Microbiology and Immunology最新文献

{"title":"Genetic Characterization of Avian Rotavirus A From Japanese Pigeons","authors":"Masaji Mase,&nbsp;Tohru Suzuki","doi":"10.1111/1348-0421.70062","DOIUrl":"10.1111/1348-0421.70062","url":null,"abstract":"<div>\u0000 \u0000 <p>Rotavirus A infects many animal species, including humans, and causes severe diarrhea and acute gastrointestinal diseases. This virus also infects various bird species including pigeons; however, information on avian rotavirus A (ARVA) infection in pigeon populations in Japan is limited. Here, we retrospectively report the genetic characterization based on whole-genome sequences of two Japanese ARVA isolates from pigeons in 2012. The isolates were obtained by inoculation into primary chicken kidney cell cultures and subsequently subjected to near-complete genome sequencing using an NGS system. The genotype constellation of the two ARVA isolates was G18-P[17]-I4-R4-C4-M4-A4-N4-T4-E19-H4, which were identical to those derived from several pigeons. Genetic analysis based on each segment revealed that the genomic sequences of all 11 genes from these ARVAs are closely related to those of ARVAs from foreign countries such as Germany, Italy, or China rather than those of the Japanese ARVAs in previous reports. These data suggest an ancestor common to the ARVA analyzed in this study might be distributed and maintained worldwide. This information will help researchers better understand the evolution and epidemiology of ARVA in feral birds.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 7","pages":"346-353"},"PeriodicalIF":1.6,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147817572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information – Cover","authors":"","doi":"10.1111/1348-0421.70075","DOIUrl":"https://doi.org/10.1111/1348-0421.70075","url":null,"abstract":"<p><b>Cover photograph</b>: Immunohistochemical staining of S100A9 protein levels in control (Ctr), CRS without nasal polyps (CRSsNP), CRS with nasal polyps (CRSwNP) tissues. <i>Microbiol Immunol: 70:333-345</i>. Article link here\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 7","pages":"i-ii"},"PeriodicalIF":1.6,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.70075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148532616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Inflammatory Alarmin S100A9 Modulates Activation of Pro-Inflammatory Macrophage to Protect Nasal Epithelial Cells From LPS-Induced Epithelial–Mesenchymal Transition","authors":"Yunxiang Ji,&nbsp;Jia Luan,&nbsp;Fang Yuan,&nbsp;Zhao Wang,&nbsp;Ran Wei,&nbsp;Guangbin Sun","doi":"10.1111/1348-0421.70060","DOIUrl":"10.1111/1348-0421.70060","url":null,"abstract":"<p>Chronic rhinosinusitis with nasal polyps (CRSwNP) exhibits pronounced endotypic heterogeneity, with macrophages serving as key drivers of sustained mucosal inflammation. In this study, we identify S100A9 as a macrophage-derived alarmin that is markedly elevated in CRSwNP tissues. Integrative analyses of public bulk transcriptomic datasets and single-cell RNA-sequencing atlases demonstrated that S100A9 expression was predominantly enriched in macrophage clusters, where it showed strong co-expression with canonical M1-associated markers, while exhibiting limited expression in epithelial cell subsets. Spatial and correlation analyses further supported a close association between S100A9⁺ macrophages and epithelial barrier–related gene signatures. Functionally, shRNA-mediated silencing of S100A9 attenuated M1-like macrophage polarization, as evidenced by reduced expression of pro-inflammatory mediators and polarization markers, accompanied by a shift toward a less inflammatory macrophage phenotype. Conditioned media derived from S100A9-deficient macrophages significantly mitigated epithelial injury, leading to restoration of epithelial barrier integrity, as indicated by enhanced expression of tight junction proteins, including occludin and claudins. Importantly, S100A9 knockdown disrupted the pathogenic macrophage–epithelial inflammatory feedback loop, thereby dampening sustained inflammatory signaling and limiting epithelial barrier breakdown that perpetuates tissue damage in CRSwNP. Clinically, elevated S100A9 levels correlated with disease severity indices and effectively distinguished a macrophage-enriched inflammatory endotype of CRSwNP, highlighting S100A9 as both a mechanistic driver and a potential biomarker for disease stratification. Collectively, these findings position S100A9 as a mechanistic mediator and a promising therapeutic target for CRSwNP.