Microbiological research最新文献

{"title":"Gut microbiota modulates neurotransmitter and gut-brain signaling","authors":"Shiyan Qu ,&nbsp;Zijin Yu ,&nbsp;Yaxuan Zhou ,&nbsp;Shiyi Wang ,&nbsp;Minqi Jia ,&nbsp;Ti Chen ,&nbsp;Xiaojie Zhang","doi":"10.1016/j.micres.2024.127858","DOIUrl":"10.1016/j.micres.2024.127858","url":null,"abstract":"<div><p>Neurotransmitters, including 5-hydroxytryptamine (5-HT), dopamine (DA), gamma-aminobutyric acid (GABA), and glutamate, are essential transductors in the Gut-Brain Axis (GBA), playing critical roles both peripherally and centrally. Accumulating evidence suggests that the gut microbiota modulates intestinal neurotransmitter metabolism and gut-to-brain signaling, shedding light on the crucial role of the gut microbiota in brain function and the pathogenesis of various neuropsychiatric diseases, such as major depression disorder (MDD), anxiety, addiction and Parkinson's disease (PD). Despite the exciting findings, the mechanisms underlying the modulation of neurotransmitter metabolism and function by the gut microbiota are still being elucidated. In this review, we aim to provide a comprehensive overview of the existing knowledge about the role of the gut microbiota in neurotransmitter metabolism and function in animal and clinical experiments. Moreover, we will discuss the potential mechanisms through which gut microbiota-derived neurotransmitters contribute to the pathogenesis of neuropsychiatric diseases, thus highlighting a novel therapeutic target for these conditions.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127858"},"PeriodicalIF":6.1,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervicovaginal microbiome, high-risk HPV infection and cervical cancer: Mechanisms and therapeutic potential","authors":"Roujie Huang ,&nbsp;Zimo Liu ,&nbsp;Tianshu Sun ,&nbsp;Lan Zhu","doi":"10.1016/j.micres.2024.127857","DOIUrl":"10.1016/j.micres.2024.127857","url":null,"abstract":"<div><p>The microbiota in the female genital tract is an intricate assembly of diverse aerobic, anaerobic, and microaerophilic microorganisms, which share the space within the reproductive tract and engage in complex interactions. Microbiome dysbiosis may disrupt the symbiotic relationship between the host and microorganisms and play a pivotal role in the pathogenesis of various diseases, including its involvement in the establishment of human papillomavirus (HPV)-associated cervical cancer (CC). Interventions to restore microbiota homeostasis (e.g., probiotics) and bacterial-vector HPV therapeutic vaccines have been reported to be potentially effective in clearing HPV infection and ameliorating cytological abnormalities. In this review, we place emphasis on elucidating the alterations within the cervical–vaginal microbiota as well as the intratumoral microbiota in the context of high-risk HPV (HR-HPV) infection and its subsequent progression to cervical intraepithelial neoplasia/CC. Furthermore, we explore the mechanisms by which these microbial communities exert potential pathogenic or protective effects, including modulating genital inflammation and immune responses, affecting HR-HPV oncogene expression and oncoprotein production, regulating oxidative stress and deoxyribonucleic acid (DNA) damage, and inducing metabolic rewiring. Lastly, we summarize the latest evidence in human trials regarding the efficacy of probiotics, prebiotics and probiotic-vector HPV therapeutic vaccines. This review aims to foster a deeper understanding of the role of the microbiota in HR-HPV infection-related cervix cancer development, and further provide a theoretical basis for the development of preventive and therapeutic strategies based on microbial modulation.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127857"},"PeriodicalIF":6.1,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002581/pdfft?md5=512d9986f5f6bdf21960bfbef1a95b3c&pid=1-s2.0-S0944501324002581-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics analysis reveals the effects of three application modes of plant growth promoting microbes biofertilizer on potato (Solanum tuberosum L.) growth under alkaline loess conditions","authors":"Yuan Tian ,&nbsp;Yang Liu ,&nbsp;Liang Yue ,&nbsp;Xia Zhao ,&nbsp;Qin Zhou ,&nbsp;Constantine Uwaremwe ,&nbsp;Yun Wang ,&nbsp;Gaofeng Chen ,&nbsp;Yuexia Sha ,&nbsp;Yubao Zhang ,&nbsp;Ruoyu Wang","doi":"10.1016/j.micres.2024.127855","DOIUrl":"10.1016/j.micres.2024.127855","url":null,"abstract":"<div><p>Potato is an important crop due to its high contents of starch, protein, and various vitamins and minerals. Biofertilizers are composed of plant growth promoting