MicrobiologyOpen最新文献

{"title":"Prevalence, Diagnostic Accuracy, and Antimicrobial Resistance Patterns of Uropathogens in Urinary Tract Infections at a Health Facility in Kumasi, Ghana","authors":"Michael Nana Antwi-Boasiako,&nbsp;Frederick Ayensu,&nbsp;Aaron Awuah,&nbsp;Abdul-Hakim Mutala,&nbsp;Kwadwo Boampong,&nbsp;Kingsley Badu","doi":"10.1002/mbo3.70028","DOIUrl":"https://doi.org/10.1002/mbo3.70028","url":null,"abstract":"<p>Urinary tract infections (UTIs) are one of the most prevalent infections in both men and women. The most common causative organisms are <i>Escherichia coli, Klebsiella</i> spp., and <i>Staphylococcus</i> spp. UTIs are diagnosed using various diagnostic methods with urine culture as the gold standard. This study aimed to estimate the prevalence of UTIs, compare the tools employed in the diagnosis of UTIs, and assess the antibiotic susceptibility pattern of bacterial isolates among patients in a local health facility in Kumasi, Ghana. A cohort of 208 participants were enlisted for a cross-sectional study at the HopeXchange Medical Centre, Kumasi. Mid-stream urine samples were collected from participants and analyzed by culture, microscopy, and dipstick methods. Culture isolates were tested on Mueller-Hinton Agar applying the Kirby Bauer method against a range of antibiotics. The prevalence of <i>Staphylococcus</i> spp. (25%) and <i>Klebsiella</i> spp. (16.7%) were higher than that of <i>Escherichia coli</i> (8.3%). UTI was more common in individuals between 50 and 59 years of age (36.5%) and mostly associated among women (76.9%). Overall, culture confirmed UTI prevalence was 23.08% while dispstick and microscopy diagnosed 44.23% and 47.60% of UTIs, respectively. Microscopy exhibited a sensitivity of 68.8% and specificity of 58.8% while the dipstick had a sensitivity of 60.4% and specificity of 60.6% compared to urine culture. Meropenem showed 100% susceptibility, while high resistance was observed for chloramphenicol and ceftriaxone against <i>Escherichia coli, Staphylococcus</i> spp<i>., Providencia</i> spp., and <i>Klebsiella</i> spp. UTI was significantly associated with age, gender, and the standard diagnostic method (<i>p</i> &lt; 0.05).</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 5","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Cover Image","authors":"Fatih Mehmet Akıllı,&nbsp;Arzu İlki","doi":"10.1002/mbo3.70055","DOIUrl":"https://doi.org/10.1002/mbo3.70055","url":null,"abstract":"<p><b>Front cover caption</b>: The cover image is based on the article <i>Evaluation of Colistin Susceptibility in Carbapenem-Resistant Acinetobacter baumannii Isolates Using Broth Microdilution, MICRONAUT-MIC-Strip, and VITEK MS</i> by Fatih Mehmet AKILLI et al., 10.1002/mbo3.70046.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back Cover Image","authors":"Hye Rim Yeo,&nbsp;Mee Young Shin,&nbsp;Juri Kim,&nbsp;Soon-Jung Park","doi":"10.1002/mbo3.70054","DOIUrl":"https://doi.org/10.1002/mbo3.70054","url":null,"abstract":"<p><b>Inside front cover caption</b>: The cover image is based on the article <i>Two Centrins and Their Posttranslational Modification Modulate the Cell Cycle of Giardia lamblia</i> by Juri Kim et al., 10.1002/mbo3.70038.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure>\u0000 </p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144897637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Hypervirulent Klebsiella pneumoniae Isolates Using Multilocus Sequence Typing in West of Iran","authors":"Mohammad Hossein Haddadi,&nbsp;Nourkhoda Sadeghifard,&nbsp;Saeed Khoshnood,&nbsp;Abbas Maleki,&nbsp;Sobhan Ghafourian,&nbsp;Hassan Valadbeigi","doi":"10.1002/mbo3.70049","DOIUrl":"https://doi.org/10.1002/mbo3.70049","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Hypervirulent <i>Klebsiella pneumonia</i> (hvKP) has been considered due to severe infections such as pyogenic liver abscess, pneumonia, meningitis, endophthalmitis and necrotizing fasciitis. Due to the importance of the subject, hv<i>KP</i> isolates were investigated in the current study.