MicrobiologyOpen最新文献_第3页

Macroscopic Physical Properties and Nutrient Content in the Fermentation of Pangasius Waste by Single Cultures and Microbial Consortiums and Their Potential for Feed 单菌种和菌群发酵巴沙鱼废弃物的宏观物理特性、营养成分及其饲料潜力

IF 4.6 3区生物学

MicrobiologyOpen Pub Date : 2025-08-01 DOI: 10.1002/mbo3.70040

Abun Abun, Kiki Haetami, Denny Rusmana, Rahmad Fany Ramdhan

{"title":"Macroscopic Physical Properties and Nutrient Content in the Fermentation of Pangasius Waste by Single Cultures and Microbial Consortiums and Their Potential for Feed","authors":"Abun Abun, Kiki Haetami, Denny Rusmana, Rahmad Fany Ramdhan","doi":"10.1002/mbo3.70040","DOIUrl":"https://doi.org/10.1002/mbo3.70040","url":null,"abstract":"Fish waste processing through biological technology using fermentation microbes is an important concern in handling fishery waste, because fermentation can decompose organic waste materials into useful products and reduce pollutant levels. The purpose of the study was to select three types of microbes based on macroscopic properties and nutrient content of fermented pangasius waste (FPW). In the first stage, the fermentation of pangasius waste with eight species of microbes (three of bacteria, three of fungi, and two of yeast) is cultured in a single fermentation solid-state medium. Each type of microbe that produces the highest number of colonies and nutrient content of FPW products in a single culture is then selected and used for consortium culture. The study used the nested, completely randomized design method, and the data were analyzed with ANOVA and Duncan's multiple range test. The results of single cultures of Pseudomonas aeruginosa (Pa), Rhizopus microsporus (Rm), and Yarrowia lipolytica (Yl) produced the best FPW products at 4 days of fermentation. The results of a consortium P. aeruginosa, R. microsporus, and Y. lipolytica (Pa + Rm + Yl) at a dose of 15% and fermentation for 4 days produced the best macroscopic physical properties, and its effect on nutrient content, obtained at a dose of 15% and fermentation for 4 days (protein 52.02%, fat 29.24%, and crude fiber 2.19%). The conclusion was that the consortium of three types of microbes (Pa + Rm + Yl) was more effective in treating FPW. FPW products are recommended as feed ingredients as a source of protein for poultry.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota-Host Interactions: Exploring Their Dynamics and Contributions to Human Diseases. 微生物-宿主相互作用：探索它们的动力学和对人类疾病的贡献。

IF 4.6 3区生物学

MicrobiologyOpen Pub Date : 2025-08-01 DOI: 10.1002/mbo3.70043

Siau Wui Chin, Zheng Yao Low, Wei Qi Tan, Adzzie Shazleen Azman

{"title":"Microbiota-Host Interactions: Exploring Their Dynamics and Contributions to Human Diseases.","authors":"Siau Wui Chin, Zheng Yao Low, Wei Qi Tan, Adzzie Shazleen Azman","doi":"10.1002/mbo3.70043","DOIUrl":"10.1002/mbo3.70043","url":null,"abstract":"Dysbiosis is the imbalance of bacterial composition, which would otherwise change the human host's metabolic activities and usual microbiota distribution. The outcomes would be as clear as day: losing beneficial bacteria in exchange for the overgrowth of potentially pathogenic bacteria, leading to diseases. It is crucial to unravel the dynamic roles of bacteria in maintaining human health to prevent and alleviate the said dysbiosis. To date, diet, lifestyle, age, and chemical exposures were cited as the leading cause of bacterial dysbiosis atop of genetic factors. This review aims to shed light on how bacterial interplays in maintaining human health and how bacteria-bacteria interaction may play a part in the surge of antimicrobial resistance. The intricate relationship of bacteria dynamics in the gut, skin and oral was detailed to understand how bacteria dysbiosis causes diseases such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), acne vulgaris (AV), atopic dermatitis (AD), periodontitis and dental caries. Besides that, current interventions and limitations of therapeutic prospects entailing the growing concepts of rebiosis, including probiotics, prebiotics, synbiotics, microbiota transplantation, and the evolving phage therapy, were also discussed to breathe new life into the development of novel therapeutics against dysbiosis.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":"e70043"},"PeriodicalIF":4.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12329004/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Colistin Susceptibility in Carbapenem-Resistant Acinetobacter baumannii Isolates Using Broth Microdilution, MICRONAUT-MIC-Strip, and VITEK MS. 采用微量肉汤稀释、微量检测和VITEK质谱法评价耐碳青霉烯鲍曼不动杆菌对粘菌素的敏感性。

