Microscopy and Microanalysis最新文献_第8页

Compositional Community Detection: Automated Identification of Chemical Segregation in Atom Probe Tomography Data. 成分群落检测：原子探针层析成像数据中化学分离的自动识别。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf036

Jenna A Bilbrey, Christina Doty, Mark G Wirth, Mengkong Tong, Jacqueline Royer, David J Senor, Arun Devaraj

{"title":"Compositional Community Detection: Automated Identification of Chemical Segregation in Atom Probe Tomography Data.","authors":"Jenna A Bilbrey, Christina Doty, Mark G Wirth, Mengkong Tong, Jacqueline Royer, David J Senor, Arun Devaraj","doi":"10.1093/mam/ozaf036","DOIUrl":"https://doi.org/10.1093/mam/ozaf036","url":null,"abstract":"We introduce a fully unsupervised clustering method we call Compositional Community Detection (CCD) to identify chemical motifs in atom probe tomography (APT) reconstructions. In the CCD approach, APT point clouds are broken into overlapping spherical neighborhoods, and repeated k-means clustering coupled with Louvain community detection is used to group neighborhoods based on their ion composition. Kolmogorov-Smirnov statistics for present ion types provide interpretable descriptors of each community that indicate the relative level of enrichment or depletion of ions within a community. We demonstrate our technique on a set of APT reconstructions of irradiated 316 stainless steel. Our method detected chromium carbide and Ni-Si-rich precipitates and located a grain boundary based on Ni and Si enrichment. Spatial correlations between communities indicated that chromium carbide precipitates were flanked by regions of Fe depletion. Our results highlight the potential of CCD in the analysis of chemical segregation in broader classes of materials, in terms of both varying synthesis methods and exposure to extreme environments.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144528751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Correlative Approach for the Assessment of Porosity in Shale Rocks by Scanning Electron Microscopy. 用扫描电镜评价页岩孔隙度的相关方法

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf020

Federico Medina, Ignacio Jausoro, María Alejandra Floridia Addato, María Jimena Rodríguez, Alberto Caneiro

{"title":"A Correlative Approach for the Assessment of Porosity in Shale Rocks by Scanning Electron Microscopy.","authors":"Federico Medina, Ignacio Jausoro, María Alejandra Floridia Addato, María Jimena Rodríguez, Alberto Caneiro","doi":"10.1093/mam/ozaf020","DOIUrl":"https://doi.org/10.1093/mam/ozaf020","url":null,"abstract":"A correlative approach to compute porosity at the nanoscale in areas as large as 3 mm × 3 mm through field emission scanning electron microscopy (FESEM) is presented. This approach overcomes the limitation of FESEM related to its representativeness on larger areas. The strategy is based on imaging an area of 3 mm × 3 mm where a set of regions of interest (ROIs) of 600 μm × 600 μm with similar mineralogy is selected and imaged. A second selection of areas of 150 μm × 150 μm inside the ROIs is performed. The three sets of images, linked through their mineralogy and organic matter, account for pore sizes within their given resolution range, providing an efficient computational method. The cumulative porosity plot, in addition of showing the contribution of the different pore sizes to the total porosity, enables the detection of fissures. The pore size distribution function allows to discuss the practical limit for counting small pores, around 30-40 nm for the samples studied in this work, under the given experimental conditions. This value is appreciably larger than the resolution of FESEM and the minimum pore size defined considering either 1 pixel/1 pore or 9 pixels/1 pore.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Histological Analysis of the Parotid and Submandibular Glands in Ovine and Caprine Species Using Histochemical, Immunohistochemical, and Stereological Methods. 用组织化学、免疫组织化学和体视学方法对绵羊和山羊腮腺和下颌骨腺的比较组织学分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf025

Diaa Massoud, Mervat A AbdRabou, Attalla F El-Kott, Kareem Morsy, Ali S AlSheri, Sally Negm, Mohamed Abumandour

