Microscopy Research and Technique最新文献_第6页

Palynological Study of Some Astragalus L. Sections Distributed in Türkiye. 分布于台湾部分黄芪的孢粉学研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-08 DOI: 10.1002/jemt.24744

Halime Bilgi, Talip Çeter, Barış Bani, Serhat Karabicak, Oktay Biyiklioğlu, İbrahim Özkutlu, Laila Elfogohi

{"title":"Palynological Study of Some Astragalus L. Sections Distributed in Türkiye.","authors":"Halime Bilgi, Talip Çeter, Barış Bani, Serhat Karabicak, Oktay Biyiklioğlu, İbrahim Özkutlu, Laila Elfogohi","doi":"10.1002/jemt.24744","DOIUrl":"https://doi.org/10.1002/jemt.24744","url":null,"abstract":"In this study, the morphological properties of pollen of 14 species from 9 different sections of the genus Astragalus L. (Fabaceae) distributed in Türkiye were examined, identified, and compared under light microscope (LM) and scanning electron microscope (SEM). Pollen grains of the studied taxa are radially symmetrical and isopolar. Pollen shapes were determined as prolate, subprolate, spheroidal, and prolate-spheroidal. The polar axis lengths of the pollen grains were determined to be between 24.5 and 34.4 μm, and the equatorial axis lengths were determined to be between 22.4 and 27.5 μm. The aperture type of pollen grains is mainly trizonacolporate, rarely trisyncolporate. Colpus are thin and long with acute ends. The shapes of the pores were determined as oblate, suboblate, or oblate-spheroidal. Colpus lengths were found to be between 18.2 and 28.8 μm, and widths were found to be between 2.7 and 7.1 μm. In the studied species, four types of ornamentation were determined according to the differences in the polar region and equatorial region. Type 1: perforate in polar and equatorial regions; Type 2: perforate in polar region, microreticulate in equatorial region; Type 3: microreticulate in polar and equatorial regions; Type 4: psilate-perforate in polar region, microreticulate in equatorial region. Principal component analysis (PCA) and unweighted pair group method using arithmetic average (UPGMA) analyses showed that morphological characters of pollen grains were effective in distinguishing species but could not contribute significantly to a distinction according to sections. Pollen size, colpus characteristics, and ornamentation were determined to be important characteristics that distinguish the studied taxa. This study contributes to Astragalus taxonomy and different sub-branches of palynology.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Toward Efficient Hydrogen Generation Using Nickel-Molybdenum Catalyst and Its Environmental Sustainability. 镍钼催化剂高效制氢及其环境可持续性研究。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-05 DOI: 10.1002/jemt.24762

Sadaf Sarfraz, Nasir Khan, Manzar Zahra, Khizar Hussain Shah, Mohsin Javed, Ammar Zidan, Ali Bahadur, Shahid Iqbal, Sajid Mahmood, Abd-ElAziem Farouk, Salman Aloufi

{"title":"Toward Efficient Hydrogen Generation Using Nickel-Molybdenum Catalyst and Its Environmental Sustainability.","authors":"Sadaf Sarfraz, Nasir Khan, Manzar Zahra, Khizar Hussain Shah, Mohsin Javed, Ammar Zidan, Ali Bahadur, Shahid Iqbal, Sajid Mahmood, Abd-ElAziem Farouk, Salman Aloufi","doi":"10.1002/jemt.24762","DOIUrl":"https://doi.org/10.1002/jemt.24762","url":null,"abstract":"In this study, a catalyst with Ni-Mo combination was synthesized using the electric heating/reductive tempering method. Nickel (II) nitrate hexahydrate and ammonium molybdate were combined in a ratio of 1.1 in this approach. The mixture was milled into a fine powder. It was heated to 950°C to 1000°C in a seething hood. The disappearance of green shading and emission of brownish-yellow fumes indicated that the reaction was completed. XRD has been used to determine the crystallinity of the combined Ni-Mo amalgam, SEM was used to investigate the surface morphology of the orchestrated Ni-Mo compound, and inductively coupled plasma examinations were carried out to evaluate elemental percentage (%) of the integrated sample of Ni-Mo combination. In addition, an electrical impedance analysis of as-synthesized Ni-Mo alloy was conducted to estimate hydrogen production in an electrochemical reaction. The electrical impedance results indicate that the synthesized Ni-Mo catalyst exhibited an efficient charge-transfer kinetics with a low charge-transfer resistance (5.35 Ω). The onset potential value achieved was 18 mV with overpotential of -100 mV in IM KOH, possessing a turnover frequency of 0.91H2 s-1. These findings underscore Ni-Mo catalyst as a promising catalyst for hydrogen generation studies. The results of this study are anticipated to be of potential significant importance in providing a cost-effective approach towards the synthesis of Ni-bimetallic catalyst, which in the future can serve as a promising candidate for applications involving sustainable hydrogen generation. Additionally, the proposed method's study of its greenness using the Analytical Greenness Calculator (AGREE) can help advance the usage of renewable and environmentally friendly energy sources.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142786237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphoanatomy and Histochemistry of Symplocos tetrandra (Mart.) Mart. (Symplocaceae). 四分香的形态解剖学和组织化学研究集市。(Symplocaceae)。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-05 DOI: 10.1002/jemt.24745

