用多位点序列分型评价伊朗西部地区高致病性肺炎克雷伯菌分离株

IF 4.6 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2025-08-19 DOI:10.1002/mbo3.70049

Mohammad Hossein Haddadi, Nourkhoda Sadeghifard, Saeed Khoshnood, Abbas Maleki, Sobhan Ghafourian, Hassan Valadbeigi

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引用次数: 0

摘要

高致病性肺炎克雷伯氏菌（hvKP）被认为是由于严重感染，如化脓性肝脓肿、肺炎、脑膜炎、眼内炎和坏死性筋膜炎。由于该课题的重要性，本研究对hvKP分离株进行了调查。方法从伊朗西部伊拉姆市3家知名医院住院患者的临床标本（胸膜液、血液、尿液和气管抽吸）中分离出52株非重复性肺炎克雷伯菌（肺炎克雷伯菌）。hvKP的诊断采用表型试验，包括串试验和抗碲酸钾试验，以及iucA、iroB、iutA和rmpA基因的聚合酶链反应（PCR）等分子方法。此外，还利用多位点序列分型（MLST）明确了分离株间的遗传关系。结果mrkD、iroB、iucA、iutA和rmpA基因分别在6株（11.53%）、4株（7.7%）、12株（23.1%）、5株（9.6%）和8株（15.4%）中存在。从不同来源分离出8株具有新的序列类型（STs）。这些新鉴定的st包括ST-7466， ST-7467, ST-7468, ST-7469, ST-7470, ST-7471， ST-7472和ST-7473。结论利用MLST方法对hvKP分离株进行系统发育分析，获得8个新的STs。我们的数据表明hvKP分离株的系统发育没有相关性。因此，需要更广泛的研究来更好地了解hvKP的流行病学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Assessment of Hypervirulent Klebsiella pneumoniae Isolates Using Multilocus Sequence Typing in West of Iran

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Assessment of Hypervirulent Klebsiella pneumoniae Isolates Using Multilocus Sequence Typing in West of Iran

Background

Hypervirulent Klebsiella pneumonia (hvKP) has been considered due to severe infections such as pyogenic liver abscess, pneumonia, meningitis, endophthalmitis and necrotizing fasciitis. Due to the importance of the subject, hvKP isolates were investigated in the current study.

Methods

A total of 52 non-repetitive Klebsiella pneumonia (K. pneumonia) isolates were collected from clinical specimens (pleural fluid, blood, urine and trachal aspiration) of hospitalized patients in three well-known hospitals in Ilam, west of Iran. Phenotypic tests, including the string test and potassium tellurite resistance, and molecular methods such as polymerase chain reaction (PCR) for iucA, iroB, iutA, and rmpA genes were used for hvKP diagnosis. In addition, multilocus sequence typing (MLST) was used to clarify the genetic relationships between isolates.

Results

The mrkD, iroB, iucA, iutA, and rmpA genes were present in 6 (11.53%), 4 (7.7%), 12 (23.1%), 5 (9.6%), and 8 (15.4%) of the K. pneumoniae isolates, respectively. Eight isolates from different sources were identified with new sequence types (STs). These newly identified STs include ST-7466, ST-7467, ST-7468, ST-7469, ST-7470, ST-7471, ST-7472 and ST-7473.

Conclusions

We reported the eight new STs based on the MLST method for the phylogenetics of hvKP isolates. Our data indicated that no correlation between the phylogenetics of hvKP isolates. So, more extensive studies are needed to better understand the epidemiology of hvKP.

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

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