Microscopy and Microanalysis最新文献_第8页

Nonconventional Imaging for Viable Bacteria Detection: A Review. 用于可存活细菌检测的非常规成像：综述。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-15 DOI: 10.1093/mam/ozae100

Yilbert Gimenez, Philippe Vandenkoornhuyse

引用次数: 0

Enhancing Semantic Segmentation in High-Resolution TEM Images: A Comparative Study of Batch Normalization and Instance Normalization. 增强高分辨率 TEM 图像的语义分割：批量归一化与实例归一化的比较研究

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-15 DOI: 10.1093/mam/ozae093

Bashir Kazimi, Stefan Sandfeld

引用次数: 0

Large-Angle Rocking Beam Electron Diffraction of Large Unit Cell Crystals Using Direct Electron Detector. 使用直接电子探测器对大单元晶胞进行大角度摇摆光束电子衍射

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-02 DOI: 10.1093/mam/ozae088

Robert Busch, Hsu-Chih Ni, Yu-Tsun Shao, Jian-Min Zuo

引用次数: 0

Obtaining 3D Atomic Reconstructions from Electron Microscopy Images Using a Bayesian Genetic Algorithm: Possibilities, Insights, and Limitations. 使用贝叶斯遗传算法从电子显微镜图像中获取三维原子重构：可能性、启示和局限。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-10-02 DOI: 10.1093/mam/ozae090

Tom Stoops, Annick De Backer, Ivan Lobato, Sandra Van Aert

引用次数: 0

How Cryo-EM Revolutionized the Field of Bioenergetics. 低温电子显微镜如何彻底改变生物能学领域。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-09-19 DOI: 10.1093/mam/ozae089

Muratha Sottatipreedawong, Ahad Ali Kazmi, Irene Vercellino

{"title":"How Cryo-EM Revolutionized the Field of Bioenergetics.","authors":"Muratha Sottatipreedawong, Ahad Ali Kazmi, Irene Vercellino","doi":"10.1093/mam/ozae089","DOIUrl":"https://doi.org/10.1093/mam/ozae089","url":null,"abstract":"Ten years ago, the term \"resolution revolution\" was used for the first time to describe how cryogenic electron microscopy (cryo-EM) marked the beginning of a new era in the field of structural biology, enabling the investigation of previously unsolvable protein targets. The success of cryo-EM was recognized with the 2017 Chemistry Nobel Prize and has become a widely used method for the structural characterization of biological macromolecules, quickly catching up to x-ray crystallography. Bioenergetics is the division of biochemistry that studies the mechanisms of energy conversion in living organisms, strongly focused on the molecular machines (enzymes) that carry out these processes in cells. As bioenergetic enzymes can be arranged in complexes characterized by conformational heterogeneity/flexibility, they represent challenging targets for structural investigation by crystallography. Over the last decade, cryo-EM has therefore become a powerful tool to investigate the structure and function of bioenergetic complexes; here, we provide an overview of the main achievements enabled by the technique. We first summarize the features of cryo-EM and compare them to x-ray crystallography, and then, we present the exciting discoveries brought about by cryo-EM, particularly but not exclusively focusing on the oxidative phosphorylation system, which is a crucial energy-converting mechanism in humans.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142291233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities. 在过渡金属二卤化物单层上应用刀刃技术评估非线性显微镜模式的分辨率。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-08-21 DOI: 10.1093/mam/ozae061

Jovana Z Jelić, Marta Bukumira, Aleksa Denčevski, Ana Senkić, Livio Žužić, Borna Radatović, Nataša Vujičić, Tanja Pajić, Mihailo D Rabasović, Aleksandar J Krmpot

{"title":"Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities.","authors":"Jovana Z Jelić, Marta Bukumira, Aleksa Denčevski, Ana Senkić, Livio Žužić, Borna Radatović, Nataša Vujičić, Tanja Pajić, Mihailo D Rabasović, Aleksandar J Krmpot","doi":"10.1093/mam/ozae061","DOIUrl":"10.1093/mam/ozae061","url":null,"abstract":"We report application of the knife-edge technique at the sharp edges of WS2 and MoS2 monolayer flakes for lateral and axial resolution assessment in all three modalities of nonlinear laser scanning microscopy: two-photon excited fluorescence (TPEF), second- and third-harmonic generation (SHG, THG) imaging. This technique provides a high signal-to-noise ratio, no photobleaching effect and shows good agreement with standard resolution measurement techniques. Furthermore, we assessed both the lateral resolution in TPEF imaging modality and the axial resolution in SHG and THG imaging modality directly via the full-width at half maximum parameter of the corresponding Gaussian distribution. We comprehensively analyzed the factors influencing the resolution, such as the numerical aperture, the excitation wavelength and the refractive index of the embedding medium for the different imaging modalities. Glycerin was identified as the optimal embedding medium for achieving resolutions closest to the theoretical limit. The proposed use of WS2 and MoS2 monolayer flakes emerged as promising tools for characterization of nonlinear imaging systems.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"671-680"},"PeriodicalIF":2.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141590644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inverse Modeling of Heterogeneous Structures in Electron Probe Microanalysis. 电子探针显微分析中异质结构的逆建模。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-08-21 DOI: 10.1093/mam/ozae066