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 7","pages":"333-345"},"PeriodicalIF":1.6,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.70060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147840157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring Rubella Immunity and Transmission in Central India Amidst Elimination Efforts","authors":"Vijay Pratap Singh,&nbsp;Kavita N. Singh,&nbsp;Deepica Tirkey,&nbsp;Nisha Verma,&nbsp;Surbhi Mishra,&nbsp;Mahendra J. Ukey,&nbsp;Vivek Chouksey,&nbsp;Lalit Kumar Sahare,&nbsp;Vaishnavi Batham,&nbsp;Mrigendra P. Singh,&nbsp;Sandeep Kumar,&nbsp;Pushpendra Singh","doi":"10.1111/1348-0421.70065","DOIUrl":"10.1111/1348-0421.70065","url":null,"abstract":"<div>\u0000 \u0000 <p>India along with other countries in the World Health Organization (WHO) Southeast Asia region has set a goal to eliminate rubella by December 2026 through active tracking and vaccination program. However, the incidence of sporadic events and outbreaks in recent years has posed a significant challenge to this goal. To access the ongoing infections and the effectiveness of immunization efforts, we evaluated the presence of anti-rubella IgM and IgG antibodies, which serve as indicators of ongoing infection and immune protection, respectively. Serum samples from individuals presenting with suspected rubella symptoms were collected and referred to the State Level Virus Research and Diagnostic Laboratory (SL-VRDL) at ICMR-NIRTH, Jabalpur between 1st May 2023 to 30th April 2024 for anti-rubella IgM and IgG analysis. Concurrently, samples from healthy pregnant women, attending Netaji Subhash Chandra Bose Medical College &amp; Hospital, Jabalpur were obtained during the same period to assess the prevalence of maternal immunity against rubella. Collected samples were tested for rubella specific IgM and IgG antibody using ELISA. Rubella IgM seropositivity rate during the study period was 7.2% across all age groups, indicating ongoing transmission of rubella in central India. Rubella IgG seropositivity, reflecting immunity, was 81.0%, among all age groups, demonstrating a high level of immunity against the rubella virus in this region. Among pregnant women, rubella IgG seropositivity was 89.6%, suggesting that most pregnant women of reproductive age are having protection against rubella infection. The finding indicates that rubella elimination efforts are on the right track, and that the vaccination program is broadly effective. However, continuous detection of IgM positive cases, particularly in young children, highlights, immunity gaps, that may hinder timely elimination. These gaps could be due to logistical, cultural, and educational barriers and require further intensive efforts towards active immunization. Targeted and intensified immunization efforts are essential to achieve the elimination goal by 2026.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 7","pages":"354-359"},"PeriodicalIF":1.6,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antigenic Multi-Epitope Vaccine Against Ross River Virus Using Capsid and Envelope Proteins via Immunoinformatics and Reverse Vaccinology Approaches.","authors":"Elham Mohammed Khatrawi, Syed Luqman Ali, Hind Althagafi, Huda A Alqahtani","doi":"10.1111/1348-0421.70070","DOIUrl":"https://doi.org/10.1111/1348-0421.70070","url":null,"abstract":"<p><p>Ross River virus (RRV), a mosquito-borne alphavirus of the Togaviridae family, is the causative agent of Ross River fever a debilitating infectious disease characterized by fever, rash, and persistent polyarthritis. The virus remains endemic across Australia and the Pacific regions, with sporadic outbreaks reported in new geographic areas due to climate change and increased mosquito activity. Despite its growing public health impact, no licensed vaccine or antiviral therapy currently exists, underscoring the urgent need for an effective preventive strategy. In this study, an immunoinformatics-based approach was employed to design a novel multi-epitope vaccine (MEV) against RRV, where conserved and antigenic cytotoxic T-lymphocyte (CTL), helper T-lymphocyte (HTL), and B-cell epitopes were predicted from structural proteins (capsid and envelope glycoproteins E1 and E2) as well as selected non-structural protein regions, followed by their rational assembly into a vaccine construct using appropriate linkers and an immune-stimulatory adjuvant. Conserved and antigenic cytotoxic T-lymphocyte (CTL), helper T-lymphocyte (HTL), and B-cell epitopes were predicted from structural and non-structural proteins and linked with appropriate linkers, while β-defensin was incorporated as an adjuvant to enhance immunogenicity. The vaccine construct underwent modeling, refinement, and structural validation using PSIPRED, AlphaFold, and GalaxyRefine. Molecular docking analyses with immune receptors (TLR8 and HLA) and molecular dynamics (MD) simulations evaluated receptor binding affinity