Microscopy and Microanalysis最新文献_第7页

The Role of Laser Pulse Energy on Data Quality and Reconstruction of Olivine Atom Probe Tomography Data. 激光脉冲能量对橄榄石原子探针层析成像数据质量和重建的影响。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf040

Dimitrios Dimitriou, Steven M Reddy, David W Saxey, Denis Fougerouse, Katy Evans, Xiao Sun, William D A Rickard

{"title":"The Role of Laser Pulse Energy on Data Quality and Reconstruction of Olivine Atom Probe Tomography Data.","authors":"Dimitrios Dimitriou, Steven M Reddy, David W Saxey, Denis Fougerouse, Katy Evans, Xiao Sun, William D A Rickard","doi":"10.1093/mam/ozaf040","DOIUrl":"https://doi.org/10.1093/mam/ozaf040","url":null,"abstract":"The study of the structure and geochemistry of olivine crystal defects is important but difficult because of their nanometer size and the analytical limitations of most techniques. Laser-assisted atom probe tomography (APT) is capable of sub-nanometer resolution, quantitative geochemical analysis and 3D reconstruction of olivine defects, but optimal analytical conditions and data reconstruction strategies have not been sufficiently studied. Here, we investigate the effect of different laser pulse energy (LPE) and crystal orientations on the quality and reconstruction parameters of APT data using specimens from two San Carlos olivine grains. Our findings show that increased LPE reduces the background noise, percentage of multiple hit events, and applied electric field, as shown by the Mg2+/Mg+ ratio, but increases the peak tails. The major element compositions show inaccuracies under all LPEs but exhibit higher consistency for higher LPEs. We determine that a LPE of 150pJ is the best compromise for optimal data quality in olivine. Using scanning electron microscopy imaging before and after APT analyses, we suggest that the Mg2+/Mg+ ratio can be used as a guide to estimate the electric field parameter and results in more accurate reconstructions.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scanning Transmission Electron Microscopy-Atom Probe Tomography Correlative Analysis for the Characterization of Solute-defect Interactions. 扫描透射电子显微镜-原子探针层析成像表征溶质缺陷相互作用的相关分析。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf039

Siwei Chen, Jonathan D Poplawsky, Steven J Zinkle, Matthew Boebinger, Yajie Zhao

{"title":"Scanning Transmission Electron Microscopy-Atom Probe Tomography Correlative Analysis for the Characterization of Solute-defect Interactions.","authors":"Siwei Chen, Jonathan D Poplawsky, Steven J Zinkle, Matthew Boebinger, Yajie Zhao","doi":"10.1093/mam/ozaf039","DOIUrl":"https://doi.org/10.1093/mam/ozaf039","url":null,"abstract":"Atom probe tomography (APT) and (scanning) transmission electron microscopy ((S)TEM) are complementary techniques that provide spatially resolved chemical and structural information at the atomic scale. In this study, we employ two different STEM/APT correlative analysis methods to investigate Cr segregation at dislocation loops in ultra-high purity Fe-Cr alloys. APT needles for the correlative analysis were extracted either from bulk material or from thinned TEM lamellae. STEM analysis was used to determine the Burgers vectors of ion-irradiation-induced dislocation loops, while APT reconstruction of the same region revealed the Cr segregation to these loops. We extended the g•b = 0 invisibility criterion of dislocation loops from TEM mode in a lamella to STEM mode in a needle-shaped specimen. STEM and APT analysis on the same needle provide straightforward correlative analysis, although it is limited by a small observation volume. In contrast, iterative STEM analysis of TEM lamellae, followed by the selective extraction of specific regions of interest for APT analysis, expands the observation area by up to 100 times but requires additional time-consuming steps for APT needle extraction from the lamellae.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quasi-ray Tracing Method for Coulomb Interaction Estimation in Charged Particle Systems. 带电粒子系统库仑相互作用估计的准射线追踪方法。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf031

Mingkang Wang, Weiming Ren, Zizhou Gong

{"title":"Quasi-ray Tracing Method for Coulomb Interaction Estimation in Charged Particle Systems.","authors":"Mingkang Wang, Weiming Ren, Zizhou Gong","doi":"10.1093/mam/ozaf031","DOIUrl":"https://doi.org/10.1093/mam/ozaf031","url":null,"abstract":"Coulomb interaction (CI) is a crucial factor contributing to the degradation of resolution in charged particle imaging systems. For example, certain inspection applications of scanning electron microscopes (SEM) require high probe current, which exacerbates resolution degradation due to CI. However, current methods for estimating CI, such as direct ray tracing and analytical approaches, struggle to balance speed and accuracy. This limitation significantly impedes the efficient optimization of SEM design parameters. In this study, we propose a quasi-ray tracing method based on perturbation theory, which simulates CI-induced trajectory displacement and the Boersch effect using a Monte Carlo ray tracing approach. Our method eliminates the need for iterative solvers employed in conventional direct ray tracing, thereby increasing simulation speed by over 105 times. Benchmarking against the direct ray tracing method demonstrates an error margin of less than 1% over a broad parameter range covering typical SEM applications. This quasi-ray tracing approach offers a fast, accurate, and general solution for CI estimation in charged particle imaging systems.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144035194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: The Role of the CXCL12/CXCR4 Signaling Pathway in Regulating Cellular Migration. 修正：CXCL12/CXCR4信号通路在调节细胞迁移中的作用。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf053