IF 4.6 3区生物学

MicrobiologyOpen Pub Date : 2025-08-01 DOI: 10.1002/mbo3.70046

Fatih Mehmet Akıllı, Arzu İlki

{"title":"Evaluation of Colistin Susceptibility in Carbapenem-Resistant Acinetobacter baumannii Isolates Using Broth Microdilution, MICRONAUT-MIC-Strip, and VITEK MS.","authors":"Fatih Mehmet Akıllı, Arzu İlki","doi":"10.1002/mbo3.70046","DOIUrl":"10.1002/mbo3.70046","url":null,"abstract":"We aimed to analyze the colistin susceptibility using broth microdilution (BMD) and commercially available MICRONAUT-MIC-Strip (MMS) test and VITEK MS in carbapenem-resistant Acinetobacter baumannii (CRAB) isolates. Colistin susceptibility of 194 CRAB isolated from various clinical specimens between December 2020 and 2022 was determined by BMD and commercial MMS method. Minimum inhibitory concentrations of the commercial method were compared with the standard BMD. In the second part of the study, colistin susceptibility of the isolates was tested using the MALDIxin method, which detects modified lipid A, by VITEK MS, MALDI-TOF MS (bioMérieux, France). The presence of mcr-1-5 genes in resistant isolates was investigated using in-house multiplex polymerase chain reaction. Of these, 23 (11.8%) were found to be colistin resistant, whereas 171 (88.2%) isolates were susceptible. MMS categorical agreement was found to be 98.9% and essential agreement was 96.3%. The major error was found to be 1.03%, whereas a very major error was not detected. The MALDIxin test developed according to VITEK MS did not detect mutations responsible for lipopolysaccharide-induced colistin resistance in our isolates (expected peak at 1935-2033 m/z). The mcr-1-5 genes were not detected in our isolates. The MMS test is a reliable alternative method for the detection of colistin susceptibility in CRAB. Colistin resistance of the isolates used in our study was not associated with lipid A modification. Alternative mechanisms, such as efflux pumps, are a possibility. To our knowledge, this is the first VITEK MS-based method that enables rapid detection of colistin resistance in negative ion mode, and further studies are needed to determine colistin resistance in this way.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":"e70046"},"PeriodicalIF":4.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12332535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Centrins and Their Posttranslational Modification Modulate the Cell Cycle of Giardia lamblia 两种中心蛋白及其翻译后修饰调控贾第鞭毛虫的细胞周期

IF 4.6 3区生物学

MicrobiologyOpen Pub Date : 2025-07-29 DOI: 10.1002/mbo3.70038

Hye Rim Yeo, Mee Young Shin, Juri Kim, Soon-Jung Park

{"title":"Two Centrins and Their Posttranslational Modification Modulate the Cell Cycle of Giardia lamblia","authors":"Hye Rim Yeo, Mee Young Shin, Juri Kim, Soon-Jung Park","doi":"10.1002/mbo3.70038","DOIUrl":"https://doi.org/10.1002/mbo3.70038","url":null,"abstract":"Centrins, Ca2+-binding proteins conserved in eukaryotes, are the key components of the microtubule-organizing center. Giardia lamblia possesses two centrins (GL50803_6744: centrin 1; GL50803_104685: centrin 2) localized in the basal bodies during cell division. G. lamblia centrin 2 (Glcent2) is also found in the nuclei of interphase Giardia, with its N-terminal half being necessary for this localization. Morpholino-mediated knockdown of Glcents resulted in abnormal nuclear positioning and cytokinesis, causing cell malformations, including ventral discs and flagella defects. Small ubiquitin-like modifier (SUMO)ylation is a posttranslational modification, which modulates several cellular processes. Here, we demonstrated that Glcents are substrates of SUMO through in vitro SUMOylation and immunoprecipitation experiments. Additionally, treatment of Giardia with ginkgolic acid, which inhibits the E1 enzyme of the SUMO pathway, and CRISPRi-mediated inhibition of G. lamblia Ubc9, the E2 conjugation enzyme involved in SUMOylation, resulted in defects in the localization of Glcents. Blocking SUMOylation resulted in the arrest of Giardia cells and conformational changes, including alterations in the ventral disc shape, posterolateral flanges, and peripheral vesicles. Taken together, we demonstrated that Glcents function in Giardia cell cycle progression and morphogenesis, with the activity of both Glcents being modulated by SUMOylation.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70038","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on Antibacterial Activity of Water Extract of Galla chinensis Against Carbapenemase-Producing Enterobacteriaceae 五倍子水提物对产碳青霉烯酶肠杆菌的抑菌活性研究

