Microbial pathogenesis最新文献

{"title":"Phyto-molecules show potentials to combat drug-resistance in bacterial cell membranes.","authors":"Adekunle Rowaiye, Gordon C Ibeanu, Doofan Bur, Sandra Nnadi, Ugonna Morikwe, Akwoba Joseph Ogugua, Chinwe Uzoma Chukwudi","doi":"10.1016/j.micpath.2025.107723","DOIUrl":"10.1016/j.micpath.2025.107723","url":null,"abstract":"<p><p>The global rise in antibiotic resistance and the emergence of infectious diseases have intensified the need for novel antimicrobial therapies. As a result, there is a growing demand to validate the ethnomedicinal claims that plant extracts possess antibacterial properties. This validation requires the characterization of specific phytoconstituents, including anti-infective compounds and antimicrobial peptides. This study explores the progress made in identifying and producing anti-infectives derived from plants, with a focus on their mechanisms of action, current applications, and future potentials. One key area of investigation is the therapeutic potential of phyto-molecules, that target bacterial cell membranes. These molecules which include phenols, alkaloids, terpenoids, saponins, and peptides, have shown significant ability to disrupt bacterial cell membranes through various molecular mechanisms. By impairing membrane integrity, inhibiting efflux pumps, and altering membrane permeability, phyto-molecules offer a novel strategy for combating drug-resistant bacterial strains. This disruption not only enhances the efficacy of conventional antibiotics but also provides standalone antimicrobial activity. In conclusion, phyto-molecules represent a promising solution to overcoming antibiotic resistance, with their ability to target structural and functional components of bacterial membranes offering new pathways for therapeutic development. However, further research is needed to assess the comparative effectiveness and safety of these plant-based molecules in relation to traditional membrane-disrupting antibiotics.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107723"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of 2-(4-aminopiperidin-4-yl)acetic acid (β^3,3-Pip) derivatives and its peptides conjugated with lauric acid through the side chain against methicillin-resistant Staphylococcus aureus (MRSA).","authors":"Rubina Chowdhary, Arti Rathore, Aminur Rahman Sarkar, Jyoti Kumari, Rakshit Manhas, Shifa Firdous, Avisek Mahapa, Rajkishor Rai","doi":"10.1016/j.micpath.2025.107693","DOIUrl":"10.1016/j.micpath.2025.107693","url":null,"abstract":"<p><p>The present work describes the synthesis, characterization, and antibacterial efficacy of cationic β-amino acid derivatives and peptides, H₂N-β^3,3-Pip(LA)-PEA, P1; and H₂N-β^3,3-Pip (^ULA)-PEA, P2; H₂N-β^3,3-Pip(LA)-β^2,2-Ac₆c-PEA, P3; and H₂N- β^3,3-Pip(^ULA)- β^2,2-Ac₆c-PEA, P4. The compounds P1-P4 were evaluated against the WHO priority multidrug-resistant (MDR) ESKAPE panel pathogens. P2 and P4 exhibited potent activity with MIC values ranging from 3.1 μM to 6.2 μM against MDR pathogens. Further, the kill-kinetics assay demonstrated that P2 and P4 eliminate MRSA in a concentration and time-dependent manner. P2 and P4 also showed the MRSA biofilm prevention and disruption of preformed biofilm. The SEM images and PI permeability assays confirmed the bacterial killing by P2 and P4 through membrane disruption, highlighting their strong bactericidal activity. Additionally, the very low hemolytic and cytotoxic activity of peptides indicate these compounds as promising candidates for further investigation. Subsequently, the compounds P2 and P4 showed synergistic effects with vancomycin. Altogether, the present study highlights the potential of short cationic β-amino acid derivatives and peptides conjugated with lauric acid through the side chain as novel antibacterial agents for combating antimicrobial resistance (AMR).