Microscopy Research and Technique最新文献

{"title":"RETRACTION: Light and Scanning Electron Microscopy‐based Foliar Morpho‐Anatomical Comparison of Selected Family Rosaceae Members Distributed in District Lahore, Punjab, Pakistan","authors":"","doi":"10.1002/jemt.24694","DOIUrl":"https://doi.org/10.1002/jemt.24694","url":null,"abstract":"RETRACTION: S. Shaheen, J. Sharifi‐Rad, M. Ali, Z. Shamim, B. Rasool, M. Ashfaq, H. Mukhtar, N. Harun, U. Hanif and R. Siddique, “Light and Scanning Electron Microscopy‐based Foliar Morpho‐Anatomical Comparison of Selected Family Rosaceae Members Distributed in District Lahore, Punjab, Pakistan,” <jats:italic>Microscopy Research and Technique</jats:italic> 85, no. 4 (2022): 1597–1610, <jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"https://doi.org/10.1002/jemt.24021\">https://doi.org/10.1002/jemt.24021</jats:ext-link>.The above article, published online on 09 December 2021 in Wiley Online Library (<jats:ext-link xmlns:xlink=\"http://www.w3.org/1999/xlink\" xlink:href=\"http://wileyonlinelibrary.com\">wileyonlinelibrary.com</jats:ext-link>), has been retracted by agreement between the journal Editor‐in‐Chief, Alberto Diaspro; and Wiley Periodicals LLC. The retraction has been agreed due to duplications observed within the image presented in Figure 1r. Further duplications were also observed between Figures 2d and 2q, 2h and 2r and between 2i and 2s. The authors admit that they made mistakes during the preparation of the figures and provided some original data. However, due to the nature and extent of the errors, the editors have lost confidence in the results and conclusions of this study.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142175322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved protocol for histological and histopathological preparation of large eyes.","authors":"Mingliang Zhang, Raquel Lima E Silva, Lingli Zhou, Sean F Hackett, Peter A Campochiaro, Jikui Shen","doi":"10.1002/jemt.24698","DOIUrl":"https://doi.org/10.1002/jemt.24698","url":null,"abstract":"<p><p>The development of new treatments for ocular diseases often requires investigating eyes similar in size and structure to human eyes. Such studies are challenging because analyzing the histopathology of large, human-sized eyes can be technically difficult. In particular, obtaining high-quality frozen sections is almost impossible due to the formation of ice crystals in the vitreous, which causes crush artifacts during the procedures of section and post sectioning manipulations. Herein, we describe a new method that provides high-quality frozen sections for large eyes and demonstrate its usefulness in the eyes of rabbits, pigs, minipigs, monkeys, and humans. We observed that artifactual separation of the photoreceptors from the retinal pigment epithelium is minimized and photoreceptor morphology is preserved. This method can be highly beneficial for investigators seeking to translate new treatments for ocular disease into the clinic. RESEARCH HIGHLIGHTS: Histopathological analysis of large and human-sized eyes presents significant challenges, particularly in obtaining high-quality frozen sections. A multistep fixation followed by vitreous removal and replacement ensures better cryopreservation and embedding of large eyes, minimizing the morphological and structural retinal loss found in many studies. Our results demonstrate that a multistep fixation and cryopreservation method for large eyes in histopathology consistently minimizes the artifactual separation of photoreceptors from the retinal pigment epithelium, thereby preserving photoreceptor morphology and providing high-quality frozen sections. A new method providing high-quality sections is necessary and will be highly useful for investigators aiming to translate new treatments for ocular diseases into clinical applications.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological potential of Argyrolobium roseum (Camb.) Jaub & Spach mediated silver nanoparticles and their effect on the growth of wheat seeds.","authors":"Arooj Muneer, Wasim Akhtar, Memoona Samad, Sadia Zafar, Iram Fatima, Syed Hussain Imam Abidi, Razia Kalsoom, Amir Shahbaz","doi":"10.1002/jemt.24695","DOIUrl":"https://doi.org/10.1002/jemt.24695","url":null,"abstract":"<p><p>The green synthesis of silver nanoparticles (AgNPs) using plant-based derivatives is getting attention for biological applications because of their small dimensions and shape. In this study, AgNPs were prepared using leaf extract of Argyrolobium roseum (A. roseum) (Camb.) Jaub. & Spach. and then characterized via Fourier transform infrared spectroscopy (FTIR), x-ray diffraction (XRD), scanning electron microscopy (SEM) and ultraviolet-visible absorption (UV-Vis) spectroscopy. The UV-visible spectrum displayed