Prevalence, Diagnostic Accuracy, and Antimicrobial Resistance Patterns of Uropathogens in Urinary Tract Infections at a Health Facility in Kumasi, Ghana

IF 4.6 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2025-08-27 DOI:10.1002/mbo3.70028

Michael Nana Antwi-Boasiako, Frederick Ayensu, Aaron Awuah, Abdul-Hakim Mutala, Kwadwo Boampong, Kingsley Badu

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Abstract

Urinary tract infections (UTIs) are one of the most prevalent infections in both men and women. The most common causative organisms are Escherichia coli, Klebsiella spp., and Staphylococcus spp. UTIs are diagnosed using various diagnostic methods with urine culture as the gold standard. This study aimed to estimate the prevalence of UTIs, compare the tools employed in the diagnosis of UTIs, and assess the antibiotic susceptibility pattern of bacterial isolates among patients in a local health facility in Kumasi, Ghana. A cohort of 208 participants were enlisted for a cross-sectional study at the HopeXchange Medical Centre, Kumasi. Mid-stream urine samples were collected from participants and analyzed by culture, microscopy, and dipstick methods. Culture isolates were tested on Mueller-Hinton Agar applying the Kirby Bauer method against a range of antibiotics. The prevalence of Staphylococcus spp. (25%) and Klebsiella spp. (16.7%) were higher than that of Escherichia coli (8.3%). UTI was more common in individuals between 50 and 59 years of age (36.5%) and mostly associated among women (76.9%). Overall, culture confirmed UTI prevalence was 23.08% while dispstick and microscopy diagnosed 44.23% and 47.60% of UTIs, respectively. Microscopy exhibited a sensitivity of 68.8% and specificity of 58.8% while the dipstick had a sensitivity of 60.4% and specificity of 60.6% compared to urine culture. Meropenem showed 100% susceptibility, while high resistance was observed for chloramphenicol and ceftriaxone against Escherichia coli, Staphylococcus spp., Providencia spp., and Klebsiella spp. UTI was significantly associated with age, gender, and the standard diagnostic method (p < 0.05).

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加纳库马西一家卫生机构尿路感染中尿路病原体的患病率、诊断准确性和抗微生物药物耐药性模式

尿路感染（uti）是男性和女性中最普遍的感染之一。最常见的病原菌是大肠杆菌、克雷伯氏菌和葡萄球菌。尿路感染的诊断方法多种多样，以尿培养为金标准。本研究旨在估计尿路感染的患病率，比较用于诊断尿路感染的工具，并评估加纳库马西当地卫生机构患者中细菌分离株的抗生素敏感性模式。在库马西的HopeXchange医疗中心招募了208名参与者进行横断面研究。收集参与者的中游尿液样本，并通过培养、显微镜和试纸法进行分析。用Kirby Bauer法在Mueller-Hinton琼脂上测试培养分离物对一系列抗生素的抗性。葡萄球菌（25%）和克雷伯氏菌（16.7%）的感染率高于大肠杆菌（8.3%）。尿路感染在50 - 59岁的人群中更为常见（36.5%），其中女性居多（76.9%）。总体而言，培养确诊的UTI患病率为23.08%，而贴片和显微镜诊断的UTI患病率分别为44.23%和47.60%。与尿培养相比，镜检的敏感性为68.8%，特异性为58.8%，而试纸的敏感性为60.4%，特异性为60.6%。美罗培南的敏感性为100%，而氯霉素和头孢曲松对大肠埃希菌、葡萄球菌、普罗维登氏菌和克雷伯氏菌的耐药率较高。UTI与年龄、性别、标准诊断方法相关（p < 0.05）。

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

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