{"title":"Comparative Histological Analysis of the Parotid and Submandibular Glands in Ovine and Caprine Species Using Histochemical, Immunohistochemical, and Stereological Methods.","authors":"Diaa Massoud, Mervat A AbdRabou, Attalla F El-Kott, Kareem Morsy, Ali S AlSheri, Sally Negm, Mohamed Abumandour","doi":"10.1093/mam/ozaf025","DOIUrl":"https://doi.org/10.1093/mam/ozaf025","url":null,"abstract":"This work was designed to compare the histochemical, immunohistochemical, and stereological features of the parotid and mandibular glands of sheep and goat as representative for ovine and caprine species. The parotid gland was serous in both species, while the mandibular gland was a mixed one. Mucous acini were Periodic Acid-Schiff positive and Aldehyde Fuchsin (AF) negative. Serous acini were AF positive. The parotid gland of goat was significantly larger than that of sheep; however, the mandibular gland of sheep was significantly larger than that of goat. The volume of isolated serous acini in the mandibular gland of goat was significantly higher than that of sheep. The total length of ductal system was nonsignificant in both species, but the total volume of parotid glands' ducts was significantly higher than those of mandibular glands. The parotid glands were negative for Mucin-1; however, the mandibular glands were moderately positive. Cytokeratin 7 exhibited a pronounced expression within the ductal systems of both glands. S100 proteins were expressed intensely by serous acini of glands in sheep; however, its expression was confined to the intercalated ducts and myoepithelial cells in goat. The obtained results suggest that the feeding modalities may lead to significant variations in histological and histophysiological characteristics.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf021

Diaa Massoud, Mervat A AbdRabou, Khadija Abdul Jalil Faddladdeen, Attalla F El-Kott, Mohamed M A Abumandour, Eman Kamal Khalil

{"title":"Age-related Histological and Histochemical Changes of the Uropygial Gland in the Domestic Pigeon (Columba livia).","authors":"Diaa Massoud, Mervat A AbdRabou, Khadija Abdul Jalil Faddladdeen, Attalla F El-Kott, Mohamed M A Abumandour, Eman Kamal Khalil","doi":"10.1093/mam/ozaf021","DOIUrl":"https://doi.org/10.1093/mam/ozaf021","url":null,"abstract":"The present investigation aimed to analyze the uropygial gland's (UG) histological architecture and histochemical variation in the domestic pigeon at four posthatching age stages. The small and relatively undeveloped UG was observed at 1-day-old, and then the gland started to increase in size and complexity with age to reach its full functional size in the adult. The stroma became more organized with increased collagen fiber deposition, supporting the growing gland with age. The secretory tubules were more developed in the mature and adult pigeons than in other ages. The epithelial layer had four layers: basal layer, intermediate layer, secretory layer, and degenerative layer. The histochemical analysis using Mallory's trichrome stain revealed a positive reaction with all ages. The capsule, interlobular septum, papilla areas, and CT appeared blue, and the cellular components appeared red. The staining intensity increases with age till it becomes intensely blue with well-developed collagen in adults. Herbst corpuscles were encapsulated by a connective tissue sheath and comprised nerve cells arranged along the central axon, enveloped by collagen fibers. The findings from the current study may have a potential implication in poultry science and developmental biology.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Post-hatch Development of the Adrenal Gland in Chickens (Gallus gallus domesticus): Insights From Histology, Immunohistochemistry, Transmission Electron Microscopy, and Catecholamine Level Analysis. 鸡（Gallus Gallus domesticus）肾上腺的孵化后发育：来自组织学、免疫组织化学、透射电镜和儿茶酚胺水平分析的见解。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf018

Eman Kamal Khalil, Eman M El-Beltagi, Mohamed M A Abumandour, Maha A Aljumaa, Eman Fayad, Aya H Tantawy