microbes (PGPMs) which are essential for improving the growth and resistance of potato. However, little information has focused on the modes of inoculation of biofertilizers on plant growth and microecology. This study aims to reveal the response mechanism of the potato to three modes of inoculation of biofertilizers all containing PGPM <em>Bacillus amyloliquefaciens</em> EZ99, i.e. scattered mode of 5 kg/ha biofertilizer (M5), soaking seed tubers with dissolved 5 kg/ha biofertilizer (MZG), and scattered mode of 3 kg/ha biofertilizer + 2 kg/ha sucrose (MY34) in alkaline loess field through multi-omics analysis of transcriptome, metabolome and microbiome. The physiological result revealed that two application modes of equal amount of biofertilizer M5 and MZG significantly improved the growth and yield of potatoes. Furthermore, the transcriptome of potato exhibited sets of differentially expressed genes enriched in photosynthesis, sugar metabolism, and phenylpropanoid biosynthesis among the three modes, with the M5 mode exhibiting overall up-regulation of 828 genes. Based on the untargeted metabolomic analysis of potato tuber, M5 mode significantly accumulated sucrose, while MZG and MY34 mode significantly accumulated the stress metabolites euchrenone b6 and mannobiose, respectively. Besides, the microbial structure of potato rhizosphere showed that the diversity of bacteria and fungi was similar in all soils, but their abundances varied significantly. Specifically, beneficial <em>Penicillium</em> was enriched in M5 and MZG soils<em>,</em> whereas MY34 soil accumulated potential pathogens <em>Plectosphaerella</em> and saccharophilic <em>Mortierella</em>. Collectively, these e findings highlight that MZG is the most effective mode to promote potato growth and stimulate rhizosphere effect. The present study not only encourages sustainable agriculture through agroecological practices, but also provides broad prospects for the application of PGPM biofertilizer in staple foods.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127855"},"PeriodicalIF":6.1,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A bud's life: Metabarcoding analysis to characterise hazelnut big buds microbiome biodiversity","authors":"Silvia Turco ,&nbsp;Federico Brugneti ,&nbsp;Irene Giubilei ,&nbsp;Cristian Silvestri ,&nbsp;Miloš Petrović ,&nbsp;Mounira Inas Drais ,&nbsp;Valerio Cristofori ,&nbsp;Stefano Speranza ,&nbsp;Angelo Mazzaglia ,&nbsp;Mario Contarini ,&nbsp;Luca Rossini","doi":"10.1016/j.micres.2024.127851","DOIUrl":"10.1016/j.micres.2024.127851","url":null,"abstract":"<div><p>Despite <em>Corylus avellana</em> L. being an economically important shrub species known for its resilience to adverse environmental conditions, it constantly faces attacks from a plethora of biotic entities. Among these, the mite pest <em>Phytoptus avellanae</em> is gaining importance, causing economic losses every year. This mite colonises the new generative and vegetative buds, leading them to become swollen and reddish, and drastically reducing hazelnut production. The biology behind gall formation is still poorly understood. This study provides a qualitative and quantitative description of the microbiome in both healthy and infested buds of two economically important hazelnut cultivars through metabarcoding of fungal ITS and bacterial 16 S. Potentially pathogenic genera such as <em>Fusarium</em> and <em>Pseudomonas</em> were predominant in the infested buds, along with the obligate intracellular bacterial genus <em>Wolbachia. Akanthomyces muscarius</em> was instead isolated from culture-based methods only from the infested buds. These findings could improve the understanding of gall ecology, supporting the management of mite populations, and they could also serve as a milestone for further studies on low-impact, monitoring-driven, and genetically targeted control strategies.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127851"},"PeriodicalIF":6.1,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002520/pdfft?md5=b98ddf6fcafeaa1a675a920637c9c53d&pid=1-s2.0-S0944501324002520-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacillus paralicheniformis, an acetate-producing probiotic, alleviates ulcerative colitis via protecting the intestinal barrier and regulating the NLRP3 inflammasome","authors":"Nini Dai ,&nbsp;Xinting Yang ,&nbsp;Peilong Pan ,&nbsp;Guanghui Zhang ,&nbsp;Kangliang Sheng ,&nbsp;Jingmin Wang ,&nbsp;Xiao Liang ,&nbsp;Yongzhong Wang","doi":"10.1016/j.micres.2024.127856","DOIUrl":"10.1016/j.micres.2024.127856","url":null,"abstract":"<div><p>Ulcerative colitis (UC) presents a