Juliana de Fátima Garcia, Lorene Armstrong, Leandro Taborda da Rocha, Cíntia Aparecida Dos Anjos, Karine Amorim Fladzinski, Marilis Dallarmi Miguel, Obdulio Gomes Miguel, Deise Prehs Montrucchio, Josiane de Fátima Gaspari Dias

{"title":"Morphoanatomy and Histochemistry of Symplocos tetrandra (Mart.) Mart. (Symplocaceae).","authors":"Juliana de Fátima Garcia, Lorene Armstrong, Leandro Taborda da Rocha, Cíntia Aparecida Dos Anjos, Karine Amorim Fladzinski, Marilis Dallarmi Miguel, Obdulio Gomes Miguel, Deise Prehs Montrucchio, Josiane de Fátima Gaspari Dias","doi":"10.1002/jemt.24745","DOIUrl":"https://doi.org/10.1002/jemt.24745","url":null,"abstract":"The Symplocaceae family comprises 406 species, all of which are classified within a single genus, Symplocos Jacq., which is distributed across multiple continents. In Brazil, this genus is predominantly found in the Atlantic Forest and Cerrado regions and is characterized by the presence of secondary metabolites, such as iridoids, terpenoids, and saponins with biological activities. Moreover, the literature research has unveiled the potential of this genus for antimicrobial, antitumor, and anti-HIV applications. This research conducted morphoanatomical, histochemical studies using light microscopy and scanning electron microscopy (SEM) analysis of the leaves and stems of Symplocos tetrandra (Mart.) Mart. Cross-sections and paradermal were performed. The investigation revealed the presence of prismatic calcium oxalate crystal in petiole and druses calcium oxalate crystals, in petiole and leaf blade. Paracytic stomata on the abaxial surface, lignified pith in the stem, which was detected with the phloroglucinol staining. Oil bodies, uniseriate epidermis, and a thin lipidic cuticle were confirmed with Sudan III. These findings suggest potential anatomical markers for taxonomic classification of the species. The data obtained in this study contributes for species identification, and the presence of metabolites can guide future pharmacological investigation with S. tetrandra.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142780436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Russula iqbalii sp. nov., Identified in R. Subsect. Maculatinae from Pakistan, Based on Morphology, Microscopy, and Phylogeny. Russula iqbalii sp. nov.，鉴定于R.亚科。基于形态学、显微学和系统发育的巴基斯坦斑虫科。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-05 DOI: 10.1002/jemt.24761

Muhammad Naveed, Sana Jabeen, Hira Ijaz, Memoona Azeem, Mehboobullah Khan, Sadiq Ullah

引用次数: 0

The Effect of Immediate Dentin Sealing Using Different Universal Adhesives on the Bond Strength of Pretreated Monolithic Zirconia to Dentin and Microscopic Morphological Alterations. 不同通用胶粘剂直接封闭牙本质对预处理整体氧化锆与牙本质结合强度及微观形态改变的影响。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-02 DOI: 10.1002/jemt.24730

