Microbiological research最新文献_第10页

Temperate bacteriophage SapYZUs7 alters Staphylococcus aureus fitness balance by regulating expression of phage resistance, virulence and antimicrobial resistance gene 温带噬菌体SapYZUs7通过调控噬菌体耐药性、毒力和耐药基因的表达改变金黄色葡萄球菌的适应性平衡。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-26 DOI: 10.1016/j.micres.2024.128040

Wenyuan Zhou , Yajie Li , Yuhong Wu , Weicheng Hu , Wenjuan Li , Aiping Deng , Yeling Han , Guoqiang Zhu , Zhenquan Yang

{"title":"Temperate bacteriophage SapYZUs7 alters Staphylococcus aureus fitness balance by regulating expression of phage resistance, virulence and antimicrobial resistance gene","authors":"Wenyuan Zhou , Yajie Li , Yuhong Wu , Weicheng Hu , Wenjuan Li , Aiping Deng , Yeling Han , Guoqiang Zhu , Zhenquan Yang","doi":"10.1016/j.micres.2024.128040","DOIUrl":"10.1016/j.micres.2024.128040","url":null,"abstract":"<div><div>Temperate bacteriophages are crucial for maintaining the pathogenicity and fitness of <em>S. aureus</em>, which also show promise as a biocontrol agent for <em>S. aureus</em>. However, the fitness benefit and cost of lysogeny by <em>S. aureus</em> temperate phages and their underlying mechanisms remain unexplored. In this study, phage resistance, virulence, antimicrobial resistance (AMR), transcriptome, and metabolome of phage SapYZUs7 lysogenic and non-lysogenic <em>S. aureus</em> strains were compared. Whole-genome analysis revealed that SapYZUs7 harbouring <em>smaII</em> associated with a single-protein MazF-like antiphage system could be integrated into the genome of <em>S. aureus</em> isolates. Notably, lysogenic <em>S. aureus</em> exhibited higher phage resistance, a lower growth rate, and inhibited metabolic activity compared to the parental strains, indicating interference of phage reproduction by <em>smaII</em>. Moreover, prophages carrying <em>smaII</em> are widely distributed across <em>S. aureus</em> and harboured other virulence factor (VF) and AMR genes. Besides, the SapYZUs7-integration increased phagocytosis resistance but decreased adhesion, biofilm formation, and AMR. The combined use of SapYZUs7 and antibiotics exhibited a better bactericidal effect than SapYZUs7 or the antibiotics alone. Consistently, integrated omics analysis suggested that SapYZUs7-lysogeny downregulated multiple VF and AMR genes. Our analysis suggests that <em>Sma</em>II drives mutualistic phage-host interactions through lysogenic conversion. The fitness cost of SapYZUs7-integration is the downregulated expression of VF and AMR genes, serving as an alternative candidate as a biocontrol agent for methicillin-resistant <em>S</em>. <em>aureus</em> and multidrug-resistant <em>S</em>. <em>aureus</em>.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128040"},"PeriodicalIF":6.1,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stealth in non-tuberculous mycobacteria: clever challengers to the immune system 非结核分枝杆菌的隐身性：免疫系统的聪明挑战者。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-25 DOI: 10.1016/j.micres.2024.128039

Zhenghao Wang , Xiurong Sun , Yuli Lin , Yurong Fu , Zhengjun Yi

{"title":"Stealth in non-tuberculous mycobacteria: clever challengers to the immune system","authors":"Zhenghao Wang , Xiurong Sun , Yuli Lin , Yurong Fu , Zhengjun Yi","doi":"10.1016/j.micres.2024.128039","DOIUrl":"10.1016/j.micres.2024.128039","url":null,"abstract":"<div><div><em>Non-tuberculous Mycobacteria (</em>NTM<em>)</em> are found extensively in various environments, yet most are non-pathogenic. Only a limited number of these organisms can cause various infections, including those affecting the lungs, skin, and central nervous system, particularly when the host's autoimmune function is compromised. Among these, Non-tuberculous Mycobacteria Pulmonary Diseases (NTM-PD) are the most prevalent. Currently, there is a lack of effective treatments and preventive measures for NTM infections. This article aims to deepen the comprehension of the pathogenic mechanisms linked to NTM and to formulate new intervention strategies by synthesizing current research and detailing the different tactics used by NTM to avoid elimination by the host's immune response. These intricate mechanisms not only affect the innate immune response but also successfully oppose the adaptive immune response, establishing persistent infections within the host. This includes effects on the functions of macrophages, neutrophils, dendritic cells, and T lymphocytes, as well as modulation of cytokine production. The article particularly emphasizes the survival strategies of NTM within macrophages, such as inhibiting phagosome maturation and acidification, resisting intracellular killing mechanisms, and interfering with autophagy and cell death pathways. This review aims to deepen the understanding of NTM's immune evasion mechanisms, thereby facilitating efforts to inhibit its proliferation and spread within the host, ultimately providing new methods and strategies for NTM-related treatments.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128039"},"PeriodicalIF":6.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142927222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphorylation of the prokaryotic histone-like protein H-NS modulates bacterial virulence in Salmonella Typhimurium 原核组蛋白样蛋白H-NS的磷酸化调节鼠伤寒沙门菌的细菌毒力。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-25 DOI: 10.1016/j.micres.2024.128041