challenging scenario in digestive health, characterized by recurrent inflammation that is often hard to manage. Bacteria capable of producing short-chain fatty acids (SCFAs) play a pivotal role in mitigating UC symptoms, rendering them promising candidates for probiotic therapy. In this investigation, we assessed the impact of <em>Bacillus paralicheniformis</em> HMPM220325 on dextran sodium sulfate (DSS)-induced UC in mice. Genomic analysis of the strain revealed the presence of protease genes associated with acetate and butyrate synthesis, with acetic acid detected in its fermentation broth. Administration of <em>B. paralicheniformis</em> HMPM220325 to UC mice ameliorated pathological manifestations of the condition and restored intestinal barrier function. Furthermore, <em>B. paralicheniformis</em> HMPM220325 suppressed the activation of the NLRP3 inflammasome signaling pathway and modulated the composition of the intestinal microbiota. These findings shed significant light on the potential of <em>B. paralicheniformis</em> as a probiotic candidate, offering a novel avenue for the prevention and therapeutic intervention of colitis.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127856"},"PeriodicalIF":6.1,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141786289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and eco-physiological responses of soil-borne lead (Pb2+)-resistant bacteria for bioremediation and plant growth promotion under lead stress","authors":"Priyanka Pal ,&nbsp;Krishnendu Pramanik ,&nbsp;Sudip Kumar Ghosh ,&nbsp;Sayanta Mondal ,&nbsp;Tanushree Mondal ,&nbsp;Tithi Soren ,&nbsp;Tushar Kanti Maiti","doi":"10.1016/j.micres.2024.127831","DOIUrl":"10.1016/j.micres.2024.127831","url":null,"abstract":"<div><p>Lead (Pb) is the 2^nd known portentous hazardous substance after arsenic (As). Being highly noxious, widespread, non-biodegradable, prolonged environmental presence, and increasing accumulation, particularly in arable land, Pb pollution has become a serious global health concern requiring urgent remediation. Soil-borne, indigenous microbes from Pb-polluted sites have evolved diverse resistance strategies, involving biosorption, bioprecipitation, biomineralization, biotransformation, and efflux mechanisms, under continuous exposure to Pb in human-impacted surroundings. These strategies employ a wide range of functional bioligands to capture Pb and render it inaccessible for leaching. Recent breakthroughs in molecular technology and understanding of lead resistance mechanisms offer the potential for utilizing microbes as biological tools in environmental risk assessment. Leveraging the specific affinity and sensitivity of bacterial regulators to Pb²⁺ ions, numerous lead biosensors have been designed and deployed worldwide to monitor Pb bioavailability in contaminated sites, even at trace levels. Besides, the ongoing degradation of croplands due to Pb pollution poses a significant challenge to meet the escalating global food demands. The accumulation of Pb in plant tissues jeopardizes both food safety and security while severely impacting plant growth. Exploring Pb-resistant plant growth-promoting rhizobacteria (PGPR) presents a promising sustainable approach to agricultural practices. The active associations of PGPR with host plants have shown enhancements in plant biomass and stress alleviation under Pb influence. They thus serve a dual purpose for plants grown in Pb-contaminated areas. This review aims to offer a comprehensive understanding of the role played by Pb-resistant soil-borne indigenous bacteria in expediting bioremediation and improving the growth of Pb-challenged plants essential for potential field application, thus broadening prospects for future research and development.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127831"},"PeriodicalIF":6.1,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141783205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine ameliorates vascular dysfunction by downregulating TMAO-endoplasmic reticulum stress pathway via gut microbiota in hypertension","authors":"Zhichao Wang ,&nbsp;Yijia Shao ,&nbsp;Fang Wu ,&nbsp;Dangu Luo ,&nbsp;Guoyifan He ,&nbsp;Jianwen Liang ,&nbsp;Xiaoqing Quan ,&nbsp;Xiehui Chen ,&nbsp;Wenhao Xia ,&nbsp;Ye Chen ,&nbsp;Yue Liu ,&nbsp;Long Chen","doi":"10.1016/j.micres.2024.127824","DOIUrl":"10.1016/j.micres.2024.127824","url":null,"abstract":"<div><p>The gut microbial metabolite trimethylamine N-oxide (TMAO) is regarded as a novel risk factor for hypertension. Berberine (BBR) exerts cardiovascular protective effects by regulating the gut microbiota-metabolite