IF 3.9 3区生物学

MicrobiologyOpen Pub Date : 2025-07-28 DOI: 10.1002/mbo3.70041

MinHui Miao, Jiayao Lin, Jie Zhu, Hong Du, Di Mei, AnQi Gu, BeiNa Hu, HaiQiang Jiang

{"title":"Study on Antibacterial Activity of Water Extract of Galla chinensis Against Carbapenemase-Producing Enterobacteriaceae","authors":"MinHui Miao, Jiayao Lin, Jie Zhu, Hong Du, Di Mei, AnQi Gu, BeiNa Hu, HaiQiang Jiang","doi":"10.1002/mbo3.70041","DOIUrl":"https://doi.org/10.1002/mbo3.70041","url":null,"abstract":"Carbapenemase-producing Enterobacteriaceae (CPE) represents a significant global public health concern, largely driven by factors including misuse of antibiotics and their inappropriate use in livestock. This study systematically evaluated the minimum inhibitory concentration (MIC) of Galla chinensis (GC) water extract against a range of CPE strains, aiming to assess its potential therapeutic application and efficacy against multidrug-resistant bacteria (MDR). MIC values for carbapenemase with different plasmids spanned between 3.9 and 500 mg/mL, while readings of 62.55–500 mg/L demonstrated no colonies. The growth curve analysis demonstrated that the GC extract significantly inhibited bacterial growth at both 1/4 and 1/2 MIC doses, completely inhibiting bacterial growth at 1 MIC (p < 0.01). Furthermore, the effect of 1/4 MIC was also statistically different; however, it may not provide a meaningful inhibitory effect. qRT-PCR analysis revealed an increase in the expression of carbapenemase genes, including pkPC2_InFII, pNDM5_IncX3, pIMP4_IncN, and pOXA48_IncX3 subsequent to GC extract treatment at 1/2 MIC. Transcriptomic analysis further revealed differential gene expression associated with bacterial resistance mechanisms. Consequently, the GC extract demonstrated pronounced antibacterial efficacy against CPE, exhibiting a significant ability to inhibit their growth and minimize the activity of vital resistance genes.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Structure–Activity Relationship Modeling and Molecular Docking Studies of TgCDPK1 Inhibitors in Toxoplasma gondii 刚地弓形虫TgCDPK1抑制剂定量构效关系建模及分子对接研究

IF 3.9 3区生物学

MicrobiologyOpen Pub Date : 2025-07-28 DOI: 10.1002/mbo3.70039

Sara Lesani, Mehdi Tavalla, Gilda Eslami, Mohammad J. Boozhmehrani

{"title":"Quantitative Structure–Activity Relationship Modeling and Molecular Docking Studies of TgCDPK1 Inhibitors in Toxoplasma gondii","authors":"Sara Lesani, Mehdi Tavalla, Gilda Eslami, Mohammad J. Boozhmehrani","doi":"10.1002/mbo3.70039","DOIUrl":"https://doi.org/10.1002/mbo3.70039","url":null,"abstract":"Toxoplasma gondii is a globally prevalent protozoan parasite responsible for severe health complications, particularly in immunocompromised individuals and during congenital infections. Existing treatments are limited by suboptimal efficacy and significant side effects, highlighting the urgent need for novel therapeutic strategies. Calcium-dependent protein kinase 1 (TgCDPK1) has emerged as a promising drug target due to its critical role in T. gondii pathogenesis and structural divergence from human kinases. This study integrates quantitative structure–activity relationship (QSAR) modeling and molecular docking to identify and prioritize potent TgCDPK1 inhibitors. A robust QSAR model was developed from a data set of 152 ligands, leveraging a systematic feature selection process to identify 23 key molecular descriptors predictive of inhibitory activity (R = 0.895, R² = 0.802). Molecular docking studies further characterized the binding interactions of top-ranked ligands, revealing strong binding affinities and favorable ADMET profiles. Notably, compound L03, identified as a substituted imidazopyrimidine derivative, demonstrated exceptional binding energy (−176.794 kcal/mol) and stability within the TgCDPK1 active site. Key interactions with Asp210(A) through hydrogen bonds and hydrophobic contacts were instrumental in its high binding affinity, underscoring its potential as a lead compound. These findings provide a comprehensive framework for rational drug design, combining computational approaches to accelerate the discovery of selective and efficacious anti-toxoplasma agents targeting TgCDPK1. This integrated methodology represents a significant advancement toward addressing the unmet clinical needs of toxoplasmosis treatment.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144716613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Antimicrobial Resistance in Klebsiella pneumoniae in the South African Populations: A Systematic Review and Meta-Analysis of Surveillance Studies 南非人群中肺炎克雷伯菌抗菌素耐药性的流行：监测研究的系统回顾和荟萃分析