引用次数: 0

Post-Hatching Development of the Tongue in Egyptian Fayoumi Chickens (Gallus g. domesticus): New Emphasis on Age-Relational Through Scanning Electron Microscopy, Histological, and Histochemical Techniques. 埃及法尤米鸡（Gallus g. domesticus）舌头的孵化后发育：通过扫描电镜、组织学和组织化学技术对年龄关系的新强调。

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf050

Eman Kamal Khalil, Aya H Tantawy, Attalla F El-Kott, Diaa Massoud, Mohamed M A Abumandour

{"title":"Post-Hatching Development of the Tongue in Egyptian Fayoumi Chickens (Gallus g. domesticus): New Emphasis on Age-Relational Through Scanning Electron Microscopy, Histological, and Histochemical Techniques.","authors":"Eman Kamal Khalil, Aya H Tantawy, Attalla F El-Kott, Diaa Massoud, Mohamed M A Abumandour","doi":"10.1093/mam/ozaf050","DOIUrl":"10.1093/mam/ozaf050","url":null,"abstract":"The present study was the first attempt to describe age-related morphological lingual characterizations of Egyptian Fayoumi chickens at three post-hatching stages (1-day-old, 2-month-old, and 6-month-old) using SEM, histological, and histochemical techniques. There were characteristic features for each age. The papillary crest shape changed as the chickens aged: an arc-shaped crest in 1-day-olds, a \"V\" shape in 2-month-olds, and a serpentine pattern in 6-month-old chickens. The glottis was bordered by a papillary border in 1-day-old and by rostral nonpapillary and papillary portions in 2- and 6-month-olds. The apex and body carried fork-shaped filiform papillae in 1-day-olds; it had long, thick-branched papillae on the lateral tip region; the apex carried thin-branched papillae in 2-month-olds; and it had thick, long papillae in 6-month-old chickens. The rostral apical part was surrounded by membranes, two or three in 1-day-olds, four to five in 2-month-olds, and six to seven in 6-month-old chickens. These glands were located only in the free tongue and the root's second half. Histochemically, all ages had positive PAS and AB reactions, with moderate reactivity in the anterior and strong reactivity in the posterior part. Collagen fibers were seen within the connective tissue core, and glandular acini varied among ages: lower in 1-day-old, moderate in 2-month-old, and abundant in 6-month-old chickens. In conclusion, the tongue evolved to strongly adapt to the food particles available at each age stage.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Comparison Between STEM-HAADF and TEM Bright-field Mode for Imaging Resin Embedded Biological Samples. STEM-HAADF与TEM亮场模式在树脂包埋生物样品成像中的综合比较

IF 3 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf038

Kayla Lynne Haberman, Jelena Danilovic Lukovic, Snezana Kovacevic, Xinyi Zhang, Bessie Kebaara, Joseph Taube, Debora Berti, Bernd Zechmann

{"title":"Comprehensive Comparison Between STEM-HAADF and TEM Bright-field Mode for Imaging Resin Embedded Biological Samples.","authors":"Kayla Lynne Haberman, Jelena Danilovic Lukovic, Snezana Kovacevic, Xinyi Zhang, Bessie Kebaara, Joseph Taube, Debora Berti, Bernd Zechmann","doi":"10.1093/mam/ozaf038","DOIUrl":"10.1093/mam/ozaf038","url":null,"abstract":"Most investigations of resin embedded biological samples by transmission electron microscopy (TEM) have been performed in TEM bright-field mode where the electron beam transmits through the sample. Modern TEMs can also be used in scanning TEM mode (STEM) where the beam scans across the sample. The preferred detector for STEM mode is the high-angle annular dark-field (HAADF) detector. The aim of this study was to compare image quality of resin embedded biological samples such as yeast (Saccharomyces), algae (Chlorella, Haematococcus), plant leaves (Nicotiana), human cells (MCF7), and animal tissue (mouse liver and brain) between TEM bright-field and STEM-HAADF mode. Generally, images taken in STEM-HAADF mode showed better image quality in terms of contrast, brightness, and signal-to-noise ratio. Samples of sections that did not receive postcontrasting with uranyl acetate or lead citrate appeared significantly less grainy. Specifically, STEM-HAADF mode resulted in significantly better image quality of algae cells, MCF7, and liver cells that did not receive postcontrasting. Artifacts visible in TEM mode were absent in STEM-HAADF mode. Thus, we can conclude that STEM-HAADF mode has significant advantages when investigating resin embedded biological samples that have little contrast or sections that did not receive postcontrasting rendering postcontrasting of sections unnecessary.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scanning Electron Microscopy (SEM)-based Analysis of Fish Scales in the Genus Schizothorax (Teleostei: Cyprinidae) From Kashmir Himalayas. 基于扫描电子显微镜（SEM）的克什米尔喜马拉雅地区裂胸属鱼鳞分析（远骨目：鲤科）

