{"title":"Co-Infection by Porphyromonas gingivalis and Fusobacterium nucleatum Increases PD-L1 Expression in Esophageal Cancer Tissues","authors":"Xiayi Lin, Xiaohui Chen, Mengyuan Chen, Yiqiu Li, Yu Deng, Bohong Xian, Zijun Li","doi":"10.1002/mbo3.70050","DOIUrl":null,"url":null,"abstract":"<p>Currently, the impact of the microbiome on the progression and recurrence of esophageal cancer remains unclear. Microbes have been demonstrated to increase programmed death-ligand 1 (PD-L1) expression in certain epithelial tumors, thereby facilitating immune escape. Therefore, we hypothesized that the microbiome in esophageal tissues might be involved in the progression and recurrence of esophageal squamous cell carcinoma (ESCC) through PD-L1 upregulation. To assess this possibility, we analyzed the relationship between the microbial community composition and PD-L1 expression. We found that the microbiome of esophageal cancer tissue significantly differs from that of normal tissue, and <i>Porphyromonas gingivalis</i> and <i>Fusobacterium nucleatum</i> could represent markers of ESCC. An immunohistochemical assay revealed that <i>P. gingivalis</i> and <i>F. nucleatum</i> infection led to a significant increase in PD-L1 expression on the membrane surface of tumor cells, and this finding was further confirmed in cell experiments. Our findings suggest that eliminating intratumoral <i>P. gingivalis</i> and <i>F. nucleatum</i> infection could represent an important therapeutic strategy for reducing the progression and recurrence of esophageal cancer.</p>","PeriodicalId":18573,"journal":{"name":"MicrobiologyOpen","volume":"14 4","pages":""},"PeriodicalIF":4.6000,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/mbo3.70050","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"MicrobiologyOpen","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mbo3.70050","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Currently, the impact of the microbiome on the progression and recurrence of esophageal cancer remains unclear. Microbes have been demonstrated to increase programmed death-ligand 1 (PD-L1) expression in certain epithelial tumors, thereby facilitating immune escape. Therefore, we hypothesized that the microbiome in esophageal tissues might be involved in the progression and recurrence of esophageal squamous cell carcinoma (ESCC) through PD-L1 upregulation. To assess this possibility, we analyzed the relationship between the microbial community composition and PD-L1 expression. We found that the microbiome of esophageal cancer tissue significantly differs from that of normal tissue, and Porphyromonas gingivalis and Fusobacterium nucleatum could represent markers of ESCC. An immunohistochemical assay revealed that P. gingivalis and F. nucleatum infection led to a significant increase in PD-L1 expression on the membrane surface of tumor cells, and this finding was further confirmed in cell experiments. Our findings suggest that eliminating intratumoral P. gingivalis and F. nucleatum infection could represent an important therapeutic strategy for reducing the progression and recurrence of esophageal cancer.
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MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era.
The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes.
MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to:
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MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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