IF 3.9 3区生物学

MicrobiologyOpen Pub Date : 2025-07-24 DOI: 10.1002/mbo3.70037

Sinethemba H. Yakobi, Uchechukwu U. Nwodo

{"title":"Prevalence of Antimicrobial Resistance in Klebsiella pneumoniae in the South African Populations: A Systematic Review and Meta-Analysis of Surveillance Studies","authors":"Sinethemba H. Yakobi, Uchechukwu U. Nwodo","doi":"10.1002/mbo3.70037","DOIUrl":"https://doi.org/10.1002/mbo3.70037","url":null,"abstract":"Antimicrobial resistance (AMR) poses a critical global health threat, with Klebsiella pneumoniae emerging as a high-priority pathogen due to escalating resistance rates. This systematic review and meta-analysis evaluated the AMR profiles of K. pneumoniae isolates from South Africa, a resource-limited setting where AMR burdens remain understudied. A comprehensive search of PubMed, Web of Science, and Google Scholar (January 2000 to June 2024) identified studies reporting resistance data. Nineteen studies comprising 9402 isolates were included, and data were analyzed using random-effects models. Pooled resistance prevalence was highest for amoxicillin (69.3%; 95% CI: 64.1%–74.1%), followed by second-generation cephalosporins (70.9%; 95% CI: 65.3%–75.8%), trimethoprim-sulfamethoxazole (57.8%; 95% CI: 52.4%–63.0%), and carbapenems, with imipenem resistance at 33.2% (95% CI: 28.5%–38.3%). Significant heterogeneity was observed (I² > 90%, p < 0.001), likely due to differences in study populations such as clinical versus environmental isolates, and regional prescribing practices. Publication bias was detected (Egger's test: p = 1.45 × 10⁻¹⁴), indicating possible underreporting of small-scale studies with null findings. These findings highlight alarming resistance rates to first-line antibiotics in South Africa and underscore the urgent need for multisectoral interventions. Priority actions should include standardized AMR surveillance to harmonize data collection, expanded antimicrobial stewardship programs particularly in high-resistance settings such as hospitals, and greater investment in novel therapies targeting carbapenem-resistant strains. Addressing methodological heterogeneity and minimizing publication bias in future research will be critical to strengthening the evidence base for informed policymaking.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70037","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analyzing the Challenges and Opportunities Associated With Harnessing New Antibiotics From the Fungal Microbiome 分析利用真菌微生物组新抗生素的挑战和机遇

IF 3.9 3区生物学

MicrobiologyOpen Pub Date : 2025-07-23 DOI: 10.1002/mbo3.70034

Md. Sakhawat Hossain, Md. Al Amin, Sirajul Islam, Hasan Imam, Liton Chandra Das, Shahin Mahmud

{"title":"Analyzing the Challenges and Opportunities Associated With Harnessing New Antibiotics From the Fungal Microbiome","authors":"Md. Sakhawat Hossain, Md. Al Amin, Sirajul Islam, Hasan Imam, Liton Chandra Das, Shahin Mahmud","doi":"10.1002/mbo3.70034","DOIUrl":"https://doi.org/10.1002/mbo3.70034","url":null,"abstract":"The rapid rise in antibiotic resistance is a critical global health issue, and few new classes of antibiotics have been discovered since 1990 compared to the antibiotic's golden era between 1950 and 1970. However, developing new antimicrobial compounds faces many challenges, improvements in cultivation methods, genetic engineering, and advanced technologies are opening new paths for discovering and producing effective antibiotics. This study focuses on the fungal microbiome as a promising source of new antibiotics. We explored historical developments and advanced genetic techniques to reveal the potential of fungi in antibiotic production. Although isolating and scaling up fungal antibiotic production presents challenges, innovative approaches like in situ separation during fermentation can effectively address these issues. Our research highlights the importance of understanding fungal communication and metabolite sharing to enhance antibiotic yields and the connection of cutting-edge technologies in accelerating the discovery and optimization of antibiotic-producing fungi. By focusing on these technical aspects and fostering teamwork across various fields, this study aims to overcome current obstacles, and advance the development of antibiotic production technologies.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70034","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144681181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas syringae Lipopolysaccharide Synthesis Gene wbpL Displays Heterogeneous Expression Within In Vitro and In Planta Populations 丁香假单胞菌脂多糖合成基因wbpL在离体和植物群体中呈现异质表达

