Microbiological research最新文献

{"title":"Biological characterization and stability of three lytic wastewater-derived bacteriophages targeting multidrug-resistant Acinetobacter baumannii and A. johnsonii clinical strains","authors":"Martyna Cieślik ,&nbsp;Olaf Bajrak ,&nbsp;Michał Wójcicki ,&nbsp;Filip Orwat ,&nbsp;Norbert Łodej ,&nbsp;Stanisław Błażejak ,&nbsp;Edyta Pawlak ,&nbsp;Krzysztof J. Pawlik ,&nbsp;Andrzej Górski ,&nbsp;Ewa Jończyk-Matysiak","doi":"10.1016/j.micres.2025.128320","DOIUrl":"10.1016/j.micres.2025.128320","url":null,"abstract":"<div><div>Due to the rapidly increasing problem of pathogen resistance to drugs, including bacterial resistance to antibiotics, there is a growing need to explore new therapeutic solutions. One of the multidrug-resistant pathogens responsible for many difficult-to-treat infections is <em>Acinetobacter baumannii</em>. The clinical relevance of another species, <em>A. johnsonii</em>, is also increasingly being recognized. As the development pipeline for new antibiotics remains limited, alternative antimicrobial approaches are urgently required. Among these, the therapeutic use of bacteriophages against multidrug-resistant pathogens is currently gaining renewed interest. In this study, we describe the comprehensive characterization of three novel <em>Acinetobacter</em>-specific bacteriophages (Acba_19, Acjo_20 and Acba_21) isolated from sewage samples. The research were conducted using clinical strains obtained from various infection sites of patients in healthcare facilities in Poland. Genomic analysis revealed no markers of temperate phages in any of the isolates, allowing their classification as strictly lytic viruses. In addition to determining basic parameters of phages – such as lytic range, species specificity, virion morphology, adsorption dynamics and infection kinetics – we also conducted stability studies of phage preparations under various conditions. These studies included assessments of pH and temperature stability, evaluation of different cryoprotectants (such as trehalose, glycerol, and pluronic), as well as the effects of storage containers made of glass and plastic materials.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128320"},"PeriodicalIF":6.9,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeast proteomics: Advances and applications in alcoholic fermentation","authors":"Francisco José Martín-García ,&nbsp;María José Labrador-Valls ,&nbsp;Rosa María Barbero-Fernández ,&nbsp;Minami Ogawa ,&nbsp;Jaime Moreno-García","doi":"10.1016/j.micres.2025.128318","DOIUrl":"10.1016/j.micres.2025.128318","url":null,"abstract":"<div><div>Yeast proteomics has become a crucial approach for elucidating molecular mechanisms underlying alcoholic fermentation, widely applied in winemaking, brewing, and bioethanol production. Advances in high-throughput techniques, such as two-dimensional electrophoresis and mass spectrometry, have enabled detailed characterization of protein expression dynamics in fermenting yeast strains. These studies have improved strain classification, optimized fermentation conditions, and identified protein biomarkers linked to flavor and aroma profiles. Key findings include the upregulation of enzymes involved in glycolysis, ethanol production, amino acid, and sulfur metabolism—crucial for yeast adaptation to environmental stress. Notable stress-related proteins, such as heat shock proteins (Hsp12p, Hsp26p, Hsp60p), superoxide dismutases (Sod1p, Sod2p), redox regulators (Tsa1p, Ahp1p, Trx1p, Gnd1p), and proteins related to membrane and cell wall integrity (Cwp1p, Erg11p, Erg6p), have been shown to play pivotal roles. Central carbon metabolism enzymes (e.g., Pyk1p, Adh1p, Tdh family) are also prominently expressed. This growing body of knowledge highlights the potential of proteomic engineering to improve yeast performance and sensory outcomes. However, challenges remain in scaling laboratory findings to industrial processes and in implementing real-time proteomic monitoring. The integration of multi-omics and evolving analytical tools promises to advance fermentation science and its biotechnological applications.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128318"},"PeriodicalIF":6.