Microbiology and Immunology最新文献_第5页

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-05-05

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Increased Levels of High Mobility Group Box 1 in the Bronchoalveolar Lavage Fluid of Murine Pneumococcal Pneumonia Model. 小鼠肺炎球菌肺炎模型支气管肺泡灌洗液高迁移率组1水平升高

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-05-01 Epub Date: 2026-03-20 DOI: 10.1111/1348-0421.70054

Fumio Takizawa, Hisanori Domon, Satoru Hirayama, Toshihito Isono, Rui Saito, Yoshihito Yasui, Mana Endo, Daisuke Yonezawa, Tomoki Maekawa, Koichi Tabeta, Yutaka Terao

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Distribution of Lantibiotic Susceptibility and Its Association With Two-Component Regulatory Systems in Staphylococcus aureus Clinical Isolates. 金黄色葡萄球菌临床分离株抗生素敏感性分布及其与双组分调控系统的关系

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-05-01 Epub Date: 2026-04-12 DOI: 10.1111/1348-0421.70056

Airi Odawara, Yujin Suzuki, Miki Kawada-Matsuo, Junzo Hisatsune, Chika Arai, Yo Sugawara, Motoyuki Sugai, Souichi Yanamoto, Hitoshi Komatsuzawa

{"title":"Distribution of Lantibiotic Susceptibility and Its Association With Two-Component Regulatory Systems in Staphylococcus aureus Clinical Isolates.","authors":"Airi Odawara, Yujin Suzuki, Miki Kawada-Matsuo, Junzo Hisatsune, Chika Arai, Yo Sugawara, Motoyuki Sugai, Souichi Yanamoto, Hitoshi Komatsuzawa","doi":"10.1111/1348-0421.70056","DOIUrl":"10.1111/1348-0421.70056","url":null,"abstract":"<p><p>Bacteriocins, antimicrobial peptides produced by bacteria, are expected to be novel antimicrobial agents. Previous studies demonstrated that several two-component regulatory systems (TCSs) linked to the expression of lantibiotic transporters are involved in resistance to lantibiotics in Staphylococcus aureus. In this study, we examined the susceptibility to four Class Ia bacteriocins (lantibiotics) among clinical isolates of S. aureus and sought to reveal the mechanisms underlying differences in susceptibility among strains. A direct assay was performed using nisin A, Pep5, nukacin ISK-1, and epidermin-producing strains to examine the susceptibility of 149 S. aureus isolates isolated from nasal cavity or bloodstream infections. Among them, some strains showed significantly higher susceptibility to epidermin, nukacin ISK-1, and nisin A. These strains did not show the induction of transporter-encoded vraD expression in the presence of nisin A, suggesting impaired TCS BraRS function. Some strains harbored frameshifts or nonsense mutations in braR, braA, and braB or the absence of transporter-encoded vraDEH genes. In addition, strains with frameshifts in TCS GraR and transporter VraG genes showed increased susceptibility to nisin A, Pep5, and nukacin ISK-1. When comparing the susceptibility among MLST-defined clonal complexes (CCs), CC45 showed higher susceptibility to nisin A, Pep5, and nukacin ISK-1, while CC5 exhibited low susceptibility to them. CC45 also showed higher susceptibility within the BraRS-disrupted strains. The net negative charge of the cell surface did not vary significantly among CCs, suggesting the involvement of factors other than BraRS and GraRS. These findings provide important insights for evaluating bacteriocins as novel antimicrobial agents against S. aureus.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":"268-279"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Emergence and Genomic Characterization of a Hypervirulent Klebsiella pneumoniae Isolate From a New Clone in Brazil. 巴西一个新克隆的高致病性肺炎克雷伯菌分离株的出现和基因组特征。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-05-01 Epub Date: 2026-03-27 DOI: 10.1111/1348-0421.70052

Carlos Henrique Camargo, Ana Beatriz Nascimento Costa, Amanda Yaeko Yamada, Daniel de Sena Miranda, Pedro Smith Pereira Ferraro, Andreia Rodrigues de Souza, Amanda Maria de Jesus Bertani, Karoline Rodrigues Campos, Marlon Benedito Nascimento Santos, Rafaela Mastrangelo Rissetti, Carolina Lechinski de Paula, Patrik Júnior de Lima Paz, Maria Solange Duarte Soares, Christiane Salvador, Giovanna de Pinho Pieri, Jorge Siguemassa Higa, Daniela Dos Santos Souza, Márcio Garcia Ribeiro, Claudio Tavares Sacchi, Monique Ribeiro Tiba-Casas, Geraldine Madalosso, Denise Brandao de Assis