a absorption peak at 450 nm and x-ray diffraction depicted a crystalline nature of biogenic NPs. FTIR analysis showed various functional groups involved in the reduction and capping of AgNPs while SEM revealed the spherical form of synthesized AgNPs. The antibacterial assay was conducted using disc diffusion assay and highest inhibition zones were recorded against Bacillus subtilis (B. subtilis) (9.6 ± 0.5 mm) and Staphylococcus aureus (S. aureus) (8.6 ± 0.5 mm). The antioxidant potential was assessed via DPPH scavenging assay and highest percentage inhibition (89%) was observed at 100 μg/mL. Subsequently, different concentrations of A. roseum AgNPs were applied on the wheat seedlings to investigate its effects on different growth parameters. After applying AgNPs, significant increase in the fresh weight (FW), dry weight (DW), root length (RL), shoot length (SL), leaf number (LN) and chlorophyll content (CC) in wheat (Akbar-2019 variety) seedlings was observed in comparison to the control seedlings. Overall, A. roseum mediated synthesis of AgNPs was cost-effective and safe and can be used in agriculture, biomedical and other fields. RESEARCH HIGHLIGHTS: Synthesis and characterization of A. roseum AgNPs was done. Biogenic AgNPs revealed potent antibacterial and antioxidant potential. A. roseum mediated AgNPs also increases the growth and germination of wheat seedlings.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The optical properties of nano-structural α-Fe₂O₃ dependence on the shape.","authors":"Yousheng Liu, Qinnan He, Zhan Wang, Xiaomei Jiao, Yinping Zhang","doi":"10.1002/jemt.24686","DOIUrl":"https://doi.org/10.1002/jemt.24686","url":null,"abstract":"<p><p>Three different crystal morphologies of α-Fe₂O₃, including uniform hexagonal, square, and rhombic shapes, were prepared according to the aqueous-thermal reaction. The hexagonal-shaped α-Fe₂O₃ was enclosed by the 104 plane, while the square and rhombic structures were enclosed by the 110 plane. Two absorption peaks at 455 and 532 cm^-1 were found for the perpendicular (⊥) modes, and one absorption peak at 650 cm^-1 appeared for the parallel (||) mode for hexagon-shaped α-Fe₂O₃ during analysis by Fourier-transform infrared spectroscopy. However, the peaks of square- and rhombic-shaped α-Fe₂O₃ for perpendicular (⊥) mode blueshifted, and the former two peaks merged together forming a broad band at approximately 480 cm^-1. For Raman spectra determination, the peaks arose from the Brillouin zone center, and two additional peaks were observed at 660 and 1320 cm^-1, belonging to 1 longitudinal optical (1LO) and 2 longitudinal optical (2LO) modes. All three materials exhibited higher intensities when excited at a wavelength of 633 cm^-1. Furthermore, in the polarization state, the centers of all peak positions slightly shifted for hexagon-shaped α-Fe₂O₃, but all peak positions for square-shaped and rhombic-shaped α-Fe₂O₃ exhibited a significant blueshift. The structure of hexagon-shaped α-Fe₂O₃ was relatively tolerant regarding the polarization properties of vibration modes; however, the symmetry of crystal square-shaped and rhombic-shaped α-Fe₂O₃ changed, subsequently revealing different optical properties. RESEARCH HIGHLIGHTS: The hexagon-shaped, square-shaped, and rhombic-shaped α-Fe₂O₃ enclosed by different planes were synthesized. The Fourier Transform Infrared spectrometer peaks of α-Fe₂O₃ depended on their hexagon, square and rhombic shapes. Compared with hexagon-shaped α-Fe₂O₃, the Raman peaks for square and rhombi ones significantly shifted. The hexagon-shaped α-Fe₂O₃ is relatively tolerant regarding the polarization properties.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The abundance and analytical characterization of microplastics in the surface water of Haryana, India.","authors":"Nishita Narwal, Deeksha Katyal","doi":"10.1002/jemt.24657","DOIUrl":"https://doi.org/10.1002/jemt.24657","url":null,"abstract":"<p><p>Microplastic (MP) contamination has become a serious environmental concern that affects terrestrial environments, aquatic ecosystems, and human health. The current study assesses the presence, abundance, and morphology of MPs present in the surface water of Rohtak district, Haryana, India, which is rapidly undergoing industrialization. While the morphological studies of MPs were conducted through stereo microscopy and field emission-scanning electron microscopy (FE-SEM), the elemental composition of polymers was analyzed through attenuated total reflectance-Fourier transform infrared (ATR-FTIR). The results revealed that the surface water was significantly contaminated by polyethylene, polypropylene, and polystyrene. Moreover, the abundance of MPs was found to be 16-28 particles/L with an average value of 23 particles/L. Most of the