production pathway. However, whether and how BBR alleviates TMAO-induced vascular dysfunction in hypertension remains unclear. In the present study, we observed that plasma TMAO and related bacterial abundance were significantly elevated and negatively correlated with vascular function in 86 hypertensive patients compared with 46 normotensive controls. TMAO activated endoplasmic reticulum stress (ERS) signaling pathway to promote endothelial cell dysfunction and apoptosis in vitro. BBR (100, 200 mg · kg⁻¹ ·d⁻¹) for 4 weeks ameliorates TMAO-induced vascular dysfunction and ERS activation in a choline-angiotensin II hypertensive mouse model. We found that plasma TMAO levels in 15 hypertensive patients treated with BBR (0.4 g, tid) were reduced by 8.8 % and 16.7 % at months 1 and 3, respectively, compared with pretreatment baseline. The oral BBR treatment also improved vascular function and lowered blood pressure. Faecal 16 S rDNA showed that BBR altered the gut bacterial composition and reduced the abundance of CutC/D bacteria in hypertensive mice and patients. In vitro bacterial cultures and enzyme reaction systems indicated that BBR inhibited the biosynthesis of TMAO precursor in the gut microbiota by binding to and inhibiting the activity of CutC/D enzyme. Our results indicate that BBR improve vascular dysfunction at least partially by decreasing TMAO via regulation of the gut microbiota in hypertension.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127824"},"PeriodicalIF":6.1,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0944501324002258/pdfft?md5=48f3c1de219da59376433e3d27a0e957&pid=1-s2.0-S0944501324002258-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small RNA-regulated expression of efflux pump affects tigecycline resistance and heteroresistance in clinical isolates of Klebsiella pneumoniae","authors":"Yuqiao Han ,&nbsp;Yilin Xiong ,&nbsp;Mengyao Wang ,&nbsp;Jia Wang ,&nbsp;Tao Song ,&nbsp;Jing Yu ,&nbsp;Jia Hu ,&nbsp;Zinan Zhao ,&nbsp;Ming Li ,&nbsp;Ying Li ,&nbsp;Yang Chen","doi":"10.1016/j.micres.2024.127825","DOIUrl":"10.1016/j.micres.2024.127825","url":null,"abstract":"<div><p>Tigecycline and the newly Food and Drug Administration-approved tetracyclines, including eravacycline and omadacycline, are regarded as last-resort treatments for multidrug-resistant Enterobacterales<em>.</em> However, tigecycline resistance in <em>Klebsiella pneumoniae</em> has increased, especially the underlying mechanism of heteroresistance is unclear. This study aimed to elucidate the mechanisms underlying tigecycline resistance and heteroresistance in clinical <em>K. pneumoniae</em> isolates. A total of 153 clinical <em>K. pneumoniae</em> isolates were collected, and identified 15 tigecycline-resistant and three tigecycline-heteroresistant isolates using broth microdilution and population analysis profile methods, respectively. Total RNAs from <em>K. pneumoniae</em> ATCC13883 and the laboratory-induced tigecycline-resistant strain were extracted and sequenced on an Illumina platform. Differentially expressed genes and regulatory small RNAs (sRNAs) were analyzed and validated in clinical isolates of <em>K. pneumoniae</em> using quantitative real-time PCR. RNA sequencing results showed that <em>mdtABC</em> efflux pump genes were significantly upregulated in the tigecycline-resistant strains. Overexpression of <em>mdtABC</em> was observed in a clinical <em>K. pneumoniae</em> isolate, which increased tigecycline minimum inhibitory concentrations (MICs) and was involved in tigecycline heteroresistance. Sequencing analysis of sRNA demonstrated that candidate sRNA-120 directly interacted with the <em>mdtABC</em> operon and was downregulated in tigecycline-resistant strains. We generated an sRNA-120 deletion mutation strain and a complemented strain of <em>K. pneumoniae.</em> The sRNA-120 deletion strain displayed increased mRNA levels of <em>mdtA, mdtB,</em> and <em>mdtC</em> and an increase in MICs of tigecycline. The complemented strain of sRNA-120 restored the mRNA levels of these genes and the susceptibility to tigecycline. RNA antisense purification and parallel reaction monitoring mass spectrometry were performed to verify the interactions between sRNA-120 and <em>mdtABC</em>. Collectively, our study highlights that the post-transcriptional repression of <em>mdtABC</em> through sRNA-120 may provide an additional layer of efflux pump gene expression control, which is important for resistance and heteroresistance in clinical <em>K. pneumoniae</em> isolates.