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A total of 52 non-repetitive <i>Klebsiella pneumonia (K. pneumonia</i>) isolates were collected from clinical specimens (pleural fluid, blood, urine and trachal aspiration) of hospitalized patients in three well-known hospitals in Ilam, west of Iran. Phenotypic tests, including the string test and potassium tellurite resistance, and molecular methods such as polymerase chain reaction (PCR) for <i>iucA</i>, <i>iroB</i>, <i>iutA</i>, and <i>rmpA</i> genes were used for hvKP diagnosis. In addition, multilocus sequence typing (MLST) was used to clarify the genetic relationships between isolates.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The <i>mrkD</i>, <i>iroB</i>, <i>iucA</i>, <i>iutA</i>, and <i>rmpA</i> genes were present in 6 (11.53%), 4 (7.7%), 12 (23.1%), 5 (9.6%), and 8 (15.4%) of the <i>K. pneumoniae</i> isolates, respectively. Eight isolates from different sources were identified with new sequence types (STs). These newly identified STs include ST-7466, ST-7467, ST-7468, ST-7469, ST-7470, ST-7471, ST-7472 and ST-7473.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>We reported the eight new STs based on the MLST method for the phylogenetics of hvKP isolates. Our data indicated that no correlation between the phylogenetics of hvKP isolates. So, more extensive studies are needed to better understand the epidemiology of hvKP.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144869711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Diversity Scaling in Lung Cancer Microbiome Across Individuals and Tissue Types","authors":"Jiandong Mei,&nbsp;Yuting Qiao,&nbsp;Zhanshan (Sam) Ma","doi":"10.1002/mbo3.70036","DOIUrl":"https://doi.org/10.1002/mbo3.70036","url":null,"abstract":"<p>The intra-tumor microbiome can impact the tumor's behavior by influencing its growth, inflammatory reactions, evasion of the immune system, genomic instability, and drug resistance. Altering this microbiota to improve the response to cancer treatment could offer fresh perspectives on cancer therapy. The very first step in intervening in the microbiome is to gain a deep understanding of how microbial diversity varies spatially and temporally between tissues or among individuals. Such changes can be investigated with the so-termed diversity–area relationship (DAR) modeling (Ma. 2018. <i>Ecology and Evolution</i>, 8(20), 10023–10038). This included examining the DAR profiles, Pairwise Diversity Overlap (PDO) profiles, Maximum Accumulated Diversity (MAD) profiles, and the ratio of local to global accumulated diversity (LGD) profiles. This study applies the DAR approach to reanalyze five lung tissue microbiome datasets to shed light on how the microbial diversity changes across tissue types and across individual patients. We characterized the diversity scaling of human lung cancer microbiota from aspects such as microbial community diversity, variation rates of diversity, similarity, and microbial community proportions. The generated results indicate that there are no statistically significant differences in the DAR scaling parameters across different tissue types, suggesting that the diversity scaling of microbial communities in normal and tumor tissues across individuals seems to be invariant. The invariance is simply a reflection of the resilience of lung tissue microbiome against disturbance such as lung cancer, and thus further studies at the level of microbial species to better understand their relationship with cancer are critical.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70036","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Azotobacter vinelandii as a Nitrogen-Negative Chassis for Bio-Oil and Bio-Wax Production of Heterologous and Native Lipids","authors":"Brett M. Barney,&nbsp;Bilge Bahar Camur,&nbsp;Lucas J. Stolp,&nbsp;Natalia Calixto Mancipe,&nbsp;Benjamin R. Dietz","doi":"10.1002/mbo3.70047","DOIUrl":"https://doi.org/10.1002/mbo3.70047","url":null,"abstract":"<p>The biosynthetic production of energy-dense petrochemical substitutes is an important goal to address sustainability. The diazotrophic soil microbe <i>Azotobacter vinelandii</i> is a model microbe for the study of biological nitrogen fixation. In addition to capturing atmospheric nitrogen and converting it into usable nitrogen compounds, it is also regarded for the ability to accumulate the bioplastic poly-β-hydroxybutyrate and the extracellular polysaccharide alginate. Here, we