MPs had fibrous morphology with the specifics being, fibers (43.9%), fragments (23.7%), films (17%), and pellets (15.4%). The MPs exhibited a size range of 0.61-4.87 mm, with an average size measured at 2.03 ± 0.04 mm. Also, the MP pollution load index values for the surface water bodies were found to be below 10, indicating a low risk category. Though currently designated as \"low risk,\" it is important that mitigation strategies be brought over at this juncture to further prevent the deterioration of quality of water. Thus, this study not only intends to bring forth the impact of human activities, industrial waste, open waste dumping, and inadequate municipal waste management practices on increasing MP concentration but also highlights the sustainable alternatives and strategies to address this emerging pollutant in urban water systems. For further prevention, the implementation of stringent regulations and on-site plastic waste segregation is a critical component in preventing the disposal of plastic waste in surface water bodies. RESEARCH HIGHLIGHTS: The abundance of MPs was found to be 16-28 particles/L, with an average value of 23 particles/L. The surface water bodies in Rohtak district fall into the hazard categories of low risk with values less than 10. The overall MP concentration in water, across all five areas, based on color was in order: white/transparent (39.1%), black (15%), gray (9.1%), green (8.7%), blue (7.8%), red (7.8%), orange (6.3%), and yellow (6.1%). The dominant polymers were polyethylene (PE) (42%) and polypropylene (41%) as determined by FTIR spectroscopy.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142120247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fighting sepsis-induced liver damage with biosynthesized silver nanoparticles.","authors":"Amal M Marey, Mohamed A Dkhil, Ahmed A Abdel Moneim, Simeon Santourlidis, Rewaida Abdel-Gaber, Mohammed I Alquraishi, Mohga S Abdalla","doi":"10.1002/jemt.24691","DOIUrl":"https://doi.org/10.1002/jemt.24691","url":null,"abstract":"<p><p>Sepsis is a potentially fatal disease that arises from an infection and is characterized by an uncontrolled immune system reaction. Global healthcare systems bear a heavy financial burden from treating sepsis. This study aimed to provide information on the effective properties of silver nanoparticles derived from pomegranate peel extract (P-AgNP) against sepsis-induced hepatic injury. P-AgNPs were spherical with a diameter of ~19 nm. The animals were placed into four groups, each with seven rats. Group 1 functioned as the control group, receiving only saline for 7 days. Group 2 received only P-AgNPs at a dose of 20 mg/kg. To induce sepsis, groups 3 and 4 were given an intraperitoneal injection of 200 mg/mL cecal slurry. Sixty min later, group 4 was given 20 mg/kg of P-AgNPs daily for 7 days. The concentrations of reduced glutathione, nitric oxide, lipid peroxidation, and superoxide dismutase in liver homogenate were measured to determine the oxidative status. In addition, enzyme activities (alanine aminotransferase, aspartate amino transferase, and alkaline phosphatase) were measured. Furthermore, we investigated the histological changes, immunohistochemical expression of nuclear factor-κB, and mRNA levels of IL1β, IL-6, TNF-α, Bax, BCl2, and Casp-3. P-AgNPs functioned as regulators in a sepsis model, successfully controlling altered gene expression. Following treatment, P-AgNPs improved tion and oxidative state, indicating a role in sepsis management. Based on our findings, we conclude that P-AgNPs have antioxidant activity and may be useful in preventing sepsis-induced liver inflammation, oxidative damage, and apoptosis. RESEARCH HIGHLIGHTS: Pomegranate peel-derived silver nanoparticles (P-AgNPs) enhanced liver function and oxidative state in rats with sepsis-induced hepatic damage. P-AgNPs reduced oxidative stress and liver inflammation via regulating inflammatory and apoptotic gene expression. P-AgNPs enhanced liver enzyme activities, histological structure, and immunohistochemistry expression of nuclear factor-κB.