{"title":"Post-hatch Development of the Adrenal Gland in Chickens (Gallus gallus domesticus): Insights From Histology, Immunohistochemistry, Transmission Electron Microscopy, and Catecholamine Level Analysis.","authors":"Eman Kamal Khalil, Eman M El-Beltagi, Mohamed M A Abumandour, Maha A Aljumaa, Eman Fayad, Aya H Tantawy","doi":"10.1093/mam/ozaf018","DOIUrl":"10.1093/mam/ozaf018","url":null,"abstract":"This study is the first to describe posthatching morphological changes in chicken adrenal glands using histology, immunohistochemistry, transmission electron microscopy, and catecholamine levels. The study involved 30 chickens in 3 posthatching stages: Juvenile (1 day old), immature (2 months old), and mature (6 months old). The results showed no demarcation between cortical and medullary, with the interrenal tissue comprising most of the adrenal parenchyma. The 1-day-old chickens did not exhibit the zoning of interrenal tissue into subcapsular and inner zones, while as they aged, the proportion of medullary tissue increased. The norepinephrine-secreting cells exhibited a significantly higher number of secretory granules compared to the epinephrine-secreting cells across the different stages. The plasma levels of catecholamines increased with advancing age. Chromogranin immunoactivity increased with age during the development of the adrenal gland. It can be concluded that the adrenal gland undergoes significant structural and functional changes during various posthatching developmental periods. In general, the study provides valuable insights into the growth and maturation of the adrenal glands in posthatch chickens, potentially aiding in understanding their maturation and their role in controlling catecholamines. In conclusion, the findings suggest that optimizing stress responses in chickens could enhance poultry production, potentially leading to the development of new stress management strategies.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantifying Patterned Features on Material Surfaces Created using Ga Ion Beam in FIB-SEM. 在FIB-SEM中使用Ga离子束创建材料表面的定量化图案特征。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf001

Supriya Ghosh, K Andre Mkhoyan

{"title":"Quantifying Patterned Features on Material Surfaces Created using Ga Ion Beam in FIB-SEM.","authors":"Supriya Ghosh, K Andre Mkhoyan","doi":"10.1093/mam/ozaf001","DOIUrl":"https://doi.org/10.1093/mam/ozaf001","url":null,"abstract":"We introduced and applied a set of parameters to quantify surface modifications and pattern resolutions made by a Ga ion beam in a focused ion beam instrument using two material systems, Si and SrTiO3. A combination of top-view scanning electron microscopy and cross-sectional scanning transmission electron microscopy imaging and energy-dispersive X-ray spectroscopy was used to study the structure, composition and measure dimensions of the patterned lines. The total ion dose was identified as the key parameter governing the line characteristics, which can be controlled by the degree of overlap among adjacent spots, beam dwell time at each spot, and number of beam passes for given beam size and current. At higher ion doses (>1015 ions/cm2), the Ga ions remove part of the material in the exposed area creating \"channels\" surrounded with amorphized regions, whereas at lower ion doses only amorphization occurs, creating \"ridges\" on the wafer surface. To pattern lines with similar sizes, an order of magnitude different ion doses was required for Si and SrTiO3 indicating strong material dependence. Quantification revealed that lines as fine as 10 nm can be reproducibly patterned and characterized on the surfaces of materials.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Dynamic Reconstruction on Particle Size and Morphology in Atom Probe Tomography. 动态重建对原子探针层析成像中颗粒大小和形貌的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf006

Constantinos Hatzoglou, Paraskevas Kontis, Heena Khanchandani, Gerald Da Costa, Baptiste Gault, François Vurpillot

引用次数: 0

The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration. CXCL12/CXCR4信号通路在调控细胞迁移中的作用