demonstrate the potential to broaden the chemical products repertoire of <i>A. vinelandii</i> by demonstrating the accumulation of several classes of biological lipids and waxes. These products include the expanded accumulation of wax esters and fatty alcohols through heterologous expression of foreign genes and pathways, and increased production of the native lipid alkylresorcinol, accomplished by deregulating specific internal pathways and removing competitive pathways for alternative products. As a result, we demonstrate a sevenfold increase in the accumulation of alkylresorcinol, manifesting as intracellular inclusions that are easily extracted with simple solvents and account for nearly 20% of the cellular biomass. By selecting a diazotrophic microbe as a chassis for lipid accumulation, we produced these lipids without any requirement for industrial nitrogen sources in the growth medium, resulting in a net positive nitrogen process as well.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70047","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Characterization and Genome Analysis of Pseudomonas loganensis sp. nov., a Novel Endophytic Bacterium","authors":"Melisa Z. Karaman,&nbsp;Ahmet E. Yetiman,&nbsp;Jixun Zhan,&nbsp;Ozkan Fidan","doi":"10.1002/mbo3.70051","DOIUrl":"https://doi.org/10.1002/mbo3.70051","url":null,"abstract":"<p><i>Pseudomonas</i> species are highly adaptable, thriving in diverse environments and exhibiting remarkable genetic and metabolic diversity. While some strains are pathogenic, others have significant ecological and industrial applications. Bioinformatics and biochemical analyses, including antibiotic sensitivity testing, revealed that <i>Pseudomonas loganensis</i> sp. nov. can tolerate NaCl concentrations up to 5% and pH ranges between 5 and 9. Antibiogram results corroborated genome data, demonstrating resistance to vancomycin, ampicillin, methicillin, oxacillin, and penicillin G. Phylogenetic analysis based on 16S rRNA, <i>rpoB</i>, <i>rpoD</i>, and <i>gyrB</i> genes, combined with average nucleotide identity (ANI) comparisons, confirmed <i>P. loganensis</i> sp. nov. as a novel species within the <i>Pseudomonas</i> genus. Genome analysis further revealed the presence of turnerbactin and carotenoid gene clusters. Turnerbactin, known to contribute to nitrogen fixation in plants, highlights the strain's potential as a biofertilizer. Additionally, the carotenoid gene cluster suggests potential applications in industrial carotenoid production. The discovery of a trehalose synthase (<i>treS</i>) gene indicates the capability for one-step conversion of maltose into trehalose, underscoring its potential utility in trehalose production.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Infection by Porphyromonas gingivalis and Fusobacterium nucleatum Increases PD-L1 Expression in Esophageal Cancer Tissues","authors":"Xiayi Lin,&nbsp;Xiaohui Chen,&nbsp;Mengyuan Chen,&nbsp;Yiqiu Li,&nbsp;Yu Deng,&nbsp;Bohong Xian,&nbsp;Zijun Li","doi":"10.1002/mbo3.70050","DOIUrl":"https://doi.org/10.1002/mbo3.70050","url":null,"abstract":"<p>Currently, the impact of the microbiome on the progression and recurrence of esophageal cancer remains unclear. Microbes have been demonstrated to increase programmed death-ligand 1 (PD-L1) expression in certain epithelial tumors, thereby facilitating immune escape. Therefore, we hypothesized that the microbiome in esophageal tissues might be involved in the progression and recurrence of esophageal squamous cell carcinoma (ESCC) through PD-L1 upregulation. To assess this possibility, we analyzed the relationship between the microbial community composition and PD-L1 expression. We found that the microbiome of esophageal cancer tissue significantly differs from that of normal tissue, and <i>Porphyromonas gingivalis</i> and <i>Fusobacterium nucleatum</i> could represent markers of ESCC. An immunohistochemical assay revealed that <i>P. gingivalis</i> and <i>F. nucleatum</i> infection led to a significant increase in PD-L1 expression on the membrane surface of tumor cells, and this finding was further confirmed in cell experiments. Our findings suggest that eliminating intratumoral <i>P. gingivalis</i> and <i>F. nucleatum</i> infection could represent an important therapeutic strategy for reducing the progression and recurrence of esophageal cancer.