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Putative SET domain-containing proteins play significant roles in regulating sporulation and pathogenicity in Phytophthora capsici","authors":"Bingting Lai ,&nbsp;Hengyuan Guo ,&nbsp;Kaidi Zhang ,&nbsp;Huirong Wang ,&nbsp;Xiang Qiu ,&nbsp;Lili Lin ,&nbsp;Ruqi Shi ,&nbsp;Moosa Sedibe ,&nbsp;Youxing Zou ,&nbsp;Justice Norvienyeku","doi":"10.1016/j.micres.2025.128316","DOIUrl":"10.1016/j.micres.2025.128316","url":null,"abstract":"<div><div><em>Phytophthora capsici</em> is a filamentous oomycete responsible for root rot, fruit rot, leaf blight, and other economically destructive diseases in multiple plant species, including pepper (<em>Capsicum annuum),</em> tomato (<em>Solanum lycopersicum</em>), squash (<em>Cucurbita pepo</em>), eggplant (<em>Solanum melongena</em>), faba bean (<em>Vicia faba</em>), and lima bean (<em>Phaseolus lunatus</em>), among others. The pathogen causes significant yield losses in fruit and vegetable crops globally. Multiple molecular parameters, including effector proteins and epigenetic modulators, play vital roles in modulating the physio pathological development of <em>P. capsici</em>. Here, we identified 56 SET domain-containing proteins in <em>P. capsici</em>, with 35 predicted as potential effectors. Transcriptomic analysis revealed the upregulation of 13 candidate effector genes during infection, suggesting their roles in pathogenicity. We successfully deleted one effector, <em>PcSET-C</em> (DVH05_022087), and two non-effector proteins, <em>PcSET-B</em> (DVH05_004260), and <em>PcSET-A</em> (DVH05_000194), using CRISPR-Cas9 and homologous recombination strategies. Phenotypic analysis showed that targeted deletion of the <em>PcSET</em>-<em>A,</em> and <em>PcSET</em>-B gene significantly impaired vegetative growth, while Δ<em>Pcset-C</em> strains displayed growth like wild-type strains. Additionally, we showed that targeted disruption of all three genes resulted in reduced asexual sporulation and almost completely abolished the pathogenicity or virulence of Δ<em>Pcset-A</em>, Δ<em>Pcset-B</em>, and Δ<em>Pcset-C</em> strains on different <em>P. capsici-</em>susceptible plants<em>.</em> However, targeted replacement of the three SET domain-containing genes investigated in this study has no significant adverse effects on zoospore release in <em>P. capsici</em>. These findings provide insights into the crucial role of SET domain-containing proteins in both morphological and pathological development of <em>P. capsici</em> and underscore PcSETs as potential targets for disease control.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128316"},"PeriodicalIF":6.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144886597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akkermansia muciniphila: A key player in gut microbiota-based disease modulation","authors":"Nida Shaheen ,&nbsp;Waleed Khursheed ,&nbsp;Bijay Gurung ,&nbsp;Shaohua Wang","doi":"10.1016/j.micres.2025.128317","DOIUrl":"10.1016/j.micres.2025.128317","url":null,"abstract":"<div><div><em>Akkermansia muciniphila</em> (<em>A. muciniphila</em>), a mucin-degrading bacterium residing in the gut’s mucus layer, has emerged as a key modulator of host physiology with significant implications for health and disease. Growing evidence shows that <em>A. muciniphila</em> influences host metabolism, strengthens gut barrier integrity, modulates microbial composition, and regulates immune responses. This review synthesizes current literature on <em>A. muciniphila</em>, emphasizing its role in conditions such as metabolic disorders, inflammatory bowel disease (IBD), <em>Clostridioides difficile</em> infection (CDI), cancer, cardiovascular disease, and aging. In metabolic disorders, <em>A. muciniphila</em> improves insulin sensitivity, reduces adiposity, and increases GLP-1 secretion through mechanisms involving short-chain fatty acid (SCFA) production and TLR2 activation. It also restores microbial balance and reduces inflammation in type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). In IBD, it enhances mucus secretion, tight junction integrity, regulatory T cell expansion, and suppresses pro-inflammatory cytokines. In CDI, it promotes epithelial protection and colonization resistance by enriching butyrate producers. In cancer, it boosts immune checkpoint inhibitor efficacy by enhancing IL-12 and T cell activation. It also reduces vascular inflammation and calcification in cardiovascular disease via propionate production. In