Robert H Lampe, Ariel J Rabines, Bryce A Ellman, Hong Zheng, Andrew E Allen
{"title":"Marine microbial mock communities for validating rRNA gene amplicon sequencing.","authors":"Robert H Lampe, Ariel J Rabines, Bryce A Ellman, Hong Zheng, Andrew E Allen","doi":"10.1128/mra.00298-25","DOIUrl":null,"url":null,"abstract":"<p><p>Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.</p>","PeriodicalId":18654,"journal":{"name":"Microbiology Resource Announcements","volume":" ","pages":"e0029825"},"PeriodicalIF":0.7000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology Resource Announcements","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1128/mra.00298-25","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Mock communities are defined mixtures of cells or DNA for methods development, testing, or validation. Here, we present mock communities based on previously used 16S and 18S rRNA gene sequences from diverse marine microorganisms. Their use as controls for amplicon sequencing enables the detection of biased or aberrant runs.