{"title":"Emergence and Genomic Characterization of a Hypervirulent Klebsiella pneumoniae Isolate From a New Clone in Brazil.","authors":"Carlos Henrique Camargo, Ana Beatriz Nascimento Costa, Amanda Yaeko Yamada, Daniel de Sena Miranda, Pedro Smith Pereira Ferraro, Andreia Rodrigues de Souza, Amanda Maria de Jesus Bertani, Karoline Rodrigues Campos, Marlon Benedito Nascimento Santos, Rafaela Mastrangelo Rissetti, Carolina Lechinski de Paula, Patrik Júnior de Lima Paz, Maria Solange Duarte Soares, Christiane Salvador, Giovanna de Pinho Pieri, Jorge Siguemassa Higa, Daniela Dos Santos Souza, Márcio Garcia Ribeiro, Claudio Tavares Sacchi, Monique Ribeiro Tiba-Casas, Geraldine Madalosso, Denise Brandao de Assis","doi":"10.1111/1348-0421.70052","DOIUrl":"10.1111/1348-0421.70052","url":null,"abstract":"<p><p>Hypervirulent Klebsiella pneumoniae (hvKp) has emerged globally as a major public health concern, often associated with severe community-acquired and healthcare-associated human infections. Despite alerts from PAHO/WHO on convergent hvKp strains with multidrug resistance in Latin America, comprehensive data from Brazil, the region's largest country, remain lacking. We performed a retrospective and prospective large-scale surveillance of clinical, veterinary, and community isolates to identify hypervirulent and resistant K. pneumoniae. Among 1,008 isolates screened, one strain (ID_074_25) met hvKp criteria, displaying key virulence loci. Whole-genome sequencing confirmed its genomic features, including a novel sequence type and a novel cluster in NCBI Pathogen Detection tool. This study underscores the need for national surveillance and capacity-building in reference laboratories.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":"261-267"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147531024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Establishment and Evaluation of Human iPS Cell-Derived Myeloid Cell Lines Susceptible to SARS-CoV-2. SARS-CoV-2易感人iPS细胞衍生髓系的建立与评价

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-05-01 Epub Date: 2026-03-10 DOI: 10.1111/1348-0421.70053

Takayuki Hishiki, Yuka Yoshimura, Rieko Suzuki, Ami Murakami, Yua Saito, Naomi Tanga, Jun Shimizu, Koichi Watashi, Tomohiko Takasaki, Kazuo Miyazaki

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IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-12

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Elevated Level of miR-711 Is Associated With Inflammation and Disease Progression in Patients With Severe Acute Pancreatitis miR-711水平升高与严重急性胰腺炎患者的炎症和疾病进展相关

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-01-28 DOI: 10.1111/1348-0421.70044

Huili Chen, Changjing Qu, Xiufeng Pang, Xuan Wang, Ju Tian, Zhihua Tao, Feng Zhu

{"title":"Elevated Level of miR-711 Is Associated With Inflammation and Disease Progression in Patients With Severe Acute Pancreatitis","authors":"Huili Chen, Changjing Qu, Xiufeng Pang, Xuan Wang, Ju Tian, Zhihua Tao, Feng Zhu","doi":"10.1111/1348-0421.70044","DOIUrl":"10.1111/1348-0421.70044","url":null,"abstract":"<div>\u0000 \u0000 <p>Severe acute pancreatitis (SAP) is a critical abdominal emergency, with inflammatory response being the core pathogenic mechanism. This study aims to explore the role of miR-711 in SAP and its underlying mechanisms. This study enrolled 121 MAP patients and 109 SAP patients, collecting their clinical data. qRT-PCR measured miR-711 expression in serum and cells. Cell viability was tested with CCK-8, inflammatory factors via ELISA kits, and oxidative stress using SOD and MDA kits. The dual-luciferase assay confirmed miR-711 and KRT8 binding. Compared to the mild acute pancreatitis (MAP) group, the expression level of miR-711 in the serum of patients in the SAP group was significantly upregulated and positively correlated with relevant indicators. The ROC curve demonstrated its potential diagnostic value for SAP. In vitro, the miR-711 inhibitor effectively enhances the viability of Caerulein-induced AR42J cells and alleviates their inflammation and oxidative damage. Furthermore, the study confirmed that KRT8 is a downstream target gene of miR-711, and there is a negative correlation between their expressions. KRT8 may reverse the protective effect of miR-711 inhibitor on Cerulein-induced AR42J cells by regulating the NF-κB and p38 MAPK signaling pathways. The serum level of miR-711 in SAP patients is significantly upregulated and negatively correlated with SAP disease-related indicators. In vitro models demonstrate that inhibition of miR-711 effectively alleviates cerulein-induced cellular damage. Overexpression of KRT8 can reverse the cellular damage caused by miR-711 overexpression.</p></div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"222-231"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Integrated Computational and In Vivo Assessment of a Novel Multi-Epitope Vaccine Against Dengue Virus Serotype 4 Capsid Protein 针对登革热病毒血清型4衣壳蛋白的新型多表位疫苗的综合计算和体内评估