aging, <em>A. muciniphila</em> improves metabolic health, reduces chronic inflammation, promotes SCFA production, and preserves blood–brain barrier integrity. Both live and pasteurized forms are effective, with pasteurized, particularly Amuc_1100, showing enhanced benefits. Broader application requires large-scale trials, better understanding of host and strain variability, and development of personalized, synergistic therapies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128317"},"PeriodicalIF":6.9,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144878529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root specialized metabolites shape plant-beneficial bacterial communities to mitigate hydrocarbon stress","authors":"Xian Xiao ,&nbsp;Xiaowen Fang ,&nbsp;Ning Hu ,&nbsp;Ke Huang ,&nbsp;Ruilin Huang ,&nbsp;Muhammad Raza Farooq ,&nbsp;Hanbo Zhang ,&nbsp;Yuan Zhao","doi":"10.1016/j.micres.2025.128314","DOIUrl":"10.1016/j.micres.2025.128314","url":null,"abstract":"<div><div>Plant roots secrete various compounds to attract beneficial microbes from soil, enhancing resilience to environmental stresses. The mechanisms by which perennial grasses accumulate specific plant-beneficial bacteria under organic pollution remain unclear. We conducted a pot experiment using ryegrass grown in diesel-contaminated soils (0–15 g/kg) to analyze rhizosphere bacterial community and root exudate, using 16S rRNA gene amplicon sequencing and untargeted metabolomics. A significant increase in the relative abundance of rhizosphere plant-beneficial bacteria was observed along the contamination gradient (<em>r</em>² = 0.64, <em>p</em> &lt; 0.01), with bacterial taxa possessing dual capabilities in hydrocarbon degradation and nitrogen fixation, such as <em>Nocardioides</em>, becoming more dominant. We identified 22 core metabolites (defined as consistently differential metabolites that showed significant increase across all contamination treatments) including organoheterocyclic compounds, benzenoids, and phenylpropanoids. These core metabolites significantly predicted the dissimilarity of plant-beneficial bacterial communities (Mantel test, <em>r</em>² = 0.17, <em>p</em> &lt; 0.001). Additionally, we verified the chemotactic response of two plant-beneficial bacterial strains, <em>Rhodopseudomonas</em> sp. and <em>Pseudomonas</em> sp., towards eugenol (a benzenoid), 7-acetoxy-4-methylcoumarin (a phenylpropanoid), and benzil (a benzenoid). Among these, eugenol is a promising compound that selectively induces beneficial microbes, helping ryegrass cope with hydrocarbon stress. These findings advance beyond prior observations of general exudate changes by identifying conserved metabolic responses across contamination levels, and demonstrating their role in microbial recruitment via chemoattraction. Our study provided valuable insights into the potential application of rhizosphere engineering for phytoremediation of hydrocarbon-contaminated soils, as well as the development of microbial-based strategies to enhance crop resilience under environmental stresses.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128314"},"PeriodicalIF":6.9,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Auxin signaling activated by endophytic Cross-Kingdom synthetic microbiota improves the quality of Atractylodes chinensis","authors":"Meng Tang,&nbsp;Xu-Ying Yao,&nbsp;Jia-Yan Xu,&nbsp;Jia-Yun Wu,&nbsp;Kai Sun,&nbsp;Chuan-Chao Dai,&nbsp;Fei Chen","doi":"10.1016/j.micres.2025.128312","DOIUrl":"10.1016/j.micres.2025.128312","url":null,"abstract":"<div><div>Endophytes play a vital role in improving the quality of medicinal plants. Both endophytic fungi and bacteria can confer fitness benefits to medicinal plants; however, the effects of endophytic cross-kingdom synthetic microbiota on host development remain largely unexplored. In the present study, synthetic communities (SynComs) composed of endophytic fungi and/or bacteria were constructed from the medicinal plant <em>Atractylodes chinensis</em> using microbial culture techniques and pot experiments. The results showed that cross-kingdom SynComs comprising endophytic fungi and bacteria were more effective than single-kingdom fungal or bacterial SynComs in promoting the growth and accumulation of secondary metabolites in <em>A. chinensis</em>. Transcriptomic analysis further revealed that the