</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107693"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing of eucalyptol nanoemulsion drug delivery system loaded with amphotericin B for Candida albicans biofilm control: in vitro and in vivo assessment using the Galleria mellonella model.","authors":"Mahyar Keymaram, Ensieh Lotfali, Marziyeh Mousazadeh, Maryam Nikkhah, Omid Raiesi, Mohammad Hossein Yadegari","doi":"10.1016/j.micpath.2025.107719","DOIUrl":"10.1016/j.micpath.2025.107719","url":null,"abstract":"<p><strong>Objective: </strong>(s): Candida species can form biofilm in various clinical settings, making them challenging to treat due to their resistance to antifungal drugs. The present study aimed to evaluate the effects of eucalyptol/amphotericin B nanoemulsion (NEA) on Candida albicans (C. albicans) biofilm.</p><p><strong>Materials and methods: </strong>In this study, NEA was synthesized and its capacity to combat biofilms generated by four clinical isolates and a reference strain of C. albicans was investigated according to CLSI M27-A3 guidelines. The NEA was prepared using eucalyptol oil, Tween 80, and ethanol, and characterized by measuring particle size, Z-potential, and encapsulation efficiency (EE %). The cytotoxicity study was performed on myeloblast KG1 cells and human RBCs. The gene assessment and molecular docking were conducted by Real-Time PCR and AutoDock Vina software, respectively. The in vivo stage was conducted on Galleria mellonella (G. mellonella) larvae, and ultimately, histopathological analysis was executed using H and E Staining.</p><p><strong>Results: </strong>Based on the results obtained, the NEA exhibited EE of 80 % for Amphotericin B (AmB), displaying an average size of 39 nm and zeta potential of -2 mV. Minimum inhibitory concentration (MIC) and minimum biofilm inhibitory concentration (MBIC) revealed more efficiency to inhibit biofilms (MIC Geomean = 4.25 μg/mL, MBIC Geomean = 19.58 μg/mL) compared to AmB (MIC Geomean = 17.05 μg/mL, MBIC Geomean = 59.37 μg/mL). While ALS1 and HWP1 gene expression remained unchanged, molecular docking suggested the binding of eucalyptol to ALS1 and HWP1 proteins. The results of FE-SEM demonstrated the biofilm destruction through yeast bursting and cytoplasm leakage. The NEA, in comparison to AmB, decreased melanization in tissue sections and also enhanced the survival of G. melonella larvae.</p><p><strong>Conclusion: </strong>The NEA exhibited superior biocompatibility toward the KG1 myeloblasts cell line and RBCs cells additionally enhances the survival rate of G. mellonella larvae compared to AmB. Overall, NEA proved an efficient nano-formulated product against C. albicans biofilms.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107719"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization studies on the 05SSU0969 gene of Streptococcus suis serotype 2 in adhesion, anti-phagocytosis, and virulence in animal.","authors":"Yaya Pian, Yaxuan Wang","doi":"10.1016/j.micpath.2025.107739","DOIUrl":"10.1016/j.micpath.2025.107739","url":null,"abstract":"<p><strong>Objective: </strong>Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, however, the pathogenesis of SS2 remains unclear. Our objective was to study the role of the type IV secretion system (T4SS) 05SSU0969 gene in SS2 virulence.</p><p><strong>Methods: </strong>To assess the function of 05SSU0969 in SS2 virulence, the wild-type strain 05ZYH33 served as the parent strain. Using homologous recombination technology, the mutant strain Δ0969 and the complemented strain CΔ0969 were generated. The biological properties of these strains were evaluated through growth curve analysis, cultivation characteristics, cell adhesion assays, blood survival assays, and pathogenicity tests in CD1 mice and piglets.</p><p><strong>Results: </strong>Growth curve analysis revealed no significant differences among 05ZYH33, Δ0969, and CΔ0969. Although Δ0969 exhibited similar cultivation characteristics to 05ZYH33 on sheep blood agar, its adhesion to A549, Hep-2, and HBMEC cells was significantly impaired. Additionally, Δ0969 demonstrated markedly reduced blood survival and attenuated virulence in both mice and piglets models.