"},"PeriodicalIF":2.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Label-Free Nonlinear Optical Microscopy: Shedding Light on Oral and Dental Tissues 无标签非线性光学显微镜：在口腔和牙齿组织上发光。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-09 DOI: 10.1002/jemt.70012

Tarcília Aparecida Silva, José Alcides Almeida de Arruda, Nayara Conceição Marcos Santana, Renan Souza Cunha, Victor Zanetti Drumond, Leandro Malard Moreira, Bruno Augusto Benevenuto de Andrade, Juliane Isaac, Nicolas Dupré, Benjamin P. J. Fournier

{"title":"Label-Free Nonlinear Optical Microscopy: Shedding Light on Oral and Dental Tissues","authors":"Tarcília Aparecida Silva, José Alcides Almeida de Arruda, Nayara Conceição Marcos Santana, Renan Souza Cunha, Victor Zanetti Drumond, Leandro Malard Moreira, Bruno Augusto Benevenuto de Andrade, Juliane Isaac, Nicolas Dupré, Benjamin P. J. Fournier","doi":"10.1002/jemt.70012","DOIUrl":"10.1002/jemt.70012","url":null,"abstract":"Nonlinear optical microscopy (NLOM) enables 3D imaging with submicron resolution, and autofluorescence spectro-microscopy of oral and dental tissues shows promise for enhancing the analysis of both healthy and diseased tissues. We critically reviewed studies involving humans and animals, examining oral and dental tissues in vivo and ex vivo using label-free NLOM. A scientometric analysis was also conducted to assess the 25-year trajectory of NLOM in dentistry. A total of 78 studies from 17 countries were evaluated. Second harmonic generation (SHG) was the most commonly employed technique (83.3%), often combined with other NLOM methods (68%). The primary specimens imaged were the oral mucosa and teeth, with at least 70% of the studies contributing new morphological or biochemical insights and 34.6% generating data with potential clinical applications. SHG and cancer diagnosis were prominent descriptors, reflecting key findings from the most-cited articles. Data indicate that NLOM provides robust and reliable clinical information, particularly for cancer and dental caries diagnosis; however, challenges remain in translating this knowledge into practice. Integrating this technology into clinical devices could advance dentistry by creating noninvasive tools for long-term monitoring with minimal intervention and sample preparation.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3050-3062"},"PeriodicalIF":2.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144591701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy 使用光学投影断层扫描、数字薄层显微镜和磁共振显微镜的人体深筋膜三维显微结构表征。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-07 DOI: 10.1002/jemt.70035

Chiedozie Kenneth Ugwoke, Barbora Radochová, Jiří Janáček, Igor Serša, Patrik Ganc, Erika Cvetko, Nejc Umek

{"title":"3D Microstructural Characterization of Human Deep Fascia Using Optical Projection Tomography, Digital Light Sheet Microscopy, and Magnetic Resonance Microscopy","authors":"Chiedozie Kenneth Ugwoke, Barbora Radochová, Jiří Janáček, Igor Serša, Patrik Ganc, Erika Cvetko, Nejc Umek","doi":"10.1002/jemt.70035","DOIUrl":"10.1002/jemt.70035","url":null,"abstract":"Traditional histological methods provide limited insights into the complex 3D microstructure of fascia and its relationship to disease. This study explored the capacity of different 3D microscopy techniques for characterizing the microstructure of fascia lata (FL) and thoracolumbar fascia (TLF). Tissues from four donors were studied using optical projection tomography (OPT), digital light sheet (DLS) microscopy, and magnetic resonance microscopy (MRM). Samples for OPT and DLS were imaged with a custom OPT scanner and the DLS arm of a Leica Stellaris microscope, respectively. MRM was performed using a 9.4 T superconducting magnet and an NMR/MRI spectrometer. Reference histological evaluation was performed to guide the interpretation of 3D data. Image analyses were performed using FIJI and Ellipse software. DLS offered superior resolution, but all techniques revealed a trilaminar structure in both fasciae: a thick, collagen-rich intermediate layer flanked by thinner layers with loose connective tissue. The FL intermediate layer was thinner (210.5–258.7 μm) with longitudinally oriented collagen, while the TLF intermediate layer was thicker (302.3–343.6 μm) with both oblique and longitudinal fibers. The superficial layer in FL was thicker (128.8–161.5 μm) than in TLF (84.65–123.10 μm) across imaging modalities. The deep layer also varied between fasciae, with 54.3–73.8 μm in FL and 44.78–70.30 μm in TLF. Layer thickness measurements did not differ significantly across techniques. This study demonstrates the feasibility of different 3D microscopy techniques for visualizing and quantifying fascia extracellular matrix structure and organization, laying the groundwork for future investigations into potential structural alterations in disease.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3037-3049"},"PeriodicalIF":2.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/jemt.70035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lithium Disilicate Ceramics Pretreated With Ti: Sapphire fs, Er; Cr: YSGG, and Er: YAG Lasers: A Study on Mechanical Properties, Energy Dispersive Spectroscopy, Color Alterations, and Surface Topography Ti：蓝宝石fs， Er预处理二硅酸锂陶瓷Cr: YSGG和Er: YAG激光器：力学性能、能量色散光谱、颜色变化和表面形貌的研究。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-07 DOI: 10.1002/jemt.70032

