An Atypical Kappa-Class Chaperone-Usher Fimbriae of a Human Enterotoxigenic Escherichia coli Strain Shows Multi-Host Adherence and Distinct Phylogenetic Feature.

IF 1.9 4区 医学 Q4 IMMUNOLOGY
Hiharu Inoue, Yoshihiko Tanimoto, Dongming Zheng, Erika Ban-Furukawa, Miyoko Inoue, Yuko Omori, Yoshihiro Yamaguchi, Taro Tachibana, Hisashi Aso, Weiping Zhang, Eriko Kage-Nakadai, Yoshikazu Nishikawa, Takayuki Wada
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Abstract

The pathogenesis of enterotoxigenic Escherichia coli (ETEC) involves the colonization of hosts by colonization factors (CFs) and the secretion of enterotoxins. CFs, especially chaperone-usher fimbriae, mediate bacterial adhesion to host cells, with extensive genetic diversity observed among isolates. One ETEC strain, O169YN10, possessed a unique plasmid (pEntYN10) encoding three CFs, CS6, and two novel homologs of CS8 and F4 (CS6O169, CS8O169, and F4O169). In this study, F4O169 was found to play a major role in adhesion to multiple hosts, including human, bovine, and porcine epithelial cells, whereas the other two CSs were less functional. Inhibition assays using antibodies showed that FayG1, one of the two major paralogous adhesins of F4O169, directly contributes to human cell adhesion. Despite the established function of FayG1, the FayG2 protein was not detected under the in vitro conditions. Comparative genomics revealed that FayG1 and FayG2 share low homology with other E. coli strains isolated from hosts, suggesting sporadic emergence from an unknown origin.

人类肠毒性大肠埃希氏菌株的一种非典型 Kappa-Class 伴孢子-胞膜具有多宿主黏附性和独特的系统发育特征。
肠毒性大肠埃希氏菌(ETEC)的致病机制包括定植因子(CFs)在宿主体内的定植和肠毒素的分泌。定殖因子,尤其是合子推力缘膜,介导细菌粘附到宿主细胞,在分离菌株中观察到广泛的遗传多样性。一株名为 O169YN10 的 ETEC 菌株拥有一个独特的质粒(pEntYN10),编码三种 CFs,即 CS6 以及 CS8 和 F4 的两个新型同源物(CS6O169、CS8O169 和 F4O169)。本研究发现,F4O169 在粘附多种宿主(包括人、牛和猪上皮细胞)时发挥了主要作用,而其他两个 CS 的功能较弱。使用抗体进行的抑制试验表明,F4O169 的两个主要同源粘附蛋白之一 FayG1 直接参与了人类细胞的粘附。尽管 FayG1 的功能已经确定,但在体外条件下却检测不到 FayG2 蛋白。比较基因组学发现,FayG1 和 FayG2 与从宿主体内分离出来的其他大肠杆菌菌株同源性较低,这表明它们是零星出现的,来源不明。
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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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