and structural stability. The C-ImmSim server was used to simulate immune responses, and codon optimization ensured suitability for Escherichia coli expression. The designed MEV exhibited strong antigenicity (0.79), stability, and solubility, with a molecular weight of ~30 kDa and theoretical pI of 9.98. Structural validation revealed over 95% of residues in favored Ramachandran regions, and docking analysis showed predicted interaction with TLR8 (- 1712.2 kJ/mol). MD simulations confirmed stable complex formation, while immune simulations predicted robust and long-lasting humoral and cellular immune responses with elevated IgG, IFN-γ, and memory B/T-cell populations. The designed MEV demonstrates promising antigenic, structural, and immunological properties, making it a potential vaccine candidate for further in vitro and in vivo evaluation against Ross River virus infection.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148302324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information – Cover","authors":"","doi":"10.1111/1348-0421.70066","DOIUrl":"https://doi.org/10.1111/1348-0421.70066","url":null,"abstract":"<p><b>Cover photograph</b>: Immunofluorescence images of HHV-6B-infected MT-4-shNT, MT-4-shU14-1, and MT 4-shU14-2 cells stained with an anti-IE1 antibody at 18 h post-infection. Bar; 100 μm. <i>Microbiol Immunol: 70:321-329</i>. Article link here\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 6","pages":"i-ii"},"PeriodicalIF":1.8,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148208957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term Promotion of Beneficial Skin Commensal Staphylococcus Species Growth by PS-B1, a Postbiotic Composed of Lactic Acid Bacteria-Fermented Soy Milk","authors":"Osamu Funatsu,&nbsp;Hiroko Ishii,&nbsp;Reiko Shimatsu,&nbsp;Yutaka Shimokawa,&nbsp;Akihiko Asahina,&nbsp;Itaru Dekio","doi":"10.1111/1348-0421.70057","DOIUrl":"10.1111/1348-0421.70057","url":null,"abstract":"<div>\u0000 \u0000 <p>Postbiotics, defined as non-viable microbial cells, their components, or metabolites, are emerging functional ingredients that can modulate host health, including skin barrier function, immune responses, and microbial balance. The human skin microbiome plays a critical role in maintaining skin health, and the selective stimulation of beneficial commensals is of growing interest for cosmetic applications. PS-B1, a postbiotic derived from lactic acid bacteria during soy fermentation, is already used in beverages and cosmetics, but its direct effects on skin-resident bacteria have not been fully elucidated. In this study, we evaluated the effects of preservative-containing and preservative-free PS-B1 on the growth of twelve <i>Staphylococcus</i> strains and seven <i>Cutibacterium acnes</i> strains <i>in vitro</i>. The preservative-containing PS-B1 showed strong growth inhibition for all strains at higher concentrations. In contrast, the preservative-free PS-B1 significantly promoted the growth of <i>Staphylococcus epidermidis</i>, <i>Staphylococcus hominis</i>, and <i>Staphylococcus lugdunensis</i>, while <i>Staphylococcus warneri</i> was the only strain exhibiting growth inhibition. Other species showed no notable changes. Additionally, all <i>C. acnes</i> strains displayed slight growth enhancement. These results indicate that preservative-free PS-B1 selectively stimulates beneficial skin commensals, particularly <i>S. hominis</i>, revealing a previously unrecognized potential of PS-B1 to support a balanced skin microbiome.</p></div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 6","pages":"293-304"},"PeriodicalIF":1.8,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC3-Associated Phagocytosis in Hepatitis C Virus–Induced Fibrosis","authors":"Nermine Magdi Riad,&nbsp;Zainab Wafik Masoud,&nbsp;Yasmine Gaber,&nbsp;Rabab El Hawary,&nbsp;Ayman Yosry,&nbsp;Hend H. Tamim,&nbsp;Naglaa Ali Zayed,&nbsp;Mariam Onsy F. Hanna","doi":"10.1111/1348-0421.70058","DOIUrl":"10.1111/1348-0421.70058","url":null,"abstract":"<div>\u0000 \u0000 <p>During LC3-associated phagocytosis (LAP), a non-canonical form of autophagy, LC3 is recruited to pathogen and apoptotic cells containing phagosomes, where the phagocytosed cargo is degraded. Given the importance of LAP in host defense and the role of phagocytic cells in liver inflammation and fibrosis, this study assessed LAP in peripheral blood neutrophils and monocytes from patients with variable degrees of liver fibrosis and cirrhosis secondary to viral hepatitis C infection. LAP was quantified by flow cytometry using labeled zymosan as a stimulus to engage LAP and LC3 antibodies to detect LC3 recruitment. LAP engagement was evaluated as the integrated mean fluorescence intensity of LC3 in zymosan-positive