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Development and immunogenicity evaluation of bivalent virus-like particles against bovine nebovirus and bovine norovirus in mice

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-10-17 DOI:10.1016/j.micpath.2025.108118

Chunsai He , Lu Ding , Chenxi Zhu , Jiacheng Yang , Taoyun Chen , Lan Lan , Ni Qing , Chanqing Yu , Bin Zhang

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引用次数: 0

Abstract

Bovine Nebovirus (BNeV) and Bovine Norovirus (BNoV) are major causes of viral diarrhea in calves, leading to significant economic losses. Currently, no commercial vaccines exist for these pathogens. Here, we developed bivalent virus-like particles (VLPs) co-expressing BNeV and BNoV VP1 proteins using a baculovirus-insect cell system and evaluated their immunogenicity in mice. Both monovalent (BNoV or BNeV VLPs) and bivalent VLPs induced peak IgG titers by day 14 post-booster, with the bivalent VLPs triggering faster antibody production. Histo-blood group antigen (HBGA) blocking assays (BT50) showed that bivalent VLPs induced higher blocking antibody titers, peaking at day 14 and remaining elevated at day 28. Additionally, bivalent VLPs enhanced IFN-γ secretion and increased CD3⁺CD8⁺ T-cell proportions. These findings demonstrate that BNeV-BNoV-VP1 bivalent VLPs effectively stimulate cellular immunity and blocking antibodies, supporting their potential as vaccines against BNeV and BNoV.

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二价病毒样颗粒在小鼠体内抗牛nebovirus和牛norovirus的制备和免疫原性评价

牛Nebovirus （BNeV）和牛诺如病毒（BNoV）是犊牛病毒性腹泻的主要原因，造成重大经济损失。目前，还没有针对这些病原体的商业疫苗。在此，我们利用杆状病毒-昆虫细胞系统构建了双价病毒样颗粒（VLPs），共表达BNeV和BNoV VP1蛋白，并在小鼠中评估了它们的免疫原性。单价VLPs （BNoV或BNeV VLPs）和二价VLPs在增强后第14天诱导IgG滴度达到峰值，其中二价VLPs触发抗体产生更快。组织血型抗原（HBGA）阻断试验（BT50）显示，二价VLPs诱导更高的阻断抗体滴度，在第14天达到峰值，在第28天保持升高。此外，二价VLPs增强IFN-γ分泌，增加CD3+CD8+ t细胞比例。这些发现表明，BNeV-BNoV- vp1二价VLPs可有效刺激细胞免疫并阻断抗体，支持其作为BNeV和BNoV疫苗的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)

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