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127825"},"PeriodicalIF":6.1,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sphingomonas sp. Hbc-6 alters Arabidopsis metabolites to improve plant growth and drought resistance by manipulating the microbiome","authors":"Fang Wang ,&nbsp;Mingyue Jia ,&nbsp;Kun Li ,&nbsp;Yafang Cui ,&nbsp;Lizhe An ,&nbsp;Hongmei Sheng","doi":"10.1016/j.micres.2024.127852","DOIUrl":"10.1016/j.micres.2024.127852","url":null,"abstract":"<div><p>Drought significantly affects crop productivity and poses a considerable threat to agricultural ecosystems. Plant growth-promoting bacteria (PGPB) and plant microbiome play important roles in improving drought resistance and plant performance. However, the response of the rhizosphere microbiota to PGPB during the development of plants and the interaction between inoculum, microbiota, and plants under drought stress remain to be explored. In the present study, we used culturomic, microbiomic, and metabonomic analyses to uncover the mechanisms by which <em>Sphingomonas</em> sp. Hbc-6, a PGPB, promotes <em>Arabidopsis</em> growth and enhances drought resistance. We found that the rhizosphere microbiome assembly was interactively influenced by developmental stage, Hbc-6, and drought; the bacterial composition exhibited three patterns of shifts with developmental stage: resilience, increase, and decrease. Drought diminished microbial diversity and richness, whereas Hbc-6 increased microbial diversity and helped plants recruit specific beneficial bacterial taxa at each developmental stage, particularly during the bolting stage. Some microorganisms enriched by Hbc-6 had the potential to promote carbon and nitrogen cycling processes, and 86.79 % of the isolated strains exhibited PGP characteristics (for example <em>Pseudomonas</em> sp. TA9). They jointly regulated plant physiological metabolism (i.e., upregulated drought resistant-facilitating substances and reduced harmful substances), thereby stimulating the growth of <em>Arabidopsis</em> and increasing plant biomass under drought stress conditions. Collectively, these results indicate that Hbc-6 mediates plant growth and drought resistance by affecting the microbiome. The study thus provides novel insights and strain resources for drought-resistant, high-yielding crop cultivation and breeding.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127852"},"PeriodicalIF":6.1,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the journey: A comprehensive review of vaccine development against Klebsiella pneumoniae","authors":"Bruno Douradinha","doi":"10.1016/j.micres.2024.127837","DOIUrl":"10.1016/j.micres.2024.127837","url":null,"abstract":"<div><p><em>Klebsiella pneumoniae</em>, a prominent nosocomial pathogen, poses a critical global health threat due to its multidrug-resistant (MDR) and hypervirulent strains. This comprehensive review focuses into the complex approaches undertaken in the development of vaccines against <em>K. pneumoniae</em>. Traditional methods, such as whole-cell and ribosomal-based vaccines, are compared with modern strategies, including DNA and mRNA vaccines, and extracellular vesicles (EVs), among others. Each method presents unique advantages and challenges, emphasising the complexity of developing an effective vaccine against this pathogen. Significant advancements in computational tools and artificial intelligence (AI) have revolutionised antigen identification and vaccine design, enhancing the precision and efficiency of developing multiepitope-based vaccines. The review also highlights the potential of glycomics and immunoinformatics in identifying key antigenic components and elucidating immune evasion mechanisms employed by <em>K. pneumoniae</em>. Despite progress, challenges remain in ensuring the safety, efficacy, and manufacturability of these vaccines. Notably, EVs demonstrate promise due to their intrinsic adjuvant properties and ability to elicit robust immune responses, although concerns regarding inflammation and antigen variability persist. This review provides a critical overview of the current landscape of <em>K. pneumoniae</em> vaccine development, stressing the need for continued innovation and interdisciplinary collaboration to address this pressing public health issue. The integration of advanced computational methods and AI holds the potential to accelerate the development of effective immunotherapies, paving the way for novel vaccines against MDR <em>K. pneumoniae</em>.</p></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"287 ","pages":"Article 127837"},"PeriodicalIF":6.1,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141766710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}