Ying Wang , Jinli Ge , Wei Xian , Zhiheng Tang , Baoshuai Xue , Jingchen Yu , Yu-Feng Yao , Huwei Liu , Jiazhang Qiu , Xiaoyun Liu

{"title":"Phosphorylation of the prokaryotic histone-like protein H-NS modulates bacterial virulence in Salmonella Typhimurium","authors":"Ying Wang , Jinli Ge , Wei Xian , Zhiheng Tang , Baoshuai Xue , Jingchen Yu , Yu-Feng Yao , Huwei Liu , Jiazhang Qiu , Xiaoyun Liu","doi":"10.1016/j.micres.2024.128041","DOIUrl":"10.1016/j.micres.2024.128041","url":null,"abstract":"<div><div>H-NS is a prokaryotic histone-like protein that binds to bacterial chromosomal DNA with important regulatory roles in gene expression. Unlike histone proteins, hitherto post-translational modifications of H-NS are still largely uncharacterized, especially in bacterial pathogens. <em>Salmonella</em> Typhimurium is a primary enteric pathogen and its virulence is mainly dependent on specialized type III secretion systems (T3SSs), which were evolutionarily acquired via horizontal gene transfer. Previous studies have shown that H-NS plays a critical role in silencing foreign T3SS genes. Here, we found that H-NS is phosphorylated at multiple residues in <em>S</em>. Typhimurium, including S45, Y61, S78, S84, T86, and T106. Notably, we demonstrated that phosphorylation of H-NS S78 promotes its dissociation from DNA via a mechanism dependent on dimer formation, thereby leading to transcriptional activation of target genes. Functionally, phosphoryl-H-NS contributes to the expression of T3SS-associated proteins and hence increases bacterial virulence during infection. Therefore, our study reveals a novel mechanism by which covalent modifications of prokaryotic histone-like proteins regulate bacterial virulence of an important human pathogen.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128041"},"PeriodicalIF":6.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142907141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting ClpP: Unlocking a novel therapeutic approach of isochlorogenic acid A for methicillin-resistant Staphylococcus aureus-infected osteomyelitis 靶向ClpP：开启异绿原酸a治疗耐甲氧西林金黄色葡萄球菌感染骨髓炎的新途径

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-24 DOI: 10.1016/j.micres.2024.128042

Yueying Wang , Li Wang , Dongbin Guo , Xinyao Liu , Yueshan Xu , Rong Wang , Yun Sun , Quan Liu , Jiyu Guan , Da Liu , Bingmei Wang , Yicheng Zhao , Ming Yan

{"title":"Targeting ClpP: Unlocking a novel therapeutic approach of isochlorogenic acid A for methicillin-resistant Staphylococcus aureus-infected osteomyelitis","authors":"Yueying Wang , Li Wang , Dongbin Guo , Xinyao Liu , Yueshan Xu , Rong Wang , Yun Sun , Quan Liu , Jiyu Guan , Da Liu , Bingmei Wang , Yicheng Zhao , Ming Yan","doi":"10.1016/j.micres.2024.128042","DOIUrl":"10.1016/j.micres.2024.128042","url":null,"abstract":"<div><div>A medical predicament has led to extensive drug resistance in methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), and the complexity of treatment has increased exponentially with the induction of osteomyelitis. In view of the severe situation and the potential of bacterial antivirulence strategies, this study focused on the key virulence factor caseinolytic protease (ClpP) of <em>S. aureus</em> to identify new strategies against MRSA-induced osteomyelitis. As the main protein \"quality control\" system of <em>S. aureus</em>, ClpP is indispensable for coordinating drug resistance, regulating adhesion, and acting on numerous virulence targets. Through fluorescence resonance energy transfer (FRET), we successfully identified isochlorogenic acid A (I-A), a polyphenol derivative, as an efficient inhibitor of ClpP, with an IC<sub>50</sub> value of 24.89 μg/mL. Further analysis revealed that I-A can effectively inhibit the expression of virulence factors of MRSA and significantly reduce its adhesion to fibrinogen. Molecular docking revealed the potential binding sites of ClpP and I-A, namely, ILE-81, LYS-109, GLU-156, ARG-157, and GLY-184. At the cellular level, I-A can alleviate the death and increased secretion of inflammatory factors caused by MRSA USA300 in MC3T3-E1 cells. Moreover, it downregulates the activity of ClpP and reduces the response of bacteria to environmental stress. <em>In vivo</em> experiments have confirmed that I-A shows significant efficacy in both rat osteomyelitis models and <em>Galleria mellonella</em> infection models. This study provides new insights into the field of treatment strategies targeting virulence and provides a solid foundation for further exploration of the potential of I-A in combating drug-resistant <em>S. aureus</em>.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128042"},"PeriodicalIF":6.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploitative stress within Bacillus subtilis biofilm determines the spatial distribution of pleomorphic cells 枯草芽孢杆菌生物膜内的开发应激决定了多形性细胞的空间分布。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-22 DOI: 10.1016/j.micres.2024.128034