photosynthesis and sesquiterpenoid biosynthesis pathways were upregulated by cross-kingdom SynComs. Notably, the genes related to auxin signal transduction were markedly activated. Chemical treatments, indole-3-acetic acid flux assays, and experiments using <em>Arabidopsis thaliana</em> mutants collectively showed that auxin signaling plays a pivotal role in mediating the enhanced growth and metabolism induced by cross-kingdom SynComs. Field experiments validated the effectiveness of cross-kingdom SynComs for improving the yield and quality of <em>A. chinensis</em>. This study highlights the critical role of endophytic fungi–bacteria–plant associations in promoting host fitness through auxin signaling, providing novel insights into the application of cross-kingdom SynComs in medicinal plant research.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128312"},"PeriodicalIF":6.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organoids in host pathology: Unveiling microbial-induced therapeutic responses","authors":"Linhan Sun ,&nbsp;Yangmiao Duan ,&nbsp;Qingzhan Lan ,&nbsp;Yunshan Wang ,&nbsp;Alice S.T. Wong ,&nbsp;Yang Ni ,&nbsp;Duanrui Liu","doi":"10.1016/j.micres.2025.128313","DOIUrl":"10.1016/j.micres.2025.128313","url":null,"abstract":"<div><div>Organoids are three-dimensional (3D) cultures derived from stem cells that replicate the complex structure and function of human tissues. They provide a physiologically relevant platform for studying microbial-host interactions, offering insights into pathogen-induced host lesions and therapeutic responses. By mimicking the microenvironment of organs such as the intestine, lung, and brain, organoids were applied in microbial pathogenesis, immune responses, and drug efficacy. Despite challenges in simulating immune dynamics and chronic infections, advancements in vascularization, immune component integration, and 3D bioprinting hold promise for breakthroughs in infectious disease research and precision medicine. This review highlights the transformative potential of organoids in understanding the mechanisms of pathogen infection and developing targeted therapies.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128313"},"PeriodicalIF":6.9,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C28-aldehyde (n-octacosanal) modulates stage-specific temporal expression of effector genes in the wheat powdery mildew fungus","authors":"Mo Zhu ,&nbsp;Wanwan Zhang ,&nbsp;Fuhai Zhang ,&nbsp;Xiao Duan ,&nbsp;Zongbo Qiu ,&nbsp;Sujing Zhao ,&nbsp;Shiqiang Gao ,&nbsp;Fei He","doi":"10.1016/j.micres.2025.128311","DOIUrl":"10.1016/j.micres.2025.128311","url":null,"abstract":"<div><div>The prepenetration processes of the wheat powdery mildew fungus, <em>Blumeria graminis</em> f. sp. <em>tritici</em> (Bgt), are triggered by C₂₈ aldehyde (<em>n</em>-octacosanal), a component of cuticular waxes. Despite being the most severe crop disease worldwide, the underlying molecular mechanisms of the prepenetration processes remain obscure. Utilizing a Formvar®-based <em>in vitro</em> system, transcriptomes of Bgt conidia impacted by <em>n</em>-octacosanal were profiled without the effects from plant host. A total of 1354 differentially expressed genes were identified between <em>n</em>-octacosanal- and <em>n</em>-octacosane (non-chemical signal)-treated conidia. The expression of effectors, transcription factors, and HOG-MAPK pathways is specifically regulated by <em>n</em>-octacosanal in a developmental stage-dependent manner. Among them, 25 effectors and three transcription factors, including COD 1, VEA, and CreA, were highly expressed at all stages. While some genes of the HOG-MAPK pathway were significantly upregulated during conidial growth, other genes were downregulated. These results revealed that C₂₈ aldehyde-triggered Bgt conidial prepenetration in the plant host might be achieved by activating specific transcription factors and differentially regulating the HOG-MAPK pathway. The genes detected by our gene expression analysis may be crucial for successful infection by Bgt and thus serve as candidates for future functional analysis of the molecular mechanisms of conidia development in powdery mildew. These findings provide new insights into the chemical-signal-orchestrated development of an important phytopathogenic fungus and will potentially support efforts for the control and management of fungal diseases in wheat.