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf052

Misba Rehman, Syed Talia Mushtaq, Tasaduq Hussain Shah, Farooz Ahmad Bhat, Adnan Abubakr, M Iqbal Jeelani

{"title":"Scanning Electron Microscopy (SEM)-based Analysis of Fish Scales in the Genus Schizothorax (Teleostei: Cyprinidae) From Kashmir Himalayas.","authors":"Misba Rehman, Syed Talia Mushtaq, Tasaduq Hussain Shah, Farooz Ahmad Bhat, Adnan Abubakr, M Iqbal Jeelani","doi":"10.1093/mam/ozaf052","DOIUrl":"https://doi.org/10.1093/mam/ozaf052","url":null,"abstract":"In this study, scanning electron microscopy (SEM) was used to investigate the microstructural scale morphology of five Schizothorax species (S. curvifrons, S. esocinus, S. labiatus, S. niger, and S. plagiostomus) from Kashmiri waters. The research aimed to improve taxonomic clarity and facilitate species differentiation within this genus. Species-specific variations in scale features revealed by SEM included length, width and shape of scales; focus area sculpture; number and width of radii; circuli number and intercircular space; length of lateral line canal and size of anterior opening of canal. Notable findings include polygonal-shaped scales in S. niger and S. plagiostomus, while circular scales were noted in S. curvifrons, S. esocinus, and S. labiatus. S. curvifrons had the largest scales, while S. plagiostomus had the smallest. The maximum number of radii was observed in S. plagiostomus, and the minimum in S. niger. Circuli were most numerous in S. esocinus and S. plagiostomus, and minimum in S. niger. Moreover, lepidonts on circuli and chromatophores on the posterior margin were absent across all species. Lateral line canal length was similar in S. esocinus, S. labiatus, and S. plagiostomus, with significant variation in S. niger and S. curvifrons. These morphological traits provide valuable insights into the differentiation of Schizothorax species and serve as a foundation for resolving taxonomic complexities within this genus. Such findings are crucial for accurate species identification, a key step in developing effective conservation strategies for these endemic fishes of the Kashmir Valley.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid-Acquisition Fluctuation Electron Microscopy: Decoherence and the Dominant Role of Noise. 快速采集波动电子显微镜：退相干和噪声的主导作用。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf049

Armin Zjajo, Hongchu Du, Rafal E Dunin-Borkowski, J Murray Gibson, Aram Rezikyan, Michael M J Treacy

引用次数: 0

Rapid-Acquisition Fluctuation Electron Microscopy: Decoherence and the Dominant Role of Noise. 快速采集波动电子显微镜：退相干和噪声的主导作用。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf049

Armin Zjajo, Hongchu Du, Rafal E Dunin-Borkowski, J Murray Gibson, Aram Rezikyan, Michael M J Treacy

引用次数: 0

On the Formation of Quaternary Particles, Based on Lead-Barium-Antimony + Tin, Considered Characteristic of GSRs. 基于铅-钡-锑+锡的四元粒子的形成，认为是gsr的特征。

IF 2.9 4区工程技术

Microscopy and Microanalysis Pub Date : 2025-05-09 DOI: 10.1093/mam/ozaf028

Felice Nunziata, Claudio Gentile, Marco Romeo, Giancarlo Palumbo, Gabriele Rotter

{"title":"On the Formation of Quaternary Particles, Based on Lead-Barium-Antimony + Tin, Considered Characteristic of GSRs.","authors":"Felice Nunziata, Claudio Gentile, Marco Romeo, Giancarlo Palumbo, Gabriele Rotter","doi":"10.1093/mam/ozaf028","DOIUrl":"https://doi.org/10.1093/mam/ozaf028","url":null,"abstract":"The presence of tin in gunshot residue (GSR) particles from ammunitions used in short and long rifled firearms is well known and widely documented. Tin does not originate from the primer mixture itself but is typically linked to a thin disc-shaped foil placed over mercury fulminate-based primers to prevent embrittlement of the cartridge case and primer cap. However, this practice has not been previously reported or observed in hunting ammunitions. We designed an experimental protocol to investigate the formation of quaternary GSR particles containing Pb-Ba-Sb-Sn, following shots fired from a double-barrel shotgun using modified hunting ammunitions. These ammunitions were loaded with multiple pellets and included a thin tin foil over the primer's flash hole. Our results confirm that the tin foil is directly responsible for the origin and composition of the quaternary GSR particles detected on the collected stubs. Additionally, the hunting ammunitions market has recently seen increased production of environmentally friendly ammunitions, including shot pellets made of tin, tin alloys, or coated with tin. Our findings provide a foundation for further experimental studies into GSR from such eco-friendly ammunitions, especially in forensic investigations involving tin-containing pellets.","PeriodicalId":18625,"journal":{"name":"Microscopy and Microanalysis","volume":"31 3","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0