Leyla Fazlioglu, Burcu Oglakci Ozkoc, Dilek Tagtekin

{"title":"The Effect of Immediate Dentin Sealing Using Different Universal Adhesives on the Bond Strength of Pretreated Monolithic Zirconia to Dentin and Microscopic Morphological Alterations.","authors":"Leyla Fazlioglu, Burcu Oglakci Ozkoc, Dilek Tagtekin","doi":"10.1002/jemt.24730","DOIUrl":"https://doi.org/10.1002/jemt.24730","url":null,"abstract":"This study evaluated the effects of immediate dentin sealing (IDS) using different universal adhesives on microtensile bond strength (μTBS) of pretreated monolithic translucent tetragonal polycrystalline zirconia-based CAD/CAM restoration to dentin and microscopic morphological alterations. Mid-coronal dentin were obtained from 32 human molars and randomly allocated into 8 groups according to the presence/absence of IDS, universal adhesives for IDS (Single Bond Universal/SBU, Clearfil Quick Bond/CUQ, Optibond Universal/OBU) and zirconia surface pretreatments (tribochemical silica coating-30 μm CoJet particles/TSC, sandblasting-50 μm Al2O3/SB): Group TSC, Group TSC+SBU, Group TSC+CUQ, Group TSC+OBU, Group SB, Group SB+SBU, Group SB+CUQ, Group SB+OBU. CAD/CAM restorations (Lava Plus High Translucency Zirconia Disc) were produced, luted, and subjected to 10.000 thermocycles (5°C-55°C). Zirconia-dentin bars (2 × 2 × 8 mm) were subjected to μTBS test (n = 16). Surface morphology of zirconia and zirconia-dentin interfaces were analyzed under SEM (n = 1). Two-way ANOVA and Bonferroni tests were used for statistical analyses (p < 0.05). Regarding the presence of IDS, Group TSC + OBU showed statistically higher μTBS than Group TSC (p < 0.05). Comparing the IDS applied groups, Group TSC+OBU and Group SB+OBU showed statistically higher μTBS than Group TSC+CUQ and Group SB+CUQ, respectively (p < 0.05). According to the surface pretreatments, no significant differences in μTBS were found (p > 0.05). A thick, intact hybrid layer with long resin tags were observed for Group TSC+OBU and Group SB+OBU. After tribochemical silica coating or sandblasting pretreatments of monolithic zirconia, IDS procedure with Optibond Universal could affect the bond strength to dentin and morphological appearance, positively.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Multi Scale Attention Network Optimized With Archerfish Hunting Optimization Algorithm for Diabetics Prediction. 基于射水鱼狩猎优化算法的双多尺度注意网络糖尿病预测。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-02 DOI: 10.1002/jemt.24739

Helina Rajini Suresh, K Anita Davamani, Hemalatha Chandrasekaran, N Prabu Sankar

{"title":"Dual Multi Scale Attention Network Optimized With Archerfish Hunting Optimization Algorithm for Diabetics Prediction.","authors":"Helina Rajini Suresh, K Anita Davamani, Hemalatha Chandrasekaran, N Prabu Sankar","doi":"10.1002/jemt.24739","DOIUrl":"https://doi.org/10.1002/jemt.24739","url":null,"abstract":"Diabetes is a chronic disease that occurs when the body cannot regulate blood sugar levels. Nowadays, the screening tests for diabetes are developed using multivariate regression methods. An increasing amount of data is automatically collected to provide an opportunity for creating challenging and accurate prediction modes that are updated constantly with the help of machine learning techniques. In this manuscript, a Dual Multi Scale Attention Network optimized with Archerfish Hunting Optimization Algorithm is proposed for Diabetes Prediction (DMSAN-AHO-DP). Here, the data is gathered through PIMA Indian Diabetes Dataset (PIDD). The collected data is fed towards the preprocessing to remove the noise of input data and improves the data quality by using Contrast Limited Adaptive Histogram Equalization Filtering (CLAHEF) method. Then the preprocessed data are fed to Multi-Level Haar Wavelet Features Fusion Network (MHWFFN) based feature extraction. Then the extracted data is supplied to the Dual Multi Scale Attention Network (DMSAN) for diabetic or non-diabetic classification. The hyper parameter of Dual Multi Scale Attention Network is tuned with Archerfish Hunting Optimization (AHO) algorithm, which classifies diabetic or non-diabetic accurately. The proposed DMSAN-AHO-DP technique is implemented in Python. The efficacy of the DMSAN-AHO-DP approach is examined with some metrics, like Accuracy, F-scores, Sensitivity, Specificity, Precision, Recall, Computational time. The DMSAN-AHO-DP technique achieves 23.52%, 36.12%, 31.12% higher accuracy and 16.05%, 21.14%, 31.02% lesser error rate compared with existing models: Enhanced Deep Neural Network based Model for Diabetes Prediction (EDNN-DP), Indian PIMA Dataset using Deep Learning for Diabetes Prediction (ANN-DP), and Enhanced Support Vector Machine with Deep Neural Network Learning strategies for Diabetes Prediction (SVM-DNN-DP).","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetic and Isothermal Analysis of the Adsorptive Elimination of Direct Yellow 26 Dye Utilizing Activated Bioadsorbent From Textile Effluent. 活性生物吸附剂吸附去除纺织废水中直接黄26染料的动力学及等温分析。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-02 DOI: 10.1002/jemt.24760