Saud Ali Alqahtani, Ali Y. Alsaeed, Saeed M. Alqahtani, Shaheryar Shafqat, Muhammad Omar Niaz, Yusra Ishtiaque, Eisha Abrar

{"title":"Lithium Disilicate Ceramics Pretreated With Ti: Sapphire fs, Er; Cr: YSGG, and Er: YAG Lasers: A Study on Mechanical Properties, Energy Dispersive Spectroscopy, Color Alterations, and Surface Topography","authors":"Saud Ali Alqahtani, Ali Y. Alsaeed, Saeed M. Alqahtani, Shaheryar Shafqat, Muhammad Omar Niaz, Yusra Ishtiaque, Eisha Abrar","doi":"10.1002/jemt.70032","DOIUrl":"10.1002/jemt.70032","url":null,"abstract":"<div>\u0000 \u0000 To assess the efficiency of Er; Cr: YSGG laser (ECL), Er: YAG laser (EYL), and the Ti: sapphire femtosecond laser (TsFL) in terms of color change (ΔE) measured by a spectrophotometer, surface topography observed through SEM, surface roughness (Ra), and bond strength of Lithium disilicate glass–ceramic (LDC) to resin cement. A total of 84 LDC disks were manufactured and assigned randomly into four groups. Group 1-HFA treated, Group 2 treated with TsFL, Group 3 with EYL, and Group 4 with ECL. Each disk was coated with a silane coupling agent. Surface profiling of the LDC disc was evaluated via SEM and Ra was measured using a profilometer. The ΔE values were determined using the CIE L*a*b* system. Adhesive luting cement was applied to 10 samples from each group, followed by thermocycling to simulate aging. Shear bond strength (SBS) was assessed, and failure modes were inspected following SBS. ANOVA and Tukey's post hoc test were used for the comparison of means and SD among groups. The highest ΔE was observed in the control HFA (4.23 ± 1.02) treated group. Whereas, Group 2 TsFL (2.22 ± 1.11) pretreated samples displayed the lowest ΔE. Ra and SBS analysis unveiled that the maximum score of Ra (1155.01 ± 1.43 μm) and SBS (10.28 ± 0.17 MPa) was observed in Group 2 TsFL. Group 4 (ECL) conditioned discs showed the lowest Ra (1015.41 ± 1.31 μm) and bond integrity (8.15 ± 0.16 MPa). The TsFL serves as an effective alternative to traditional surface preparation methods as it enhances the mechanical properties of LDC (SBS and Ra) with no susceptible change in ΔE.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3029-3036"},"PeriodicalIF":2.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Different Concentrations of Bismuth Molybdate as an Electrocatalyst for Water Splitting and Antibacterial Activity 不同浓度钼酸铋电催化剂对水裂解及抗菌活性的影响。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-07 DOI: 10.1002/jemt.70022

Toaqeer Salman, Mohsin Javed, Sumera Zaib, Ahmad Alhujaily, Muhammad Adil Mansoor, Hamid Saeed Shah, Madiha Khan, Imtiaz Khan, Shahid Iqbal, Shaimaa A. M. Abdelmohsen, Randa A. Althobiti

{"title":"Effects of Different Concentrations of Bismuth Molybdate as an Electrocatalyst for Water Splitting and Antibacterial Activity","authors":"Toaqeer Salman, Mohsin Javed, Sumera Zaib, Ahmad Alhujaily, Muhammad Adil Mansoor, Hamid Saeed Shah, Madiha Khan, Imtiaz Khan, Shahid Iqbal, Shaimaa A. M. Abdelmohsen, Randa A. Althobiti","doi":"10.1002/jemt.70022","DOIUrl":"10.1002/jemt.70022","url":null,"abstract":"<div>\u0000 \u0000 In this work, five different molar concentrations of Bi2MoO6 are prepared using the hydrothermal method. SEM, XRD, and FTIR are used to find out the morphology of the synthesized materials as ratio 1 (1:1), 2 (0.8:1.2), 3 (1.2:0.8), 4 (0.6:1.4), and 5 (1.4:0.6). The newly formed nanomaterial is spherical to cubic in shape as per the SEM results, with some agglomerates. The water splitting and antibacterial activity of the electrocatalyst are carried out without any irradiation of light, which is the novelty of this research work. According to EIS (parameter of water splitting), the change transfer (Rct) value for ratio 3 (1.2:0.8) is 5 Ω and the Rct value for ratio 5 (1.4:0.6) is 8.48 Ω. The Rs value for ratio 1 (1:1) was very low, Rs = 5.77 Ω, compared to the Rs value for ratio 2 (0.8:1.2), Rs = 6.75 Ω. For instance, activity for ratio 1 (1:1) showed less overpotential for HER and OER, which led to more water splitting and a lower Rct number. For a better understanding, all the electrochemical factors and the time sequence of the different Bi2MoO6 ratios should be looked at: 1:1 ratio > 0.8:1.2 ratio > 1.2:0.8 ratio > 0.6:1.4 ratio > 1.4:0.6 ratio. The experimental results suggest that a 1:1 ratio between Bi and Mo exhibits higher electrochemical as well as antibacterial activity results.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3017-3028"},"PeriodicalIF":2.1,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pollen Morphology of Lauraceae Members From Garhwal Himalaya, (Uttarakhand, India) 印度北阿坎德邦Garhwal - himalayan樟科成员的花粉形态。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-05 DOI: 10.1002/jemt.70034