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achene macro-micromorphology of Pulicaria (Asteraceae) from Turkiye and its taxonomic significance.","authors":"Bahar Gürdal, Ebru Özdemir Nath","doi":"10.1002/jemt.24690","DOIUrl":"https://doi.org/10.1002/jemt.24690","url":null,"abstract":"<p><p>The genus Pulicaria Gaertn. belongs to the tribe Inuleae Cass. and it is represented by six species P. arabica (L.) Cass., P. armena Boiss. & Kotschy ex Boiss., P. dysenterica (L.) Gaertn., P. odora (L.) Rchb., P. sicula (L.) Moris, and P. vulgaris Gaertn. in Turkiye. P. armena is endemic to the country. In this study, fruit macro-micromorphological characteristics of six Pulicaria species from Turkiye were demonstrated using stereo-microscope and scanning electron microscopy. The colors of the achenes range from light-brown to brown. The shapes are obovate to elliptic in outline. The pappus is persistent in all studied species, with two rows of pappus hairs except in P. sicula. Its pappus composed of one row of capillary bristles. Others have the outer row developing into a small crown and the inner row consisting of capillary bristles. All taxa have glandular trichomes, except for P. arabica. An identification key is also provided to differentiate between Pulicaria species. Fruit features provide a useful tool for differentiating the examined species. RESEARCH HIGHLIGHTS: Achene macro-micromorphology of Pulicaria species was studied via LM and SEM. A diagnostic key based on achene features was created for Pulicaria species. Achene features provide a useful tool for differentiating the examined species.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reveal of relationship between microscopy architecture and mechanical performance of Y/Bi substituted Bi-2212 engineering ceramics.","authors":"Tolgahan Yilmaz, Gülnur Kurtul, Asaf Tolga Ülgen, Ümit Erdem, Ali Mercan, Tahsin Turgay, Gürcan Yildirim","doi":"10.1002/jemt.24688","DOIUrl":"https://doi.org/10.1002/jemt.24688","url":null,"abstract":"<p><p>This study aims to find out how the crystallinity quality, surface morphology, and mechanical performances change with the substitution of yttrium (Y) for bismuth (Bi) impurity within molar ratios of 0.00 ≤ x ≤ 0.12 in the Bi_2.0-xY_xSr_2.0Ca_1.1Cu_2.0O_y (Bi-2212) cuprates to reveal the dependence of micro surface topology on the substitution mechanism and achieve a strong relation between the impurity ions and crystallization mechanism. The materials are prepared by ceramic method. It is found that all the experimental findings improve remarkably with increasing yttrium impurity molar ratio of x = 0.01. Scanning electron microscopy (SEM) images indicate that the optimum Y ions strengthen the formation of flaky adjacent stacked layers due to the changes of thermal expansion, vibration amplitude of atoms, heat capacitance, reaction kinetics, activation energy, nucleation temperature, thermodynamic stability, and intermolecular forces. Besides, new engineering novel compound produced by optimum Y ions presents the best crystallinity quality, uniform surface view, greatest coupling interaction between grains, largest particle size distributions/orientations, and densest/smoothest surface morphology. Hardness measurement results totally support the surface morphology view. Moreover, mechanical design properties and durability of the tetragonal phase improve significantly with increasing replacement level of x = 0.01 due to the induction of new surface residual compressive stress areas, slip systems, and chemical bonding between the foreign and host atoms. Besides, the same sample exhibits the maximum strength and minimum sensitivity to loads depending on reduction of stored internal strain energy and degree of granularity. Consequently, cracks tend to propagate predominantly within the transcrystalline regions. Furthermore, each material investigated exhibits the characteristic behavior of the indentation size effect. In summary, the optimum Y-doped Bi-2212 sample paves the way for the expanded use of engineering ceramics across various applications based on the enhanced service life. RESEARCH HIGHLIGHTS: The presence of the optimum yttrium impurity significantly decreases the E_a value. As the Y/Bi replacement increases up to the molar substitution level of x = 0.01, the mechanical design properties and durability of the tetragonal phase enhance significantly.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Morchella galilaea, an autumn species from Pakistan, based on multilocus DNA sequences, light microscopy, and scanning electron microscopy analyses.","authors":"Surat Un Nisa, Hussain Badshah, Sayed Afzal Shah, Abdul Samad Mumtaz","doi":"10.1002/jemt.24683","DOIUrl":"https://doi.org/10.1002/jemt.24683","url":null,"abstract":"<p><p>Morchella is highly prized for its distinct and delicious taste, making it one of the most sought-after and valuable macrofungi. Despite Pakistan exporting morels to various European countries, Morchella's diversity in this South Asian country has not been cataloged using multilocus DNA sequence data. Realizing the need for their scientific identity, various collections were commissioned, including those from the low and high altitudinal zones of the Charsadda, Swat, and Poonch districts of Pakistan during autumn of 2015 through 2019. Specimens were identified by sequencing the internal transcribed spacer (ITS) region and portions of the RNA polymerase II largest subunit (RPB1), second