Sumon Dey , Ankit Kumar Nayak , Hema Rajaram , Surajit Das

{"title":"Exploitative stress within Bacillus subtilis biofilm determines the spatial distribution of pleomorphic cells","authors":"Sumon Dey , Ankit Kumar Nayak , Hema Rajaram , Surajit Das","doi":"10.1016/j.micres.2024.128034","DOIUrl":"10.1016/j.micres.2024.128034","url":null,"abstract":"<div><div>Bacteria commonly live in a spatially organized biofilm assemblage. The metabolic activity inside the biofilm leads to segmented physiological microenvironments. In nature, bacteria possess several pleomorphic forms to withstand certain ecological alterations. We hypothesized that pleomorphism also exists within the biofilm, which can be considered as the fundamental niche for bacteria. We report a distinct pattern of cell size variation throughout the biofilm of <em>Bacillus subtilis</em>. Cell size heterogeneity was observed in biofilm development, wherein the frequency of long cells is higher in outer regions, whereas lower in inner regions. Moreover, compared to planktonic cells, bacteria in the biofilm mode reduce their geometric ratio from 8.34 to 3.69 and 2.65 in the outer and inner regions, respectively. There were no significant differences observed in nutrient diffusion from the outer to the inner region, and more than 73 % of cells in the inner region were viable. However, the inner and middle regions were more acidic than the outer of the biofilm. Conclusively, growth rate-independent cell size reduction at low pH suggests that the resulting phenotype switching within biofilm was observed due to the pH gradient of neutral to acidic from the outer to the core of the biofilm. This gradient of H<sup>+</sup> ions concentration may create exploitative stress within the biofilm, which could favor specific pleomorphic cells to thrive in their specialized niches. By understanding the cell size variation in response to the local environment, we propose a model of biofilm formation by pleomorphic cells.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128034"},"PeriodicalIF":6.1,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Streptococcus anginosus orchestrates antibacterial potential of NETs facilitating survival of accompanying pathogens, [Microbiol Res., 2024 Oct 30;290:127959. doi: 10.1016/j.micres.2024.127959]” “血管链球菌协调NETs的抗菌潜力，促进伴随病原体的生存”的更正[j] .微生物研究，2024年10月30日；290:127959。doi: 10.1016 / j.micres.2024.127959]”。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-21 DOI: 10.1016/j.micres.2024.128024

Magdalena Pilarczyk-Zurek , Joanna Budziaszek , Keerthanaa Nandagopal , Aleksandra Kurylek , Aleksandra Kozinska , Michal Dmowski , Izabela Sitkiewicz , Izabela Kern-Zdanowicz , Joanna Koziel

引用次数: 0

Root system architecture plasticity with beneficial rhizosphere microbes: Current findings and future perspectives 有益根际微生物的根系结构可塑性：目前的发现和未来的展望。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-21 DOI: 10.1016/j.micres.2024.128028

Hualiang Zhang , Zilin Liu , Congcong Zheng , Huimin Ma , Ming Zeng , Xuechen Yang

引用次数: 0

Deciphering roles of nine hydrophobins (Hyd1A–F and Hyd2A–C) in the asexual and insect-pathogenic lifecycles of Beauveria bassiana 九种疏水蛋白（Hyd1A-F和Hyd2A-C）在球孢白僵菌无性和虫致病性生命周期中的破译作用。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-20 DOI: 10.1016/j.micres.2024.128029