Mirza Nadeem Ahmad, Sohail Nadeem, Mohsin Javed, Ammar Zidan, Muhammad Naveed Anjum, Muhammad Fayyaz Farid, Ali Bahadur, Shahid Iqbal, Sajid Mahmood, Abd-ElAziem Farouk, Salman Aloufi

{"title":"Kinetic and Isothermal Analysis of the Adsorptive Elimination of Direct Yellow 26 Dye Utilizing Activated Bioadsorbent From Textile Effluent.","authors":"Mirza Nadeem Ahmad, Sohail Nadeem, Mohsin Javed, Ammar Zidan, Muhammad Naveed Anjum, Muhammad Fayyaz Farid, Ali Bahadur, Shahid Iqbal, Sajid Mahmood, Abd-ElAziem Farouk, Salman Aloufi","doi":"10.1002/jemt.24760","DOIUrl":"https://doi.org/10.1002/jemt.24760","url":null,"abstract":"Due to their widespread usage in recent years, synthetic dyes may be difficult to remove and pose a health concern. Bioadsorbents proved a low-cost and sustainable method for dye removal. In this study, straight yellow 26 is extracted from textile effluent using sugarcane bagasse. Sugarcane bagasse was treated with propionic acid to enhance the adsorption capability and 0.25 mm particle size was used for further studies which was confirmed by BET analysis. Standard solutions of direct yellow 26 dye were prepared from 10 to 100 ppm concentrations and absorbance was recorded with the help of a UV visible spectrophotometer. After optimizing different parameters (concentration of dye and bioadsorbent dose, pH, time, and particle size), the studies explored that the maximum dye removal percentage was 89% obtained at pH 3, contact time 120 min, particle size 0.25 mm, high adsorbent, and low concentration of dye solution. The kinetic studies were also employed to comprehend the adsorption isotherm and Freundlich isotherm that revealed the pseudo-first-order adsorption process.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Band Gap Engineering of Binary and Cobalt-Doped PbS Thin Films Integrated by SILAR Method for Optoelectronic Potentials. 用SILAR方法集成二元和钴掺杂PbS薄膜的光电势带隙工程。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-12-02 DOI: 10.1002/jemt.24750

Tanzeela Fazal, Sajid Mahmood, Bushra Ismail, Mazloom Shah, Ammar Zidan, Ali Bahadur, Shahid Iqbal, Komal Rizwan, Shahid Alam, Fayyaz Ali, Abd-ElAziem Farouk, Salman Aloufi

{"title":"Band Gap Engineering of Binary and Cobalt-Doped PbS Thin Films Integrated by SILAR Method for Optoelectronic Potentials.","authors":"Tanzeela Fazal, Sajid Mahmood, Bushra Ismail, Mazloom Shah, Ammar Zidan, Ali Bahadur, Shahid Iqbal, Komal Rizwan, Shahid Alam, Fayyaz Ali, Abd-ElAziem Farouk, Salman Aloufi","doi":"10.1002/jemt.24750","DOIUrl":"https://doi.org/10.1002/jemt.24750","url":null,"abstract":"Thin films of PbS, both undoped and cobalt-doped (Co-PbS), were produced on glass substrates using the straightforward and controllable approach of sequential ionic layer adsorption and reaction (SILAR). The reactive substances employed to deposit the PbS thin films were lead nitrate (Pb(NO3)2), cobalt nitrate (Co(NO₃)₂(H₂O)₆), sodium thiosulphate (Na2S2O3) and H2O for different dipping concentrations of lead and cobalt. The films were adherent to the substrate and were compact, and crack-free with a shiny silver color. The films were structurally characterized by XRD and were found well crystallized according to the face-centered cubic formation. The films were optically characterized by the UV-Vis. spectrophotometer and absorption were found stronger in the UV and UV-Vis. region and then diminishes. Band gaps were determined to be between 1.8 eV and 2.2 eV, making them significant materials with the option of band gap engineering according to the desire by manipulating the compositions.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalizing Muscle Regeneration: Cocoa Polyphenols Shield Mitochondrial Integrity and Boost Myogenesis Under Oxidative Stress. 振兴肌肉再生：可可多酚保护线粒体完整性，促进氧化应激下的肌肉生成。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-11-30 DOI: 10.1002/jemt.24755

