用于验证rRNA基因扩增子测序的海洋微生物模拟群落。

IF 0.7 Q4 MICROBIOLOGY

Microbiology Resource Announcements Pub Date : 2025-05-30 DOI:10.1128/mra.00298-25

Robert H Lampe, Ariel J Rabines, Bryce A Ellman, Hong Zheng, Andrew E Allen

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引用次数: 0

摘要

模拟社区是用于方法开发、测试或验证的细胞或DNA的混合物。在这里，我们提出了基于先前使用的来自不同海洋微生物的16S和18S rRNA基因序列的模拟群落。它们用作扩增子测序的控制，可以检测偏置或异常运行。

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Marine microbial mock communities for validating rRNA gene amplicon sequencing.

Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.

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来源期刊

Microbiology Resource Announcements MICROBIOLOGY-

CiteScore

1.50

自引率

12.50%

发文量

837