IF 3.9 3区生物学

MicrobiologyOpen Pub Date : 2025-07-22 DOI: 10.1002/mbo3.70031

Laura Mancera-Miranda, José S. Rufián, Nieves López-Pagán, Javier Ruiz-Albert, Carmen R. Beuzón

{"title":"Pseudomonas syringae Lipopolysaccharide Synthesis Gene wbpL Displays Heterogeneous Expression Within In Vitro and In Planta Populations","authors":"Laura Mancera-Miranda, José S. Rufián, Nieves López-Pagán, Javier Ruiz-Albert, Carmen R. Beuzón","doi":"10.1002/mbo3.70031","DOIUrl":"https://doi.org/10.1002/mbo3.70031","url":null,"abstract":"Phenotypic heterogeneity usually refers to phenotypic variation not associated with genetic variation, nor induced by environmental stimuli. The phenotypic heterogeneity processes described for some complex bacterial traits are causing a shift in how bacterial phenotypes are studied, from traditional assessments by averaging populations to single-cell analysis focused on bacterial individual phenotypes and how these distribute within the population. The structure of the lipopolysaccharide (LPS) layer on the outer membrane in Gram-negative bacteria is often subjected to phenotypic variation, a process that is critical for virulence in animal pathogens. Here, we apply single-cell expression analyses to wbpL, a conserved Pseudomonas syringae glycosyltransferase-encoding gene essential for the synthesis of the O-antigen component of LPS. We show that expression of wbpL displays phenotypic heterogeneity in P. syringae pv. phaseolicola growing in rich medium and reaches bistable expression in minimal medium, where the population splits into WbpLON and WpbLOFF subpopulations. In planta, wbpL expression is also heterogeneous, displaying intermingled ON/OFF with comparable viability. Finally, we followed the expression of wbpL within the spatial context of apoplastic microcolonies, and not only detected heterogeneity within each microcolony, but also found that microcolonies displayed overall differences in fluorescence intensity that correlated with size, with smaller microcolonies displaying higher levels of wbpL expression.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70031","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating Effects of Antibiotics Across Preclinical Models of the Human Gastrointestinal Microbiota 评估抗生素对人类胃肠道微生物群临床前模型的影响

IF 3.9 3区生物学

MicrobiologyOpen Pub Date : 2025-07-16 DOI: 10.1002/mbo3.70030

Thomas A. Auchtung, Armando I. Lerma, Keegan Schuchart, Jennifer M. Auchtung

{"title":"Evaluating Effects of Antibiotics Across Preclinical Models of the Human Gastrointestinal Microbiota","authors":"Thomas A. Auchtung, Armando I. Lerma, Keegan Schuchart, Jennifer M. Auchtung","doi":"10.1002/mbo3.70030","DOIUrl":"https://doi.org/10.1002/mbo3.70030","url":null,"abstract":"While antibiotics play important roles in treating infections, disruption of the gastrointestinal microbiota during antibiotic treatment can lead to negative health consequences. However, for many antibiotics, the spectrum of activity has been determined for select isolates rather than for the range of microbes that populate the gastrointestinal tract. Here, we examined the response of communities of gastrointestinal microbes to antibiotics using two different model systems, human fecal minibioreactors and human microbiota-associated mice. Communities established in minibioreactors using 12 different fecal donors were exposed to 12 different classes of antibiotics. Samples from three fecal donors were used to colonize germ-free mice from three different genetic backgrounds; progeny mice were then exposed to 6 of 12 antibiotics tested in minibioreactors. Initial bacterial community diversity was dependent on both the fecal donor and model system. Antibiotics affected a wide range of taxa across the phylogenetic spectrum, with many taxa similarly affected across treatments with different classes of antibiotics. Vancomycin, typically administered to treat Gram-positive bacterial infections, decreased the abundance of diverse taxa, including Gram-negative Bacteroidota species. Effects on some taxa were restricted by model system, indicating the importance of environmental context on antibiotic susceptibility. Altogether, these results indicate the complex interrelationships between microbiota composition and environmental context on antibiotic susceptibility and demonstrate strengths and weaknesses of each preclinical model system for evaluating effects of new antibiotics and other compounds with potential to disrupt the microbiota.","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":3.9,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0