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Correlation Between Carbohydrate Loading Diet and Gut Microbiome: A Systematic Review","authors":"Omar El-Kholy,&nbsp;Lindsey Nichols,&nbsp;Ahmed Adham R. Elsayed,&nbsp;Marc D. Basson","doi":"10.1002/mbo3.70045","DOIUrl":"https://doi.org/10.1002/mbo3.70045","url":null,"abstract":"<p>The gut microbiome critically influences digestion, mucosal permeability, metabolism, blood pressure, and lipid profile. Pathological shifts in these processes cause metabolic syndrome, a growing human health concern that may be modeled in animals with carbohydrate-loading diets. We reviewed the effects of carbohydrate-loading diets on the animal gut microbiome. A systematic literature search was performed up to September 2024 on five databases: Cochrane Library, PubMed, Scopus, Web of Science, and VHL. Relevant animal studies were assessed for risk of bias using SYRCLE's tool. Seventeen studies were included, with data from more than 690 rodents. Carbohydrate-loading diets alter the gut microbiome composition, diversity, and ratios. High-carbohydrate, high-fat diets were almost consistently associated with an increased Firmicutes to Bacteroidetes (F/B) ratio. Different types of carbohydrates, such as fructose, sucrose, or even special diet types, vary widely in impacting both the microbiota and microbiota-associated pathophysiology, inducing different metabolic states and affecting blood pressure, gut structural integrity, immunomodulation, and other functions. Interventions added with or after feeding substantially modulated these diet-induced changes. Carbohydrate-loading diets can differentially influence the gut microbiome and associated physiology. High-fat carbohydrate diets, apart from starch-based diets, typically increase the F/B ratio, a shift linked to human obesity. In contrast, low-fat carbohydrate diets do not elevate the F/B ratio but instead produce diverse microbiome effects, ranging from beneficial to harmful, depending on the carbohydrate type and other influencing factors. Further animal and human research is crucial to validate and further illuminate the dietary impact on the gut microbiome.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144782669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological Preparedness for Novel Pandemics: Diagnostic Performance of IVDS Against SARS-CoV-2","authors":"Murat Kavruk,&nbsp;Meltem Ercan,&nbsp;Barış Ata Borsa,&nbsp;Veli Cengiz Özalp,&nbsp;Frank J. Hernandez","doi":"10.1002/mbo3.70042","DOIUrl":"https://doi.org/10.1002/mbo3.70042","url":null,"abstract":"<p>Although the COVID-19 pandemic has created many challenges and negative impacts around the world, some of which will persist into the future, its technological challenge has created a unique opportunity in a globalized world. It is a rare event that almost all of humanity to be directed towards a single goal and to try to produce solutions, but the necessity of a similar global action in the future has begun to enter the agenda again. The predictions made on the basis of countries and institutions against the possibility of a pandemic, which is defined as Disease X, are shaped by the experience of the COVID-19 pandemic. Technologically, one of the know-how we have gained in this pandemic is the performance of IVD and test systems in terms of quality and quantity. A comprehensive analysis of the products produced by combining biotechnology with different strategies has not been conducted. In this context, we have analyzed the technical preferences, limitations, and other performance parameters of IVDs and test kits that could be developed against a future Disease X. The performance parameters of 2,882 biotechnological products listed for use in the European Union have been analyzed, and areas that could be targeted for increased effectiveness have been identified. Our study is the first of its kind in this field and can serve as a guide for those who want to work on detection methods, diagnostics, and novel technologies for deployment in future pandemics.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70042","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}