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-02-12 DOI: 10.1111/1348-0421.70048

Elham Mohammed Khatrawi, Syed Luqman Ali, Rasha Alonaizan, Syed Mudasser Ali, Muhammad Kashif Obaid

{"title":"Integrated Computational and In Vivo Assessment of a Novel Multi-Epitope Vaccine Against Dengue Virus Serotype 4 Capsid Protein","authors":"Elham Mohammed Khatrawi, Syed Luqman Ali, Rasha Alonaizan, Syed Mudasser Ali, Muhammad Kashif Obaid","doi":"10.1111/1348-0421.70048","DOIUrl":"10.1111/1348-0421.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>Dengue Virus Serotype 4 (DENV4) continues to contribute substantially to global dengue morbidity, yet current tetravalent vaccines provide inconsistent protection and may pose risks of antibody-dependent enhancement (ADE). This study aimed to design and evaluate a serotype-specific multi-epitope vaccine (MEV-DV4) targeting the conserved capsid protein of DENV4 using integrated reverse vaccinology and immunoinformatics approaches. Conserved, antigenic, non-allergenic, and non-toxic B-cell, cytotoxic T lymphocyte (CTL), and helper T lymphocyte (HTL) epitopes were identified and assembled with a β-defensin adjuvant and PADRE sequence using optimized linkers. The final construct demonstrated high antigenicity (0.8559), structural stability, favorable physicochemical properties, and excellent solubility. Structural validation confirmed 97.6% of residues in favored Ramachandran regions. Molecular docking revealed strong interactions with TLR4 and TLR8, particularly MEV–TLR8, and molecular dynamics simulations supported the overall stability of the complex. Immune simulations predicted robust humoral and cellular responses with strong memory formation. Experimental validation in an albino mouse model using an alum-adjuvanted MEV-DV4 formulation showed early and potent antibody responses, with peak HI titers at day 21. Notably, antibody titers induced by MEV-DV4 were statistically comparable to those produced by a commercial inactivated dengue vaccine at all tested time points (<i>p</i> > 0.05), while no adverse reactions were observed. These computational and experimental findings demonstrate that MEV-DV4 is a safe, immunogenic, and promising serotype-specific vaccine candidate against DENV4. Further neutralization and challenge studies are warranted to advance its preclinical development.</p></div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"241-258"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Detection of a Novel Parahenipavirus From Northern Short-Tailed Shrews (Blarina brevicauda [Say, 1823]) 北方短尾鼩（Blarina brevicauda）中一种新型旁希尼帕病毒的检测[Say， 1823]。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-01-23 DOI: 10.1111/1348-0421.70043

Sakiho Imai, Mai Kishimoto, Masayuki Horie

{"title":"Detection of a Novel Parahenipavirus From Northern Short-Tailed Shrews (Blarina brevicauda [Say, 1823])","authors":"Sakiho Imai, Mai Kishimoto, Masayuki Horie","doi":"10.1111/1348-0421.70043","DOIUrl":"10.1111/1348-0421.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>Parahenipaviruses (genus <i>Parahenipavirus</i>) are nonsegmented negative-strand RNA viruses belonging to the family <i>Paramyxoviridae</i> of the order <i>Mononegavirales</i>. Parahenipaviruses have been detected in shrews and rodents, some of which have been reported to be associated with human diseases. Although many parahenipaviruses have been identified thus far, there still exist large phylogenetic gaps in parahenipaviruses, suggesting the existence of yet-to-be-identified parahenipaviruses. In this study, we analyzed public RNA-seq data and identified a novel parahenipavirus from northern short-tailed shrews (<i>Blarina brevicauda</i> [Say, 1823]), tentatively named Blarina brevicauda virus (BbV). Based on sequence comparisons between BbV and its most closely related viruses, BbV meets the International Committee on Taxonomy of Viruses species demarcation criteria, and therefore we propose that BbV is a novel species of virus in the genus <i>Parahenipavirus</i>. Furthermore, mapping analysis using RNA-seq data derived from multiple tissues of northern short-tailed shrews suggested a possible kidney tropism of BbV. This study provides novel insights into the diversity of parahenipaviruses, which would contribute to a deeper understanding of their evolution and control of infectious diseases.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"214-221"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Information – Cover 发行资料-封面

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 DOI: 10.1111/1348-0421.70055

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