largest subunit (RPB2), and translation elongation factor 1-α (TEF1) loci. Multigene molecular phylogenetic analysis revealed the autumnal collections were Morchella galilaea Masaphy & Clowez. The species was previously documented in the United States, China, Israel, and Turkey. A detailed taxonomic description, light and scanning electron microscopy, and multigene molecular phylogenetic analyses of the species are reported here for the first time from Pakistan. The study underscores the necessity for further surveys of unexplored areas of Pakistan and systematics of Morchella to augment species diversity and their geographic distribution. RESEARCH HIGHLIGHTS: Specimens of Morchella were collected from low and high altitudinal zones in Pakistan during the autumn. Specimens were assessed using multigene (ITS, RPB1, RPB2, and TEF1) molecular phylogenetic analyses for the first time in Pakistan. The specimens were identified as Morchella galilaea based on multigene molecular phylogenetic analysis. Updated taxonomic descriptions of macro- and microanatomical characters were provided for clarity and precision. Hitherto-unreported shapes of paraphyses and acroparaphyses are observed and thoroughly reported. Scanning electron microscopy (SEM) of an ascospore is provided.</p>","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of structural, optical, photocatalytic, and antibacterial properties of ZnO doped GO nanoparticles for environment applications.","authors":"K Kaviyarasu","doi":"10.1002/jemt.24672","DOIUrl":"https://doi.org/10.1002/jemt.24672","url":null,"abstract":"<p><p>As a result of their unique and novel properties, nanocomposites have found applications in a wide variety of fields. The purpose of this study is to demonstrate the ability to synthesize nanoparticles consisting of zinc oxide (ZnO) and graphene oxide (GO) via sol-gel techniques. An x-ray diffractometer (XRD) as well as a UV-visible spectrometer were used to determine the crystalline and optical characteristics of the prepared samples. A hexagonal wurtzite crystal structure was observed in both pure ZnO nanoparticles and those that contain GO based on XRD results. It was estimated that the average crystallite size is based on the broadening of x-ray lines. In comparison with pure ZnO, the antimicrobial properties were enhanced when GO was incorporated with ZnO. In addition, experiments on the absorption edge indicated the presence of a red shift as a result of the incorporation of GO. When GO is incorporated in quantitative amounts, the bandgap value of pure ZnO decreased. FTIR spectra exhibit a band of absorption at 486 cm^-1, which confirms Zn-O stretching in both samples. SEM images reveal a random pattern of structural features on the surface of the prepared samples. According to the EDX spectrum, pure GO nanoparticles and those doped with ZnO contain 61%-64% zinc and 32%-34% oxygen, respectively. When annealed at a higher temperature, ZnO NPs produced more H₂ with a narrower bandgap than before annealing. In addition, methyl blue (MB) was used as an example of an organic compound in order to investigate the potential photocatalytic properties of nanoparticles with ZnO doped GO. In addition to DPPH assays, ZnO nanoparticles and ZnO doped GO nanoparticles were tested for their ability to scavenge free radicals. Comparing ZnO doped GO NPs with pure ZnO, these nanoparticles showed increased antioxidant activity. Based on the increased zone of inhibition observed for pure ZnO and ZnO doped GO (5, 10, 50, and 100 mg/mL), the antibacterial activity of pure ZnO and ZnO doped GO is concentration dependent. A detailed discussion of the results of the study demonstrated that ZnO doped GO and pure ZnO are toxic in different ways depending on how long they survive in degreased Zebrafish embryos and how fast they decompose. RESEARCH HIGHLIGHTS: The scope of the manuscript was under the results of the study confirmed that both nanoparticles exhibited concentration dependent antioxidative activity. Determined that 89% of methyl orange dye can be degraded photocatalytically. ZnO nanoparticles were found to be 74.86% antioxidant at a concentration of 50 g/mL in the present study. At a concentration of 50 g/mL, ZnO doped GO NPs showed 79.1% antioxidant activity. Photocatalytic degradation mechanism scheme is implicit in the photoexcited charge carrier transportation path is observed for all the samples. Survival rate of zebrafish embryos was shown to decrease with increasing concentrations of ZnO and zinc oxide plus GO nanoparticles.","PeriodicalId":18684,"journal":{"name":"Microscopy Research and Technique","volume":null,"pages":null},"PeriodicalIF":2.0,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142080885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}