Silvia Richter, Gaurav Achuda, Philippe T Pinard, Tamme Claus, Manuel Torrilhon

{"title":"Inverse Modeling of Heterogeneous Structures in Electron Probe Microanalysis.","authors":"Silvia Richter, Gaurav Achuda, Philippe T Pinard, Tamme Claus, Manuel Torrilhon","doi":"10.1093/mam/ozae066","DOIUrl":"10.1093/mam/ozae066","url":null,"abstract":"Electron probe microanalysis (EPMA) is a powerful tool for chemical characterization of materials on a microscopic scale. However, EPMA has the drawback that its information volume has a spatial extent of some 100 nm to a few µm. With the introduction of new electron sources, i.e., Schottky Thermal Field and Cold Field Emitter, where the electron beam is focused down to a few nm, measurements can be nowadays performed on the sub-micrometer scale. The goal of the work is to reveal the chemical composition of structures smaller than the excitation volume. New strategies are presented where the acquisition is performed at different positions on the sample and as a scan across a fine structure by using one or more single beam energies. Besides the well-known Monte-Carlo simulation, a deterministic model is also used. The deterministic model is based on moment equations of the Boltzmann equation. Inverse modeling is presented for several case studies. Due to the highly complex nonlinearity of the inverse model, an ill-posed and well-posed problem is shown as well. Finally, the method is extended to reconstruct 2D structures, i.e., rectangular shaped particles, with heterogeneous composition on lateral and depth scale.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"729-740"},"PeriodicalIF":2.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Modular Cryo-Transfer Station for the Side-Entry Transmission Electron Microscope†. 为侧入透射电子显微镜开发模块化低温转移站†。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-08-21 DOI: 10.1093/mam/ozae063

Alexander Reifsnyder, Jordan A Hachtel, Andrew R Lupini, David W McComb

{"title":"Development of a Modular Cryo-Transfer Station for the Side-Entry Transmission Electron Microscope†.","authors":"Alexander Reifsnyder, Jordan A Hachtel, Andrew R Lupini, David W McComb","doi":"10.1093/mam/ozae063","DOIUrl":"10.1093/mam/ozae063","url":null,"abstract":"Cryo-transfer stations are essential tools in the field of cryo-electron microscopy, enabling the safe transfer of frozen vitreous samples between different stages of the workflow. However, existing cryo-transfer stations are typically configured for only the two most popular sample holder geometries and are not commercially available for all electron microscopes. Additionally, they are expensive and difficult to customize, which limits their accessibility and adaptability for research laboratories. Here, we present a new modular cryo-transfer station that addresses these limitations. The station is composed entirely of 3D-printed and off the shelf parts, allowing it to be reconfigured to a fit variety of microscopes and experimental protocols. We describe the design and construction of the station and report on the results of testing the cryo-transfer station, including its ability to maintain cryogenic temperatures and transfer frozen vitreous samples as demonstrated by vibrational spectroscopy. Our findings demonstrate that the cryo-transfer station performs comparably to existing commercial models, while offering greater accessibility and customizability. The design for the station is open source to encourage other groups to replicate and build on this development. We hope that this project will increase access to cryo-transfer stations for researchers in a variety of disciplines with nonstandard equipment.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"724-728"},"PeriodicalIF":2.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Methodology for Robust Multislice Ptychography. 鲁棒性多切片层析成像方法。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-08-21 DOI: 10.1093/mam/ozae055

Colin Gilgenbach, Xi Chen, James M LeBeau

引用次数: 0

TSQ Incubation Enhances Autometallographic Zinc Detection in Cultured Astrocytes. TSQ孵育可增强培养星形胶质细胞中的自金属锌检测。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2024-08-21 DOI: 10.1093/mam/ozae060

Raúl Ballestín, Josema Torres, Xavier Ponsoda

{"title":"TSQ Incubation Enhances Autometallographic Zinc Detection in Cultured Astrocytes.","authors":"Raúl Ballestín, Josema Torres, Xavier Ponsoda","doi":"10.1093/mam/ozae060","DOIUrl":"10.1093/mam/ozae060","url":null,"abstract":"Zinc is a critical ion for a large number of cellular functions. In the central nervous system, zinc ions are involved in synaptic transmission. Therefore, zinc homeostasis is essential, and cells have developed a variety of mechanisms to control cellular zinc concentration, including the zincosome formation. Alterations of free zinc levels have been associated with brain dysfunction and are present in many illnesses and syndromes. Astrocytes are implicated in the maintenance of the neuronal milleu and brain homeostasis. In this work, we have analyzed the combination of direct (TSQ) and indirect (autometallography) zinc detection methods to increase sensitivity for studying zinc uptake by rat astrocytes in vitro. Zincosome formation was visualized with the zinc fluorochrome TSQ by light microscopy. Additionally, we improved both zinc precipitation and cellular fixation methods to preserve zinc ions and make them suitable for autometallography development. Our tests pinpointed paraformaldehyde and sodium sulfide as the more adequate methods for cellular fixation and zinc precipitation, respectively. TSQ incubation and pH of the fixative were shown to be crucial for autometallography. Using this improved method, we visualized the zinc content of zincosomes at the ultrastructural level both as silver autometallographic precipitates and as electrodense sulfide-osmium zinc precipitates.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":" ","pages":"759-770"},"PeriodicalIF":2.9,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0