An Atypical Kappa-Class Chaperone-Usher Fimbriae of a Human Enterotoxigenic Escherichia coli Strain Shows Multi-Host Adherence and Distinct Phylogenetic Feature.
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引用次数: 0
Abstract
The pathogenesis of enterotoxigenic Escherichia coli (ETEC) involves the colonization of hosts by colonization factors (CFs) and the secretion of enterotoxins. CFs, especially chaperone-usher fimbriae, mediate bacterial adhesion to host cells, with extensive genetic diversity observed among isolates. One ETEC strain, O169YN10, possessed a unique plasmid (pEntYN10) encoding three CFs, CS6, and two novel homologs of CS8 and F4 (CS6O169, CS8O169, and F4O169). In this study, F4O169 was found to play a major role in adhesion to multiple hosts, including human, bovine, and porcine epithelial cells, whereas the other two CSs were less functional. Inhibition assays using antibodies showed that FayG1, one of the two major paralogous adhesins of F4O169, directly contributes to human cell adhesion. Despite the established function of FayG1, the FayG2 protein was not detected under the in vitro conditions. Comparative genomics revealed that FayG1 and FayG2 share low homology with other E. coli strains isolated from hosts, suggesting sporadic emergence from an unknown origin.
期刊介绍:
Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses.
Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.