Jian-Ru Feng, Min Li, Sheng-Hua Ying, Ming-Guang Feng

{"title":"Deciphering roles of nine hydrophobins (Hyd1A–F and Hyd2A–C) in the asexual and insect-pathogenic lifecycles of Beauveria bassiana","authors":"Jian-Ru Feng, Min Li, Sheng-Hua Ying, Ming-Guang Feng","doi":"10.1016/j.micres.2024.128029","DOIUrl":"10.1016/j.micres.2024.128029","url":null,"abstract":"<div><div>Hydrophobins are small amphiphilic proteins that confer filamentous fungal hydrophobicity needed for hyphal growth, development, dispersal and adhesion to host and substrata. In insect-pathogenic <em>Beauveria bassiana</em>, nine hydrophobins (class I Hyd1A–F and class II Hyd2A–C) were proven to localize on the cell walls of aerial hyphae and conidia but accumulate in the vacuoles and vesicles of submerged hyphae and blastospores, respectively. Conidial hydrophobicity, adhesion to insect cuticle, virulence via normal cuticle infection and dispersal potential were significantly more reduced by the <em>hyd1A</em> deletion leading to complete ablation of slender rodlets on conidial coat than the <em>hyd1B</em> deletion, which caused a failure to assemble morphologically irregular rodlets into orderly bundles. Aerial conidiation and submerged blastospore production were compromised in Δ<em>hyd2A</em> and Δ<em>hyd2C</em>. The deletion of <em>hyd1D</em> stimulated conidial germination and virulence via insect hemocoel colonization, which was accelerated in Δ<em>hyd2A</em> but decelerated in Δ<em>hyd2B</em>. However, these deletion mutants were unaffected in radial growth on rich/minimal media and responses to osmotic, oxidative, cell wall-perturbing and heat-shock stresses except for an increase in conidial thermotolerance of Δ<em>hyd1A</em> or cell sensitivity of Δ<em>hyd1B</em> to Congo red-induced stress. None of examined phenotypes was altered in Δ<em>hyd1C</em>, Δ<em>hyd1E</em> and Δ<em>hyd1F</em>. Conclusively, Hyd1A and Hyd1B co-regulate the formation, morphology and orderly assembly of rodlet bundles required for conidial hydrophobicity and infectivity, which are independent of Hyd1C–F and Hyd2A–C in <em>B. bassiana</em>. These results unveil a necessity to distinguish major, minor and dispensable roles among multiple class I/II hydrophobin genes in an ascomycetous pathogen.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128029"},"PeriodicalIF":6.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial, temporal and numerical regulation of polar flagella assembly in Pseudomonas putida 恶臭假单胞菌极性鞭毛组装的时空和数值调控。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-19 DOI: 10.1016/j.micres.2024.128033

Marta Pulido-Sánchez , Antonio Leal-Morales , Aroa López-Sánchez , Felipe Cava , Fernando Govantes

{"title":"Spatial, temporal and numerical regulation of polar flagella assembly in Pseudomonas putida","authors":"Marta Pulido-Sánchez , Antonio Leal-Morales , Aroa López-Sánchez , Felipe Cava , Fernando Govantes","doi":"10.1016/j.micres.2024.128033","DOIUrl":"10.1016/j.micres.2024.128033","url":null,"abstract":"<div><div>The Gram-negative bacterium <em>Pseudomonas putida</em> bears a tuft of flagella at a single cell pole. New flagella must be assembled <em>de novo</em> every cell cycle to secure motility of both daughter cells. Here we show that the coordinated action of FimV, FlhF and FleN sets the location, timing and number of flagella assembled. The polar landmark proteins FimV and FlhF are independently targeted to the nascent new pole during or shortly after cell division, but FimV stabilizes FlhF association with the cell poles. FlhF determines the polar position of the flagella by targeting early flagellar components to the cell pole and preventing their nucleation at non-polar sites. FlhF also promotes efficient flagellar assembly and indirectly stimulates Class III flagellar promoter activation by promoting secretion of the anti-FliA anti-σ factor FlgM. The MinD-like ATPase FleN partitions between the cell poles and the cytoplasm. Cytoplasmic FleN regulates flagellar number by preventing excessive accumulation of FlhF at the cell poles that may otherwise lead to hyperflagellation, likely by antagonizing FleQ-dependent transcriptional activation. FimV is essential to FleN polar location. FimV and FleN temporally regulate the onset of flagellar assembly by preventing premature polar targeting of FlhF and the ensuing premature targeting of additional flagellar components. Our results shed new light on the mechanisms that ensure the timely assembly of the appropriate number of flagella at the correct polar location in polarly flagellated bacteria.</div></div>","PeriodicalId":18564,"journal":{"name":"Microbiological research","volume":"292 ","pages":"Article 128033"},"PeriodicalIF":6.1,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Identification of metabolites produced by six gut commensal Bacteroidales strains using non-targeted LC-MS/MS metabolite profiling” [Microbiol. Res. 283 (2024) 1–11] “使用非靶向LC-MS/MS代谢物分析鉴定六种肠道共生拟杆菌菌株产生的代谢物”的勘误表[微生物杂志]。[Res. 283(2024) 1-11]。

IF 6.1 1区生物学

Microbiological research Pub Date : 2024-12-18 DOI: 10.1016/j.micres.2024.128023

Maria Victoria Fernandez-Cantos , Ambrin Farizah Babu , Kati Hanhineva , Oscar P. Kuipers

引用次数: 0