Proteomic analysis of the response of Beauveria bassiana to HY60, an antifungal peptide from Pseudomonas aeruginosa of Blattella germanica

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-10-20 DOI:10.1016/j.micpath.2025.108122

Xiaoyan Wu , Xingyu Chen , Chenglong Yang , Zipeng Lin , Wenxiao Ma , Jianzheng Wang , Rong Huang , Fan Zhang

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Abstract

The antibacterial peptide HY60, isolated and purified from the gut bacterium Pseudomonas aeruginosa BGf-2 of Blattella germanica, can significantly inhibit the growth and reproduction of Beauveria bassiana in previous experiments, but the specific mechanism remains unclear. This study aimed to investigate the antimicrobial mechanism of HY60 against B. bassiana. First, both BGf-2 suspension and HY60 demonstrated significant suppression of B. bassiana proliferation. Electron microscopy further showed HY60-induced morphological aberrations including surface flocculation and vacuolization. Label-Free analysis was first used to reveal that HY60 affects key processes such as protein synthesis, oxidative stress response, energy metabolism, and cell wall biosynthesis. Western blot validation confirmed the HY60 downregulated the expression of AATM and GFAT1, consistent with proteomic results. These findings preliminarily reveal the mechanism of antimicrobial peptide HY60 against B. bassiana, providing valuable candidate targets for future development of innovative biocontrol strategies. Additionally, this study offers insights into the development of B. germanica biopesticides and lays a foundation for the research and development of mycotic drugs for beneficial economic insects.

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球孢白僵菌对德国小蠊铜绿假单胞菌抗真菌肽HY60反应的蛋白质组学分析

从德国小蠊肠道细菌铜绿假单胞菌BGf-2中分离纯化的抗菌肽HY60，在前期实验中可显著抑制球孢白僵菌的生长和繁殖，但具体机制尚不清楚。本研究旨在探讨HY60对球孢白僵菌的抑菌机制。首先，BGf-2悬浮液和HY60均能显著抑制球孢白僵菌的增殖。电镜进一步显示了hy60诱导的形态畸变，包括表面絮凝和液泡化。Label-Free分析首次揭示了HY60影响蛋白质合成、氧化应激反应、能量代谢和细胞壁生物合成等关键过程。Western blot验证证实，HY60下调了AATM和GFAT1的表达，与蛋白组学结果一致。这些发现初步揭示了抗菌肽HY60对球孢白僵菌的作用机制，为今后开发创新生物防治策略提供了有价值的候选靶点。此外，该研究为德国小蠊生物农药的开发提供了新的思路，为有益经济昆虫真菌药物的研究和开发奠定了基础。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)