</p><p><strong>Conclusions: </strong>These findings indicate that deletion of 05SSU0969 gene attenuates SS2 resistance and virulence in CD1 mice and piglets. However, the precise mechanism by which 05SSU0969 gene influences adhesion to A549, Hep-2, and HBMEC cells requires further investigation.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107739"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Gut microbiota promotes cholesterol gallstone formation through the gut-metabolism-gene axis\" [Microb. Pathogen. 203 (2025) 107446].","authors":"Wei Wang, Kai Zhang, Kun Zhang, Rui Wu, Yu Tang, Yuliang Li","doi":"10.1016/j.micpath.2025.107627","DOIUrl":"10.1016/j.micpath.2025.107627","url":null,"abstract":"","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107627"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Green synthesized antimicrobial peptides and nanoparticles from Phoenix dactylifera: Evaluation of anti-biofilm, anti-pathogenic and anti-diabetic activities\" [Microbial Pathog. 205 (2025) 107700].","authors":"Parvaze Ahmad Wani, Tanveer A Wani, Olawale-Success Olajumoke Oluwagbemisola, Reem Naseer Alotaibi, Sanusi Jadesola Fawzhia, Seema Zargar, Bilal Ahmed","doi":"10.1016/j.micpath.2025.107756","DOIUrl":"10.1016/j.micpath.2025.107756","url":null,"abstract":"","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107756"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Luteolin: A novel approach to fight bacterial infection\" [Microb. Pathog. 204 (2025) 107519].","authors":"Maria do Socorro Dos Santos Chagas, Carla Junqueira Moragas Tellis, Adriana R Silva, Maria Alice Dos Santos Mascarenhas Brito, Anderson Junger Teodoro, Monique de Barros Elias, Stela Regina Ferrarini, Maria Dutra Behrens, Cassiano F Gonçalves-de-Albuquerque","doi":"10.1016/j.micpath.2025.107743","DOIUrl":"10.1016/j.micpath.2025.107743","url":null,"abstract":"","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107743"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Vitamin D receptor gene polymorphism and Vitamin D status to explore as a risk factor in Visceral Leishmaniasis and Post Kala Azar Dermal Leishmaniasis patients in endemic regions of Bihar.","authors":"Mehar Darukhshan Kalim, Sachidananda Behera, Niyamat Ali Siddiqui, Krishna Pandey, Vahab Ali","doi":"10.1016/j.micpath.2025.107706","DOIUrl":"10.1016/j.micpath.2025.107706","url":null,"abstract":"<p><p>The role of vitamin D and its receptors (VDR) in PKDL and VL remains uncharacterized at genetic level. This study aimed to explore the single nucleotide polymorphism of VDR gene (rs1544410, rs7975232, rs731236) in association with PKDL and VL infection. A significant difference was observed among VL and PKDL patients versus healthy group (p<0.0001) for Bsm1 polymorphism. The comparison of VL and PKDL patients versus healthy control of VDR genotypes AA and GA were found highly significant (p<0.0001). The risk for developing disease was 7.03 and 4.98 times greater in AA, while 2.49 and 2.97 times greater in GA in case of VL and PKDL patients, respectively. There was no statistical significant difference between VL and PKDL patients in VDR gene polymorphisms and allelic frequency (p>0.05). The RNA expression profile of VDR gene and CYP27B1 gene were upregulated in pretreated PKDL (1.4-1.6 fold), but in VL patients, CYP27B1 (2-3 folds) and VDR (1.5 fold) expression were down regulated. Vitamin D levels in VL patients were significantly lower (22.41 ± 10.57 ng/ml), than PKDL patients (42.19 ± 10.84 ng/ml) (p<0.01). However, no significant difference was observed in relation to the genetic data with age and sex for VL (p = 0.622) and PKDL (p = 0.786), clinical observations along with anthropometric indices. The Bsm1 gene polymorphisms may contribute in VL and PKDL infection as a risk factor.