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128311"},"PeriodicalIF":6.9,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pirin, a redox-sensitive modulator of beta-oxidation, generates hydroxyl radicals and interacts with CatR, the transcriptional repressor of the major vegetative catalase gene in Streptomyces","authors":"Matteo Calcagnile ,&nbsp;Fabrizio Damiano ,&nbsp;Adelfia Talà ,&nbsp;Pietro Alifano","doi":"10.1016/j.micres.2025.128310","DOIUrl":"10.1016/j.micres.2025.128310","url":null,"abstract":"<div><div>Pirins are iron-containing proteins conserved throughout evolution, which have been implicated in diverse cellular processes, mostly associated with stress. In prokaryotes, Pirins are present in many taxonomic groups and can be present in multiple copies, and only a few of these proteins have been studied. In <em>Streptomyces ambofaciens</em> a Pirin-like protein, PirA, is a redox-sensitive negative modulator of AcdB, a very long-chain acyl-CoA dehydrogenase (vLCAD), which catalyzes the first committed step of the beta-oxidation pathway. In this study, we first classified the Pirins in different prokaryotic and non-prokaryotic taxa, and we found strong connections between the occurrence of Pirins and aerobic energy metabolism. We then studied whether the presence of Pirins is connected to the regulation of antioxidant systems, after observing that a <em>pirA</em>-defective mutant of <em>S. ambofaciens</em> accumulated large amounts of H₂O₂ during the vegetative growth. <em>In vitro</em> experiments suggested that the accumulation of H₂O₂ in the <em>pirA</em> mutant could be partially due to an increased vLCAD activity of AcdB, which releases H₂O₂ as a byproduct of the reaction. However, the reduction in catalase and alkylhydroperoxidase expression levels in the <em>pirA</em> mutant, despite the increased amount of intracellular H₂O₂, also indicated a dysregulation of these antioxidant systems. Indeed, the gene <em>catA</em> encoding the major vegetative catalase and the adjacent regulatory gene <em>catR</em> were down-regulated in the <em>pirA</em> mutant. In Gram-positive bacteria, CatR/PerR family regulators repress transcription of genes coding for the major vegetative catalase. These repressors are irreversible inactivated by hydroxyl radicals and detach from the DNA, which leads to de-repression of catalase expression. We found that PirA generates hydroxyl radicals after reacting with H₂O₂, and binds CatR <em>in vitro</em>, suggesting that by generating hydroxyl radicals in response to H₂O₂ exposure, PirA could promote CatR inactivation allowing <em>catA</em> transcription.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128310"},"PeriodicalIF":6.9,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144852096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the interactions of gut microbiome and metabolites in polycystic ovary syndrome: a review","authors":"Jueyu Li ,&nbsp;Yuqi Jia ,&nbsp;Haojie Xu ,&nbsp;Hao Bing ,&nbsp;Qixian Ling ,&nbsp;Sihan Wang ,&nbsp;Yanan Wang ,&nbsp;Xianhua Zhang ,&nbsp;Libo Zhao","doi":"10.1016/j.micres.2025.128309","DOIUrl":"10.1016/j.micres.2025.128309","url":null,"abstract":"<div><div>Polycystic ovary syndrome (PCOS) is a prevalent endocrine metabolic disorder significantly impacting female health. While its etiology remains incompletely understood, emerging evidence highlights the gut microbiome as a key modulator, affecting not only the gut but also physiology beyond it. Many studies have been committed to exploring the role played by the gut microbiome and have discovered that gut microbiome is commonly dysregulated in PCOS patients. In this review, we offered an introduction to PCOS and summarized the high-quality research in recent years to elucidate the complex interactions between endogenous metabolites with the gut microbiome in PCOS. Furthermore, we also discuss microbiome-targeting therapeutic strategies. By integrating current knowledge on the gut microbiome-metabolite axis, this review aims to provide novel insights and perspectives for future research and potential management of PCOS.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"301 ","pages":"Article 128309"},"PeriodicalIF":6.9,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144864130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}