cells. LAP was enhanced in patients with mild to moderate liver fibrosis (stages F1–F2) compared with patients with no fibrosis (F0) and severe fibrosis or cirrhosis (stages F3–F4). Furthermore, LAP in both neutrophils and monocytes correlated with the stage of fibrosis from F0 to F2, indicating that as the process of fibrosis progressed in the early stages, LAP activity increased. No differences in LAP were observed between treatment-naïve and those who received direct acting antiviral therapy and achieved sustained virologic response (SVR), suggesting that the process of fibrosis had a more significant impact on LAP, rather than the presence or absence of HCV infection. In conclusion, LAP is enhanced in circulating phagocytes during early stages of HCV-related fibrosis but declines in advanced disease, independent of treatment status and SVR.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 6","pages":"315-320"},"PeriodicalIF":1.8,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conserved U14-Mediated NF-κB Activation in Human Herpesvirus 6A and 6B","authors":"Khoir Amaliin,&nbsp;Aila Gulijiahani,&nbsp;Mansaku Hirai,&nbsp;Yasuko Mori,&nbsp;Jun Arii","doi":"10.1111/1348-0421.70059","DOIUrl":"10.1111/1348-0421.70059","url":null,"abstract":"<div>\u0000 \u0000 <p>Human herpesvirus 6B (HHV-6B) is the most prevalent HHV-6 species in humans and is associated with roseola infantum, febrile seizures, and increased morbidity following reactivation in immunocompromised patients. Herpesviruses frequently modulate NF-κB, a central regulator of inflammatory gene expression, to promote viral gene expression and replication. The HHV-6A tegument protein U14 activates NF-κB, but whether this activity is conserved in HHV-6B and contributes to infection-associated outputs has remained unclear. Here, we evaluated HHV-6A and HHV-6B U14 in a transient NF-κB reporter assay and examined U14 function during HHV-6B infection using two independent U14-targeting shRNAs. Both U14 orthologs activated an NF-κB reporter. During HHV-6B infection in MT-4 cells, U14 knockdown reduced phosphorylation of p65 (Ser536) and was accompanied by decreased mRNA levels of the immediate-early viral gene IE2 and the early viral genes U27 and U38, as well as reduced release of viral genomes into culture supernatants. U14 knockdown also reduced IL-2, IL-6, and IL-8 transcript levels, consistent with attenuation of an NF-κB–linked cytokine transcriptional program. Together, these results extend U14-mediated NF-κB activation to HHV-6B and link U14 depletion to reduced viral gene expression and productive replication.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 6","pages":"321-329"},"PeriodicalIF":1.8,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Novel Real-Time PCR Assay Differentiating Burkholderia mallei From Burkholderia pseudomallei","authors":"Ai Koshikawa,&nbsp;Yoshiki Ichikawa,&nbsp;Mitsuru Nakase,&nbsp;Apichai Tuanyok,&nbsp;Vannarat Saechan,&nbsp;Keisuke Aoshima,&nbsp;Hideaki Higashi,&nbsp;Chie Nakajima,&nbsp;Yasuhiko Suzuki,&nbsp;Vanaabaatar Batbaatar,&nbsp;Takashi Kimura","doi":"10.1111/1348-0421.70061","DOIUrl":"10.1111/1348-0421.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Glanders is a zoonotic disease caused by <i>Burkholderia mallei</i> (Bm). Bm is phylogenetically similar to <i>B. pseudomallei</i> (Bp), the causative agent of melioidosis, making it difficult to differentiate between them. Recently, the PCR method recommended by the World Organisation for Animal Health (WOAH) failed to detect a Kuwait strain of Bm, and the WOAH has cautioned that ongoing Bm evolution may lead to the emergence of variants that escape standard assays. Therefore, this study aimed to develop a novel real-time PCR method to discriminate between Bm and Bp. Local and web-based BLAST searches identified a 494-bp sequence conserved across Bm but absent from other bacterial genomes, including Bp, except for one <i>B. thailandensis</i> strain. The optimized probe-based real-time PCR targeting this sequence detected the genomes of five Bm isolates but did not amplify DNA from closely related <i>Burkholderia</i> species. In clinical specimens from Mongolia, this assay amplified Bm DNA at an equivalent detection rate to the WOAH-recommended real-time PCR method. These findings provide a novel, highly sensitive diagnostic method for differentiating glanders from melioidosis and suggest its potential use as an alternative method in clinical applications. Further verification using a large number of samples collected from various regions will be necessary in the future.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 6","pages":"305-314"},"PeriodicalIF":1.8,"publicationDate":"2026-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}