Shivani Negi, Ruchita Rawat, Prabhawati Tiwari

{"title":"Pollen Morphology of Lauraceae Members From Garhwal Himalaya, (Uttarakhand, India)","authors":"Shivani Negi, Ruchita Rawat, Prabhawati Tiwari","doi":"10.1002/jemt.70034","DOIUrl":"10.1002/jemt.70034","url":null,"abstract":"<div>\u0000 \u0000 The family Lauraceae, a basal angiospermic group within the Magnoliid clade, is important for understanding plant evolution and systematics. This study examines the pollen morphology of Lauraceae species from the Garhwal Himalaya, part of the Indian Himalayan Region (IHR), to explore their taxonomic and evolutionary significance. Pollen morphological features of 10 species of the family Lauraceae were examined and documented. Fresh flower buds were collected from the study area, and pollen grains were prepared using the standard acetolysis method. The morphological features of the pollen were examined using light microscopy (LM) and scanning electron microscopy (SEM). The pollen grains were observed to be monad, apolar, radially symmetrical, and inaperturate, with a nanogemmate exine surface bearing supratectal echinae (0.5–2 μm). Grain diameters ranged from 31.56 ± 2.56 μm (Neolitsea cuipala) to 43.25 ± 9.03 μm (Phoebe lanceolata). Comparative analysis revealed two major exine ornamentation patterns: (1) the Litsea type, with microechinae (~0.5 μm) and acute to obtuse tips, and (2) the Dodecadenia type, with larger echinae (1–2 μm) and acute tips. Cluster analysis supported these distinctions by grouping species based on exine features. The study confirms the stenopalynous nature and archaic pollen traits of the family. These findings provide a foundation for future research on phylogenetic relationships and ecological adaptations within the family. These findings strengthen the palynological characterization of Lauraceae and suggest that future comparative studies on both extant and fossil taxa of various basal angiosperm families may provide valuable insights into pollen evolution, adaptation, and phylogenetic relationships within Magnoliids.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"3009-3016"},"PeriodicalIF":2.1,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep Learning Automated Measurements of Expanded Polystyrene Beads Size Using Low-Resolution Micrography 深度学习自动测量膨胀聚苯乙烯珠尺寸使用低分辨率显微摄影。

IF 2.1 3区工程技术

Microscopy Research and Technique Pub Date : 2025-07-04 DOI: 10.1002/jemt.70019

Alejandro E. Rodríguez-Sánchez, Héctor Plascencia-Mora

{"title":"Deep Learning Automated Measurements of Expanded Polystyrene Beads Size Using Low-Resolution Micrography","authors":"Alejandro E. Rodríguez-Sánchez, Héctor Plascencia-Mora","doi":"10.1002/jemt.70019","DOIUrl":"10.1002/jemt.70019","url":null,"abstract":"<div>\u0000 \u0000 The analysis of microscopic characteristics of closed-cell polymeric foams, particularly bead size, is relevant for understanding properties such as thermal insulation, energy absorption, and compressive structural strength of these materials. This study presents an automated method based on Deep Learning models to measure the bead size of Expanded Polystyrene foams in low-resolution micrographs. The results of this approach were compared with manual measurements at two expanded polystyrene foam densities: 8.5 and 24 kg/m3. Hypothesis tests, including Student's t-test, Levene's test, and Mann–Whitney U test, were conducted and showed no significant differences between manual and automatic measurements. Student's t-test and Levene's test indicated that both methods have comparable means and variances, while the Two One-Sided Test confirmed that they were equivalent for bead size measurement. Additionally, the Mann–Whitney U test revealed no differences in medians, and Bland–Altman plot analyses demonstrated no systematic bias between the methods. Taken together, these results suggest that the proposed Deep Learning-based method is a reliable and precise substitute for the manual method in measuring the bead size of expanded polystyrene, making it suitable for practical use in the bead microstructural analysis of expanded polystyrene material.\u0000 </div>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":"88 11","pages":"2999-3008"},"PeriodicalIF":2.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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