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf011

Austin N Worden, Emma Grace Pittard, Matt Stern, Mark J Uline, Jay D Potts

{"title":"The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration.","authors":"Austin N Worden, Emma Grace Pittard, Matt Stern, Mark J Uline, Jay D Potts","doi":"10.1093/mam/ozaf011","DOIUrl":"10.1093/mam/ozaf011","url":null,"abstract":"We investigated the CXCL12/CXCR4 signaling pathway as a regulator of adipose-derived stem cell (ADSC) self-assembling toroidal constructs using collagen hydrogels. ADSCs formed toroid rings when cultured on hydrogel surfaces but failed to do so when mixed within the matrix. Gene expression profiling revealed significant upregulation of the CXCL12/CXCR4 pathway in toroid-forming conditions, supported by immunofluorescence studies that confirmed CXCL12 presence in toroids but not in mixed-in cultures. Early toroid formation was marked by the emergence of CXCL12 expression, correlating with cell migration. Targeted inhibition experiments identified the PI3K pathway as a critical regulator, delaying cell migration by ∼16 h, while N-Cadherin, Ras/Raf, and ERK1/2 inhibition either reduced or halted migration over extended periods. Through Western blot analysis, altered expression of α-Smooth muscle actin and focal adhesion kinase under PI3K inhibition was highlighted thus emphasizing their roles in toroid formation. Lastly, initial coculture studies with 4T1 breast cancer cells unexpectedly showed CXCL12 localization primarily in 4T1 cells within mixed toroids, suggesting modified chemotactic signaling. Our findings establish CXCL12/CXCR4 as crucial for ADSC toroid formation and reveal the pathway's complex involvement in cellular organization and migration, presenting a robust model for exploring cell-cell and cell-matrix interactions relevant to tissue engineering and cancer research.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Efficient Method for Preparing High-Quality Atom Probe Tomography Samples of Dual-Phase Ti Alloys. 制备高质量双相钛合金原子探针断层扫描样品的高效方法

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf009

Ruiqing Lu, Felix Theska, Shenglu Lu, Hao Wang, Ma Qian, Sophie Primig, Simon P Ringer, Xiaozhou Liao

引用次数: 0

3D Histological Analysis of Soft Tissues by Contrast-Enhanced X-Ray Microfocus Computed Tomography: Screening and Staining Optimization of Contrast-Enhancing Staining Agents. 对比增强x射线微聚焦计算机断层扫描软组织三维组织学分析：对比增强染色剂的筛选和染色优化。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-03-17 DOI: 10.1093/mam/ozaf013

Delia Hoffmann, Tim Balcaen, Sarah Vangrunderbeeck, Léna Puigdevall Mata, Arne Maes, Grzegorz Pyka, Laure Dumoutier, Catherine Behets, Wim De Borggraeve, Greet Kerckhofs

{"title":"3D Histological Analysis of Soft Tissues by Contrast-Enhanced X-Ray Microfocus Computed Tomography: Screening and Staining Optimization of Contrast-Enhancing Staining Agents.","authors":"Delia Hoffmann, Tim Balcaen, Sarah Vangrunderbeeck, Léna Puigdevall Mata, Arne Maes, Grzegorz Pyka, Laure Dumoutier, Catherine Behets, Wim De Borggraeve, Greet Kerckhofs","doi":"10.1093/mam/ozaf013","DOIUrl":"10.1093/mam/ozaf013","url":null,"abstract":"The gold standard for studying biological soft tissues at the microscale (i.e., histology) is tissue sectioning with subsequent colorimetric or fluorescent staining and visual inspection under the microscope. When tissue integrity must be maintained for 3D histological assessment, contrast-enhanced microfocus X-ray computed tomography (CECT) is a promising solution, but there is still a lack of staining protocol optimization of contrast-enhancing staining agents (CESAs). Therefore, in this study, mouse auricles were incubated with Hafnium-substituted Wells-Dawson polyoxometalate, cationic iodinated contrast agent, or Lugol's iodine and were imaged with high-resolution CECT. Alignment with corresponding H&E-stained sections enabled the identification and segmentation of different tissue types. Contrast differences between tissue types were increased by washing the samples after staining or by combining CESAs. Finally, we proved that the latter could be used to quantitatively assess the 3D thickness distribution of the epidermis in the ears of a mouse model of psoriasis-like dermatitis. In conclusion, CECT and bright-field microscopy are complementary and not mutually exclusive techniques for the histological assessment of biological tissues. While bright-field microscopy gives detailed information about the cellular composition of tissues, CECT provides a better insight into the spatial interrelationship of tissues and is a powerful tool for performing 3D structural quantification.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 2","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0