Robert H Lampe, Ariel J Rabines, Bryce A Ellman, Hong Zheng, Andrew E Allen
{"title":"Marine microbial mock communities for validating rRNA gene amplicon sequencing.","authors":"Robert H Lampe, Ariel J Rabines, Bryce A Ellman, Hong Zheng, Andrew E Allen","doi":"10.1128/mra.00298-25","DOIUrl":"https://doi.org/10.1128/mra.00298-25","url":null,"abstract":"<p><p>Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0029825"},"PeriodicalIF":0.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete genome sequence of <i>Gulosibacter</i> sp. ACHW.36C, a toluene-degrading strain isolated in Hong Kong.","authors":"A C H Wai, G K K Lai, F C C Leung, S D J Griffin","doi":"10.1128/mra.00304-25","DOIUrl":"https://doi.org/10.1128/mra.00304-25","url":null,"abstract":"<p><p><i>Gulosibacter</i> spp. are known as consumers of xenobiotics and, though widely distributed in the environment, are infrequently reported. <i>Gulosibacter</i> sp. ACHW.36C was isolated from a cleaning sponge contaminated with a petroleum-based hoof oil, and its complete genome (2,748,452 bp, 67.61% G+C) established through hybrid assembly.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0030425"},"PeriodicalIF":0.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Coding-complete genome sequence of an <i>Alphacoronavirus</i> isolated in <i>Rattus norvegicus</i> captured in an urban park in France.","authors":"Kevyn Beissat, Evelyne Picard-Meyer, Virginie Lattard, Fanny Bastien, Jean-Luc Schereffer, Sionfoungo Daouda Soro, Elodie Monchâtre-Leroy, Marine Wasniewski","doi":"10.1128/mra.00230-25","DOIUrl":"https://doi.org/10.1128/mra.00230-25","url":null,"abstract":"<p><p>Here, we described an <i>Alphacoronavirus</i> coding-complete genome identified in a French brown rat. This <i>Alphacoronavirus</i> was detected in <i>Rattus norvegicus</i> intestines (colon) collected in 2022 in Marseille, France.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0023025"},"PeriodicalIF":0.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>De novo</i> assembly of multidrug resistant biofilm forming <i>Micrococcus luteus</i> genome from hemodialysis tunneled cuffed catheter tips of patients undergoing renal failure treatment.","authors":"Rajsekhar Adhikary, Indrani Sarkar, Dhara Patel, Sishir Gang, Uttam Kumar Nath, Saugata Hazra","doi":"10.1128/mra.00095-25","DOIUrl":"https://doi.org/10.1128/mra.00095-25","url":null,"abstract":"<p><p><i>Micrococcus luteus</i> HL_Chru_C<sub>3</sub> was isolated from the hemodialysis tunneled cuffed catheter tip of renal failure patients. Whole-genome sequencing (WGS) revealed a 2,494,573 bp genome with 12 contigs, 72% GC content, and 2,240 protein-coding genes. The computational prediction of penicillin-binding proteins and biofilm-forming signaling gene cassettes may contribute to the resistance mechanisms.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0009525"},"PeriodicalIF":0.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dikonketso Shirleymay Matjuda, Tshifhiwa Paris Mamphogoro
{"title":"<i>De novo</i> genome assessment of <i>Limosilactobacillus reuteri C4</i>, a potential probiotic lactic acid bacterium isolated from porcine Ileum.","authors":"Dikonketso Shirleymay Matjuda, Tshifhiwa Paris Mamphogoro","doi":"10.1128/mra.00823-24","DOIUrl":"10.1128/mra.00823-24","url":null,"abstract":"<p><p><i>Limosilactobacillus reuteri</i> is a lactic acid bacterium with several probiotic properties. Here, we present the draft genome sequence of <i>L. reuteri</i> isolated from the porcine ileum with a genome size of 2.1 Mb. The average genome sequence had a G + C content of 43.71% with 113 contigs, 54 RNA, and 2,012 protein-coding sequences.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0082324"},"PeriodicalIF":0.7,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Complete mitochondrial genome of <i>Melanabropsis tianmuica</i> (Anabropsinae: Anostostomatidae) from China.","authors":"Siyu Pang, Yingying Zhang, Yanting Qin, Xun Bian","doi":"10.1128/mra.00612-24","DOIUrl":"https://doi.org/10.1128/mra.00612-24","url":null,"abstract":"<p><p>The <i>Melanabropsis tianmuica</i> mitogenome was 15,696 bp in length and consisted of 13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes. All 13 protein-coding genes started with the ATN codons and terminated with TAA/TAG, except <i>cox2</i> and <i>nad5</i>, which ended with incomplete T.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0061224"},"PeriodicalIF":0.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexander Balkin, Andrey Plotnikov, Tatiana Konnova, Natalia Gogoleva, Elena Shagimardanova, Yuri Gogolev
{"title":"Cappable-seq RNA-sequencing data sets for comparative studying of <i>Salmonella enterica</i> adaptation to <i>Acanthamoeba castellanii</i> intracellular environment, oxidative stress, and starvation.","authors":"Alexander Balkin, Andrey Plotnikov, Tatiana Konnova, Natalia Gogoleva, Elena Shagimardanova, Yuri Gogolev","doi":"10.1128/mra.01129-24","DOIUrl":"https://doi.org/10.1128/mra.01129-24","url":null,"abstract":"<p><p><i>Salmonella</i> is a foodborne pathogen that can survive various stresses and replicate inside protist cells. Here, we present high-resolution Cappable-seq transcriptomic data for <i>Salmonella enterica</i> 14028S under starvation and hydrogen peroxide treatment, as well as 8 and 15 h after <i>Acanthamoeba castellanii</i> uptake.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0112924"},"PeriodicalIF":0.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angelina Garcia Guzman, Andrea Orozco, Haleema Amin, Rüdiger Pukall, Markus Goeker, Natalia Ivanova, Rekha Seshadri, Tricia A Van Laar
{"title":"Draft genome sequence of <i>Dolosicoccus paucivorans</i> DSM 15742<sup>T</sup> isolated from a patient's blood in Cleveland, Ohio.","authors":"Angelina Garcia Guzman, Andrea Orozco, Haleema Amin, Rüdiger Pukall, Markus Goeker, Natalia Ivanova, Rekha Seshadri, Tricia A Van Laar","doi":"10.1128/mra.01296-24","DOIUrl":"https://doi.org/10.1128/mra.01296-24","url":null,"abstract":"<p><p>This study describes the whole genome sequence of <i>Dolosicoccus paucivorans</i> 2991-95 (DSM 15742) isolated from human blood in Cleveland, Ohio. The genome length is approximately 1.8 Mbp with 107 contigs and a G + C content of 37.91%. Annotation identified 1,752 coding genes and multiple virulence factors.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0129624"},"PeriodicalIF":0.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saisuki Putumbaka, Claire E Barrow, Farris L Poole, Michael W W Adams
{"title":"Genome sequence of <i>Cetobacterium somerae</i> ATCC BAA-474 isolated from human feces.","authors":"Saisuki Putumbaka, Claire E Barrow, Farris L Poole, Michael W W Adams","doi":"10.1128/mra.01196-24","DOIUrl":"https://doi.org/10.1128/mra.01196-24","url":null,"abstract":"<p><p><i>Cetobacterium somerae</i> ATCC BAA-474 was first isolated from human feces and is emerging as an important species for animal and human health. Here, we report the complete genome sequence of this strain of <i>C. somerae</i> to improve the currently available genome sequence.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0119624"},"PeriodicalIF":0.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144160208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}