Jose Angel Garcia-Merino, Vittoria Carrabs, Fabio Ferrini, Sara Salucci, Michela Battistelli, Sabrina Burattini, Francesca Luchetti, Maria Gemma Nasoni, Giosuè Annibalini, Matteo Micucci, Federico Gianfanti, Piero Sestili, Mar Larrosa, Elena Barbieri

{"title":"Revitalizing Muscle Regeneration: Cocoa Polyphenols Shield Mitochondrial Integrity and Boost Myogenesis Under Oxidative Stress.","authors":"Jose Angel Garcia-Merino, Vittoria Carrabs, Fabio Ferrini, Sara Salucci, Michela Battistelli, Sabrina Burattini, Francesca Luchetti, Maria Gemma Nasoni, Giosuè Annibalini, Matteo Micucci, Federico Gianfanti, Piero Sestili, Mar Larrosa, Elena Barbieri","doi":"10.1002/jemt.24755","DOIUrl":"https://doi.org/10.1002/jemt.24755","url":null,"abstract":"In this study, we describe the effect of cocoa polyphenol extract (CPE, from flavanols-rich cocoa) on myogenic differentiation in murine myoblasts (C2C12 cells) exposed to H2O2. The myogenic program was monitored using morphological, ultrastructural, and molecular approaches. Treatment with 100 μM of H2O2 for 1 h decreased cell viability. C2C12 (D1) exposed to H2O2 shows more apoptotic and necrotic cells, and mitochondria appear emptied, with cristae heavily damaged. To evaluate the effect of CPE on myoblast viability and myotube formation, 10 μg/mL of CPE were added 24 h prior to H2O2 treatment and cells were supplemented with fresh CPE every 24 h during differentiation. Supplementation with CPE protected C2C12 myoblasts from H2O2-induced oxidative damage both at early (D1) and late (D6) phases of differentiation, preventing cell death and mitochondrial damage. The number of mitochondria (per area of cell surface) increased 2-fold in both control and in CPE-supplemented and mitochondria in myotubes D6 showed a greater extension of mitochondrial cristae than mitochondria in D1. At D1 and D6 the monolayers showed surface and inner cell features relatively comparable to the untreated control suggesting that CPE supplementation significantly mitigated the effect of H2O2. Preliminary data obtained by the myogenic index (Giemsa staining) suggested that CPE-supplemented cells were partially protected from H2O2-induced myogenesis inhibition. The CPE supplementation seems to preserve the mitochondrial integrity and the myogenic differentiation ability of oxidatively injured C2C12 ensuing further nutraceutical perspectives.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cefixime-Infused Green ZnO Nanoplatelets With Enhanced Biological Potential. 头孢昔肟注入的绿色ZnO纳米血小板具有增强的生物潜能。

IF 2 3区工程技术

Microscopy Research and Technique Pub Date : 2024-11-30 DOI: 10.1002/jemt.24719

Amna Munsaf, Muhammad Naeem Ahmed, Ihsan Ul Haq, Bilal Akram, Mahmoud A A Ibrahim

{"title":"Cefixime-Infused Green ZnO Nanoplatelets With Enhanced Biological Potential.","authors":"Amna Munsaf, Muhammad Naeem Ahmed, Ihsan Ul Haq, Bilal Akram, Mahmoud A A Ibrahim","doi":"10.1002/jemt.24719","DOIUrl":"https://doi.org/10.1002/jemt.24719","url":null,"abstract":"The escalating resistance to traditional antibiotics causes a significant hazard to public health, demanding innovative antimicrobial strategies. This study introduces cefixime-infused green-synthesized zinc oxide nanoplatelets (ZnO NPts) highlighting their enhanced biological potential. The successful formation of ZnO NPts and their subsequent infusion with cefixime were confirmed using various characterization techniques: UV-visible spectroscopy, Fourier transform infrared spectroscopy, x-ray diffraction, scanning electron microscopy, transmission electron microscopy, energy dispersive spectroscopy, and dynamic light scattering. Comparing cefixime-functionalized ZnO NPts with pure ZnO and cefixime alone, biological assessments revealed that the former exhibited stronger antifungal activity against the tested strains. Moreover, these NPts demonstrated the highest cytotoxicity in tests with Artemia salina larvae and pronounced antioxidant activity in TAC, TRP, and DPPH assays. These findings emphasize the significant potential of cefixime-infused ZnO NPts for various biomedical applications, offering enhanced antifungal, cytotoxic, and antioxidant properties.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":" ","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0