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107706"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and therapeutic effects of vancomycin-resistant Staphylococcus aureus bacteriocin (VRSAcin) in the treatment of VRSA skin infection in mice.","authors":"Ahmed Qassim Al-Awadi, Mais Emad Ahmed, Mohammad Y Alfaifi, Ali A Shati, Serag Eldin I Elbehairi, Mohammed Aufy, Ahmed M Hussein","doi":"10.1016/j.micpath.2025.107729","DOIUrl":"10.1016/j.micpath.2025.107729","url":null,"abstract":"<p><p>Vancomycin Staphylococcus aureus (VRSA) is a strain of S. aureus that is considered the main cause of bacterial skin and soft tissue infections. It has acquired resistance to vancomycin and represents a therapeutic challenge. The current study aimed to compare the possible therapeutic effects of VRSA bacteriocin (VRSAcin) on the treatment of skin infection in mice with those of an antibiotic (linezolid). The results showed that of the fifty swabs obtained from human skin wounds. One isolate was selected for VRSAcin extraction depending on its antibiotic resistance using an antibiotic susceptibility test.An agar well diffusion test was used to determine bacteriocin's antibacterial activity, as well as its a minimum inhibitory concentration, minimum bactericidal concentration, and antibiofilm efficiency against gram-positive and gram-negative bacteria that were resistant to many medicines. The freshly developed antibacterial substance VRSAcin shows promise. Bacteriocin from VRSA was extracted and studied the optimal conditions for the Production following Purification of bacteriocin by ammonium sulfate precipitation followed by cation-exchange chromatography. The molecular weight of bacteriocin about (29 kDa) were determined by Sodium Dodecyl Sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The typical conditions for the production of VRSAcin include a pH of 7 and a temperature of 37 °C for 48 h. In mice, VRSA-contaminated wounds revealed severe tissue distraction and inflammation that extended to the hypodermis, while VRSA-treated skin showed mild changes and localized lesions to the epidermis and upper dermis. The skin of linezolid ointment-treated mice showed moderate to severe changes. In conclusion, VRSA strain infections in human burned skin were more common than expected. In vivo studies in mice indicated that wounded skin infected with VRSA can be treated with VRSAcin as an antibacterial agent that promotes healing processes with obvious superiority to linezolid ointment. As a result, the VRSA develops bacteriocins that are appropriate for regulating AMR, Gram-positive and Gram-negative bacteria, and may be useful in wound dressings.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107729"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum sensing mediated attenuation of biofilm formation and virulence traits in Staphylococcus aureus by trigonelline.","authors":"Amiya Kar, Samir Kumar Mukherjee, Sk Tofajjen Hossain","doi":"10.1016/j.micpath.2025.107731","DOIUrl":"10.1016/j.micpath.2025.107731","url":null,"abstract":"<p><p>Pathogenesis of Staphylococcus aureus is largely associated with its biofilm formation, that protects the cells from host immune system and antimicrobial threats. Considering the concern over the emergence of antimicrobial resistant S. aureus strains, this study was aimed to explore an effective alternative therapeutant. Trigonelline, an alkaloid, was evaluated for its antibiofilm and antivirulence activities against S. aureus. Trigonelline efficiently inhibited and eradicated biofilm, and abled to decrease the production of protease and hemolysin, the major virulence factors of S. aureus. Inhibition of biofilm formation and eradication of mature biofilm on the catheter surface suggested its potentiality in clinical application. The observed reduction in biofilm formation and virulence factor production following trigonelline treatment may be attributed to its ability to alter the expression of key regulatory genes such as agrA, sarA, saeR, arlR, icaR, and sigB, which control quorum sensing network and biofilm development. Additionally, molecular docking analysis revealed a substantial binding affinity of trigonelline to these regulatory proteins, further supporting its possible inhibitory mechanism. Thus, trigonelline might be a promising alternative chemical lead to manage biofilm-associated bacterial infections caused by S. aureus.</p>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":" ","pages":"107731"},"PeriodicalIF":3.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}